A flow cytometer and its working method

Abstract

A flow cytometer and a working method thereof, relating to the field of medical detection technology, comprises: a host, a liquid storage system and an analysis system. After a sample is injected, the sample is analyzed by an optical system, and at the same time, a first flow meter and a second flow meter are used to respectively obtain the flow rate of the sample output from an infusion tube to a sample output tube and the flow rate of the sheath liquid output from a sheath liquid pump to a flow chamber. By analyzing the information obtained by the optical system, the running speeds of the quantitative pump and the sheath liquid pump are adjusted, so that the flow cytometer can maximize the detection speed while maintaining the detection accuracy, thereby solving the problem that the detection accuracy cannot be maintained when the detection speed is increased in the current flow cytometer.

Description

Technical Field

[0001] The present invention relates to the technical field of medical detection, and in particular to a flow cytometer and a working method thereof. Background Art

[0002] Flow cytometers can be used to analyze sample fluids containing samples or particles and identify the characteristics of the samples or particles contained in the fluids. These samples or particles can be biological samples or physical samples collected for analysis and / or separation. The samples are mixed with sheath fluid to transport the samples or particles through the flow cytometer. The particles can include biological samples, calibration beads, physical sample particles, or other particles of interest. Specific information about the samples can be obtained by analyzing them through an optical system. The optical system is composed of lasers, optical lenses, optical fibers, filters, and light detectors. The laser emits laser light to illuminate the sample at the outlet of the flow chamber. When the particles in the sample pass through the laser, the laser light irradiating the light detector will generate The sensing signal is obtained by changing the detection speed. The information of the sample can be obtained by analyzing the sensing signal. The speed of the sample flow can be controlled by controlling the speed at which the plunger of the quantitative pump pushes the sample, thus forming various detection speeds from "low speed" to "high speed". The current problem is that the lower the detection speed, the smaller the sample flow diameter, and the more centered the particles are in the flow chamber; the higher the detection speed, the larger the sample flow diameter, and the worse the centeredness of the particle arrangement in the flow chamber, thereby reducing the precision of the signal intensity of the detection result. Therefore, if you pursue better detection precision, choose low-speed detection, and if you want a faster detection speed, choose high-speed detection. You cannot have both detection speed and detection accuracy. Summary of the invention

[0003] The embodiment of the present invention provides a flow cytometer and a working method thereof. After the sample is injected, the sample is analyzed by an optical system. At the same time, the flow rate of the sample output from the infusion tube to the sample output tube and the flow rate of the sheath fluid output from the sheath fluid pump to the flow chamber are respectively obtained by a first flow meter and a second flow meter. By analyzing the information obtained by the optical system, the operating speeds of the quantitative pump and the sheath fluid pump are adjusted, so that the flow cytometer can maximize the detection speed while maintaining the detection accuracy, thereby solving the problem that the detection speed cannot maintain the detection accuracy when the detection speed is increased in the current flow cytometer.

[0004] A flow cytometer, comprising:

[0005] A host, the host comprising a sample injection system, a sheath fluid delivery system, a flow chamber, an optical system and a controller;

[0006] The sample injection system and the sheath liquid delivery system are respectively connected to the flow chamber, the optical system is arranged at the outlet of the flow chamber, and is used to detect the sample at the outlet of the flow chamber, and the controller is respectively connected to the sample injection system and the sheath liquid delivery system for communication;

[0007] A liquid storage system, the liquid storage system comprising a liquid storage table, a sheath liquid barrel, a waste liquid barrel and a cleaning liquid bottle;

[0008] Wherein, the sheath fluid barrel, the cleaning fluid bottle and the waste fluid barrel are sequentially arranged on the liquid storage table, the sheath fluid barrel is connected to the sheath fluid delivery system through a pipeline, the waste fluid barrel is connected to the outlet of the flow chamber through a pipeline, and the cleaning fluid bottle is connected to the injection system through a pipeline;

[0009] An analysis system, applied to the flow cytometer, comprises a signal acquisition module, a signal analysis module and an adjustment module;

[0010] The signal acquisition module is used to acquire data from the injection system and the sheath fluid delivery system, and is also used to acquire detection data acquired by the optical system;

[0011] The signal analysis module is used to analyze the data collected by the signal acquisition module to obtain analysis results;

[0012] The adjustment module is used to adjust the injection system and the sheath fluid delivery system according to the analysis results obtained by the signal analysis module.

