An integrated bacteria and algae cultivation and purification device for factory circulating water

Abstract

A bacteria-algae integrated culture and purification device for factory circulating water comprises a culture box, an aeration pipe installed in the culture box, and a second culture rack and a first culture rack installed in the culture box, which are respectively located on the upper and lower sides of the aeration pipe, and the second culture rack and the first culture rack are each provided with at least two flat plates for installing a plurality of bags with the same spacing and bacteria-algae balls placed inside. The device of the present invention rationally plans the growth space of the bacteria-algae balls, ensuring the activity of the bacteria-algae balls; at the same time, the device can transfer the bacteria-algae balls as a whole, with a short transfer time, thereby improving the survival rate of the bacteria-algae balls, so the device has a good water quality treatment effect and a long duration.

Description

Technical Field

[0001] The present invention belongs to the technical field of factory circulating water treatment, and in particular relates to a bacteria-algae integrated cultivation and purification device for factory circulating water. Background Art

[0002] Factory-scale recirculating aquaculture has the advantages of not being restricted by the external environment, saving land and water, being environmentally friendly, producing safe and reliable aquatic products, and achieving high-density farming. It has become the future development direction of the aquaculture industry.

[0003] Intensive high-density aquaculture, represented by circulating water factory farming, integrates modern engineering, electromechanical, biological, environmental protection, feed science and other disciplines. By creating good water and environmental conditions suitable for fish growth and reproduction, and equipped with scientific intensive farming technology, it achieves the purpose of shortening the breeding cycle, reducing feed consumption, saving labor, reducing costs and increasing unit area production. At present, countries such as Japan, Australia, Germany, the United Kingdom, and the United States all use different forms of closed water circulation systems for factory aquaculture. China has begun to demonstrate and promote factory fish farming and large-scale circulating water aquaculture since the early 1990s, but it still lags behind developed countries in related facilities, equipment, intensive farming technology, etc. The lack of key technologies and supporting development capabilities for high-level closed circulating water aquaculture systems is an important factor.

[0004] Water quality is the root and foundation of aquaculture, directly affecting the quality, efficiency and even the success or failure of aquaculture. The core technology of high-density circulating aquaculture is water treatment technology, and biological purification, namely biofilter (bioreactor) technology, is the key to the successful operation of all (seawater, freshwater) closed-circulation water treatment systems. Biofilters play an important role in controlling toxic and harmful substances such as COD, NH4+, NO2-, NO3- in aquaculture water, and their effectiveness has been proven by many research results. Many factors can affect the efficiency of biofilters, such as operating conditions, processes and other factors, but the decisive influencing factors are the number of microorganisms present in them and their efficiency. In factory-scale high-density aquaculture with circulating water, a higher circulation frequency of aquaculture water is generally required. The circulating water workshops in some developed countries generally circulate more than 12 times a day, while the circulation frequency in circulating water workshops in other countries ranges from 4 to 8 times. The volume of the biological filter used for water purification is generally only 1 / 10 to 1 / 30 of the volume of the aquaculture water. When the hydraulic retention time is usually only 30 to 60 minutes, in order to achieve a removal efficiency of more than 50% for COD, NH4+, NO3-, etc., it is necessary to develop high-efficiency water purification bacteria that match the circulating water treatment system and biological filter.

[0005] The existing technology for water purification bacterial agents focuses on developing the purification effect of bacterial agents. For example, the document with the authorization number CN101475265B discloses a water purification method and a composite bacterial agent for a circulating water factory aquaculture system. The composite bacterial agent is first expanded and cultured, and then the composite bacterial agent is directly applied to the factory circulating water workshop as a biofilm. The method has a slow culture speed, and when the water is purified in the circulating water workshop, the growth of the bacterial agent in the water body will cause factors that are not conducive to the growth of the bacterial agent, such as high carbon dioxide concentration and low water pH value. The fluctuation range of carbon dioxide concentration and water pH value is large, which is not conducive to artificial adjustment.

