A flow-through fluorescence detection device and system

Abstract

The application relates to the technical field of medical equipment, in particular to a flow fluorescence detection device and system. The detection device at least comprises a laser emitting component, a detection component, a light filtering component, a reflecting component and a light receiving component arranged in an optical path transmission; the reflecting component is arranged on the optical path from the detection component to the light receiving component; the light filtering component is arranged on the optical path from the laser emitting component to the detection component; or the light filtering component is arranged on the optical path from the detection component to the reflecting component. The technical problem that the optical path structure is complex, the damping effect is poor and optical detection is unstable in the prior art is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical equipment, in particular to a flow fluorescence detection device and system. BACKGROUND

[0002] The flow fluorescence detector is a multi-index parallel analysis technology platform integrating flow principle, laser analysis, high-speed digital signal processing and other technologies, with fluorescent coded microspheres as the core. The sample marked by fluorescence can produce fluorescent light waves under laser excitation, and the produced fluorescent light waves have the characteristics of high sensitivity, easy distinction and easy collection. The sample can be quickly qualitatively or quantitatively analyzed, and the demand for the sample is also very small, so it is widely used in medical research and clinical trials.

[0003] Specifically, the automatic sample suction device of the flow fluorescence detector sucks the sample to be tested marked by fluorescence. The sample to be tested vertically flows through the detection pool through the pipeline. The laser is arranged in the vertical direction of the detection pool. The sample emits scattered light and fluorescent emission waves under laser excitation. The reflected light and fluorescent emission waves are filtered and reflected by a plurality of light receiving elements, and finally the information data received by the light receiving element is analyzed, processed and output by the data processing terminal.

[0004] The existing flow fluorescence detector on the market has a complex optical path structure. Before use, a complicated debugging process is required, which wastes the manpower and energy of technical personnel. Moreover, due to the complex optical path structure, the fluorescence signal is easy to lose or deviate during the detection process, thereby causing inaccurate detection results. In addition, due to the poor shock absorption effect of the existing flow fluorescence detector, the detection results of the equipment are also affected.

[0005] Therefore, the present application is proposed. SUMMARY

[0006] In view of the above technical problems, the present application provides a flow fluorescence detection device, which adopts a reasonable optical path layout, a new optical path adjustment method and a fixed shock absorption design, and can effectively improve the stability, accuracy and convenience of debugging of the optical path.

[0007] The present application provides a flow fluorescence detection device, which comprises at least a laser emitting component, a detection component, a light filtering component, a reflecting component and a light receiving component arranged in the optical path transmission. The reflecting component is arranged on the optical path from the detection component to the light receiving component. The light filtering component is arranged on the optical path from the laser emitting component to the detection component, or on the optical path from the detection component to the reflecting component.

[0008] Specifically, the flow fluorescence detection device provided by the application adopts a reasonable light path layout, effectively shortens the light path, reduces the loss of light during propagation, and thus effectively improves the stability and accuracy of the light path.

[0009] Preferably, the flow fluorescence detection device further comprises a data processing terminal for analyzing and processing the light signal received by the light receiving component, a bottom plate, and an upper cover; the laser emitting component, the detection component, the light filtering component, the reflecting component, the light receiving component, and the data processing terminal are arranged on the bottom plate; and the bottom plate is fixedly connected with the upper cover.

[0010] In the above structure, the laser emitting component, the detection component, the light filtering component, the reflecting component, the light receiving component, and the data processing terminal are arranged on the bottom plate, and the bottom plate supports and fixes the entire light path system. The bottom plate is fixedly connected with the upper cover, and a containing chamber is formed between the bottom plate and the upper cover. The laser emitting component, the detection component, the light filtering component, the reflecting component, the light receiving component, and the data processing terminal are located in the containing chamber, so that the bottom plate and the upper cover can prevent dust and protect the entire light path system. The data processing terminal is in communication connection with the light receiving component. After the data processing terminal receives the data information generated by the light signal collected by the light receiving component, the data processing terminal analyzes and processes the data information, and sends the processing result to the display terminal for display.

[0011] Preferably, the laser emitting component comprises a first laser and a second laser; the first laser and the second laser are provided with a light path coupling component between the first laser and the second laser and the detection component; the light path coupling component comprises an upper cover, a sealing gasket, and a light path coupling component shell, and the light path coupling component shell comprises a trapezoidal frame and first and second plate surfaces arranged in the trapezoidal frame.

[0012] The first laser and the second laser are located opposite to the lower bottom surface of the trapezoidal frame, and the laser emitted by the first laser and the second laser can enter the light path coupling component through the light filtering sheet arranged on the lower bottom surface, and is coupled through the reflecting sheet and the convex lens sheet arranged in the light path coupling component, and is emitted from the light filtering sheet arranged on the upper bottom surface of the trapezoidal frame.

[0013] In the structure, the top of the light path coupling component housing is provided with an opening, and sealing is realized through the upper cover and the sealing gasket. The upper cover can not only protect the internal devices of the light path coupling component housing, but also has a certain dustproof effect. The sealing gasket arranged between the upper cover and the light path coupling component housing has the functions of further dustproof and light shielding. The trapezoidal frame includes an upper bottom surface, a lower bottom surface, a first side surface and a second side surface. The first plate surface and the second plate surface are fixedly connected. The free end of the first plate surface is fixed at the connection between the first side surface and the upper bottom surface of the trapezoidal frame. The free end of the second plate surface is fixed at the lower bottom surface of the trapezoidal structure. The first laser and the second laser are arranged side by side. The light outlets of the first laser and the second laser both face the lower bottom surface of the trapezoidal frame of the light path coupling component. The filter of the light path coupling component includes a first coupling filter, a second coupling filter and a third coupling filter. The first coupling filter and the second coupling filter are arranged on the lower bottom surface of the trapezoidal frame. The third coupling filter is arranged on the upper bottom surface of the trapezoidal frame. The reflecting sheet of the light path coupling component includes a first coupling reflecting sheet, a second coupling reflecting sheet and a third coupling reflecting sheet. The first coupling reflecting sheet is arranged on the first side surface. The second coupling reflecting sheet is arranged on the second plate surface. The third coupling reflecting sheet is arranged on the second side surface. The convex lens is arranged on the first plate surface.

