A nucleic acid extraction device

Abstract

The present invention proposes a nucleic acid extraction device, including: a sample module, a reagent module, a pipetting module, a reaction module, a temperature control module and an extraction module, wherein the sample module contains a sample; the reagent module contains a plurality of reagents; the pipetting module is used to extract and transfer the liquid to be pipetted, and the liquid to be pipetted includes a sample and a reagent; the reaction module includes a reaction platform, and the reaction platform is used for reacting the sample and the reagent; the temperature control module is used to control the temperature of the reaction platform; and the extraction module is used to extract the sample after the reaction. After placing the sample in the nucleic acid extraction device provided by the present invention, the operations of sample pipetting, reagent addition, amplification and extraction can be automatically performed, and the contact between the test personnel and the sample, positive quality control products, etc., can be avoided as much as possible, and the possibility of infection of the test personnel during the test process is reduced. By performing automated nucleic acid extraction operations through the nucleic acid extraction device, less manpower is required for testing and high efficiency is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a nucleic acid extraction device. Background Art

[0002] How to quickly and effectively extract the effective nucleic acid of the test object during the pre-treatment process is the key environment for the detection of various drug-resistant bacteria, pathogenic bacteria, viruses, etc. The current pre-treatment method is mainly divided into several steps: sampling, nucleic acid extraction, and adding detection reagents. The entire operation process is manually recorded and matched one by one. The entire operation process is time-consuming and laborious, and there is a high possibility of sample contamination. In addition, it is easy for testers to come into contact with samples and get infected during manual operations.

[0003] When a large number of test objects need to be processed, the pre-processing operation will consume a lot of time and manpower, and the low efficiency will lead to a long test cycle. In addition, the existing technology is troublesome in information matching during the pre-processing process, which is easy to cause information errors and untraceable test results. Summary of the invention

[0004] In view of this, in order to overcome the above-mentioned defects of the prior art, the present invention proposes a nucleic acid extraction device for performing automated nucleic acid extraction.

[0005] The nucleic acid extraction device comprises:

[0006] A sample module, wherein the sample module comprises a first turntable, wherein a plurality of slots are provided on the first turntable, wherein the slots are used to accommodate sample adapters, wherein the sample adapters are used to accommodate sample tubes, wherein the sample tubes contain samples;

[0007] A reagent module, wherein the reagent module comprises a plurality of solvent chambers, wherein the plurality of solvent chambers contain a plurality of reagents;

[0008] A pipetting module, the pipetting module is used to extract and transfer the liquid to be pipetted, the liquid to be pipetted includes the sample and the reagent;

[0009] A reaction module, wherein the reaction module comprises a reaction platform, and the reaction platform is used for the sample and the reagent to react;

[0010] A temperature control module, wherein the temperature control module is used to control the temperature of the reaction platform;

[0011] An extraction module is used to extract the sample after the reaction.

[0012] The sample adapter comprises a main body and an upper cover, wherein the main body is used to accommodate the sample tube, and the upper cover is used to cover the sample tube;

[0013] The sample module also includes a cover opening mechanism and a tube removal mechanism, wherein the cover opening mechanism is used to open the upper cover of the sample adapter, and the tube removal mechanism is used to guide the sample adapter to move so that the sample adapter is removed from the card slot. When placing the sample, the tester only needs to touch the sealed sample tube, and the nucleic acid extraction device automatically opens the cover and removes the tube of the sample adapter to avoid contact between the tester and the sample.

[0014] The pipetting module includes a pipette tip, a pipette arm and a moving unit. The moving unit is connected to the pipette arm and is used to drive the pipette arm to move. The pipette arm is provided with a mounting mechanism for mounting the pipette tip. The pipette tip is used to extract and release the liquid to be pipetted.

[0015] The mobile unit includes a first mobile component and a second mobile component, wherein the first mobile component drives the pipetting arm to move on a horizontal plane, and the second mobile component drives the pipetting arm to move up and down. The pipetting module can move freely and accurately inside the nucleic acid extraction device to complete the pipetting operation.

[0016] The reaction module further includes a displacement component, which is used to drive the reaction platform to move to a first preset position in a direction close to the pipetting module or to move the reaction platform to a second preset position in a direction away from the pipetting module;

[0017] When the reaction platform moves to the first preset position, it is used for the pipetting module to transfer the liquid to be pipetted. When the reaction platform moves to the second preset position, the extraction module is located above the reaction platform.

[0018] The extraction module comprises an extraction unit and a lifting mechanism, wherein the extraction unit is connected to the lifting mechanism;

[0019] The extraction unit is used to extract the sample after the reaction, and the lifting mechanism is used to drive the extraction unit to rise or fall.

