A handheld LAMP detector and system

Abstract

The present invention discloses a handheld LAMP detector and a handheld LAMP detection system. The handheld LAMP detector includes: a housing, formed with an inner cavity and provided with a chip port for inserting a LAMP chip; a chip holder, disposed in the inner cavity, for accommodating the LAMP chip; a heating assembly, disposed in the inner cavity, for heating the LAMP chip at a constant temperature; an optical detection device, disposed in the inner cavity so as to be movable in the left-right direction, the optical detection device having multiple detection positions, each corresponding to a plurality of reaction chambers on the LAMP chip; a driving mechanism, disposed in the inner cavity, for driving the optical detection device to move in the left-right direction; a battery, for powering the device; a touch screen, for displaying the detection results; a printer, for printing the detection results; and a barcode reader, for reading barcode information. The present invention integrates nucleic acid amplification and detection, automatic barcode scanning, and displaying and printing the detection results, and can be handheld for detection, making it easy to use.

Description

Technical Field

[0001] The invention belongs to the technical field of LAMP detection and relates to a handheld LAMP detector and a system. Background Art

[0002] LAMP (loop-mediated isothermal amplification) technology is widely used in the field of biodiagnosis due to its mild reaction conditions (low reaction temperature) and short reaction time. For example, it is used in nucleic acid amplification tests to diagnose the presence of pathogens in samples. LAMP technology provides conditions for in vitro amplification of nucleic acid fragments, allowing them to amplify exponentially. Fluorescent dyes or fluorescent markers are added during the amplification process, and the intensity of the fluorescent signal is detected using an optical device. The amplification results are then analyzed to determine the nucleic acid amplification results. During the nucleic acid amplification reaction, the reaction system must be heated. Current LAMP detectors integrate nucleic acid amplification and detection. When a detection chip (typically a microfluidic chip) is placed in the LAMP detector, the chip's reaction chamber (typically the amplification reaction chamber) can be heated, illuminated, and tested. One of the goals of LAMP detector designers is to make LAMP detectors compact and portable. Summary of the Invention

[0003] The invention provides a handheld LAMP detector and a handheld LAMP detection system.

[0004] According to a first aspect of the present invention, a handheld LAMP detector comprises:

[0005] A housing having an inner cavity and a chip port for inserting a LAMP chip;

[0006] a chip seat disposed in the inner cavity, the chip seat having an accommodation space for accommodating the LAMP chip, the accommodation space being opposite to the chip port;

[0007] A heating component, which is used to heat the LAMP chip at a constant temperature, and the heating component is arranged in the inner cavity;

[0008] an optical detection device, which is movably disposed in the inner cavity in a left-right direction, and has a plurality of detection positions, each of which is used to correspond to a plurality of reaction chambers on the LAMP chip;

[0009] a driving mechanism, configured to drive the optical detection device to move in a left-right direction, the driving mechanism being disposed in the inner cavity;

[0010] a battery, used to power the heating assembly, the optical detection device, and the driving mechanism, the battery being disposed in the inner cavity;

[0011] A touch screen, used for displaying the detection result, wherein the touch screen is provided on the upper side wall of the housing and is located above the battery;

[0012] A printer, used for printing the test results, wherein the printer is embedded in the upper side wall of the housing and located above the battery;

[0013] A code reader is used to read barcode information, wherein the barcode information includes sample information and / or user information. The code reader is embedded in the front side wall of the housing.

[0014] In one embodiment, the chip port is arranged on the front side wall of the shell, the chip seat is located at the front of the inner cavity, the battery is located on the rear side of the inner cavity, the heating component is arranged on the chip seat, at least part of the optical detection device is located above the chip seat, and the driving mechanism is arranged between the chip seat and the battery.

[0015] In one embodiment, the chip holder includes a fixing portion and a supporting portion located behind the fixing portion. The accommodating space includes a slot provided on the fixing portion for receiving the LAMP chip. The slot extends through the fixing portion in a front-to-back direction. The supporting portion includes a supporting surface facing the rear of the LAMP chip, and the heating assembly is disposed on the supporting surface. More preferably, the supporting surface faces upward, and / or the slot faces the chip port.

[0016] In one embodiment, the heating component includes a heat conducting plate, a heating film, thermal insulation cotton, a temperature sensor and a fuse. The thermal insulation cotton is arranged on the chip seat, the heating film is arranged on the thermal insulation cotton, the heat conducting plate is arranged on the heating film, and the temperature sensor and the fuse are embedded in the thermal insulation cotton.

[0017] In one embodiment, the driving mechanism includes a guide rail extending in the left-right direction and arranged on the inner wall of the shell, a mounting block movably arranged on the guide rail in the left-right direction, and a motor for driving the mounting block to move in the left-right direction, the motor is arranged on the inner wall of the shell, and the optical detection device is arranged on the mounting block.

[0018] In one embodiment, the battery is a rechargeable battery, and a power interface electrically connected to the battery is provided on the rear side of the housing.

