Cell analysis equipment, methods for analyzing cells, and computer-readable storage media

Abstract

This invention provides a cell analysis device and method that acquires digital images of cells in a sample and images that characterize the appearance of the sample; and outputs and displays the digital images of cells in the sample and images that characterize the appearance of the sample. This allows users to conveniently monitor the working status of the cell analysis device and observe whether the appearance of blood smears is normal.

Description

Technical Field

[0001] This invention relates to medical device technology, and more particularly to a method for analyzing cells, a cell analysis device, and a computer-readable storage medium. Background Technology

[0002] A blood cell analyzer is an instrument that detects cells in the blood, counting and classifying cells such as white blood cells (WBC), red blood cells (RBC), platelets (PLT), nucleated red blood cells, and reticulocytes. In recent years, blood cell analysis has evolved towards automation and intelligent detection. Advanced testing instruments and experimental methods have greatly improved the efficiency and economic benefits of hospital laboratories, making test results more accurate and precise. However, microscopic examination of blood smear cell morphology remains the gold standard for assessing pathological changes in blood cell morphology. Microscopic examination includes observing inflammatory changes in white blood cell toxicity, pathological changes in leukemia cells, morphological changes in anemic red blood cells, platelet morphological characteristics, and examination for blood parasites. These cannot be completely replaced by any blood cell analyzer.

[0003] Traditional manual microscopic examination methods are time-consuming and labor-intensive, and require highly skilled operators. However, there has been a severe shortage of qualified morphological microscopists in recent years. These factors have resulted in a significantly low rate of manual microscopic examination in many medical institutions, consequently affecting the quality of laboratory reports. Currently, fully automated digital image analysis systems for blood cells have been introduced. These systems automate blood smear uploading, single-cell layer and cell localization, and cell image scanning, significantly improving detection speed and cell image quality. The system displays all counted white blood cells by category on the computer screen, and reports can be issued after review or reclassification by the examiner.

[0004] Compared to manual microscopic examination, digital cell analysis systems automatically select the working area, capture and photograph cells, greatly improving the efficiency of microscopic examination. However, precisely because all of these tasks are completed automatically by the instrument, laboratory technicians cannot monitor the operating status of the analysis system. Summary of the Invention

[0005] This application provides a cell analysis device, a method for analyzing cells, and a computer-readable storage medium. The technical solution proposed in this application allows users to monitor the working status of the analysis device, monitor whether the captured cell images come from a suitable sample (such as a blood smear), and monitor the appearance and morphology of the sample (such as a blood smear).

[0006] The technical solution of this invention is implemented in the following manner.

[0007] This invention provides a cell analysis device, the cell analysis device comprising:

[0008] A control device configured to adjust the relative position of the digital imaging device and the sample;

[0009] Digital imaging apparatus, including lens group and digital camera;

[0010] An image acquisition device is configured to acquire digital images of cells in a sample and images that characterize the appearance and morphology of the sample.

[0011] The display output device is configured to output digital images of the cells and images that characterize the appearance and morphology of the sample.

[0012] In another embodiment, the image that characterizes the appearance of the sample is a digital image captured by the digital camera, or a simulated image that characterizes the appearance of the sample.

[0013] In another embodiment, the control device is further configured to acquire the shooting location information of the digital image of the cell in the sample, and obtain information about the corresponding position in the image that characterizes the appearance morphology of the sample based on the shooting location information.

[0014] In another embodiment, the display output device is further configured to: output a digital image of the cell and an image characterizing the appearance of the sample, and output the corresponding position in the image characterizing the appearance of the sample.

[0015] In another embodiment, the display output device is also configured to output objective lens information.

[0016] In another embodiment, the method of outputting the corresponding position in the image that characterizes the appearance of the sample includes: identifying the corresponding position using at least one of graphics, text, and numbers; optionally, the size of the identifier is consistent with the size of the digital image of the corresponding cell.

[0017] In another embodiment, the output objective information includes output display objective information using at least one of color, graphics, text, and numbers.

[0018] In another embodiment, the display output device is further configured to output objective lens information, wherein when the corresponding position is identified using at least one of the graphics, text, and numbers, the objective lens information is represented by the color, line thickness, or solid / darkness of at least one of the graphics, text, and numbers.

[0019] In another embodiment, outputting the corresponding position in the image that can characterize the appearance of the sample includes: updating and outputting the corresponding position in the image that can characterize the appearance of the sample; and retaining the position corresponding to the previously captured path.

[0020] In another embodiment, if the positions corresponding to the previous shooting path are retained, an identifier indicating the order in which each corresponding position is displayed is output.

[0021] In another embodiment, the identifier is at least one of arrows, numbers, and letters.

[0022] In another embodiment, the control device is further configured to receive location information in an image that characterizes the appearance of a sample, and control the display output device to output a digital image of the corresponding cells in the sample.

[0023] In another embodiment, the display output device is configured to output a digital image of the cell and an image characterizing the appearance morphology of the sample on the same display interface.

[0024] This invention also provides a method for analyzing cells, applied to a cell analysis device, the method comprising:

[0025] Acquire digital images of cells in the sample and images that characterize the appearance and morphology of the sample;

[0026] The digital image of the cell is obtained from the location information of the cell in the sample, and the information of the corresponding position in the image that can characterize the appearance of the sample is obtained based on the location information.

[0027] The output displays digital images of the cells in the sample and images that characterize the appearance of the sample, and displays the corresponding positions in the images that characterize the appearance of the sample.

[0028] In another embodiment, the image that characterizes the appearance of the sample is a digital image captured by a digital camera, or a simulated image that characterizes the appearance of the sample.

[0029] Another embodiment also includes outputting and displaying objective lens information.

[0030] In another embodiment, the method of outputting the corresponding position in the image that characterizes the appearance of the sample includes: identifying the corresponding position using at least one of graphics, text, and numbers; optionally, the size of the identifier is consistent with the size of the digital image of the cell.

[0031] In another embodiment, the output display of objective lens information includes output display of objective lens information using at least one of color, graphics, text, and numbers.

[0032] Another embodiment further includes outputting objective lens information, wherein when the corresponding position is identified using at least one of the graphics, text, and numbers, the objective lens information is represented by the color, line thickness, or solid / darkness of the at least one of the graphics, text, and numbers.

[0033] In another embodiment, the output displays a digital image of the cells in the sample and an image that characterizes the appearance of the sample, including: updating the corresponding position in the image that characterizes the appearance of the sample; optionally, retaining the corresponding position of the previously captured path.

[0034] In another embodiment, if the positions corresponding to the previously captured path are retained, an identifier indicating the display order of each corresponding position is output.