[0020] In some embodiments, the nucleic acid is extracted by magnetic bead method, the nucleic acid extraction device further comprises a magnetic bead tube and a magnetic suction system, and the extraction module comprises a magnetic rod;

[0021] The magnetic bead tube contains magnetic beads, the magnetic suction system is used to absorb the magnetic beads from the magnetic bead tube and add the magnetic beads to the reaction platform, and the magnetic rod is used to absorb the magnetic beads in the reaction platform.

[0022] Furthermore, the nucleic acid extraction device also includes a refrigeration module, in which a stabilizer is arranged.

[0023] In some embodiments, the nucleic acid extraction device further includes a quality control product module and a control module, wherein the quality control product module contains quality control standards, and the control module includes multiple culture units, and the multiple culture units are used to accommodate the reacted samples and the quality control standards.

[0024] The nucleic acid extraction device further comprises a scanning module, which is used to obtain information of the sample. The scanning module is used to obtain information of the sample currently being extracted, so that the information of the sample is accurate and the test result is traceable.

[0025] In summary, the nucleic acid extraction device of the present invention has the following beneficial effects: the nucleic acid extraction operation is performed by machine automation, and the test personnel only need to touch the sealed sample tube and the consumables required in the detection process, thereby avoiding the contact between the test personnel and the samples, positive quality control products, etc. as much as possible, and reducing the possibility of infection of the test personnel during the detection process.

[0026] Automated nucleic acid extraction operations require less manpower and are more efficient for testing.

[0027] The nucleic acid extraction device records and matches the sample information during the nucleic acid extraction process, which can ensure that the information of the samples matches each other, so that the information of the samples after nucleic acid extraction is accurate and the test results are traceable. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0029] Figure 1 It is a schematic diagram of the overall structure of the nucleic acid extraction device of the present invention;

[0030] Figure 2 is a schematic diagram of the overall structure of the nucleic acid extraction device of the present invention from another angle;

[0031] Figure 3 It is a partial structural schematic diagram of the nucleic acid extraction device of the present invention;

[0032] Figure 4 It is a schematic structural diagram of a reagent module of a nucleic acid extraction device of the present invention;

[0033] Figure 5 It is a schematic diagram of the structure of the extraction module of the nucleic acid extraction device of the present invention;

[0034] Figure 6It is a schematic diagram of the structure of a sample module of the nucleic acid extraction device of the present invention;

[0035] Figure 7 It is a schematic diagram of the local structure of the sample module;

[0036] Figure 8 It is a structural schematic diagram of the cap removal block;

[0037] Fig. 9 It is a structural schematic diagram of the tube removal block;

[0038] Fig.10 A schematic diagram of the structure of a sample adapter for accommodating a sample tube;

[0039] Fig.11 It is a schematic diagram of the structure of the sample module and the scanning module being assembled together;

[0040] Fig.12 It is a structural schematic diagram of a multi-station pipe sleeve warehouse;

[0041] Fig.13 A schematic diagram of another structure of a multi-station pipe housing;

[0042] Fig.14 A schematic diagram of a tube sleeve structure for accommodating reagent tubes.

[0043] Reference numerals:

[0044] 1-sample module; 11-first turntable; 12-card slot; 13-sample adapter; 14-sample tube; 15-main body; 16-upper cover; 17-cap removal block; 171-first inclined plane; 18-tube removal block; 181-second inclined plane; 19-first drive mechanism; 2-reagent module; 21-solvent tank; 22-overflow tank; 3-pipette module; 31-pipette tip; 32-pipette arm; 33-tip box unit; 4-reaction module; 41-reaction platform; 42-displacement assembly ;5-extraction module;51-extraction unit;52-lifting mechanism;53-magnetic bead tube;6-scanning module;7-refrigeration module;71-warehouse body;72-cover body;73-second turntable;74-accommodating groove;740-tube sleeve structure;75-second driving mechanism;76-tube sleeve body;77-tube cover;78-screwing cover mechanism;79-reagent tube;8-quality control product module;81-stop structure;82-through hole;83-guide structure;9-control module;10-operation panel. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] The invention provides a nucleic acid extraction device for performing automated nucleic acid extraction operations.

[0047] Example 1

[0048] See also Figure 1-Figure 3 The nucleic acid extraction device includes a sample module 1, a reagent module 2, a pipetting module 3, a reaction module 4, a temperature control module and an extraction module 5. The sample module 1 includes a first turntable 11, and a plurality of slots 12 are provided on the first turntable 11. The slots 12 are used to accommodate a sample adapter 13, and the sample adapter 13 is used to accommodate a sample tube 14, and the sample tube 14 contains a sample. The reagent module 2 includes a plurality of solvent compartments, and the plurality of solvent compartments contain a plurality of reagents. The pipetting module 3 is used to extract and transfer the liquid to be pipetted, and the liquid to be pipetted includes a sample and a reagent. The reaction module 4 includes a reaction platform 41, and the reaction platform 41 is used for reacting the sample and the reagent. The temperature control module is used to control the temperature of the reaction platform 41. The extraction module 5 is used to extract the sample after the reaction.

