A luminescence detection system and method with fast results

Abstract

The present invention discloses a luminescence detection system and method that quickly produces results, belonging to the technical field of medical detection devices. The system comprises: a dispatch vehicle with a heating function; the dispatch vehicle is configured to transfer a reaction cup from an input end to an output end; a reagent mixing mechanism disposed at the output end of the dispatch vehicle; a sampling mechanism comprising: a sampling portion and a moving portion connected to the sampling portion; the sampling portion, driven by the moving portion, transfers the sample in the reagent mixing mechanism to the reaction cup; a luminescence detection mechanism disposed on one side of the reagent mixing mechanism; the luminescence detection mechanism comprises: an incubation area, a magnetic separation area, a detection area, and a waste liquid treatment area. Through spatial and temporal improvements, the present invention controls the entire detection process to within 10 minutes.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical detection devices, and in particular relates to a luminescence detection system and method capable of quickly producing results. Background Art

[0002] The main principle of chemiluminescence detection technology is to use chemiluminescence reagents to directly label antigens and antibodies in samples for analysis. During the detection process, it is necessary to control the addition of samples, reagents and incubation time.

[0003] Current testing methods take 18 minutes to produce test results. This is especially true for patients in chest pain centers and outpatient clinics, who should ensure results are available within 20 minutes of blood draw. This 18-minute timeframe is primarily due to the sample's incubation time in the incubation area, as the antigen-antibody reaction must proceed at an appropriate temperature, typically 37°C, requiring time for full reaction. Consequently, patients face extended wait times, which can increase their anxiety. Summary of the Invention

[0004] In order to solve the technical problems existing in the above-mentioned background technology, the present invention provides a luminescence detection system and method with fast results.

[0005] The present invention adopts the following technical solution: a luminescence detection system with fast results, comprising:

[0006] A dispatching vehicle having a heating function; the dispatching vehicle is configured to transfer the cuvette from the input end to the output end;

[0007] A reagent mixing mechanism is provided at the output end of the dispatching vehicle;

[0008] The sampling mechanism comprises: a sampling portion and a moving portion connected to the sampling portion; the sampling portion transfers the sample in the reagent mixing mechanism into the reaction cup under the drive of the moving portion;

[0009] A luminescence detection mechanism is provided on one side of the reagent mixing mechanism; the luminescence detection mechanism comprises: an incubation area, a magnetic separation area, a detection area and a waste liquid treatment area;

[0010] The luminescence detection mechanism also includes:

[0011] The two-dimensional transfer clamp is arranged above the incubation area, the magnetic separation area and the dispatching vehicle; the two-dimensional transfer clamp is used to realize the transfer of the reaction cup between the dispatching vehicle, the incubation area and the magnetic separation area.

[0012] In a further embodiment, the dispatch vehicle comprises:

[0013] The vehicle body has a reciprocating mechanism disposed laterally on one side thereof;

[0014] A base is transmission-connected to the reciprocating mechanism; the upper surface of the base is recessed downward to a predetermined depth from top to bottom to form a plurality of grooves, and the grooves are used to place reaction cups;

[0015] a heating element, disposed at the bottom of the base;

[0016] The guide member is arranged between the base and the vehicle body; one side of the guide member is fixed to the vehicle body, and the other side is transmission-connected to the base.

[0017] In a further embodiment, the reagent mixing mechanism comprises:

[0018] A rotating mechanism is provided on one side of the dispatching vehicle;

[0019] a turntable, drivingly connected to the rotating mechanism;

[0020] A plurality of placement slots are distributed on the turntable in a circular array with the axis of the turntable as the center;

[0021] Multiple reagent kits are positioned in the placement slots by snap-fitting.

[0022] In a further embodiment, the kit further comprises:

[0023] A hollow column, fixed to the reagent kit;

[0024] The magnetic bead bottle is inserted into the hollow column; the bottom of the hollow column extends to the outside of the hollow column and is connected to a rotating gear; the rotating gear is transmission-connected to the rotating mechanism.

