Device for analyzing multiple biomarkers simultaneously and method for analyzing multiple biomarkers simultaneously

Abstract

Provided is a device for simultaneously analyzing a plurality of biomarkers. According to an embodiment of the present invention, the device for simultaneously analyzing a plurality of biomarkers comprises: a cartridge mounting portion (210), in which a cartridge (100) is mounted, the cartridge (100) comprising one or more wells and a tip (110); a tip connecting portion (220), which is connected to the tip (110) and can move on a predetermined moving trajectory when the tip (110) is connected; an optical unit (230), which irradiates light to the tip (110) and receives light generated from the tip (110) in response to the light irradiation; and a processing unit (240), which uses the light received by the optical unit (230) to determine whether a detection target substance (T) is present in a sample (S) according to a predetermined method.

Description

Technical Field

[0001] The present disclosure relates to an apparatus for simultaneously analyzing multiple biomarkers and a method for simultaneously analyzing multiple biomarkers. Background Art

[0002] Viruses are pathogens with a size of less than 1 μm, which cannot be observed even with an optical microscope, and an electron microscope is required to see the virus with the naked eye. In addition, drug control methods that directly act on viruses have not yet been developed. In the past, electron microscopy and serological methods were mainly used for virus diagnosis. In the method using an electron microscope, the presence of the virus can be confirmed, but it is impossible to diagnose the species based on its morphological characteristics. Among serological methods, the detection sensitivity of the enzyme-linked immunosorbent assay is about 1 / 1000 times that of the most commonly used diagnostic method or the polymerase chain reaction (PCR) diagnostic method, and the diagnosis often fails due to unexpected nonspecific reactions between antibodies and test samples.

[0003] Therefore, accurate and rapid diagnosis is a prerequisite for viral disease management. At the same time, in the reverse transcription polymerase chain reaction (RT-PCR) method commonly used for accurate diagnosis of plant viruses, the primers used determine the specificity and accuracy of the diagnosis. In the case of commonly used primers, these primers are designed for viral gene cloning purposes and are not species-specific in many cases. Due to the presence of non-specific products produced by plants or other viruses, they are generally not suitable for diagnosis. In addition, the RT-PCR method is performed using various types of primers to diagnose multiple types of viruses from the same sample, which requires a lot of diagnostic costs and labor.

[0004] Meanwhile, since in the diagnostic device according to the related art, the reaction is performed by adding a reaction reagent, a detection reagent, etc. to a sample, the frequency of errors in the test results due to each added reagent is relatively high. In order to make an accurate diagnosis, a process of repeating the experiment multiple times is required. If errors due to the addition of reagents can be prevented, the cost and time required for diagnosis can be reduced. Summary of the invention

[0005] The present disclosure provides an analysis device capable of detecting a plurality of biomarkers by analyzing only a single tip because a binding material capable of specifically binding to different detection target substances is formed in the tip.

[0006] The present disclosure also provides an analysis device in which, since a binding material capable of specifically binding to a detection object is formed in a tip and analysis processing is performed while the tip is moved, analysis errors due to components other than a sample can be significantly reduced.

[0007] The present disclosure also provides an analysis device, which detects light signals generated outside the barrel compared to analysis devices according to the prior art that detect light signals generated within the well of the barrel, thereby significantly reducing the occurrence of light signal interference caused by external factors such as contamination and residues.

[0008] The present disclosure also provides an analysis apparatus in which the inconvenience of using different tips to detect different detection target substances and requiring a plurality of analysis processes can be eliminated.

[0009] The present disclosure also provides a method, wherein the analysis device can be used to perform simultaneous analysis of multiple biomarkers.

[0010] According to one aspect of the present disclosure, a device for simultaneously analyzing a plurality of biomarkers is provided, the device comprising: a cartridge mounting portion (210), a cartridge (100) being mounted in the cartridge mounting portion (210), the cartridge (100) comprising at least one well and a tip (110), wherein the at least one well comprises a sample well (101) and a reaction well (105), a sample (S) containing a detection target substance (T) being injected into the sample well (101), the reaction well (105) containing a first binding material (105a) that specifically binds to the detection target substance (T) and to which a marker (R) is coupled, and the tip (110) being inserted into the at least one well. A tip (110) is provided in the well, and a second binding material (111) that specifically binds to a detection object substance (T) is connected to one end of the tip (110); a tip connection part (220) that is connected to the tip (110) and can move on a predetermined movement trajectory while being connected to the tip (110); an optical unit (230) that irradiates light to the tip (110) and receives light generated from the tip (110) according to the light irradiation; and a processing unit (240) that uses the light received by the optical unit (230) to judge / determine whether the detection object substance (T) is present in the sample (S) according to a predetermined method.

[0011] The device for simultaneously analyzing multiple biomarkers may also include: a cartridge mounting part moving part, which is connected to the cartridge mounting part (210) and moves the cartridge mounting part (210) so that at least one well of the cartridge (100) and the tip connecting part (220) are aligned with each other; and a tip connecting part moving part (260), which is connected to the tip connecting part (220) and moves the tip connecting part (220) on a predetermined moving trajectory.

[0012] The device for simultaneously analyzing multiple biomarkers may also include: a tip connection part mounting base (270), in which the tip connection part (220) is mounted; and a mounting base support part (280), wherein the mounting base support part (280) is mounted on a second moving track (281), wherein the second moving track (281) has one end extending in a vertical direction and is connected to the outer side of the tip connection part mounting base (270), wherein the tip connection part moving part (260) includes: a first tip connection part moving part (261), wherein the first tip connection part moving part (261) is mounted on the tip connection part mounting base The tip coupling portion mounting base (270) is mounted on the tip coupling portion mounting base (270) and provides a driving force to rotate the tip coupling portion mounting base (270) between a position in which a tip coupling portion (220) mounted on the tip coupling portion mounting base (270) is aligned in a vertical direction with at least one well of the cartridge (100) and a position in which the tip coupling portion (220) is aligned in a vertical direction with an optical path on which an optical unit (230) irradiates light; and a second tip coupling portion moving portion (262) is mounted on the mounting base supporting portion (280) and provides a driving force to vibrate the tip coupling portion mounting base (270) in a vertical direction with a predetermined amplitude.

[0013] The cartridge mounting portion moving part can move the cartridge mounting portion (210) in a direction perpendicular to the vertical direction.

[0014] The cartridge (100) may further include: a tip well (102) into which the tip (110) is inserted; and at least one absorption pad well (104) in which an absorption pad (104a) is installed; at least one washing well (106, 107, 108, 109) in which a washing solution is contained; and a punching tip well (103) into which a punching tip (120) for punching holes in the absorption pad well (104), the reaction well (105) and the sealing portions (104b, 105b, 106b, 107b, 108b, 109b) of the at least one washing well (106, 107, 108, 109) is inserted.

