Additive for piglet milk replacer and piglet milk replacer

Abstract

The present invention provides a lactating piglet milk replacer additive and a milk replacer and a preparation method thereof, which are added with 4 strains of lactic acid bacteria. The method of the present invention adopts a raw material pretreatment process and an ultrafine grinding process, improves the digestibility and utilization rate of conventional raw materials such as rice flour, increases soybean concentrated protein, reduces the amount of high-cost milk-derived raw materials, and reduces product costs; at the same time, a composite lactic acid bacteria additive is added to maintain the intestinal health of lactating piglets and effectively reduce the use of antibiotics. Experiments show that the lactating piglet milk replacer product of the present invention has the characteristics of low product cost, good palatability, low diarrhea rate, fast growth rate, strong disease resistance, and no antibiotics.

Description

Technical Field

[0001] The invention relates to an additive for a lactating piglet milk replacer, in particular to an additive suitable for the lactating piglet milk replacer, the corresponding lactating piglet milk replacer and a preparation method thereof. Background Art

[0002] In modern intensive pig production, in order to improve the utilization efficiency of sows and effectively control the vertical transmission of diseases, the breeding method of early weaning of piglets at 3-4 weeks of age is generally adopted (Duncan et al., 1997), or even ultra-early segregated weaning (SEW) at 10-14 days of age. Because young weaned piglets have not yet adapted to solid feed and have low feed intake (Metz, 1990); their digestive system is immature and endogenous enzymes are not suitable for digesting diets based on plant materials; immunogenic components in feed (such as soy protein) are prone to cause intestinal allergic reactions and damage (Dureau et al., 1994; Wang T et al., 2014); their immune system is imperfect and their resistance to pathogens is weak (Vega et al., 2001); coupled with the psychological stress of leaving the sow and mixing with the group and environmental stress such as group transfer (Kelley et al., 1980; Prerremans et al., 2001), weaned piglets often have a series of problems such as growth inhibition and high mortality rate, which has become one of the major challenges faced in pig production.

[0003] In the design of lactating pig milk replacers, it is necessary to solve the digestibility and piglet health issues at the same time to meet the actual production needs. The lactating pig milk replacers currently used usually select whole milk powder, concentrated whey protein powder, lactose and other dairy raw materials with good palatability and easy digestion, and add a large amount of broad-spectrum antibiotics during use to control piglet diarrhea. However, my country's dairy raw materials are very scarce and mainly rely on imports. The price is also very expensive, which increases the cost of breeding. The extensive use of antibiotics also brings a series of negative effects: (1) The problem of drug resistance of pathogens has become prominent. The diseases in pig farms are becoming more and more complicated, and the effect of treatment drugs is getting worse and worse. In order to control the disease, the dosage of drugs has to be increased, which further aggravates the drug resistance problem and threatens human health. (2) The long-term use of large doses of antibiotics destroys the microecological balance of the piglet's intestine, inhibits the growth rate of piglets, and even causes stiff pigs, causing huge losses to pig farms. (3) It causes serious soil and water environmental pollution, threatening human health. (4) The irrational use of antibiotics and other additives leads to drug residues and quality decline in pork products, which not only affects food safety, but also hinders meat products from entering the international market and reduces economic benefits. In recent years, alternative antibiotic products have become a research hotspot in the field of feed additives. Among them, acidifiers, plant extracts (essential oils), enzyme preparations, probiotics, oligosaccharides, antimicrobial peptides, etc. have been studied in depth on their effects and mechanisms (Su Dingding et al., 2011; Hu Ruizhi et al., 2018; Cui Haiyan et al., 2018; Liu Jun et al., 2018). Among the many products that replace antibiotics, research and application practice have proved that lactic acid bacteria have significant effects in the suckling piglet stage: lactic acid bacteria tolerate the acidic environment of the gastrointestinal tract, inhibit the growth of harmful bacteria and restore the imbalanced microecological balance; they can competitively inhibit the colonization of pathogens in the intestine, inhibit the attachment of pathogens to intestinal cells, and form a good barrier effect; lactic acid bacteria can stimulate the animal intestine, promote the development and maturation of immune organs, produce local immune responses, increase the body's antibody levels or the activity of macrophages, and enhance the body's immune function (increase vaccine potency); lactic acid bacteria can produce volatile fatty acids such as lactic acid, acetic acid, propionic acid and butyric acid in the intestine, produce proteases, lipases, amylases, synthesize nutrients such as vitamins and amino acids, increase the growth rate of animals, and improve breeding efficiency. Summary of the invention

[0004] In view of the above-mentioned existing problems and development trends in the prior art, the present invention first provides a lactic acid bacteria composition specifically suitable for piglet milk replacer, which is composed of the following four strains: Lactobacillus acidophilus, with a strain preservation number of CGMCC No.7.423; Bifidobacterium longum subsp.longum, with a strain preservation number of CGMCC No.7.427; Lactobacillus plantarum, with a strain preservation number of CGMCC No.7.426; and Enterococcus faecium, with a strain preservation number of CGMCC No.7.428. The above four strains were deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on July 1, 2021, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Among them, in the lactic acid bacteria composition, the added mass ratio of each strain is 1:1:1:1.

