A method for extracting plant hydrosols using dual adjuvants

Abstract

The present invention discloses a method for extracting plant hydrosol containing double auxiliary agents, which is characterized in that aromatic plants are used as raw materials, an extract made of double auxiliary agents and water for extraction is stirred evenly, infiltrated, and then distilled to collect the hydrosol. The present invention does not use a deep eutectic preparation method, but directly puts material A and material B into the water phase to form an extract containing double auxiliary agents, thereby simplifying the engineering steps and improving the extraction efficiency. Compared with the prior art, the present invention uses a green and environmentally friendly deep eutectic solvent combined with traditional distillation technology, which is not only simple to operate and low in cost, but also improves the quality of the hydrosol and the efficiency of the hydrosol extraction.

Description

Technical Field

[0001] The invention relates to a method for improving the extraction efficiency of hydrosol by combining double auxiliary agents with a traditional hydrosol extraction method, and belongs to the technical field of chemical extraction. Background Art

[0002] Hydrosol, generally refers to the byproducts produced when the leaves, flowers, bark, rhizomes, seeds and peels of aromatic plants are distilled, which are rich in water-soluble components and trace essential oils. The hydrosols of various plants have different effects, such as antibacterial, anti-inflammatory, antioxidant, whitening and other effects. Rose hydrosol, also known as rose water essential oil, is a byproduct of essential oil. The freshly picked rose flowers are put into a distillation tank and made after oil-water separation. Rose hydrosol is dissolved in water and has the effects of replenishing water, moisturizing, rapid anti-inflammatory, anti-allergic, antipruritic, and delaying aging. Chinese patent application number CN105326691A discloses a spray configured with wormwood hydrosol, which has a broad spectrum of bactericidal, anti-allergic and anti-inflammatory and antipruritic effects, and also has the effects of slowing down skin aging, promoting skin blood circulation, replenishing water, moisturizing, and refreshing. Chinese patent application publication number CN103735466A discloses a mouthwash prepared from galangal hydrosol, which has the effects of deodorizing and inhibiting bacteria, aromatic refreshing, and anti-vomiting. Hydrosols are loved by more and more consumers due to their many benefits and are widely used in daily chemical products, food, medicine, fragrance and other products. Distillation is a commonly used separation technology using water as a medium. It is very necessary to improve the extraction efficiency of hydrosols.

[0003] Deep eutectic solvent is a new type of solvent prepared by heating hydrogen bond donors and hydrogen bond acceptors, which is characterized by a melting point significantly lower than the melting point of any of its components. Deep eutectic solvents have strong intermolecular forces with the extraction raw materials, which improves the efficiency of extraction. Deep eutectic solvent literature (J.Agric.Food Chem.2017,65,3591-3601; J.Nat.Prod.2018,81,679-690; ACS Sustainable Chem.Eng.2020,8,10591-10612;) published the preparation and extraction of deep eutectics, in which the preparation of deep eutectics is a prerequisite for extraction. Patent CN111171095A discloses a method of extracting flavonoid disaccharide carbonyl glycosides from war bones by forming deep eutectics with choline chloride and any one selected from 1,3-propylene glycol, levulinic acid, glycolic acid and DL-lactic acid in a molar ratio of 1:1-3. CN111116443A discloses a method for extracting carotenoids by mixing hydrogen bond acceptors (choline chloride, DL-menthol) and hydrogen bond donors (tartaric acid, lactic acid, gluconic acid) in a certain proportion at 80-90°C to prepare a natural deep eutectic solvent. The preparation of a deep eutectic solvent is a prerequisite. Summary of the invention

[0004] The technical problems to be solved by the present invention are: when deep eutectic solvent is used for extraction, the amount of deep eutectic used is large and the deep eutectic needs to be prepared in advance.

[0005] In order to solve the above technical problems, the present invention provides a dual auxiliary agent for extraction, characterized in that it includes component A and component B, the component A includes at least one of choline chloride and betaine, and the component B includes at least one of natural acids, amines, alcohols and sugars.

[0006] Preferably, the natural acid is at least one of citric acid, malic acid, tartaric acid, malonic acid, mucic acid and amino acids derived from natural acids; the amine is at least one of organic amines such as urea and methylurea; the alcohol is at least one of ethylene glycol, 1,4-butanediol, sorbitol and glycerol; the sugar is at least one of monosaccharides, disaccharides and polysaccharides.

[0007] More preferably, the monosaccharide is at least one of maltose and sorbitol.

[0008] Preferably, the mass ratio of component A to component B is 1:0.1-10.

[0009] More preferably, the mass ratio of component A to component B is 1:0.5-5.

