Contamination-resistant sealed cap with barrier filter, multi-chamber sample preparation device

Abstract

The present invention provides an anti-pollution sealing cover and a multi-chamber sample preparation device provided with a barrier filter, wherein the anti-pollution sealing cover comprises a cover body and a base that are buckled in opposite directions, a sealed cavity is formed between the cover body and the base, and an air intake gap is formed between the cover body and the base, a barrier filter is installed in the air intake gap, the barrier filter can intercept the nucleic acid aerosol in the gas entering the air intake gap and allow the gas to pass through, an air hole connected to the air intake gap is constructed on the base, and the side of the base away from the cover body can be sealed and connected to the multi-chamber sample preparation box. The present invention can intercept the nucleic acid aerosol that may exist in the inlet and outlet airflows, thereby preventing the aerosol from entering the system and causing aerosol pollution.

Description

Technical Field

[0001] The invention belongs to the technical field of nucleic acid molecule diagnosis, and in particular relates to an anti-pollution sealing cover provided with a barrier filter element and a multi-chamber sample preparation device. Background Art

[0002] With the continuous development of in vitro diagnostic technology and the continuous exploration of precision medicine, molecular diagnostic technology has gradually been accepted by the majority of medical testing practitioners due to its advantages such as high sensitivity and fast detection speed. Among them, PCR products occupy the main market of molecular diagnosis. PCR products have high sensitivity, strong specificity, short diagnostic window period, and can be qualitatively or quantitatively detected. They are widely used in the diagnosis of infectious diseases, eugenics, genetic disease genes, tumors and other diseases. From sample collection to obtaining test results, PCR testing requires sample pretreatment, nucleic acid extraction and purification, PCR amplification, software analysis and interpretation, etc., which brings a series of problems, such as the need for multiple professional equipment, complex operation steps, high requirements for practitioners' operating skills, long time consumption, laboratory contamination, etc.

[0003] In order to solve the above problems, many fully integrated microfluidic chip products have appeared in recent years. An instrument is equipped with a multifunctional integrated chip. The chip integrates all the reagents required for the reaction, which can realize the whole process of sample in-result out. Among them, there is a type of microfluidic chip product that controls the flow of liquid in the chip through the suction of a micropump valve. During the suction process of the pump valve, the air in the environment will be brought into the chip with the suction. If the test environment is contaminated by nucleic acid aerosols, the subsequent detection chip will cause false positive problems due to aerosol contamination. Therefore, when designing this type of integrated chip, in addition to considering the storage of multiple reagents and the design of flow path functions, it is also necessary to focus on the anti-pollution design of the gas exchange part inside and outside the chip. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to provide an anti-pollution sealing cover and a multi-chamber sample preparation device equipped with a barrier filter element, which can intercept nucleic acid aerosols that may exist in the inlet and outlet air flows, thereby preventing the aerosols from entering the system and causing aerosol contamination problems.

[0005] In order to solve the above problems, the present invention provides an anti-pollution sealing cover provided with a barrier filter element, comprising a cover body and a base that are buckled together, a sealed cavity is formed between the cover body and the base, and an air intake gap is formed between the cover body and the base, a barrier filter element is installed in the air intake gap, the barrier filter element can intercept nucleic acid aerosols in the gas entering the air intake gap and allow the gas to pass through, an air hole connected to the air intake gap is constructed on the base, and the side of the base away from the cover body can be sealed and connected to the multi-chamber sample preparation box.

[0006] In some embodiments, the barrier filter element is electrically charged, preferably positively charged; and / or the barrier filter element is made of a hydrophobic material.

[0007] In some embodiments, the barrier filter element is a porous structure made of powdered polymer material by high temperature sintering or stamping, and the pore size of the porous structure is between 0.05 μm and 10 μm.

[0008] In some embodiments, a first through hole is penetrated at the center position of the base, and a second through hole corresponding to the first through hole is provided at the center position of the cover body, the air intake gap is formed between the opening of the second through hole and the opening of the first through hole, and the upper and lower end faces of the barrier filter element are clamped by the opening of the second through hole and the opening of the first through hole.