[0035] In another embodiment, the identifier is at least one of arrows, numbers, and letters.

[0036] Another embodiment further includes receiving any location information in an image that can characterize the appearance of a sample, and outputting a digital image of the corresponding cells in the sample based on the location information.

[0037] Another embodiment also includes outputting digital images of the cells in the sample and images that characterize the appearance and morphology of the sample on the same display interface.

[0038] This invention also provides a method for analyzing cells, applied to a cell analysis device, the method comprising:

[0039] Acquire digital images of cells in the sample and images that characterize the appearance and morphology of the sample;

[0040] The output displays digital images of the cells in the sample and images that characterize the appearance of the sample; the digital images of the cells in the sample and images that characterize the appearance of the sample are also output on the same display interface.

[0041] Another embodiment further includes: acquiring the shooting location information of the digital image of the cell in the sample, and obtaining information about the corresponding position in the image that can characterize the appearance morphology of the sample based on the shooting location information.

[0042] In another embodiment, the output displaying digital images of cells in the sample and images that characterize the appearance of the sample further includes: outputting digital images of cells in the sample and images that characterize the appearance of the sample, and outputting the corresponding positions in the images that characterize the appearance of the sample.

[0043] This invention also provides a cell analysis device, the cell analysis device comprising:

[0044] Memory configured to store executable instructions;

[0045] When the processor is configured to run executable instructions stored in the memory, it performs the cell analysis method described in any of the above embodiments.

[0046] This invention also provides a computer-readable storage medium storing executable instructions configured to cause a processor to execute the executable instructions to implement the cell analysis method described in any of the above embodiments.

[0047] In this embodiment of the invention, by acquiring digital images of cells in a sample and images that characterize the sample's appearance, and by outputting and displaying these digital images and images that characterize the sample's appearance, users can conveniently monitor the working status of the cell analysis equipment and observe whether the appearance of samples, such as blood smears, is normal. Furthermore, the corresponding positions can be output in the images that characterize the sample's appearance, thus facilitating users to observe whether the cell images were taken from appropriate sample (e.g., blood smear) locations. Attached Figure Description

[0048] Figure 1 This is a schematic diagram of the structure of a cell analysis device in one embodiment;

[0049] Figure 2 This is a schematic diagram of an image display in one embodiment;

[0050] Figure 3 This is a schematic diagram of the structure of a cell analysis device in one embodiment;

[0051] Figure 4 This is a schematic diagram of an image display in another embodiment;

[0052] Figure 5 This is a schematic diagram of an image display in another embodiment;

[0053] Figure 6 This is a schematic diagram of an image display in another embodiment;

[0054] Figure 7 This is a schematic diagram of an image display in another embodiment;

[0055] Figure 8 This is a schematic diagram of an image display in another embodiment;

[0056] Figure 9 This is a schematic diagram of the structure of a cell analysis device in one embodiment;

[0057] Figure 10 This is a schematic diagram of the cell analysis system in one embodiment;

[0058] Figure 11 This is a flowchart illustrating a method for analyzing cells in one embodiment;

[0059] Figure 12 This is a flowchart illustrating a method for analyzing cells in one embodiment;

[0060] Figure 13 This is a schematic diagram of the structure of a cell analysis device in one embodiment. Detailed Implementation

[0061] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings. This invention should not be construed as limited to the provided embodiments. On the contrary, the content described in the embodiments of this invention makes the invention comprehensive and complete, and conveys the concept of the embodiments of this invention to those skilled in the art. Therefore, other embodiments obtained by those skilled in the art without creative effort are all within the scope of protection of this invention.

[0062] It should be noted that, in the embodiments of this disclosure, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a method or server that includes a list of elements includes not only the elements expressly described, but also other elements not expressly listed, or elements inherent to implementing the method or server. Without further limitations, an element defined by the phrase "comprising a..." does not exclude the presence of other related elements (e.g., steps in the method or units in the server, such as portions of circuitry, processors, programs, or software, etc.) in the method or server that includes that element.

[0063] For example, the cell analysis method provided in this disclosure includes a series of steps, but the cell analysis method provided in this disclosure is not limited to the steps described. Similarly, the terminal provided in this disclosure includes a series of units, but the terminal provided in this disclosure is not limited to the units explicitly described, and may also include units that need to be set up for obtaining relevant information or processing based on the information. It should be noted that in the following description, the term "one embodiment" refers to a subset of all possible embodiments, but it is understood that "one embodiment" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0064] Although digital cell analysis systems can greatly improve the efficiency of microscopic examination by automatically selecting the working area and capturing and photographing cells, the fact that all of these tasks are completed automatically by the instrument makes it impossible for laboratory technicians to intuitively establish the relationship between each digital image of a cell and its position on the blood smear. Therefore, it is also impossible to monitor the working status of the digital cell analysis system, i.e., to directly observe whether the captured cell images come from the appropriate blood smear location and whether the appearance of the blood smear is normal. However, this application proposes a technical solution to the above problems, allowing users to monitor whether the captured cell images come from the appropriate sample location (such as blood samples, blood smears, bone marrow smears, or pathological sections) and the appearance of the sample (such as blood samples, blood smears, bone marrow smears, or pathological sections). A detailed description is provided below with reference to the accompanying drawings:

[0065] Figure 1 This is a schematic diagram of an optional structure of a cell analysis device provided in one embodiment of the present invention. The cell analysis device 100 includes: a control device (not shown), a digital imaging device (not shown), an image acquisition device 101, and a display output device 102. The control device is configured to adjust the relative position of the digital imaging device and the sample; the digital imaging device (not shown) includes a lens group and a digital camera. The image acquisition device 101 is configured to acquire digital images of cells in the sample and images characterizing the appearance morphology of the sample; the display output device 102 is configured to output the digital images of the cells and images characterizing the appearance morphology of the sample. (See reference...) Figure 2 and 4 .

[0066] Specifically, in one embodiment, the sample is, for example, a blood smear; wherein, a smear is a substrate for smearing a specimen, such as a glass slide on which blood has been evenly smeared and stained with blood cells.

[0067] In one embodiment, the control device may be configured to adjust the relative position of the digital imaging device and the sample based on mechanical transmission (e.g., a stepper motor), for example, controlling the use of a low-power microscope to locate single-cell layers and cells, and then controlling the switch to a high-power microscope (e.g., 100X) to form a specified field of view in the blood smear.

[0068] In one embodiment, the digital imaging apparatus includes a lens group (typically comprising 10X, 40X, and 100X lenses; 100X imaging usually requires the application of immersion oil to the blood smear) and a digital camera. The lens group can, for example, be a microscope objective lens, and the digital camera can be one or more (e.g., two or three). Since the number of blood sample smears to be examined is usually large, an automatic placement device is also included to automatically place the smears into the imaging position of the lens group, thereby increasing the system's processing speed and reducing the workload of medical personnel.