[0015] The tip coupling portion (220) may be selectively coupled to the tip (110) or the punch tip (120).

[0016] At least one binding material that specifically binds to a detection target substance different from the detection target substance (T) may also be coupled to the lower surface of the tip (110) coupled to the second binding material (111).

[0017] The device for simultaneously analyzing multiple biomarkers may also include a middle part (231), which is installed between the optical unit (230) and the tip connecting part mounting base (270) and has at least one through hole (231a) formed in the optical path on which the optical unit (230) irradiates light.

[0018] The processing unit may determine whether a plurality of detection target substances (T) exist in the sample (S) using the number of combinations of intensities of light received by the optical unit (230) for different detection target substances.

[0019] The processing unit (240) can determine whether the plurality of detection target substances (T) exist in the sample (S) using the number of combinations of positions of the binding material coupled to the lower surface of the tip (110) and the intensity of light received at these positions.

[0020] The processing unit (240) can determine the amount of multiple detection target substances (T) contained in the sample (S) using the number of combinations of positions of the binding material coupled to the lower surface of the tip (110) and the intensity of light received at these positions.

[0021] The cartridge mounting portion (210) may include a plurality of seating portions (211) in which the cartridge (100) is mounted, the plurality of seating portions (211) being arranged parallel to each other, and the tip connection portion (220) may be formed one-to-one in a number matching the number of the seating portions (211).

[0022] According to another aspect of the present disclosure, a method for simultaneously analyzing multiple biomarkers using the apparatus for simultaneously analyzing multiple biomarkers of claim 5 is provided, the method for simultaneously analyzing multiple biomarkers comprising: (a) injecting a sample (S) into a sample well (101) of a cartridge (100); (b) aligning the tip well (102) of the cartridge (100) and the tip coupling portion (220) in a straight line in a vertical direction; (c) coupling the tip coupling portion (220) to the tip (110); (d) aligning the sample well (101) of the cartridge (100) with the tip connection portion (220) in a straight line in the vertical direction and immersing the tip (110) into the sample (S) contained in the sample well (101); (e) aligning the reaction well (105) of the cartridge (100) with the tip connection portion (220) in a straight line in the vertical direction and immersing the tip (110) into the reaction sample (105c) contained in the reaction well (105); (f) aligning at least (g) aligning one of the washing wells (106, 107, 108, 109) with the tip coupling portion (220) in a straight line in the vertical direction and immersing the tip (110) into the washing solution contained in the one washing well; (h) aligning the absorption pad well (104) of the cartridge (100) with the tip coupling portion (220) in a straight line in the vertical direction and immersing the tip (110) into the absorption pad (104a) disposed in the absorption pad well (104); and (h) rotating the tip coupling portion The invention further comprises a base (270) for mounting the optical unit (230) so that the optical path along which the optical unit (230) irradiates light is aligned in a straight line with the tip (110); (i) irradiating light to the tip (110) using the optical unit (230) and receiving light generated from the tip (110) through the optical unit (230); and (j) determining whether a detection object substance (T) is present in the sample (S) using the intensity of the light received by the optical unit (230), wherein the determination is performed by using the processing unit (240).

[0023] The method for simultaneously analyzing multiple biomarkers may further include, after (a) and before (b): (a1) aligning the punching tip well (103) of the cartridge (100) with the tip connecting portion (220) in a straight line in the vertical direction; (a2) connecting the tip connecting portion (220) to the punching tip (120); (a3) ​​aligning each of the absorption pad well (104), the reaction well (105) and at least one washing well (106, 107, 108, 109) of the cartridge (100) with the tip connecting portion (220) in a straight line in the vertical direction and punching the sealing portion (104b) of the absorption pad well (104), the sealing portion (105b) of the reaction well (105) and the sealing portion (106b, 107b, 108b, 109b) of at least one washing well (106, 107, 108, 109).

[0024] Any one or more of (e), (f) and (g) may further include vibrating the tip coupling portion (220) in a vertical direction at a predetermined amplitude while being immersed in the well of the cartridge (100). BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The above and other features and advantages of the present invention will become more apparent by describing in detail exemplary embodiments thereof with reference to the accompanying drawings, in which:

[0026] Figure 1 and 2 is a view for describing a cartridge installed on an apparatus for simultaneously analyzing a plurality of biomarkers according to one embodiment of the present disclosure;

[0027] Figure 3 and 4 is a perspective view illustrating an apparatus for simultaneously analyzing a plurality of biomarkers according to one embodiment of the present disclosure;

[0028] Figure 5 Is used to describe Figure 3 A diagram of a cartridge installation portion of an apparatus for simultaneously analyzing multiple biomarkers;

[0029] Figure 6 Is used to describe Figure 3 a view of a tip coupling portion of an apparatus for simultaneously analyzing multiple biomarkers and a tip coupling portion mounting base and a mounting base support in which the tip coupling portion is mounted;

[0030] Figure 7 is a view for describing the operational relationship between the barrel mounting portion, the tip coupling portion, the tip coupling portion mounting base, and the mounting base support portion;

[0031] Figure 8 is a view for schematically describing a process of analyzing a sample injected into a cartridge using an apparatus for simultaneously analyzing a plurality of biomarkers according to an embodiment of the present disclosure;

[0032] Figures 9 to 21 is a diagram for describing a process of analyzing a sample injected into a cartridge using an apparatus for simultaneously analyzing a plurality of biomarkers according to an embodiment of the present disclosure;

[0033] Fig. 22 is a flow chart illustrating a method for simultaneously analyzing multiple biomarkers according to one embodiment of the present disclosure; and

[0034] Figure 23 to Figure 25A view for describing the presence or absence of a biomarker in a sample using light received from an optical unit after irradiating light to a tip in an apparatus for simultaneously analyzing a plurality of biomarkers according to an embodiment of the present disclosure. DETAILED DESCRIPTION

[0035] Hereinafter, an apparatus ( 200 ) for simultaneously analyzing a plurality of biomarkers according to one embodiment of the present disclosure will be described in more detail with reference to the accompanying drawings.

[0036] First, refer to Figure 1 and 2 A cartridge (100) mounted on and analyzed by an apparatus (200) for simultaneously analyzing a plurality of biomarkers is described in detail.

[0037] Figure 1 1 is a view for describing a cartridge ( 100 ) that can be mounted on an apparatus ( 200 ) for simultaneously analyzing a plurality of biomarkers according to an embodiment of the present disclosure.

[0038] Reference Figure 1 According to one embodiment of the present disclosure, the cartridge (100) may include a sample well (101), a tip well (102), a punched tip well (103), an absorption pad well (104), a reaction well (105), a first wash well (106), a second wash well (107), a third wash well (108) and a fourth wash well (109).