[0025] In a further embodiment, the incubation zone comprises:

[0026] cavity;

[0027] An incubation tray is rotatably disposed in the cavity; the top of the incubation tray is recessed to a predetermined depth from top to bottom to form a plurality of incubation tanks; the incubation tanks are configured to accommodate reaction cups;

[0028] A first rotation source is connected to the incubation tray via a transmission mechanism; the first rotation source drives the incubation tray to rotate;

[0029] The heating element is arranged on the lower surface of the incubation tray.

[0030] In a further embodiment, the magnetic separation zone comprises:

[0031] a lifting mechanism fixed to the outer wall of the cavity;

[0032] A mounting member, transmission-connected to the lifting mechanism;

[0033] a cleaning assembly, mounted on the edge of the mounting member;

[0034] The adsorption component is arranged between the cavity and the incubation plate; the cleaning component is adapted to the adsorption component to complete multi-stage cleaning and magnetic separation of the sample.

[0035] In a further embodiment, the waste liquid treatment area includes: a waste liquid needle for sucking out the waste liquid after detection.

[0036] In a further embodiment, the cleaning assembly comprises:

[0037] at least two sets of needle assemblies mounted at the edge of the mounting member at predetermined intervals;

[0038] The needle assembly includes: a liquid injection needle having a hollow interior; a liquid injection portion connected to the top of the liquid injection needle;

[0039] The pipetting needle axially penetrates the interior of the injection needle; the top and bottom of the pipetting needle are exposed to the injection needle; and the top of the injection needle is sealed to the outer wall of the pipetting needle.

[0040] A luminescence detection method with rapid results comprises the following steps:

[0041] Step 1: Equipment initialization: Place a corresponding number of empty cuvettes on the base in the dispatching vehicle according to the number of tanks, and start the heating element to preheat the empty cuvettes;

[0042] At the same time, the reagent mixing mechanism maintains continuous mixing of the reagents inside the test kit;

[0043] Step 2: The reciprocating mechanism drives the base to transfer the empty cuvette from the input end to the output end, and then the sampling mechanism injects the reagents of the test kit into the empty cuvette according to the test requirements; the time from step 1 to step 2 is recorded as t1;

[0044] Step 3: The reciprocating mechanism drives the base to move the cuvette containing the reagent to the position of the two-dimensional transfer gripper. The two-dimensional transfer gripper places the cuvette containing the reagent into the magnetic bead bottle of the reagent kit to achieve re-mixing of the reagent. The time for executing step 3 is recorded as t2.

[0045] Step 4: The two-dimensional transfer gripper moves the mixed reaction cup into the incubation area, and the sample enters the incubation cycle. The time for executing step 4 is recorded as t3;

[0046] Step 5: After the incubation is completed, the two-dimensional transfer gripper dispatches the reaction cup to the magnetic separation area for a multi-stage cleaning and separation cycle. The time for executing step 5 is recorded as t4;

[0047] Step 6: Inject acid solution and alkali solution in sequence. After the injection is completed, start collecting photons for 3 seconds to obtain the luminescence value, take the waste liquid, and record the time of executing step 6 as t5.

[0048] In a further embodiment, the entrance and exit of the incubation zone, the entrance of the magnetic separation zone, and the reaction cup clamping points corresponding to the reagent mixing mechanism are located on the same baseline, which is consistent with the lateral movement direction of the two-dimensional transfer clamp.

[0049] The beneficial effects of the present invention are as follows: a cup dispatching vehicle with a heating function is used, the temperature of the dispatching vehicle is set to 37°C, the reaction cup is placed on the dispatching vehicle in advance for preheating, and the reagent needle and the sample needle on the sample addition control structure are synchronously aspirated and injected into the reaction cup in sequence, ensuring that the sample has a warming effect when it enters the reaction cup, saving the detection time from the time dimension; the structure is compact, the incubation area and the magnetic separation and cleaning area are integrated into a whole, the temperature of the two areas are both set to 37°C, ensuring that the incubation area and the magnetic separation and cleaning area are in a constant temperature state, and also saving a certain amount of time from the spatial operation.

