A sexual attractant for hibiscus mealybugs and its application
By developing sexual attractants for fusanthemum mealybugs, using active ingredient (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylenecyclobutyl) methyl ester and other components, the induce core was prepared, which solved the problem of difficult prevention and control of fusanthemum mealybugs, and achieved efficient lure and killing and predicting pest density, with significant economic and social benefits.
Patent Information
- Application Number
- CN202011163003.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2020-10-27
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2040-10-27
AI Technical Summary
As an important invasive pest in agriculture, the existing technology is difficult to effectively prevent and control the economic losses caused by the small individual, strong secrets and high reproductive power.
A sexual attraction agent for fusanthemum mealybugs, including active ingredient (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylenecyclobutyl) methyl ester, was developed, combined with antioxidants and solvents, and prepared into a lure core for trapping male insects.
This sexual attraction can efficiently lure and kill Fusang mealybugs, accurately predict the lower generation insect densities, effectively reduce the pest hazard and loss rate, and is simple, sensitive, efficient, strong specificity, environmentally friendly, and significant economic and social benefits.
Smart Images
Figure CN114467938B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of agricultural pest control, and in particular relates to a sex attractant for hibiscus mealybugs and an application thereof. Background Art
[0002] Phenacoccus solenopsis Tinsley, also known as cotton mealybug, belongs to the order Hemiptera, family Pseudococcidae, genus Phenacoccus Cockerel. Phenacoccus solenopsis Tinsley was originally native to North America and invaded my country in 2008. So far, it has invaded dozens of provinces and cities in my country, with distribution in both the north and south. It has strong adaptability and has become an important invasive pest in agriculture. Phenacoccus solenopsis Tinsley is polyphagous and has a wide range of hosts, including food crops, vegetable crops, ornamental flowers, garden plants and cash crops, causing serious economic losses every year. Due to the characteristics of small individuals, strong secretiveness, high reproductive capacity, overlapping generations, strong stress resistance and wax shell on the back of the body, Phenacoccus solenopsis Tinsley has obvious ecological advantages. Therefore, how to deal with Phenacoccus solenopsis Tinsley is a challenge we face.
[0003] Since the attracted insects are tiny, secretive, and have high reproductive capacity, chemical control is difficult to achieve the desired effect. Therefore, it is urgent to develop a "green control" technology for the hibiscus mealybug. Insect sex pheromones are trace chemicals secreted outside the body by special secretory organs of individuals of one sex of the same insect species, which can be accepted by the receptors of individuals of the opposite sex of the same species and cause certain behavioral responses in individuals of the opposite sex. Different insects have different sex pheromones to attract them, and the current control of the hibiscus mealybug mainly relies on chemical control. Therefore, the invention of a sex attractant that can specifically attract the hibiscus mealybug is of great significance for the green control of the hibiscus mealybug. Summary of the invention
[0004] In view of this, the object of the present invention is to provide a sex attractant that can monitor the population of Phenacoccus hibiscus and can effectively kill and control it.
[0005] In order to achieve the above-mentioned purpose of the invention, the present invention provides a sex attractant for Phenacoccus hibiscus powder, comprising the active ingredient (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester.
[0006] Preferably, the hibiscus powdery mildew attractant further comprises an antioxidant and / or a solvent.
[0007] Optionally, the antioxidant is one or more of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), tert-butylhydroquinone (TBHQ) and tocopherol.
[0008] Optionally, the solvent is one or more of n-hexane, chlorine dioxide and methanol.
[0009] Optionally, the mass ratio of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester to the antioxidant is 0.1 to 200:1, preferably 0.1 to 20:1.
[0010] Preferably, in the present invention, the concentration of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylidenecyclobutyl)methyl ester dissolved in the solvent is 0.1 to 100 μg / μL.
[0011] Another object of the present invention is to provide a hibiscus mealybug bait core, comprising a rubber stopper, wherein the rubber stopper contains the above-mentioned sex attractant. The bait core of the present invention can be stored at a low temperature of -20 to 5°C to reduce the volatilization and inactivation of the sex attractant in the bait core.
[0012] Preferably, the amount of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester added to the lure is 0.001 mg to 5 mg.
[0013] Preferably, the amount of antioxidant added to the lure is 0.001-10 mg.
[0014] Another object of the present invention is to provide a method for controlling hibiscus mealybugs, wherein the lure core is fixed on a sticky insect board and hung at a height of 0.8 to 2.5 m in the field at intervals of 4 to 10 m.