[0005] Specifically, the specific method for obtaining the Lactobacillus acidophilus and the relevant application parameters are:

[0006] Strain number: SY-R1; Collection location: Beijing; Specific collection location: Xiajiashan pig farm, Beijing pig breeding center; Separation substrate: piglet stomach; Separation method: plate streak method; Culture medium (ingredients g / L): peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, Tween 801.0g, diammonium hydrogen citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.1g, manganese sulfate 0.05g, potassium dihydrogen phosphate 2.0g; Characteristics: Cultured on anaerobic agar plates, forming small (about 0.5mm in diameter), net-shaped, raised, rough surface, and curled edge colonies; Cell shape: bacillus; Culture temperature: 37℃; Culture time: 16-18h; Whether to amplify: yes; Colony count requirement before mixing: ≥10 6 ; After 16srRNA amplification and sequencing analysis, the sequence is shown in SEQ ID NO: 1. Main functions: regulate immunity and balance intestinal flora; secrete antibacterial substances to relieve gastrointestinal inflammation; synthesize vitamins (VB, VK); compete for nutrients and intestinal mucosal positions, and inhibit intestinal colonization of pathogenic bacteria.

[0007] The specific method for obtaining the Bifidobacterium longum subspecies longum and the relevant application parameters are:

[0008] Strain number: SY-R5; Collection location: Beijing; Specific collection location: Xijiashan pig farm, Beijing pig breeding center; Separation substrate: piglet small intestine; Separation method: plate streak method; Culture medium (ingredients g / L): peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, Tween 801.0g, diammonium citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.1g, manganese sulfate 0.05g, potassium dihydrogen phosphate 2.0g, L-cysteine ​​0.5g; Characteristics: Gram-positive, non-motile, rod-shaped cells, one end is sometimes forked, strictly anaerobic bacteria; Cell shape: bacillus; Culture temperature: 37℃; Culture time: 16-18h (anaerobic culture); Whether to amplify: yes; Colony count requirement before mixing: ≥10 6 ; The sequencing sequence is shown in SEQ ID NO: 3. Main functions: regulating immunity, balancing intestinal flora; maintaining intestinal acidic environment, preventing constipation and diarrhea; synthesizing active ingredients such as vitamins and amino acids.

[0009] The specific method for obtaining the plant lactobacillus and the relevant application parameters are:

[0010] Strain number: SY-R4; Collection location: Beijing; Specific collection location: Qutou cattle farm, Yongledian, Tongzhou District, Beijing; Separation substrate: silage corn; Separation method: plate streak method; Culture medium (ingredients g / L): peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, Tween 801.0g, diammonium hydrogen citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.1g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2.0g; Characteristics: anaerobic or facultative anaerobic, the strain is straight or curved rod-shaped, single, sometimes paired or chain-shaped, the optimal pH is about 6.5, and it belongs to homofermentative lactic acid bacteria; Cell shape: bacillus; Culture temperature: 37℃; Culture time: 16-18h; Whether to amplify: yes; Colony count requirement before mixing: ≥10 6 ; The sequencing sequence is shown in SEQ ID NO: 2. Main functions: strong stress resistance, acid and alkali resistance, and can ferment a variety of carbon sources; secrete organic acids, digestive enzymes and antibacterial components, balance intestinal flora, and improve feed digestibility.

[0011] The specific method for obtaining the Enterococcus faecium and the relevant application parameters are:

[0012] Strain number: SY-R6; Collection location: Beijing; Specific collection location: Beijing Pig Breeding Center Pig Resource Farm; Separation substrate: pig intestine; Separation method: plate streak method; Culture medium (ingredients g / L): peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, Tween 801.0g, diammonium hydrogen citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.1g, manganese sulfate 0.05g, potassium dihydrogen phosphate 2.0g; Characteristics: round or oval, single, paired or short chain-arranged Gram-positive cocci, no spores, no flagella, aerobic or facultative anaerobic bacteria, Enterococcus faecalis observed under oil microscope mostly solitary, less opposite and chain-shaped; Cell shape: coccus; Culture temperature: 37℃; Culture time: 16-18h; Whether to amplify: yes; Colony count requirement before mixing: ≥10 6 The sequence is shown in SEQ ID NO: 4. Main functions: Enhance animal immunity, synthesize antibacterial substances, secrete organic acids, maintain the balance of intestinal flora, and prevent diarrhea.

