Adhesive tape, and application of skin microbial communities and skin metabolites collected from the skin surface of a subject

Abstract

Provided is an adhesive tape for collecting skin resident microorganisms that can increase the amount of skin resident microorganisms collected on the skin surface. The adhesive tape for collecting skin resident microorganisms is used for collecting skin resident microorganisms on the skin surface, and comprises a substrate and an adhesive layer disposed on at least a portion of the substrate surface, wherein the adhesive layer contains an adhesive, wherein the adhesive contains a rubber-based adhesive, and the adhesive force measured in the following manner according to JIS Z 0237:2009 is 10.0 N or more: the test piece is 12 mm wide, the adherend is a bakelite board, the pressing method is to use a 2 kg roller to go back and forth once, the stretching speed is 300 mm / min, and the stretching angle is 90°; and the peeling force measured in the following manner according to JIS Z 0237:2009 is 0.25 N or more: the test piece is 12 mm wide, chemical pulp paper treated with organic silicon is used as the release paper, the stretching speed is 300 mm / min, and the stretching angle is 180°.

Description

Technical Field

[0001] The present invention relates to an adhesive tape for collecting skin resident microorganisms, a method for evaluating the physical condition of a subject, a method for presenting information to a subject, a method for screening substances for improving or preventing the physical condition, and an adhesive tape for collecting skin metabolites. Background Art

[0002] Conventionally, the condition of human skin is evaluated based on indicators such as tension, texture, spots, wrinkles, moisture content, oil content, water loss (barrier function), etc. As evaluation methods, questionnaire surveys, image analysis of the skin surface disclosed in Patent Document 1, etc. (tension, texture, spots, wrinkles, etc.), and measurement using machines (moisture content, oil content, water loss, etc.) are used.

[0003] However, questionnaire surveys are subject to the influence of the subjects' subjective opinions, so it is difficult to obtain objective evaluations. In contrast, image analysis can obtain objective indicators, but the skin quality changes due to whether the face is cleaned, foundation, skin cream, etc., so it is difficult to accurately evaluate the skin condition.

[0004] In addition, it is known that factors affecting skin condition are not limited to the above-mentioned indicators, and there are skin conditions that are difficult to evaluate with the previous technology that relies on the above-mentioned indicators. The demand for technology that can evaluate such skin conditions is increasing. This demand is not limited to skin conditions, but is generally the same for body conditions.

[0005] Therefore, as a technology that can objectively evaluate and evaluate skin conditions that are not easily affected by skin quality changes caused by facial cleaning, etc., the inventors of the present application have developed a method for evaluating the physical condition of a subject, the method comprising the following steps: determining the value of the skin microbial community collected from the skin surface of the subject for the existence ratio of skin-resident microorganisms on the skin surface for which a correlation benchmark with the physical condition has been established, or a parameter based on the existence ratio, and comparing it with the correlation benchmark, wherein the physical condition is the state of the skin of at least one of skin age, oily skin, spots, dullness, sagging, wrinkles, tension of the skin, the state of pores, or a state related to hair, teeth, urine, bones, internal organs, blood, urine or nerves (refer to patent document 2).

[0006] Prior art literature

[0007] Patent Literature

[0008] Patent Document 1: Japanese Patent Application Publication No. 2013-188326

[0009] Patent Document 2: Japanese Patent No. 6046849

[0010] Non-patent literature

[0011] Non-patent document 1: Zouboulis, CC, Stratakis, CA, Chrousos, GP, et al., Metabolism and skin diseases. Rev Endocr Metab Disord 17, 241-246 (2016). Summary of the invention

[0012] Problems to be solved by the invention

[0013] Here, the technology of Patent Document 2 requires collection of skin-resident microorganisms from the skin surface of a test subject.

[0014] As a method for collecting skin resident microorganisms from the skin surface of a subject, there is a wet collection method using a swab (cotton swab). Although this method can well collect skin resident microorganisms on the skin surface, the collection technique is unstable, and there is also a problem that the collected material must be stored in a refrigerated or frozen state in order to preserve it well.

[0015] In this regard, a method of dry collecting skin resident microorganisms from the skin surface using an adhesive tape has also been considered, but this method is difficult to collect skin resident microorganisms on the skin surface well. Since the amount of skin resident microorganisms on the skin surface is small, it is desirable to be able to collect skin resident microorganisms on the skin surface well in order to evaluate with high accuracy.

[0016] Furthermore, if skin metabolites can be collected from the skin surface, it is possible to evaluate the physical condition based on the collected skin metabolites (see Non-Patent Document 1). Therefore, it is also desired to collect skin metabolites on the skin surface well.

[0017] The present invention is made in view of the above-mentioned actual situation in the past. The technical problem of the present invention is to provide an adhesive tape for collecting skin resident microorganisms and an adhesive tape for collecting skin metabolites that can increase the amount of skin resident microorganisms collected on the skin surface, as well as a method for evaluating the physical condition of a subject using them, a method for presenting information to a subject, and a method for screening substances that improve or prevent the physical condition.

[0018] Means for solving problems

[0019] The inventors of the present application have found that the amount of skin-resident microorganisms collected on the skin surface can be increased by providing an adhesive tape for collecting skin-resident microorganisms having an adhesive layer containing a rubber-based adhesive and having specific adhesive force and release force, thereby completing the present invention.

[0020] In addition, the inventors have found that a pressure-sensitive adhesive tape for collecting skin metabolites having a pressure-sensitive adhesive layer containing a rubber-based pressure-sensitive adhesive and having specific pressure-sensitive adhesive force and release force can satisfactorily collect skin metabolites on the skin surface.

[0021] (1) An adhesive tape for collecting skin-resident microorganisms, which is used for collecting skin-resident microorganisms on the skin surface.

[0022] The invention comprises a substrate and an adhesive layer provided on at least a part of the surface of the substrate.

[0023] The adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive.

[0024] According to JIS Z 0237:2009, the adhesive force is 10.0N or more when measured in the following manner: the test piece is 12mm wide, the adherend is a bakelite, the pressing method is to use a 2kg roller to go back and forth once, the stretching speed is 300mm / min, the stretching angle is 90°,

[0025] The peeling force measured in accordance with JIS Z 0237:2009 is 0.25 N or more: the test piece is 12 mm wide, a silicone-treated chemical pulp paper is used as the release paper, the stretching speed is 300 mm / min, and the stretching angle is 180°.

[0026] (2) The adhesive tape for collecting skin-resident microorganisms according to (1) above, wherein the rubber-based adhesive contains natural rubber.

[0027] (3) The adhesive tape for collecting skin-resident microorganisms according to (1) or (2) above, which is used for collecting skin-resident microorganisms on the surface of the skin having a sebum content of 75% or more.

[0028] (4) A method for evaluating a subject's physical condition, the method comprising the following steps:

[0029] For the existence ratio of skin-resident microorganisms on the skin surface or a parameter based on the existence ratio for which a correlation benchmark with the physical condition has been established, a value of the skin microbial community collected from the skin surface of the subject is determined, and compared with the correlation benchmark,

[0030] The skin microbial community collected from the skin surface of the subject is obtained by collecting the skin resident microorganisms on the skin surface using the adhesive tape for collecting skin resident microorganisms described in any one of (1) to (3) above.

[0031] (5) The method according to (4) above, wherein the skin surface from which the skin microbial community is collected has a sebum content of 75% or more.

[0032] (6) A method for presenting information to a subject, the method comprising the following steps:

[0033] A step of evaluating the physical condition of the subject using the method described in (4) or (5) above; and

[0034] When it is evaluated that the body state is not in the target state, a step is prompted for registering information in a database as the following component, wherein the component is capable of guiding the value of the existence ratio or the parameter toward one side of the numerical range displayed by the existence ratio or the parameter based on the correlation benchmark when the body state is in the target state.

[0035] (7) The method according to (6) above, wherein the method further comprises the following steps:

[0036] A step of re-evaluating the physical condition of the subject after ingesting the ingredient using the method described in (4) or (5) above; and

[0037] The step of updating the information based on the degree of improvement of the re-evaluated physical condition.