[0019] In one embodiment, the handheld LAMP detector further includes a controller, and the controller is electrically connected to the optical detection device and the printer respectively.

[0020] In one embodiment, the handheld LAMP detector further includes a controller, and the controller is electrically connected to the heating component, the optical detection device, the driving mechanism, and the touch screen respectively.

[0021] In one embodiment, the touch screen has multiple display states, and in one of the display states, the touch screen has a command input area for a user to input commands. The touch screen serves as a human-computer interaction interface.

[0022] In one embodiment, the lower portion of the housing is provided with a recessed portion for easy gripping by fingers.

[0023] According to a second aspect of the present invention, a handheld LAMP detection system includes a LAMP detector and a LAMP chip. The LAMP detector is the handheld LAMP detector described above.

[0024] In one embodiment, the LAMP chip includes a chip body, which is provided with a sample injection port, multiple reaction chambers, and multiple exhaust ports. Each reaction chamber is connected to the sample injection port via a first microchannel, and each of the first microchannels has one or more bends. Each reaction chamber is also connected to a corresponding exhaust port via a second microchannel. The bends prevent backflow of reagents within the reaction chambers. The LAMP chip can prevent the reagents in each reaction tank from interfering with each other without configuring a control valve or piston, thereby avoiding contamination. In a preferred embodiment, there is only one sample injection port, and sample only needs to be added to one sample injection port, which is convenient to use.

[0025] In a preferred embodiment, the bending portion includes an arc-shaped bending portion that is arc-shaped as a whole, and the central angle of the arc of the arc-shaped bending portion is greater than 90 degrees.

[0026] In a more preferred embodiment, the arc-shaped bending portion includes a first arc-shaped bending portion and a second arc-shaped bending portion, the center of the first arc-shaped bending portion is located on the right side thereof, and the center of the second arc-shaped bending portion is located on the left side thereof.

[0027] Furthermore, the first arc-shaped bending portions and the second arc-shaped bending portions are arranged in a staggered manner, and adjacent first arc-shaped bending portions and second arc-shaped bending portions are directly connected to each other or connected to each other through a straight channel.

[0028] In one embodiment, an overflow groove is further provided on the chip body, and the overflow groove is connected to the sample addition port or surrounds the sample addition port.

[0029] In one embodiment, a positioning ridge is provided on the left side or the right side of the chip body.

[0030] In one embodiment, the LAMP chip further includes a film disposed on the surface of the chip body and covering the exhaust port, the film allowing gas to pass through but not allowing liquid to pass through.

[0031] In one embodiment, the LAMP chip further includes a first sealing film for sealing the sample injection port and / or the exhaust port.

[0032] In one embodiment, the sample addition port and the exhaust port are arranged on the first surface of the chip body, the reaction chamber is arranged on the second surface of the chip body, and the LAMP chip also includes a second sealing film for sealing the reaction chamber, and the second sealing film is covered on the second surface.

[0033] In a preferred embodiment, a plurality of grooves are provided on the second surface of the chip body. Furthermore, the first microchannel and the second microchannel are also provided on the second surface of the chip body.

[0034] The present invention adopts the above solution and has the following advantages compared with the prior art:

[0035] The LAMP detector and detection system of the present invention have a compact structure and a small size. The overall size of the LAMP detector can be as small as 250mm×120mm×80mm. It is powered by a battery and integrates nucleic acid amplification and detection, automatic code scanning, and display and printing of detection results. It can be handheld for detection, is easy to carry and easy to use. The user can automatically complete constant temperature heating amplification, fluorescence detection analysis, and detection structure display by simply installing the LAMP chip, thereby improving detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0037] Figure 1 FIG. 1 is a three-dimensional schematic diagram of a LAMP detection system according to an embodiment of the present invention at one viewing angle.

[0038] Figure 2 FIG. 1 is a three-dimensional schematic diagram of a LAMP detection system according to an embodiment of the present invention from another perspective.

[0039] Figure 3 Schematic diagram of the internal structure of a LAMP detector according to an embodiment of the present invention at one viewing angle, wherein a LAMP chip has been inserted.

[0040] Figure 4Schematic diagram of the internal structure of the LAMP detector according to an embodiment of the present invention from another perspective, in which the LAMP chip has been inserted.

[0041] Figure 5 Schematic diagram of the LAMP chip, chip holder and heating assembly.

[0042] Figure 6 FIG. 4 is a schematic diagram of a coating on the first surface of a LAMP chip according to an embodiment of the present invention.

[0043] Figure 7 FIG. 4 is a schematic diagram of the coating on the second surface of the LAMP chip according to an embodiment of the present invention.

[0044] Figure 8 A three-dimensional schematic diagram of the chip body.

[0045] Figure 9 is a schematic diagram of the first surface of the chip body.

[0046] Figure 10 is a schematic diagram of the second surface of the chip body.

[0047] Figure 11 A side view of the chip body.

[0048] Figure 12 4 is a structural block diagram of a control system of a LAMP detector according to an embodiment of the present invention.

[0049] Figure 13 Flowchart of the control process of the LAMP detector according to an embodiment of the present invention.