[0049] When the nucleic acid extraction device provided by the present invention is used to perform a nucleic acid extraction operation, the sample tube 14 containing the sample is sealed by the sample adapter 13 and then placed on the sample module 1. The sample is extracted by the pipetting module 3, and then the sample is transferred to the reaction platform 41. Then, the reagent is extracted from the reagent module 2 by the pipetting module 3 and transferred to the reaction platform 41, and the sample and the reagent react on the reaction platform 41. The temperature control module controls the temperature of the reaction platform 41 when the sample and the reagent are amplified in the reaction platform 41, so that the temperature change occurring in the reaction platform 41 meets the temperature change requirement for nucleic acid amplification. After the sample and the reagent complete the reaction on the reaction platform 41, the sample after the reaction is extracted by the extraction module 5, and the sample after the reaction extracted by the extraction module 5 is used for nucleic acid detection.

[0050] For details, see the attached manual. Figure 4In this embodiment, the multiple solvent tanks 21 of the reagent module 2 are connected to a solvent storage device. The capacity of the solvent storage device is much larger than that of the solvent tank 21. A pump is provided between the solvent tank 21 and the solvent storage device. A first liquid level detection device and a second liquid level detection device are provided in the solvent tank 21. The first liquid level detection device is provided at the bottom of the solvent tank 21 to detect whether the solvent tank 21 is empty. The second liquid level detection device is provided at the top of the solvent tank 21 to detect whether the solvent tank 21 is full. When the first liquid level detection device detects that the solvent tank 21 is empty, the reagent in the solvent storage device is pumped into the solvent tank 21 by the pump until the second liquid level detection device detects that the solvent tank 21 is full and stops. An overflow trough 22 is provided on the side of the solvent tank 21. When the reagent liquid level in the solvent tank 21 is higher than a preset height, it will flow into the overflow trough 22 to avoid mutual contamination of reagents in different solvent tanks 21.

[0051] The pipetting module 3 includes a pipette tip 31, a pipette arm 32 and a moving unit, wherein the moving unit is connected to the pipette arm 32 and is used to drive the pipette arm 32 to move, and a mounting structure is provided on the pipette arm 32 for mounting the pipette tip 31, and the pipette tip 31 is used to extract and release the liquid to be pipetted. Further, the pipetting module 3 also includes a tip box unit 33 and a tip removal unit, wherein the tip box unit 33 is used to accommodate the pipette tip 31.

[0052] Before extracting samples through the pipetting module 3 each time, the mobile unit controls the pipetting arm 32 to move to the tip box unit 33, and the pipetting tip 31 is installed on the pipetting arm 32. Then, the pipetting arm 32 is driven to rotate to the sample module 1 to extract the sample. After the extracted sample is transferred to the reaction platform 41, the pipetting arm 32 is driven to rotate to the tip removal unit to remove the pipetting tip 31. Before extracting the sample next time, the pipetting arm 32 is driven again to move to the tip box unit 33 to install a new pipetting tip 31, so as to avoid sample contamination.

[0053] Before extracting reagents through the pipetting module 3 each time, the mobile unit controls the pipetting arm 32 to move to the tip box unit 33, installs the pipetting tip 31 on the pipetting arm 32, and then drives the pipetting arm 32 to rotate to the reagent module 2 to extract the reagents. After the extracted reagents are transferred to the reaction platform 41, the pipetting arm 32 is driven to rotate to the tip removal unit to remove the pipetting tip 31. Before extracting the reagents next time, the pipetting arm 32 is driven again to move to the tip box unit 33 to install a new pipetting tip 31 to avoid reagent contamination.

[0054] The mobile unit includes a first mobile component and a second mobile component. The first mobile component drives the pipetting arm 32 to move on the horizontal plane so that the free end of the pipetting arm 32 moves above different modules. The second mobile component drives the pipetting gun head 31 to move up and down so that the pipetting arm 32 extends into different modules for operation.