[0006] Therefore, the inventors use bacteria and algae that are easier to fix and cultivate as the main body of water purification, and cooperate with the measures of separating the factory breeding workshop from the water quality treatment workshop to solve the above problems: first prepare laboratory-scale bacteria and algae pellets for testing, and after the test is passed, prepare a large number of bacteria and algae pellets and place them in the water quality treatment workshop; when the water quality in the factory breeding workshop exceeds the standard, the water in the breeding workshop is discharged to the water quality treatment workshop and the breeding workshop is replenished with water. After the bacteria and algae pellets treat the discharged water to meet the water quality standards, the treated water is re-injected into the breeding workshop to form a cycle. However, in this design, the bacteria and algae pellets need to be transferred after preparation and then fixed in the water quality treatment workshop. During the transfer and fixation process, the problem of reduced purification efficiency of the bacteria and algae pellets due to environmental changes needs to be solved urgently. Summary of the invention

[0007] In view of the above-mentioned problems, the purpose of the present invention is to provide an integrated bacteria and algae cultivation and purification device for factory circulating water, which rationally plans the growth space of the bacteria and algae balls to ensure the activity of the bacteria and algae balls; at the same time, the device can transfer the bacteria and algae balls as a whole with a short transfer time, thereby improving the survival rate of the bacteria and algae balls. Therefore, the water quality treatment effect of the device is good and the duration is long.

[0008] In order to achieve the above object, the technical solution of the present invention is as follows:

[0009] A bacteria-algae integrated culture and purification device for factory circulating water comprises a culture box, an aeration pipe installed in the culture box, and a second culture rack and a first culture rack installed in the culture box, which are respectively located on the upper and lower sides of the aeration pipe, wherein the second culture rack and the first culture rack are each provided with at least two flat plates for installing a plurality of placement bags with the same spacing and placed inside with bacteria and algae balls.

[0010] In the design of this scheme, firstly, a plurality of identical placement bags are reasonably designed to be installed on a flat plate, and a plurality of flat plates are installed on a culture rack at the same intervals, and two culture racks are distributed on both sides of the aeration pipe, thereby ensuring that the growth environment of each bacteria and algae ball is almost the same and the density is uniform, avoiding the problem of uneven growth state of bacteria and algae balls at different depths and uneven density of bacteria and algae balls causing death of bacteria and algae balls in the traditional culture mode, improving the survival rate of bacteria and algae balls and ensuring the activity of bacteria and algae balls in combination with conventional nutrient inputs; at the same time, the device is composed of a plurality of independent culture boxes connected by aeration pipes to form a whole, which can be suitable for water quality treatment in different spaces, is simple to install, has strong adaptability, and has good application prospects.

[0011] As a further preferred embodiment of the present invention, both ends of each of the flat plates are installed in installation grooves corresponding to the side ends of the first culture rack and the second culture rack, and a screen plate with a screen is installed on the upper surface of each of the flat plates for reinforcing the placement bag and limiting the bacterial and algae balls.

[0012] In the design of this scheme, a detachable sieve plate is used to seal the bag after the bacteria and algae balls are placed. This ensures that during cultivation, the bacteria and algae balls are confined to a fixed space for growth and will not migrate with the flow of water, thereby controlling the density and spatial distribution of the entire device. This design is also conducive to grouping the bacteria and algae balls for easy observation and recording, further improving the control effect of water quality treatment.

[0013] As a further preference of the present invention, a fill light component installed on the inner wall of the first culture rack or the second culture rack is provided between two adjacent flat plates. The design of the fill light component ensures that in the case of insufficient light or the bacteria and algae balls at deep depths and the bacteria and algae balls at shallow depths receive sufficient light, thereby improving the survival rate and activity of the bacteria and algae balls.

[0014] As a further preferred embodiment of the present invention, the lower surface of the sieve plate body is provided with mounting columns adapted to column grooves correspondingly formed on the upper surface of the flat plate.

[0015] As a further preferred embodiment of the present invention, protrusions for increasing friction are provided on the lower surface of the sieve plate body at both side ends of the mounting posts.

[0016] As a further preferred embodiment of the present invention, the frames of the first culture rack and the second culture rack are respectively installed in the first fixing groove and the second fixing groove correspondingly opened in the culture box, and the culture box is also provided with a fixing column which passes through the frame of the second culture rack to strengthen and fix the second culture rack.

[0017] As a further preferred embodiment of the present invention, air-permeable mesh plates for limiting the aeration tube are installed at the upper and lower sides of the aeration tube in the culture box. The design of the air-permeable mesh plates not only limits the installation of the aeration tube, but also does not affect the gas delivery to the culture rack.