[0050] in,

[0051] 1. Housing; 10. Chip port; 11. Power interface; 12. Recessed portion;

[0052] 2. Chip holder; 21. Fixing portion; 21a. Slot; 22. Support portion; 22a. Support surface;

[0053] 3. Heating assembly; 31. Heat conducting plate; 32. Heating film; 33. Temperature sensor; 34. Fuse; 35. Compression spring;

[0054] 4. Optical detection device; 41. Spectral sensor;

[0055] 5. Driving mechanism; 51. Guide rail; 52. Mounting block; 53. Motor; 54. Screw; 55. Optocoupler;

[0056] 61. Battery; 610. Battery compartment; 62. Touch screen; 63. Printer; 64. Barcode scanner;

[0057] 7. LAMP chip; 70. Chip body; 70a. First surface; 70b. Second surface; 701. Sample loading port; 702. Overflow channel; 703. Exhaust port; 704. Reaction chamber; 705. First microchannel; 705a. First curved bend; 705b. Second curved bend; 706. Second microchannel; 707. Groove; 708. Notch; 71. Covering film; 72. First sealing film; 73. Second sealing film;

[0058] 81. First control chip; 82. Second control chip. DETAILED DESCRIPTION

[0059] The preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art. It should be noted that the description of these embodiments is intended to help understand the present invention, but does not constitute a limitation of the present invention.

[0060] In the description of this application, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "inside" and "outside" indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limiting this application. The directions mentioned herein are all based on Figure 3 The arrow in the middle is for reference.

[0061] This embodiment provides a handheld LAMP detector and a handheld LAMP detection system, which are used for nucleic acid detection. Figure 1 and Figure 2 As shown, the handheld LAMP detection system includes the above-mentioned LAMP detector and LAMP chip 7. The LAMP detector includes a housing 1, in which an inner cavity for mounting internal components is formed. A chip port 10 for inserting the LAMP chip 7 is provided on the front side wall of the housing 1. The LAMP chip 7 is inserted into the LAMP detector through the chip port 10. The internal structure of the LAMP detector is shown in FIG. Figures 3 to 5 As shown, the housing 1 is not shown. Figures 3 to 5 As shown, the LAMP detector also includes a chip holder 2, a heating assembly 3, an optical detection device 4, a drive mechanism 5, a battery 61, a touch screen 62, a printer 63, a barcode scanner 64, and a control system. The chip port 10 is located on the front side wall of the housing 1, the chip holder 2 is located at the front of the inner cavity, the battery 61 is located at the rear of the inner cavity, the heating assembly 3 is located on the chip holder 2, at least part of the optical detection device 4 is located above the chip holder 2, and the drive mechanism 5 is located between the chip holder 2 and the battery 61. Figures 6 to 11The LAMP chip 7 of the handheld LAMP detection system is shown. The LAMP chip 7 has a plurality of reaction chambers 704 arranged in parallel along the left-right direction.

[0062] Combine Figures 3 to 5 As shown, the chip holder 2 is disposed in the inner cavity. The chip holder 2 has a storage space for accommodating the LAMP chip 7. The storage space is opposite the chip port 10, and the LAMP chip 7 is horizontally inserted into the storage space from the chip port 10. Specifically, the chip holder 2 includes a fixing portion 21 and a support portion 22 located behind the fixing portion 21. The chip holder 2 is integrally formed, such as by plastic integral injection molding. The above-mentioned storage space includes a slot 21a provided on the fixing portion 21 for inserting the LAMP chip 7. The slot 21a extends through the fixing portion 21 in the front-to-back direction. The support portion 22 has a support surface 22a for facing the rear of the LAMP chip 7. The heating assembly 3 is disposed on the support surface 22a. Furthermore, the slot 21a is generally horizontal and flush with the chip port 10, with the two facing each other. The support surface 22a is specifically located on the upper surface of the support portion 22, and the heating assembly 3 is disposed on the support surface 22a. After the LAMP chip 7 is inserted, the rear portion of the LAMP chip 7 (i.e., the portion containing the reaction chamber 704) is located on the heating assembly 3, and the two are in contact or close proximity to each other, ideally in contact, to facilitate heating the system within the reaction chamber 704 of the LAMP chip 7 and perform constant temperature amplification. The chip holder 2 is fixed to the lower side wall of the front portion of the housing 1.