[0069] In one embodiment, the automated placement device includes a mechanical conveyor. The mechanical conveyor can be, for example, a robotic arm for clamping the smear to a position facing the lens assembly. The mechanical conveyor can also be, for example, a belt for conveying the smear to the position facing the lens assembly.

[0070] For example, when a smear cassette containing multiple blood sample smears is placed in the receiving section, an automatic transmission device transports the smear cassette from the receiving section to the area where the lens group is located. The smears are then removed sequentially from the compartments of the smear cassette or from a designated compartment of the smear cassette and placed in the imaging position of the lens group. After the image is captured, the smear is returned to the smear cassette. Once one smear cassette has been captured, it is returned to the receiving section, and then the process continues to capture the image of the next smear cassette, achieving high-efficiency batch capture of smears.

[0071] In one embodiment, the cell analysis device 100 may further include a carrier for placing one or more smears. For example, the shape may be square, round, etc. A light-transmitting hole is provided at the position where the smear is placed to ensure the brightness of the captured image. A fixing part (e.g., a clamp) may also be provided to hold the smear to keep its position stable.

[0072] In one embodiment, the image acquisition device 101 is configured to acquire digital images of cells in a blood smear and images characterizing the appearance of the blood smear. In one embodiment, the display output device 102 is configured to output the digital images of the cells and images characterizing the appearance of the sample to a display interface, as can be referred to... Figure 2 and 4 However, the display positions of the two images can be adjusted as needed: they can be displayed side-by-side, or top-bottom, etc., and are not limited to the example in the figure. For example, the display output device can be a display output interface (i.e., an electrical interface) for outputting digital / analog image signals, capable of outputting image signals to an external display. The display output device may also include a display output interface and a display device, wherein the display device is connected to the display output interface to receive the signals output by the display output interface and correspondingly display the digital image of cells in the blood smear and an image that characterizes the appearance of the blood smear. When the display output device is implemented as a display output interface, different display devices can be configured to connect to the display output interface according to the usage environment, for example, at least two displays can be connected to enable simultaneous detection by multiple people. The display output device can output and display the digital image of cells obtained in a specified field of view of the blood smear, and can also output and display a schematic diagram that characterizes the appearance of the blood smear currently being analyzed. This allows users to observe and monitor whether the appearance of the prepared blood smear is normal while observing the obtained digital image of the cells. Users can also choose whether to use the captured cell image or to re-prepare and analyze the sample.

[0073] In one embodiment, the display output device 102 can be configured to output a digital image of the cell and an image characterizing the morphology of the sample on the same display interface, as can be referred to. Figure 2 and 4 Other display methods may also be used according to the user's needs; no specific restrictions are made here.

[0074] In one specific embodiment, digital images of the cells in the sample and images characterizing the sample's morphology can be simultaneously displayed on the same screen. This allows users to visually observe whether the sample's (e.g., a blood smear) morphology is normal while viewing the digital images of the cells. Alternatively, they can appear sequentially on the same screen, allowing users to simultaneously observe both the digital images of the cells and the images characterizing the sample's morphology. Furthermore, other display methods are possible, such as sequential display (not appearing simultaneously on the screen), or selective display based on the user's needs.

[0075] In one embodiment, the image characterizing the appearance of the sample (e.g., a blood smear) is a digital image of the blood smear taken by the digital camera, such as... Figure 2 Or it could be a simulated image that can characterize the appearance of the sample, such as Figure 4 .

[0076] Specifically, in one embodiment, the digital image of a blood smear captured by a digital camera can be a digital image of the entire blood smear, as can be referred to... Figure 2 For example, during analysis, a digital camera captures a digital image of each blood smear being analyzed, accurately reflecting the current appearance of the smear. The digital camera capturing the digital images of the blood smear (i.e., images that characterize the appearance of the blood smear) can be the same as the camera used to acquire digital images of the cells within the smear (i.e., sharing the same camera), or a different camera can be used, such as other cameras within the cell analysis equipment. For instance, a camera used to scan and identify barcodes or numbers on the blood smear when it is loaded can also be used to acquire images characterizing its appearance. Furthermore, other cameras can be set up as needed; no specific limitations are specified here.

[0077] For details, please refer to the following: Figure 3 The structure of some parts of the cell analysis equipment is described. Figure 3 This is an optional structural diagram of a cell analysis device provided in an embodiment of the present invention. The cell analysis device includes a receiving part 301 for accommodating one or more smears at a time; a smear 302, a stage 303, a first objective lens 304, a second objective lens 305, a third objective lens 306, an eyepiece 307, and a digital imaging device 308, wherein the digital imaging device 308 includes a lens group and a digital camera.

[0078] In one embodiment, the image characterizing the appearance of the blood smear is a simulated image, which can be referenced. Figure 4 When analyzing different blood smears, the same simulated image can be used; alternatively, the simulated image can be changed as needed, for example, by inputting it into the device or selecting a new simulated image within the device. For instance, blood smears can be produced by a slide pusher. For blood smears produced by the same type of slide pusher (or the same slide pusher), a simulated image can be drawn using drawing software based on the general appearance of the produced blood smears or the appearance of a specific blood smear (such as a blood smear with a better appearance). The same simulated image can represent the appearance of all or a batch of blood smears produced. The general appearance of the produced blood smears or the appearance of a specific blood smear can be obtained through human observation or scanning by a scanning device; no specific limitation is made here. Furthermore, if the slide pusher (or its type) used to produce the blood smears changes, the simulated image can be changed or not; no specific limitation is made here.

[0079] In one embodiment, the control device is further configured to acquire digital image location information of the cells in a sample (e.g., a blood smear), and obtain information about the corresponding position in the image that characterizes the appearance of the sample (e.g., a blood smear) based on the acquisition location information.

[0080] Specifically, such as Figure 3 As shown, in one embodiment, during the analysis process, the digital imaging device 308 does not move. The cell analysis device selects a suitable shooting position by controlling the moving smear 302. The device can obtain the position information of the captured digital image of the cell in the smear (such as its coordinates in the smear), and then find its corresponding position in the image that characterizes the appearance of the blood smear based on this position information. Because the image of the appearance of the blood smear, whether captured or simulated, is proportional to the size of the blood smear being analyzed (enlarged, reduced, or unchanged), the positional relationship between the two is also corresponding.