[0014] When the detection device is turned on, the laser emitted by the first laser enters the light path coupling component through the first coupling filter arranged on the lower bottom surface of the trapezoidal frame. After being reflected by the first coupling reflecting sheet and the second coupling reflecting sheet arranged on the first side surface and the second plate surface, the laser is transmitted through the convex lens arranged on the first plate surface. The laser emitted by the second laser enters the light path coupling component through the second coupling filter arranged on the lower bottom surface of the trapezoidal frame. After being reflected by the third coupling reflecting sheet arranged on the second side surface and the convex lens arranged on the first plate surface, the laser is coupled with the laser emitted by the first laser and transmitted through the convex lens. The coupled laser is transmitted out of the light path coupling component through the third filter arranged on the upper bottom surface of the trapezoidal frame, so as to realize the coupling of the lasers emitted by the first laser and the second laser. The coupled laser can enter the detection component, excite the sample to be measured marked by fluorescence in the detection component, and thus generate fluorescence.

[0015] Preferably, the number of reflecting components is multiple, and at least arranged on the light path from the detection component to the light receiving component. The reflecting component includes a reflecting sheet, a reflecting sheet fixing plate, a reflecting sheet supporting plate, an elastic assembly, an elastic assembly fixing piece and a fixing plate adjusting piece. The reflecting sheet is arranged on the reflecting sheet fixing plate. The elastic assembly passes through the reflecting sheet fixing plate and the reflecting sheet supporting plate, and the two ends are fixed by the elastic assembly fixing piece. The fixing plate adjusting piece passes through the reflecting sheet supporting plate and abuts against the reflecting sheet fixing plate.

[0016] In the scheme, at least the setting should be understood as, in addition to the light path between the detection component and the light receiving component is provided with a reflecting component, the reflecting component can also be provided on the other light path. Specifically, the reflecting component at least includes a first reflecting component and a second reflecting component, the first reflecting component and the second reflecting component are arranged on both sides of the detection component and located on the light path between the detection component and the light receiving component. The first reflecting component and the second reflecting component are the same structure. The first reflecting component at least includes two elastic components, the elastic component can be a tension spring, the reflecting sheet fixing plate and the reflecting sheet support plate are fixed by the tension spring, which not only can ensure the stability of the overall structure, but also because the tension spring has elasticity, it is also convenient to adjust the relative position of the reflecting sheet fixing plate and the reflecting sheet support plate. At least including three fixed plate adjusting pieces, the fixed plate adjusting piece can be a top screw, specifically, the top screw passes through the threaded hole on the reflecting sheet support plate, and is supported in the groove of the reflecting sheet fixing plate, and is triangularly distributed. Because the reflecting sheet fixing plate and the reflecting sheet support plate are fixed by the tension spring arranged through, the inclination angle of the reflecting sheet fixing plate can be adjusted by adjusting the depth of the top screw screwing in, that is, the inclination angle of the reflecting sheet is adjusted, and then the direction of the fluorescent signal after reflecting by the reflecting sheet can be adjusted. In addition, the design of the triangular distribution of the top screw not only can adjust the inclination angle of the reflecting sheet to the greatest extent, but also can adjust the inclination angle of the reflecting sheet in each direction.

[0017] Preferably, the number of light filtering components is multiple, and at least is arranged on the light path from the laser emitting component to the detection component and the light path from the detection component to the reflecting component; the light filtering component includes a lens fixing plate, a lens support plate, a lens, a lens fixing piece, an elastic component, an elastic component fixing piece and a fixed plate adjusting piece; the lens is arranged on the lens fixing plate, protrudes from both ends of the lens fixing plate, and is fixed by the lens fixing piece; the elastic component passes through the lens fixing plate and the lens support plate, and both ends are fixed by the elastic component fixing piece, one end of the lens is located in the through hole arranged on the lens support plate; the fixed plate adjusting piece passes through the lens support plate and abuts against the lens fixing plate.

[0018] In the scheme, at least the setting should be understood as, in addition to the light path between the detection component and the light receiving component is provided with a reflecting component, the reflecting component can also be provided on the other light path. Specifically, the reflecting component at least includes a first reflecting component and a second reflecting component, the first reflecting component and the second reflecting component are arranged on both sides of the detection component and located on the light path between the detection component and the light receiving component. The first reflecting component and the second reflecting component are the same structure. The first reflecting component at least includes two elastic components, the elastic component can be a tension spring, the reflecting sheet fixing plate and the reflecting sheet support plate are fixed by the tension spring, which not only can ensure the stability of the overall structure, but also because the tension spring has elasticity, it is also convenient to adjust the relative position of the reflecting sheet fixing plate and the reflecting sheet support plate. At least including three fixed plate adjusting pieces, the fixed plate adjusting piece can be a top screw, specifically, the top screw passes through the threaded hole on the reflecting sheet support plate, and is supported in the groove of the reflecting sheet fixing plate, and is triangularly distributed. Because the reflecting sheet fixing plate and the reflecting sheet support plate are fixed by the tension spring arranged through, the inclination angle of the reflecting sheet fixing plate can be adjusted by adjusting the depth of the top screw screwing in, that is, the inclination angle of the reflecting sheet is adjusted, and then the direction of the fluorescent signal after reflecting by the reflecting sheet can be adjusted. In addition, the design of the triangular distribution of the top screw not only can adjust the inclination angle of the reflecting sheet to the greatest extent, but also can adjust the inclination angle of the reflecting sheet in each direction.

[0019] The first filter component, the second filter component and the third filter component are of the same structure, the first filter component comprises at least two elastic components, the elastic components can be tension springs, after the lens fixing plate and the lens supporting plate are fixed by the tension springs, the stability of the overall structure can be ensured, and since the tension springs are elastic, the relative positions of the lens fixing plate and the lens supporting plate can be conveniently adjusted subsequently. The lens fixing plate comprises at least three fixing plate adjusting pieces, the fixing plate adjusting pieces can be jacks, specifically, the jacks are inserted into the grooves of the lens fixing plate through the threaded through holes on the lens supporting plate and are distributed in a triangular shape, since the lens fixing plate and the lens supporting plate are fixed by the tension springs arranged through the lens fixing plate and the lens supporting plate, the inclination angle of the lens fixing plate can be adjusted by adjusting the depths to which the jacks are screwed, that is, the inclination angle of the lens is adjusted, so that the coupled laser or the fluorescence generated by the laser exciting the sample to be detected can be transmitted through the lens; in addition, the jacks are distributed in a triangular shape, so that the inclination angle of the lens can be adjusted to the greatest extent, and the inclination angle of the lens in each direction can be adjusted.