[0039] The sample (S) of the analyte is injected into the sample well (101), and the sample (S) may be a blood sample of the object to be analyzed, but the sample (S) may be extracted from the person to be tested, such as sweat, saliva, nasal mucus, sputum, urine, feces, carrier, etc. The embodiments of the present disclosure are not limited thereto, as long as the sample (S) can contain the detection object substance (T) that can determine whether the detection object substance (T) is present in the sample (S).

[0040] The tip (110) is inserted into the tip well (102).

[0041] The front end of the tip (110) includes a surface on which a binding material that specifically binds to a detection target substance (T) is dotted by sol-gel. Specific details about the sol-gel embellishment of the bonding material include the contents of the applicant's prior patent applications KR10-2011-0039535 (April 27, 2011), PCT-KR2011-003105 (April 27, 2011), KR10-2006-0008926 (January 27, 2006), PCT-KR2007-000390 (January 23, 2007), KR10-2013-0047564 (April 29, 2013), PCT-KR2013-003657 (April 26, 2013) and KR10-2018-0125995 (October 22, 2018), and the contents of the above patent applications are included in the contents of the present patent application as a whole.

[0042] The tip (110) is detachably inserted into the tip well (102), and a component capable of binding to a detection object substance (biomarker) contained in a target sample (S) by an immunological or non-immunological method is coupled to the lower surface of the tip (110) using sol-gel dotting. At this time, the binding material coupled to the lower surface of the tip (110) may be an antigen, an antibody, or an aptamer, but the embodiments of the present disclosure are not specifically limited thereto, as long as the binding material is a material capable of specifically binding to the detection object substance.

[0043] The binding material connected to the lower surface of the tip (110) is, for example, anti-HIV1 / 2 detection antigen, anti-HCV detection antigen, HBsAg detection antibody, influenza A antibody, influenza B antibody, RSV antibody, SARS-CoV-2 antibody, SARS-CoV-2 antigen, CA19-9 antibody, AFP antibody, CEA antibody, PSA antibody, CA125 antibody, CA15-3 antibody, CA242 antibody, CYFRA21-1 antibody, SCCA antibody, NSE antibody, HE4 antibody, canine-based CDV, CPV, CPIV, CAV-2 and CCoV, cat-based FCV, FPV, FVR (FHV), FIP (FCoV) and feline Chl. (Chl. Felis), etc., but the embodiments of the present disclosure are not limited to this.

[0044] Figure 1A state is shown in which a second binding material (111) as a CA19-9 antibody that specifically binds to CA19-9, a third binding material (112) as an AFP antibody that specifically binds to AFP, a fourth binding material (113) as a CEA antibody that specifically binds to CEA, a fifth binding material (114) as a PSA antibody that specifically binds to PSA, and a sixth binding material (115) as a CA125 antibody that specifically binds to CA125 are connected to the lower surface of a tip (110) using sol-gel dotting (a 5×3 arrangement is shown). In addition, a reference point marker can be formed on the lower surface of the tip (110). As shown in FIG. Figure 1 and 23 As shown, the reference point marker may be formed outside the arrangement of the second to sixth bonding materials (111 to 115), and when light is irradiated by the optical unit (230), a light signal of predetermined intensity may be generated. The intensity of the light signal generated at the position where the reference point marker is formed is set as the reference light intensity, and is compared with the intensity of the light signal generated at the position where other bonding materials are formed later, so that analysis can be performed at the position where the corresponding bonding material is formed.

[0045] One or more binding materials may be connected to the lower surface of the tip (110). Moreover, a single type of binding material or multiple types of binding materials may be connected to the lower surface of the tip (110). Specifically, the binding materials connected to the lower surface of the tip (110) are arranged in a column or a row for each type of binding material, and the arrangement of all binding materials is 2×2, 3×3, 4×4, 5×5, etc. However, the embodiments of the present disclosure are not limited to this. That is, the shape of the arrangement is not limited, as long as all binding materials are connected to the lower surface of the tip (110) and can be combined with the detection object substance included in the sample (S) through the support of the sample (S). In another embodiment of the present disclosure, in order to minimize the error of the analytical method through a triplet test, a binding material can be connected to the lower surface of the tip (110) in an arrangement of 3 rows of each binding material.

[0046] Additionally, in another embodiment of the present disclosure, the binding material coupled to the lower surface of the tip (110) may include two or more selected from the group consisting of influenza A antibodies, influenza B antibodies, RSV antibodies, and SARS-CoV2 antibodies.

[0047] In addition, in another embodiment of the present disclosure, the binding material coupled to the lower surface of the tip (110) may include two or more selected from the group consisting of CA19-9 antibody, AFP antibody, CEA antibody, PSA antibody and CA125 antibody (see Figure 1 ).

[0048] In addition, in another embodiment of the present disclosure, the binding material coupled to the lower surface of the tip (110) may include two or more selected from the group consisting of CA15-3 antibody, CA242 antibody, CYFRA21-1 antibody, SCCA antibody, NSE antibody and HE4 antibody.

[0049] The detection target substance (biomarker) that specifically binds to the binding material connected to the lower surface of the tip (110) may be, for example, nucleic acid, peptide, protein, small molecule material, virus, influenza virus or cell. However, the embodiments of the present disclosure are not limited thereto.

[0050] At the rear end of the tip (110), a tip connection hole (110a) for connection with a tip connection part (220) described later may be formed at the rear end of the tip (110), and the tip connection part (220) is inserted into the tip connection hole (110a), so that connection can be made between the tip connection part (220) and the tip (110).

[0051] The punch tip (120) is inserted into the punch tip well (103).

[0052] The punching tip (120) is removably inserted into the punching tip well (103), and the lower surface of the punching tip (120) is provided with a sharp tip, so that the punching tip (120) is configured to punch (perforate) at least one of the sealing portions (104b) covering the upper portion of the absorption pad well (104), the sealing portion (105b) covering the upper portion of the reaction well (105), the sealing portion (106b) covering the upper portion of the first washing well (106), the sealing portion (107b) covering the upper portion of the second washing well (107), the sealing portion (108b) covering the upper portion of the third washing well (108), and the sealing portion (109b) covering the upper portion of the fourth washing well (109).

[0053] Before being installed on an apparatus (200) for simultaneously analyzing multiple biomarkers and performing analysis, the sample well (101), other wells (104) to (109) other than the sample well (101), the tip well (102) and the punching tip well (103) are sealed by sealing portions (104b) to (109b) to prevent degradation or performance degradation due to reaction with external moisture and air, and analysis of the sample (S) can be performed by punching the sealing portion using a punching tip (120).

[0054] A punching tip coupling hole (120a) for coupling with the tip coupling part (220) is formed at the rear end of the punching tip (120), and the tip coupling part (220) is inserted into the punching tip coupling hole (120a), so that coupling between the tip coupling part (220) and the punching tip (120) can be formed.

[0055] The absorbent pad well (104) is provided with an absorbent pad (104a). A sponge may be used as the absorbent pad (104a). However, if the absorbent pad (104a) is made of a material capable of absorbing moisture, the material of the absorbent pad (104a) is not particularly limited thereto.

