Fully automatic flow cytometer

Abstract

The present invention discloses a fully automatic flow cytometer. The fully automatic flow cytometer includes a test and analysis system, and the test and analysis system includes a sample loading tray, a sample loading needle, a forward detection board, an electrical compartment, a WDM module, a flow cell seat, a laser, and a liquid path compartment. The sample loading needle is arranged at the upper right of the sample loading tray. The forward detection board is arranged on the right side of the sample loading needle. The electrical compartment is arranged at the lower right of the forward detection board. The WDM module is arranged on the right side of the forward detection board. The flow cell seat is arranged above the forward detection board. The laser is arranged on the left side of the flow cell seat. The liquid path compartment is arranged below the laser. The fully automatic flow cytometer of the present invention can realize the full automation of the entire process from sample pretreatment to output of the test report, can greatly reduce the test time consumption and eliminate the differences in manual consistency, and greatly improve the test accuracy.

Description

Technical Field

[0001] The present invention relates to a fully automatic flow cytometer, and particularly to a highly efficient and stable fully automatic flow cytometer. Background Art

[0002] At present, single-tube manual loading flow cytometers are mainly available on the market. The analysis software is relatively professional and requires trained personnel to operate and analyze. At the same time, complex sample pretreatment needs to be carried out manually before manual loading for detection, and manual gating analysis is also required.

[0003] With the popularization of human immune index detection (various immunodeficiency diseases) and cytokine (early cancer screening and typing), the number of flow cytometry samples shows an increasing trend, as well as the trend of full automation in clinical testing. If pure manual sample preparation continues to be used, large-scale sample preparation is impossible. As the operation time increases, the error rate rises, and the consistency of sample processing cannot be fully guaranteed, and the sample processing rate is low. At the same time, manual transfer of flow tubes by personnel to the centrifuge poses a certain safety hazard to the operators. At the same time, with the popularization of fully automatic clinical testing instruments (one-key result output) in the market, the demand for fully automatic flow cytometers by end-users is urgent. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem of low efficiency and instability of flow cytometers.

[0005] To achieve the above object of the invention, the present invention provides a fully automatic flow cytometer.

[0006] The fully automatic flow cytometer includes a test and analysis system, and the test and analysis system includes:

[0007] A sample loading tray;

[0008] A sample loading needle, arranged at the upper right of the sample loading tray;

[0009] A front detection plate, arranged on the right side of the sample loading needle;

[0010] An electrical compartment, arranged at the lower right of the front detection plate;

[0011] A WDM module, arranged on the right side of the front detection plate;

[0012] A flow cell holder, arranged above the front detection plate;

[0013] A laser, arranged on the left side of the flow cell holder; and

[0014] A liquid path compartment, arranged below the laser.

[0015] As an alternative technical solution, the sample loading tray includes a barcode scanning device.

[0016] As an alternative technical solution, the sample loading tray includes a mixing device.

[0017] As an alternative technical solution, the sample loading needle includes an anti-collision needle mechanism.

[0018] As an alternative technical solution, the WDM module is a beam splitting module.

[0019] As an alternative technical solution, the test and analysis system further includes a lens adjustment mechanism, and the lens adjustment mechanism adjusts the axial position of the lens barrel.

[0020] As an alternative technical solution, the fully automatic flow cytometer further includes a flow sample pretreatment system, and the flow sample pretreatment system includes:

[0021] A frame module;

[0022] A sample arm module, movably mounted on the frame module;

[0023] A sample rack module, arranged below the sample arm module, for accommodating a plurality of sample containers and driving the plurality of sample containers to flip;

[0024] A sample puncture module, movably mounted on the sample arm module, and the sample puncture module includes a sample needle for puncturing the sample container;

[0025] A reagent arm module, movably mounted on the frame module and parallel to the sample arm module;

[0026] A reagent rack module, arranged below the reagent arm module, for accommodating at least one reagent container;