[0063] Combine Figures 3 to 5 As shown, the heating component 3 is used to heat the LAMP chip 7 at a constant temperature. The heating component 3 is arranged in the inner cavity of the shell 1 and further arranged on the supporting surface 22a of the chip holder 2. Specifically, as Figure 5 As shown, the heating component 3 includes a heat conducting plate 31, a heating film 32, thermal insulation cotton, a temperature sensor 33 and a fuse 34. The thermal insulation cotton is arranged on the support surface 22a of the chip holder 2, the heating film 32 is arranged on the thermal insulation cotton, the heat conducting plate 31 is arranged on the heating film 32, and the temperature sensor 33 and the fuse 34 are embedded in the thermal insulation cotton. Furthermore, the heat conducting plate 31, the heating film 32 and the thermal insulation cotton are stacked from top to bottom on the support surface 22a of the chip holder 2. The fuse 34 and the heating film 32 are connected in series for overcurrent protection. The heating component 3 also includes a compression spring 35 for pressing the LAMP chip 7 on the heat conducting plate 31. The compression spring 35 is fixedly arranged on the mounting seat. There are multiple compression springs 35 and they are arranged at intervals in the left and right directions. The compression spring 35 can be elastically deformed. The clamping spring 35 is generally in a U-shape with the opening facing upward. After the LAMP chip 7 is inserted, the clamping spring 35 is squeezed by the LAMP chip 7 and bends upward and parallel. Under the action of the elastic force, the lower end of the clamping spring 35 presses against the LAMP chip 7 to press it against the heat conducting plate 31, so that the two fit together, achieving a better heating effect.

[0064] Combine Figure 3 and Figure 4 As shown, the optical detection device 4 can be arranged in the inner cavity so as to be movable in the left-right direction. The optical detection device 4 has a plurality of detection positions, and the plurality of detection positions are used to respectively correspond to the plurality of reaction chambers 704 on the LAMP chip 7. During detection, the optical detection device 4 moves to a detection position and detects a corresponding reaction chamber 704; after the detection is completed, the optical detection device 4 continues to move to the next detection position and detects another corresponding reaction chamber 704. The optical detection device 4 adopts a known optical device, which can emit a laser beam to irradiate the system in the reaction chamber 704 to excite fluorescence, and can also collect the excited fluorescence and perform spectral analysis. The optical detection device 4 of this embodiment preferably adopts a monochromatic laser light source; the optical detection device 4 also includes a spectral sensor for performing spectral analysis on the excited fluorescence.

[0065] Combine Figure 3 and Figure 4 As shown, the drive mechanism 5 is used to drive the optical detection device 4 to move in the left-right direction. The drive mechanism 5 is disposed in the inner cavity. Specifically, the drive mechanism 5 includes a guide rail 51 extending in the left-right direction and disposed on the inner wall (specifically, the lower side wall) of the housing 1, a mounting block 52 movably disposed in the left-right direction on the guide rail 51, and a motor 53 for driving the mounting block 52 to move in the left-right direction. The motor 53 is disposed on the inner wall of the housing 1, and the optical detection device 4 is disposed on the mounting block 52. Furthermore, the motor 53 drives a screw rod 54 extending in the left-right direction to rotate. The screw rod 54 is connected to the mounting block 52 via a nut. As the screw rod 54 rotates, the mounting block 52 moves in the left-right direction, thereby driving the optical detection device 4 to move left and right.

[0066] Combine Figure 3 and Figure 4 As shown, the battery 61 is arranged in the inner cavity of the housing 1, and is used to power the heating component 3, the optical detection device 4, the driving mechanism 5, the touch screen 62, the printer 63, the barcode scanner 64, the control system, etc. Specifically, the battery 61 is arranged in a battery box 61, and the battery box 61 is arranged on the inner wall of the housing 1. The battery 61 is a rechargeable battery 61, and a power interface 11 (such as Figure 2 As shown), the power interface 11 can be connected to an external power source, and the LAMP detector can also be directly powered by an external power source.

[0067] Combine Figures 1 to 4As shown, the printer 63 and the touch screen 62 are both embedded in the upper side wall of the housing 1 and located above the battery 61. The touch screen 62 is located in front of the printer 63. The printer 63 can print out information such as test results, and the touch screen 62 can display information such as test results. One function of the touch screen 62 is to display information, and another function is to serve as a human-computer interaction interface for the user to input instructions. Accordingly, the touch screen 62 has multiple display states. In one display state, the touch screen 62 has a command input area for the user to input instructions, such as a "Start Test" button; in another display state, the touch screen 62 has a test result display area for displaying the test result, such as "negative" or "positive".

[0068] Combine Figure 1 、 Figure 3 and Figure 4 As shown, the barcode scanner 64 is embedded in the front side wall of the shell 1 and is located on the right or left side of the chip holder 2. It can read the barcode on the LAMP chip 7 and obtain the scanned information. The scanned information includes sample information (sample source, etc.).

[0069] The lower part and / or the side part of the housing 1 are provided with a recessed portion 12 for easy gripping by the user's fingers, so as to conform to ergonomics.