[0013] Furthermore, the sampling system includes a quantitative pump, a liquid storage pipeline, a three-way adapter, a sampling needle, a valve, a sample output tube and a first flow meter, the sampling needle is connected to the first interface of the valve through a pipeline, the second interface of the valve is connected to the first interface of the three-way adapter through a pipeline, the third interface of the valve is connected to the cleaning liquid bottle through a pipeline, the quantitative pump is connected to the second interface of the three-way adapter through the liquid storage pipeline, the third interface of the three-way adapter is connected to the input end of the sample output tube through a pipeline, the output end of the sample output tube is connected to the input end of the flow chamber, the first flow meter is arranged between the liquid storage pipeline and the input end of the sample output tube, and is used to obtain the flow rate of the sample output from the infusion tube to the sample output tube, and a check valve is arranged at the third interface of the three-way adapter.

[0014] Furthermore, the sheath fluid delivery system includes a sheath fluid pump and a second flow meter, the input end of the sheath fluid pump is connected to the sheath fluid channel through a pipeline, the output end of the sheath fluid pump is connected to the input end of the flow chamber through a pipeline, and the second flow meter is arranged between the sheath fluid pump and the flow chamber, and is used to obtain the flow rate of sheath fluid output to the flow chamber by the sheath fluid pump. The sheath fluid output to the flow chamber by the sheath fluid pump wraps the sample output to the flow chamber by the sample output tube.

[0015] Furthermore, the sheath fluid pump is a peristaltic pump, and the metering pump is a syringe pump.

[0016] Furthermore, the input end of the controller is respectively connected to the first flowmeter, the second flowmeter and the optical system for communication, and the output end of the controller is respectively connected to the metering pump, the sheath fluid pump and the signal input end of the valve for communication.

[0017] Furthermore, the signal acquisition module includes a first collector, a second collector and a third collector, the first collector is used to collect data from the first flow meter, the second collector is used to collect data from the second flow meter, and the third collector is used to collect data from the optical system.

[0018] Furthermore, the adjustment module is used to adjust the output flow rates of the sheath fluid pump and the metering pump.

[0019] In a second aspect, an embodiment of the present invention provides a working method of a flow cytometer, comprising the following steps:

[0020] S1, first cleaning, open the third interface of the valve, the second interface of the valve and the metering pump through the controller, the metering pump sucks the cleaning liquid in the cleaning liquid bottle into the liquid storage pipeline through the third interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence, close the valve, the metering pump outputs the cleaning liquid sucked from the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber, synchronously, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the cleaning liquid in the flow chamber enters the waste liquid barrel through the pipeline, the metering pump is disconnected from the cleaning liquid bottle, and the internal cleaning liquid is emptied through the sheath liquid pump and the metering pump;

[0021] S2, sample injection, insert the sampling needle into the sample, the sheath liquid pump is connected to the sheath liquid barrel, and the sheath liquid pump, valve and quantitative pump are opened by the controller. First, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, and the sheath liquid in the flow chamber enters the waste liquid barrel through the pipeline. The second flowmeter obtains the flow rate of the sheath liquid entering the flow chamber. Secondly, the quantitative pump sucks the sample into the liquid storage pipeline through the first interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence through the sampling needle. The valve is closed, and the quantitative pump outputs the sample sucked in the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber. The sheath liquid drives the sample through the outlet of the flow chamber, and the optical system obtains the information of the sample. The sheath liquid and the sample are input into the waste liquid barrel through the pipeline;

[0022] S3, system analysis, the first collector, the second collector and the third collector respectively collect data from the first flow meter, the second flow meter and the optical system, and the signal analysis module obtains analysis results by analyzing the data of the optical system;

[0023] S4, adjustment, the adjustment module adjusts the quantitative pump and the sheath liquid pump according to the analysis results and the data of the first flow meter and the second flow meter collected by the first collector and the second collector, respectively, so as to maximize the detection speed while maintaining the detection accuracy;

[0024] S5, the second cleaning, after the sample detection is completed, the third interface of the valve, the second interface of the valve and the metering pump are opened through the controller, the metering pump sucks the cleaning liquid in the cleaning liquid bottle into the liquid storage pipeline through the third interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence, the valve is closed, the metering pump outputs the cleaning liquid sucked from the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber, synchronously, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the cleaning liquid in the flow chamber enters the waste liquid barrel through the pipeline, the metering pump is disconnected from the cleaning liquid bottle, and the internal cleaning liquid is emptied through the sheath liquid pump and the metering pump.