[0027] A reagent needle module, movably mounted on the reagent arm module, and the reagent needle module includes a reagent needle for adding the reagent in the reagent container to the sample container to form a secondary sample container;

[0028] A centrifuge module, arranged beside the sample rack module and / or the reagent rack module, for accommodating the secondary sample container and performing centrifugation on the secondary sample container;

[0029] A clamping module, movably mounted on the reagent arm module, for grasping and transferring the secondary sample container;

[0030] An oscillating vortex module, arranged beside the centrifuge module, for accommodating the secondary sample container and performing oscillation on the secondary sample container; and

[0031] A control module for providing control and / or operation to all components of the flow sample pretreatment system to automate the flow sample pretreatment process.

[0032] As an alternative technical solution, the sample rack module includes:

[0033] A sample rack for holding a plurality of the sample containers;

[0034] A gear connected to one end of the sample rack; and

[0035] A first motor disposed below the gear to drive the gear to drive the sample rack to flip.

[0036] As an alternative technical solution, the sample puncture module further includes:

[0037] A holding portion;

[0038] A second motor for driving the holding portion to move to hold the sample container; and

[0039] A third motor for driving the sample needle to puncture the sample container when the holding portion holds the sample container.

[0040] As an alternative technical solution, the centrifuge module includes:

[0041] A centrifuge including a cover and an angle rotor, and a plurality of container jacks for inserting the second-generation sample containers are provided on the annular surface of the angle rotor;

[0042] A thickened chassis for carrying the centrifuge; and

[0043] An imbalance protection module for gradually reducing the speed when the centrifuge reaches the imbalance critical point.

[0044] Compared with the prior art, the full-automatic flow cytometer of the present invention can realize automatic sample information entry, automatic generation of the process, and automatic output of the detection report after data analysis. There is no need for the user to perform any operations during the processing process. It can realize the full automation of the entire process from sample pretreatment to output of the detection report, which can greatly reduce the test time-consuming and eliminate the differences in manual consistency, greatly improve the test accuracy, make the error in sample processing zero, and truly realize one-key reporting, fundamentally shortening the time from when the patient finishes drawing blood to obtaining the report. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The present invention will be further described below in conjunction with the drawings and embodiments.

[0046] Figure 1 It is a top view of the full-automatic flow cytometer according to an embodiment of the present invention.

[0047] Figure 2 is Figure 1 a schematic diagram of another perspective of a fully automatic flow cytometer.

[0048] Figure 3 is Figure 1 a schematic diagram of another perspective of a fully automatic flow cytometer.

[0049] Figure 4 is Figure 1 a schematic diagram of the sample rack module in the flow sample pretreatment system.

[0050] Figure 5 is Figure 1 a schematic diagram of the first state of the sample puncture module in the flow sample pretreatment system.

[0051] Figure 6 is Figure 5 a schematic diagram of another perspective of the sample puncture module.

[0052] Figure 7 is Figure 1 a schematic diagram of the second state of the sample puncture module in the flow sample pretreatment system.

[0053] Figure 8 is Figure 7 a schematic diagram of another perspective of the sample puncture module.

[0054] Figure 9 is Figure 1 a schematic diagram of the third state of the sample puncture module in the flow sample pretreatment system.

[0055] Figure 10 is Figure 9 a schematic diagram of another perspective of the sample puncture module.

[0056] Figure 11 is Figure 1 a schematic diagram of the cover of the centrifuge in the flow sample pretreatment system.

[0057] Figure 12 is Figure 1 a schematic diagram of the angle rotor of the centrifuge in the flow sample pretreatment system.

[0058] Figure 13 is Figure 1 a partial schematic diagram of the angle rotor of the centrifuge in the flow sample pretreatment system.

[0059] Figure 14 is Figure 1 another partial schematic diagram of the angle rotor of the centrifuge in the flow sample pretreatment system.