[0055] For example, a mounting structure is provided on the free end of the pipetting arm 32, the first moving component drives the pipetting arm 32 to move so that the free end moves to the top of the gun tip box unit 33, the second moving component drives the pipetting arm 32 to move downward to install the pipetting gun tip 31, and after the installation is completed, the second moving component drives the pipetting arm 32 to move upward. Next, the first moving component drives the pipetting arm 32 to move so that the free end moves to the top of the sample module 1, and then the second moving component drives the pipetting arm 32 to move downward to extract the sample, and after the sample is extracted, the second moving component drives the pipetting arm 32 to move upward. Next, the first moving component drives the pipetting arm 32 to move so that the free end moves to the top of the reaction platform 41, and then the second moving component drives the pipetting arm 32 to move downward to release the sample extracted from the sample module 1 into the reaction platform 41, and after the sample is released, the second moving component drives the pipetting arm 32 to move upward. Then the first moving component drives the pipetting arm 32 to move so that the free end moves to the gun tip removal unit to remove the pipetting gun tip 31. According to the above operation flow, a sample pipetting operation is completed. Similarly, reagent pipetting operations can be realized. Circularly executing the above operation flow can complete multiple sample pipetting operations and multiple reagent pipetting operations.

[0056] The reaction platform 41 is provided with a plurality of reaction units. Each sample pipetting operation transfers different samples to different reaction units, and one reaction unit contains one sample. When performing the reagent pipetting operation, the reagent used to react with the sample is transferred to the containing unit containing the corresponding sample.

[0057] After the transfer of the sample and the reagent is completed, the sample and the reagent react in the reaction platform 41. During the reaction of the sample and the reagent, the temperature control module controls the temperature of the reaction platform 41 so that the temperature change of the reaction platform 41 during the reaction of the sample and the reagent meets the temperature change requirements for nucleic acid amplification. Specifically, the temperature control module may include a heating plate, which is arranged below the reaction platform 41, and the temperature generated by the heating plate can be transmitted to the reaction platform 41. During the reaction of a sample and a reagent, the heating plate can be controlled to heat to a preset temperature, and then the heating plate is turned off after a preset time, so that the reaction platform 41 is naturally cooled down, and the amplification reaction of the sample and the reagent is completed after a preset reaction time.

[0058] The reaction module 4 also includes a displacement component 42, which is used to drive the reaction platform 41 to move to a first preset position in a direction close to the pipetting module 3 or to drive the reaction platform 41 to move to a second preset position in a direction away from the pipetting module 3. When the reaction platform 41 moves to the first preset position, it is used for the pipetting module 3 to transfer the liquid to be pipetted. When the reaction platform 41 moves to the second preset position, the extraction module 5 is located above the reaction platform 41. The extraction module 5 includes an extraction unit 51 and a lifting mechanism 52, and the extraction unit 51 is connected to the lifting mechanism 52. The extraction unit 51 is used to extract the sample after the reaction, and the lifting mechanism 52 is used to drive the extraction unit 51 to rise or fall.

[0059] After the amplification reaction of the sample and the reagent is completed, the displacement assembly 42 drives the reaction platform 41 to move to the second preset position. The lifting mechanism 52 drives the extraction unit 51 to descend, and the extraction unit 51 extracts the sample after the reaction. After the extraction is completed, the lifting mechanism 52 drives the extraction unit 51 to rise.

[0060] In the present embodiment, the magnetic bead method is used to extract nucleic acid, so the nucleic acid extraction device in the present embodiment also includes a magnetic bead tube 53 and a magnetic suction system, and the extraction unit 51 is a magnetic rod. The magnetic bead tube 53 contains magnetic beads, the magnetic suction system is used to absorb magnetic beads from the magnetic bead tube 53 and add magnetic beads to the reaction platform 41, and the magnetic rod is used to absorb the magnetic beads in the reaction platform 41. The specific process is: after the amplification reaction of the sample and the reagent is completed, the magnetic suction system absorbs the magnetic beads from the magnetic bead tube 53 and adds the absorbed magnetic beads to the reaction platform 41. The magnetic beads are combined with the nucleic acid molecules in the reaction platform 41. After a preset time, the lifting mechanism 52 drives the magnetic rod to descend, and the magnetic rod absorbs the magnetic beads combined with the nucleic acid molecules to complete the nucleic acid extraction.

[0061] In this embodiment, a 96-well plate is arranged on the reaction platform 41 to form a plurality of reaction units. After the extraction module 5 completes the nucleic acid extraction, the process further includes: removing the original 96-well plate on the reaction platform 41 and adding a new 96-well plate to the reaction platform 41 .

[0062] Example 2

[0063] Based on the embodiment 1, this embodiment 1 provides a specific example of automatically opening the cover and removing the tube of the sample adapter 13.

[0064] See also Figure 5-Figure 10 In this embodiment, the sample adapter 13 includes a main body 15 and an upper cover 16, the main body 15 is used to accommodate the sample tube 14, and the upper cover 16 is used to cover the sample tube 14. The sample module 1 includes a cover opening mechanism and a tube removal mechanism, the cover opening mechanism is used to open the upper cover 16 of the sample adapter 13, and the tube removal mechanism is used to guide the sample adapter 13 to move so that the sample adapter 13 is removed from the card slot 12.

