Fly larvae killer for farms

Abstract

The present invention discloses a fly larvae killer. The fly larvae killer comprises the following substances by weight percentage: 5-10% of S-methoprene, 5-10% of Bacillus, and the remainder of auxiliary materials; wherein the Bacillus includes Bacillus subtilis and Bacillus licheniformis, and the total effective viable count thereof is ≥2×10 10 CFU / g. This fly larvae killer has a clear effect, is easy to use, leaves no residue in the animal body, does not produce harmful metabolites, is green and environmentally friendly, and is of great significance to improving the ecological environment of farms.

Description

Technical Field

[0001] The present invention relates to the field of environmental protection, and in particular to a fly larvae killer for farms. Background Art

[0002] In recent years, people have placed higher and higher demands on the livestock and poultry breeding environment, but there has been no good solution to the rampant flies in farms. The large-scale reproduction and nuisance of flies not only increase the chances of animal disease transmission but also lead to a decline in the production performance of farmed animals, and are becoming an important factor that seriously endangers the sustainable development of the breeding industry. Insecticides used for fly control have been constantly evolving. In the middle of the last century, organic synthetic insecticides, such as organochlorines, organophosphorus, and carbamates, were born one after another; subsequently, the synthesis and application of pyrethroids have made rapid progress. The application of these insecticides has brought many benefits to public health and disease control, but it has also led to the generation and spread of drug resistance in target organisms, as well as toxicity to non-target organisms and environmental pollution. [1] . Since the adult flies in the farms are only the tip of the iceberg of the total number of flies, accounting for only about 20%, it is difficult to completely control the infestation of flies in the farms by simply killing the adults. If you want to fundamentally control the number of flies in the farms, you still have to start with the eradication of the fly larvae. At present, the most commonly used larvicidal drug in farms is cyromazine. It has been nearly 30 years since the Ministry of Agriculture of my country approved the use of cyromazine in the 1990s. Long-term use has gradually caused cyromazine to develop drug resistance, resulting in the need to increase the amount of its addition. Since the metabolite of cyromazine in animals is melamine, this poses a huge hidden danger to food safety. [2] Currently, Japan and European countries have banned the addition of cyromazine to feed. [3] Therefore, my country's animal husbandry industry is in urgent need of finding a fly larvae killer that can replace cyromazine, is safe for humans and animals, has low toxicity, and is environmentally friendly.

[0003] References are as follows:

[0004] [1] Su Tianyun. History, current situation and future of biological mosquito larvae killers[J]. Chinese Journal of Hygiene and Insecticides and Equipment, 2014(1):1-5.

[0005] [2] Wang Hui, Dong Yuanhua, An Qiong. Research progress on biotoxicity and environmental behavior of cyclopropane[J]. Transactions of the Chinese Society of Agricultural Engineering, 2008, 24(1): 246-249.

[0006] [3] Liu Changde, Liu Kai. Diflubenzuron urea granules and preparation method thereof: China, 108902135[P]. 2018.11.30. Summary of the Invention

[0007] To address the shortcomings of existing fly control solutions for farms, the present invention provides a novel fly larvae killer using S-methoprene as its primary ingredient. This fly larvae killer is highly effective, easy to use, leaves no residue in animals, and produces no harmful metabolites. It is environmentally friendly and has significant implications for improving the ecological environment of farms.

[0008] The fly larvae killing agent provided by the invention comprises the following substances in percentage by weight: 5-10% of S-methoprene, 5-10% of Bacillus and the balance of auxiliary materials.

[0009] Wherein, the Bacillus can be Bacillus subtilis and / or Bacillus licheniformis, and the effective viable count of the Bacillus is ≥ 2×10 10 CFU / g.

[0010] The Bacillus is preferably Bacillus subtilis.

[0011] In one embodiment of the present invention, the Bacillus is Bacillus subtilis.

[0012] In another embodiment of the present invention, the Bacillus is a mixture of Bacillus subtilis and Bacillus licheniformis in a mass ratio of 1:1.

[0013] The auxiliary material can be one or a combination of modified starch and / or maltodextrin.