[0050] Through spatial and temporal improvements, the entire detection process can be controlled within 10 minutes. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 Schematic diagram of the structure of a luminescence detection system with fast results Figure 1 .

[0052] Figure 2 Schematic diagram of the structure of a luminescence detection system with fast results Figure 2 .

[0053] Figure 3 This is a structural diagram of the dispatch vehicle.

[0054] Figure 4 Schematic diagram of the structure of the reagent mixing mechanism.

[0055] Figure 5 It is a structural diagram of the luminescence detection mechanism.

[0056] Figure 6 It is a cross-sectional view of the luminescence detection mechanism.

[0057] Figure 7 A partial cross-sectional view of the needle assembly.

[0058] Figures 1 to 7The labels in the figure are: dispatching vehicle 1, reagent mixing mechanism 2, sampling mechanism 3, luminescence detection mechanism 4, two-dimensional transfer clamp 5, reaction cup 6, body 101, reciprocating mechanism 102, base 103, guide 104, rotating mechanism 201, turntable 202, placement slot 203, reagent reagent 204, magnetic bead bottle 205, rotating gear 206, fixed gear 207, cavity 4011, incubation plate 4012, incubation tank 4013, first rotation source 4014, heating element 4015, lifting mechanism 4021, mounting part 4022, cleaning component 4023, adsorption component 4024, waste liquid needle 4025, liquid injection needle 4026, heater 4027, liquid injection part 4028, liquid aspiration needle 4029, first liquid injection needle 4030, second liquid injection needle 4031, third liquid injection needle 4032. DETAILED DESCRIPTION

[0059] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0060] In order to shorten the detection period, this embodiment discloses a luminescence detection system that can quickly produce results. Figure 1 As shown, it includes: a dispatching vehicle 1, a reagent mixing mechanism 2, a sampling mechanism 3 and a luminescence detection mechanism 4. The dispatching vehicle 1 includes an input end and an output end, and is configured to transfer the reaction cup 6 from the input end to the output end. The reagent mixing mechanism 2 is located at the output end of the dispatching vehicle 1. The sampling mechanism 3 includes: a sampling part, and a moving part that is transmission-connected to the sampling part; the sampling part transfers the sample in the reagent mixing mechanism 2 to the reaction cup 6 under the drive of the moving part. In this embodiment, the sampling part adopts a sampling needle in the prior art, and the moving part adopts a two-dimensional transmission mechanism in the prior art, which is specifically manifested as a certain horizontal movement and vertical lifting and lowering. It can adopt a gear rack transmission, a threaded screw transmission and other mechanisms, so it will not be described here.

[0061] The luminescence detection mechanism 4 is provided on one side of the reagent mixing mechanism 2; the luminescence detection mechanism 4 includes: an incubation area, a magnetic separation area, a detection area and a waste liquid treatment area; and further includes:

[0062] The two-dimensional transfer clamp 5 is arranged above the incubation area, the magnetic separation area and the dispatching vehicle 1; the two-dimensional transfer clamp 5 is used to realize the transfer of the reaction cup 6 between the dispatching vehicle 1, the incubation area, and the magnetic separation area.

[0063] In a further embodiment, Figure 3As shown, the dispatch vehicle 1 comprises a vehicle body 101. A transverse reciprocating mechanism 102 is disposed along one side of the vehicle body 101 along its length. A base 103 is drivingly connected to the reciprocating mechanism 102. The upper surface of the base 103 is recessed downward to a predetermined depth from top to bottom to form a plurality of grooves for accommodating cuvettes 6. In this embodiment, the reciprocating mechanism 102 utilizes a conveyor belt, and therefore is not described in detail here. To preheat the cuvettes 6, a heating element is also provided at the bottom of the base 103. Heating is achieved using conventional heating elements. Temperature monitoring, feedback, and control are achieved using a temperature sensor. Overtemperature protection is provided using an overtemperature switch (SEKI-ST-22), and therefore is not described here in detail.