[0015] Compared with the prior art, the present invention has the following beneficial effects: the sex attractant of hibiscus mealybug provided by the present invention can be used for population monitoring and mass trapping and killing of hibiscus mealybugs, can accurately predict the occurrence density of the next generation of insects, and effectively reduce the harm and loss rate of hibiscus mealybugs. The sex attractant and its attractant core provided by the present invention are simple to prepare, sensitive, efficient, highly specific, do not produce antibodies, are harmless to humans and animals, are not afraid of wind, rain and sun, and have strong stress resistance; are environmentally friendly, have a long effective period, and are low in price; and have good economic benefits, social benefits, and ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 :Effects of different sex attractant dosage ratios on field trapping of male Phenacoccus hibiscus powdery mildew insects;
[0017] Figure 2 :Comparison of different color plates in trapping male Phenacoccus hibiscus;
[0018] Figure 3 : The use time of the lure core of the hibiscus mealybug sex attractant;
[0019] Figure 4: The degree of protection of different antioxidants on sex attractant core. DETAILED DESCRIPTION
[0020] The present invention provides a hibiscus mealybug sex attractant, which includes an active ingredient (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, and may also include an antioxidant and / or a solvent. The active ingredient is dissolved in n-hexane, chlorine dioxide or methanol solvent at a concentration of 0.1 to 100 μg / μL, and after dissolution, an antioxidant butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) propyl gallate (PG), tertiary butylhydroquinone (TBHQ) and tocopherol are added, and the hibiscus mealybug sex attractant is obtained after uniform mixing. The hibiscus mealybug sex attractant is dripped into a rubber plug to obtain the hibiscus mealybug attractant core of the present invention. The hibiscus mealybug attractant core of the present invention is fixed to the center of a blue sticky insect board, a yellow sticky insect board, a white sticky insect board and a black sticky insect board, and is hung at a height of 0.8 to 2.5 m in the field with an interval of 4 to 10 m to trap male hibiscus mealybugs.
[0021] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0022] Example 1
[0023] Weigh 10 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of n-hexane to make a solution with a concentration of 10 mg / mL, measure 1 μL, add 25 μL of the antioxidant butylated hydroxyanisole (BHA) and mix well, then drop it into the rubber stopper to make an attractant containing 10 μg of the active ingredient per attractant.
[0024] Example 2
[0025] Weigh 1 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of chlorine dioxide to make a solution with a concentration of 1 mg / mL, measure 1 μL, add 2 μL of the antioxidant butylated hydroxyanisole (BHA), mix well, and drop it into the rubber stopper to make an attractant containing 1 μg of the active ingredient per attractant.
[0026] Example 3
[0027] Weigh 1 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of methanol to make a solution with a concentration of 1 mg / mL, measure 50 μL, add 30 μL of the antioxidant butylated hydroxyanisole (BHA) and mix well, then drop it into the rubber stopper to make an attractant containing 50 μg of active ingredient per attractant.
[0028] Example 4
[0029] Weigh 1 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 10 mL of n-hexane to make a solution with a concentration of 0.1 mg / mL, measure 1 mL, add 50 μL of the antioxidant butylated hydroxyanisole (BHA) and mix well, then drop it into the rubber stopper to make an attractant containing 100 μg of the active ingredient per attractant.
[0030] Example 5
[0031] Weigh 10 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of n-hexane to make a solution with a concentration of 10 mg / mL, measure 0.1 mL, add 40 μL of antioxidant butyl hydroxyanisole (BHA) and mix well, then drop it into the rubber stopper to make an attractant containing 1 mg of active ingredient per attractant.
[0032] Example 6
[0033] Weigh 10 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of n-hexane to make a solution with a concentration of 10 mg / mL, measure 3 mL, add 50 μL of antioxidant butyl hydroxyanisole (BHA) and mix well, then drop it into the rubber stopper to make an attractant containing 3 mg of active ingredient per attractant.
[0034] Example 7
[0035] Weigh 10 mg of the sex pheromone of Phenacoccus hibiscus powder (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester, dissolve it in 1 mL of n-hexane to make a solution with a concentration of 10 mg / mL, measure 1, 5, 10, 20, 50 and 100 μL respectively, add 25 μL of the antioxidant butylated hydroxyanisole (BHA) to the measured solution and mix well, then drop it into a rubber stopper to make different doses, i.e. 10, 50, 100, 200, 500 and 1000 μg / lure cores, place the prepared sex attractant cores in aluminum foil bags and refrigerate at -20 to 5°C, with a shelf life of one month.