[0070] Reference Figures 6 to 11 As shown, the LAMP chip 7 includes a chip body 70, on which a sample addition port 701, a plurality of reaction chambers 704 and a plurality of exhaust ports 703 are provided. Each reaction chamber 704 is connected to the sample addition port 701 via a first microchannel 705, and each first microchannel 705 has one or more bends. Each reaction chamber 704 is also connected to a corresponding exhaust port 703 via a second microchannel 706. The LAMP chip 7 is a miniaturized microfluidic chip that does not require any piston or valve for controlling the flow of liquid. It is compact and suitable for use in a handheld LAMP detector. The first microchannel 705 of each reaction chamber 704 is bent at one or more places to form one or more bends. Without the need for a valve or piston, it can effectively prevent the reagents in the reaction chamber 704 from flowing back out of the first microchannel 705, thereby avoiding contamination of other reaction chambers 704. Reaction chambers 704 are pre-installed with amplification reagents such as primers. The primers in different reaction chambers 704 can be the same or different to detect different pathogens. The front portion of the chip body 70, where the sample injection port 701 is located, is thicker than the remaining portions. The entire chip body 70, or at least the portion corresponding to the reaction chambers 704, is made of a material with a certain degree of transparency (transparent or translucent) to allow laser light to enter and fluorescent light to escape.

[0071] The above-mentioned bending portion includes an arc-shaped bending portion that is an entirety arc-shaped, and the center angle of the arc of the arc-shaped bending portion is greater than 90 degrees, preferably 170 to 190 degrees. Further, the arc-shaped bending portion includes a first arc-shaped bending portion 705a and a second arc-shaped bending portion 705b, the center of the first arc-shaped bending portion 705a is located on the right side thereof, and the center of the second arc-shaped bending portion 705b is located on the left side thereof. The center angles of the first arc-shaped bending portion 705a and the second arc-shaped bending portion 705b are both 180 degrees. Figure 10 As shown, the first arc-shaped bend portion 705a and the second arc-shaped bend portion 705b are arranged alternately, and the adjacent first arc-shaped bend portions 705a and second arc-shaped bend portions 705b are directly connected to each other or connected through a straight channel, that is, each first microchannel 705 has a wavy portion, which effectively prevents the primers and other reagents in the reaction chamber 704 from flowing back.

[0072] In another embodiment, the aforementioned bends include a plurality of first bends and a plurality of second bends, each first bend forming an acute angle as a whole, and each second bend forming an acute angle as a whole. The first bends and the second bends are arranged alternately, and adjacent first bends and second bends are connected directly or via a linear channel. That is, each first microchannel 705 has a zigzag portion.

[0073] The chip body 70 is generally plate-shaped and is integrally formed from plastic, such as by injection molding. The chip body 70 has a first surface 70a and a second surface 70b that are opposite to each other. In this embodiment, the first surface 70a is the upper surface of the chip body 70, and the second surface 70b is the lower surface of the chip body 70. A sample injection port 701 and an exhaust port 703 are provided on the first surface 70a of the chip body 70, while a reaction chamber 704, a first microchannel 705, and a second microchannel 706 are provided on the second surface 70b of the chip body 70.

[0074] The LAMP chip 7 also includes a film 71 disposed on the surface of the chip body 70 and covering the exhaust port 703, which allows gas to pass but does not allow liquid to pass. The LAMP chip 7 also includes a first sealing film 72 for sealing the sample port 701 and / or the exhaust port 703. After sample addition is completed, the first sealing film 72 is applied to the first surface 70a of the chip body 70 to isolate the sample port 701 and the exhaust port 703 from the outside world, and then the amplification reaction is performed to prevent contamination. The first surface 70a of the chip body 70 is also provided with an overflow groove 702 arranged around the sample port 701 to prevent contamination of the first surface 70a of the chip body 70 during sample addition, thereby preventing the first surface 70a of the chip body 70 from being affected by the seal between the first sealing film 72 and the first surface 70a.

[0075] The LAMP chip 7 also includes a second sealing film 73 for sealing the reaction chamber 704, the first microchannel 705, and the second microchannel 706. The second sealing film 73 is disposed on the second surface 70b of the chip body 70. A plurality of grooves 707 are formed on the second surface 70b of the chip body 70. These grooves 707 are specifically formed in areas of the second surface 70b where the reaction chamber 704, the first microchannel 705, and the second microchannel 706 are not located. These grooves 707 have different shapes, sizes, and locations to accommodate the shape and location of the reaction chamber, the first microchannel 705, and the second microchannel 706, and avoid these reaction chambers, the first microchannel 705, and the second microchannel 706. These grooves 707 facilitate sealing between the second sealing film 73 and the chip body 70, preventing bubbles from forming after the film is applied, and also preventing the chip body 70 from bending or deforming.

[0076] When the LAMP chip 7 leaves the factory, the covering film 71 and the second sealing film 73 are pre-attached to the appropriate positions of the chip body 70, and constant temperature amplification reagents, including primers, are pre-placed in each reaction tank; after the user adds the nucleic acid sample to be tested, the second sealing film 73 is added to cover the sample port 701 and the covering film 71, and the sample port 701 and each exhaust port 703 are sealed.

[0077] A ridge or corner notch 708 is provided on the right side of the chip body 70. Correspondingly, a positioning groove that matches the positioning ridge or a protrusion that matches the corner notch 708 is provided on the slot 21a of the chip holder 2 to prevent the LAMP chip 7 from being inserted upside down, thereby providing a fool-proof design.