[0064] In another embodiment, because a conveyor belt is used, there will be a certain amount of shaking during transportation. Therefore, in order to ensure the stability of the base 103 during movement, a guide member 104 is further provided between the base 103 and the body 101. In this embodiment, the guide member 104 is a guide rail provided along the length direction of the base 103, and the guide rail is fixed on the body 101. At the same time, a slidable fixed block is provided on the guide rail, and the fixed block is fixed on the base 103. The transmission connection between the body 101 and the base 103 is realized through the concave-convex cooperation between the guide rail and the fixed block.

[0065] In a further embodiment, the reagent mixing mechanism 2 includes: a rotating mechanism 201 provided on one side of the dispatching vehicle 1. For example, the rotating mechanism 201 in this embodiment is: a mounting frame that serves as a support, a column perpendicular to the mounting frame, a rotatable rotating shaft running through the interior of the column, and the top of the rotating shaft is fixedly connected to the turntable 202. It also includes: a rotating motor installed at the bottom of the mounting frame, a driving wheel connected to the rotating motor, a driven wheel fixed to the bottom end of the rotating shaft, and the driven wheel and the driving wheel are meshed with each other. The rotation of the turntable 202 is controlled by the driving motor.

[0066] Meanwhile, the turntable 202 is provided with a plurality of placement slots 203 arranged in a circular array centered on the axis of the turntable 202. Reagent kits 204 are secured within these placement slots 203. To ensure efficient mixing, particularly of samples in the reaction cup 6, in another embodiment, the reagent kit 204 further comprises: a hollow column secured to the reagent kit 204; a magnetic bead bottle 205 inserted into the hollow column; a bottom portion of the hollow column extending to the exterior of the hollow column and connected to a rotating gear 206; and the rotating gear 206 is in driving connection with the rotating mechanism 201.

[0067] Based on the above description, the rotating mechanism 201 further includes a fixed gear 207 fixed to the top of the stand, which meshes with the rotating gear 206. The operating principle of this embodiment is as follows: when the turntable 202 rotates under the action of the drive motor, since the fixed gear 207 is fixed to the stand, the rotating gear 206 rotates relative to the fixed gear 207 and also rotates on its own, thereby driving the magnetic bead bottle 205 to rotate on its own, thereby achieving a mixing effect.

[0068] like Figures 5 to 6 The incubation area includes: a cavity 4011; an incubation tray 4012 is provided inside the cavity 4011, the top of which is recessed to a predetermined depth from top to bottom to form a plurality of incubation tanks 302; the incubation tanks 302 are configured to place reaction cups 6.

[0069] The apparatus further comprises a first rotation source 4014, which is transmission-connected to the incubation tray 4012. The first rotation source 4014 drives the incubation tray 4012 to rotate. Specifically, the rotating incubation tray 4012 rotates the cuvettes 6 therein to the horizontal direction specified by the transfer gripper, and then, in conjunction with the transfer gripper, the cuvettes 6 are grasped at each angle on the incubation tray 4012. In this embodiment, the first rotation source 4014 comprises a first motor, the bottom of whose output shaft is transmission-connected to a first pulley. The apparatus further comprises a first rotating shaft, the top of which is fixedly connected to the bottom surface of the incubation tray 4012. A second pulley is fixed to the first rotating shaft, which is transmission-connected to the first pulley via a transmission belt. The incubation tray 4012 rotates by the first motor, the first pulley, the second pulley, the first transmission belt, and the first rotating shaft.

[0070] At the same time, in order to create a predetermined ambient temperature, such as a 37°C insulation environment, this embodiment is achieved by providing a heating element 4015 on the lower surface of the incubation tray 4012. In this embodiment, the heating element 4015 uses a heating plate in the prior art to achieve heating, and the temperature is monitored, fed back and controlled by a temperature sensor. Over-temperature protection is achieved by an over-temperature protection switch (SEKI-ST-22), so it is not described here.

[0071] The magnetic separation zone includes a lifting mechanism 4021 and a mounting member 4022 that is connected to the lifting mechanism 4021. In this embodiment, the lifting mechanism 4021 is a conventional motor-driven mechanism that is combined with guide rails to achieve lifting in a fixed direction, so its detailed description is omitted here. Mounting member 4022 is preferably a mounting plate with a curved outer edge. A cleaning assembly 4023 is provided at the edge of mounting member 4022. An adsorption assembly 4024 is provided between the cavity 4011 and the incubation tray 4012. The cleaning assembly 4023 and adsorption assembly 4024 are compatible, completing multi-stage cleaning and magnetic separation of the sample.