[0081] In one embodiment, the display output device 102 is further configured to: output a digital image of the cell and an image characterizing the appearance morphology of the sample, and output the corresponding position in the image characterizing the appearance morphology of the sample. (See reference...) Figure 2 and 4 -5. As shown in the figure, the specific location of the cells in the right-side image can be marked with a box in the image of the appearance of the blood smear on the left. Figure 4-5 The middle arrow is only used to more clearly indicate the position of the box in the diagram; it will not be displayed in actual use.

[0082] In one embodiment, the display output device can output a digital image of cells obtained in a specified field of view of a blood smear, and can also output an image that characterizes the appearance of the blood smear currently being analyzed. Within this image characterizing the appearance of the blood smear, the device can output (identify) the position of the currently displayed digital image of cells in the blood smear corresponding to the location where the image was captured. (For reference...) Figure 2 and 4 This not only allows users to observe and monitor the appearance and morphology of the prepared blood smear while viewing the acquired digital cell images, but also allows users to visually observe whether the cell images were taken from the appropriate blood smear location.

[0083] In one embodiment, the display output device is further configured to output objective lens information.

[0084] Specifically, in one embodiment, outputting objective lens information includes displaying the objective lens information using at least one of the following methods: color, graphics, text, and numbers. For example, directly displaying the objective lens information using 10x or 100x resolution on the interface (such as at the edge or corner); other methods can also be used to output and display the objective lens information, which are not specifically limited here.

[0085] In one embodiment, the method of outputting the corresponding location in the image characterizing the appearance of the sample includes: identifying the corresponding location using at least one of graphics, text, and numbers (one identifier corresponds to a digital image of a cell). See also... Figure 2 and 4 As shown in the figure, a box is used to mark the location corresponding to the cell image on the right side in the image of the blood smear's appearance. In one embodiment, the size of the marker is the same as the size of the digital image of the corresponding cell; this means that the size is the same when the scale is the same. Alternatively, the size of the marker and the size of the digital image of the cell may not be the same, and this can be determined according to the actual situation; no specific limitation is made here.

[0086] In one embodiment, the display output device is further configured to output objective lens information, wherein when the corresponding position is identified using at least one of the graphics, text, and numbers, the objective lens information is represented by the color, line thickness, or solid / darkness of at least one of the graphics, text, and numbers.

[0087] In one specific embodiment, see reference. Figure 2 A box is used to mark the location of the cell digital image shown on the right in the blood smear within the image on the left that characterizes the appearance of the sample. The color of the box can also indicate the corresponding objective lens information (i.e., the objective lens used to capture the corresponding cell digital image), such as blue for 10X and red for 100X. Other methods can also be used to represent objective lens information; specific limitations are not specified here.

[0088] In one embodiment, outputting the corresponding position in the image that characterizes the appearance of the sample includes: updating and outputting the corresponding position in the image that characterizes the appearance of the sample, which can be referred to as... Figure 5-8 Alternatively, you can choose to keep the location corresponding to the previously captured shooting path; see below for reference. Figure 4-8 It allows users to monitor and observe the entire shooting process, enabling timely adjustments and controls.

[0089] Specifically, in one embodiment, during the operation, a designated field of view is formed in the blood smear using a designated lens. A digital camera typically captures multiple digital images of cells within this designated field of view, with the capture positions potentially being the same or different. If the position of the cell digital image captured by the digital camera changes, the corresponding position of the currently / latest captured cell image is updated and output in an image that characterizes the sample's morphology (the process of dynamically displaying the capture position can be found in [reference]). Figure 4-8 This allows users to observe and monitor in real time whether the currently captured cell image originated from a suitable blood smear location, facilitating subsequent image selection for analysis and other operations. Furthermore, during the dynamic display of the capture location, the position corresponding to the previous capture path can be retained and displayed for reference. Figure 4-8 You can retain the corresponding position markers, such as boxes, or display the previous, several, or all shooting positions; there are no specific limitations here. You can also choose to retain the objective lens information on the corresponding position markers, such as color, line thickness, or whether it's solid or blurred to represent objective lens information. Other methods can also be used to retain and display the objective lens information corresponding to each previous shooting position; there are no specific limitations here.

[0090] In one embodiment, if the positions corresponding to the previously captured shooting path are retained, an identifier indicating the display order of each position can be output. This identifier can be at least one of arrows, numbers, letters, colors, etc., without specific limitations. The positions along the shooting path can be displayed statically or updated dynamically. Furthermore, images displaying each position along the shooting path, or data related to each position, can be saved. Identifying the shooting path allows users to more intuitively understand and monitor the device's operating status and the shooting process, while saving the data facilitates subsequent retrieval.

[0091] In one embodiment, the control device is further configured to receive any location information in an image that characterizes the appearance of the sample, and control the display output device to output a digital image of the corresponding cell in the sample.

[0092] Specifically, in one embodiment, the control device can receive any location information selected by the user in an image that characterizes the appearance of the sample. The user can also select from location information corresponding to a previously captured path. The control device can then control the display output device to output a digital image of the cells at the corresponding location in the sample. For example, the user can select a location in an image that characterizes the appearance of the sample by clicking a mouse, using a keyboard, a touchscreen, gestures, or voice commands. The control device can then receive the user's instruction, i.e., receive the location information selected in the image that characterizes the appearance of the sample, and based on the received location information, control the display output device to output a digital image of the cells at the corresponding location in the sample. This allows the user to conveniently view the corresponding digital image of cells by selecting a suitable location on the blood smear.

[0093] In one embodiment, the cell analysis device further includes an image processing unit configured to classify the acquired digital images of the cells, such as classifying nucleated cells captured in the images. The image processing unit is optional and can be configured or not.

[0094] In one specific embodiment, the captured nucleated cells are classified; this module is optional.

[0095] Figure 9 This is a schematic diagram of a cell analysis device provided in an embodiment of the present invention. The cell analysis device includes: an image acquisition device (not shown in the figure), configured to acquire digital images of cells in a blood smear and images characterizing the appearance of the blood smear; and a display 903, configured to output the acquired digital images of cells in the blood smear and images characterizing the appearance of the blood smear. Optionally, an image processing device (not shown in the figure) is configured to classify the acquired digital images of the cells, such as classifying nucleated cells in the images. The image processing device may be one or more application-specific integrated circuits (ASICs), DSPs, programmable logic devices (PLDs), complex programmable logic devices (CPLDs), field-programmable gate arrays (FPGAs), general-purpose processors, controllers, microcontrollers (MCUs), microprocessors, or other electronic components.