[0009] In some embodiments, the base has a sealing platform extending toward one side of the cover body at the opening position of the first through hole, and the plurality of air holes are constructed on the sealing platform.

[0010] In some embodiments, the sealing platform is an annular boss, the inner diameter of the annular boss is larger than the apertures of the first through hole and the second through hole, and the barrier filter element is cylindrical.

[0011] In some embodiments, a sealing membrane is connected to the sealing platform.

[0012] In some embodiments, the cover body is screw-connected to the base; and / or a sealing ring is provided between the cover body and the base so that gas can only enter the sealed cavity through the barrier filter element.

[0013] In some embodiments, a side of the base facing away from the cover body has a sealing rib, and the sealing rib separates the plurality of air holes one by one into independent spaces.

[0014] The present invention further provides a multi-chamber sample preparation device, comprising a multi-chamber sample preparation box body, wherein the top opening of the multi-chamber sample preparation box body is sealedly connected to the above-mentioned anti-pollution sealing cover provided with a barrier filter element.

[0015] The present invention provides an anti-pollution sealing cover and a multi-chamber sample preparation device provided with a barrier filter. When the corresponding multi-chamber sample preparation box needs to have gas in and out, the barrier filter can intercept nucleic acid aerosols that may exist in the inlet and outlet airflows, thereby preventing the aerosols from entering the system and causing aerosol contamination problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1It is a schematic diagram of the three-dimensional structure of the anti-pollution sealing cover according to an embodiment of the present invention;

[0017] Figure 2 for Figure 1 A cross-sectional view of (the curved arrows in the figure show the approximate direction of the airflow);

[0018] Figure 3 for Figure 1 A schematic diagram of the three-dimensional structure of the cover body;

[0019] Figure 4 for Figure 1 Schematic diagram of the three-dimensional structure of the base.

[0020] The reference numerals are:

[0021] 1. Cover body; 11. Cover body annular wall; 12. First radial protrusion; 13. Second through hole; 2. Base; 21. Air hole; 22. First through hole; 23. Base annular wall; 24. Second radial protrusion; 25. Sealing platform; 3. Barrier filter element. DETAILED DESCRIPTION

[0022] See also Figures 1 to 4 As shown, according to an embodiment of the present invention, there is provided an anti-pollution sealing cover provided with a barrier filter element, comprising a cover body 1 and a base 2 which are buckled together, a sealed cavity is formed between the cover body 1 and the base 2, and an air intake gap is formed between the cover body 1 and the base 2, a barrier filter element 3 is installed in the air intake gap, the barrier filter element 3 can intercept the nucleic acid aerosol in the gas entering the air intake gap and allow the gas to pass through, an air hole 21 connected to the air intake gap is constructed on the base 2, and a side of the base 2 away from the cover body 1 can be sealed and connected to a multi-chamber sample preparation box, it can be understood that the barrier filter element 3 has a breathable microporous material to ensure that air can pass through in both forward and reverse directions, specifically, the barrier filter element 3 is a porous structure made of powdered polymer material by high-temperature sintering or stamping, and the pore size of the porous structure is between 0.05μm-10μm. In this technical solution, when the corresponding multi-chamber sample preparation box needs to have gas in and out, the barrier filter 3 can physically intercept the nucleic acid aerosol that may exist in the inlet and outlet airflow, thereby preventing the aerosol from entering the system and causing aerosol contamination problems.

[0023] Furthermore, the barrier filter 3 is positively charged, so that the barrier filter 3 can be combined with an electrostatic adsorption function on the basis of physically intercepting the nucleic acid aerosol, thereby further improving the interception effect of the aerosol; and / or, the barrier filter 3 is made of hydrophobic material, so that the barrier filter 3 can adsorb water vapor in the airflow to affect the air permeability. It can be understood that the smaller the pore size of the barrier filter 3, the higher the interception efficiency of the nucleic acid aerosol, but the smaller the pore size, the lower the air permeability, so the actual air volume used by the sealing cover should be taken into account for screening. And the best, the barrier filter 3 can only pass gas, but not liquid and solid. In some embodiments, the barrier filter 3 is one of nylon, Teflon, polypropylene, and polyethylene, preferably a filter made of polyethylene sintering process, which can effectively block nucleic acid aerosols.