[0072] Specifically, the cleaning component 4023 includes: at least two groups of needle assemblies. In this embodiment, four groups are taken as an example. The four groups of needle assemblies are installed at the edge of the mounting member 4022 at predetermined intervals. In other words, the distance between the needle assemblies of two adjacent groups is equal, which is defined as L. By using four groups of needle assemblies arranged in a row, fourth-order magnetic separation cleaning is achieved. The structure of each group of needle assemblies is the same, specifically including: a liquid injection needle 4026 with a hollow structure inside, the top of the liquid injection needle 4026 has a liquid injection part 4028 connected thereto, wherein the liquid injection part 4028 is connected to the cleaning liquid tank through a pipeline, and an electric pump is provided on the pipeline. The cleaning liquid is sucked from the cleaning liquid tank into the liquid injection needle 4026 by the electric pump, and the cleaning liquid is injected into the reaction cup 6 located in the adsorption mechanism by the liquid injection needle 4026. The apparatus further includes a pipette needle 4029 axially extending through the interior of the injection needle 4026. Both the top and bottom of the pipette needle 4029 are exposed to the injection needle 4026. This design is intended to allow liquid to flow from a high location to a low location due to gravity during injection, but to absorb as much liquid as possible from the low location during aspiration. Therefore, the bottom of the pipette needle 4029 is lower than the bottom of the injection needle 4026. Furthermore, to ensure that liquid can flow smoothly back into the waste liquid tank during aspiration, the top of the injection needle 4026 is sealed from the outer wall of the pipette needle 4029. The top of the injection tank is connected to the waste liquid tank via a pipeline, and an electric pump is installed on the pipeline.

[0073] In order to increase the intensity of cleaning, in another embodiment, the cleaning mechanism also includes: a first injection needle 4030, which is arranged between the lifting mechanism 4021 and the needle assembly; the first injection needle 4030 is used to inject part of the cleaning liquid into the reaction cup 6 in advance; used to increase the cleaning time, as a pre-cleaning, a second injection needle 4031, which is arranged between the needle assembly and the photon collection device; the second injection needle 4031 is used to inject enhancement liquid A into the reaction cup 6; in this embodiment, the enhancement liquid A provides the reagent environment required for detection of the object to be detected.

[0074] Furthermore, the third injection needle 4032 is provided between the second injection needle 4031 and the photon collection device; the third injection needle 4032 is used to inject the enhancement solution B into the reaction cup 6. In this embodiment, the enhancement solution B is used to promote luminescence for easy detection.

[0075] In a further embodiment, the cleaning component 4023 further includes: a waste liquid needle 4025, which is provided at the edge of the mounting member 4022 and is located opposite to the needle component; the waste liquid needle 4025 is used to absorb the waste liquid after detection.

[0076] Based on the above description, adsorption assembly 4024 comprises an adsorption rotor having a predetermined radial thickness, forming a bearing surface; the bearing surface is recessed downward to a predetermined depth from top to bottom, forming a plurality of adsorption grooves; and adjacent adsorption grooves are arranged at predetermined intervals. It should be noted that the predetermined intervals between adjacent adsorption grooves are equal to the distance between two adjacent groups of needle assemblies described above, both being L. In other words, the mounting grooves are designed to match the needle assemblies, achieving high-precision liquid injection and aspiration.

[0077] The adsorption components are also included, the number and position of which correspond to those of the needle assembly. In other words, the distance between each adjacent group of adsorption components is L. In this embodiment, the adsorption components are magnets for adsorbing magnetic beads in the sample.