[0065] In this embodiment, the slot 12 is disposed at the outer edge of the first turntable 11, and one end of the slot 12 is open. A protrusion structure for engaging the first turntable 11 is disposed on the side wall of the sample adapter 13, so that the sample adapter 13 is supported on the first turntable 11. The sample adapter 13 is installed on the first turntable 11 by sliding the sample adapter 13 from the opening of the slot 12 into the slot 12, and the sample adapter 13 can be removed from the first turntable 11 by sliding the sample adapter 13 toward the opening direction of the slot 12.

[0066] The cover opening mechanism includes a cap removal block 17, which is arranged above the first turntable 11. A bump is arranged on the upper cover 16 of the sample adapter 13, and the bump can easily push the upper cover 16 to move. The height of the lower end surface of the cap removal block 17 is lower than the height of the upper end surface of the bump. The second driving mechanism 75 drives the first turntable 11 to rotate. When the sample adapter 13 rotates to a position corresponding to the cap removal block 17, the bump on the upper cover 16 of the sample adapter 13 contacts the cap removal block 17.

[0067] A first inclined surface 171 is formed on the side of the cap removal block 17, and the first inclined surface 171 is used to guide the movement of the upper cover 16. The sample adapter 13 moves to the cover opening mechanism to complete the cover opening, and then stops moving after moving to the preset area, that is, the second driving mechanism 75 stops moving after moving to the tube mouth of the sample tube 14 is not blocked, so that the sample tube 14 after the cover is opened stays in the preset area for sample extraction.

[0068] See the instruction manual Fig.11 Preferably, the nucleic acid extraction device further includes a scanning module 6, which is used to obtain sample information. Specifically, an identification code containing information about the sample in the sample tube 14 is provided on the sample adapter 13 or the sample tube 14, and the scanning mechanism is provided corresponding to the preset area, and is used to scan the identification code to obtain sample information when the sample adapter 13 stays in the preset area for sample extraction, so that the sampling device can ensure that the information of the sample matches the sample during the process of nucleic acid extraction from the sample, so that the information of the sample after nucleic acid extraction is accurate and the test result is traceable.

[0069] The tube-ejecting mechanism is used to guide the sample adapter 13 to move after the operation of extracting the sample in the sample tube 14 is completed, so that the sample adapter 13 is moved out of the card slot 12. In this embodiment, the first rotating disk 11 is connected to the first driving mechanism 19 to drive the first rotating disk 11 to rotate, and the first driving mechanism 19 drives the first rotating disk 11 to rotate clockwise, and the tube-ejecting mechanism is arranged on the left side of the cover-opening mechanism, so that the sample adapter 13 first passes through the cover-opening mechanism to open the cover during the movement, and then moves to the tube-ejecting mechanism to remove the tube.

[0070] The tube removal mechanism includes a tube removal block 18, and a second inclined surface 181 is formed on one side of the tube removal block 18 facing the cover opening mechanism. The second inclined surface 181 is used to guide the main body 15 of the sample adapter 13 to move toward the outer edge of the first turntable 11. The second driving mechanism 75 drives the first turntable 11 to rotate. When the main body 15 of the sample adapter 13 contacts the second inclined surface 181, the main body 15 of the sample adapter 13 moves along the second inclined surface 181 toward the outer edge of the first turntable 11 under the guidance of the second inclined surface 181, so as to move the main body 15 of the sample adapter 13 out of the card slot 12.

[0071] Furthermore, the sampling device may also include a slide mechanism, the slide mechanism includes a slide part and a collection part, and the slide mechanism is arranged below the cover opening mechanism and / or the tube removal mechanism. One end of the slide part may be connected to the bottom of the cover opening mechanism and / or the tube removal mechanism, and the other end of the slide part may be connected to the collection part, and the upper cover 16 of the sample adapter 13 after the cover is opened and / or the main body 15 of the sample adapter 13 after the tube is removed and the sample tube 14 contained in the main body 15 may slide out through the slide part to the collection part for collection for easy processing.

[0072] As a preferred solution, the slide mechanism includes a first slide, a second slide, a first collecting tank and a second collecting tank. The first slide is arranged below the cover opening mechanism, one end of the first slide is connected to the cover opening mechanism, and the other end of the first slide is connected to the first collecting tank. After the cover opening mechanism completes the cover opening, the upper cover 16 of the sample adapter 13 slides out to the first collecting tank through the first slide, and the discarded upper cover 16 can be automatically collected for easy processing. The second slide is arranged below the tube removal mechanism, one end of the second slide is connected to the tube removal mechanism, and the other end of the second slide is connected to the second collecting tank. After the tube removal mechanism completes the tube removal, the main body 15 of the sample adapter 13 and the sample tube 14 contained in the main body 15 slide out to the second collecting tank through the second slide, and the discarded main body 15 and sample tube 14 of the sample adapter 13 can be automatically collected for easy processing.