[0020] Preferably, the flow fluorescence detection device further comprises a light measuring component, the light measuring component is arranged on the light path between the reflecting component and the light receiving component; the light measuring component comprises a light measuring fixing plate, a double-plate supporting plate, a light measuring reflecting piece, an elastic component, an elastic component fixing piece, a fixing plate adjusting piece and a light measuring component housing; the light measuring fixing plate is provided with a convex structure, the convex structure is provided with an inclined surface and a through hole penetrating through the inclined surface, and the light measuring reflecting piece is arranged on the inclined surface; the elastic component penetrates through the light measuring fixing plate and the double-plate supporting plate and is fixed at both ends by the elastic component fixing piece; the fixing plate adjusting piece penetrates through the double-plate supporting plate and abuts against the light measuring fixing plate; and the light measuring component housing is fixedly connected with the double-plate supporting plate.

[0021] In the scheme, the light measuring component is arranged on the light path of the fluorescent light reflected by the reflecting component. Specifically, the light measuring fixed plate includes a first light measuring fixed plate and a second light measuring fixed plate, and the first light measuring fixed plate and the second light measuring fixed plate are both provided with a protruding structure. One section of the protruding structure can be a structure with one inclined surface such as a right trapezoid or a right triangle, and a through hole is arranged on the protruding structure and penetrates the inclined surface and the adjacent surface thereof. The light measuring reflecting sheet includes a first light measuring reflecting sheet and a second light measuring reflecting sheet, and the first light measuring reflecting sheet and the second light measuring reflecting sheet are arranged on the inclined surfaces of the protruding structures of the first light measuring reflecting sheet and the second light measuring reflecting sheet respectively. The light measuring component includes at least four elastic components, and the elastic component can be a tension spring. After the first light measuring fixed plate and the double-plate supporting plate are fixed by at least two tension springs, the stability of the overall structure can be ensured, and since the tension spring has elasticity, the relative position of the first light measuring fixed plate and the double-plate supporting plate can be adjusted subsequently. The light measuring component includes at least six fixed plate adjusting pieces, and the fixed plate adjusting piece can be a jackscrew. Specifically, at least three jackscrews are arranged in the grooves of the first light measuring fixed plate through the threaded through holes on the double-plate supporting plate, and the at least three jackscrews are distributed in a triangular shape. Since the first light measuring fixed plate and the double-plate supporting plate are fixed by the penetrating tension springs, the inclination angle of the first light measuring fixed plate can be adjusted by adjusting the depth of the jackscrews, that is, the inclination angle of the first light measuring reflecting sheet is adjusted, so that the fluorescent light generated by the coupling of the laser exciting the sample to be measured can be transmitted through the light measuring reflecting sheet or reflected by the light measuring reflecting sheet. In addition, the design of the triangular distribution of the jackscrews can adjust the inclination angle of the light measuring reflecting sheet to the greatest extent, and can adjust the inclination angle of the light measuring reflecting sheet in each direction. In addition, the fixing mode of the second light measuring fixed plate and the arrangement mode of the jackscrews are the same as those of the first light measuring fixed plate, and the first light measuring fixed plate and the second light measuring fixed plate are sequentially fixed on the light path of the fluorescent light by the tension springs. A plurality of through holes are arranged on the light measuring component shell, so that the fluorescent light can enter the light measuring component, and the fluorescent light reflected by the first light measuring reflecting sheet and the second light measuring reflecting sheet and sequentially transmitted through the first light measuring reflecting sheet and the second light measuring reflecting sheet can be emitted from the light measuring component.

[0022] Preferably, the number of light receiving components is multiple, and at least arranged on the light path after the reflecting component; or, on the light path after the light measuring component.

[0023] In the scheme, at least it is understood that, in addition to the light path after the reflection component and the light measuring component is provided with light receiving component, light receiving component can also be set on other light path. The light receiving component at least includes first light receiving component, second light receiving component, third light receiving component and fourth light receiving component, the first light receiving component is set on the light path after the reflection component, for receiving the fluorescent signal reflected by the reflection component, the second light receiving component is set on the light path after the light measuring component, for receiving the fluorescent signal transmitted through the first light measuring reflection sheet and the second light measuring reflection sheet in turn, the third light receiving component and the fourth light receiving component are both set on the light path after the light measuring component, for receiving the fluorescent signal reflected by the first light measuring reflection sheet and the second light measuring reflection sheet respectively.

[0024] Preferably, the detection component includes a detection pool, a pipeline and a pipeline support; the detection pool and the pipeline support are both arranged on the bottom plate, one end of the pipeline passes through the pipeline support and is connected with the detection pool, and the other end passes through the bottom plate and is connected with an external liquid path.

[0025] In the above structure, the pipeline support supports and fixes the pipeline, the detection pool and the pipeline are connected to form a liquid path inside the flow fluorescence detection device, the detection pool is funnel-shaped, one end of which has a large diameter and the other end has a small diameter, and the end with the small diameter is connected with the pipeline; the sample to be measured enters from the end with the large diameter, and the sample to be measured is subjected to pressure from the surrounding wall due to the gradually reduced diameter of the path in the detection pool, so that the fluorescently labeled DNA or cell or particle suspended in the sample to be measured passes through the detection pool and can generate fluorescence after being excited by the laser from the vertical direction.

[0026] Preferably, the bottom plate is a rectangular plate, a shock-absorbing pad is arranged at the top corner of the bottom plate or the edge of the bottom plate, and the top corner of the bottom plate is rounded.

[0027] In the above structure, the shock-absorbing pad is a cylindrical structure, the shock-absorbing pad is arranged through the thickness direction of the bottom plate, the axial cross section of the shock-absorbing pad is in the shape of an I-beam, the height of the recessed side of the I-beam is adapted to the thickness of the bottom plate, and the shock-absorbing pad is further provided with a through hole in the axial direction, so that the shock-absorbing pad can be fixed on the bottom plate by a fastener. The rounded top corner of the bottom plate not only looks good, but also can avoid scratching other equipment or experimental personnel.

[0028] Preferably, the flow fluorescence detection device further comprises a fan and a fan support; one end of the bottom plate is provided with the fan support, and the fan is installed on the fan support; the other end of the bottom plate is provided with a wire outlet.

[0029] In the structure, the fan and the outlet are located at two ends of the bottom plate, so that a long air circulation path is formed, and the laser emitting component, the light filtering component, the detection component, the reflecting component, the light measuring component and the light receiving component are located on the air circulation path, so that the laser emitting component, the light filtering component, the detection component, the reflecting component, the light measuring component and the light receiving component can be cooled conveniently.