[0025] The beneficial effects of the above technical solution provided by the embodiment of the present invention include at least:

[0026] After the sample is injected, the sample is analyzed by the optical system. At the same time, the flow rate of the sample output from the infusion tube to the sample output tube and the flow rate of the sheath fluid output from the sheath fluid pump to the flow chamber are respectively obtained by the first flow meter and the second flow meter. By analyzing the information obtained by the optical system, the operating speeds of the quantitative pump and the sheath fluid pump are adjusted, so that the flow cytometer can maximize the detection speed while maintaining the detection accuracy, thereby solving the problem that the detection speed cannot be maintained when the detection accuracy is increased in the current flow cytometer.

[0027] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the written description, claims, and drawings.

[0028] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0030] Figure 1 A schematic diagram of the structure of a flow cytometer disclosed in an embodiment of the present invention;

[0031] Figure 2 A schematic diagram of the structure of an analysis system disclosed in an embodiment of the present invention;

[0032] Figure 3 The present invention is a schematic diagram of a flow chart of a working method of a flow cytometer disclosed in an embodiment of the present invention.

[0033] Reference numerals:

[0034] 1. Main unit; 11. Sampling system; 111. Dosing pump; 112. Liquid storage pipeline; 113. Three-way adapter; 114. Sampling needle; 115. Valve; 116. Sample output tube; 117. First flow meter; 12. Sheath liquid delivery system; 121. Sheath liquid pump; 122. Second flow meter; 13. Flow chamber; 14. Optical system; 15. Controller; 16. Cell suspension concentration sensor; 2. Liquid storage system; 21. Liquid storage table; 22. Sheath liquid barrel; 23. Waste liquid barrel; 24. Cleaning liquid bottle; 3. Analysis system; 31. Signal acquisition module; 311. First collector; 312. Second collector; 313. Third collector; 32. Signal analysis module; 33. Adjustment module; 34. Sample concentration acquisition module; 35. Control parameter preset module. Specific embodiments

[0035] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0036] See attached Figure 1-2As shown, an embodiment of the present invention provides a flow cytometer, which includes a host 1, a liquid storage system 2 and an analysis system 3. After the host 1 injects a sample, the sample is analyzed by the optical system 14 inside the host 1. At the same time, the flow rate of the sample output from the infusion tube to the sample output tube 116 and the flow rate of the sheath liquid output from the sheath liquid pump 121 to the flow chamber 13 are respectively obtained by the first flow meter 117 and the second flow meter 122. The analysis system 3 analyzes the information obtained by the optical system 14 and adjusts the operating speeds of the quantitative pump 111 and the sheath liquid pump 121, so that the flow cytometer maximizes the detection speed while maintaining the detection accuracy, thereby solving the problem that the current flow cytometer cannot maintain the detection accuracy when the detection speed is increased.