[0056] The absorption pad well (104) is a portion into which the tip (110) is inserted after supporting the sample (S), supporting the reaction sample (105c) and the washing solution supporting the tip (110), and since the tip (110) is inserted into the absorption pad well (104), moisture or other unreacted biomaterials remaining on the lower surface of the tip (110) are removed. Therefore, when light is irradiated by the optical unit (230), clearer light receiving sensitivity can be achieved.

[0057] The reaction sample (105c) is stored in the reaction well (105). The reaction sample (105c) includes a first binding material (105a) that specifically binds to the detection object substance (T), similar to the binding material bound to the lower surface of the tip (110). A marker (R) is bound to the first binding material (105a), and the marker (R) may include a radioactive isotope, a fluorescent dye, or another type of marking material. When the marker (R) is connected to the first binding material (105a), the optical unit (230) irradiates light to the tip (110), and the intensity of the light generated by the marker (R) can be used to determine the presence of the detection object substance (T) included in the sample (S) and the amount of the detection object substance (T) included in the sample (S). A detailed description thereof will be provided later.

[0058] Washing solutions (106a) to (109a) are stored in washing wells (106) to (109), respectively. The washing well may be one, but may be provided in two or more, and is not particularly limited thereto. Figure 1 A cartridge (100) is shown having four wash wells (106) to (109).

[0059] The washing solutions (106a) to (109a) may include distilled water and buffer solutions other than distilled water, depending on the characteristics of the detection object substance (T) to be detected, and different types of distilled water and buffer solutions other than distilled water may be stored in different washing wells (106) to (109), respectively.

[0060] In one embodiment of the present disclosure, the buffer solution other than distilled water may include a PBS-based solution, more specifically, Na2HPO4, KH2PO4, NaCl, C 58 H 11 -O 26 A mixed solution of a solution (Tween-20) and a 5-chloro-2-methyl-1,2-thiazol-3-one solution (Proclin 300). The cartridge (100) according to one embodiment of the present disclosure includes a buffer other than distilled water, so that unreacted biological materials remaining on the lower surface of the tip (110) can be more effectively removed, thereby improving the accuracy of the analysis.

[0061] A label capable of specifying the sample from which the sample (S) has been collected can be printed on the outer surface of the cartridge (100), and the sample from which the sample (S) has been collected can be specified for analysis by a process of identifying the label. Therefore, the analysis result of the cartridge (100) can be processed, stored, and output by matching the sample specified by the label identification.

[0062] Next, we will refer to Figures 3 to 7 A device (200) for simultaneously analyzing multiple biomarkers according to one embodiment of the present disclosure is described in detail.

[0063] The device (200) for simultaneously analyzing multiple biomarkers is equipped with the above-mentioned cartridge (100), analyzes the sample (S) injected into the cartridge (100), and judges / determines whether the detection target substance (T) exists in the sample (S).

[0064] refer to Figure 3 and 4 The device (200) for simultaneously analyzing multiple biomarkers includes a cartridge mounting portion (210), a tip connecting portion (220), an optical unit (230), a processing unit (240), a cartridge mounting portion moving portion and a tip connecting portion moving portion (260), a tip connecting portion mounting base (270) and a mounting base supporting portion (280).

[0065] The cartridge mounting portion (210) is formed with a seating portion (211) on which the cartridge (100) is placed. The number of the seating portions (211) formed in the cartridge mounting portion (210) may be one, but may also be two or more, and Figure 2 An embodiment in which six seating portions (211) are formed is shown.

[0066] One cartridge (100) can be installed in one seating portion (211), and in the case of a cartridge mounting portion (210) in which a plurality of seating portions (211) are formed, a plurality of cartridges (100) can be analyzed at once. A detailed description thereof will be provided later.

[0067] The cartridge mounting portion (210) can be moved by the cartridge mounting portion moving part.

[0068] Specifically, the cartridge mounting portion (210) is installed in a manner that is separated by a predetermined distance from the bottom surface (201) of the device (200) for simultaneously analyzing multiple biomarkers, and a cartridge mounting portion support (212) is formed between the bottom surface (201) and the cartridge mounting portion (210) so as to support the cartridge mounting portion (210).

[0069] A first moving track (201a) for movement of the cartridge mounting portion (210) is formed on the bottom surface (201), and the cartridge mounting portion support (212) is coupled to the first moving track (201a) so as to move along the first moving track (201a).

[0070] In addition, the barrel mounting part support (212) is connected to the rotating shaft of the first driving motor (213) that provides driving force for the movement of the barrel mounting part (210), and when the first driving motor (213) rotates, the barrel mounting part support (212) can move along the first moving track (201a).

[0071] The moving direction of the barrel mounting part (210) according to the rotation of the first driving motor (213) may be a direction parallel to the ground. However, the embodiments of the present disclosure are not limited thereto, and the barrel mounting part (210) may also move in the left-right direction while forming a predetermined angle relative to the ground.

[0072] The tip coupling portion (220) is coupled to the above-mentioned tip (110) and the punching tip (120). More specifically, the tip coupling portion (220) is inserted into a tip coupling hole (110a) formed at the rear end of the tip (110), thereby being coupled to the tip (110). In addition, the tip coupling portion (220) is inserted into a punching tip coupling hole (120a) formed at the rear end of the punching tip (120), thereby being coupled to the punching tip (120).

[0073] The tip coupling part (220) is installed in such a manner that a portion of the tip of the tip coupling part (220) is exposed to the outside, and the other portion which is not exposed is inserted into the tip coupling part installation base (270).

[0074] The mounting base support portion (280) is coupled to the outer side of the tip coupling portion mounting base (270).

[0075] In an apparatus (200) for simultaneously analyzing a plurality of biomarkers, a second moving track (281) for moving a mounting base support portion (280) extends in a vertical direction, and the mounting base support portion (280) is connected to the second moving track (281) so as to move along the second moving track (281).

[0076] In addition, the mounting base support part (280) is connected to the rotating shaft of the second driving motor (282) that provides driving force for the movement of the mounting base support part (280), and when the second driving motor (282) rotates, the mounting base support part (280) can move along the second moving track (281).

[0077] When the mounting base support part (280) moves, the tip coupling part mounting base (270) coupled to the mounting base support part (280) and the tip coupling part (220) mounted on the tip coupling part mounting base (270) also move together, and the direction in which the mounting base support part (280) moves according to the rotation of the second driving motor (282) may be a direction perpendicular to the ground. However, the embodiments of the present disclosure are not limited thereto, and the mounting base support part (280) may also be moved in a vertical direction while forming a predetermined angle relative to the ground.

[0078] That is, the moving direction of the tip coupling portion (220) may be perpendicular to the moving direction of the cartridge (100).