[0030] Preferably, the flow fluorescence detection device further comprises a first laser fixing plate and a baffle plate with a through hole, the first laser is arranged on the bottom plate through the first laser fixing plate, and the baffle plate is arranged on the front side of the first laser, and the laser emitted by the first laser can be transmitted through the through hole of the baffle plate.

[0031] Preferably, dustproof cotton is arranged between the detection pool and the bottom plate, dustproof cotton is arranged between the pipeline and the bottom plate, and dustproof cotton is arranged at the outlet.

[0032] In the structure, the detection pool and the pipeline are arranged on the bottom plate in a penetrating manner instead of being connected in a closed manner, so that a gap is formed between the detection pool, the pipeline and the bottom plate, and the outlet is a hole through which the detection device communicates with the outside, so that dust and the like cannot enter the detection device, thereby affecting the normal use of the components, and therefore, dustproof cotton is arranged between the detection pool and the bottom plate, between the pipeline and the bottom plate and at the outlet.

[0033] The application provides a flow fluorescence detection system, which comprises an automatic sample suction device, a power device, a control device, a box body and the flow fluorescence detection device.

[0034] In the scheme, the flow fluorescence detection device, the automatic sample suction device, the power device and the control device are arranged on the box body, so that the box body can fix and protect the devices, and in addition, the automatic sample suction device and the like which should protrude from the side of the box body do not protrude from the side of the box body through the recess structure arranged on the box body, so that the devices are better protected. The flow fluorescence detection device and the automatic sample suction device are in liquid communication, and under the driving of the power device, the automatic sample suction device and the flow fluorescence detection device can complete the suction and detection of the sample to be detected.

[0035] In summary, the application provides a flow fluorescence detection device and system, the detection device adopts reasonable light path layout, new light path adjustment mode and fixed damping design, can effectively improve the stability, accuracy of light path and convenience of debugging, in addition, the detection system has high automation degree, simple structure, small size, fast detection speed, and can detect multiple items at the same time, thereby solving the technical problems of complex light path structure, poor damping effect and unstable optical detection in the prior art. BRIEF DESCRIPTION OF DRAWINGS

[0036] In order to more clearly illustrate the specific embodiments of the application or the technical solutions in the prior art, the drawings needed in the specific embodiments or the prior art description will be briefly introduced below, and obviously, the drawings in the following description are some embodiments of the application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0037] Figure 1 A structural schematic diagram of a flow fluorescence detection system provided by an embodiment of the application is shown in the figure.

[0038] Figure 2 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0039] Figure 3 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0040] Figure 4 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0041] Figure 5 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0042] Figure 6 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0043] Figure 7 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0044] Figure 8 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0045] Figure 9 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0046] Figure 10 A structural schematic diagram of a flow fluorescence detection device provided by an embodiment of the application is shown in the figure.

[0047] Figure 11 The structure diagram of the light measuring component provided by the embodiment of the present application is shown.

[0048] In the drawings, the components represented by the respective reference numerals are as follows:

[0049] 1, bottom plate; 2, upper cover; 3, first laser; 4, second laser; 5, light path coupling component; 6, first reflecting component; 7, second reflecting component; 8, light measuring component; 9, first light filtering component; 10, second light filtering component; 11, third light filtering component; 12, first light receiving component; 13, second light receiving component; 14, third light receiving component; 15, fourth light receiving component; 16, detection cell; 17, pipeline support; 18, pipeline; 19, data processing terminal; 20, fan support; 21, fan; 22, first laser fixing plate; 23, baffle; 24, support column; 25, shock pad; 26, outlet; 27, tension spring; 28, pin shaft; 29, jackscrew; 501, light path coupling component housing; 502, sealing pad; 503, upper cover; 504, upper bottom surface; 505, lower bottom surface; 506, first side surface; 507, second side surface; 508, first plate surface; 509, second plate surface; 510, first coupling filter; 511, second coupling filter; 512, third coupling filter; 513, first coupling reflecting sheet; 514, second coupling reflecting sheet; 515, third coupling reflecting sheet; 516, convex lens sheet; 601, reflecting sheet; 602, reflecting sheet fixing plate; 603, reflecting sheet support plate; 801, first light measuring fixing plate; 802, second light measuring fixing plate; 803, double sheet support plate; 804, first light measuring reflecting sheet; 805, second light measuring reflecting sheet; 901, lens; 902, lens fixing plate; 903, lens support plate; 904, fixing nut; 101, flow cytometry detection device; 102, automatic sample suction device; 103, control device; 104, power device; 105, box body. DETAILED DESCRIPTION

[0050] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0051] In addition, the terms "first", "second", etc. are used herein only to describe different instances, and are not used to denote or imply relative importance or a number of indications of the technical features indicated. Thus, the technical features defined with "first", "second", etc. can explicitly or implicitly include at least one of the technical features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0052] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0053] In the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "under" and "under" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0054] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present application and the features of different embodiments or examples, without contradiction.

[0055] Please refer to Figures 2-11In one embodiment of the present application, a flow fluorescence detection device 101 is provided, which comprises at least a laser emitting component, a detection component, a filter component, a reflection component and a light receiving component arranged in the light path; the reflection component is arranged in the light path from the detection component to the light receiving component; the filter component is arranged in the light path from the laser emitting component to the detection component; or the filter component is arranged in the light path from the detection component to the reflection component.

[0056] As a further preferred embodiment, on the basis of the above-mentioned scheme, the specific embodiment of the present application can further comprise one or more of the following additions or combinations:

[0057] The flow fluorescence detection device 101 further comprises a data processing terminal 19 for analyzing and processing the light signal received by the light receiving component, a bottom plate 1 and an upper cover 2; the laser emitting component, the detection component, the filter component, the reflection component, the light receiving component and the data processing terminal 19 are all arranged on the bottom plate 1; the bottom plate 1 is fixedly connected with the upper cover 2.

[0058] The laser emitting component comprises a first laser 3 and a second laser 4; the first laser 3 and the second laser 4 are provided with a light path coupling component 5 between the first laser 3 and the second laser 4 and the detection component; the light path coupling component 5 comprises an upper cover 503, a sealing gasket 502 and a light path coupling component housing 501, the light path coupling component housing 501 comprises a trapezoidal frame and a first plate surface 508 and a second plate surface 509 arranged inside the trapezoidal frame, the first plate surface 508 and the second plate surface 509 are fixedly connected, the free end of the first plate surface 508 is fixed at the connection between the side surface of the trapezoidal frame and the upper bottom surface 504, and the free end of the second plate surface 509 is fixed at the lower bottom surface 505 of the trapezoidal structure; the positions of the first laser 3 and the second laser 4 are opposite to the lower bottom surface 505 of the trapezoidal frame, the laser emitted by the first laser 3 and the second laser 4 can enter the light path coupling component 5 through the filter arranged on the lower bottom surface 505 of the trapezoidal frame, and be coupled through the reflecting sheet and the convex lens sheet 516 arranged inside the light path coupling component 5, and be emitted through the filter arranged on the upper bottom surface 504 of the trapezoidal frame.