[0037] See attached Figure 1-2As shown, in an embodiment of the present invention, the host 1 includes a sampling system 11, a sheath liquid delivery system 12, a flow chamber 13, an optical system 14 and a controller 15. The host 1 also includes a cell suspension concentration sensor 16, which is arranged between the sampling needle tube 114 and the valve 115, and is used to obtain the concentration of the sample. The sampling system 11 and the sheath liquid delivery system 12 are respectively connected to the flow chamber 13. The optical system 14 is arranged at the outlet of the flow chamber 13, and is used to detect the sample at the outlet of the flow chamber 13. The controller 15 is respectively connected to the sampling system 11 and the sheath liquid delivery system 12 for communication. The sampling system 11 includes a quantitative pump 111, a liquid storage pipeline 112, a three-way adapter 113, a sampling needle tube 114, a valve 115, a sample output pipe 116 and a first flow meter 117, the sampling needle tube 114 is connected to the input port of the valve 115 through a pipeline, the output port of the valve 115 is connected to the first interface of the three-way adapter 113 through a pipeline, the quantitative pump 111 is connected to the second interface of the three-way adapter 113 through the liquid storage pipeline 112, the third interface of the three-way adapter 113 is connected to the input end of the sample output tube 116 through a pipeline, the output end of the sample output tube 116 is connected to the input end of the flow chamber 13, the first flow meter 117 is arranged between the liquid storage pipeline 112 and the input end of the sample output tube 116, and is used to obtain the flow rate of the sample output from the infusion tube to the sample output tube 116, and the third interface of the three-way adapter 113 is provided with a check valve to prevent the sample and sheath liquid in the flow chamber 13 from flowing back, and the sheath liquid The delivery system 12 includes a sheath liquid pump 121 and a second flow meter 122. The input end of the sheath liquid pump 121 is connected to the sheath liquid through a pipeline, and the output end of the sheath liquid pump 121 is connected to the input end of the flow chamber 13 through a pipeline. The second flow meter 122 is arranged between the sheath liquid pump 121 and the flow chamber 13, and is used to obtain the flow rate of the sheath liquid outputted to the flow chamber 13 by the sheath liquid pump 121. The sheath liquid outputted to the flow chamber 13 by the sheath liquid pump 121 wraps the sample outputted to the flow chamber 13 by the sample output tube 116. The sheath liquid pump 121 is a peristaltic pump, and the quantitative pump 111 is a syringe pump. The input end of the controller 15 is respectively connected to the first flow meter 117, the second flow meter 122 and the optical system 14 for communication, and the output end of the controller 15 is respectively connected to the quantitative pump 111 and the sheath liquid pump 1 21 and the signal input end of the valve 115 are communicatively connected, the liquid storage system 2 includes a liquid storage table 21, a sheath liquid barrel 22, a waste liquid barrel 23 and a cleaning liquid bottle 24, the sheath liquid barrel 22, the cleaning liquid bottle 24 and the waste liquid barrel 23 are sequentially arranged on the liquid storage table 21, the sheath liquid barrel 22 is connected with the sheath liquid delivery system 12 through a pipeline, the waste liquid barrel 23 is connected with the outlet of the flow chamber 13 through a pipeline, the cleaning liquid bottle 24 is connected with the injection system 11 through a pipeline, the sampling needle 114 is inserted into the sample, the sheath liquid pump 121 is connected with the sheath liquid barrel 22, the sheath liquid pump 121, the valve 115 and the quantitative pump 111 are opened by the controller 15, firstly, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, and the sheath liquid in the flow chamber 13 enters the waste liquid barrel 23 through the pipeline,The second flowmeter 122 obtains the flow rate of the sheath liquid entering the flow chamber 13. Secondly, the quantitative pump 111 sucks the sample into the liquid storage pipeline 112 through the first interface of the valve 115, the second interface of the valve 115, the first interface of the three-way adapter 113, and the second interface of the three-way adapter 113 through the sampling needle 114. The valve 115 is closed, and the quantitative pump 111 outputs the sample sucked from the liquid storage pipeline 112 to the sample output pipe 116 through the third interface of the three-way adapter 113 and enters the flow chamber 13. The sheath liquid drives the sample through the outlet of the flow chamber 13. The optical system 14 obtains the information of the sample. The sheath liquid and the sample are input into the waste liquid barrel 23 through the pipeline. The analysis system 3 analyzes the information obtained by the optical system 14 and adjusts the running speed of the quantitative pump 111 and the sheath liquid pump 121, so that the flow cytometer can maximize the detection speed while maintaining the detection accuracy, which solves the problem that the current flow cytometer cannot maintain the detection accuracy when the detection speed is increased.

[0038] Refer to the attached Figure 1-2 As shown, in the embodiment of the present invention, the analysis system 3 is arranged on the controller 15, applied to the flow cytometer, and includes a signal acquisition module 31, a signal analysis module 32 and an adjustment module 33;

[0039] The signal acquisition module 31 is used to acquire data from the sample injection system 11 and the sheath liquid delivery system 12 , and is also used to acquire detection data acquired by the optical system 14 .

[0040] Specifically, the signal acquisition module 31 includes a first collector 311 , a second collector 312 and a third collector 313 . The first collector 311 is used to collect data from the first flow meter 117 , the second collector 312 is used to collect data from the second flow meter 122 , and the third collector 313 is used to collect data from the optical system 14 .

[0041] The signal analysis module 32 is used to analyze the data collected by the signal acquisition module 31 to obtain analysis results. The adjustment module 33 is used to adjust the injection system 11 and the sheath liquid delivery system 12 according to the analysis results obtained by the signal analysis module 32. The adjustment module 33 is used to adjust the output flow rates of the sheath liquid pump 121 and the quantitative pump 111;