[0078] In order to achieve fourth-order magnetic separation for the cuvettes 6 within the same adsorption tank, a transfer process is required. Therefore, the system also includes a second power source in transmission connection with the adsorption rotor. This second rotation source drives the adsorption rotor to intermittently rotate at predetermined time intervals. The adsorption rotor is rotated from the position of the lifting mechanism 4021 through the cleaning mechanism 4 to the position of the photon detection device (i.e., the pre-detection process is completed in the order of first-order separation, second-order separation, ..., fourth-order separation, addition of enhancement solution A, and addition of enhancement solution B).

[0079] In a further embodiment, the second rotation source comprises: a second motor fixed to the frame 1; a third pulley mounted on the output shaft of the second motor; and a second rotating shaft, which is sleeved onto the first rotating shaft and non-contacting therewith. The top end of the second rotating shaft is fixedly connected to the adsorption rotor, and the bottom end is rotatably mounted on the frame. A fourth pulley is fixed to the outer wall of the second rotating shaft. The fourth pulley and the third pulley are driven by a transmission belt, thereby achieving rotation of the adsorption rotor. By inputting a predetermined algorithm into the second motor, intermittent rotation is achieved at equal intervals according to distances and time intervals.

[0080] In a further embodiment, to ensure that the test results are not affected, the test object must remain within the incubator until testing is complete. This means that the environment must not experience significant temperature fluctuations. Therefore, a heater 4027 is provided beneath the adsorption rotor to provide heating. In this embodiment, heater 4027 utilizes a conventional heating plate. Temperature monitoring and control are achieved through a temperature sensor, and over-temperature protection is provided through an over-temperature switch (SEKI-ST-22). Therefore, detailed description of this design is omitted here.

[0081] In a further embodiment, the photon detection device can adopt existing technology, but it should be noted that the photon detection device scans the object to be detected inside the reaction cup 6. Therefore, in order not to affect the normal operation of the photon detection device, the outer side surface of the bottom of the adsorption groove is a hollow structure, that is, the photon detection device completes the detection of the object to be detected in the reaction cup 6 through the hollow structure.

[0082] A luminescence detection method with rapid results comprises the following steps:

[0083] Step 1: Equipment initialization: Place a corresponding number of empty cuvettes on the base in the dispatching vehicle according to the number of tanks, and start the heating element to preheat the empty cuvettes;

[0084] At the same time, the reagent mixing mechanism maintains continuous mixing of the reagents inside the test kit;

[0085] Step 2: The reciprocating mechanism drives the base to transfer the empty cuvette from the input end to the output end, and then the sampling mechanism injects the reagents of the test kit into the empty cuvette according to the test requirements; the time from step 1 to step 2 is recorded as t1; t1 is controlled to be 15 seconds;

[0086] Step 3: The reciprocating mechanism drives the base to move the cuvette containing the reagent to the position of the two-dimensional transfer gripper. The two-dimensional transfer gripper places the cuvette containing the reagent into the magnetic bead bottle of the reagent kit to achieve re-mixing of the reagent. The time for executing step 3 is recorded as t2; t2 is controlled within 7 seconds.

[0087] Step 4: The two-dimensional transfer gripper moves the mixed cuvette into the incubation zone, and the sample enters the incubation cycle. The time for executing step 4 is recorded as t3; t3 is controlled within 3 minutes, which includes the incubation time;

[0088] Step 5: After the incubation is completed, the two-dimensional transfer gripper dispatches the reaction cup to the magnetic separation area for a multi-stage cleaning and separation cycle. The time for executing step 5 is recorded as t4; t4 is controlled within 4 seconds;

[0089] Step 6: Inject acid and alkali solution in sequence. After the injection is completed, collect the number of photons for 3 seconds to obtain the luminescence value. Remove the waste liquid and record the time of executing step 6 as t5. t5 should be controlled within 30 seconds.

[0090] Adapted to the two-dimensional transfer gripper: the entrance and exit of the incubation zone, the entrance of the magnetic separation zone, and the reaction cup clamping points corresponding to the reagent mixing mechanism are located on the same baseline, which is consistent with the lateral movement direction of the two-dimensional transfer gripper.