[0014] Specifically, the fly larvae killer of the present invention is any one of the following 1) to 6):

[0015] 1) The fly larvae killer is composed of the following substances in percentage by weight: 5% S-methoprene, 10% Bacillus subtilis, and the balance excipients (such as modified starch);

[0016] 2) The fly larvae killer is composed of the following substances in percentage by weight: 7.5% of S-methoprene, 10% of Bacillus subtilis, and the balance of auxiliary materials (such as modified starch);

[0017] 3) The fly larvae killer is composed of the following substances in percentage by weight: 10% S-methoprene, 10% Bacillus subtilis, and the balance excipients (such as modified starch);

[0018] 4) The fly larvae killer is composed of the following substances in percentage by weight: 10% S-methoprene, 5% Bacillus subtilis, 5% Bacillus licheniformis, and the balance excipients (such as modified starch);

[0019] 5) The fly larvae killer is composed of the following substances in percentage by weight: 10% S-methoprene, 5% Bacillus subtilis, and the balance excipients (such as modified starch);

[0020] 6) The fly larvae killer is composed of the following substances in percentage by weight: 5% of S-methoprene, 5% of Bacillus subtilis, and the balance of auxiliary materials (such as modified starch or maltodextrin).

[0021] Another object of the present invention is to provide a use of the above fly larvae killer.

[0022] The application of the fly larvae killer provided by the present invention is its application in killing mosquitoes and flies in breeding farms.

[0023] The breeding farm is for livestock and poultry breeding, and the livestock and poultry include: chickens (including laying hens and broiler chickens), ducks, geese, pigs, cattle (including dairy cows and beef cattle), sheep, rabbits, etc.

[0024] The present invention also provides a method for using the fly larvae killer.

[0025] The fly larvae killer provided by the present invention is used to kill mosquitoes and flies in livestock and poultry farms. The method can be used by mixing with feed or drinking water, or spraying. The best effect is achieved when the agent is used two weeks before mosquitoes and flies begin to reproduce.

[0026] Specifically, the mixed feed is fed by adding 100-150g of fly larvae killer per ton of complete feed, mixing evenly and then feeding directly;

[0027] When the livestock and poultry are ruminants, the mixed feed feeding can also be carried out in the following manner, that is, 200-500g (specifically 300g) of fly larvae killer is added to each ton of concentrate supplement, mixed evenly and then fed directly.

[0028] When used in drinking water, add 50-80g of fly larvae killer per ton of water and allow livestock and poultry to drink freely.

[0029] When spraying, dissolve the fly larvae killer in water at a mass ratio of 1:5000-10000 (specifically 1:5000), and spray the entire farm continuously for at least three days, and then spray once a week.

[0030] The present invention also provides a method for killing mosquitoes and flies by utilizing the fly larvae killing agent.

[0031] The method for killing mosquitoes and flies provided by the present invention includes the following three methods:

[0032] One method is: the fly larvae killer is mixed evenly with complete feed and then directly fed to livestock and poultry, wherein 100-150g of fly larvae killer is added per ton of complete feed; when the livestock and poultry are broilers, the complete feed is broiler ration.

[0033] Alternatively, the fly larvae killer agent is mixed evenly with a concentrate supplement and then fed to ruminants, wherein 200-500 g (specifically 300 g) of the fly larvae killer agent is added per ton of concentrate supplement.

[0034] Another method is to dissolve the fly larvae killer in water at a mass ratio of 1:5000-10000 (specifically 1:5000), and spray the entire farm continuously for at least three days, and then spray once a week. The entire farm includes the farm working ground and excrement; the spraying amount is 80-100ml / m 2 area.

[0035] Another method is: livestock and poultry freely drink water added with the fly larvae killer; wherein, 50-80g of the fly larvae killer is added per ton of water.