[0078] The handheld LAMP detector includes a controller, which is electrically connected to the above-mentioned heating component 3, optical detection device 4, drive mechanism 5, battery 61, touch screen 62, printer 63, barcode scanner 64, etc., so that the LAMP detector can operate automatically to realize nucleic acid detection, result display, etc. Figure 12 The control block diagram of the handheld LAMP detector is shown, wherein the controller specifically includes a first control chip 81 and a second control chip 82 arranged on a PCB board. The first control chip 81 is the main control chip; the second control chip 82 is the printer 63 control chip and is used for communicating with the outside, such as through Bluetooth, WiFi, 4G or 5G.

[0079] The power interface 11 is electrically connected to the external power input port of the first control chip 81. After the power interface 11 is connected to the external power supply, the external power supply can be used to power the entire LAMP detector and charge the battery 61. The battery 61 is electrically connected to the first control chip 81. When the external power supply is not connected, the battery 61 powers the entire LAMP detector. Among them, the first control chip 81 converts the 15V voltage from the external power supply or the 12V voltage from the battery 61 and supplies it to the second control chip 82, the heating component 3, the motor 53, the touch screen 62, the barcode scanner 64, the printer 63, etc. The first control chip 81 is also used to monitor the power level of the battery 61. When the power level is less than the set threshold, an insufficient power alarm signal is sent through the touch screen 62.

[0080] The heating film 32 of the heating assembly 3 is electrically connected to a serial port of the first control chip 81 via a fuse 34. The temperature sensor 33 is also electrically connected to another serial port of the first control chip 81. The first control chip 81 receives temperature data from the temperature sensor 33, compares it with the set temperature, and uses a PID algorithm to adjust the temperature of the heating film 32 in real time to maintain the heating temperature of the heat conducting plate 31 at 65±0.5°C.

[0081] The motor 53 is electrically connected to the serial port of the first control chip 81. The serial port of the first control chip 81 is also electrically connected to the optocoupler. The optocoupler detects whether the motor 53 has rotated to the set angle to reset and limit the motor 53. The spectral sensor of the optical detection device 4 is electrically connected to the serial port of the first control chip 81 to receive the detection results. After the motor 53 is in the starting position and the set reaction time has passed, the spectral sensor analyzes and detects the fluorescence of the first reaction chamber 704 and sends the detection results to the first control chip 81; after the first reaction chamber 704 is detected, the motor 53 rotates to move the optocoupler sensor to the top of the second reaction chamber 704, reads the fluorescence of the second reaction chamber 704, and sends the reading result to the second control chip 82; and so on.

[0082] The touch screen 62 is electrically connected to the serial port 3 of the first control chip 81. The touch screen 62 can receive user instructions and send them to the first control chip 81. The first control chip 81 can send detection results, scan code information, etc. to the touch screen 62 for display.

[0083] The barcode scanner 64 is electrically connected to the serial port 2 of the first control chip 81. The barcode scanner 64 can read the barcode information of the sample and obtain information such as the source of the sample and the name of the subject; the first control chip 81 can receive the read barcode information and associate it with the test results.

[0084] The printer 63 is electrically connected to the serial port 0 of the first control chip 81, and the other serial port 0 of the first control chip 81 is electrically connected to the serial port 0 of the second control chip 82. The first control chip 81 can send the read barcode information and detection results to the printer 63 for printing.

[0085] Reference Figure 13 As shown, the control process of the handheld LAMP detector is as follows:

[0086] S100, the controller obtains the power of battery 61

[0087] The first control chip 81 detects the power level of the battery 61 and determines whether the power level is greater than or equal to a set threshold. If yes, the following steps are executed; if not, a low power alarm signal is issued through the touch screen 62 or the like.

[0088] After receiving the low battery alarm signal, the user connects to an external power source, which supplies power and charges the battery 61 .

[0089] S101, scan code

[0090] The barcode information is read by the barcode scanner 64 . The barcode information includes sample information, user information, etc. The barcode information may be a one-dimensional code or a two-dimensional code provided on the LAMP chip 7 .

[0091] S102, install LAMP chip 7

[0092] The LAMP chip 7, which has already been pre-treated, is inserted horizontally into the handheld LAMP detector through the chip port 10. The pre-treatment process is as follows: Sample is added through the sample port 701 until it flows to the cover film 71, which seals the cover film 71, even if any excess sample overflows into the overflow trough 702. A first sealing film 72 is applied to the sample port 701 and the cover film 71, respectively, sealing the sample port 701 and each exhaust port 703. This creates a sealed environment within the LAMP chip 7, isolated from the outside air, where constant temperature amplification and fluorescence detection are performed.

[0093] S103: The touch screen 62 receives a detection instruction from the user.

[0094] The touch screen 62 switches to a display state with a “Start Detection” button, and the user clicks the “Start Detection” button on the touch screen 62 to start the detection process.

[0095] S104: The controller receives the detection instruction and controls the motor 53 to reset and the heating component 3 to heat.