[0038] (8) A method for screening a substance for improving or preventing a physical condition, the method comprising the following steps:

[0039] selecting the candidate substance based on the change caused by the candidate substance in the existence ratio of skin-resident microorganisms on the skin surface or the value of a parameter based on the existence ratio, for which a correlation benchmark with the body condition has been established,

[0040] The skin microbial community collected from the skin surface is obtained by collecting skin resident microorganisms from the skin surface of a subject using the adhesive tape for collecting skin resident microorganisms described in any one of (1) to (3).

[0041] (9) An adhesive tape for collecting skin metabolites, which is used for collecting skin metabolites on the skin surface.

[0042] The invention comprises a substrate and an adhesive layer provided on at least a part of the surface of the substrate.

[0043] The adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive.

[0044] According to JIS Z 0237:2009, the adhesive force is 10.0N or more when measured in the following manner: the test piece is 12mm wide, the adherend is a bakelite, the pressing method is to use a 2kg roller to go back and forth once, the stretching speed is 300mm / min, the stretching angle is 90°,

[0045] The peeling force measured in accordance with JIS Z 0237:2009 is 0.25 N or more: the test piece is 12 mm wide, a silicone-treated chemical pulp paper is used as the release paper, the stretching speed is 300 mm / min, and the stretching angle is 180°.

[0046] (10) The adhesive tape for collecting skin metabolites according to (9), wherein the rubber-based adhesive contains natural rubber.

[0047] (11) The adhesive tape for collecting skin metabolites according to (9) or (10), which is used for collecting skin metabolites on the skin surface having a sebum content of 75% or more.

[0048] (12) The adhesive tape for collecting skin metabolites according to any one of (9) to (11), wherein the skin metabolites are soluble proteins.

[0049] (13) A method for evaluating a subject's physical condition, comprising the following steps:

[0050] For the existence ratio of skin metabolites on the skin surface or a parameter based on the existence ratio for which a correlation benchmark with the physical state has been established, determining the value of the skin metabolites collected from the skin surface of the subject, and comparing it with the correlation benchmark,

[0051] The skin metabolites collected from the skin surface of the subject are obtained by collecting the skin metabolites from the skin surface using an adhesive tape for collecting skin metabolites.

[0052] (14) The method according to (13), wherein the amount of sebum on the skin surface from which the skin metabolites are collected is 75% or more.

[0053] (15) A method for presenting information to a subject, comprising the following steps:

[0054] A step of evaluating the physical condition of the subject using the method described in (13) or (14); and

[0055] When it is evaluated that the body state is not in the target state, a step is prompted for registering information in a database as the following component, wherein the component is capable of guiding the value of the existence ratio or the parameter toward one side of the numerical range displayed by the existence ratio or the parameter based on the correlation benchmark when the body state is in the target state.

[0056] (16) The method according to (15), wherein the method further comprises the following steps:

[0057] A step of re-evaluating the physical condition of the subject after ingesting the ingredient using the method described in (13) or (14); and

[0058] The step of updating the information based on the degree of improvement of the re-evaluated physical condition.

[0059] (17) A method for screening a substance for improving or preventing a physical condition, the method comprising the following steps:

[0060] selecting the candidate substance based on the change caused by the candidate substance in the existence ratio or the value of the parameter based on the existence ratio of the skin metabolites on the skin surface for which a correlation benchmark with the physical condition has been established,

[0061] The skin metabolites collected from the skin surface are obtained by collecting skin metabolites from the skin surface of a subject using the adhesive tape for collecting skin metabolites according to any one of (9) to (12).

[0062] Effects of the Invention

[0063] According to the present invention, an adhesive tape for collecting skin resident microorganisms that can increase the amount of skin resident microorganisms collected on the skin surface can be provided. In addition, an adhesive tape for collecting skin metabolites that can well collect skin metabolites on the skin surface can be provided. In addition, by using the adhesive tape for collecting skin resident microorganisms or the adhesive tape for collecting skin metabolites, a method for evaluating the physical condition of a subject with high accuracy, a method for presenting information to a subject, and a method for screening substances that improve or prevent physical conditions can be provided. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] Figure 1 This is a perspective view showing an example of the adhesive tape for collecting skin-resident microorganisms of the present invention.

[0065] Figure 2 This is a process diagram showing an example of a method for measuring a bacterial community using the adhesive tape for collecting skin-resident microorganisms of the present invention.

[0066] Figure 3 It is a schematic diagram which shows the measuring method of adhesive force.

[0067] Figure 4 It is a schematic diagram which shows the measuring method of peeling force.

[0068] Figure 5 It is a graph showing the measurement results of the total amount of DNA of subject A.

[0069] Figure 6 It is a graph showing the measurement results of the total amount of DNA of subject B.

[0070] Figure 7 It is a graph showing the measurement results of the total amount of DNA of subject C.

[0071] Figure 8 It is a graph showing the measurement results of the total amount of DNA of subject D.

[0072] Fig. 9 It is a graph showing the measurement results of the total amount of DNA of subject E. DETAILED DESCRIPTION

[0073] The present invention will be described in detail below. It should be noted that the present invention is not limited to the following contents, and various modifications can be made within the scope of the present invention.

[0074] <Adhesive tape for collecting skin-resident microorganisms>

[0075] The adhesive tape for collecting skin resident microorganisms of the present invention is used for collecting skin resident microorganisms on the skin surface, and comprises a substrate and an adhesive layer disposed on at least a portion of the surface of the substrate. Furthermore, the adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive. In addition, for the adhesive tape for collecting skin resident microorganisms, according to JIS Z 0237:2009, the adhesive force when measured in the following manner is 10.0N or more: the test piece is 12mm wide, the adherend is a bakelite board, the pressing method is to use a 2kg roller to go back and forth once, the stretching speed is 300mm / min, and the stretching angle is 90°; according to JIS Z 0237:2009, the peeling force when measured in the following manner is 0.25N or more: the test piece is 12mm wide, chemical pulp paper treated with organic silicon is used as the release paper, the stretching speed is 300mm / min, and the stretching angle is 180°.

[0076] use Figure 1 The adhesive tape for collecting skin-resident microorganisms of the present invention will be described in detail. Figure 1 This is a perspective view showing an example of the adhesive tape for collecting skin-resident microorganisms of the present invention.

[0077] like Figure 1As shown, the adhesive tape 1 for collecting skin-resident microorganisms of the present invention comprises a substrate 2 and an adhesive layer 3 provided on the surface of the substrate 2 . Figure 1 The figure shows a case where the entire surface of the substrate 2 has the adhesive layer 3, but the adhesive layer 3 only needs to be provided on at least a portion of the surface of the substrate 2. For example, the adhesive layer 3 may not be provided on a portion of the surface of the substrate 2 so that the substrate 2 is exposed.

[0078] The adhesive layer 3 contains an adhesive, and the adhesive contains a rubber-based adhesive.

[0079] The rubber-based adhesive contained in the adhesive layer 3 may be one or two or more selected from natural rubber and synthetic rubber, but preferably contains natural rubber.

[0080] In addition, for the adhesive tape for collecting skin resident microorganisms of the present invention, the adhesive force (hereinafter also referred to as "adhesive force") measured according to JIS Z 0237:2009 with a test piece of 12 mm width, an adherend of a bakelite, a 2 kg roller reciprocating once, a tensile speed of 300 mm / min, and a tensile angle of 90° is 10.0 N or more, preferably 11.0 N or more, more preferably 12.0 N or more, and further preferably 13.0 N or more. The upper limit of the adhesive force is not particularly limited, for example, 20.0 N or less, preferably 19.0 N or less, more preferably 18.0 N or less, and further preferably 17.0 N or less.

[0081] In addition, for the adhesive tape for collecting skin resident microorganisms of the present invention, according to JIS Z 0237: 2009, the peeling force (hereinafter also referred to as "peeling force") when measured with a test piece of 12 mm width, a silicone-treated chemical pulp paper as a release paper, a tensile speed of 300 mm / min, and a tensile angle of 180° is 0.25 N or more, preferably 0.26 N or more, more preferably 0.27 N or more, and further preferably 0.28 N or more. The upper limit of the peeling force is not particularly limited, for example, 0.50 N or less, preferably 0.45 N or less, more preferably 0.40 N or less, and further preferably 0.35 N or less.