[0096] Figure 10This is a schematic diagram of a cell analysis system provided in an embodiment of the present invention. The cell analysis device of the present invention is applied in this cell analysis system, which includes a cell analysis device 1001, a cell analysis device 1002, and a display device 1003. The cell analysis device 1001 includes: an image acquisition device (not shown in the figure), configured to acquire digital images of cells in a blood smear and images characterizing the appearance of the blood smear; and a display output device 1011, configured to output the digital images of cells in the blood smear and images characterizing the appearance of the blood smear. It can also output the location information of the cell digital image capture, as well as corresponding objective lens information, etc., as described in the previous embodiments and will not be repeated here.

[0097] The cell analysis device 1001 and the cell analysis device 1002 have the same structure, including: an image acquisition device (not shown in the figure) and a display output device 1012. The cell analysis system shown in this embodiment is a cluster application of the cell analysis devices disclosed in this invention, and the number of cell analysis devices and display devices is not limited by this invention.

[0098] The display device 1003 can display digital images of cells in the blood smear and images that characterize the appearance of the blood smear. Similarly, it can also display the location information of the cell digital image capture, as well as the corresponding objective lens information, etc., as described in the previous embodiments and will not be repeated here. Figure 11 This is an optional flowchart illustrating a cell analysis method provided in an embodiment of the present invention, applicable to a cell analysis device.

[0099] Step 1101: Acquire digital images of the cells in the sample and images that characterize the appearance and morphology of the sample;

[0100] Specifically, in one embodiment, the sample is, for example, a blood smear; wherein, a smear is a substrate for smearing a specimen, such as a glass slide on which blood has been evenly smeared and stained with blood cells.

[0101] In one embodiment of the method of the present invention, the digital imaging device includes a lens group and a digital camera. Since the number of blood sample smears to be examined is usually large, an automatic placement device is also provided for automatically placing the smears to the imaging position of the lens group, thereby increasing the system's processing speed and reducing the workload of medical personnel.

[0102] In one embodiment of the method of the present invention, the automatic placement device includes a mechanical conveying unit. The mechanical conveying unit can be, for example, implemented as a robotic arm for clamping the smear to a position facing the lens assembly. The mechanical conveying unit can also be, for example, implemented as a conveyor belt for conveying the smear to the position facing the lens assembly.

[0103] For example, when a smear cassette containing multiple blood sample smears is placed in the receiving section, an automatic transmission device transports the smear cassette from the receiving section to the area where the lens group is located. The smears are then removed sequentially from the compartments of the smear cassette or from a designated compartment of the smear cassette and placed in the imaging position of the lens group. After the image is captured, the smear is returned to the smear cassette. Once one smear cassette has been captured, it is returned to the receiving section, and then the process continues to capture the image of the next smear cassette, achieving high-efficiency batch capture of smears.

[0104] In one embodiment of the method of the present invention, in the cell analysis device to which the method is applied, the digital imaging device, such as a lens group, can be a microscope objective lens. The cell analysis device may also include a sample carrier for placing one or more smears, which, for example, may be square, round, etc., and a light-transmitting hole is provided at the position where the smear is placed to ensure the brightness of the captured image; a fixing part (e.g., a clamp) may also be provided for clamping the smear to keep its position stable.

[0105] For details, please refer to the following: Figure 3 The structure of some parts of the cell analysis equipment is described. Figure 3 This is an optional structural diagram of a cell analysis device provided in an embodiment of the present invention. The cell analysis device includes a receiving part 301 for accommodating one or more smears at a time; a smear 302, a stage 303, a first objective lens 304, a second objective lens 305, a third objective lens 306, an eyepiece 307, and a digital imaging device 308, wherein the digital imaging device 308 includes a lens group and a digital camera.

[0106] Step 1102: Output digital images of the cells in the sample and images that characterize the appearance and morphology of the sample. (Refer to...) Figure 2 and 4 .

[0107] Specifically, in one embodiment, a digital image of cells obtained in a specified field of view of the blood smear can be output and displayed, along with a schematic diagram representing the morphology of the blood smear currently being analyzed. This allows users to observe and monitor the morphology of the prepared blood smear while viewing the obtained digital cell image. Users can also choose whether to use the captured cell image or to redo the sample preparation and analysis.

[0108] In one embodiment of the method of the present invention, the method may further include the following steps: outputting digital images of cells in the sample and images characterizing the appearance morphology of the sample on the same display interface, as can be referred to. Figure 2 and 4 .

[0109] In one specific embodiment, digital images of the cells in the sample and images characterizing the sample's morphology can be simultaneously output on the same display interface. Alternatively, they can appear sequentially on the same display interface, allowing the user to simultaneously observe both the digital images of the cells and the images characterizing the sample's morphology. Furthermore, other display methods are possible, such as sequential display (not appearing simultaneously on the same interface), or selective display based on the user's needs.

[0110] An embodiment of the present invention provides a method for analyzing cells, which may further include: acquiring the shooting location information of a digital image of the cells in a sample, and obtaining information about the corresponding position in the image characterizing the appearance morphology of the sample based on the shooting location information. Specifically, as shown... Figure 3 As shown, in one embodiment, during the analysis process, the digital imaging device 308 does not move. The cell analysis device selects a suitable shooting position by controlling the moving smear 302. The device can obtain the position information of the captured digital image of the cell in the smear (such as its coordinates in the smear), and then find its corresponding position in the image that characterizes the appearance of the blood smear based on this position information. Because the image of the appearance of the blood smear, whether captured or simulated, is proportional to the size of the blood smear being analyzed (enlarged, reduced, or unchanged), the positional relationship between the two is also corresponding.

[0111] An embodiment of the present invention provides a method for analyzing cells, which outputs a digital image displaying cells in the sample and an image characterizing the appearance morphology of the sample, and further includes: outputting a digital image displaying cells in the sample and an image characterizing the appearance morphology of the sample, and outputting the corresponding position in the image characterizing the appearance morphology of the sample.

[0112] Specifically, in one embodiment, the output displays a digital image of cells obtained in a specified field of view of the blood smear. Simultaneously, it can also output an image characterizing the appearance of the currently analyzed blood smear, and within this image characterizing the appearance of the blood smear, it outputs (marks) the position of the currently displayed digital image of cells in the blood smear corresponding to the location where the image was captured. (Refer to...) Figure 2 and 4 This not only allows users to observe and monitor the appearance and morphology of the prepared blood smear while viewing the acquired digital cell images, but also allows them to visually observe whether the cell images were taken from the appropriate blood smear location.

[0113] Figure 12 This is an optional flowchart illustrating a cell analysis method provided in an embodiment of the present invention, applicable to a cell analysis device.

[0114] 1201: Acquire digital images of cells in the sample and images that characterize the appearance and morphology of the sample;

[0115] 1202: Obtain the location information of the digital image of the cell in the sample, and obtain the information of the corresponding position in the image that can characterize the appearance morphology of the sample based on the location information;

[0116] 1203: Outputs and displays digital images of the cells in the sample and images that characterize the appearance of the sample, and outputs and displays the corresponding positions in the images that characterize the appearance of the sample.