[0042] Specifically, the signal analysis module 32 obtains the data of the first flow meter 117 and the second flow meter 122, and analyzes the data of the optical system 14 at the same time. The specific analysis process is: the quantitative pump 111 runs at full speed when outputting samples and the sheath liquid pump 121 outputs sheath liquid. At this time, the flow rate obtained by the first collector 311 and the second collector 312 is the maximum value. The signal analysis module 32 analyzes the waveform of the light detector signal in the optical system 14. When the waveform is unstable, the adjustment module 33 adjusts the output flow rates of the sheath liquid pump 121 and the quantitative pump 111 respectively, so that the flow rate output by the sheath liquid pump 121 is greater than the output flow rate of the quantitative pump 111, so as to avoid the sample When the sample is output from the sample output tube 116 to the flow chamber 13, accumulation occurs, causing the sample flow diameter to increase, making the waveform unstable. After the waveform is stable, the adjustment module 33 continues to adjust the output flow rates of the sheath liquid pump 121 and the metering pump 111 respectively, while ensuring that the flow rate output by the sheath liquid pump 121 is greater than the output flow rate of the metering pump 111, and at the same time, the waveform obtained by the signal analysis module 32 is stable, and the spacing between the peaks is minimized. At this time, the output flow rates of the metering pump 111 and the sheath liquid pump 121 ensure that the sample flow diameter is minimized, and the particles are arranged most centrally in the flow chamber 13, thereby achieving the effect of maximizing the detection speed while maintaining the detection accuracy.

[0043] The analysis system 3 also includes a sample concentration acquisition module 34 and a control parameter preset module 35. The sample concentration acquisition module 34 collects the concentration data of the sample obtained by the cell suspension concentration sensor 16. The control parameter preset module 35 obtains the concentration of the current sample and the parameters of the sheath pump 121 and the metering pump 111 currently adjusted by the adjustment module 33 and stores them. When samples of the same concentration are detected, the stored parameters are output to the adjustment module 33. The adjustment module 33 adjusts the output flow of the metering pump 111 and the sheath pump 121 according to the received parameters for rapid adjustment. At the same time, the adjustment module 33 adjusts the output flow of the metering pump 111 and the sheath pump 121 according to the received parameters. The analysis result is adjusted. When the analysis result shows that the waveform is stable and the spacing between the peaks is minimized, the adjustment module 33 does not make any adjustments. When other analysis results appear, the adjustment module 33 continues to adjust the output flow rates of the sheath fluid pump 121 and the metering pump 111 respectively, while ensuring that the flow rate output by the sheath fluid pump 121 is greater than the output flow rate of the metering pump 111, the waveform obtained by the signal analysis module 32 is stable and the spacing between the peaks is minimized. At the same time, the adjustment parameters of the adjustment module 33 at this time are updated to the control parameter preset module 35, which solves the problem that the detection speed of the current flow cytometer cannot maintain the detection accuracy when the detection speed is increased.

[0044] Refer to the attached Figure 1-3 As shown, the present invention also provides a working method of a flow cytometer, comprising the following steps:

[0045] S1, first cleaning, the third interface of the valve 115, the second interface of the valve 115 and the metering pump 111 are opened through the controller 15, the metering pump 111 sucks the cleaning liquid in the cleaning liquid bottle 24 into the liquid storage pipeline 112 through the third interface of the valve 115, the second interface of the valve 115, the first interface of the three-way adapter 113, and the second interface of the three-way adapter 113 in sequence, the valve 115 is closed, the metering pump 111 outputs the cleaning liquid sucked from the liquid storage pipeline 112 through the third interface of the three-way adapter 113 to the sample output tube 116 and enters the flow chamber 13, synchronously, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, the cleaning liquid in the flow chamber 13 enters the waste liquid barrel 23 through the pipeline, the metering pump 111 is disconnected from the cleaning liquid bottle 24, and the internal cleaning liquid is emptied through the sheath liquid pump 121 and the metering pump 111;

[0046] S2, sample injection, insert the sampling needle tube 114 into the sample, the sheath liquid pump 121 is connected to the sheath liquid barrel 22, the sheath liquid pump 121, the valve 115 and the quantitative pump 111 are opened by the controller 15, firstly, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, the sheath liquid pump 121 runs at full speed, outputs the maximum flow rate, the sheath liquid in the flow chamber 13 enters the waste liquid barrel 23 through the pipeline, the second flowmeter 122 obtains the flow rate of the sheath liquid entering the flow chamber 13, and secondly, the quantitative pump 111 injects the sample into the waste liquid barrel 23 in sequence through the sampling needle tube 114. The sample is sucked into the liquid storage pipeline 112 through the valve 115, the first interface of the three-way adapter 113, and the second interface of the three-way adapter 113. The valve 115 is closed, and the metering pump 111 runs at full speed to output the maximum flow rate. The metering pump 111 outputs the sample sucked into the liquid storage pipeline 112 through the third interface of the three-way adapter 113 to the sample output pipe 116 and enters the flow chamber 13. The sheath liquid drives the sample through the outlet of the flow chamber 13. The optical system 14 obtains the information of the sample. The sheath liquid and the sample are input into the waste liquid bucket 23 through the pipeline.