[0018] As a further preferred embodiment of the present invention, the culture box includes a mounting frame and a filter screen arranged on the mounting frame, and openings are formed on the filter screen located on two sides where the aeration pipe is installed.

[0019] As a further preferred embodiment of the present invention, both ends of the fill light component are connected to fixing boxes fixed on the inner side wall of the first culture rack or the second culture rack.

[0020] As a further preferred embodiment of the present invention, a power supply for supplying power to the fill light component is arranged in the fixing box.

[0021] In summary, the present invention has the following beneficial effects:

[0022] The device of the present invention ensures that the growth environment of each bacteria and algae ball is almost consistent and the density is uniform, and the bacteria and algae balls can be quickly transferred as a whole, thereby improving the survival rate of the bacteria and algae balls.

[0023] The device of the present invention is composed of a plurality of independent culture boxes connected by aeration pipes to form a whole, can be applied to water treatment in different spaces, has simple installation, strong adaptability, and has good application prospects.

[0024] The movement and installation of the device structure of the present invention does not require any electric control equipment or air pumps or other adjusting parts, thereby reducing production costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Attached Figure 1 It is a structural schematic diagram of the present invention.

[0026] Attached Figure 2 It is a schematic diagram of the structure of the culture rack of the present invention.

[0027] Attached Figure 3 It is a partial structural schematic diagram of the culture rack of the present invention.

[0028] Attached Figure 4 It is a schematic diagram of a partial three-dimensional structure of the culture rack of the present invention.

[0029] Attached Figure 5 It is a schematic diagram of the structure of the incubator of the present invention.

[0030] Description of the drawings: incubator 1, mounting frame 11, filter screen 12, opening 121, first fixed slot 101, second fixed slot 102, fixed column 103, aeration tube 2, culture unit 3, first culture rack 31, mounting slot 311, fixed box 312, second culture rack 32, flat plate 33, column slot 331, placement bag 4, fill light 5, breathable mesh plate 6, fixing frame 61, breathable mesh 62, bacteria and algae ball 7, sieve plate 81, sieve screen 82, mounting column 83, protrusion 84. DETAILED DESCRIPTION

[0031] As attached Figure 1 , 5 As shown, the bacteria-algae integrated culture and purification device used in this embodiment includes a plurality of culture boxes 1 placed in a water treatment workshop and culture units 3 placed in the culture boxes 1. Each culture box 1 is composed of a total of four mounting frames 11 on the left and right and front and back and a filter screen 12 fixed on the mounting frame 11 to form a water-permeable and air-permeable mesh box structure. The middle space in the box body of each culture box 1 is separated by two identical air-permeable mesh panels 6 to form a space for installing an aeration pipe 2, and openings 121 are correspondingly opened on the filter screens 12 on the left and right sides of the culture box 1 to allow the aeration pipe 2 to pass through. The size of the opening 121 only needs to be slightly larger than the aeration pipe, and a fixing ring (not shown in the figure) can be added at the opening 121 to reinforce the installation of the aeration pipe; in this embodiment, the air-permeable mesh panel 6 is composed of a fixing frame 61 fixedly installed inside the box body of the culture box 1 and an air-permeable mesh 62 fixedly installed on the fixing frame 61. In this embodiment, the air permeable mesh plate 6 separates the interior of the incubator 1 into an upper space and a lower space, so the second culture rack 32 and the first culture rack 31 of the same structure are respectively installed in the upper space and the lower space. Figures 1 to 4As shown, taking the first culture rack 31 as an example, it is mainly composed of a rack body, a flat plate 33 installed on the left and right side surfaces of the rack body, a plurality of placement bags 4 installed on the flat plate 33 with the same intervals, a sieve plate 81 with a sieve 82 detachably installed on the flat plate 33 for cooperating with the placement bags 4 to form a space for placing the bacteria and algae balls 7, and a fill light component 5 installed on the left and right side surfaces of the rack body and located between two adjacent flat plates 33. The two ends of the flat plate 33 are respectively inserted into the installation grooves 311 opened on the left and right sides of the first culture rack 31, and the main body of the flat plate 33 is provided with a plurality of openings of the same size as the opening of the placement bag 4, and the openings of the placement bag 4 can be directly fixed on the openings opened on the flat plate 33; the lower surface of the sieve plate 81 is provided with installation columns 83 inserted into the four corresponding column grooves 331 opened on the flat plate 33, and in order to prevent the sieve plate 81 from falling off on the second culture rack 32 due to gravity, a plurality of protrusions 84 are installed on the lower surface of the flat plate 33 at both ends of the installation columns 83, and the protrusions 84 are made of rubber material with greater friction, and the cross section is an inferior arc shape to reduce the gap; the fill light 5 is an existing waterproof lamp with a light intensity of 3000lux-4800lux, and the waterproof structure includes a lamp body and a photosensitive chip, the photosensitive chip controls the current of the lamp body, and the lamp body uses a light strip composed of LED lamp beads, and the light compensation component sensed by the light source can automatically adjust the light intensity according to the natural light intensity outside, so as to make full use of natural light and save energy. As shown in the attached Figure 5 As shown, the first culture rack 31 is fixed to the lower mounting rack 11 by means of a mounting cross bar (not shown in the figure) provided at the lower end which can be embedded in a first fixing groove 101 correspondingly provided on the mounting rack 11 at the lower end; the mounting rack 11 at the upper end and the second culture rack 32 have the same structure as the mounting rack 11 at the lower end and the first culture rack 31, and additionally a fixing column 103 for reinforcing the second culture rack 32 installed in a hoisting manner is installed on the mounting rack 11 at the upper end. Therefore, the second culture rack 32 is not only provided with the same mounting cross bar as the first culture rack 31, but also has a corresponding channel (not shown in the figure) for embedding the fixing column 103.