[0082] Thus, by using an adhesive tape for collecting skin resident microorganisms having an adhesive layer 3 containing a rubber-based adhesive, an adhesive force of 10.0N or more, and a peeling force of 0.25N or more, as shown in the embodiments and comparative examples described later, the amount of skin resident microorganisms collected on the skin surface can be increased compared to conventional adhesive tapes. Therefore, when using such an adhesive tape for collecting skin resident microorganisms having an adhesive layer containing a rubber-based adhesive, an adhesive force of 10.0N or more, and a peeling force of 0.25N or more to collect skin resident microorganisms on the skin surface, it is not inferior to, for example, the wet collection of skin resident microorganisms on the skin surface using a swab (cotton swab) that can well collect skin resident microorganisms on the skin surface. In addition, since the collection is performed using the adhesive tape, the collection technique is easy.

[0083] The thickness of the adhesive tape for collecting skin resident microorganisms of the present invention is not particularly limited, and is, for example, 0.01 mm or more, 0.10 mm or more, or 0.20 mm or more. In addition, the upper limit of the thickness is not particularly limited, and is, for example, 0.50 mm or less, or 0.40 mm or less, or 0.30 mm or less.

[0084] The site for collecting skin microorganisms using the adhesive tape for collecting skin-resident microorganisms of the present invention is not particularly limited, and examples thereof include the face, scalp, armpits, and navel, among which the face is preferred, and the forehead is particularly preferred.

[0085] In addition, the adhesive tape for collecting skin resident microorganisms of the present invention is preferably used for collecting skin resident microorganisms on the skin surface with a sebum content of 75% or more. The adhesive tape for collecting skin resident microorganisms of the present invention can greatly increase the amount of skin resident microorganisms collected on the skin surface with a sebum content of 75% or more. Of course, it can also be used for collecting skin resident microorganisms on the skin surface with a sebum content of less than 75%. It should be noted that, since the amount of sebum on the skin surface of the subject may change due to the environment or physical condition, even when collecting skin resident microorganisms on the skin surface with a sebum content of 75% or more, it is possible to collect from a wide range of subjects.

[0086] The skin resident microorganisms collected by the adhesive tape for collecting skin resident microorganisms of the present invention are not particularly limited, and may be bacteria or fungi, among which bacteria are preferred. Specific examples of skin resident microorganisms include, for example, bacteria belonging to the phylum Actinobacteria, bacteria belonging to the phylum Proteobacteria, bacteria belonging to the phylum Firmicutes, and Propionibacterium acnes (P. acnes) (also referred to as Cutibacterium acnes in recent years).

[0087] It should be noted that after collecting skin-resident microorganisms on the skin surface using the adhesive tape for collecting skin-resident microorganisms of the present invention, when the adhesive tape for collecting skin-resident microorganisms is stored (for example, after collecting skin-resident microorganisms on the skin surface using the adhesive tape for collecting skin-resident microorganisms, the collected skin microbial community is analyzed after waiting for a period of time), the temperature for storing the adhesive tape for collecting skin-resident microorganisms in a state in which the skin-resident microorganisms have been collected is not particularly limited, and it can be stored at room temperature, or it can be refrigerated or frozen.

[0088] <Application of adhesive tape for collecting skin-resident microorganisms>

[0089] The adhesive tape for collecting skin resident microorganisms of the present invention can be used in a method for evaluating the physical condition of a subject. The method for evaluating the physical condition of a subject comprises the following steps: determining the value of the skin microbial community collected from the skin surface of the subject for the existence ratio of skin resident microorganisms on the skin surface or a parameter based on the existence ratio for which a correlation benchmark with the physical condition has been established, and comparing it with the correlation benchmark, wherein the skin microbial community collected from the skin surface of the subject is obtained by collecting the skin resident microorganisms on the skin surface using the adhesive tape for collecting skin resident microorganisms of the present invention.

[0090] Thus, various physical conditions can be accurately evaluated. In addition, certain physical conditions that were previously generalized can be subdivided by the existence ratio of skin resident microorganisms or the value of parameters, thereby, it can be expected that the physical condition of the subject can be improved with higher accuracy. In addition, by using the above-mentioned adhesive tape for collecting skin resident microorganisms, the amount of skin resident microorganisms collected on the skin surface can be increased, so that the physical condition can be evaluated with high accuracy.

[0091] In addition, the adhesive tape for collecting skin resident microorganisms of the present invention can be used in a method for presenting information to a subject. The method for presenting information to a subject is a method comprising the following steps: a step of evaluating the subject's physical state using the above-mentioned method for evaluating the subject's physical state; and, when the physical state is evaluated as not being in the target state, a step of presenting information registered in a database as the following component, wherein the component can guide the value of the existence ratio or parameter to one side of the numerical range displayed based on the correlation criterion when the existence ratio or parameter is in the target state.

[0092] Thus, it is possible to present information related to the improvement of physical condition to the subject in a personalized manner. It should be noted that the so-called information here refers to the name of the ingredient or the preparation containing the ingredient, the usage and dosage of the ingredient / preparation, the manner, degree, and side effects of the expected improvement of physical condition, etc.

[0093] The method of presenting information to a subject preferably further comprises the following steps: a step of re-evaluating the physical condition of the subject after ingesting the ingredient using the above-mentioned method for evaluating the physical condition of the subject; and a step of updating the information based on the degree of improvement of the re-evaluated physical condition.

[0094] As a result, it can be expected that the information in the database can be improved, and the accuracy of improving the physical condition can be further improved.

[0095] The timing of re-evaluation is not particularly limited, and may be, for example, when a time period has passed after use of the ingredient at which improvement in physical condition can be expected (which can be included in the information in the database).

[0096] In addition, the adhesive tape for collecting skin resident microorganisms of the present invention can be used in a method for screening substances that improve or prevent physical conditions. The method for screening substances that improve or prevent physical conditions has the following steps: selecting a candidate substance based on the change caused by the candidate substance to the existence ratio of skin resident microorganisms on the skin surface or the value of a parameter based on the existence ratio, for which a correlation benchmark with the physical condition has been established, or the value of the parameter, wherein the skin microbial community collected from the skin surface is obtained by collecting the skin resident microorganisms on the skin surface of the subject using the above-mentioned adhesive tape for collecting skin resident microorganisms.

[0097] Thus, it can be expected to screen substances that improve or prevent physical conditions with a high probability.

[0098] It should be noted that the screening in the present invention means that as long as the probability of existence of the substance that improves or prevents the physical condition in the candidate substance group after screening is higher than that in the candidate substance group before screening, it does not require that the candidate substance after screening can definitely improve or prevent the physical condition. Therefore, it is preferred to further implement a step of confirming that the candidate substance after screening has the effect of improving or preventing the physical condition.

[0099] The above methods are further described in detail.

[0100] Skin resident microorganisms may be bacteria or fungi, preferably bacteria. Their presence ratio may be related to any one of phylum, class, order, family, genus and species. For example, it may be the presence ratio of at least one bacterium selected from the group consisting of bacteria belonging to the phylum Actinomycetes, bacteria belonging to the phylum Proteobacteria and bacteria belonging to the phylum Firmicutes, among which the presence ratio of Propionibacterium acnes (P. acnes) may be cited.

[0101] The parameter is not particularly limited when it is based on the existence ratio of skin-resident microorganisms, and can be the difference, sum, product, ratio, exponent, logarithm or combination thereof of the existence ratios of multiple skin-resident microorganisms, wherein it can also be a ratio, difference or combination thereof. For example, the difference in the relative amount of bacterial species belonging to the Proteobacteria and the bacterial species belonging to the Firmicutes can be cited. Alternatively, the parameter can also be a diversity index of skin-resident microorganisms. This parameter can be related to the coordinate axis when each sample data of the population is plotted in the coordinate space using a dimensionality reduction technique (e.g., principal coordinate analysis, principal component analysis).

[0102] Physical state can be any state related to any position such as skin, hair, teeth, urine, bone, internal organs, blood, nerves, etc., as long as it is the physical state with correlation between the existence ratio of skin resident microorganisms on the skin surface or the parameters based on the existence ratio. As the state of skin, skin age, oily skin, acne skin, freckles, texture, yellowing, dryness, oiliness, spots, dullness, relaxation, wrinkles, tension state, pore state, yellowing of skin, etc. can be enumerated. As the state of hair, sparse hair, alopecia, etc. can be enumerated. As the state of teeth, periodontal disease and alveolar pyorrhea, etc. can be enumerated. As the state of bones, internal organs, blood, urine, nerves, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorders, diabetes, etc. can be enumerated. In addition, female hormones and menopause that can have an impact on beauty are also included in the physical state.