[0059] The number of reflection components is multiple, and at least arranged in the light path from the detection component to the light receiving component; the reflection component comprises a reflecting sheet 601, a reflecting sheet fixing plate 602, a reflecting sheet support plate 603, an elastic assembly, an elastic assembly fixing member and a fixing plate adjusting member; the reflecting sheet 601 is arranged on the reflecting sheet fixing plate 602; the elastic assembly passes through the reflecting sheet fixing plate 602 and the reflecting sheet support plate 603, and the two ends are fixed by the elastic assembly fixing member; the fixing plate adjusting member passes through the reflecting sheet support plate 603 and is arranged in the groove on the reflecting sheet fixing plate 602.

[0060] The number of light filtering components is multiple, and at least arranged on the light path from the laser emitting component to the detecting component and on the light path from the detecting component to the reflecting component; the light filtering components comprise a lens fixing plate 902, a lens supporting plate 903, a lens 901, a lens 901 fixing element, an elastic assembly, an elastic assembly fixing element, and a fixing plate adjusting element; the lens 901 is arranged on the lens fixing plate 902, protrudes from both ends of the lens fixing plate 902, and is fixed by the lens 901 fixing element; the elastic assembly passes through the lens fixing plate 902 and the lens supporting plate 903, and is fixed by the elastic assembly fixing element at both ends, and one end of the lens 901 is located in the through hole arranged on the lens supporting plate 903; the fixing plate adjusting element passes through the lens supporting plate 903 and is located in the groove arranged on the lens fixing plate 902.

[0061] The flow fluorescence detection device 101 further comprises a light measuring component 8 arranged on the light path between the reflecting component and the light receiving component; the light measuring component 8 comprises a light measuring fixing plate, a double-plate supporting plate 803, a light measuring reflecting sheet, an elastic assembly, an elastic assembly fixing element, a fixing plate adjusting element, and a light measuring component housing; the light measuring fixing plate is provided with a protruding structure, the protruding structure is provided with an inclined surface and a through hole penetrating through the inclined surface, and the light measuring reflecting sheet is arranged on the inclined surface; the elastic assembly passes through the light measuring fixing plate and the double-plate supporting plate 803, and is fixed by the elastic assembly fixing element at both ends; the fixing plate adjusting element passes through the double-plate supporting plate 803 and abuts against the light measuring fixing plate; and the light measuring component housing is fixedly connected with the double-plate supporting plate 803.

[0062] The number of light receiving components is multiple, and at least arranged on the light path after the reflecting component; or, on the light path after the light measuring component 8.

[0063] The detecting component comprises a detecting pool 16, a pipeline 18, and a pipeline support 17; the detecting pool 16 and the pipeline support 17 are both arranged on the bottom plate 1, one end of the pipeline 18 is connected with the detecting pool 16 by passing through the pipeline support 17, and the other end is connected with an external liquid path by passing through the bottom plate 1.

[0064] The bottom plate 1 is a rectangular plate, a damping pad 25 is arranged at a top corner position of the bottom plate 1 or an edge of the bottom plate 1, and the top corner of the bottom plate 1 is rounded.

[0065] The flow fluorescence detection device 101 further comprises a fan 21 and a fan support 20; one end of the bottom plate 1 is provided with the fan support 20, and the fan 21 is installed on the fan support 20; the other end of the bottom plate 1 is provided with a wire outlet 26.

[0066] The flow fluorescence detection device 101 further comprises a first laser fixing plate 22 and a baffle 23 provided with a through hole; the first laser 3 is arranged on the bottom plate 1 by the first laser fixing plate 22, the baffle 23 is arranged on the front side of the first laser 3, and the laser emitted by the first laser 3 can be transmitted through the through hole of the baffle 23.

[0067] The detection pool 16 is provided with detection pool 16 dustproof cotton between the bottom plate 1, the pipeline 18 is provided with liquid path pipe dustproof cotton between the bottom plate 1, and the outlet port 26 is provided with outlet port dustproof cotton.

[0068] Please refer to Figure 1 In one embodiment of the present application, a flow fluorescence detection system is provided, which comprises an automatic sample suction device 102, a power device 104, a control device 103, a box 105 and the flow fluorescence detection device 101 in the above embodiment; the flow fluorescence detection device 101, the automatic sample suction device 102, the power device 104 and the control device 103 are all arranged on the box 105; the control device 103 is in communication connection with the power device 104; the power device 104 is in electrical connection with the flow fluorescence detection device 101 and the automatic sample suction device 102; the flow fluorescence detection device 101 is in liquid connection with the automatic sample suction device 102.

[0069] Please refer to Figures 2-11 In some preferred embodiments of the present application, the flow fluorescence detection device 101 comprises a laser emitting component, a detection component, a light filtering component, a reflecting component, a light measuring component 8, a light receiving component, a data processing terminal 19 for analyzing and processing the light signal received by the light receiving component, a bottom plate 1, an upper cover 2, a support column 24 and a shock pad 25; the laser emitting component, the detection component, the light filtering component, the reflecting component, the light receiving component, the data processing terminal 19, the support column 24 and the shock pad 25 are all arranged on the bottom plate 1, and the bottom plate 1 supports and fixes the entire optical path system. The bottom plate 1 is fixedly connected with the upper cover 2 through the support column 24, and a containing chamber is formed between the bottom plate 1 and the upper cover 2, and the laser emitting component, the detection component, the light filtering component, the reflecting component, the light measuring component 8, the light receiving component and the data processing terminal 19 are all located in the containing chamber, so that the bottom plate 1 and the upper cover 2 can prevent dust and protect the entire optical path system. The data processing terminal 19 is in communication connection with the light receiving component, and after receiving the data information generated by the light signal collected by the light receiving component, the data processing terminal 19 analyzes and processes the data information and sends the processing result to the display terminal for display.