[0073] Other contents that are the same as those in Example 1 will not be repeated here.

[0074] Example 3

[0075] On the basis of Example 1, the nucleic acid extraction device provided in this embodiment also has a quality control mode, which can realize the quality control function.

[0076] Specifically, the nucleic acid extraction device also includes a refrigeration module 7, a quality control product module 8 and a control module 9. The refrigeration module 7 is provided with a stabilizer, the quality control product module 8 contains quality control standards, and the control module 9 includes multiple culture units, which are used to accommodate samples after reaction and quality control standards.

[0077] In the quality control mode, the nucleic acid extraction device first adds the stabilizer in the cold storage module 7 to the culture unit of the control module 9 through the pipetting module 3, and then adds the quality control standard in the quality control module 8 and the sample after the amplification reaction in the reaction module 4 to different culture units in the control module 9. The quality control standard and the sample are cultured under the same culture conditions in the control module 9 for comparison.

[0078] In some embodiments, eight tubes are connected in the control module 9 to form a plurality of culture units.

[0079] The setting of quality control standards needs to avoid contamination of samples, reagents, etc. in other structures, and when setting multiple quality control standards, it is necessary to avoid mutual contamination of different quality control products. The stabilizer needs to be refrigerated, which makes the storage requirements of the stabilizer different from the storage requirements of samples, reagents, etc. in other structures in the nucleic acid extraction device. The setting requirements of the quality control product module 8 and the refrigeration module 7 as mentioned above are the main problems in adding the quality control product module 8 and the refrigeration module 7 to the nucleic acid extraction device to realize the quality control function. In order to solve the above problems, the present embodiment provides a multi-station tube-in-cell structure.

[0080] See also Figure 12-14 The multi-station tube-in-tube warehouse comprises a warehouse body 71 and a cover body 72. The cover body 72 covers the opening of the warehouse body 71. A second turntable 73 is arranged in the warehouse body 71. A receiving groove 74 is arranged on the second turntable 73. The receiving groove 74 is used to receive the tube-in-tube structure 740. The second turntable 73 is connected to a second driving mechanism 75. The second driving mechanism 75 is used to drive the second turntable 73 to rotate. The tube-in-tube structure 740 comprises a tube-in-tube body 76, a tube cover 77 and a screw-on mechanism 78. The tube-in-tube body 76 is used to receive a reagent tube 79. One end of the screw-on mechanism 78 is rotatably connected to the main body 15. The other end of the screw-on mechanism 78 is connected to the tube cover 77. The tube cover 77 is used to cover the reagent tube 79. Each reagent tube 79 arranged on the multi-station tube-in-tube warehouse can be individually covered and sealed to avoid affecting other reagent tubes 79 when operating one reagent tube 79. When the multi-station tube-in-tube chamber is arranged in the nucleic acid extraction device, only the through hole 82 can enter the multi-station tube-in-tube chamber to perform extraction operations, and the rest of the structure is a closed structure.

[0081] A stopper structure 81 is provided on the inner wall of the bin body 71, and the stopper structure 81 is used to guide the screw cap mechanism 78 to open the tube cover 77. A through hole 82 is provided on the cover body 72, and the through hole 82 corresponds to the position of the stopper structure 81. When the tube cover 77 of the tube sleeve structure 740 is opened, the tube mouth of the reagent tube 79 is exposed from the through hole 82. The pipetting module 3 can extract the quality control standard, stabilizer, etc. in the reagent tube 79 whose tube cover 77 is in the open state through the through hole 82, and the remaining reagent tubes 79 are in the covered state, which can avoid affecting the remaining reagent tubes 79 during the operation.

[0082] In order to ensure that the tube sleeve structure 740 tightly covers the reagent tube 79, a guiding structure 83 is provided on the inner wall of the chamber body 71, and the guiding structure 83 is used to guide the screw cap mechanism 78 to cover the tube cover 77 so that the tube cover 77 tightly covers the tube mouth of the reagent tube 79.