[0079] A tip coupling portion moving part (260) for moving the tip coupling portion (220) may be formed in the mounting base supporting portion (280). Figure 6 The tip connection part moving portion (260) may be formed on a side of the mounting base support portion (280), and may include a first tip connection part moving portion (261) and a second tip connection part moving portion (262).

[0080] The first tip coupling portion moving part (261) is a portion for rotational movement of the tip coupling portion (220).

[0081] The first tip coupling portion moving part (261) is coupled to the tip coupling portion mounting base (270) while passing through the mounting base supporting part (280) from both sides of the mounting base supporting part (280).

[0082] The first tip connection part moving parts (261) formed on both sides of the mounting base supporting part (280) each include a third driving motor (not shown), and as the third driving motor (not shown) rotates, the tip connection part mounting base (270) connected thereto can rotate.

[0083] When the tip coupling part mounting base (270) rotates, the tip coupling part (220) mounted therein also rotates together, and more specifically, the tip coupling part (220) can rotate from a position perpendicular to the ground to a position parallel to the ground.

[0084] When the tip coupling portion (220) is rotated to a position parallel to the ground by the rotation of the third driving motor (not shown), the lower surface of the tip (110) coupled to the tip coupling portion (220) is located on the optical path on which the optical unit (230) irradiates light. Therefore, since the analysis is performed in a state where the lower surface of the tip (110) is exposed to the outside air rather than in a state where it is supported on a sample or a reagent, there is no interference from external factors such as contamination and residues. Therefore, a more accurate optical signal can be detected.

[0085] The second tip coupling portion moving part (262) is a portion for vibrating the tip coupling portion (220) in a vertical direction.

[0086] The second tip coupling part moving part (262) is installed on one side of the mounting base support part (280). To this end, a mounting groove (283) having a length longer than that of the second tip coupling part moving part (262) is formed in the mounting base support part (280).

[0087] The second tip connection part moving part (262) is connected to the rotating shaft of the fourth drive motor (263), which is installed to face the mounting base support part (280) interposed between it and the second tip connection part moving part, and when the fourth drive motor (263) rotates, the second tip connection part moving part (262) can vibrate in the vertical direction along the mounting groove (283).

[0088] In other words, the vertical length of the mounting groove (283) is longer than the vertical length of the second tip connecting portion moving portion (262), and the mounting base support portion (280) is able to vibrate in the vertical direction using the difference between the vertical length of the mounting groove (283) and the vertical length of the second tip connecting portion moving portion (262) as an amplitude.

[0089] When the mounting base support part (280) vibrates in the vertical direction, the tip coupling part mounting base (270) coupled to the mounting base support part (280) and the tip coupling part (220) mounted in the tip coupling part mounting base (270) vibrate in the vertical direction.

[0090] By vibrating in the vertical direction, the tip (110) can vibrate in the vertical direction in the well with a predetermined amplitude. Therefore, the reaction between the binding material connected to the lower surface of the tip (110) and the detection object substance, the reaction between the detection object substance (T) and the binding material in the reaction sample (105c), and the washing of the unreacted biological material remaining on the lower surface of the tip (110) can be carried out more effectively.

[0091] The optical unit (230) includes an irradiator for irradiating light to the outside and a receiving part for receiving light entering the inside.

[0092] The tip coupling portion (220) is rotated by the first tip coupling portion moving part (261), and the tip (110) coupled to the tip coupling portion (220) is rotated together.

[0093] At this time, the lower surface of the tip (110) is located on the optical path on which the optical unit (230) irradiates light. For example, an adhesive bonded in the order of a first bonding material, a detection object substance, and a second bonding material is located on the lower surface of the tip (110). Here, a marker (R) such as a radioactive isotope or a fluorescent material is bonded to the first bonding material, and the first bonding material generates a specific light signal according to the type of the marker (R) by irradiation of light from the optical unit (230).

[0094] The optical unit (230) can receive a specific optical signal, and the processing unit (240) determines whether the detection target substance (T) exists in the sample (S) and the amount of the detection target substance (T) contained in the sample (S) according to the intensity of the received optical signal.

[0095] A middle portion (231) having a through hole (231a) formed therethrough is further formed between the optical unit (230) and the tip coupling portion (220).

[0096] The number of through holes (231a) may be the same as the number of the seating portions (211) formed in the cartridge mounting portion (210), and Figure 3 2 shows a middle portion (231) in which six through holes (231a) are formed.

[0097] Light irradiated by the optical unit (230) passes through the through hole (231a) of the middle portion (231), and an optical signal generated on the lower surface of the tip (110) according to the irradiation of the light also passes through the through hole (231a) and is received by the optical unit (230).

[0098] The optical unit (230) receives only the optical signal passing through the through hole (231a), and since the received optical signal is used for analysis, interference caused by light incident from the outside can be minimized.

[0099] The optical unit (230) is installed to be separated from the bottom surface (201) by a predetermined distance, and the optical unit support (232) is installed between the bottom surface (201) and the optical unit (230), and the optical unit support (232) can move along the third moving track (233) in a direction parallel to the ground. To this end, the optical unit support (232) is connected to the fifth driving motor (234), so that the optical unit support (232) can move along the third moving track (233) according to the rotation of the fifth driving motor (234).

[0100] More specifically, when the optical unit (230) and the through hole (231a) are aligned with each other so that light irradiated from the optical unit (230) passes through the through hole (231a) formed in the middle part (231), the optical unit (230) is movable. All the tips of the plurality of tips (110) coupled to the plurality of tip coupling parts (220) can be analyzed according to the movement of the optical unit (230).

[0101] The moving direction of the apparatus (200) for simultaneously analyzing a plurality of biomarkers according to one embodiment of the present disclosure is summarized as follows.

[0102] like Figure 3 As shown, if a three-axis coordinate system consisting of an x-axis, a y-axis, and a z-axis is used as an example for description, the barrel mounting portion (210) can move along the y-axis, and the vertical movement of the tip coupling portion mounting base (270) can move along the z-axis, and the optical unit (230) can move along the x-axis. The movement of the barrel mounting portion (210), the vertical movement of the tip coupling portion mounting base (270), and the movement of the optical unit (230) can be perpendicular to each other. However, the embodiments of the present disclosure are not particularly limited to this.

[0103] The processing unit (240) determines whether the detection target substance (T) is present in the sample (S) injected into the cartridge (100) using the light received by the optical unit (230) and the amount of the detection target substance (T) contained in the sample (S).

[0104] The processing unit (240) may be a microprocessor (MCU) or a computer having data calculation and processing functions, but is not limited thereto, as long as the processing unit (240) has data calculation and processing functions.

[0105] Will be Figures 23 to 25 Take this as an example for description.

[0106] When the detection target substance (T) exists in the sample (S), the detection target substance (T) specifically binds to the binding material formed on the tip (110), and the detection target substance (T) also specifically binds to the binding material contained in the reaction sample (105c).