[0103] The correlation criterion is a criterion that can determine the goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the above-mentioned existence ratio or parameter value obtained from the skin microbial community.

[0104] According to the analysis results of the bacterial community obtained by the inventors of the present application, as shown in Patent Document 2, if the relative amount of bacteria belonging to the Actinobacteria, which are specific resident bacteria existing on the surface of facial skin, and the ratio of the amount of bacteria belonging to the Proteobacteria to the amount of bacteria belonging to the Firmicutes are investigated, it is possible to evaluate the susceptibility of various physical symptoms not limited to the face, such as acne, enlarged pores, and various facial skin conditions, periodontal disease, and thinning hair and hair loss on the head.

[0105] In addition, the relative amount of a specific bacterial species is correlated with the ease of causing other physical symptoms. For example, the occurrence of high uric acid levels (Rothia, Brevibacterium, Erythrobacteraceae, etc.), osteoporosis (Pseudomonas, Methylobacterium), decreased liver function (Campylobacter, etc.), yellow skin (Bacillus, Rothia dentocariosa, etc.), diabetes (bacteria belonging to the order Actinomycetales), etc., is correlated with the relative amount of the bacterial species in parentheses.

[0106] In addition, the skin aging index calculated based on the presence or absence of sagging, wrinkles, loss of tension, etc. on the facial skin showed a correlation with the presence of a group of bacteria that can be called "skin aging bacteria", including bacterial species belonging to the class Proteobacteria and Propionibacterium granulosum.

[0107] Many physical conditions are not only correlated with skin microbial communities, but also correlated with other matters (such as actual age). Therefore, the correlation benchmark is preferably a correlation benchmark that excludes the influence of at least one of the matters known to be related to the physical condition. In this way, it is possible to suppress the influence of the presence or degree of the above-mentioned other matters on the subject, and to perform a more accurate evaluation of the physical condition based on the existence ratio of the skin microbial community or the value of the parameter.

[0108] In addition, in the method of presenting information to the subject, the presence or absence and degree of the above-mentioned other matters about the subject can be considered to present more appropriate information. Therefore, it is preferred that in addition to the above-mentioned existence ratio or parameter, the presence or absence and degree of the above-mentioned other matters about the subject are further input.

[0109] In addition, in the method for screening a substance that improves or prevents a physical condition, a substance that can improve or prevent a physical condition can be easily obtained regardless of the above-mentioned other matters of the user.

[0110] In addition, the correlation criterion may be a correlation criterion established for a sub-population obtained by classifying the population according to at least one of the group consisting of age, gender, place of residence, nationality and race. Thus, it is possible to accurately evaluate the physical condition of the subject in consideration of the subject's age, gender, place of residence, nationality and race.

[0111] In addition, in the method of presenting information to the subject, the age, gender, place of residence, nationality and race of the subject may be considered to present more appropriate information. Therefore, it is preferred that, in addition to the above-mentioned existence ratio or parameter, at least one of the group consisting of age, gender, place of residence, nationality and race of the subject is further input.

[0112] In addition, in the method of screening a substance that improves or prevents a physical condition, it is easy to obtain a substance that can improve or prevent a physical condition that is suitable for the user's age, gender, place of residence, nationality, and race.

[0113] The site for collecting skin microbial communities is not particularly limited, and may be the face, scalp, armpits, navel, etc. Among them, the face, particularly the forehead, can be mentioned.

[0114] The site for collecting skin microbial communities is preferably a skin surface with a sebum content of 75% or more. This is because the above-mentioned adhesive tape for collecting skin resident microorganisms can greatly increase the amount of skin resident microorganisms collected from the skin surface with a sebum content of 75% or more. Of course, the sebum content of the site for collecting skin microbial communities can also be less than 75. It should be noted that the sebum content of the skin surface of the subject will change due to the environment or physical condition, so even if the site for collecting skin microbial communities is set to a skin surface with a sebum content of 75% or more, it can be applied to a large number of subjects.

[0115] It should be noted that the sampling site of the population sample for preparing the correlation reference is preferably consistent with the sampling site of the subject sample.

[0116] The value of the skin microbial community collected from the skin surface of the subject can be determined by analyzing the skin microbial community collected from the skin surface of the subject using the adhesive tape for collecting skin-resident microorganisms of the present invention.

[0117] The analysis of the bacterial community on the skin surface is preferably performed by analyzing the DNA present on the skin surface. Compared with the method of culturing bacteria collected from the skin surface with agar medium and estimating the bacterial community, the method of investigating the bacterial community using DNA can more accurately investigate the bacterial community. The reason is that it is reported that the proportion of microorganisms that can be cultured using agar is about 1%, and almost all microbial species are difficult to culture. In contrast, in the method of analyzing DNA, the analysis can be performed accurately while maintaining all the bacterial communities collected from the skin surface.

[0118] The analysis of the bacterial community is preferably performed using a method having the following steps:

[0119] A peeling step, in which the adhesive tape 1 for collecting skin resident microorganisms, which has brought the adhesive layer 3 into contact with the skin surface, is peeled off from the skin surface, so that the bacteria existing on the skin surface adhere to the adhesive layer;

[0120] an extraction step of bringing the adhesive layer 3 to which the bacteria are attached into contact with a liquid to extract the DNA contained in the bacteria; and

[0121] In the genome analysis step, the extracted DNA is subjected to genome analysis.

[0122] In the analysis method of this bacterial community, the skin resident microorganism collection adhesive tape 1 that has made adhesive layer 3 contact with skin surface is peeled off from the skin surface, so that the state that the bacteria present in the skin surface is attached to adhesive layer 3 is recovered (peeling step).Thus, the bacteria present in the skin surface, the skin surface contacted with adhesive layer 3 is attached to the adhesive layer 3 side of the skin resident microorganism collection adhesive tape 1 with its original distribution state and collected.Therefore, it will not cause the data of bacterial community to produce deviation due to the difference of wiping force and wiping range like the method of wiping the skin surface with cotton swab etc. In addition, the bacterial community of skin surface can be faithfully reproduced.And then, the adhesive layer 3 attached with bacteria is further contacted with liquid, and the DNA contained in the bacteria is extracted (extraction step).Therefore, compared with the method that the material attached to the surface of adhesive layer is distinguished and provided for the sample using a magnifying glass etc., the state of DNA solution that can measure bacterial community can be formed extremely quickly from the adhesive component attached with bacteria.Therefore, the change that bacterial community occurs in the process time for sample collection can be minimized. In addition, by analyzing the extracted DNA using a next-generation sequencer (NGS) or the like, the bacterial community present on the skin surface can be easily and evenly measured.

[0123] In addition, the extraction method and analysis method of DNA are not particularly limited.

[0124] <Adhesive tape for collecting skin metabolites>

[0125] The adhesive tape for collecting skin metabolites of the present invention can be used for collecting skin metabolites on the skin surface, and comprises a substrate and an adhesive layer disposed on at least a portion of the surface of the substrate. Furthermore, the adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive. In addition, for the adhesive tape for collecting skin metabolites, according to JIS Z 0237:2009, the adhesive force when measured in the following manner is 10.0N or more: the test piece is 12mm wide, the adherend is a bakelite board, the pressing method is to use a 2kg roller to go back and forth once, the stretching speed is 300mm / min, and the stretching angle is 90°; according to JIS Z 0237:2009, the peeling force when measured in the following manner is 0.25N or more: the test piece is 12mm wide, chemical pulp paper treated with organic silicon is used as the release paper, the stretching speed is 300mm / min, and the stretching angle is 180°.

[0126] The adhesive tape for collecting skin metabolites of the present invention has the same configuration as the adhesive tape 1 for collecting skin resident microorganisms.

[0127] The adhesive tape for collecting skin metabolites of the present invention comprises a substrate and an adhesive layer provided on the surface of the substrate. The adhesive layer may be provided on at least a portion of the surface of the substrate. For example, the adhesive layer may be provided on the entire surface of one surface of the substrate. In addition, the adhesive layer may not be provided on a portion of the surface of the substrate so that the substrate is exposed.

[0128] The adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive.

[0129] The rubber-based adhesive contained in the adhesive layer may be one or two or more selected from natural rubber and synthetic rubber, but preferably contains natural rubber.