[0070] The flow fluorescence detection device 101 further comprises a first laser fixing plate 22, a baffle 23 with a through hole, a fan 21 and a fan support 20; specifically, the laser emitting component comprises a first laser 3 and a second laser 4; the reflecting component comprises a first reflecting component 6 and a second reflecting component 7, the light filtering component comprises a first light filtering component 9, a second light filtering component 10 and a third light filtering component 11, the light receiving component comprises a second light receiving component 13, a third light receiving component 14 and a fourth light receiving component 15, the detection component comprises a detection pool 16, a pipeline 18 and a pipeline support 17, the light path coupling component 5 comprises an upper cover 503, a sealing gasket 502 and a light path coupling component housing 501, and the light path coupling component housing 501 comprises a trapezoidal frame and a first plate surface 508 and a second plate surface 509 arranged inside the trapezoidal frame.

[0071] Specifically, taking the detection pool 16 as the center, the light path coupling component 5 is arranged on the back side of the detection pool 16, and the side of the upper base of the trapezoidal frame faces the detection pool 16, and the first light filtering component 9 is arranged between the detection pool 16 and the light path coupling component 5; the first laser 3 and the second laser 4 are arranged side by side on the back side of the light path coupling component 5, and the first laser 3 is arranged on the bottom plate 1 through the first laser fixing plate 22, and the front side of the first laser 3 is provided with the baffle 23 with a through hole, and the laser emitted by the first laser 3 can transmit through the through hole of the baffle 23, and the light outlets of the first laser 3 and the second laser 4 face the side of the lower base of the trapezoidal frame; the data processing terminal 19 is arranged on the back side of the second laser 4; the second light filtering component 10 and the third light filtering component 11 are arranged vertically to the light path coupling component 5 on the left side and the right side of the detection pool 16 respectively; the first reflecting component 6 is arranged on the back side of the second light filtering component 10, and forms a 45° angle with the second light filtering component 10, and the second reflecting component 7 is arranged on the back side of the third light filtering component 11, and forms a 45° angle with the third light filtering component 11; the first light receiving component 12 is arranged on the left front side of the detection pool 16, and is used for receiving the reflected fluorescence of the first reflecting component 6; the light measuring component 8 is arranged on the right front side of the detection pool 16, and is used for transmitting or reflecting the reflected fluorescence of the second reflecting component 7; the second light receiving component 13 is arranged on the front side of the light measuring component 8, and the third light receiving component 14 and the fourth light receiving component 15 are arranged side by side on the left side of the light measuring component 8, and the second light receiving component 13, the third light receiving component 14 and the fourth light receiving component 15 are used for receiving the fluorescence passing through the light measuring component 8; the pipeline support 17 is arranged above the detection pool 16, one end of the pipeline 18 is connected with the detection pool 16 through the pipeline support 17, and the other end of the pipeline 18 passes out from the bottom plate 1 on the front side of the detection pool 16; the fan 21 and the fan support 20 are arranged on the edge of the bottom plate 1 on the front side of the detection pool 16, and the data processing terminal 19 is provided with a wire outlet 26 on the back side of the bottom plate 1.

[0072] Further, the fan 21 and the outlet 26 are respectively located at two ends of the bottom plate 1, so that a long air flow path is formed, and the laser emitting component, the light filtering component, the detecting component, the reflecting component, the light measuring component 8 and the light receiving component are located on the air flow path, so that the laser emitting component, the light filtering component, the detecting component, the reflecting component, the light measuring component 8 and the light receiving component are conveniently cooled.

[0073] Further, the light path coupling component 5 further comprises a first coupling filter 510, a second coupling filter 511, a third coupling filter 512, a first coupling reflecting sheet 513, a second coupling reflecting sheet 514, a third coupling reflecting sheet 515 and a convex lens 516. The trapezoidal frame comprises an upper bottom surface 504, a lower bottom surface 505, a first side surface 506 and a second side surface 507. The first plate surface 508 and the second plate surface 509 are fixedly connected. The free end of the first plate surface 508 is fixed at the connection between the first side surface 506 and the upper bottom surface 504, and the free end of the second plate surface 509 is fixed at the lower bottom surface 505. The first coupling filter 510 and the second coupling filter 511 are arranged on the lower bottom surface 505 of the trapezoidal frame, and the third coupling filter 512 is arranged on the upper bottom surface 504 of the trapezoidal frame. The first coupling reflecting sheet 513 is arranged on the first side surface 506, the second coupling reflecting sheet 514 is arranged on the second plate surface 509, the third coupling reflecting sheet 515 is arranged on the second side surface 507, and the convex lens 516 is arranged on the first plate surface 508.

[0074] When the detection device is turned on, the laser emitted by the first laser 3 enters the light path coupling component 5 through the first coupling filter 510 arranged on the lower bottom surface 505, is reflected by the first coupling reflecting sheet 513 and the second coupling reflecting sheet 514 arranged on the first side surface 506 and the second plate surface 509, and is transmitted through the convex lens 516 arranged on the first plate surface 508. The laser emitted by the second laser 4 enters the light path coupling component 5 through the second coupling filter 511 arranged on the lower bottom surface 505 of the trapezoidal frame, is reflected by the third coupling reflecting sheet 515 arranged on the second side surface 507 and the convex lens 516 arranged on the first plate surface 508, and is coupled with the laser emitted by the first laser 3 and transmitted through the convex lens 516. The coupled laser is transmitted out of the light path coupling component 5 through the third filter arranged on the upper bottom surface 504, so that the coupling of the lasers emitted by the first laser 3 and the second laser 4 is realized. After being transmitted through the first light filtering component 9, the coupled laser irradiates on the detection pool 16, excites the sample to be measured which is marked by fluorescence in the detection pool 16, so that fluorescence is generated.

[0075] Further, the first reflecting component 6 and the second reflecting component 7 are structurally identical. The first reflecting component 6 comprises a reflector 601, a reflector fixing plate 602, a reflector supporting plate 603, two tension springs 27, four pin shafts 28 and three jackscrews 29; the reflector 601 is arranged on the reflector fixing plate 602; the tension springs 27 pass through the reflector fixing plate 602 and the reflector supporting plate 603, and the two ends are fixed by the pin shafts 28; the jackscrews 29 pass through the reflector supporting plate 603 and are arranged in the grooves provided on the reflector fixing plate 602. The reflector fixing plate 602 and the reflector supporting plate 603 are fixed by the tension springs 27, which not only ensures the stability of the overall structure, but also facilitates the subsequent adjustment of the relative position of the reflector fixing plate 602 and the reflector supporting plate 603 due to the elasticity of the tension springs 27. The three jackscrews 29 are triangularly distributed. Since the reflector fixing plate 602 and the reflector supporting plate 603 are fixed by the tension springs 27, the inclination angle of the reflector fixing plate 602, and thus the inclination angle of the reflector 601, can be adjusted by adjusting the depth of the jackscrews 29, thereby adjusting the direction of the fluorescent signal after being reflected by the reflector 601. In addition, the triangular distribution of the jackscrews 29 not only allows for a large adjustment range of the inclination angle of the reflector 601, but also allows for adjustment of the inclination angle of the reflector 601 in various directions.