[0083] Specifically, the stopper structure 81 and the guide structure 83 are both arranged along the circumference of the inner wall of the bin body 71, and the stopper structure 81 is provided with an arc surface, which is used to guide the rotation of the capping mechanism 78 of the tube sleeve structure 740 so as to open the tube cover 77. In the horizontal direction, the guide structure 83 is provided with inclined surfaces at both ends facing the stopper structure 81, which are used to guide the rotation of the capping mechanism 78 so as to cover the tube cover 77. The lower end surface of the capping mechanism 78 of the tube sleeve structure 740 abuts the guide structure 83, and the guide structure 83 makes the tube cover 77 of the tube sleeve structure 740 cover the reagent tube 79. When the second driving mechanism 75 drives the second rotating disk 73 to rotate so that the tube sleeve structure 740 rotates toward the position where the stopper structure 81 is provided, the lower end surface of the capping mechanism 78 is separated from the guide structure 83, and the upper end surface of the capping mechanism 78 abuts the arc surface of the stopper structure 81 and moves along the arc surface of the stopper structure 81 during the rotation process, so that the capping mechanism 78 rotates to open the tube cover 77. When the capping mechanism 78 moves to the lowest point of the stop structure 81, the capping mechanism 78 rotates to the maximum opening angle. The second turntable 73 continues to rotate, and the capping mechanism 78 gradually rotates in the closing direction. When the lower end surface of the rotating mechanism contacts the inclined surface of the guide structure 83, it moves along the inclined surface of the guide structure 83, so that the capping mechanism 78 rotates to the tube cover 77 to cover the reagent tube 79.

[0084] In this embodiment, the quality control product module 8 includes the above-mentioned multi-station tube housing, and the reagent tubes 79 in the multi-station tube housing are used to accommodate quality control standards. Placing the quality control standards separately in the multi-station tube housing can prevent the quality control standards from contaminating samples, reagents, etc. in other structures, and can prevent the quality control products placed in different reagent tubes 79 from contaminating each other. Optionally, the quality control product module 8 can be set according to actual needs, and can include a positive quality control product unit or a negative quality control product unit, or can include a positive quality control product unit and a negative quality control product unit.

[0085] The refrigeration module 7 includes the above-mentioned multi-station tube-sleeve warehouse and a refrigeration component, the refrigeration component includes a refrigerator and a refrigeration switch, the refrigerator is arranged in the warehouse body 71, the refrigerator is electrically connected to the refrigeration switch, and the refrigeration switch is used to control the opening and closing of the refrigerator. The reagent tube 79 in the multi-station tube-sleeve warehouse of the refrigeration module 7 is used to accommodate the stabilizer. A refrigeration environment can be formed in the refrigeration module 7, which meets the storage requirements of the stabilizer and will not affect the storage environment of structures such as the sample module 1 and the reagent module 2.

[0086] Other contents that are the same as those in Example 1 will not be repeated here.

[0087] Example 4

[0088] The nucleic acid extraction device provided in this embodiment includes a control module, which is connected to a sample module 1, a reagent module 2, a pipetting module 3, a reaction module 4, a temperature control module, an extraction module 5, a refrigeration module 7, a quality control product module 8 and a control module 9. The functions implemented by the control module include: obtaining the status of each module connected to it, setting the operation process of each module connected to it, storing the number of consumables such as pipette tips 31, 96-well plates, and eight-tube strips set in the nucleic acid extraction device, and recording the changes in the quantity of the above consumables according to the operation process of the nucleic acid extraction device.

[0089] The control module is provided with an operation panel 10, which is used to display the status of each module in the nucleic acid extraction device identified by the control module, including the operating status of each module, the remaining amount of consumables set in each module, etc. The test personnel can obtain relevant information of each module in the nucleic acid extraction device through the operation panel 10, so as to replenish the consumables in time.

[0090] The testing personnel can also use the operation panel 10 to set the operation sequence of each module of the nucleic acid extraction device, set data such as the reaction time of the reaction platform 41, the temperature change adjustment of the temperature control module, etc. The operation of each module of the nucleic acid extraction device can be carried out according to the preset process, or according to the customized settings of the testing personnel to form a new operation process.

[0091] The control module can also store multiple operation processes, such as the process executed in the conventional mode for conventional nucleic acid extraction operations, the process executed in the quality control mode of the nucleic acid extraction device, etc. The stored process information can be obtained and applied through the control panel.

[0092] In some embodiments, a sterilization module is further provided in the nucleic acid extraction device, and the sterilization module is connected to the control module. For example, the sterilization module includes an ultraviolet lamp, and the control module is used to control the opening and closing of the ultraviolet lamp. Preferably, the opening time and closing time of the sterilization module can be customized through the control module.

[0093] In some embodiments, the nucleic acid extraction device further comprises a fan, which is used to achieve directional and stable ventilation of the internal environment of the nucleic acid extraction device. For example, the fan is arranged to extract the air inside the nucleic acid extraction device to form a negative pressure environment inside the nucleic acid extraction device.