[0013] In addition to the above physiological characteristics, the lactic acid bacteria composition of the present application has the following characteristics: high temperature resistance, adaptability to the strongly acidic gastric juice environment of piglets, and choline resistance:

[0014] Han Qinggong et al. (2018) showed that the survival rate of Lactobacillus plantarum can reach 88.9% when treated at 70°C for 2 minutes, but when the temperature is raised to 80°C for 2 minutes, the survival rate drops to 34.95%. The composite bacteria of the lactic acid bacteria composition of the present invention have a survival rate of more than 96% when treated at 65°C for 2.5 minutes, 5 minutes and 7.5 minutes, and 85°C for 2.5 minutes, which has almost no effect on the survival rate and is suitable for high temperature application conditions such as boiling water brewing;

[0015] The pH in the stomach of piglets is 5-6 when they are born, and drops to about 4 after a few hours. The pH in the stomach remains at about 3 before 2 months of age. The composite lactic acid bacteria of the present invention has a survival rate of 80.54% when treated at pH 3 for 90 minutes, and a survival rate of more than 90% when treated at pH 4, 5, and 6 for 90 minutes.

[0016] The bile salt content in the digestive tract of piglets is 0.03% to 0.3%. The survival rate of the composite lactic acid bacteria of the present invention is 73.52% after being treated with 0.3% pig bile salt for 1 hour.

[0017] At the same time, the composite lactic acid bacteria of the present invention have an inhibitory effect on Escherichia coli, with an inhibition zone diameter of 10.2 mm; it has the strongest tolerance to bacitracin zinc and methylene salicylic acid bacitracin, with a minimum inhibitory concentration (MIC) greater than 1280 mg / kg, followed by flavonoids, followed by oxytetracycline calcium, monensin, salinomycin, and chlortetracycline, all of which have relatively strong tolerance.

[0018] In summary, the additive composed of the composite lactic acid bacteria composition composed of the above four strains can be used as an important component of piglet feed premix (or feed / milk replacer). The premix (or feed / milk replacer) added with the lactic acid bacteria composition does not need to add any form of antibiotics, and can also meet the requirements of low diarrhea rate, fast growth rate, strong disease resistance, and no antibiotics in piglets.

[0019] Furthermore, the present invention also relates to a lactating piglet milk replacer added with the lactic acid bacteria composition and a preparation method thereof.

[0020] For suckling piglets aged 7-28 days, the present invention provides a cost-effective suckling piglet milk replacer for replacing lactation or supplementary feeding during lactation. Specifically, the raw materials and content formula of the suckling piglet milk replacer of the present invention are (each component is calculated by weight, a total of 1000 parts):

[0021]

[0022]

[0023] Among them, the raw material content of the lactating piglet premix (compound premix additive) is (each component is calculated by mass fraction):

[0024]

[0025]

[0026] Among them, the multivitamin (vitamin premix) for suckling piglets includes the following raw materials (each component is measured by mass fraction):

[0027] Product Name Common Name Mass percentage (%) Antioxidants Ethoxyquinoline 0.4～0.6 VB6 (98%) Pyridoxine Hydrochloride 1.3～1.7 Vitamin A Vitamin A 5.5～7.2 Vitamin E (a-tocopherol) dl-α-Tocopheryl acetate 18～22 VB1 (98%) Thiamine nitrate 0.9～1.1 VB12 (1%) Cyanocobalamin 1.1～1.3 Calcium pantothenate (99%) D-Calcium Pantothenate 9～11 Biotin (2%) D-Biotin 3.6～4.4 VK3 (94%) Menadione sodium bisulfite 1.8～2.2 Vitamin D3 Vitamin D3 1.8～2.2 VB2 (80%) Riboflavin 2.7～3.3 Folic acid (99%) Folic acid 0.4～0.6 Niacinamide 98% Niacinamide 10.8～13.2 Rice Husk Powder Rice Husk Powder 42.7～29.2

[0028] Among them, the raw material content of the multi-mineral (trace element premix) for suckling piglets is (each component is calculated by mass):