[0096] After receiving the user's start detection instruction, the first control chip 81 sends a control signal to the motor 53. In response to this control signal, the motor 53 resets to its starting position. The first control chip 81 specifically determines whether the motor 53 has returned to its starting position based on the signal returned by the optocoupler. At the same time, the first control chip 81 controls the heating of the heating film 32. Specifically, the first control chip 81 receives the heating temperature value fed back by the temperature sensor 33, compares this heating temperature value with the set temperature value (65°C), and controls the heating temperature of the heating film 32 to maintain at 65±0.5°C through the PID algorithm, so as to be able to maintain constant temperature heating of the reaction system in each reaction chamber 704 of the LAMP chip 7.

[0097] S105: The controller controls the optical detection device 4 to read the detection result of the first reaction chamber 704.

[0098] The first control chip 81 sends a control signal to the motor 53. In response to the control signal, the motor 53 rotates a certain angle, which is calculated based on the initial position of the optical detection device 4 and the distance between the first reaction chamber 704. Therefore, the optical detection device 4 can be moved to the first detection position facing the first reaction chamber 704 to irradiate the amplification product in the first reaction chamber 704 with a laser, collect the excited fluorescence, analyze the collected fluorescence through the spectral sensor, and send the fluorescence analysis value to the first control chip 81.

[0099] S106: The controller controls the optical detection device 4 to read the detection result of the next reaction chamber 704.

[0100] After receiving the fluorescence analysis value of the previous reaction chamber 704, the first control chip 81 sends a control signal to the first control chip 81, causing the motor 53 to continue rotating to another set angle. The angle is calculated based on the distance between two adjacent reaction chambers 704, thereby enabling the optical detection device 4 to move to the next detection position facing the next reaction chamber 704 (such as the second, third, fourth reaction chamber 704, etc.) to perform laser irradiation on the amplification product in the next reaction chamber 704, collect the excited fluorescence, analyze the collected fluorescence through the spectral sensor, and send the fluorescence analysis value to the first control chip 81.

[0101] S107: The controller determines the fluorescence analysis value sent by the optical detection device 4 and obtains the detection results of the detection items corresponding to each reaction chamber 704.

[0102] After the first control chip 81 receives the fluorescence analysis value returned by the optical detection device 4 for the first time, it determines the positive or negative of the detection item (such as pathogen A) corresponding to the first reaction chamber 704 according to the pre-stored interpretation standard. After the first control chip 81 receives the fluorescence analysis value returned by the optical detection device 4 for the second time, it determines the positive or negative of the detection item (such as pathogen B) corresponding to the second reaction chamber 704 according to the pre-stored interpretation standard. After the first control chip 81 receives the fluorescence analysis value returned by the optical detection device 4 for the third time, it determines the positive or negative of the detection item (such as pathogen C) corresponding to the third reaction chamber 704 according to the pre-stored interpretation standard. After the first control chip 81 receives the fluorescence analysis value returned by the optical detection device 4 for the fourth time, it determines the positive or negative of the detection item (such as pathogen D) corresponding to the fourth reaction chamber 704 according to the pre-stored interpretation standard. And so on.

[0103] S108, the controller sends the test results to the printer 63 for printing and sends them to the touch screen 62 for display

[0104] The first control chip 81 sends the test results (negative / positive) of each test item and the information read by the scan code to the printer 63. The second control chip 82 controls the printer 63 to print the test report. At the same time, the first control chip 81 sends the test results of each test item to the touch screen 62, and the touch screen 62 switches to another display state to display the test results and the information read by the scan code.

[0105] The aforementioned LAMP detector and detection system, with its internal components utilizing the aforementioned layout and configuration, has a compact structure and small size (the LAMP detector can be as small as 250mm×120mm×80mm). Powered by a battery 61, it integrates nucleic acid amplification and detection, automatic code scanning, and display and printing of test results, making it portable and easy to use. The user simply inserts the loaded LAMP chip 7 to automatically complete code scanning, constant temperature heating amplification, fluorescence detection analysis, and display and printing of test results, thereby improving detection efficiency. The employed LAMP chip 7 can prevent the reagents in each reaction tank from interfering with each other without the need for a control valve or piston, thus avoiding contamination. Samples can be added to only one sample port 701, making it convenient to use. Due to the lack of a control valve or piston, the LAMP chip 7 is relatively small overall (can be as small as 34mm×58mm×3mm), significantly reducing the overall size of the corresponding LAMP detector and making it suitable for handheld detection.

[0106] As used in this specification and claims, the terms "comprises" and "include" merely indicate the inclusion of the steps and elements specifically identified, and these steps and elements do not constitute an exclusive list; a method or apparatus may also include other steps or elements. The term "and / or" as used herein includes any combination of one or more of the associated listed items.

[0107] It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Furthermore, terms such as "upper," "lower," "left," and "right" used in this disclosure are intended solely to describe the relative positions of the components of the disclosure as shown in the accompanying drawings.

[0108] It will be further understood that the terms "first," "second," and the like are used to describe various types of information, but such information should not be limited to these terms. These terms are used solely to distinguish information of the same type from one another and do not indicate a particular order or level of importance. In fact, the terms "first," "second," and the like are fully interchangeable. For example, first information could be referred to as second information, and similarly, second information could be referred to as first information without departing from the scope of this disclosure.