[0060] Figure 15 isFigure 1 Schematic diagram of the clamping module and reagent needle module in the flow sample pretreatment system.

[0061] Figure 16 is Figure 1 Schematic diagram of the clamping module in the flow sample pretreatment system.

[0062] Figure 17 is Figure 1 Partial schematic diagram of the reagent needle module in the flow sample pretreatment system.

[0063] Figure 18 is Figure 1 Schematic diagram of the oscillation vortex module in the flow sample pretreatment system.

[0064] Figure 19 is Figure 1 Partial sectional schematic diagram of the oscillation vortex module in the flow sample pretreatment system.

[0065] Figure 20 is along Figure 19 Partial sectional schematic diagram of the dividing line AA.

[0066] Figure 21 is Figure 20 Simplified schematic diagram. Specific implementation manner

[0067] Next, in combination with the accompanying drawings and specific implementation manners, the present invention will be further described. It should be noted that, on the premise of no conflict, any combination of the following-described embodiments or technical features can form a new embodiment.

[0068] Figure 1 is the top view of the full-automatic flow cytometer in this embodiment, Figure 2 is Figure 1 Schematic diagram of another perspective of the full-automatic flow cytometer, Figure 3 is Figure 1 Schematic diagram of another perspective of the full-automatic flow cytometer. Please refer to Figures 1 to 3 .

[0069] The full-automatic flow cytometer includes a full-automatic flow sample pretreatment system 100 and a full-automatic test and analysis system 200.

[0070] Next, the structure and working principle of the flow sample pretreatment system 100 will be described in detail.

[0071] As Figures 1 to 3As shown in the figure, the flow sample pretreatment system 100 includes a frame module 11, a sample arm module 1, a sample rack module 4, a sample puncture module 3, a reagent arm module 2, a reagent rack module 7, a reagent needle module 16, a centrifuge module 6, a clamping module 9, a shaking and vortexing module 8, and a control module. Among them, the sample arm module 1 is movably mounted on the frame module 11. The sample rack module 4 is disposed below the sample arm module 1 and is used to accommodate a plurality of sample containers 21 and drive the plurality of sample containers 21 to flip. The sample puncture module 3 is movably mounted on the sample arm module 1. The sample puncture module 3 includes a sample needle 22, and the sample needle 22 is used to puncture the sample container 21. The reagent arm module 2 is also movably mounted on the frame module 11 and is parallel to the sample arm module 1. The reagent rack module 7 is disposed below the reagent arm module 2 and is used to accommodate at least one reagent container 23. The reagent needle module 16 is movably mounted on the reagent arm module 2. The reagent needle module 16 includes a reagent needle 24, and the reagent needle 24 is used to add the reagent in the reagent container 23 into the sample container 21 to form a second-generation sample container 25. In addition, the clamping module 9 is also movably mounted on the reagent arm module 2 and is used to grab and transfer the second-generation sample container 25.

[0072] The centrifuge module 6 is disposed beside the sample rack module 4 and / or the reagent rack module 7 and is used to accommodate the second-generation sample container 25 and perform centrifugation on the second-generation sample container 25. The shaking and vortexing module 8 is disposed beside the centrifuge module 6 and is used to accommodate the second-generation sample container 25 and perform shaking on the second-generation sample container 25. The control module is used to provide control and / or operation for all components of the flow sample pretreatment system 100 of the present invention to realize the full automation of the flow sample pretreatment process.

[0073] As described above, the flow sample pretreatment system 100 of the present invention realizes the full-automation of the flow sample pretreatment process through automatic sample and reagent transfer, and automatically performs processes such as mixing, centrifugation, and shaking, greatly improving the test efficiency, reducing the test error, and having high safety at the same time.