[0029] Product Name Common Name Mass percentage (%) 1% Calcium Iodate Calcium iodate 45～50 1% Sodium Selenite Sodium selenite 27～32 1% Cobalt Chloride Cobalt chloride 13.5～16.5 Zeolite powder Zeolite powder 14.5～1.5

[0030] Among them, the preparation method of organic multi-mineral (trace element organic premix) for lactating piglets (each component is calculated by mass fraction):

[0031] Product Name Common Name Mass percentage (%) Protein Copper PCU-10 Copper Proteinate 9～10 Protein Iron PFE-15 Protein Iron 31.5～34 Protein Zinc PZN-15 Zinc Proteinate 18～22 Celexan Yeast Selenium 18～22 0.2% Chromium Picolinate Premix Thinner 9～11 Zeolite powder Zeolite powder 14.5～1

[0032] The raw material content of 0.2% chromium picolinate premix is ​​(each component is calculated by mass fraction):

[0033] Product Name Common Name Mass percentage (%) 1.2% Chromium Picolinate Chromium Picolinate 16.6 Zeolite powder Zeolite powder 33.4 Rice bran meal Rice bran meal 50

[0034] Furthermore, the present invention also provides a method for preparing a piglet milk replacer.

[0035] Specifically, the method for preparing the piglet milk replacer of the present invention comprises the following steps:

[0036] 1. Prepare 0.2% chromium picolinate premix according to the raw material formula;

[0037] 2. Prepare the lactic acid bacteria composition, multi-mineral for suckling piglets, organic multi-mineral for suckling piglets and multi-vitamin for suckling piglets according to the respective raw material formula ratios;

[0038] 3. Prepare premix for suckling piglets according to the raw material formula;

[0039] 4. Evenly mix the composite premix with other components according to the raw material formula to obtain a finished product of suckling piglet milk replacer.

[0040] To ensure the effect of the product, high-quality rice (or broken rice) and fish meal (rice puffed by wet method) must be ultra-finely ground, and the particle size must reach 95% over 60 mesh.

[0041] The above raw materials of the present invention are all commercially available raw materials.

[0042] The multi-stage premixing of the production process ensures the uniformity of mixing. All raw materials involved in the formula of piglet milk replacer and compound premix can only be accepted for storage after passing the quality inspection of the quality control department. Each component is accurately measured using an electronic scale according to the formula ratio, and after being put into the mixer and mixed evenly, it can be weighed, packaged and stored. The entire production process is strictly operated in accordance with the requirements of the ISO9001 quality management system and the HACCP food safety management system.

[0043] The milk replacer of the present invention involves the addition of 4 composite lactic acid bacteria, adopts a raw material pretreatment process and an ultrafine grinding process, improves the digestion and utilization rate of the raw materials, reduces the amount of high-cost milk source raw materials in the milk replacer product, and reduces the product cost; at the same time, the added 4-strain lactic acid bacteria composition maintains the intestinal health of suckling piglets and effectively reduces the use of antibiotics.

[0044] Specifically, the milk replacer of the present invention is suitable for feeding or supplementary feeding of piglets aged 7 to 28 days. The application method is: it can be fed directly; the feeding effect is better after mixing with four times warm water (1:4 mass ratio). Experiments show that the lactating piglet milk replacer product of the present invention has the characteristics of low product cost, good palatability, low diarrhea rate, fast growth rate, strong disease resistance, and no antibiotics. DETAILED DESCRIPTION

[0045] To facilitate those skilled in the art to better understand the present invention, the piglet milk replacer provided by the present invention is further described below in conjunction with specific embodiments to illustrate the purpose, functional characteristics and beneficial effects of the present invention. Specific embodiments of the present invention are now given as follows.

[0046] Embodiment: Example of preparing formula of lactating piglet milk replacer

[0047] Preparation method of lactating piglet milk replacer (each component is calculated by weight):

[0048]

[0049]

[0050] Preparation method of lactating piglet premix (each component is calculated by weight):

[0051]

[0052]

[0053] Preparation method of multivitamin for suckling piglets (each component is calculated by weight):