[0091] Steps 4 to 6 specifically include the following processes:

[0092] Step 101: Place human antigens, antibodies, and designated reagents into a cuvette, which is then placed in an incubation tank within an incubation tray and incubated at a specified temperature for a predetermined time. A heating element located beneath the incubation tray maintains the temperature of the cuvette and its contents within the tray. The temperature can be controlled to 37°C.

[0093] Step 102: The first rotation source drives the incubation disk to rotate, and cooperates with the mobile gripper to transfer the reaction cup after incubation to the adsorption tank of the adsorption rotor, and controls the current reaction cup and the corresponding adsorption tank to be located at the first injection needle;

[0094] Step 103: The first injection needle injects some cleaning solution into the reaction cup. At this time, the magnet matched with the first injection needle begins to absorb the magnetic beads in the reaction cup, and starts to achieve solid-liquid separation of the substances inside the reaction cup.

[0095] In step 104, the second rotation source drives the adsorption rotor to rotate a predetermined distance, causing the current reaction cup to stop at N needle assemblies in sequence. During each stop, each needle assembly performs injection and aspiration operations on the current reaction cup. For example, during one of these stops, the current reaction cup is transferred to the location of the second needle assembly. The injection needle of the needle assembly first injects cleaning fluid from the cleaning fluid tank into the reaction cup. After a predetermined period of time, the aspiration needle of the needle assembly removes the liquid from the reaction cup to the waste liquid tank. During this process, the cleaning fluid maintains a certain fluidity within the reaction cup, while the adsorption element continuously attracts the magnetic beads. This combination increases the intensity of mixing, achieving more thorough solid-liquid separation.

[0096] Step 105: The second rotation source continues to drive the adsorption rotor to rotate a predetermined distance, and injects the enhancement liquid A and the enhancement liquid B into the current reaction cup through the second injection needle and the third injection needle respectively; the second injection needle and the third injection needle are respectively connected to the containers of the enhancement liquid A and the enhancement liquid B through pipelines, and are respectively provided with electric pumps to provide a certain external force.

[0097] Step 106: The photon detection device completes the detection through the hollow structure of the adsorption tank;

[0098] Step 107 : The second rotation source continues to drive the adsorption rotor to rotate a predetermined distance, and the waste liquid needle absorbs the waste liquid in the tested reaction cup.

[0099] The lifting and lowering of the needle cleaning area integrates the liquid injection needle, liquid aspiration needle and solid-liquid separation needle, completing the injection and extraction of the cleaning liquid and the solid-liquid separation of the waste liquid after the reaction in one action. It also reduces the use of motors and optocouplers, increases the synchronization of actions, and is more conducive to the realization of control.

Claims

1. A luminescence detection system with fast results, characterized in that: include: A dispatching vehicle having a heating function; the dispatching vehicle is configured to transfer the cuvette from the input end to the output end; A reagent mixing mechanism is provided at the output end of the dispatching vehicle; The sampling mechanism comprises: a sampling portion and a moving portion connected to the sampling portion; the sampling portion transfers the sample in the reagent mixing mechanism into the reaction cup under the drive of the moving portion; A luminescence detection mechanism is provided on one side of the reagent mixing mechanism; the luminescence detection mechanism comprises: an incubation area, a magnetic separation area, a detection area and a waste liquid treatment area; The luminescence detection mechanism also includes: A two-dimensional transfer gripper is provided above the incubation area, the magnetic separation area, and the dispatch vehicle; the two-dimensional transfer gripper is used to transfer the reaction cup between the dispatch vehicle, the incubation area, and the magnetic separation area; The reagent mixing mechanism comprises: A rotating mechanism is provided on one side of the dispatching vehicle; a turntable, drivingly connected to the rotating mechanism; A plurality of placement slots are distributed on the turntable in a circular array with the axis of the turntable as the center; Multiple test kits are positioned in the placement slots by snap-fitting; The kit further comprises: A hollow column, fixed to the reagent kit; A magnetic bead bottle is inserted into the hollow column; the bottom of the hollow column extends to the outside of the hollow column and is connected to a rotating gear; the rotating gear is in transmission connection with the rotating mechanism; the rotating mechanism also includes a fixed gear fixed to the top of the stand, the fixed gear meshing with the rotating gear; The incubation zone includes: a cavity; An incubation tray is rotatably disposed in the cavity; the top of the incubation tray is recessed to a predetermined depth from top to bottom to form a plurality of incubation tanks; the incubation tanks are configured to accommodate reaction cups; A first rotation source is connected to the incubation tray via a transmission mechanism; the first rotation source drives the incubation tray to rotate; A heating element is provided on the lower surface of the incubation tray; The magnetic separation zone comprises: a lifting mechanism fixed to the outer wall of the cavity; A mounting member, transmission-connected to the lifting mechanism; a cleaning assembly, mounted on the edge of the mounting member; The adsorption component is arranged between the cavity and the incubation plate; the cleaning component is adapted to the adsorption component to perform multi-stage cleaning and magnetic separation on the sample; The entrance and exit of the incubation zone, the entrance of the magnetic separation zone, and the reaction cup clamping points corresponding to the reagent mixing mechanism are located on the same baseline, which is consistent with the lateral movement direction of the two-dimensional transfer clamping claw.