[0130] Furthermore, for the adhesive tape for collecting skin metabolites of the present invention, the adhesive force (hereinafter also referred to as "adhesive force") measured according to JIS Z0237:2009 with a test piece of 12 mm width, an adherend of a bakelite, a 2 kg roller reciprocating once, a tensile speed of 300 mm / min, and a tensile angle of 90° is 10.0 N or more, preferably 11.0 N or more, more preferably 12.0 N or more, and further preferably 13.0 N or more. The upper limit of the adhesive force is not particularly limited, and is, for example, 20.0 N or less, preferably 19.0 N or less, more preferably 18.0 N or less, and further preferably 17.0 N or less.

[0131] In addition, for the adhesive tape for collecting skin metabolites of the present invention, the peeling force (hereinafter also referred to as "peeling force") measured according to JIS Z0237:2009 with a test piece having a width of 12 mm, using chemical pulp paper treated with organic silicon as release paper, a tensile speed of 300 mm / min, and a tensile angle of 180° is 0.25 N or more, preferably 0.26 N or more, more preferably 0.27 N or more, and further preferably 0.28 N or more. The upper limit of the peeling force is not particularly limited, and is, for example, 0.50 N or less, preferably 0.45 N or less, more preferably 0.40 N or less, and further preferably 0.35 N or less.

[0132] In this way, by using an adhesive tape for collecting skin metabolites having an adhesive layer containing a rubber-based adhesive, an adhesive force of more than 10.0N and a peeling force of more than 0.25N, it is possible to well collect metabolites on the skin surface, specifically, on the skin or in the skin on the skin surface. Moreover, if the adhesive tape for collecting skin metabolites is used, the amount of metabolites collected on the skin surface is much greater than when using the previous tape. Therefore, the use of such an adhesive tape for collecting skin metabolites having an adhesive layer containing a rubber-based adhesive, an adhesive force of more than 10.0N and a peeling force of more than 0.25N to collect skin metabolites on the skin surface is not inferior to the use of a swab (cotton swab) that can well collect skin metabolites on the skin surface by wet collection. In addition, since the tape is used for collection, the collection technique is easy.

[0133] The thickness of the adhesive tape for collecting skin metabolites of the present invention is not particularly limited, and may be, for example, 0.01 mm or more, 0.10 mm or more, or 0.20 mm or more. In addition, the upper limit of the thickness is not particularly limited, and may be, for example, 0.50 mm or less, or 0.40 mm or less, or 0.30 mm or less.

[0134] The site for collecting skin metabolites using the adhesive tape for collecting skin metabolites of the present invention is not particularly limited, and examples thereof include face, scalp, armpits, and navel, among which face is preferred, and forehead is particularly preferred.

[0135] In addition, the adhesive tape for collecting skin metabolites of the present invention is preferably used for collecting skin metabolites on the skin surface with a sebum content of 75% or more. The adhesive tape for collecting skin metabolites of the present invention can greatly increase the amount of skin metabolites collected on the skin surface with a sebum content of 75% or more. Of course, it can also be used to collect skin metabolites on the skin surface with a sebum content of less than 75%. It should be noted that since the amount of sebum on the skin surface of the subject may change due to the environment or physical condition, even when collecting skin metabolites on the skin surface with a sebum content of 75% or more, it can be collected from a wide range of subjects.

[0136] The skin metabolites collected by the adhesive tape for collecting skin metabolites and skin-resident microorganisms of the present invention are not particularly limited, and may be, for example, soluble proteins.

[0137] In the embodiment of the present invention, the total amount of soluble proteins can be measured, for example, by biochemical quantitative analysis (BCA method, etc.). In addition, the identification or quantification of each soluble protein can be performed, for example, by a biochemical method using antibodies (Western blotting method, ELISA method, etc.) or a mass spectrometer.

[0138] It should be noted that after collecting skin metabolites on the skin surface using the adhesive tape for collecting skin metabolites of the present invention, when the adhesive tape for collecting skin metabolites is stored (for example, after collecting skin metabolites on the skin surface using the adhesive tape for collecting skin metabolites, the collected skin metabolites are analyzed after waiting for a period of time), the temperature for storing the adhesive tape for collecting skin metabolites in a state in which the skin metabolites have been collected is not particularly limited, and it can be stored at room temperature, or it can be refrigerated or frozen.

[0139] <Application of adhesive tape for collecting skin metabolites>

[0140] The adhesive tape for collecting skin metabolites of the present invention can be used in a method for evaluating the physical condition of a subject. The method for evaluating the physical condition of a subject comprises the following steps: determining the value of the skin metabolites collected from the skin surface of the subject for the existence ratio of the skin metabolites on the skin surface for which a correlation benchmark with the physical condition has been established or a parameter based on the existence ratio, and comparing it with the correlation benchmark, wherein the skin metabolites collected from the skin surface of the subject are obtained by collecting the skin metabolites on the skin surface using the adhesive tape for collecting skin metabolites of the present invention.

[0141] Thus, various physical conditions can be accurately evaluated. In addition, certain physical conditions that were previously generalized can be subdivided by the existence ratio of skin metabolites or the value of parameters, thereby, it can be expected that the physical condition of the subject can be improved with higher accuracy. In addition, by using the above-mentioned adhesive tape for collecting skin metabolites, the amount of skin metabolites collected on the skin surface is good, so the physical condition can be evaluated with high accuracy.

[0142] In addition, the adhesive tape for collecting skin metabolites of the present invention can be used in a method for presenting information to a subject. The method for presenting information to a subject is a method comprising the following steps: a step of evaluating the subject's physical state using the above-mentioned method for evaluating the subject's physical state; and, when the physical state is evaluated as not being in the target state, a step of presenting information registered in a database as the following component, wherein the component can guide the value of the existence ratio or parameter to one side of the numerical range displayed based on the correlation criterion when the existence ratio or parameter is in the target state.

[0143] Thus, it is possible to present information related to the improvement of physical condition to the subject in a personalized manner. It should be noted that the so-called information here refers to the name of the ingredient or the preparation containing the ingredient, the usage and dosage of the ingredient / preparation, the manner, degree, and side effects of the expected improvement of physical condition, etc.

[0144] The method of prompting a subject with information preferably further comprises the following steps:

[0145] A step of re-evaluating the physical condition of the subject after ingesting the ingredient using the above-mentioned method for evaluating the physical condition of the subject; and a step of updating information based on the degree of improvement of the re-evaluated physical condition.

[0146] As a result, it can be expected that the information in the database can be improved, and the accuracy of improving the physical condition can be further improved.

[0147] The timing of re-evaluation is not particularly limited, and may be, for example, when a time period has passed after use of the ingredient at which improvement in physical condition can be expected (which can be included in the information in the database).

[0148] In addition, the adhesive tape for collecting skin metabolites of the present invention can be used in a method for screening substances that improve or prevent physical conditions. The method for screening substances that improve or prevent physical conditions has the following steps: selecting a candidate substance based on the change caused by the candidate substance to the existence ratio of skin metabolites on the skin surface or the value of a parameter based on the existence ratio, for which a correlation benchmark with the physical condition has been established, and the skin metabolites collected from the skin surface are obtained by collecting the skin metabolites on the skin surface of the subject using the above-mentioned adhesive tape for collecting skin metabolites.

[0149] Thus, it can be expected to screen substances that improve or prevent physical conditions with a high probability.

[0150] It should be noted that the screening in the present invention means that as long as the probability of existence of the substance that improves or prevents the physical condition in the candidate substance group after screening is higher than that in the candidate substance group before screening, it does not require that the candidate substance after screening can definitely improve or prevent the physical condition. Therefore, it is preferred to further implement a step of confirming that the candidate substance after screening has the effect of improving or preventing the physical condition.

[0151] The above methods are further described in detail.

[0152] The parameter is not particularly limited when it is based on the existence ratio of skin metabolites, and can be the difference, sum, product, ratio, index, logarithm or combination of the existence ratios of multiple skin metabolites, wherein it can also be a ratio, difference or combination thereof. Alternatively, the parameter can also be a diversity index of skin metabolites. Such a parameter can be related to the coordinate axis when the sample data of the population is plotted in the coordinate space using a dimensionality reduction technique (e.g., principal coordinate analysis, principal component analysis).