[0047] S3, system analysis, the first collector 311, the second collector 312 and the third collector 313 respectively collect data from the first flow meter 117, the second flow meter 122 and the optical system 14, and the signal analysis module 32 obtains analysis results by analyzing the data of the optical system 14;

[0048] S4, adjustment, the adjustment module 33 adjusts the quantitative pump 111 and the sheath liquid pump 121 according to the analysis results and the data of the first flow meter 117 and the second flow meter 122 collected by the first collector 311 and the second collector 312, respectively, so as to maximize the detection speed while maintaining the detection accuracy;

[0049] S5, the second cleaning, after the sample detection is completed, the third interface of the valve 115, the second interface of the valve 115 and the metering pump 111 are opened through the controller 15, and the metering pump 111 sucks the cleaning liquid in the cleaning liquid bottle 24 into the liquid storage pipeline 112 through the third interface of the valve 115, the second interface of the valve 115, the first interface of the three-way adapter 113, and the second interface of the three-way adapter 113 in sequence, and the valve 115 is closed, and the metering pump 111 outputs the cleaning liquid sucked from the liquid storage pipeline 112 through the third interface of the three-way adapter 113 to the sample output tube 116 and enters the flow chamber 13. Synchronously, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, and the cleaning liquid in the flow chamber 13 enters the waste liquid barrel 23 through the pipeline, and the metering pump 111 is disconnected from the cleaning liquid bottle 24, and the internal cleaning liquid is emptied through the sheath liquid pump 121 and the metering pump 111.

[0050] Specifically, after the sample is injected, the sample is analyzed by the optical system 14. At the same time, the flow rate of the sample output from the infusion tube to the sample output tube 116 and the flow rate of the sheath fluid output from the sheath fluid pump 121 to the flow chamber 13 are respectively obtained by the first flow meter 117 and the second flow meter 122. By analyzing the information obtained by the optical system 14, the operating speeds of the quantitative pump 111 and the sheath fluid pump 121 are adjusted, so that the flow cytometer can maximize the detection speed while maintaining the detection accuracy, thereby solving the problem that the current flow cytometer cannot maintain the detection accuracy when increasing the detection speed.

[0051] It should be noted that the specific models and specifications of the metering pump 111, valve 115, first flow meter 117, sheath liquid pump 121, second flow meter 122 and controller 15 need to be selected and determined according to the actual specifications of the device, and the specific selection calculation method adopts the existing technology in this field, so it will not be described in detail.

[0052] The power supply and principles of the metering pump 111, the valve 115, the first flowmeter 117, the sheath liquid pump 121, the second flowmeter 122 and the controller 15 are clear to those skilled in the art and will not be described in detail herein.

[0053] It should be understood that the specific order or hierarchy of steps in the disclosed process is an example of an exemplary method. Based on design preferences, it should be understood that the specific order or hierarchy of steps in the process can be rearranged without departing from the scope of protection of the present disclosure. The attached method claims present the elements of the various steps in an exemplary order and are not intended to be limited to the specific order or hierarchy described.

[0054] In the above detailed description, various features are grouped together in a single embodiment to simplify the disclosure. This method of disclosure should not be interpreted as reflecting an intention that the embodiments of the claimed subject matter require more features than are clearly stated in each claim. On the contrary, as reflected in the appended claims, the invention is in a state of having less than all the features of the disclosed individual embodiments. Therefore, the appended claims are hereby expressly incorporated into the detailed description, with each claim standing on its own as a separate preferred embodiment of the invention.

[0055] Those skilled in the art will also appreciate that the various illustrative logic blocks, modules, circuits, and algorithmic steps described in conjunction with the embodiments herein can all be implemented as electronic hardware, computer software, or a combination thereof. In order to clearly illustrate the interchangeability between hardware and software, various illustrative components, blocks, modules, circuits, and steps are generally described above around their functions. Whether such functions are implemented as hardware or software depends on specific applications and the design constraints imposed on the entire system. A skilled person can implement the described functions in an alternative manner for each specific application, but such implementation decisions should not be interpreted as departing from the scope of protection of the present disclosure.