[0024] In some embodiments, a first through hole 22 is penetrated at the center position of the base 2, and a second through hole 13 corresponding to the first through hole 22 is formed at the center position of the cover body 1, and the air intake gap is formed between the orifice of the second through hole 13 and the orifice of the first through hole 22, and the upper and lower end surfaces of the barrier filter element 3 are clamped by the orifice of the second through hole 13 and the orifice of the first through hole 22, and the first through hole 22 and the second through hole 13 are connected in the axial direction of the sealing cover to form a passage for the suction pump valve of the multi-chamber sample preparation box body, thereby meeting the assembly requirements of relevant instruments and equipment, and at the same time, the orifices of the two also form the air intake gap and clamp the barrier filter element 3, so that the structure of the sealing cover is simple and compact.

[0025] In some embodiments, the base 2 has a sealing platform 25 extending toward one side of the cover body 1 at the opening of the first through hole 22, and the plurality of air holes 21 are constructed on the sealing platform 25. Furthermore, the sealing platform 25 is an annular boss, and the inner diameter of the annular boss is larger than the apertures of the first through hole 22 and the second through hole 13. The barrier filter element 3 is cylindrical, so that the inner hole of the annular boss forms a positioning structure of the barrier filter element 3, so that the position of the barrier filter element 3 is more stable, and the central through hole of the cylindrical barrier filter element 3 can avoid the suction rod of the corresponding instrument.

[0026] The sealing platform 25 is connected with a sealing film (not shown in the figure). When the preparation box body is sealed with the sealing cover and the reagent is loaded in the preparation box body, the sealing film can be fixed on the sealing platform 25 by means of heat pressing, ultrasonic welding, etc. to prevent the liquid reagent from spilling or volatilizing and prevent the solid reagent from getting damp and deteriorating. When the user (i.e., the operator) uses it, the sealing film is uncovered, and the air hole 21 is restored to a smooth state, thereby realizing the function of sucking and controlling the liquid through the pump valve.

[0027] In some embodiments, the cover body 1 and the base 2 are screwed together, and the screwed connection can be a rotational engagement or a threaded screwing. Specifically, the base 2 has a base ring wall 23 extending toward one side of the cover body 1. When the cover body 1 is buckled with the base 2, the cover body ring wall 11 of the cover body 1 and the base ring wall 23 are sealed together, wherein the inner wall of the cover body ring wall 11 has a plurality of first radial protrusions 12 arranged at intervals in the circumferential direction, and the outer wall of the base ring wall 23 has a plurality of second radial protrusions 24 arranged at intervals in the circumferential direction. The plurality of first radial protrusions 12 and the plurality of second radial protrusions 24 are staggered with each other and can form axial overlap under the screwing action of the operator, thereby realizing the axial connection between the cover body 1 and the base 2. At this time, a sealing ring (not shown in the figure) is provided on the top end surface of the base ring wall 23 to further seal the axial mating surface between the cover body 1 and the base 2, so that the gas can only enter the sealing cavity through the barrier filter element 3. When the screw-on connection is a threaded screw-on connection, the sealing ring may not be provided, and sealing can be achieved by interference fit between the threads during the tightening process.

[0028] In some embodiments, the base 2 has a sealing rib (not shown) on the side facing away from the cover body 1, and the sealing rib separates the multiple air holes 21 one by one into independent spaces. The independent spaces correspond to the multiple chambers of the multi-chamber sample preparation box and are sealed and connected through the sealing rib.