[0036] Compared with the prior art, the present invention has the following beneficial technical effects:

[0037] S-methoprene is an insect juvenile hormone analogue that has no direct toxic effect on adult mosquitoes and flies. After mosquito and fly larvae come into contact with S-methoprene, they can develop to the pupal stage, but cannot normally emerge as adults. Larvae that cannot emerge have no vitality, thereby reducing the number of adult flies in the use environment. Currently, insecticides containing S-methoprene are mostly used in the form of spraying to kill pests in farmland, fields, warehouses, swimming pools, etc., and are rarely used in livestock farms. The present invention provides a product for use in livestock and poultry farms, which can be sprayed or directly fed to livestock and poultry; it is mixed in the diet according to a certain proportion and fed to livestock and poultry, which can not only eliminate fly larvae on the surface of the environment such as diet and feces, but also continue to act in animal feces, deeply killing internal pests, with fast onset, good effect, and simple operation. At the same time, the fly larvae killing agent provided by the present invention contains a certain proportion of Bacillus, which can improve the breeding environment on the one hand, and promote the colonization of probiotics in the animal intestine and improve intestinal health on the other hand. When used in combination with S-methoprene, it can further reduce the breeding of mosquitoes and flies. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 The change in fly numbers after using the product of Example 7 in a pig farm. DETAILED DESCRIPTION

[0039] The present invention is described below by means of specific embodiments, but the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

[0040] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0041] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0042] The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0043] The S-methoprene in the following examples was sourced from Changzhou Shengjie Chemical Co., Ltd. with a purity of 95%.

[0044] Bacillus licheniformis and Bacillus subtilis were obtained from Tianjin Bofeide Technology Co., Ltd., and the effective viable count was not less than 2×10 10 CFU / g.

[0045] Emergence inhibition rate = (number of pupae that failed to emerge / total number of larvae) × 100%

[0046] Example 1

[0047] By weight percentage, 5.0% of S-methoprene, 10% of Bacillus subtilis and the balance (85%) of modified starch are uniformly mixed to prepare a fly larvae killer.

[0048] Example 2

[0049] By weight percentage, 7.5% of S-methoprene, 10% of Bacillus subtilis and the balance (82.5%) of modified starch are uniformly mixed to prepare a fly larvae killer.

[0050] Example 3

[0051] By weight percentage, 10% of S-methoprene, 10% of Bacillus subtilis and the balance (80%) of modified starch are evenly mixed to prepare a fly larvae killer.

[0052] Example 4

[0053] By weight percentage, 10% of S-methoprene, 10% of Bacillus (5% by weight of Bacillus licheniformis and 5% by weight of Bacillus subtilis) and the balance (80%) of modified starch are uniformly mixed to prepare a fly larvae killer.

[0054] Example 5

[0055] By weight percentage, 10% of S-methoprene, 5% of Bacillus subtilis and the balance (85%) of modified starch are uniformly mixed to prepare a fly larvae killing agent.

[0056] Example 6

[0057] By weight percentage, 5% of S-methoprene, 5% of Bacillus subtilis and the balance (90%) of maltodextrin were uniformly mixed to prepare a fly larvae killer.

[0058] Comparative Example 1

[0059] By weight percentage, 0% of S-methoprene, 10% of Bacillus subtilis and the balance (90%) of modified starch are uniformly mixed to prepare a fly larvae killer.

[0060] Comparative Example 2

[0061] By weight percentage, 2.5% of S-methoprene, 10% of Bacillus subtilis and the balance (87.5%) of modified starch were uniformly mixed to prepare a fly larvae killing agent.

[0062] Application effect test:

[0063] The fly larvae killing products prepared in Examples 1-6 and Comparative Examples 1-2 were evenly mixed into the broiler diet at a rate of 100 grams per ton of complete feed. The broilers were fed these diets. After 10 days, the feces collection pans were cleaned and 100 grams of fresh feces were collected from each group. The collected feces were placed in a fly control room, and the pupation and emergence of fly larvae were observed. The results are shown in Table 1.

[0064] Table 1 Effects of different fly larvae killers on the emergence rate of fly larvae

[0065] Group Emergence inhibition rate Example 1 86.8％ Example 2 94.2％ Example 3 100％ Example 4 97.8％ Example 5 95.5％ Example 6 85.3％ Comparative Example 1 0％ Comparative Example 2 35.6％

[0066] The above results show that at concentrations of 5.0% or higher, S-methoprene can inhibit fly larvae emergence by over 85%. This result significantly reduces fly populations on farms. In actual use, the S-methoprene dosage can be adjusted based on the fly population on the farm.