[0074] In this embodiment, the frame module 11 adopts a gantry structure, thus avoiding the deformation of the arm group when the load of the cantilever structure of the arm group is large and ensuring the service life of the X-direction guide rail of the arm group. Preferably, a slide rail structure is provided on one cantilever of the frame module 11 for the sliding of the sample arm module 1 and the reagent arm module 2. At the same time, a slide rail is also provided on the sample arm module 1 for the sliding of the sample puncture module 3, and a slide rail is also provided on the reagent arm module 2 for the sliding of the reagent needle module 16 and the clamping module 9. This is because the slide rail structure is simple and convenient for movement, but the present invention is not limited thereto, and other ways that can achieve movable installation are also applicable.

[0075] The flow sample pretreatment system 100 of the present invention further includes a needle washing pool module. The needle washing pool module includes a sample needle washing pool 5 and a reagent needle washing pool 15. Among them, the sample needle washing pool 5 is used to clean the sample needle 22, and the reagent needle washing pool 15 is used to clean the reagent needle 24. The two needle washing pools can be set nearby in the corresponding activity areas. For example, the sample needle washing pool 5 is set beside the centrifuge module 6, and the reagent needle washing pool 15 is set beside the oscillating vortex module 8. Setting two separate needle washing pools avoids cross-contamination caused by mixed cleaning of the sample needle 22 and the reagent needle 24.

[0076] Next, each module will be elaborated in detail with reference to the accompanying drawings.

[0077] As Figure 4 shown, the sample rack module 4 includes a sample rack 28, a gear 29, and a first motor 30. Among them, the sample rack 28 is used to hold a plurality of sample containers 21. The gear 29 is connected to one end of the sample rack 28, and the first motor 30 is arranged below the gear 29 to drive the gear 29 to drive the entire sample rack 28 to flip. Compared with the time-consuming disadvantage of the single-tube sequential shaking of the traditional U-shaped sample rack, the sample rack 28 of the present invention adopts an overall shaking type sample rack, which is time-saving and efficient. In a better embodiment, the gear 29 adopts POM gear transmission, reducing the rotation abnormal noise.

[0078] In addition, the flow sample pretreatment system 100 further includes an identification module 14. The identification module 14 is arranged at the other end of the sample rack module 4 and is used to read the barcodes on the sample container 21 and / or the second-generation sample container 25. In other words, when the user loads different containers, the identification module 14 automatically reads the barcodes on the containers and identifies the sample information, saving the time of manually entering the samples, thus realizing the automatic sample information entry of the flow sample pretreatment system 100. Preferably, the identification module 14 is a barcode scanner.

[0079] Please continue to refer to Figures 5 to 10 , the sample puncture module 3 further includes a holding part 32, a second motor 33, and a third motor 34. The second motor 33 is used to drive the holding part 32 to move to hold the sample container 21, and the third motor 34 is used to drive the sample needle 22 to puncture the sample container 21 when the holding part 32 holds the sample container 21. Among them, the second motor 33 and the third motor 34 are arranged in parallel, the moving directions of the holding part 32 and the sample needle 22 are the same, and the sample needle 22 is arranged directly above the holding part 32. Specifically, as Figure 5 and Figure 6 shown, when performing sample puncture, the sample puncture module 3 moves above the sample container 21 to be punctured so that the holding part 32 aligns with the nozzle of the sample container 21; as Figure 7 and Figure 8 shown, the second motor 33 drives the holding part 32 to move down to hold the nozzle of the sample container 21; at this time, asFigure 9 and Figure 10 As shown in Figure 10 , the third motor 34 drives the sample needle 22 to move downward to pierce the sample container 21. That is to say, the holding design in the sample piercing module 3 of this embodiment not only avoids the offset of the sample container 21 during piercing, but also ensures that the sample needle 22 will not bend when stressed. At the same time, the holding and piercing adopt a dual-motor drive method to ensure that the sample needle 22 will not bring out the sample container 21 during the pushing-back process after piercing.