[0010] The present invention also provides a method for extracting plant hydrosol containing double auxiliary agents, which is characterized in that aromatic plants are used as raw materials, the extract made of the double auxiliary agents used for extraction and water is stirred evenly, infiltrated, and then distilled to collect the hydrosol.

[0011] Preferably, the aromatic plant is selected from any one or more of its flowers, leaves, stems and roots.

[0012] Preferably, the dual auxiliary agent is diluted with deionized water and stirred evenly to prepare an extract.

[0013] More preferably, the mass ratio of the dual auxiliary agent to the extract is 0.1-1000:100.

[0014] Preferably, the distillation is carried out by enzyme-assisted distillation, ultrasound-assisted distillation, ohmic-assisted heating lamp distillation method, supercritical carbon dioxide extraction or subcritical water extraction.

[0015] The present invention avoids using a deep eutectic preparation method, but directly puts material A and material B into the aqueous phase respectively to form an extraction solution with double auxiliary agents, thereby simplifying the engineering steps and improving the extraction efficiency.

[0016] Compared with the existing technology, the present invention combines green and environmentally friendly deep eutectic solvent with traditional distillation technology, which is not only simple to operate and low in cost, but also improves the quality of hydrosol and the efficiency of hydrosol extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the GC-MS ion chromatogram of the pure dew obtained by using daylily flowers as raw materials and double-auxiliary distillation extraction.

[0018] Figure 2 This is the GC-MS ion chromatogram of the hydrosol obtained using daylily flowers as raw materials using conventional distillation method. DETAILED DESCRIPTION

[0019] In order to make the present invention more clearly understood, preferred embodiments are described in detail below with reference to the accompanying drawings.

[0020] Example 1

[0021] The formula of the two-component hydrosol extraction aid (A+B) is shown in Table 1.

[0022] Table 1

[0023]

[0024]

[0025] Example 2

[0026] A method for extracting plant hydrosol (taking daylily as an example), comprising the following steps:

[0027] (1) Take 100 g of daylily flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0028] (2) According to the ratio of betaine to malic acid of 1:2, 33.3 g of betaine and 66.6 g of malic acid were mixed in a beaker.

[0029] (3) Add 500 g of deionized water into a beaker and stir evenly to obtain an extract.

[0030] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of daylily flowers and perform ultrasonic treatment for 30 minutes.

[0031] (5) Distill for 3 hours and collect the daylily hydrosol.

[0032] Comparing this embodiment with the hydrosol obtained by conventional distillation, the ion flux Figure 1 , 2 Analysis shows that Figure 1 The quality of the detected fractions increased, and the ingredients contained richer varieties of substances with higher contents.

[0033] Example 3

[0034] A method for extracting plant hydrosol (taking daylily as an example), comprising the following steps:

[0035] (1) Take 100 g of daylily flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0036] (2) Mix 66.6 g of betaine and 33.3 g of citric acid in a beaker.

[0037] (3) Add 500 g of deionized water into a beaker and stir well.

[0038] (4) Add 100 g of daylily flowers and sonicate for 30 min.

[0039] (5) Distill for 3 hours and collect the daylily hydrosol.

[0040] Example 4

[0041] A method for extracting plant hydrosol (taking daylily as an example), comprising the following steps:

[0042] (1) Take 100 g of daylily flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0043] (2) Mix 16.6 g of betaine and 83.4 g of citric acid in a beaker.

[0044] (3) Add 500 g of deionized water into a beaker and stir well.

[0045] (4) Add 100 g of daylily flowers and sonicate for 30 min.

[0046] (5) Distill for 3 hours and collect the daylily hydrosol.

[0047] Example 5

[0048] A method for extracting plant hydrosol (taking daylily as an example), comprising the following steps:

[0049] (1) Take 100 g of daylily flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0050] (2) Take 1.66 g of betaine and 8.34 g of citric acid and mix them in a beaker.

[0051] (3) Add 500 g of deionized water into a beaker and stir well.

[0052] (4) Add 100 g of daylily flowers and sonicate for 30 min.

[0053] (5) Distill for 3 hours and collect the daylily hydrosol.

[0054] Example 6

[0055] A method for extracting plant hydrosol (taking daylily as an example), comprising the following steps:

[0056] (1) Take 100 g of daylily flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0057] (2) Mix 33.3 g of betaine and 66.6 g of tartaric acid in a beaker.

[0058] (3) Add 500 g of deionized water into a beaker and stir well.

[0059] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of daylily and perform ultrasonic treatment for 30 minutes.

[0060] (5) Distill for 3 hours and collect the daylily hydrosol.

[0061] Example 7

[0062] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0063] (1) Take 100 g of rose flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0064] (2) Mix 33.3 g of betaine and 66.6 g of citric acid in a beaker.

[0065] (3) Add 500 g of deionized water into a beaker and stir well.