[0153] As long as the physical state is a physical state with a correlation between the existence ratio of skin metabolites on the skin surface or the parameters based on the existence ratio, it can be a state related to any position such as skin, hair, teeth, urine, bone, internal organs, blood, nerves, etc. As the state of the skin, skin age, oily skin, acne skin, freckles, texture, yellowing, dryness, oiliness, spots, dullness, relaxation, wrinkles, tension state, pore state, yellowing of the skin, etc. can be cited. As the state of hair, sparse hair, hair loss, etc. can be cited. As the state of teeth, periodontal disease and alveolar pyorrhea, etc. can be cited. As the state of bones, internal organs, blood, urine, nerves, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorders, diabetes, etc. can be cited. In addition, female hormones and menopause that can affect beauty are also included in the physical state.

[0154] The correlation criterion is a criterion that can determine the goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the above-mentioned existence ratio or parameter value obtained from the skin metabolites.

[0155] Many physical conditions are not only correlated with skin metabolites, but also correlated with other matters (such as actual age). Therefore, the correlation criterion is preferably a correlation criterion that excludes the influence of at least one of the matters known to be related to the physical condition. In this way, the influence of the presence or absence and degree of the above-mentioned other matters on the subject can be suppressed, and a more accurate evaluation of the physical condition can be performed based on the existence ratio of skin metabolites or the value of the parameter.

[0156] In addition, in the method of presenting information to the subject, the presence or absence and degree of the above-mentioned other matters about the subject can be considered to present more appropriate information. Therefore, it is preferred that in addition to the above-mentioned existence ratio or parameter, the presence or absence and degree of the above-mentioned other matters about the subject are further input.

[0157] In addition, in the method for screening a substance that improves or prevents a physical condition, a substance that can improve or prevent a physical condition can be easily obtained regardless of the above-mentioned other matters of the user.

[0158] In addition, the correlation criterion may be a correlation criterion established for a sub-population obtained by classifying the population according to at least one of the group consisting of age, gender, place of residence, nationality and race. Thus, it is possible to accurately evaluate the physical condition of the subject in consideration of the subject's age, gender, place of residence, nationality and race.

[0159] In addition, in the method of presenting information to the subject, the age, gender, place of residence, nationality and race of the subject may be considered to present more appropriate information. Therefore, it is preferred that, in addition to the above-mentioned existence ratio or parameter, at least one of the group consisting of age, gender, place of residence, nationality and race of the subject is further input.

[0160] In addition, in the method of screening a substance that improves or prevents a physical condition, it is easy to obtain a substance that can improve or prevent a physical condition that is suitable for the user's age, gender, place of residence, nationality, and race.

[0161] The site for collecting skin metabolites is not particularly limited, and may be the face, scalp, armpits, navel, etc. Among them, the face, particularly the forehead, can be mentioned.

[0162] The site for collecting skin metabolites is preferably a skin surface with a sebum content of 75% or more. This is because the above-mentioned adhesive tape for collecting skin metabolites can greatly increase the amount of skin metabolites collected from the skin surface with a sebum content of 75% or more. Of course, the sebum content of the site for collecting skin metabolites may also be less than 75%. It should be noted that the sebum content of the skin surface of the subject may change due to the environment or physical condition, so even if the site for collecting skin metabolites is set to a skin surface with a sebum content of 75% or more, it can be applied to a large number of subjects.

[0163] It should be noted that the sampling site of the population sample for preparing the correlation reference is preferably consistent with the sampling site of the subject sample.

[0164] The determination of the value of the skin metabolites collected from the skin surface of the subject is preferably carried out as follows: from the amount of skin metabolites collected from the skin surface of the subject using the above-mentioned adhesive tape for collecting skin metabolites of the present invention, the amount of skin metabolites derived from bacteria collected at the same time is subtracted, and the resulting value is calculated. It should be noted that the amount of skin metabolites derived from bacteria collected is very small compared to the amount of skin metabolites collected, so the value of the collected skin metabolites can be directly used for the evaluation of physical condition. If described in detail, the soluble proteins collected with the adhesive tape for collecting skin metabolites are considered to be almost all proteins derived from human skin cells. The reason is that, based on the amount of bacteria attached to the adhesive tape for collecting skin metabolites (obtained by qPCR measurement of 16S rRNA), the amount of total protein derived from bacteria per piece of adhesive tape for collecting skin metabolites is estimated to be less than 0.1 μg. It should be noted that the following known values ​​are used in this calculation, the number of 16S rRNA genes contained in 1 bacterium is about 4, and the total amount of protein is about 0.1 pg.

[0165] Specifically, first, the adhesive tape for collecting skin metabolites that has made the adhesive layer contact with the skin surface is peeled off from the skin surface so that the skin metabolites present on the skin surface are recovered in a state of being attached to the adhesive layer (peeling step). Thus, the skin metabolites on the skin surface that are in contact with the adhesive layer among the skin metabolites on the skin surface are attached to the adhesive layer side of the adhesive tape for collecting skin metabolites in their original distribution state and collected. Therefore, unlike the method of wiping the skin surface with a cotton swab, the data of the skin metabolites will not be biased due to the difference in wiping force and wiping range. In addition, the skin metabolites on the skin surface can be faithfully reproduced.

[0166] Furthermore, the adhesive layer to which the skin metabolites are attached is further brought into contact with a liquid (e.g., PBS: phosphate buffered saline), and ultrasonic treatment or the like is performed as needed to extract the skin metabolites (extraction step). Therefore, compared with a method in which a substance attached to the surface of the adhesive layer is separated and provided to a sample using a magnifying glass or the like, a state of a solution capable of measuring skin metabolites can be formed extremely quickly from an adhesive component to which skin metabolites are attached. Therefore, changes in skin metabolites during the process time for sample collection can be minimized. In addition, by analyzing the extracted skin metabolites using a commercially available soluble protein assay kit or the like, the amount of soluble proteins, etc. present on the skin surface can be simply and uniformly measured.

[0167] It should be noted that the extraction method and analysis method of skin metabolites are not particularly limited.

[0168] Example

[0169] Hereinafter, the present invention will be described in further detail based on Examples, but the present invention is not limited thereto.

[0170] <<Adhesive tape for collecting skin-resident microorganisms>>

[0171] <Example 1>

[0172] (Collection of bacterial communities and determination of total DNA)

[0173] Bacterial communities were collected from subjects A to E respectively.

[0174] The collection of bacterial communities and the determination of the total amount of DNA were carried out according to Figure 2 Proceed as shown in the flowchart.

[0175] Contact step S1 and peeling step S2

[0176] Prepare Figure 1 The adhesive tape 1 for collecting resident microorganisms of the skin is shown. The adhesive tape 1 for collecting resident microorganisms of the skin is a structure in which an adhesive layer 3 formed by an adhesive is provided on a thin sheet-shaped tape substrate 2 as a base. Therefore, it can be operated in the same way as a common bandage or dressing tape, and the contact step S1 can be simply performed by sticking it on human skin. The adhesive tape 1 for collecting resident microorganisms of the skin is pasted on a release paper not shown in the figure, and is sent to the subject together with latex gloves in a state of being sealed in a sealable aluminum bag containing a desiccant. The subject puts on latex gloves just after getting up and before washing his face, unseals the aluminum bag and takes out the release paper pasted with the adhesive tape 1 for collecting resident microorganisms of the skin, peels off the adhesive tape 1 for collecting resident microorganisms of the skin and sticks it on the forehead of the subject. Then, after keeping the adhesive state for 5 minutes, the adhesive tape 1 for collecting resident microorganisms of the skin is peeled off, and it is pasted back on the release paper, and it is put into the aluminum bag together with the desiccant and sealed. Thereafter, the samples were frozen and stored until the DNA analysis test was performed. Table 1 shows the types of the base material and adhesive of the adhesive tape 1 for collecting skin-resident microorganisms.

[0177] Extraction step S3

[0178] According to the method of Morita et al. (Microbes Environ. Vol22, No.3, 214-222, 2007), DNA was directly extracted from the adhesive tape 1 for collecting resident microorganisms on the skin to prepare a DNA solution. The total amount of DNA was then measured from the DNA solution. Specifically, 1 / 20 of the amount of the DNA solution was used as a sample sample, and a KAPA SYBR (registered trademark) real-time PCR reagent containing DNA polymerase (manufactured by Kapa Biosystems, product name: KAPA SYBR (registered trademark) Fast qPCR Kit) was used to perform a copy number analysis of the 16S rRNA gene using a real-time PCR device (manufactured by Applied Biosystems, product name: StepOnePlusTM). In addition, a DNA fragment with the same amplification efficiency as the target molecule was used as an internal standard to prepare a calibration curve.