2. A luminescence detection system with fast results according to claim 1, characterized in that: The dispatching vehicle includes: The vehicle body has a reciprocating mechanism disposed laterally on one side thereof; A base is transmission-connected to the reciprocating mechanism; the upper surface of the base is recessed downward to a predetermined depth from top to bottom to form a plurality of grooves, and the grooves are used to place reaction cups; a heating element, disposed at the bottom of the base; The guide member is arranged between the base and the vehicle body; one side of the guide member is fixed to the vehicle body, and the other side is transmission-connected to the base.

3. The luminescence detection system with fast results according to claim 1, characterized in that: The waste liquid treatment area includes: a waste liquid needle for absorbing the waste liquid after detection.

4. A luminescence detection system with rapid results according to claim 1, characterized in that: The cleaning component comprises: at least two sets of needle assemblies mounted at the edge of the mounting member at predetermined intervals; The needle assembly includes: a liquid injection needle having a hollow interior; a liquid injection portion connected to the top of the liquid injection needle; The pipetting needle axially penetrates the interior of the injection needle; the top and bottom of the pipetting needle are exposed to the injection needle; and the top of the injection needle is sealed to the outer wall of the pipetting needle.

5. A luminescence detection method using a fast-result luminescence detection system according to any one of claims 2 to 4, characterized in that: The following steps are involved: Step 1: Equipment initialization: Place a corresponding number of empty cuvettes on the base in the dispatching vehicle according to the number of tanks, and start the heating element to preheat the empty cuvettes; At the same time, the reagent mixing mechanism maintains continuous mixing of the reagents inside the test kit; Step 2: The reciprocating mechanism drives the base to transfer the empty cuvette from the input end to the output end, and then the sampling mechanism injects the reagents of the test kit into the empty cuvette according to the test requirements; the time from step 1 to step 2 is recorded as t1; Step 3: The reciprocating mechanism drives the base to move the cuvette containing the reagent to the position of the two-dimensional transfer gripper. The two-dimensional transfer gripper places the cuvette containing the reagent into the magnetic bead bottle of the reagent kit to achieve re-mixing of the reagent. The time for executing step 3 is recorded as t2. Step 4: The two-dimensional transfer gripper moves the mixed reaction cup into the incubation area, and the sample enters the incubation cycle. The time for executing step 4 is recorded as t3; Step 5: After the incubation is completed, the two-dimensional transfer gripper dispatches the reaction cup to the magnetic separation area for a multi-stage cleaning and separation cycle. The time for executing step 5 is recorded as t4; Step 6: Inject acid solution and alkali solution in sequence. After the injection is completed, start collecting photons for 3 seconds to obtain the luminescence value, take the waste liquid, and record the time of executing step 6 as t5.

6. A luminescence detection method with rapid results according to claim 5, characterized in that: The entrance and exit of the incubation zone, the entrance of the magnetic separation zone, and the reaction cup clamping points corresponding to the reagent mixing mechanism are located on the same baseline, which is consistent with the lateral movement direction of the two-dimensional transfer clamping claw.

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