[0066] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment for 30 minutes.

[0067] (5) Distill for 3 hours and collect the rose water.

[0068] Example 8

[0069] A method for extracting plant hydrosol (taking jasmine as an example), comprises the following steps:

[0070] (1) Take 100 g of jasmine flowers, crush them using a blender, and add the mixture to a 2 L flask.

[0071] (2) Mix 33.3 g of betaine and 66.6 g of citric acid in a beaker.

[0072] (3) Add 500 g of deionized water into a beaker and stir well.

[0073] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of jasmine flowers and perform ultrasonic treatment.

[0074] (5) Distill for 3 hours and collect the jasmine pure water.

[0075] Example 9

[0076] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0077] (1) Take 100 g of rose flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0078] (2) Mix 33.3 g of choline chloride and 66.6 g of citric acid in a beaker.

[0079] (3) Add 500 g of deionized water into a beaker and stir well.

[0080] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment.

[0081] (5) Distill for 3 hours and collect the rose water.

[0082] Example 10

[0083] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0084] (1) Take 100 g of rose flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0085] (2) Take 20g of choline chloride and 80g of urea and mix them in a beaker.

[0086] (3) Add 500 g of deionized water into a beaker and stir well.

[0087] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment.

[0088] (5) Distill for 3 hours and collect the rose water.

[0089] Embodiment 11

[0090] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0091] (1) Take 100 g of rose flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0092] (2) According to the mass ratio of choline chloride to sorbitol of 0.5:5, 9.1 g of choline chloride and 90.9 g of sorbitol were mixed in a beaker.

[0093] (3) Add 500g of deionized water to the beaker in a ratio of 1:50 of eutectic solvent and deionized water and stir evenly.

[0094] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment.

[0095] (5) Distill for 3 hours and collect the rose water.

[0096] Example 12

[0097] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0098] (1) Take 100 g of rose flowers, grind them using a blender, and add the mixture to a 2 L flask.

[0099] (2) Take 0.91 g of choline chloride and 9.09 g of maltodextrin and mix them in a beaker.

[0100] (3) Add 500 g of deionized water into a beaker and stir well.

[0101] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment.

[0102] (5) Distill for 3 hours and collect the rose water.

[0103] Embodiment 13

[0104] A method for extracting plant hydrosol (taking rose as an example), comprises the following steps:

[0105] (1) Add water to the dried rose flower raw material, the weight of the water being 5-50% of the weight of the dried rose flower. Use a blender to grind the raw material, weigh 100 g of the ground material, and add it to a 2 L flask.

[0106] (2) Take 0.91 g of choline chloride and 9.09 g of maltodextrin and mix them in a beaker.

[0107] (3) Add 500 g of deionized water into a beaker and stir well.

[0108] (4) Pour the obtained extract containing the dual adjuvants into a flask containing 100 g of rose flowers and perform ultrasonic treatment.

[0109] (5) Distill for 3 hours and collect the rose water.

Claims

1. A method for extracting plant hydrosols containing double adjuvants, characterized in that: Aromatic plants are used as raw materials, and an extract made of a dual auxiliary agent for extraction and water is stirred evenly, infiltrated, and then distilled to collect the pure dew; the dual auxiliary agent for extraction comprises a component A and a component B, the component A comprises at least one of choline chloride and betaine, and the component B comprises at least one of natural acids, amines, alcohols and sugars; the natural acid is at least one of citric acid, malic acid, tartaric acid, malonic acid, mucic acid and amino acids; the amine is at least one of urea and methylurea; the alcohol is at least one of ethylene glycol, 1,4-butanediol, sorbitol and glycerol; the sugar is at least one of monosaccharide, disaccharide and polysaccharide, and the monosaccharide is at least one of maltose and sorbitol; the mass ratio of the component A to the component B is 1:0.1-10; the dual auxiliary agent is diluted with deionized water, stirred evenly to prepare an extract; the mass ratio of the dual auxiliary agent to the extract is 0.1-1000:

100.

2. The method for extracting plant hydrosols containing double adjuvants as claimed in claim 1, characterized in that: The mass ratio of component A to component B is 1: 0.5-5.

3. The method for extracting plant hydrosols containing double adjuvants as claimed in claim 1, characterized in that: The aromatic plant is selected from any one or more of its flowers, leaves, stems and roots.

4. The method for extracting plant hydrosols containing double adjuvants as claimed in claim 1, characterized in that: The aromatic plant is daylily, rose or jasmine.

5. The method for extracting plant hydrosols containing double adjuvants as claimed in claim 1, characterized in that: The distillation adopts enzyme-assisted distillation, ultrasound-assisted distillation, ohm-assisted heating lamp distillation method, supercritical carbon dioxide extraction or subcritical water extraction.

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