[0109] The above embodiment is intended only to illustrate the technical concepts and features of the present invention and is a preferred embodiment. Its purpose is to enable those skilled in the art to understand the contents of the present invention and implement them accordingly. It is not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications made based on the principles of the present invention are intended to be covered by the scope of protection of the present invention.

Claims

1. A handheld LAMP detector, characterized in that: include: A housing having an inner cavity and a chip port for inserting a LAMP chip; a chip seat disposed in the inner cavity, the chip seat having an accommodation space for accommodating the LAMP chip, the accommodation space being opposite to the chip port; A heating component, which is used to heat the LAMP chip at a constant temperature, and the heating component is arranged in the inner cavity; an optical detection device, which is movably disposed in the inner cavity in a left-right direction, and has a plurality of detection positions, each of which is used to correspond to a plurality of reaction chambers on the LAMP chip; a driving mechanism, configured to drive the optical detection device to move in a left-right direction, the driving mechanism being disposed in the inner cavity; a battery, used to power the heating assembly, the optical detection device, and the driving mechanism, the battery being disposed in the inner cavity; A touch screen, used for displaying the detection result, wherein the touch screen is provided on the upper side wall of the housing and is located above the battery; A printer, used for printing the test results, wherein the printer is embedded in the upper side wall of the housing and located above the battery; a code reader for reading barcode information, wherein the barcode information includes sample information and / or user information, and the code reader is embedded in the front side wall of the housing; The chip holder includes a fixing portion and a supporting portion located at the rear side of the fixing portion. The accommodating space includes a slot provided on the fixing portion for inserting the LAMP chip. The slot passes through the fixing portion in a front-to-back direction. The slot is configured such that after the LAMP chip is inserted, the rear portion of the LAMP chip extends out of the slot to face the optical detection device. The supporting portion has a supporting surface facing the rear portion of the LAMP chip. The heating assembly is arranged on the supporting surface of the chip seat, and the heating assembly includes a heat conducting plate located on the supporting surface and a pressing spring for pressing the LAMP chip onto the heat conducting plate. The pressing spring is fixedly arranged on the fixing seat and is elastically deformable. The pressing spring is configured to bend and deform after the LAMP chip is inserted so as to press the LAMP chip onto the heat conducting plate. The LAMP chip is configured to have a plurality of reaction chambers arranged in parallel along a left-right direction, and the plurality of reaction chambers are arranged on a rear portion of the LAMP chip extending out of the slot.

2. The handheld LAMP detector according to claim 1, characterized in that: The chip port is arranged on the front side wall of the shell, the chip seat is located at the front of the inner cavity, the battery is located at the rear side of the inner cavity, the heating component is arranged on the chip seat, at least part of the optical detection device is located above the chip seat, and the driving mechanism is arranged between the chip seat and the battery.

3. The handheld LAMP detector according to claim 1, characterized in that: The heating component also includes a heating film, thermal insulation cotton, a temperature sensor and a fuse. The thermal insulation cotton is arranged on the chip seat, the heating film is arranged on the thermal insulation cotton, the heat conducting plate is arranged on the heating film, and the temperature sensor and the fuse are embedded in the thermal insulation cotton.

4. The handheld LAMP detector according to claim 1, characterized in that: The driving mechanism includes a guide rail extending in the left-right direction and arranged on the inner wall of the shell, a mounting block movably arranged on the guide rail in the left-right direction, and a motor for driving the mounting block to move in the left-right direction, the motor is arranged on the inner wall of the shell, and the optical detection device is arranged on the mounting block.

5. The handheld LAMP detector according to claim 1, characterized in that: The battery is a rechargeable battery, and a power interface electrically connected to the battery is provided on the rear side of the shell.

6. The handheld LAMP detector according to claim 1, characterized in that: The touch screen has a plurality of display states. In one of the display states, the touch screen has an instruction input area for a user to input instructions.

7. The handheld LAMP detector according to claim 1, characterized in that: The lower part of the shell is provided with a recessed part for easy gripping by fingers.

8. A handheld LAMP detection system, comprising a LAMP detector and a LAMP chip, characterized in that: The LAMP detector is a handheld LAMP detector according to any one of claims 1 to 7.

9. The handheld LAMP detection system according to claim 8, characterized in that: The LAMP chip includes a chip body, which is provided with a sample addition port, multiple reaction chambers and multiple exhaust ports. Each reaction chamber is connected to the sample addition port through a first microchannel, and each first microchannel has one or more bending portions. Each reaction chamber is also connected to a corresponding exhaust port through a second microchannel. The bending portion includes an arc-shaped arc-shaped portion as a whole, and the central angle of the arc of the arc-shaped portion is greater than 90 degrees. The arc-shaped bending portion includes a first arc-shaped bending portion and a second arc-shaped bending portion. The center of the first arc-shaped bending portion is located on its right side, and the center of the second arc-shaped bending portion is located on its left side.

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