[0076] Further, the first filter component 9, the second filter component 10 and the third filter component 11 are of the same structure, the first filter component 9 comprises a lens fixing plate 902, a lens support plate 903, a lens 901, a fixing nut 904, two tension springs 27, four pin shafts 28 and three jackscrews 29; the lens 901 is arranged on the lens fixing plate 902, protrudes from both ends of the lens fixing plate 902 and is fixed by the fixing nut 904; the tension spring 27 passes through the lens fixing plate 902 and the lens support plate 903, is fixed by the pin shaft 28 at both ends and one end of the lens 901 is arranged in a through hole on the lens support plate 903; the jackscrew 29 passes through the lens support plate 903 and is arranged in a groove on the lens fixing plate 902. After the lens fixing plate 902 and the lens support plate 903 are fixed by the tension spring 27, the stability of the overall structure can be ensured, and since the tension spring 27 is elastic, the relative position of the lens fixing plate 902 and the lens support plate 903 can be adjusted subsequently. The jackscrews 29 are distributed in a triangular shape, since the lens fixing plate 902 and the lens support plate 903 are fixed by the tension spring 27 arranged therethrough, the inclination angle of the lens fixing plate 902, that is, the inclination angle of the lens 901 can be adjusted by adjusting the depth of the jackscrews 29 screwed in respectively, so that the coupled laser or the fluorescence generated by the laser exciting the sample under test can be transmitted through the lens 901; in addition, the design of the jackscrews 29 distributed in a triangular shape can not only adjust the inclination angle of the lens 901 to a large extent, but also adjust the inclination angle of the lens 901 in each direction.

[0077] Further, the light measuring component 8 comprises a first light measuring fixed plate 801, a second light measuring fixed plate 802, a double-plate supporting plate 803, a first light measuring reflector 804, a second light measuring reflector 805, four tension springs 27, eight pin shafts 28, six jackscrews 29, and a light measuring component housing; the first light measuring fixed plate 801 and the second light measuring fixed plate 802 are both provided with protruding structures, one section of the protruding structure is a right trapezoid, and the protruding structure is provided with a through hole penetrating through the inclined surface and the adjacent surface. The light measuring reflectors comprise the first light measuring reflector 804 and the second light measuring reflector 805, and the first light measuring reflector 804 and the second light measuring reflector 805 are respectively arranged on the inclined surface of the protruding structure of the first light measuring fixed plate 801 and the second light measuring fixed plate 802. After the first light measuring fixed plate 801 is fixed with the double-plate supporting plate 803 through the two tension springs 27, the stability of the overall structure can be ensured, and since the tension springs 27 are elastic, the relative position of the first light measuring fixed plate 801 and the double-plate supporting plate 803 can also be adjusted subsequently. The three jackscrews 29 are penetrated through the threaded through holes on the double-plate supporting plate 803 and are jacked in the grooves of the first light measuring fixed plate 801, and are distributed in a triangular shape. Since the first light measuring fixed plate 801 and the double-plate supporting plate 803 are fixed through the penetrated tension springs 27, the inclination angle of the first light measuring fixed plate 801, that is, the inclination angle of the light measuring reflector, can be adjusted by respectively adjusting the depth of the jackscrews 29, so that the fluorescence generated by the coupling of the laser excitation sample can be transmitted through the light measuring reflector or reflected by the light measuring reflector. In addition, the design of the triangular distribution of the jackscrews 29 can not only adjust the inclination angle of the light measuring reflector to a great extent, but also adjust the inclination angle of the light measuring reflector in each direction. The fixing mode of the second light measuring fixed plate 802 and the setting mode of the jackscrews 29 are the same as those of the first light measuring fixed plate 801 and the jackscrews 29, and the first light measuring fixed plate 801 and the second light measuring fixed plate 802 are sequentially fixed on the light path of the fluorescence through the tension springs 27. The light measuring component housing is provided with a plurality of through holes, so that the fluorescence can enter the light measuring component 8, and then be transmitted through the first light measuring reflector 804 and the second light measuring reflector 805 and then be emitted from the light measuring component 8, and be received by the second light receiving component 13, or be reflected by the first light measuring reflector 804 and the second light measuring reflector 805 and then be emitted from the light measuring component 8 and be received by the third light receiving component 14 and the fourth light receiving component 15 respectively.

[0078] Further, the shape of the detection pool 16 is similar to a funnel shape, one end of which is wide and the other end is narrow, and the narrow end is connected with the pipeline 18, the sample to be detected enters from the wide end, and the sample to be detected is subjected to pressure from the surrounding wall due to the gradually reduced hole diameter of the path in the detection pool 16, so that the fluorescently labeled DNA or cells or particles suspended in the sample to be detected pass through the detection pool 16, and can generate fluorescence after being excited by the laser from the vertical direction.

[0079] Further, the shock pad 25 is a columnar structure, and the shock pad 25 is arranged through the thickness direction of the bottom plate 1, the axial section of the shock pad 25 is in the shape of an I-beam, the height of the recessed side of the I-beam is adapted to the thickness of the bottom plate 1, and the shock pad 25 is further provided with a through hole along the axial direction, so that the shock pad 25 can be fixed on the bottom plate 1 by a fastener. The rounded corners of the top corner of the bottom plate 1 not only have an aesthetic appearance, but also can avoid scratching other equipment or experimental personnel.

[0080] Further, the detection pool 16 and the pipeline 18 are both arranged through the bottom plate 1, rather than being closed and connected, so that there is a gap between the detection pool 16 and the pipeline 18 and the bottom plate 1, and the outlet 26 is a hole for the detection device to communicate with the outside, in order to prevent dust and the like from entering the inside of the detection device, thereby affecting the normal use of the components, therefore, dust-proof cotton is arranged between the detection pool 16 and the bottom plate 1, between the pipeline 18 and the bottom plate 1, and at the outlet 26.

[0081] Although the embodiments of the new utility model have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the new utility model, and those skilled in the art can make changes, modifications, replacements and modifications to the above embodiments within the scope of the new utility model.