[0032] This embodiment also provides a method for preparing a bacterial algae ball: 1. Under aseptic operation conditions, inoculate the four-tailed scenedesmus or protein nucleus chlorella in SE culture medium at an inoculation amount of 1×106 / mL×107 / mL, and culture at a temperature of 25°C-27°C, a pH value of 6.5-7, a rotation speed of 110-130r / min, and alternately culture with light and dark cycles, with a light intensity of 3000-4000Lx. After culturing for 60-75h, obtain a microalgae culture solution, centrifuge at 5000-6000r / min for 5-10min, wash with distilled water, centrifuge to remove inorganic salts and other substances attached to the algae cells, and store at 4°C for standby use. 2. Take a certain volume of activated sludge from a municipal sewage treatment plant, dilute the activated sludge with sterile water, apply it to a bromothymol blue (BTB) plate culture medium, and culture it at 30°C for 2d. Select the blue colonies on the plate, separate and purify them by streaking on beef extract peptone plate medium, inoculate them into heterotrophic nitrification culture medium, shake and culture them at 30℃ for 2 days, and the strains with significantly reduced ammonia nitrogen concentration in the culture medium are heterotrophic nitrification-aerobic denitrification bacteria. Inoculate 10% (volume ratio) of the bacterial solution in the logarithmic growth phase into a conical flask of DM denitrification medium, culture it at 30℃ and 160 r / min overnight, centrifuge and concentrate it at 8000 r / min, and store it at 4℃ for later use. 3. Mix the centrifuged concentrated algae solution and bacterial solution in a ratio of 1:1 to prepare a composite bacterial algae suspension. 4. Weigh a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) and mix them with water. The concentration of polyvinyl alcohol (PVA) is 2% and the concentration of sodium alginate is 3%. Heat it to 100℃ in a water bath until it is completely dissolved and then cool it to room temperature. 5. Add the prepared composite bacteria-algae suspension to the cooled polyvinyl alcohol (PVA) and sodium alginate (SA) mixture at a ratio of 30:6 (volume ratio) and mix well. 6. Suck the above mixture into a syringe and slowly drip into a 3% calcium chloride solution. Soak the formed pellets in the calcium chloride solution and place them at 20°C for 4 hours before taking them out. Wash them with distilled water 3 times and place them in a culture dish to dry to obtain the immobilized pellets.