[0107] Thus, an adhesive is formed in which the first binding material, the detection target substance, and the second binding material are bound in this order. A marker (R) such as a radioisotope or a fluorescent material is bound to the first binding material, and the first binding material generates a specific light signal by light irradiation.

[0108] When the sample (S) contains a detection object substance (T), the optical unit (230) can receive a specific light signal through light irradiation, and the processing unit (240) can use the positions of different binding materials connected to the lower surface of the tip (110) and the intensity of the light signal at the corresponding position to determine whether the detection object substance (T) exists in the sample (S) and the amount of the detection object substance (T) contained in the sample (S).

[0109] Fig.23 The state in which no optical signal having a predetermined intensity or higher is detected at a position where other binding materials are formed except for a position where a reference point marker is formed is shown. The processing unit (240) can determine that the detection target substance (T) does not exist in the corresponding sample.

[0110] in addition, Fig.24 The state in which a light signal with high intensity is detected at the position where the third binding material (112) as the AFP antibody and the fourth binding material (113) as the CEA antibody are formed and a light signal with low intensity is detected at the position where the fifth binding material (114) as the PSA antibody and the sixth binding material (115) as the CA125 antibody are formed is shown. In addition, Fig.24 The state in which a light signal having a very low intensity is detected at the position where the second binding material (111) as the CA19-9 antibody is formed is shown.

[0111] The processing unit (240) can determine that the detection target substances CA19-9, AFP, CEA, PSA and CA125 exist in the corresponding sample, but can determine that AFP and CEA are more than PSA and CA125, and CA19-9 is less than PSA and CA125.

[0112] exist Fig.25 In the embodiment, a light signal with a low intensity is detected at the position where the third binding material (112) as an AFP antibody and the fourth binding material (113) as a CEA antibody are formed, and a light signal with a very low intensity is detected at the position where the fifth binding material (114) as a PSA antibody and the sixth binding material (115) as a CA125 antibody are formed. In addition, almost no light signal is detected at the position where the second binding material (111) as a CA19-9 antibody is formed. The processing unit (240) can determine that CA19-9 is not present in the corresponding sample, and can determine that AFP, CEA, PSA and CA125 are present, but the processing unit (240) can determine that AFP and CEA are more than PSA and CA125. In addition, the processing unit (240) can use the received light intensity to determine Fig.25 The amount of AFP and CEA in the corresponding samples is less than Fig.24 The amounts of AFP and CEA in the corresponding samples.

[0113] As such, in a device (200) for simultaneously analyzing multiple biomarkers according to an embodiment of the present disclosure, multiple biomarkers are connected to one tip (110), and optical signals at locations where the corresponding biomarkers are connected to the tip (110) are analyzed, thereby determining whether the biomarkers exist in a sample (S).

[0114] In addition, in an apparatus (200) for simultaneously analyzing multiple biomarkers according to an embodiment of the present disclosure, multiple cartridges (100) can be placed in one cartridge mounting portion (210), and the presence or absence of biomarkers in samples (S) of multiple samples can also be determined in one analysis operation.

[0115] In the following, reference will be made to Figures 9 to 21 A method for simultaneously analyzing multiple biomarkers using an apparatus (200) for simultaneously analyzing multiple biomarkers is described in detail.

[0116] First, the cartridge (100) is mounted on the seating portion (211) of the cartridge mounting portion (210).

[0117] Next, the sample (S) is injected into the sample well (101) of the cartridge (100) ( Fig.10 ).

[0118] Next, the first drive motor (213) rotates so that the cartridge mounting portion (210) moves along the y-axis direction, and the punching tip well (103) and the tip coupling portion (220) of the cartridge (100) are aligned with each other in a straight line in the vertical direction (in the z-axis direction) ( Fig.11 ).

[0119] Next, the second driving motor (282) rotates so that the mounting base support portion (280) moves downward along the z-axis direction, and the punching tip (120) disposed in the punching tip well (103) and the punching tip coupling portion (220) are coupled to each other ( Fig.12 ).

[0120] Next, the first drive motor (213) rotates so that the cartridge mounting portion (210) moves along the y-axis direction, and each of the absorption pad well (104), reaction well (105) and one or more washing wells (106) to (109) of the cartridge (100) is aligned in a straight line with the tip connecting portion (220) in the vertical direction.

[0121] Next, the second drive motor (282) rotates so that the mounting base support portion (280) moves downward along the z-axis direction, and each of the sealing portions (104b, 105b, 106b, 107b, 108b, 109b) for sealing the absorption pad well (104), the reaction well (105) and the one or more washing wells (106) to (109) is perforated by the punching tip (120) ( Fig.13 ).

[0122] Next, the tip coupling portion (220) is separated from the punch tip (120), and the first drive motor (213) is rotated to move the cartridge mounting portion (210) along the y-axis direction to combine the tip well (102) and the tip. The tip coupling portion (220) and the tip (110) are aligned in the vertical direction ( Fig.14 ).

[0123] Next, the second driving motor (282) rotates, so that the mounting base support portion (280) moves downward along the z-axis direction, and the tip (110) and the tip coupling portion (220) disposed in the tip well (102) are coupled to each other ( Fig.15 ).

[0124] Next, the first driving motor (213) rotates so that the cartridge mounting portion (210) moves along the y-axis direction, and the sample well (101) and the tip coupling portion (220) are aligned in a straight line in the vertical direction.

[0125] Next, the second driving motor (282) rotates, so that the mounting base supporting portion (280) moves downward along the z-axis direction, and the tip (110) connected to the tip connecting portion (220) is supported on the sample (S) in the sample well (101) ( Fig.16 ).

[0126] In this process, the binding material that can specifically bind to the detection target substance (T) and is coupled to the lower surface of the tip (110) binds to the detection target substance (T) contained in the sample (S).

[0127] In addition, during this process, the fourth driving motor (263) rotates, and therefore, the mounting base support portion (280), the tip coupling portion mounting base (270) and the tip coupling portion (220) vibrate in a vertical direction with a predetermined amplitude.

[0128] That is, as the lower surface of the tip (110) vibrates in the vertical direction when supported on the sample (S), the binding material coupled to the lower surface of the tip (110) and the detection object substance (T) contained in the sample (S) can be effectively combined. However, the vertical vibration movement of the tip coupling part (220) is not necessary, and the process can be removed.

[0129] Next, the first driving motor (213) rotates so that the cartridge mounting portion (210) moves along the y-axis direction, and the reaction well (105) and the tip coupling portion (220) are aligned in a straight line in the vertical direction.

[0130] Next, the second driving motor (282) rotates, so that the mounting base support portion (280) moves downward along the z-axis direction, and the lower surface of the tip (110) is supported and disposed on the reaction sample (105c) in the reaction well (105). Fig.17 ).