[0029] According to an embodiment of the present invention, a multi-chamber sample preparation device is also provided, comprising a multi-chamber sample preparation box body, wherein the top opening of the multi-chamber sample preparation box body is sealed and connected with the above-mentioned anti-pollution sealing cover provided with a barrier filter. That is, the base 2 needs to be used in a sealed manner with the multi-chamber sample preparation box body, and the sample preparation box body is a central through hole (arranged concentrically with the first through hole 22) having a plurality of mutually isolated accommodating chambers constructed around it, wherein the accommodating chambers are pre-filled with liquid or solid biological detection reagents, and the base 2 and the preparation box body can be sealed by ultrasonic welding, laser welding, hot melt sealing, etc. In addition to being sealed around the central through hole and the periphery of the preparation box, the base 2 also needs to realize that the plurality of mutually isolated accommodating chambers in the preparation box are independently sealed from each other, so a sealing groove (that is, the aforementioned sealing rib) corresponding to the accommodating chamber wall is provided on the back of the base to facilitate sealing. At least one air hole 21 is provided inside each mutually isolated sealing groove, and the size of the air holes 21 can be equal or unequal, and can be set according to the use requirements, and these air holes 21 can be used for ventilation, liquid injection, etc.

[0030] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0031] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A pollution-proof sealing cover provided with a barrier filter element, characterized in that: The invention comprises a cover body (1) and a base (2) which are buckled together, a sealed cavity is formed between the cover body (1) and the base (2), and an air intake gap is formed between the cover body (1) and the base (2), a barrier filter element (3) is installed in the air intake gap, and the barrier filter element (3) can intercept nucleic acid aerosol in the gas entering the air intake gap and allow the gas to pass through, an air hole (21) connected to the air intake gap is constructed on the base (2), and a side of the base (2) away from the cover body (1) can be sealed and connected to a multi-chamber sample preparation box; a first through hole (22) is penetrated at the center of the base (2), and a first through hole (22) is penetrated at the center of the cover body (1). The cover body (1) is provided with a second through hole (13) corresponding to the first through hole (22), the air intake gap is formed between the opening of the second through hole (13) and the opening of the first through hole (22), and the upper and lower end surfaces of the barrier filter element (3) are clamped by the opening of the second through hole (13) and the opening of the first through hole (22); the base (2) is provided with a sealing platform (25) extending toward one side of the cover body (1) at the opening position of the first through hole (22), and a plurality of the air holes (21) are constructed on the sealing platform (25); the barrier filter element (3) is cylindrical; and the barrier filter element (3) is electrically conductive.

2. The anti-pollution sealing cover with a barrier filter according to claim 1, characterized in that: The barrier filter element (3) is made of hydrophobic material.

3. The anti-pollution sealing cover with a barrier filter according to claim 2, characterized in that: The barrier filter element (3) is positively charged.

4. The anti-pollution sealing cover with a barrier filter according to claim 1, characterized in that: The barrier filter element (3) is a porous structure made of powdered polymer material by high-temperature sintering or stamping, and the pore size of the porous structure is between 0.05 μm and 10 μm.

5. The anti-pollution sealing cover with a barrier filter according to claim 1, characterized in that: The sealing platform (25) is a circular boss, and the inner diameter of the circular boss is larger than the apertures of the first through hole (22) and the second through hole (13).

6. The anti-pollution sealing cover with a barrier filter according to claim 5, characterized in that: A sealing film is connected to the sealing platform (25).

7. The anti-pollution sealing cover with a barrier filter according to claim 1, characterized in that: The cover body (1) and the base (2) are screwed together; and / or a sealing ring is provided between the cover body (1) and the base (2), so that gas can only enter the sealed cavity through the barrier filter element (3).

8. The anti-pollution sealing cover with a barrier filter according to claim 1, characterized in that: A sealing rib is provided on a side of the base (2) facing away from the cover body (1), and the sealing rib separates the plurality of air holes (21) one by one into mutually independent spaces.

9. A multi-chamber sample preparation device, comprising a multi-chamber sample preparation box, characterized in that: The top opening of the multi-chamber sample preparation box is sealed with an anti-pollution sealing cover provided with a barrier filter according to any one of claims 1 to 8.

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