[0054] Serial number Product Name Common Name Example 1 Example 2 Example 3 1 Antioxidants Ethoxyquinoline 0.4 0.6 0.5 2 VB6 (98%) Pyridoxine Hydrochloride 1.3 1.7 1.5 3 Vitamin A Vitamin A 5.5 7.2 6.35 4 Vitamin E (a-tocopherol) dl-α-Tocopheryl acetate 18 22 20 5 VB1 (98%) Thiamine nitrate 0.9 1.1 1 6 VB12 (1%) Cyanocobalamin 1.1 1.3 1.2 7 Calcium pantothenate (99%) D-Calcium Pantothenate 9 11 10 8 Biotin (2%) D-Biotin 3.6 4.4 4 9 VK3 (94%) Menadione sodium bisulfite 1.8 2.2 2 10 Vitamin D3 Vitamin D3 1.8 2.2 2 11 VB2 (80%) Riboflavin 2.7 3.3 3 12 Folic acid (99%) Folic acid 0.4 0.6 0.5 13 Niacinamide 98% Niacinamide 10.8 13.2 12 14 Rice Husk Powder Rice Husk Powder 42.7 29.2 35.95 total 100 100 100

[0055] Preparation method of multi-mineral for suckling piglets (each component is calculated by weight):

[0056]

[0057]

[0058] Preparation method of organic multi-mineral for suckling piglets (each component is calculated by weight):

[0059] Serial number Product Name Common Name Example 1 Example 2 Example 3 1 Protein Copper PCU-10 Copper Proteinate 9 10 9.5 2 Protein Iron PFE-15 Protein Iron 31.5 34 32.75 3 Protein Zinc PZN-15 Zinc Proteinate 18 22 20 4 Celexan Yeast Selenium 18 22 20 5 0.2% Chromium Picolinate Premix Thinner 9 11 10 6 Zeolite powder Zeolite powder 14.5 1 7.75 total 100 100 100

[0060] Preparation method of 0.2% chromium picolinate premix (components are calculated by weight):

[0061] Serial number Product Name Common Name Weight(kg) 1 1.2% Chromium Picolinate Chromium Picolinate 16.6 2 Zeolite powder Zeolite powder 33.4 3 Rice bran meal Rice bran meal 50 total 100

[0062] Test example: Effect test of compound milk replacer for piglets

[0063] 1. Materials and Methods

[0064] 1.1 Test materials

[0065] The lactating piglet milk replacer prepared in the above preparation examples (Examples 1-3 above) was the diet for the test group; a lactating piglet milk replacer previously produced by the applicant company, which was fed according to the product instructions, was the control group.

[0066] The formula of the control milk replacer is (by weight): 301 parts of puffed corn; 300 parts of whole milk powder; 120 parts of whey powder; 50 parts of white sugar; 116 parts of concentrated whey protein; 40 parts of chicken liver powder; 25 parts of yeast hydrolyzate; 8 parts of acidity regulator; 5 parts of stone powder; 10 parts of monocalcium phosphate; and 25 parts of compound premixed feed.

[0067] Among them, the effective ingredients of compound premix feed are shown in the following table:

[0068]

[0069] 1.2 Experimental animals and groups

[0070] The experiment selected 160 healthy weaned piglets at 14 days of age and randomly divided them into 4 treatment groups, with 4 replicates in each treatment group and 10 piglets in each replicate, half male and half female, for a 14-day feeding experiment; among them, the experimental pigs began to supplement the corresponding milk replacer at around 7 days of age, and then weaned with the corresponding milk replacer from 15 to 28 days of age. The specific experimental groups are shown in the table below:

[0071] Trial Groups Feed category Control group Existing lactating piglet milk replacer Test Group 1 Example 1 Milk Replacer Test Group 2 Example 2 Milk Replacer Experiment 3 groups Example 3 Milk Replacer

[0072] 1.3 Daily management of the trial

[0073] The feeding and management of piglets were consistent with those on the farm, with free access to food and water. The control group and the experimental group were raised by dedicated personnel.

[0074] 1.4 Measurement items and methods

[0075] (1) Piglet production performance: initial weaning weight, final weight, average daily weight gain, average daily feed intake, and feed-to-weight ratio;

[0076] (2) Diarrhea index of piglets;

[0077] 2. Test results and analysis

[0078] 2.1 Effects of different milk replacers on the production performance of early weaned piglets

[0079] Control group Test Group 1 Test Group 2 Experiment 3 groups Initial weight at 15 days old (kg) 5.34±0.06a 5.33±0.07a 5.31±0.06a 5.31±0.08a Final weight at 28 days old (kg) 7.24±0.19a 7.61±0.10a 7.56±0.09a 7.53±0.14a Average daily feed intake (kg) 226.33±15.06b 267.90±2.32Ba 264.00±2.38a 263.54±10.75a Average daily weight gain (kg) 158.54±11.6b 189.71±3.07Ba 187.71±3.61a 184.79±5.87a Feed to meat ratio 1.43±0.02a 1.41±0.03a 1.41±0.02a 1.43±0.04a

[0080] Note: Data in the same category have the same letter in the shoulder, indicating no significant difference (P>0.05), different lowercase letters in the shoulder, indicating significant difference (P<0.05), and uppercase letters in the shoulder, indicating extremely significant difference (P<0.01).