[0080] Please refer to Figures 11 to 14 , the centrifuge module 6 includes a centrifuge 35, a thickened chassis 36 and an imbalance protection module. The thickened chassis 36 is used to carry the centrifuge 35, ensuring the stability of the centrifuge 35 during operation. The addition of the imbalance protection module ensures that the centrifuge 35 gradually decelerates when reaching the imbalance critical point, further protecting the service life of the centrifuge 35 and the safety of relevant operators. The centrifuge 35 includes a cover 37 and an angle rotor 38. The cover 37 is provided with an openable and closable cover 39 and a motor for driving the opening and closing of the cover 39. A plurality of container jacks 40 for inserting a plurality of second-generation sample containers 25 are provided on the annular surface of the angle rotor 38, and the plurality of container jacks 40 are hinged and fixed to form a ring. The centrifuge 35 of the present invention adopts a self-switching angle rotor. When the centrifuge 35 reaches a certain rotational speed, the centrifuge 35 automatically switches to the angle rotor 38. After centrifugation, due to the self-gravity of the container holder, it returns to the vertical state, so as to facilitate the transfer of the second-generation sample container 25 in the centrifuge 35 by the clamping module 9. The unique centrifuge balancing algorithm of the whole machine ensures that the centrifugation experiment can be carried out smoothly in the case of an odd number of samples.

[0081] Please refer to Figure 15 and Figure 16 , the clamping module 9 includes a plurality of clamping jaws 41 and a fourth motor 42. The plurality of clamping jaws 41 surround to form an automatically adjustable grasping space for grasping the second-generation sample container 25. The fourth motor 42 is used to drive the plurality of clamping jaws 41 to move up and down, left and right, front and back and rotate. Specifically, the clamping jaw 41 is a torsion automatically adjustable 360-degree self-rotating electric clamping jaw that can grasp different reaction containers, such as flow tubes, EP tubes, and vacuum blood collection tubes. At the same time, the grasping force is adjusted according to the different containers grasped, and it can automatically rotate after grasping the container, so as to facilitate the identification module 14 to perform barcode scanning. Please refer to Figure 15 and Figure 17 , the reagent needle module 16 further includes a fifth motor 43. The fifth motor 43 is used to drive the reagent needle 24 to add the reagent in the reagent container 23 to the sample container 21 to form the second-generation sample container 25. As Figure 17As shown, in this embodiment, the reagent needle module 16 includes two reagent needles and corresponding two motors, and can be designed by appropriately considering the reagent addition efficiency and type differences, which is not limited in the present invention. Preferably, the clamping module 9 and the reagent needle module 16 are connected as a whole, for example, are respectively arranged on both sides of the reagent arm module 2 and move simultaneously.

[0082] Please refer to Figures 18 to 21 , the oscillation vortex module 8 includes an oscillation device 44, a clamping device 45 and an oscillation vortex motor. The oscillation vortex motor is used to drive the oscillation device 44 to perform oscillation vortex on the second-generation sample container 25, and to drive the clamping device 45 to clamp and release. Specifically, the clamping device 45 includes a chassis 46, a collar 47 and an annular gasket 48. The collar 47 is movably hinged to the chassis 46 through a hinge 49. A spring 50 can be designed at the end of the hinge 49 for example. The annular gasket 48 is fixed on the collar 47 so that the second-generation sample container 25 passes through the annular gasket 48 and the collar 47 in sequence. When a plurality of jaws 41 transfer the second-generation sample container 25 into the collar 47, the oscillation vortex motor drives the collar 47 to rotate to drive the annular gasket 48 to contract, thereby clamping and releasing the second-generation sample container 25. The oscillation vortex module 8 of this embodiment uses a single motor to drive oscillation vortex and has an automatic clamping function. The whole module can be pulled out to facilitate the user to replace consumables.

[0083] In this way, through the series of fully automatic processes of sample reagent transfer, steel needle cleaning, automatic mixing, oscillation, and centrifugation of the above-mentioned flow sample pretreatment system 100, the pretreatment of the sample is realized.