[0036] The field tests of the present invention were all carried out in Yangdu Village, Haining City, Jiaxing City, Zhejiang Province. There are a large number of hibiscus mealybugs in the area, which mainly harm crops such as eggplants, cucumbers and tomatoes. The above-made different doses of the attractant core are fixed to the center of the yellow sticky insect board (200*250mm) and hung in the place where the hibiscus mealybugs occur. The hanging height is 2m and the interval is 8m. After being placed for 3 days, the number of male hibiscus mealybugs in the sticky insect board is investigated, and statistical analysis is performed. Each treatment of this test is repeated 5 times, and the average value and error are calculated. The calculation results are detailed in the specification attached. Figure 1 The field trapping effect of different sex attractant dosage ratios on male Phenacoccus hibiscus mealybugs. The results of this example show that different doses of sex attractant have great differences in their ability to attract male Phenacoccus hibiscus mealybugs, among which 100 μg / lure core has the best effect, trapping 28 male insects.
[0037] Example 8
[0038] According to the method described in Example 7, a hibiscus mealybug bait containing 100 μg / bait of the hibiscus mealybug sex pheromone (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester was prepared, and the bait was fixed to the center of different color sticky insect boards (200*250mm), and hung in the place where the hibiscus mealybug occurred, with a hanging height of 2.5m and an interval of 10m. After being placed for 3 days, the number of male hibiscus mealybugs in the sticky insect board was investigated, and statistical analysis was performed. Each treatment was repeated 5 times, and the effect of different color sticky insect boards was analyzed by comparing the number of male hibiscus mealybugs trapped. The results showed that at a dosage of 100 μg / bait, the yellow sticky insect board had the best trapping effect, and the white sticky insect board had the worst effect.
[0039] Example 9
[0040] According to the method described in Example 7, a hibiscus mealybug bait containing 100 μg / bait of the hibiscus mealybug sex pheromone (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester was prepared. The bait was fixed to the center of a yellow board (200*250 mm) and hung at the site where the hibiscus mealybug occurred. The hanging height was 2 m and the interval was 10 m. The number of male hibiscus mealybugs in the sticky insect board was investigated after 1, 2, 3, 7, 14 and 30 days, and statistical analysis was performed. The results are detailed in the attached manual. Figure 3 The results of this embodiment show that in about half a month, as time goes by, the number of trapped male Phenacoccus hibiscus insects gradually increases. After half a month, the increase in the number of trapped male insects is not significant. Therefore, the use period of the lure core of the present invention is 2 weeks, and the lure core is replaced every two weeks to achieve a better trapping effect.
[0041] Example 10
[0042] According to the method described in Example 7, hibiscus mealybug baits containing 100 μg / bait of the sex pheromone (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester of the hibiscus mealybug and different antioxidants, namely butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) propyl gallate (PG), tertiary butylhydroquinone (TBHQ) and tocopherol, were prepared. The baits containing different antioxidants were fixed to the center of the yellow board (200*250mm) and hung at the site where the hibiscus mealybug occurred. The hanging height was 2.5m and the interval was 10m. After being placed for 3 days, the number of male hibiscus mealybugs in the sticky insect board was investigated and statistical analysis was performed. The analysis results are detailed in the attached manual. Figure 4 The degree of protection of different antioxidants on sex attractant cores.
[0043] The added antioxidant can protect the sex pheromone (R)-isoprenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester from being oxidized by environmental factors such as air and light, and can maintain the lasting biological activity of the sex pheromone. This example analyzes the interaction between different antioxidants and the sex attractant of Phenacoccus hibiscus. The results show that different antioxidants have the effect of protecting the sex pheromone of Phenacoccus hibiscus, among which butylated hydroxyanisole (BHA) has the best effect.
[0044] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A hibiscus mealybug bait core, characterized in that: The invention comprises a rubber stopper and a hibiscus mealybug sex attractant, wherein the hibiscus mealybug sex attractant comprises an active ingredient (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester; the hibiscus mealybug sex attractant also comprises an antioxidant and a solvent; the antioxidant is butylated hydroxyanisole, the solvent is n-hexane, the mass ratio of the (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester to the antioxidant is 0.1 to (200:1); the concentration of the (R)-isopentenyl acid-(2,2-dimethyl-3-isopropylcyclobutyl) methyl ester dissolved in the solvent is 100 μg / μL.
2. The hibiscus mealybug sex attractant core according to claim 1, characterized in that: The added amount of (R)-isopentenoic acid-(2,2-dimethyl-3-isopropylcyclobutyl)methyl ester in the lure is 0.001 mg to 5 mg.
3. The hibiscus mealybug sex attractant core according to claim 2, characterized in that: The amount of antioxidant added to the lure is 0.001-10 mg.
4. A method for controlling Phenacoccus hibiscus, characterized in that: The lure core described in claim 1 is fixed on an insect sticky board and hung in the field at a height of 0.8 to 2.5 m with an interval of 4 to 10 m.