[0094] In summary, the nucleic acid extraction device provided by the present invention can automatically perform nucleic acid extraction operations, requires less manpower for detection, is highly efficient, and can perform rapid and accurate large-scale nucleic acid extraction operations.

[0095] Through the automated nucleic acid extraction operation, the test personnel only need to touch the sealed sample tube and the consumables required in the test process, which can avoid the contact between the test personnel and the samples, positive quality control products, etc. as much as possible, and reduce the possibility of infection during the test process. Furthermore, it reduces the contact between samples, positive quality control products, etc. and the environment to avoid pollution to the environment.

[0096] The nucleic acid extraction device records and matches the sample information during the nucleic acid extraction process, which can ensure that the information of the samples matches each other, so that the information of the samples after nucleic acid extraction is accurate and the test results are traceable.

[0097] In addition, the nucleic acid extraction device of the present invention can also realize the quality control function, can detect the performance of the nucleic acid extraction equipment, and improve and thereby ensure the accuracy of the information obtained by the nucleic acid extraction equipment.

[0098] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. In addition to the above embodiments, there may also be different variations. The technical features of the above embodiments may be combined with each other. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A nucleic acid extraction device, characterized in that: include: A sample module, wherein the sample module comprises a first turntable, wherein a plurality of slots are provided on the first turntable, wherein the slots are used to accommodate sample adapters, wherein the sample adapters are used to accommodate sample tubes, wherein the sample tubes contain samples; A reagent module, wherein the reagent module comprises a plurality of solvent tanks, wherein the plurality of solvent tanks contain a plurality of reagents, wherein a first liquid level detection device and a second liquid level detection device are arranged in the solvent tank, wherein the first liquid level detection device is arranged at the bottom of the solvent tank, and the second liquid level detection device is arranged at the top of the solvent tank, and an overflow tank is arranged on the side of the solvent tank; A pipetting module, the pipetting module is used to extract and transfer the liquid to be pipetted, the liquid to be pipetted includes the sample and the reagent; A reaction module, wherein the reaction module comprises a reaction platform, and the reaction platform is used for the sample and the reagent to react; A temperature control module, wherein the temperature control module is used to control the temperature of the reaction platform; An extraction module, wherein the extraction module is used to extract the sample after the reaction; The sample adapter comprises a main body and an upper cover, wherein the main body is used to accommodate the sample tube, and the upper cover is used to cover the sample tube; The sample module further comprises a cover opening mechanism and a tube removal mechanism, wherein the cover opening mechanism is used to open the upper cover of the sample adapter, and the tube removal mechanism is used to guide the movement of the sample adapter so that the sample adapter is moved out of the card slot; It also includes a quality control product module and a control module, wherein the quality control product module contains quality control standards, and the control module includes multiple culture units, and the multiple culture units are used to accommodate the reacted samples and the quality control standards; the pipetting module includes a pipetting arm and a moving unit, and the moving unit is connected to the pipetting arm to drive the pipetting arm to move; the moving unit includes a first moving component and a second moving component, the first moving component drives the pipetting arm to move on a horizontal plane, and the second moving component drives the pipetting arm to move up and down.

2. The nucleic acid extraction device according to claim 1, characterized in that: The pipetting module also includes a pipette tip. The pipetting arm is provided with a mounting mechanism for mounting the pipette tip. The pipette tip is used to extract and release the liquid to be pipetted.

3. The nucleic acid extraction device according to claim 1, characterized in that: The reaction module further includes a displacement component, which is used to drive the reaction platform to move to a first preset position in a direction close to the pipetting module or to move the reaction platform to a second preset position in a direction away from the pipetting module; When the reaction platform moves to the first preset position, it is used for the pipetting module to transfer the liquid to be pipetted. When the reaction platform moves to the second preset position, the extraction module is located above the reaction platform.

4. The nucleic acid extraction device according to claim 1, characterized in that: The extraction module comprises an extraction unit and a lifting mechanism, wherein the extraction unit is connected to the lifting mechanism; The extraction unit is used to extract the sample after the reaction, and the lifting mechanism is used to drive the extraction unit to rise or fall.

5. The nucleic acid extraction device according to claim 1, characterized in that: It also includes a magnetic bead tube and a magnetic suction system, and the extraction module includes a magnetic rod; The magnetic bead tube contains magnetic beads, the magnetic suction system is used to absorb the magnetic beads from the magnetic bead tube and add the magnetic beads to the reaction platform, and the magnetic rod is used to absorb the magnetic beads in the reaction platform.

6. The nucleic acid extraction device according to claim 1, characterized in that: It also includes a refrigeration module, in which a stabilizer is arranged.

7. The nucleic acid extraction device according to claim 1, characterized in that: It also includes a scanning module, which is used to obtain information about the sample.

Images