[0084] That is, the flow sample pretreatment system of the present invention includes a frame module, a sample arm module, a sample rack module, a sample puncture module, a reagent arm module, a reagent rack module, a reagent needle module, a centrifuge module, a clamping module, an oscillation vortex module and a control module. The sample puncture module is used to puncture the sample container. The reagent needle module is used to add the reagent in the reagent container to the sample container to form a second-generation sample container. The centrifuge module performs centrifugation processing. The oscillation vortex module performs oscillation processing. The control module is used to provide control and / or operation for all components of the flow sample pretreatment system of the present invention. In this way, through automatic sample reagent transfer and automatic mixing, centrifugation, oscillation and other processes, a fully automatic flow sample pretreatment process is realized, greatly improving the test efficiency, reducing the test error, and having high safety at the same time.

[0085] Next, the automatic analysis and test process of the sample will be specifically described to realize the full automation of the entire system.

[0086] Specifically, as Figures 1 to 3As shown, the test analysis system 200 includes a sample loading tray 13 , a sample loading needle 17 , a front-end detection board 18 , an electrical compartment 19 , a WDM module 52 , a flow cell seat 26 , a laser 27 and a liquid path compartment 51 .

[0087] The sample loading needle 17 is arranged at the upper right of the sample loading plate 13, the front detection plate 18 is arranged at the right side of the sample loading needle 17, the electric compartment 19 is arranged at the lower right side of the front detection plate 18, the WDM module 52 is arranged at the right side of the front detection plate 18, the flow cell seat 26 is arranged above the front detection plate 18, the laser 27 is arranged on the left side of the flow cell seat 26, and the liquid circuit compartment 51 is arranged below the laser 27. Among them, the liquid circuit adopts a plunger pump to load the sample, which further ensures the accuracy of sample and reagent transfer.

[0088] That is, after automatic sampling, multiple projects can be tested in parallel and automated test analysis can be performed.

[0089] In this way, the fully automatic flow cytometer of the present invention can realize automatic sample information input, automatic process generation, and automatic output of test reports after data analysis. The user does not need to perform any operations during the processing, and the entire process from sample pre-processing to output of test reports can be fully automated, which can greatly reduce the test time and eliminate differences in manual consistency, greatly improve the accuracy of the test, and make the error in sample processing zero. It truly realizes one-click report generation, fundamentally shortening the time from blood drawing to obtaining the report for patients.

[0090] The sample loading tray 13 includes a barcode scanning device 12, which can scan and record the information of the sample in real time.

[0091] In addition, the sample is mixed before loading, that is, the loading plate 13 has a mixing device.

[0092] Among them, the sample loading needle 17 is provided with an anti-collision needle mechanism, which can fully ensure the stability of sample loading.

[0093] The WDM module 52 is a light splitting module, which can greatly reduce the attenuation of the fluorescent signal caused by multiple reflections, making the light splitting module more compact.

[0094] Among them, the limiting design of the flow cell seat 26 and its lens adjustment mechanism can eliminate the radial runout and circumferential rotation of the lens barrel, and has high stability. At the same time, the lens adjustment mechanism itself can realize the axial position adjustment of the lens barrel to ensure that the center of the flow cell and the center of the focusing lens are on the same axis.

[0095] In summary, the fully automatic flow cytometer of the present invention can realize automatic sample information input, automatic process generation, and automatic output of test reports after data analysis. The user does not need to perform any operations during the processing, and the entire process from sample pre-processing to output of test reports can be fully automated, which can greatly reduce the test time and eliminate differences in manual consistency, greatly improve the accuracy of the test, and make the error in sample processing zero. It truly realizes one-click report generation, fundamentally shortening the time from blood drawing to obtaining the report for patients.