[0117] The specific embodiments of steps 1201-1203 can be referred to the description of the same steps above, and will not be repeated here.

[0118] This embodiment allows users to conveniently observe the acquired digital images of cells, the morphology of the currently analyzed blood smear, and whether the cell images were taken from a suitable blood smear location. Users can also choose whether to use the captured cell images or to re-prepare and analyze the sample.

[0119] In one embodiment, the image characterizing the appearance of the sample is a digital image captured by a digital camera, which can be referenced. Figure 2 Alternatively, it could be a simulated image that characterizes the appearance of the sample, for reference. Figure 4 .

[0120] Specifically, in one embodiment, the digital image of the blood smear captured by the digital camera can be a digital image of the entire blood smear; for example, during analysis, the digital camera captures a digital image of each blood smear being analyzed, which can realistically reflect the appearance of the current blood smear, as can be referenced. Figure 2 The digital camera used to capture digital images of blood smears (i.e., images that characterize the appearance and morphology of blood smears) can be the same as the digital camera used to acquire digital images of cells in the blood smear (i.e., sharing the same camera), or a different camera can be used, such as other cameras in cell analysis equipment, or cameras used to scan and identify barcodes or numbers on blood smears when they are loaded. These can also be used to acquire images that characterize the appearance and morphology of blood smears. Furthermore, other cameras can be set up as needed, or third-party cameras can be used for shooting, etc., without specific limitations here.

[0121] In one embodiment, the image characterizing the appearance of the blood smear is a simulated image, which can be referenced. Figure 4When analyzing different blood smears, the same simulated image can be used; alternatively, the simulated image can be changed as needed, for example, by inputting it into the device or selecting it within the device. For instance, blood smears can be produced by a slide pusher. For blood smears produced by the same type of slide pusher (or the same slide pusher), a simulated image can be drawn using drawing software based on the general appearance of the produced blood smears or the appearance of a specific blood smear (such as a blood smear with a better appearance). The same simulated image can represent the appearance of all or a batch of blood smears produced. The general appearance of the produced blood smears or the appearance of a specific blood smear can be obtained through human observation or scanning; no specific limitation is made here. Furthermore, if the slide pusher (or its type) used to produce the blood smears changes, the simulated image can be changed or not; no specific limitation is made here.

[0122] In one embodiment of the method of the present invention, the method further includes outputting and displaying objective lens information.

[0123] Specifically, in one embodiment, outputting objective lens information includes displaying the objective lens information using at least one of the following methods: color, graphics, text, and numbers. For example, directly displaying the objective lens information using 10x or 100x resolution on the interface (such as at the edge or corner); other methods can also be used to output and display the objective lens information, which are not specifically limited here.

[0124] In one embodiment of the method of the present invention, the method of outputting the corresponding position in the image characterizing the appearance of the sample includes: identifying the corresponding position using at least one of graphics, text, and numbers (one identifier corresponds to one digital image of a cell). See also... Figure 2 As shown in the left image of the blood smear's appearance, a box is used to mark the location of the digital image of the cells on the right side within the blood smear. In one embodiment, the size of the marker is the same as the size of the digital image of the corresponding cell; this means that the size is the same when the scale is the same. Alternatively, the size of the marker and the size of the digital image of the cell may differ, depending on the specific circumstances, and no specific limitation is made here.

[0125] In one embodiment of the method of the present invention, the method further includes outputting and displaying objective lens information, wherein when the corresponding position is identified by at least one of the graphics, text and numbers, the objective lens information is represented by the color, line thickness or solid / darkness of at least one of the graphics, text and numbers.

[0126] In one specific embodiment, see reference. Figure 2A box is used to mark the location of the cell digital image shown on the right in the blood smear within an image that characterizes the appearance of the sample. The color of the box indicates the corresponding objective lens information (i.e., the objective lens used to capture the corresponding cell digital image), such as blue for 10X and red for 100X. Other methods can also be used to represent objective lens information; specific limitations are not specified here.

[0127] In one embodiment of the method of the present invention, the method further includes, in which the output displays a digital image of the cells in the sample and an image characterizing the appearance of the sample, the method comprises: updating the output of the corresponding position in the image characterizing the appearance of the sample, which can be referenced. Figure 5-8 For example, you can use a box to mark the new shooting location; alternatively, you can choose to keep the location corresponding to the previous shooting path, as shown in the reference. Figure 6-8 The box markers corresponding to the locations along each shooting path can be retained and displayed. This allows users to monitor and observe the entire shooting process, enabling timely adjustments and controls. Furthermore, digital images of cells in the sample, images characterizing the sample's morphology, images showing the sample's morphology at the locations corresponding to the shooting path, or data at the locations corresponding to the shooting path can be saved for later review.

[0128] Specifically, in one embodiment, during the operation, a designated field of view is formed in the blood smear using a designated lens. A digital camera typically captures multiple digital images of cells within this designated field of view, with the capture positions potentially being the same or different. If the position of the cell digital image captured by the digital camera changes, the corresponding position of the currently / latest captured cell image is updated and output in an image that characterizes the sample's morphology (the process of dynamically displaying the capture position can be found in [reference]). Figure 5-8 This allows users to observe and monitor in real time whether the currently captured cell image originated from a suitable blood smear location, facilitating subsequent image selection for analysis and other operations. Furthermore, during the dynamic display of the capture location, the position corresponding to the previous capture path can be retained and displayed for reference. Figure 6-8 This can be used to display the previous, several, or all shooting locations, etc., without specific limitations.

[0129] In one embodiment of the method of the present invention, the method further includes, if the positions corresponding to the previously captured paths are retained, outputting an identifier indicating the display order of each corresponding position. The identifier can be at least one of arrows, numbers, letters, colors, etc., and is not specifically limited here. The positions of each location on the captured path can be displayed statically or updated dynamically. Furthermore, the images displaying each position on the captured path, or the data of each position on the captured path, can be saved. Identifying the captured path allows users to more intuitively understand and monitor the working status of the device, the capturing process, etc., while saving the data facilitates subsequent retrieval.

[0130] In one embodiment of the method of the present invention, the method further includes receiving any location information selected by the user in an image that can characterize the appearance and morphology of the sample. The user can also select from location information corresponding to a previously captured path. Based on the location information, a digital image of the cells in the corresponding sample is output and displayed. This allows the user to conveniently view the corresponding cell digital image by selecting a suitable location on the blood smear.