Claims

1. A flow-through fluorescence detection device, characterized by, The detection device comprises at least a laser emitting component, a detection component, a filter component, a reflection component and a light receiving component arranged in the light path; The reflection component is arranged in the light path from the detection component to the light receiving component; The filter component is arranged in the light path from the laser emitting component to the detection component, or in the light path from the detection component to the reflection component; The laser emitting component comprises a first laser and a second laser; An optical path coupling component is arranged between the first laser, the second laser and the detection component; The optical path coupling component comprises a cover, a sealing gasket, an optical path coupling component housing, a first coupling filter, a second coupling filter, a third coupling filter, a first coupling reflection sheet, a second coupling reflection sheet, a third coupling reflection sheet and a convex lens sheet; The optical path coupling component housing comprises a trapezoidal frame and a first plate and a second plate arranged inside the trapezoidal frame, the trapezoidal frame comprises an upper base, a lower base, a first side and a second side, the first plate and the second plate are fixedly connected, the free end of the first plate is fixed at the connection between the first side and the upper base, and the free end of the second plate is fixed at the lower base; The first coupling filter and the second coupling filter are arranged on the lower base, the third coupling filter is arranged on the upper base, the first coupling reflection sheet is arranged on the first side, the second coupling reflection sheet is arranged on the second plate, the third coupling reflection sheet is arranged on the second side, and the convex lens sheet is arranged on the first plate; The first laser and the second laser are arranged opposite to the lower base of the trapezoidal frame, the laser emitted by the first laser and the second laser can enter the optical path coupling component through the filter arranged on the lower base of the trapezoidal frame, and is coupled through the reflection sheet and the convex lens sheet arranged inside the optical path coupling component and is emitted through the filter arranged on the upper base of the trapezoidal frame.

2. The flow-through fluorescence detection device of claim 1, wherein, The flow fluorescence detection device further comprises a data processing terminal for analyzing and processing the light signal received by the light receiving component, a bottom plate and an upper cover; The laser emitting component, the detection component, the filter component, the reflection component, the light receiving component and the data processing terminal are arranged on the bottom plate; The bottom plate is fixedly connected with the upper cover.

3. The flow fluorescence detection device according to claim 1, wherein The number of reflection components is multiple, and at least arranged in the light path from the detection component to the light receiving component; The reflection component comprises a reflection sheet, a reflection sheet fixing plate, a reflection sheet supporting plate, an elastic assembly, an elastic assembly fixing member and a fixing plate adjusting member; The reflection sheet is arranged on the reflection sheet fixing plate; The elastic assembly passes through the reflection sheet fixing plate and the reflection sheet supporting plate, and the two ends are fixed through the elastic assembly fixing member; The fixing plate adjusting member passes through the reflection sheet supporting plate and abuts against the reflection sheet fixing plate.

4. The flow fluorescence detection device according to claim 1, wherein The number of the light filtering components is multiple, and at least arranged on the light path from the laser emitting component to the detecting component and the light path from the detecting component to the reflecting component; The light filtering component comprises a lens fixing plate, a lens supporting plate, a lens, a lens fixing component, an elastic component, an elastic component fixing component and a fixing plate adjusting component; The lens is arranged on the lens fixing plate, and protrudes from both ends of the lens fixing plate, and is fixed by the lens fixing component; The elastic component passes through the lens fixing plate and the lens supporting plate, and is fixed by the elastic component fixing component at both ends, and one end of the lens is arranged in the through hole arranged on the lens supporting plate; The fixing plate adjusting component passes through the lens supporting plate and abuts against the lens fixing plate. 5.The flow fluorescence detection device according to claim 1, further comprising a light measuring component arranged on the light path between the reflecting component and the light receiving component; The light measuring component comprises a light measuring fixing plate, a double-plate supporting plate, a light measuring reflecting sheet, an elastic component, an elastic component fixing component, a fixing plate adjusting component and a light measuring component housing; The light measuring fixing plate is provided with a protruding structure, the protruding structure is provided with an inclined surface and a through hole penetrating the inclined surface, and the light measuring reflecting sheet is arranged on the inclined surface; The elastic component passes through the light measuring fixing plate and the double-plate supporting plate, and is fixed by the elastic component fixing component at both ends; The fixing plate adjusting component passes through the double-plate supporting plate and abuts against the light measuring fixing plate; The light measuring component housing is fixedly connected with the double-plate supporting plate. 6.The flow fluorescence detection device according to claim 5, wherein the number of the light receiving components is multiple, and at least arranged on the light path after the reflecting component, or on the light path after the light measuring component. 7.The flow fluorescence detection device according to claim 2, wherein the detecting component comprises a detecting pool, a pipeline and a pipeline support; The detecting pool and the pipeline support are both arranged on the bottom plate, one end of the pipeline passes through the pipeline support and is connected with the detecting pool, and the other end passes through the bottom plate and is connected with an external liquid path. 8.The flow fluorescence detection device according to claim 2, wherein the bottom plate is a rectangular plate, a shock-absorbing pad is arranged at a top corner position of the bottom plate or an edge of the bottom plate, and a top corner of the bottom plate is rounded. 9.The flow fluorescence detection device according to claim 7, further comprising a fan and a fan support; One end of the bottom plate is provided with the fan support, and the fan is installed on the fan support; The other end of the bottom plate is provided with a wire outlet. 10.The flow fluorescence detection device according to claim 9, further comprising a first laser fixing plate and a baffle plate provided with a through hole, the first laser is arranged on the bottom plate through the first laser fixing plate, the baffle plate is arranged in front of the first laser, and the laser emitted by the first laser can be transmitted through the through hole of the baffle plate. ​ ​ ​ ​ ​ ​ 11. The flow fluorescence detection device according to claim 10, characterized in that, a detection cell dustproof cotton is arranged between the detection cell and the bottom plate, a liquid path pipe dustproof cotton is arranged between the liquid path pipe and the bottom plate, and a wire outlet dustproof cotton is arranged at the wire outlet.

12. A flow-through fluorescence detection system characterized in that, The system comprises an automatic sample suction device, a power device, a control device, a box body, and the flow fluorescence detection device according to any one of claims 1-11; The flow fluorescence detection device, the automatic sample suction device, the power device, and the control device are all arranged on the box body; The control device is in communication connection with the power device; The power device is in electrical connection with the flow fluorescence detection device and the automatic sample suction device; The flow fluorescence detection device is in liquid connection with the automatic sample suction device.

Images