[0033] The principle of this embodiment is: the cultured algae balls 7 are evenly distributed in the placement bags 4 pre-installed in the first culture rack 31 / second culture rack 32, and the sieve plates 81 of each layer are fixed on the flat plate 33. At this time, the culture racks are installed. The first culture rack 31 and the second culture rack 32 are respectively installed in the culture box 1 with five sides, namely the left, right, top and bottom, and the back side, which are pre-assembled and installed with the filter screen 12. After the two breathable mesh plates 6 are installed and the aeration pipe 2 is installed and passed through the left and right sides of the culture box, the mounting frame 11 with the filter screen 12 installed is also installed on the front face of the culture box 1. The entire culture box 1 is installed. Repeat the above steps to complete the installation of multiple culture boxes 1, and connect the aeration pipes 2 at the side ends of each culture box 1 to complete the installation of the purification device of the entire water treatment workshop. After the equipment is completed, the excess water in the breeding workshop can be transported to the water treatment workshop for purification.

[0034] The growth space of the bacteria and algae balls in the purification device of the present invention is reasonably planned and can be quickly and integrally transferred, which greatly improves the survival rate and growth rate of the bacteria and algae balls, so the water purification effect is good and the treatment time is long.

[0035] The inventors tested the water purification effect: first, soak the commonly used fish farming extruded feed on the market in water for 2 hours, remove the residue, dilute, and prepare two simulated aquaculture wastewaters, the first group: COD of 550 mg / L, ammonia nitrogen concentration of 110 mg / L and the second group: COD of 55 mg / L, ammonia nitrogen concentration of 11 mg / L, and lay the purification device incubator with 5% bacterial algae ball inoculation. The purification effect after 24h, 36h, 48h, 96h, 7d, 14d, and 30d of culture was measured, and the data are as follows:

[0036]

[0037] As shown in the above table, the COD and ammonia nitrogen concentrations in high-concentration and low-concentration simulated aquaculture wastewater can be completely removed, and the water purification effect of the device can be stabilized to about 30 days (the actual removal rate can still reach about 67% at 40 days), and no nitrite nitrogen and nitrate nitrogen were detected in the water quality test, which further proves that the bacteria and algae integrated culture and purification device adopted in the present invention has good water purification effect and long treatment time.

[0038] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A bacteria-algae integrated culture and purification device for factory circulating water, characterized in that: The invention comprises a culture box (1), an aeration pipe (2) installed in the culture box (1), and a second culture rack (32) and a first culture rack (31) installed in the culture box (1) and located at the upper and lower sides of the aeration pipe (2), respectively; the second culture rack (32) and the first culture rack (31) are each provided with at least two plates (33) for installing a plurality of placement bags (4) with the same spacing and containing bacterial and algal balls (7) therein; A permeable mesh plate (6) for limiting the position of the aeration pipe (2) is installed in the culture box (1) at the upper and lower sides of the aeration pipe (2); The culture box (1) comprises a mounting frame (11) and a filter screen (12) arranged on the mounting frame (11), and openings (121) are provided on the filter screen (12) located on two side surfaces where the aeration pipe (2) is mounted; Both ends of each of the flat plates (33) are mounted in mounting grooves (311) correspondingly provided at the side ends of the first culture rack (31) and the second culture rack (32), and a sieve plate (81) with a sieve (82) is mounted on the upper surface of each of the flat plates (33) for reinforcing the placement bag (4) and limiting the position of the bacterial and algal balls (7).

2. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 1 is characterized in that: A fill light component (5) mounted on the inner side wall of the first culture rack (31) or the second culture rack (32) is provided between two adjacent flat plates (33).

3. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 1 is characterized in that: The lower surface of the sieve plate (81) body is provided with a mounting column (83) adapted to a column groove (331) correspondingly formed on the upper surface of the flat plate (33).

4. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 3 is characterized in that: The lower surface of the main body of the sieve plate (81) is provided with protrusions (84) for increasing friction at positions on both side ends of the mounting column (83).

5. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 1 is characterized in that: The frame bodies of the first culture rack (31) and the second culture rack (32) are respectively installed in the first fixing groove (101) and the second fixing groove (102) correspondingly opened in the culture box (1), and the culture box (1) is also provided with a fixing column (103) which passes through the frame body of the second culture rack (32) to reinforce and fix the second culture rack (32).

6. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 2 is characterized in that: Both ends of the fill light component (5) are connected to fixing boxes (312) fixed on the inner side wall of the first culture rack (31) or the second culture rack (32).

7. The integrated bacteria and algae culture and purification device for factory circulating water according to claim 6 is characterized in that: A power source for supplying power to the fill light component (5) is arranged in the fixing box (312).

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