[0081] 2.2 Effects of different milk replacers on diarrhea performance in piglets

[0082] Some pigs in the control group and each experimental group had diarrhea on the day of weaning, and no drug treatment was given.

[0083] 2.3 Effects of different milk replacers on piglet skin and coat color

[0084] There was no significant difference in skin color and fur color between the control group and the experimental groups.

[0085] 2.4 Analysis of test results

[0086] The test results show that the suckling piglets weaned at 15 days of age used the suckling piglet milk replacer prepared according to Examples 1-3. During the test period, the piglets were in good health and had normal coat color; the average daily feed intake and daily weight gain were good, and were significantly higher than the control group (commercially available prior art products).

[0087] Compared with the above-mentioned comparative example, the product of the present invention mainly replaces puffed corn with puffed rice flour, increases soy protein concentrate and fish meal, reduces the amount of concentrated whey protein and whole milk powder, and reduces the cost per ton by about 1,000 yuan; in addition, the comparative example product contains commonly used feed antibiotic additives: 3000PPM of chlortetracycline and 2000PPM of kitasamycin, while the example milk replacer product of the present invention does not contain any antibiotic additives. The test results show that the diarrhea rate and disease resistance can reach the level of adding antibiotics.

[0088] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention. Sequence Listing <110> Beijing Sanyuan Seed Technology Co., Ltd. Feed Branch <120> Additive for piglet milk replacer and piglet milk replacer <130> P21100002 <160> 4 <170> SIPOSequenceListing 1.0 <210> 1 <211> 1473 <212> DNA <213> Lactobacillus acidophilus <400> 1 atacatgcag tcgagcgagc tgaaccaaca gattcacttc ggtgatgacg ttgggaacgc 60 gagcggcgga tgggtgagta acacgtgggg aacctgcccc atagtctggg ataccacttg 120 gaacaggtg ctaataccgg ataagaaagc agatcgcatg atcagcttat aaaaggcggc 180 gtaagctgtc gctatgggat ggccccgcgg tgcattagct agttggtagg gtaacggcct 240 accaaggcaa tgatgcatag ccgagttgag agactgatcg gccacattgg gactgagaca 300 cggcccaaac tcctacggga ggcagcagta gggaatcttc cacaatggac gaaagtctga 360 tggagcaacg ccgcgtgagt gaagaggtt ttcggatcgt aaagctctgt tgttggtgaa 420 gaaggataga ggtagtaact ggcctttat tgacggtaat caaccagaaa gtcacggcta 480 actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga tttattgggc 540 gtaaagcgag cgcaggcgga agataagtc tgatgtgaaa gccctcggct taaccgaga 600 actgcatcgg aaactgtttt tcttgagtgc agaagagg agtggaactc catgtgtagc 660 ggtggaatgc gtagatatat ggaagaacac cagtggcgaa gcggctctct ggtctgcaac 720 tgacgctgag gctcgaaagc atgggtagcg aacaggatta gataccctgg tagtccatgc 780 cgtaaacgat gagtgctaag tgttgggagg tttccgctc tcagtgctgc agctaacgca 840 ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcta gtgcaatccg tagagatacg gagttccctt cggggacact aagacaggtg 1020 gtgcatggct gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttgtca ttagttgcca gcattaagtt gggcactcta atgagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaagtca tcatgcccct tatgacctgg gctacacacg 1200 tgctacaatg gacagtacaa cgaggagcaa gcctgcgaag gcaagcgaat ctcttaaagc 1260 tgttctcagt tcggactgca gtctgcaact cgactgcacg aagctggaat cgctagtaat 1320 cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380 catgggagtc tgcaatgccc aaagccggtg gcctaacctt cgggaaggag ccgtctaagg 1440 cagggcagat gactggggtg aagtcgtaac aag 1473 <210> 2 <211> 1440 <212> DNA <213> Lactobacillus plantarum <400> 2 atacatgcaa gtcgaacgaa ctctggtatt gattggtgct tgcatcatga tttacatttg 60 agtgagtggc gaactggtga gtaacacgtg ggaaacctgc ccagaagcgg gggataacac 120 ctggaaacag atgctaatac cgcataacaa cttggaccgc atggtccgag cttgaaagat 180 ggcttcggct atcacttttg gatggtcccg cggcgtatta gctagatggt