[0179] (Determination of Sebum Amount)

[0180] Before washing the face on the day of collecting the bacterial community, the amount of sebum on the forehead of subjects A to E was measured. First, adapt to room temperature at 25°C ± 1°C for 20 minutes. Use a sebum meter (manufactured by Courage + Khazaka, product name: Sebmeter SM815) to measure the amount of sebum secreted on the skin surface after adaptation. Specifically, press the sebum meter on the top of the right cheekbone for 30 seconds, measure the amount of sebum in the adjacent parts 3 times, and take the average of the 3 measured values ​​as the amount of sebum. In addition, when there are moles or inflammations at the measurement site, avoid the site for measurement. It should be noted that the amount of sebum is classified according to skin quality. The amount of sebum on the cheeks is 70 or less for "dry, less sebum", 70-180 for "normal", and 180 or more for "oily".

[0181] As a result, the sebum amount of subject A was 146.3, the sebum amount of subject B was 137.0, the sebum amount of subject C was 104.7, the sebum amount of subject D was 82.7, and the sebum amount of subject E was 76.3.

[0182] (Measurement of Adhesive Strength of Adhesive Tape 1 for Collecting Skin-Resident Microorganisms)

[0183] A test piece 10 having a width of 12 mm was prepared from the adhesive tape 1 for collecting resident microorganisms on the skin. The adhesive force was measured using the test piece 10 in accordance with JIS Z 0237:2009. Specifically, the test piece 10 made of the adhesive tape 1 for collecting resident microorganisms on the skin, which is formed by the base material 1 and the adhesive layer 3, was laminated on a bakelite board as the adherend 11 by reciprocating once with a 2 kg roller in such a manner that the adhesive layer 3 was located on one side of the adherend 11. And, as Figure 3As shown, the adhesive strength was measured at a tensile speed of 300 mm / min and a tensile angle of 90° using a tensile tester in accordance with JIS B7721:2009.

[0184] (Measurement of the peeling force of the adhesive tape 1 for collecting skin-resident microorganisms)

[0185] A test piece 20 with a width of 12 mm was prepared from the adhesive tape 1 for collecting skin resident microorganisms. The test piece 20 was used to measure the peeling force in accordance with JIS Z 0237:2009. Specifically, the test piece 20 made of the adhesive tape 1 for collecting skin resident microorganisms formed of the substrate 1 and the adhesive layer 3 was attached to the chemical pulp paper treated with silicone as the release paper 21 in such a manner that the adhesive layer 3 was located on the side of the surface treated with silicone of the release paper 21. And, as Figure 4 As shown, the film was placed on the auxiliary plate 22, and the peeling force was measured at a tensile speed of 300 mm / min and a tensile angle of 180° using a tensile tester in accordance with JIS B7721:2009.

[0186] <Comparative Examples 1 to 6>

[0187] The same operation as in Example 1 was performed except that the adhesive tape 1 for collecting skin resident microorganisms was changed to the one described in Table 1. It should be noted that in Comparative Example 5, the adhesive tape for collecting skin resident microorganisms of Comparative Example 4 was attached to both surfaces of the adhesive tape for collecting skin resident microorganisms of Example 1.

[0188] Table 1 shows the measurement results of the adhesive force and peeling force of the pressure-sensitive adhesive tape 1 for collecting skin-resident microorganisms and the thickness of the pressure-sensitive adhesive tape 1 for collecting skin-resident microorganisms.

[0189] In addition, regarding the total amount of DNA, the measurement results of subject A are shown in Figure 5 , the measurement results of subject B are shown in Figure 6 The measurement results of subject C are shown in Figure 7 The measurement results of subject D are shown in Figure 8 , the measurement results of subject E are shown in Fig. 9 .

[0190] The results are shown in Table 1 and Figures 5 to 9 As shown, Example 1 using an adhesive tape for collecting skin-resident microorganisms having an adhesive layer containing a rubber-based adhesive and having an adhesive force of 10 N / 12 mm or more and a peeling force of 0.25 N / mm or more has a much higher total DNA content than Comparative Examples 1 to 6.

[0191] [Table 1]

[0192]

[0193] <<Adhesive tape for collecting skin metabolites>>

[0194] (Collection of soluble protein and determination of the amount of soluble protein)

[0195] Soluble proteins are collected from subjects A, C to E respectively in the following steps S1 and S2, and the soluble proteins are extracted in the following step S3 to measure the amount of soluble proteins.

[0196] Contact step S1 and peeling step S2

[0197] The adhesive tape 1 for collecting resident microorganisms of the skin used in the above-mentioned embodiment 1 is used as an adhesive tape for collecting skin metabolites. As described above, the adhesive tape for collecting skin metabolites (adhesive tape 1 for collecting resident microorganisms of the skin) is a structure in which an adhesive layer formed by an adhesive is provided on a thin sheet-like tape substrate as a base. Therefore, it can be operated in the same way as a common bandage and dressing tape, and the contact step S1 is simply performed by sticking it on human skin. The adhesive tape for collecting skin metabolites is pasted on a release paper and sent to the subject together with latex gloves in a state where it is sealed in a sealable aluminum bag containing a desiccant. The subject puts on latex gloves just after getting up and before washing his face, unseals the aluminum bag and takes out the release paper pasted with the adhesive tape for collecting skin metabolites, peels off the adhesive tape for collecting skin metabolites and sticks it on the forehead of the subject. Then, after keeping the adhesive state for 5 minutes, the adhesive tape for collecting skin metabolites is peeled off, re-sticked on the release paper, and it is put into an aluminum bag together with a desiccant and sealed. Thereafter, the cells were frozen and stored until the soluble protein content was measured.

[0198] Extraction step S3

[0199] The skin metabolite collection adhesive tape obtained in the contact step S1 and the peeling step S2 and the skin metabolite collection adhesive tape without skin metabolites attached as a control were placed in a container added with 2 mL of PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation), and the container was subjected to ultrasonic disruption for 15 minutes. The ultrasonic disruption was performed using an ultrasonic disruptor (Bioruptor (registered trademark) PiCo, manufactured by diagenode), with 15 seconds of start and 15 seconds of stop as one cycle, and 30 cycles were performed.

[0200] Calculation of the amount of soluble protein from human skin cells

[0201] The total amount of soluble protein was measured using a 562 nm spectrophotometer using the ultrasonic disruption solution obtained in the extraction step S3, a measurement kit: BCA method microprotein concentration measurement kit (manufactured by Thermo Fisher Scientific), and a measurement device: Nanodrop ND-1000 (manufactured by Thermo Fisher Scientific).

[0202] In addition, the amount of soluble protein derived from bacteria collected simultaneously with the skin metabolite collection adhesive tape was calculated by the number of 16S rRNA gene copies ÷ the number of 16S rRNAs retained per unit of bacteria (3.5) × the amount of protein retained per unit of bacteria (0.1 pg). As a result, the amount of soluble protein derived from bacteria per tape in the examples and comparative examples was a very small amount of less than 0.04 μg.

[0203] From the total amount of soluble protein extracted by the adhesive tape for collecting skin metabolites obtained by the above-mentioned contact step S1 and stripping step S2, the amount of protein extracted by the adhesive tape for collecting skin metabolites without attached skin metabolites and the amount of soluble protein estimated to be derived from bacteria are subtracted, and the obtained value is taken as the amount of soluble protein derived from human skin cells.

[0204] (Determination of Sebum Amount)

[0205] Before washing the face on the day of collecting skin metabolites, the amount of sebum on the forehead of subjects A, C to E was measured respectively. First, adapt to room temperature of 25°C ± 1°C for 20 minutes. Use a sebum meter (manufactured by Courage + Khazaka, product name: Sebmeter SM815) to measure the amount of sebum secreted on the skin surface after adaptation. Specifically, press the sebum meter on the top of the right cheekbone for 30 seconds, measure the amount of sebum in adjacent positions 3 times, and take the average of the 3 measured values ​​as the amount of sebum. In addition, when there are moles or inflammations at the measurement site, avoid the site for measurement. It should be noted that the amount of sebum is classified according to skin quality. The amount of sebum on the cheeks is 70 or less for "dry, less sebum", 70-180 for "normal", and 180 or more for "oily".