[0131] Figure 13 This is an optional structural schematic diagram of the cell analysis device provided in an embodiment of the present invention. Figure 13 The illustrated cell analysis device 1300 includes at least one processor 1301, a memory 1302, at least one network interface 1304, and a user interface 1303. The various components in the cell analysis device 1300 are coupled together via a bus system 1305. It is understood that the bus system 1305 is used to implement communication between these components. In addition to a data bus, the bus system 1305 also includes a power bus, a control bus, and a status signal bus. However, for clarity, ... Figure 13 The general labeled all buses as Bus System 1305.

[0132] The user interface 1303 may include a monitor, keyboard, mouse, trackball, click wheel, buttons, touchpad, or touch screen.

[0133] It is understood that memory 1302 can be volatile memory or non-volatile memory, or both. Non-volatile memory can be read-only memory (ROM), programmable read-only memory (PROM), erasable programmable read-only memory (EPROM), electrically erasable programmable read-only memory (EEPROM), ferromagnetic random access memory (FRAM), flash memory, magnetic surface memory, optical disc, or compact disc read-only memory (CD-ROM); magnetic surface memory can be disk storage or magnetic tape storage. Volatile memory can be random access memory (RAM), which is used as an external cache. By way of example, but not limitation, many forms of RAM are available, such as Static Random Access Memory (SRAM), Synchronous Static Random Access Memory (SSRAM), Dynamic Random Access Memory (DRAM), Synchronous Dynamic Random Access Memory (SDRAM), Double Data Rate Synchronous Dynamic Random Access Memory (DDRSDRAM), Enhanced Synchronous Dynamic Random Access Memory (ESDRAM), SyncLink Dynamic Random Access Memory (SLDRAM), and Direct Rambus Random Access Memory (DRRAM).The memory 1302 described in this embodiment of the invention is intended to include these and any other suitable types of memory.

[0134] The memory 1302 in this embodiment includes, but is not limited to, a tri-state content-addressable memory and a static random access memory capable of storing various types of data, such as received cell images, to support the operation of the cell analysis device 800. Examples of such data include: any computer program used to operate on the cell analysis device 1300, such as an operating system 1306 and an application program 1307, storing image data, classification information, etc. The operating system 1306 includes various system programs, such as a framework layer, a core library layer, and a driver layer, used to implement various basic services and handle hardware-based tasks. The application program 1307 may include various applications, such as a client or application with cell analysis functionality, used to implement various application services including: acquiring digital images of cells in a sample and images that characterize the appearance of the sample; acquiring the shooting position information of the digital images of the cells in the sample, and obtaining information about the corresponding position in the image characterizing the appearance of the sample based on the shooting position information; outputting and displaying the digital images of the cells in the sample and the image characterizing the appearance of the sample, and outputting and displaying the corresponding position in the image characterizing the appearance of the sample, etc. The program implementing the corresponding operation of any of the method embodiments described above can be included in the application program 1307.

[0135] The methods disclosed in the above embodiments of the present invention can be implemented by a processor 1301. The processor 1301 can be an integrated circuit chip with signal processing capabilities. During implementation, each step of the above method can be completed through integrated logic circuits in the hardware of the processor 1301 or through software operations. The processor 1301 can be a general-purpose processor, a digital signal processor (DSP), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The processor 1301 can implement or execute the methods, steps, and logic block diagrams disclosed in the embodiments of the present invention. The general-purpose processor can be a microprocessor or any conventional processor, etc. The steps of the methods disclosed in the embodiments of the present invention can be directly manifested as execution by a hardware decoding processor, or execution by a combination of hardware and software modules in the decoding processor. The software modules can be located in a storage medium, which is located in memory 1302. The processor 1301 reads information from memory 1302 and, in conjunction with its hardware, completes the aforementioned corresponding steps.

[0136] In an exemplary embodiment, the present invention also provides a computer-readable storage medium, such as a memory 1302 including a computer program, which can be executed by the processor 1301 of the cell analysis device 1300 to complete the various steps of the aforementioned method. The computer-readable storage medium may be a memory such as FRAM, ROM, PROM, EPROM, EEPROM, Flash Memory, magnetic surface memory, optical disc, or CD-ROM; or it may be various devices including one or any combination of the above-mentioned memories, such as a portable analyzer.

[0137] This invention also provides a computer-readable storage medium storing a computer program that, when executed by a processor, performs the following: adjusting the relative position of the digital imaging device and the smear based on mechanical transmission; acquiring digital images of cells in the sample and images that characterize the appearance of the sample; outputting and displaying digital images of cells in the sample and images that characterize the appearance of the sample; optionally, outputting digital images of cells in the sample and images that characterize the appearance of the sample on the same display interface.

[0138] In one embodiment, a computer-readable storage medium stores a computer program that, when executed by a processor, performs the following: adjusting the relative position of the digital imaging device and the smear based on mechanical transmission; acquiring digital images of cells in the sample and images characterizing the appearance of the sample; acquiring the shooting position information of the digital images of the cells in the sample, and obtaining information about the corresponding position in the images characterizing the appearance of the sample based on the shooting position information; outputting and displaying the digital images of the cells in the sample and the images characterizing the appearance of the sample, and outputting and displaying the corresponding position in the images characterizing the appearance of the sample.

[0139] In one embodiment, a computer-readable storage medium stores a computer program that, when executed by a processor, performs the steps of any of the method embodiments described above.

[0140] Those skilled in the art will understand that embodiments of the present invention can be provided as methods, systems, or computer program products. Therefore, embodiments of the present invention can take the form of hardware embodiments, software embodiments, or embodiments combining software and hardware aspects. Furthermore, embodiments of the present invention can take the form of computer program products implemented on one or more computer-usable storage media (including disk storage and optical storage, etc.) containing computer-usable program code.

[0141] Embodiments of the present invention are described with reference to flowchart illustrations and / or block diagrams of methods, apparatus (systems), and computer program products according to embodiments of the invention. It will be understood that each block of the flowchart illustrations and / or block diagrams, and combinations of blocks in the flowchart illustrations and / or block diagrams, can be implemented by computer program operations. These computer programs can be provided to operate on a processor of a general-purpose computer, special-purpose computer, embedded processor, or other programmable data processing device to produce a machine, such that operations performed by the processor of the computer or other programmable data processing device produce implementations in the flowchart. Figure 1 One or more processes and / or boxes Figure 1 A device that provides the functions specified in one or more boxes.

[0142] These computer program operations may also be stored in a computer-readable storage medium that can direct a computer or other programmable data processing device to operate in a particular manner, such that the operations stored in the computer-readable storage medium produce an article of manufacture including an operating device, the operating device being implemented in a process Figure 1 One or more processes and / or boxes Figure 1 The function specified in one or more boxes.