[0131] In this process, the first binding material (105a) contained in the reaction sample (105c) specifically binds to the binding material-detection target substance adhesive bonded to the lower surface of the tip (110) (more specifically, specifically binds to the detection target substance).

[0132] In this process, the fourth driving motor (263) rotates, and therefore, the mounting base support (280), the tip coupling portion mounting base (270) and the tip coupling portion (220) vibrate in the vertical direction with a predetermined amplitude ( Fig.18 ).

[0133] That is, as the lower surface of the tip (110) vibrates in the vertical direction when supported on the reaction sample (105c), the binding material-detection object substance adhesive connected to the lower surface of the tip (110) can be effectively combined with the first binding material (105a) contained in the reaction sample (105c).

[0134] Next, the first drive motor (213) rotates so that the cartridge mounting portion (210) moves along the y-axis direction, and any one of the one or more washing wells (106) to (109) and the tip connection portion (220) are aligned in a straight line in the vertical direction.

[0135] Next, the second drive motor (282) rotates, so that the mounting base support portion (280) moves downward along the z-axis direction, and the lower surface of the tip (110) is supported in the washing solution (106a) provided in the washing well (106) ( Fig.19 ).

[0136] In this process, the unreacted biomaterial remaining on the lower surface of the tip (110) can be washed by the washing solution (106a), and as a result of the washing, for example, only the first binding material-detection object substance-second binding material adhesive can be bound to the lower surface of the tip (110). Therefore, the problem of inaccurate analysis caused by interference of the optical signal due to the unreacted biomaterial is prevented.

[0137] In this process, the fourth driving motor (263) rotates, and thus the mounting base support portion (280), the tip coupling portion mounting base (270), and the tip coupling portion (220) vibrate in a vertical direction with a predetermined amplitude.

[0138] That is, as the lower surface of the tip (110) vibrates in the vertical direction while being supported in the washing solution (106a), unreacted biomaterials remaining on the lower surface of the tip (110) can be effectively washed.

[0139] Next, the first drive motor (213) rotates, causing the cartridge mounting portion (210) to move along the y-axis direction so that the absorbent pad well (104) and the tip coupling portion (220) are aligned in a straight line in the vertical direction.

[0140] Next, the second drive motor (282) rotates, causing the mounting base support portion (280) to move downward along the z-axis direction, and the lower surface of the tip (110) is inserted into the absorbent pad (104a) disposed in the absorbent pad well (104). Fig. 20 ).

[0141] In this process, moisture and unreacted biomaterial remaining on the lower surface of the tip (110) may be separated from the tip (110).

[0142] Next, as the third driving motor (not shown) rotates, the tip coupling portion mounting base (270) rotates, and thus the lower surface of the tip (110) is located on an optical path on which the optical unit (230) irradiates light.

[0143] Next, the optical unit (230) irradiates light to the lower surface of the tip (110), and a specific light signal generated when the irradiated light reaches the marker (R) is received by the optical unit (230) ( Fig.21 ).

[0144] Next, the processing unit (240) can use the intensity of the light signal received by the optical unit (230), for example, in response to the portion of the tip (110) having an intensity greater than or equal to a predetermined intensity level, to determine the presence of the detection object substance (T), and can further determine the amount of the detection object substance (T).

[0145] When using the device for simultaneously analyzing multiple biomarkers disclosed herein, the analysis error caused by components other than the sample can be minimized by connecting the detection target substance to the lower end of the tip and moving it, thereby reducing the time and cost required for the detection process of the detection target substance.

[0146] In addition, compared with the analysis device according to the prior art that detects the light signal generated in the well of the barrel, in the analysis device according to the present disclosure, the light signal generated at the tip outside the well of the barrel can be detected. Therefore, in the analysis device according to the present disclosure, since the light signal is less or not completely disturbed by external factors such as contamination and residues, a more accurate light signal can be detected.

[0147] According to the above description, those skilled in the art to which the present disclosure belongs will understand that the present disclosure can be implemented in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the above embodiments are exemplary and not restrictive in all aspects. The scope of the present disclosure should be interpreted as being included within the scope of the present disclosure, rather than the above detailed description, including all changes or modifications derived from the meaning and scope of the following claims and their equivalents.