ggggtaacgg 240 ctcaccatgg caatgatacg tagccgacct gagagggtaa tcggccacat tgggactgag 300 acacggccca aactcctacg ggaggcagca gtagggaatc ttccacaatg gacgaaagtc 360 tgatggagca acgccgcgtg agtgaagaag ggtttcggct cgtaaaactc tgtgttaaag 420 aagaacatat ctgagagtaa ctgttcaggt attgacggta tttaaccaga aagccacggc 480 taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg 540 gcgtaaagcg agcgcaggcg gttttttaag tctgatgtga aagccttcgg ctcaaccgaa 600 gaagtgcatc ggaaactggg aaacttgagt gcagaagagg acagtggaac tccatgtgta 660 gcggtgaaat gcgtagatat atggaagaac accagtggcg aagcggctgt ctggtctgta 720 actgacgctg aggctcgaaa gtatgggtag caaacaggat tagataccct ggtagtccat 780 accgtaaacg atgaatgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg 840 cattaagcat tccgcctggg gagtacggcc gcaaggctga aactcaaagg aattgagggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcta cgcgaagaac cttaccaggt 960 cttgacatac tatgcaaatc taagagatta gacgttccct tcggggacat ggatacaggt 1020 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1080 aacccttatt atcagttgcc agcattaagt tgggcactct ggtgagactg ccggtgacaa 1140 accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac 1200 gtgctacaat ggatggtaca acgagttgcg aactcgcgag agtaagctaa tctcttaaag 1260 cattctcag ttcggattgt aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa 1320 tcgcggatca gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca 1380 ccatgagagt ttgtaacacc caaagtcggt ggggtaacct tttaggaacc agccgcctaa 1440 <210> 3 <211> 972 <212> DNA <213> Bifidobacterium longum subsp. longum <400> 3 cgcggtatca ccatcaacat cgcccacatc gagtaccaga ccgagaagcg tcactacgct 60 cacgtcgact gcccgggcca cgccgacttc gtgaagaaca tgattaccgg tgctgcccag 120 atggatgcgc tatcctcgtt gtggccgcca ccgacgcccg atggcccaga ctcgcgagca 180 cgtgctgctc gcccgtcagg ttggcgttcc gaagatcctc gtcgccctga acaagtgcga 240 catggtcgac gatgaagagc tcatcgagct cgtcgaagaa gaggtccgcg acctcctcga 300 cgagaacggc ttcgaccgtg actgcccggt catccacacc tccgcttacg gtgctctgca 360 cgacgacgct ccggaccacg agaagtgggt ccagtccgtt aaggacctca tggacgctgt 420 cgacgactac atcccgaccc cggttcacga cctggacaag ccgttcctga tgccgatcga 480 ggacgtcttc accatctccg gccgtggtac cgttgtcacc ggtcgtgtcg agcgtggcca 540 gctgccgtca acaccccggt cgagatcgtt ggtatccgtc cgacccagca gaccaccgtc 600 acctccatcg agaccttcca caagaccatg gacgcctgcg aggctggcga caacaccggt 660 ctgcttctgc gtggtctcgg ccgtgacgat gtcgagcgtg gccaggttgt ggccaagccg 720 ggctccgtca ccccgcacac caagttcgag ggcgaagtct acgtgctgac caaggacgaa 780 ggcggccgtc actcgccgtt cttctccaac taccgtccgc agttctactt ccgcaccacc 840 gacgtcaccg gcgtcatcga gctgcggaag gcgtcgagat ggttcagcgg gcgaccacgc 900 taccttcacc gttgagctga ttcagcccat cgctatggag gaaggcctga ccttcgctgt 960 gcgtgaaggt gc 972 <210> 4 <211> 1495 <212> DNA <213> Enterococcus faecium <400> 4 atacatgcag tcgtacgctt ctttttccac cggagcttgc tccaccggaa aaagaagagt 60 ggcgaacggg tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa 120 caggtgctaa taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg 180 ggtgtcgctg atggatggac ccgcggtgca ttagctagtt ggtgaggtac ggctcaccaa 240 ggccacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacgccc 300 aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt ctgaccgagc 360 aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa 420 ggatgagagt aactgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc ccctgggggc aaagtgacag gtggtgcatg 1020 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 1080 ttgttagttg ccatcattta gttgggcact ctagcaagac tgccggtgac aaaccggagg 1140 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca 1200 atgggaagta caacgagttg cgaagtcgcg aggctaagct aatctcttaa agcttctctc 1260 agttcggatt gcaggctgca actcgcctgc atgaagccgg aatcgctagt aatcgcggat 1320 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga 1380 gtttgtaaca cccgaagtcg gtgaggtaac cttttggagc cagccgccta aggtgggata 1440 gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct ggatc 1495