[0143] These computer program operations can also be loaded onto a computer or other programmable data processing equipment, causing a series of operational steps to be performed on the computer or other programmable equipment to produce a computer-implemented process, thereby providing the operations performed on the computer or other programmable equipment for implementing the process. Figure 1 One or more processes and / or boxes Figure 1 The steps of the function specified in one or more boxes.

[0144] The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A cell analysis device, the cell analysis device comprising: A control device configured to adjust the relative position of the digital imaging device and the sample; Digital imaging apparatus, including lens group and digital camera; An image acquisition device is configured to acquire digital images of cells in a sample and images that characterize the appearance and morphology of the sample. The control device is further configured to acquire the shooting position information of the digital image of the cell, and obtain information about the corresponding position in the image that can characterize the appearance morphology of the sample based on the shooting position information; The display output device is configured to output digital images of the cells and images that characterize the appearance and morphology of the sample on the same display interface.

2. The device according to claim 1, characterized in that: Digital cameras that capture images that characterize the appearance of a sample include cameras that capture images of samples by scanning and recognizing barcodes or numbers.

3. The device according to claim 1, characterized in that: The image that can characterize the appearance of the sample is a digital image taken by the digital camera, or a simulated image that can characterize the appearance of the sample.

4. The device according to claim 1, characterized in that: The display output device is further configured to: output a digital image of the cell and an image that characterizes the appearance of the sample, and output the corresponding position in the image that characterizes the appearance of the sample.

5. The device according to claim 4, characterized in that: The lens assembly includes the objective lens of the microscope; the display output device is further configured to output objective lens information, which is information about the objective lens used when taking digital images of the cells.

6. The device according to claim 5, characterized in that: The output objective information includes displaying objective information using at least one of color, graphics, text, and numbers, or displaying objective information in the image that characterizes the appearance of the sample.

7. The device according to claim 4, characterized in that: The method of outputting the corresponding position in the image that can characterize the appearance of the sample includes: identifying the corresponding position using at least one of graphics, text, and numbers.

8. The device according to claim 7, characterized in that: The display output device is further configured to output objective lens information, and when the corresponding position is identified using at least one of the graphics, text, and numbers, the objective lens information is represented by the color, line thickness, or solid / darkness of at least one of the graphics, text, and numbers.

9. The device according to claim 4, characterized in that: The step of outputting the corresponding position in the image that can characterize the appearance of the sample includes: if the position of the digital image of the cell changes, then updating and outputting the corresponding position of the current digital image of the cell in the image that can characterize the appearance of the sample; and retaining the position corresponding to the previous shooting path.

10. The device according to claim 9, characterized in that: If the positions corresponding to the previous shooting paths are retained, the corresponding positions will be displayed in the order indicated.

11. The device according to claim 10, characterized in that: The identifier is at least one of arrows, numbers, and letters.

12. The device according to claim 1, characterized in that: The control device is also configured to receive any position information in an image that can characterize the appearance of the sample, and based on the received position information, control the display output device to output a digital image of the cell at the corresponding position in the sample.

13. A method for analyzing cells, applied to a cell analysis device, the method comprising: Acquire digital images of cells in the sample and images that characterize the appearance and morphology of the sample; The digital image of the cell is obtained from the location information of the cell in the sample, and the information of the corresponding position in the image that can characterize the appearance of the sample is obtained based on the location information. The digital image of the cells in the sample and the image that characterizes the appearance of the sample are displayed on the same display interface, and the corresponding position is displayed in the image that characterizes the appearance of the sample.

14. The method according to claim 13, characterized in that: Digital cameras that capture images that characterize the appearance of a sample include cameras that capture images of samples by scanning and recognizing barcodes or numbers.

15. The method according to claim 13, characterized in that: The image that can characterize the appearance of the sample is a digital image taken by a digital camera, or a simulated image that can characterize the appearance of the sample.

16. The method according to claim 13, characterized in that: It also includes outputting objective lens information, which is the information of the objective lens used when capturing digital images of the cells.

17. The method according to claim 16, characterized in that: The output display objective information includes at least one of color, graphics, text and numbers, or displaying objective information in the image that characterizes the appearance of the sample.

18. The method according to claim 13, characterized in that: The method of outputting the corresponding position in the image that can characterize the appearance of the sample includes: identifying the corresponding position using at least one of graphics, text, and numbers.

19. The method according to claim 18, characterized in that: It also includes outputting and displaying objective lens information, and when the corresponding position is identified by at least one of the graphics, text, and numbers, the objective lens information is represented by the color, line thickness, or solid / darkness of at least one of the graphics, text, and numbers.

20. The method according to claim 13, characterized in that: The output displays digital images of cells in the sample and images that characterize the appearance of the sample, including: if the position of the digital image of the cell changes, updating the corresponding position of the current digital image of the cell in the image that characterizes the appearance of the sample; and retaining the corresponding position of the previously captured path.

21. The method according to claim 20, characterized in that: If the positions corresponding to the previously captured shooting path are retained, an identifier indicating the order in which each position is displayed will be output.

22. The method according to claim 21, characterized in that: The identifier is at least one of arrows, numbers, and letters.

23. The method according to claim 13, characterized in that: It also includes receiving any location information in an image that can characterize the appearance of a sample, and outputting a digital image of the cell at the corresponding location in the sample based on the received location information.

24. A method for analyzing cells, applied to a cell analysis device, the method comprising: Acquire digital images of cells in the sample and images that characterize the appearance and morphology of the sample; The digital image of the cell is obtained from the location information of the cell in the sample, and the information of the corresponding position in the image that can characterize the appearance of the sample is obtained based on the location information. The output displays digital images of the cells in the sample and images that characterize the appearance of the sample; the digital images of the cells in the sample and images that characterize the appearance of the sample are also output on the same display interface.

25. The method according to claim 24, characterized in that: Digital cameras that capture images that characterize the appearance of a sample include cameras that capture images of samples by scanning and recognizing barcodes or numbers.

26. The method according to claim 24, characterized in that: The output displaying digital images of cells in the sample and images that characterize the appearance of the sample further includes: outputting digital images of cells in the sample and images that characterize the appearance of the sample, and outputting the corresponding position in the image that characterizes the appearance of the sample.

27. A cell analysis device, the cell analysis device comprising: Memory, configured to store executable instructions; When a processor is configured to execute executable instructions stored in the memory, it performs the method of analyzing cells as described in any one of claims 13 to 26.

28. A computer-readable storage medium storing executable instructions configured to cause a processor to execute the executable instructions to implement the method of analyzing cells according to any one of claims 13 to 26.

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