[0056] The steps of the method or algorithm described in conjunction with the embodiments herein may be directly embodied as hardware, a software module executed by a processor, or a combination thereof. The software module may be located in a RAM memory, a flash memory, a ROM memory, an EPROM memory, an EEPROM memory, a register, a hard disk, a mobile disk, a CD-ROM, or any other form of storage medium well known in the art. An exemplary storage medium is connected to the processor so that the processor can read information from the storage medium and can write information to the storage medium. Of course, the storage medium may also be an integral part of the processor. The processor and the storage medium may be located in an ASIC. The ASIC may be located in a user terminal. Of course, the processor and the storage medium may also be present in a user terminal as discrete components.

[0057] For software implementation, the techniques described in this application can be implemented with modules (e.g., procedures, functions, etc.) that perform the functions described in this application. These software codes can be stored in a memory unit and executed by a processor. The memory unit can be implemented within the processor or outside the processor. In the latter case, it is coupled to the processor in a communication manner via various means, which are well known in the art.

[0058] The above description includes examples of one or more embodiments. Of course, it is impossible to describe all possible combinations of components or methods for the purpose of describing the above embodiments, but it should be recognized by those skilled in the art that the various embodiments may be further combined and arranged. Therefore, the embodiments described herein are intended to cover all such changes, modifications and variations that fall within the scope of protection of the appended claims. In addition, with respect to the term "comprising" used in the specification or claims, the word is covered in a manner similar to the term "including", just as "including," is explained as a transitional word in the claims. In addition, any term "or" used in the specification of the claims is intended to mean "non-exclusive or".

Claims

1. A flow cytometer, characterized in that: include, A host, the host comprising a sample injection system, a sheath fluid delivery system, a flow chamber, an optical system and a controller; The sample injection system and the sheath liquid delivery system are respectively connected to the flow chamber, the optical system is arranged at the outlet of the flow chamber, and is used to detect the sample at the outlet of the flow chamber, and the controller is respectively connected to the sample injection system and the sheath liquid delivery system for communication; A liquid storage system, the liquid storage system comprising a liquid storage table, a sheath liquid barrel, a waste liquid barrel and a cleaning liquid bottle; Wherein, the sheath fluid barrel, the cleaning fluid bottle and the waste fluid barrel are sequentially arranged on the liquid storage table, the sheath fluid barrel is connected to the sheath fluid delivery system through a pipeline, the waste fluid barrel is connected to the outlet of the flow chamber through a pipeline, and the cleaning fluid bottle is connected to the injection system through a pipeline; An analysis system, applied to the flow cytometer, comprises a signal acquisition module, a signal analysis module and an adjustment module; The signal acquisition module is used to acquire data from the injection system and the sheath fluid delivery system, and is also used to acquire detection data acquired by the optical system; The signal analysis module is used to analyze the data collected by the signal acquisition module to obtain analysis results; The adjustment module is used to adjust the injection system and the sheath fluid delivery system according to the analysis results obtained by the signal analysis module; The sampling system comprises a quantitative pump, a liquid storage pipeline, a three-way adapter, a sampling needle, a valve, a sample output tube and a first flow meter, the sampling needle is connected to the first interface of the valve through a pipeline, the second interface of the valve is connected to the first interface of the three-way adapter through a pipeline, the third interface of the valve is connected to the cleaning liquid bottle through a pipeline, the quantitative pump is connected to the second interface of the three-way adapter through the liquid storage pipeline, the third interface of the three-way adapter is connected to the input end of the sample output tube through a pipeline, the output end of the sample output tube is connected to the input end of the flow chamber, the first flow meter is arranged between the liquid storage pipeline and the input end of the sample output tube, and is used to obtain the flow rate of the sample output from the infusion tube to the sample output tube, the third interface of the three-way adapter is provided with a check valve, and the quantitative pump is a syringe pump; The sheath liquid delivery system comprises a sheath liquid pump and a second flow meter, the input end of the sheath liquid pump is connected to the sheath liquid channel through a pipeline, the output end of the sheath liquid pump is connected to the input end of the flow chamber through a pipeline, the second flow meter is arranged between the sheath liquid pump and the flow chamber, and is used to obtain the flow rate of the sheath liquid output to the flow chamber by the sheath liquid pump, and the sheath liquid output to the flow chamber by the sheath liquid pump wraps the sample output to the flow chamber by the sample output tube; The input end of the controller is respectively connected to the first flow meter, the second flow meter and the optical system for communication, and the output end of the controller is respectively connected to the quantitative pump, the sheath liquid pump and the signal input end of the valve for communication; The adjustment module adjusts the output flow rates of the sheath liquid pump and the metering pump respectively, so that the output flow rate of the sheath liquid pump is greater than the output flow rate of the metering pump; The host also includes a cell suspension concentration sensor, which is arranged between the sampling needle and the valve and is used to obtain the concentration of the sample; The analysis system also includes a sample concentration acquisition module and a control parameter preset module; The sample concentration acquisition module collects the concentration data of the sample acquired by the cell suspension concentration sensor, the control parameter preset module acquires the concentration of the current sample and the parameters of the sheath fluid pump and the quantitative pump currently adjusted by the adjustment module and stores them, and when samples of the same concentration are detected, the stored parameters are output to the adjustment module, and the adjustment module adjusts the output flow rates of the quantitative pump and the sheath fluid pump according to the received parameters, and performs rapid adjustment, and at the same time, the adjustment module makes adjustments according to the analysis results of the signal analysis module, and when the analysis result is that the waveform is stable and the spacing between the peaks is minimized, the adjustment module does not make adjustments, and when other analysis results appear, the adjustment module continues to adjust the output flow rates of the sheath fluid pump and the quantitative pump respectively, and while ensuring that the flow rate output by the sheath fluid pump is greater than the output flow rate of the quantitative pump, the waveform obtained by the signal analysis module is stable, and the spacing between the peaks is minimized, and at the same time, the adjustment parameters of the adjustment module at this time are updated to the control parameter preset module; The signal acquisition module includes a first collector, a second collector and a third collector, wherein the first collector is used to collect data of the first flow meter, the second collector is used to collect data of the second flow meter, and the third collector is used to collect data of the optical system; The adjustment module is used to adjust the output flow rates of the sheath fluid pump and the metering pump.