[0148] Reference numerals list

[0149] R: marker

[0150] S: Sample

[0151] T: Detection target substance

[0152] 100: Barrel

[0153] 101: Sample Well

[0154] 102: Tip Well

[0155] 103: Punching Tip Well

[0156] 104: Absorption pad well

[0157] 105: Reaction well

[0158] 105a: first bonding material

[0159] 105c: Reaction sample

[0160] 106: First washing well

[0161] 107: Second washing well

[0162] 108: Third washing well

[0163] 109: Fourth Washing Well

[0164] 110: Cutting Edge

[0165] 110a: Tip connection hole

[0166] 111: Second bonding material

[0167] 112: Third bonding material

[0168] 113: Fourth binding material

[0169] 114: Fifth binding material

[0170] 115: Sixth binding material

[0171] 120: Punching tip

[0172] 120a: Punching tip connection hole

[0173] 200: Equipment for simultaneous analysis of multiple biomarkers

[0174] 201: Bottom

[0175] 201a: First moving track

[0176] 210: Barrel installation part

[0177] 211: Resettlement section

[0178] 212: Barrel mounting part support

[0179] 213: First drive motor

[0180] 220: Tip connection part

[0181] 230: Optical unit

[0182] 231: Middle part

[0183] 231a:Through hole

[0184] 232: Optical unit support

[0185] 233: The third moving track

[0186] 234: Fifth drive motor

[0187] 240: Processing unit

[0188] 260: Tip connection part moving part

[0189] 261: First tip connection part moving part

[0190] 262: Second tip connection part moving part

[0191] 263: Fourth drive motor

[0192] 270: Tip connection part mounting base

[0193] 280: Install the base support part

[0194] 281: Second moving track

[0195] 282: Second drive motor

[0196] 283: Installation groove

Claims

1. A device for simultaneously analyzing multiple biomarkers, comprising: a cartridge installation portion (210), in which a cartridge (100) is installed, wherein the cartridge (100) comprises at least one well and a tip (110), wherein the at least one well comprises a sample well (101) and a reaction well (105), a sample (S) containing a detection object substance (T) is injected into the sample well (101), the reaction well (105) contains a first binding material (105a), the first binding material (105a) can specifically bind to the detection object substance (T) and a marker (R) is connected to the first binding material (105a), the tip (110) is inserted into the at least one well and a second binding material (111) that can specifically bind to the detection object substance (T) is connected to one end of the tip (110); a tip coupling portion (220) coupled to the tip (110) and capable of moving on a predetermined movement trajectory when coupled to the tip (110); an optical unit (230) that irradiates light to the tip (110) and receives light generated from the tip (110) according to the irradiation of light; a processing unit (240) for determining whether the detection target substance (T) exists in the sample (S) according to a predetermined method using the light received by the optical unit (230), a cartridge mounting portion moving portion, the cartridge mounting portion moving portion being coupled to the cartridge mounting portion (210) and moving the cartridge mounting portion (210) so that the at least one well of the cartridge (100) and the tip coupling portion (220) are aligned with each other; a tip connection part moving part (260), the tip connection part moving part (260) being connected to the tip connection part (220) and causing the tip connection part (220) to move on the predetermined movement trajectory, a tip connection part mounting base (270), wherein the tip connection part (220) is mounted in the tip connection part mounting base (270); and a mounting base support portion (280) mounted on a second moving rail (281) having one end extending in a vertical direction and coupled to an outer side of the tip coupling portion mounting base (270), Wherein, the tip connection part moving part (260) comprises: a first tip coupling portion moving part (261) which is mounted on the tip coupling portion mounting base (270) and provides a driving force so that the tip coupling portion mounting base (270) rotates between a position in which the tip coupling portion (220) mounted on the tip coupling portion mounting base (270) is aligned in a vertical direction with the at least one well of the cartridge (100) and a position in which the tip coupling portion (220) is aligned in a straight line with an optical path along which light is irradiated by the optical unit (230); and A second tip coupling portion moving part (262) is mounted on the mounting base supporting part (280) and provides a driving force so that the tip coupling portion mounting base (270) vibrates in a vertical direction with a predetermined amplitude.

2. The device for simultaneously analyzing multiple biomarkers according to claim 1, wherein: The cartridge mounting portion moving part moves the cartridge mounting portion (210) in a direction perpendicular to the vertical direction.

3. The device for simultaneously analyzing multiple biomarkers according to claim 2, wherein: The barrel (100) further comprises: a tip well (102) in which the tip (110) is inserted; and at least one absorbent pad well (104), wherein an absorbent pad (104a) is installed in the absorbent pad well (104); at least one wash well (106, 107, 108, 109) in which a wash solution is contained; and A punching tip well (103) is inserted into the punching tip well (103) for punching the sealing parts (104b, 105b, 106b, 107b, 108b, 109b) of the absorption pad well (104), the reaction well (105) and the at least one washing well (106, 107, 108, 109).

4. The device for simultaneously analyzing multiple biomarkers according to claim 3, wherein: The tip coupling portion (220) is selectively coupled to the tip (110) or the punch tip (120).

5. The device for simultaneously analyzing multiple biomarkers according to claim 2, wherein: At least one binding material capable of specifically binding to a detection target substance different from the detection target substance (T) is further coupled to the lower surface of the tip (110), on which a second binding material (111) is coupled.

6. The device for simultaneously analyzing multiple biomarkers according to claim 2 further comprises an intermediate part (231), which is installed between the optical unit (230) and the tip connection part mounting base (270) and has at least one through hole (231a), and the at least one through hole is formed on the optical path along which the light is irradiated by the optical unit (230).

7. The device for simultaneously analyzing multiple biomarkers according to claim 5, wherein: The processing unit (240) determines whether a plurality of detection target substances (T) exist in the sample (S) by using the number of combinations of the intensities of the light received by the optical unit (230) for different detection target substances.

8. The device for simultaneously analyzing multiple biomarkers according to claim 7, wherein: The processing unit (240) determines whether the plurality of detection target substances (T) are present in the sample (S) using the number of combinations of positions of the binding material coupled to the lower surface of the tip (110) and the intensity of light received at these positions.

9. The device for simultaneously analyzing multiple biomarkers according to claim 7, wherein: The processing unit (240) determines the amount of the plurality of detection target substances (T) contained in the sample (S) using the number of combinations of positions of the binding material coupled to the lower surface of the tip (110) and the intensity of light received at these positions.

10. The device for simultaneously analyzing multiple biomarkers according to claim 1, wherein: The cartridge installation portion (210) includes a plurality of placement portions (211), the cartridge (100) is installed in the placement portion (211), the plurality of placement portions (211) are arranged parallel to each other, and The tip coupling portions (220) are formed one-to-one in number matching the number of the seating portions (211).

11. A non-therapeutic and non-diagnostic method for simultaneously analyzing multiple biomarkers using the device for simultaneously analyzing multiple biomarkers according to claim 3, wherein the non-therapeutic and non-diagnostic method for simultaneously analyzing multiple biomarkers comprises: (a) injecting a sample (S) into a sample well (101) of a cartridge (100); (b) aligning the tip well (102) of the cartridge (100) and the tip coupling portion (220) in a straight line in the vertical direction; (c) coupling the tip coupling portion (220) to the tip (110); (d) aligning the sample well (101) of the cartridge (100) and the tip coupling portion (220) in a straight line in a vertical direction, and immersing the tip (110) into a sample (S) contained in the sample well (101); (e) aligning the reaction well (105) of the cartridge (100) and the tip coupling portion (220) in a straight line in a vertical direction, and immersing the tip (110) into a reaction sample (105c) contained in the reaction well (105); (f) aligning one of the at least one washing wells (106, 107, 108, 109) of the cartridge (100) with the tip coupling portion (220) in a straight line in a vertical direction, and immersing the tip (110) in a washing solution contained in the one washing well; (g) aligning the absorbent pad well (104) of the cartridge (100) and the tip coupling portion (220) in a straight line in a vertical direction, and immersing the tip (110) into an absorbent pad (104a) disposed in the absorbent pad well (104); (h) rotating the tip coupling portion mounting base (270) so that the light path along which the light is irradiated by the optical unit (230) is aligned in a straight line with the tip (110); (i) irradiating light to the tip (110) using the optical unit (230), and receiving light generated from the tip (110) through the optical unit (230); as well as (j) determining whether or not a detection target substance (T) is present in a sample (S) using the intensity of light received by the optical unit (230), wherein the determination is performed by using a processing unit (240).

12. The non-therapeutic, non-diagnostic method for simultaneously analyzing multiple biomarkers according to claim 11, further comprising after (a) and before (b): (a1) aligning the punching tip well (103) of the cartridge (100) and the tip coupling portion (220) in a straight line in the vertical direction; (a2) coupling the tip coupling portion (220) to the punch tip (120); and (a3) Aligning the absorption pad well (104), the reaction well (105) and each of the at least one washing well (106, 107, 108, 109) of the cartridge (100) in a straight line with the tip connecting portion (220) in the vertical direction, and punching the sealing portion (104b) of the absorption pad well (104), the sealing portion (105b) of the reaction well (105) and the sealing portions (106b, 107b, 108b, 109b) of the at least one washing well (106, 107, 108, 109).

13. The non-therapeutic and non-diagnostic method for simultaneously analyzing multiple biomarkers according to claim 11, wherein: Any one or more of (e), (f) and (g) further includes vibrating the tip coupling portion (220) in a vertical direction at a predetermined amplitude while being immersed in the well of the cartridge (100).

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