Claims

1. A composite premix additive for piglet milk replacer, characterized in that: The additive is composed of the following raw materials by mass fraction: vitamin premix 1.6%; trace element inorganic premix 0.4%; trace element organic premix 4%; copper sulfate 1.26-1.54%; manganese sulfate 0.54-0.66%; ferrous sulfate 1.26-1.54%; L-lysine hydrochloride 18-22%; DL-methionine 8.1-9.9%; L-threonine 5.4-6.6%; hydrolyzed tannic acid 3.6-4.4%; zinc oxide 7.2-8.8%; choline chloride 3.6-4.4%; complex enzyme preparation 3.6-4.4%; lactic acid bacteria composition 3.6-4.4%; valine 2.6-3. 2%; 3.6-4.4% saccharomyces cerevisiae; 1.4-1.8% flavoring agent; 1-1.3% clostridium butyricum; 1-1.3% xylo-oligosaccharide; 1-1.3% maclea-hui powder; 1-1.3% ethoxyquinoline; 0.6-0.9% phytase; 0.45-0.58% L-tryptophan; 0.43-0.53% glucose oxidase; 2-19% wheat flour; and 5.76-6.75% stone powder; the additive is added in the piglet milk replacer in an amount of 2.5wt%; the piglet milk replacer is suitable for feeding or supplementing piglets aged 7 to 28 days; the lactic acid bacteria composition is composed of the following raw materials: the accession number is CGMCC Lactobacillus acidophilus with a preservation number of CGMCC No. 7.423, Bifidobacterium longum subsp. longum with a preservation number of CGMCC No. 7.427, Lactobacillus plantarum with a preservation number of CGMCC No. 7.426, and Enterococcus faecium with a preservation number of CGMCC No. 7.428; the added mass ratio of each strain is 1:1:1:

1.

2. The composite premix additive according to claim 1, characterized in that: The vitamin premix consists of the following raw materials by mass fraction: 0.4-0.6% ethoxyquinoline; 1.3-1.7% pyridoxine hydrochloride; 5.5-7.2% vitamin A; 18-22% dl-α-tocopheryl acetate; 0.9-1.1% thiamine nitrate; 1.1-1.3% cyanocobalamin; 9-11% D-calcium pantothenate; 3.6-4.4% D-biotin; 1.8-2.2% sodium menadione bisulfite; 1.8-2.2% vitamin D3; 2.7-3.3% riboflavin; 0.4-0.6% folic acid; 10.8-13.2% nicotinamide; and 29.2-42.7% rice husk powder.

3. The composite premix additive according to claim 1, characterized in that: The trace element inorganic premix consists of the following raw materials by mass fraction: 45-50% calcium iodate; 27-32% sodium selenite; 13.5-16.5% cobalt chloride; and 1.5-14.5% zeolite powder.

4. The composite premix additive according to any one of claims 1 to 3, characterized in that: The trace element organic premix consists of the following raw materials by mass fraction: 9-10% copper protein; 31.5-34% iron protein; 18-22% zinc protein; 18-22% yeast selenium; 9-11% diluent; and 1-14.5% zeolite powder.

5. The composite premix additive according to claim 4, characterized in that: The diluent is composed of the following raw materials by mass fraction: 16.6% of chromium picolinate; 33.4% of zeolite powder; and 50% of rice bran meal.

6. A piglet milk replacer, characterized in that: The piglet milk replacer comprises the composite premixed additive according to any one of claims 1 to 5; the piglet milk replacer is suitable for feeding or supplementary feeding of piglets from 7 days to 28 days old.

7. The piglet milk replacer according to claim 6, characterized in that The piglet milk replacer is composed of the following raw materials by weight: 478-365 parts of puffed rice; 216-264 parts of whole milk powder; 90-110 parts of whey powder; 45-55 parts of white sugar; 36-44 parts of concentrated whey protein; 36-44 parts of chicken liver powder; 22.5-27.5 parts of yeast hydrolyzate; 18-22 parts of fish meal; 18-22 parts of concentrated soy protein; 5.4-8.6 parts of acidity regulator; 3.4-4.7 parts of stone powder; 6.7-8.2 parts of monocalcium phosphate; and 25 parts of the composite premix additive.

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