[0206] As a result, the sebum amount of subject A was 146.3, the sebum amount of subject C was 104.7, the sebum amount of subject D was 61.3, and the sebum amount of subject E was 74.0.

[0207] The same operation as above was performed except that the adhesive tape for collecting skin metabolites was changed to the adhesive tapes of Comparative Examples 1, 2 and 6 shown in Table 1 instead of the adhesive tape for collecting skin resident microorganisms used in Example 1.

[0208] The total amount of soluble proteins extracted and the amount of soluble proteins believed to be derived from human skin cells are shown in Table 2 for subject A, in Table 3 for subject C, in Table 4 for subject D, and in Table 5 for subject E.

[0209] [Table 2]

[0210]

[0211] [Table 3]

[0212]

[0213] [Table 4]

[0214]

[0215] [Table 5]

[0216]

[0217] The results are shown in Table 1 and Figures 5 to 9 As shown, Example 1 using an adhesive tape for collecting skin-resident microorganisms having an adhesive layer containing a rubber-based adhesive and having an adhesive force of 10 N / 12 mm or more and a peeling force of 0.25 N / mm or more has a much higher total DNA content than Comparative Examples 1 to 6.

[0218] In addition, as shown in Tables 2 to 5, Example 1, which uses an adhesive tape for collecting skin metabolites having an adhesive layer containing a rubber-based adhesive and an adhesive force of 10 N / 12 mm or more and a peeling force of 0.25 N / mm or more, has a much higher amount of soluble protein than Comparative Examples 1, 2, and 6.

[0219] Reference numerals

[0220] 1 Adhesive tape for collecting skin resident microorganisms

[0221] 2. Substrate

[0222] 3 Adhesive layer

Claims

1. An adhesive tape for collecting skin resident microorganisms on the skin surface or collecting skin metabolites on the skin surface, The invention comprises a substrate and an adhesive layer provided on at least a part of the surface of the substrate. The adhesive layer contains an adhesive, and the adhesive contains a rubber-based adhesive. According to JIS Z 0237:2009, the adhesive force is 10.0N or more when measured in the following manner: the test piece is 12mm wide, the adherend is a bakelite, the pressing method is to use a 2kg roller to go back and forth once, the stretching speed is 300mm / min, the stretching angle is 90°, The peeling force measured in accordance with JIS Z 0237:2009 is 0.25 N or more: the test piece is 12 mm wide, a silicone-treated chemical pulp paper is used as the release paper, the stretching speed is 300 mm / min, and the stretching angle is 180°.

2. The adhesive tape according to claim 1, wherein The adhesive tape after collection contains skin-resident microorganisms or contains skin metabolites.

3. The adhesive tape according to claim 1, wherein The skin metabolites are soluble proteins.

4. Use of a skin microbial community collected from the skin surface of a subject in the preparation of a product for evaluating the physical condition of the subject, wherein the value of the skin microbial community collected from the skin surface of the subject is determined for the existence ratio of skin-resident microorganisms on the skin surface for which a correlation benchmark with the physical condition has been established or a parameter based on the existence ratio, and compared with the correlation benchmark, The skin microbial community collected from the skin surface of the subject is obtained by collecting the skin resident microorganisms on the skin surface using the adhesive tape according to claim 1 or 2, The physical condition is: The skin condition of at least one of acne skin, skin age, oily skin, skin freckles, skin texture, yellowing, dryness, oiliness, spots, dullness, sagging, wrinkles, tension, and pores, or At least one of thinning hair, hair loss, periodontal disease, alveolar pyorrhea, menopause, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorder and diabetes, The correlation criterion is a criterion that can determine the degree of goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the value of the presence ratio or parameter determined from the skin microbial community.

5. Use of a skin microbial community collected from the skin surface of a subject in the preparation of a product for providing information to the subject, wherein: In the process of prompting the subject with information: evaluating the physical condition of the subject by the evaluation according to claim 4; and When it is evaluated that the physical state is not in the target state, information registered in the database as the following component is prompted, and the component is capable of guiding the value of the existence ratio or the parameter to one side of the numerical range displayed by the existence ratio or the parameter based on the correlation benchmark when the physical state is in the target state.

6. The use according to claim 5, wherein: Re-evaluating the physical condition of the subject after ingesting the ingredient using the evaluation according to claim 4; and The information is updated based on the degree of improvement of the re-evaluated physical condition.

7. Use of a skin microbial community collected from the skin surface of a subject in the preparation of a product for screening a substance for improving or preventing a physical condition, wherein: selecting the candidate substance based on the change caused by the candidate substance in the existence ratio of skin-resident microorganisms on the skin surface or the value of a parameter based on the existence ratio, for which a correlation benchmark with the physical condition has been established, The skin microbial community collected from the skin surface of the subject is obtained by collecting the skin resident microorganisms on the skin surface of the subject using the adhesive tape according to claim 1 or 2, The physical condition is: The skin condition of at least one of acne skin, skin age, oily skin, skin freckles, skin texture, yellowing, dryness, oiliness, spots, dullness, sagging, wrinkles, tension, and pores, or At least one of thinning hair, hair loss, periodontal disease, alveolar pyorrhea, menopause, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorder and diabetes, The correlation criterion is a criterion that can determine the degree of goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the value of the presence ratio or parameter determined from the skin microbial community.

8. Use of skin metabolites collected from the skin surface of a subject in preparing a product for evaluating the physical condition of the subject, wherein the value of the skin metabolites collected from the skin surface of the subject is determined for an existence ratio of the skin metabolites on the skin surface for which a correlation benchmark with the physical condition has been established or a parameter based on the existence ratio, and compared with the correlation benchmark, The skin metabolites collected from the skin surface of the subject are obtained by collecting the skin metabolites from the skin surface using the adhesive tape according to any one of claims 1 to 3, The physical condition is: The skin condition of at least one of acne skin, skin age, oily skin, skin freckles, skin texture, yellowing, dryness, oiliness, spots, dullness, sagging, wrinkles, tension, and pores, or At least one of thinning hair, hair loss, periodontal disease, alveolar pyorrhea, menopause, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorder and diabetes, The correlation criterion is a criterion capable of determining the degree of goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the value of the abundance ratio or parameter obtained from the skin metabolites.

9. Use of skin metabolites collected from the skin surface of a subject in the preparation of a product for providing information to the subject, wherein: In the process of prompting the subject with information: evaluating the physical condition of the subject by the evaluation according to claim 8; and When it is evaluated that the physical state is not in the target state, information registered in the database as the following component is prompted, and the component is capable of guiding the value of the existence ratio or the parameter to one side of the numerical range displayed by the existence ratio or the parameter based on the correlation benchmark when the physical state is in the target state.

10. The use according to claim 9, wherein: Re-evaluating the physical condition of the subject after ingesting the ingredient using the evaluation according to claim 8; and The information is updated based on the degree of improvement of the re-evaluated physical condition.

11. Use of skin metabolites collected from the skin surface of a subject in the preparation of a product for screening substances for improving or preventing a physical condition, wherein: selecting the candidate substance based on the change caused by the candidate substance in the existence ratio or the value of the parameter based on the existence ratio of the skin metabolites on the skin surface for which a correlation benchmark with the physical condition has been established, The skin metabolites collected from the skin surface of the subject are obtained by collecting the skin metabolites from the skin surface of the subject using the adhesive tape according to any one of claims 1 to 3. The physical condition is: The skin condition of at least one of acne skin, skin age, oily skin, skin freckles, skin texture, yellowing, dryness, oiliness, spots, dullness, sagging, wrinkles, tension, and pores, or At least one of thinning hair, hair loss, periodontal disease, alveolar pyorrhea, menopause, liver function, high uric acid, knee pain, osteoporosis, autonomic nervous system disorder and diabetes, The correlation criterion is a criterion capable of determining the degree of goodness or severity of the physical condition to be evaluated qualitatively or quantitatively based on the value of the abundance ratio or parameter obtained from the skin metabolites.

12. A method for measuring the total amount of DNA of skin-resident microorganisms on the skin surface of a subject, the method comprising the following steps: extracting DNA from the sample collected from the skin surface using the adhesive tape according to claim 1 to prepare a DNA solution, The total amount of DNA of skin-resident microorganisms contained in the DNA solution is measured.

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