[0067] According to the regulations made by the Joint Food and Agriculture Organization / World Health Organization (WHO / FAO) Meeting on Pesticide Residues (JMPR) in 2001, the acceptable daily intake of S-methoprene is 0.05 mg / kg body weight.

[0068] Example 7

[0069] Mix 5% S-methoprene, 5% Bacillus subtilis, and 90% maltodextrin by weight. Set up two pig farms with similar environments and scales, one as a blank control and the other as a treatment group. The treatment method for the treatment group is to dissolve the mixed product in water at a ratio of 1:5000 (mass ratio). Then spray it directly on the working ground and excrement of the farm until the surface is wet, with a water volume of about 80ml / m 2 -100ml / m 2The area can be used. When using for the first time, use it for 3 consecutive days, and then spray it every 7 days. Place fly traps at fixed locations inside and outside the farm. At sunrise, place the baited fly traps at the sampling point to attract flies. At sunset, collect the traps, kill the live flies with insecticide, and count them. The collected data is as follows: Figure 1 shown.

[0070] Depend on Figure 1 It can be seen that the number of flies in the treatment group decreased significantly after about 15 days, while the number of flies in the control group remained basically unchanged.

Claims

1. A fly larvae killer for livestock and poultry farms, comprising the following substances in percentage by weight: 10% S-methoprene, 10% Bacillus, and the remainder excipients; The Bacillus is Bacillus subtilis, and its total effective viable count is ≥ 2×10 10 CFU / g; The auxiliary material is one or a combination of modified starch and / or maltodextrin.

2. Use of the fly larvae killer according to claim 1 for killing mosquitoes and flies in a breeding farm; the breeding farm is a livestock and poultry farm.

3. The use according to claim 2, characterized in that: The livestock and poultry include chickens, ducks, geese, pigs, cattle, sheep and rabbits.

4. The method for using the fly larvae killer according to claim 1, comprising the following 1) or 2) or 3) : 1) Mix the fly larvae killer with the feed evenly and feed it directly to livestock and poultry; 2) Mix the fly larvae killer with drinking water and allow livestock and poultry to drink freely; 3) Prepare the fly larvae killer into a solution and spray it; In the above 1), the feed is a complete feed, and 100-150g of fly larvae killer is added to each ton of the complete feed, mixed evenly and then fed directly; Alternatively, the livestock and poultry are ruminants, and the feed is a concentrate supplement, 200-500 g of fly larvae killer is added to each ton of concentrate supplement, mixed evenly, and then fed directly; In the above 2), 50-80 g of fly larvae killer is added per ton of drinking water; In the above 3), the fly larvae killing agent is dissolved in water at a mass ratio of 1:5000-10000, and the entire farm is sprayed continuously for at least three days, and then sprayed once a week; the entire farm includes the farm operation ground and excrement; the spraying amount is 80-100ml / m 2 area; The fly larvae killer is used 2 weeks before mosquitoes and flies begin to reproduce.

5. A method for killing mosquitoes and flies using the fly larvae killer according to claim 1, comprising the following steps A) or B) or C): A) Mix the fly larvae killer with feed and feed it directly to livestock and poultry to kill mosquitoes and flies; B) Mix the fly larvae killer with drinking water and allow livestock and poultry to drink freely, thereby killing mosquitoes and flies; C) preparing the fly larvae killer into a solution and spraying it to kill mosquitoes and flies; In A), the feed is a complete feed, and 100-150g of fly larvae killer is added to each ton of the complete feed, mixed evenly and then fed directly; Alternatively, the livestock and poultry are ruminants, and the feed is a concentrate supplement, 200-500 g of fly larvae killer is added to each ton of concentrate supplement, mixed evenly, and then fed directly; In B), 50-80 g of fly larvae killer is added per ton of drinking water; In said C), the fly larvae killing agent is dissolved in water at a mass ratio of 1:5000-10000, and the entire farm is sprayed continuously for at least three days, and then sprayed once a week; the entire farm includes the farm operation ground and excrement; the spraying amount is 80-100ml / m 2 area; The fly larvae killer is used 2 weeks before mosquitoes and flies begin to reproduce.

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