[0096] It should be understood that although this specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each implementation mode may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

[0097] The present invention is explained from the viewpoints of purpose of use, efficiency, progress and novelty, and its practical progress meets the functional enhancement and use requirements emphasized by the Patent Law. The above description and drawings of the present invention are only preferred embodiments of the present invention, and are not intended to limit the present invention. Therefore, all structures, devices, features, etc. that are similar or identical to the present invention, that is, all equivalent replacements or modifications made according to the scope of the patent application of the present invention, should fall within the scope of protection of the patent application of the present invention.

Claims

1. An automatic flow cytometer, characterized in that, the automatic flow cytometer includes a test and analysis system, and the test and analysis system includes: A sample loading tray; A sample loading needle, arranged at the upper right of the sample loading tray; A front detection board, arranged on the right side of the sample loading needle; An electrical compartment, arranged at the lower right of the front detection board; A WDM module, arranged on the right side of the front detection board; A flow cell seat, arranged above the front detection board; A laser, arranged on the left side of the flow cell seat; and A liquid path compartment, arranged below the laser; The automatic flow cytometer further includes a flow sample pretreatment system, and the flow sample pretreatment system includes: A frame module; A sample arm module, movably installed on the frame module; A sample rack module, used to accommodate a plurality of sample containers and drive the plurality of sample containers to flip; A sample puncture module, movably installed on the sample arm module, and the sample puncture module includes a sample needle, and the sample needle is used to puncture the sample container; A reagent arm module, movably installed on the frame module and parallel to the sample arm module; A reagent rack module, used to accommodate at least one reagent container; A reagent needle module, movably installed on the reagent arm module, and the reagent needle module includes a reagent needle, and the reagent needle is used to add the reagent in the reagent container to the sample container to form a second-generation sample container; A centrifuge module, used to accommodate the second-generation sample container and perform centrifugation on the second-generation sample container; A clamping module, movably installed on the reagent arm module, used to grab and transfer the second-generation sample container; An oscillating vortex module, used to accommodate the second-generation sample container and perform oscillation on the second-generation sample container; and A control module, used to provide control and / or operation for all components of the flow sample pretreatment system to realize the automation of the flow sample pretreatment process.

2. The automatic flow cytometer according to claim 1, characterized in that, the sample loading tray includes a barcode scanning device.

3. The automatic flow cytometer according to claim 1, characterized in that, the sample loading tray includes a mixing device.

4. The automatic flow cytometer according to claim 1, characterized in that, the sample loading needle includes an anti-collision needle mechanism.

5. The automatic flow cytometer according to claim 1, characterized in that, the WDM module is a beam splitting module.

6. The automatic flow cytometer according to claim 1, characterized in that, the test and analysis system further includes a lens adjustment mechanism, and the lens adjustment mechanism adjusts the axial position of the lens barrel.

7. The automatic flow cytometer according to claim 1, characterized in that, the sample rack module is arranged below the sample arm module; the reagent rack module is arranged below the reagent arm module; a slide rail structure is arranged on one cantilever of the frame module for the sample arm module and the reagent arm module to slide; the centrifuge module is arranged beside the sample rack module and / or the reagent rack module; the oscillating vortex module is arranged beside the centrifuge module.

8. The full-automatic flow cytometer according to claim 7, characterized in that, the sample rack module includes: a sample rack for holding a plurality of the sample containers; a gear connected to one end of the sample rack; and a first motor disposed below the gear to drive the gear to drive the sample rack to turn over.

9. The full-automatic flow cytometer according to claim 7, characterized in that, the sample puncture module further includes: a holding part; a second motor for driving the holding part to move to hold the sample container; and a third motor for driving the sample needle to puncture the sample container when the holding part holds the sample container.

10. The full-automatic flow cytometer according to claim 7, characterized in that, the centrifuge module includes: a centrifuge including a cover body and an angle rotor, and a plurality of container jacks for inserting the second-generation sample containers are provided on the annular surface of the angle rotor; a thickened chassis for carrying the centrifuge; and an imbalance protection module for gradually reducing the speed when the centrifuge reaches the imbalance critical point.

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