2. A flow cytometer working method, applied to the flow cytometer according to claim 1, characterized in that: The following steps are involved: S1, first cleaning, open the third interface of the valve, the second interface of the valve and the metering pump through the controller, the metering pump sucks the cleaning liquid in the cleaning liquid bottle into the liquid storage pipeline through the third interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence, close the valve, the metering pump outputs the cleaning liquid sucked from the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber, synchronously, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the cleaning liquid in the flow chamber enters the waste liquid barrel through the pipeline, the metering pump is disconnected from the cleaning liquid bottle, and the internal cleaning liquid is emptied through the sheath liquid pump and the metering pump; S2, sample injection, insert the sampling needle into the sample, the sheath liquid pump is connected to the sheath liquid barrel, and the sheath liquid pump, valve and quantitative pump are opened by the controller. First, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, and the sheath liquid in the flow chamber enters the waste liquid barrel through the pipeline. The second flowmeter obtains the flow rate of the sheath liquid entering the flow chamber. Secondly, the quantitative pump sucks the sample into the liquid storage pipeline through the first interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence through the sampling needle. The valve is closed, and the quantitative pump outputs the sample sucked in the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber. The sheath liquid drives the sample through the outlet of the flow chamber, and the optical system obtains the information of the sample. The sheath liquid and the sample are input into the waste liquid barrel through the pipeline; S3, system analysis, the first collector, the second collector and the third collector respectively collect data from the first flow meter, the second flow meter and the optical system, and the signal analysis module obtains analysis results by analyzing the data of the optical system; S4, adjustment, the adjustment module adjusts the quantitative pump and the sheath liquid pump according to the analysis results and the data of the first flow meter and the second flow meter collected by the first collector and the second collector, respectively, so as to maximize the detection speed while maintaining the detection accuracy; S5, the second cleaning, after the sample detection is completed, the third interface of the valve, the second interface of the valve and the metering pump are opened through the controller, the metering pump sucks the cleaning liquid in the cleaning liquid bottle into the liquid storage pipeline through the third interface of the valve, the second interface of the valve, the first interface of the three-way adapter, and the second interface of the three-way adapter in sequence, the valve is closed, the metering pump outputs the cleaning liquid sucked from the liquid storage pipeline through the third interface of the three-way adapter to the sample output tube and enters the flow chamber, synchronously, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the cleaning liquid in the flow chamber enters the waste liquid barrel through the pipeline, the metering pump is disconnected from the cleaning liquid bottle, and the internal cleaning liquid is emptied through the sheath liquid pump and the metering pump.

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