A fully automatic photochemiluminescence detector

Abstract

The present invention proposes a fully automatic photochemiluminescence detector for improving the accuracy of chemical analysis, comprising a plate rack module; a pushing device; a sample loading arm module; a turntable module; a sample rack module; an incubation module; a reagent module and a detection module, wherein the slats on the plate rack module are pushed to the turntable module by the pushing device, and the sample loading arm module is used to add samples and reagents to the reaction cups on the slats on the turntable module, and the slats on the turntable module are pushed into the incubation module by the pushing device, and enter the detection module for detection after the incubation is completed. The fully automatic photochemiluminescence detector of the present invention has a simple structure, a smooth and simple detection process, high detection efficiency, and high detection accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a full-automatic photoinduced chemiluminescence detector. Background Art

[0002] At present, the existing photochemiluminescence detector is generally used to read 96-well or 48-well microplates. The so-called 96-well or 48-well microplates include 96 or 48 reaction cups on the plate, and each sample to be detected is placed in a reaction cup. When the number of samples to be detected is only a few or a dozen, a whole 96-well or 48-well microplate is also required, which causes a lot of waste. In addition, the time required for adding samples from the first well to the last well of the 96-well or 48-well microplate is relatively long, which has a certain impact on the consistency of the results. For example, the patent with the authorization announcement number CN203490229U published on March 19, 2014 discloses a quasi-automatic plate-type immunoassay analyzer, which is mainly composed of a housing, a placement unit, an incubation unit, a cleaning unit, a substrate filling unit, an optical detection unit and a system operation control unit, and realizes the entire process of reaction solution incubation, reaction solution plate washing, reaction solution self-filling and substrate measurement. Two microplates can be placed at the same time during operation. On the other hand, the existing single reaction cup on the market is used as a transfer and detection unit, and can also perform detection when there is only one sample. However, the efficiency of such single detection is not high enough, and when the number of samples is large, the detection time is relatively long, which is not suitable for large-scale inspection. For example, the patent with authorization announcement number CN102183639B published on June 5, 2013 discloses a fully automatic chemiluminescence immunoassay analyzer, which consists of a reaction cup feeding system, a three-dimensional mobile sample loading module, a sample area, a reagent area, a pump group, and a control system. The single reaction cup is used for transfer detection, so the detection efficiency is not high enough.

[0003] The above two detection methods have their own disadvantages. There is an urgent need for a photochemiluminescence detector that is between the above two methods to ensure the accuracy of the results and the efficiency of the detection. Summary of the invention

[0004] The present invention provides a fully automatic photoinduced chemiluminescence detector with simple structure, smooth and simple detection process, high detection accuracy and high detection efficiency.

[0005] The fully automatic photoluminescence detector of the present invention comprises a plate taking frame module, a pushing device, a sample loading arm module, a turntable module, a sample rack module, an incubation module, a reagent module and a detection module, wherein the turntable module comprises a turntable base and a disk body assembly, the disk body assembly comprises a turntable, a plurality of slat clamping devices are arranged on the turntable, the slat clamping device can hold a plurality of slats, the slats on the plate taking frame module are pushed to the loading area of ​​the turntable module by the Y-direction pushing mechanism of the pushing device, the sample and the reagent are added to the reaction cup on the slat located in the sample loading area of ​​the turntable module by the sample loading arm module, the X-direction pushing mechanism of the pushing device drives the slat carrying the mixed solution on the turntable module to move to the incubation module, and enters the detection module for detection after the incubation is completed;

[0006] The turntable is located behind the plate retrieval rack module, the incubation module is arranged on one side of the turntable, the sample rack module and the reagent module are respectively located on both sides of the plate retrieval rack module, and the incubation is carried out on two incubation plates to achieve different incubation times.

[0007] In one embodiment, the incubation module comprises an incubation plate and a first sliding mechanism, the incubation plate is slidably connected to the frame via the first sliding mechanism, and a slat clamping device is provided on the incubation plate.

[0008] Preferably, the incubation module includes two incubation plates arranged parallel to each other, which are respectively slidably connected to the frame through a set of first sliding mechanisms, the first sliding mechanism includes a first motor and a first slide rail, the incubation plate is arranged on the first slide rail, the first motor is connected to the incubation plate through a first synchronous belt, and the first motor rotates to drive the incubation plate to slide along the first slide rail.

[0009] In one embodiment, the slat clamping device includes a clamping base plate, a vertical plate and a slat pressing plate, the vertical plate is provided with a spring clip slot, a slat spring is provided in the spring clip slot, the outer side surface of the slat spring protrudes from the vertical side wall of the vertical plate, the slat pressing plate is fixed to the upper end surface of the vertical plate and presses the slat spring in the vertical direction, the slat is arranged between two adjacent vertical plates and is pressed in the horizontal direction by the slat spring.

[0010] In one embodiment, the pushing device includes an X-direction pushing mechanism and a Y-direction pushing mechanism. The slats on the plate rack module are pushed to the turntable module by the Y-direction pushing mechanism, and the slats on the turntable module are pushed into the incubation module by the X-direction pushing mechanism.

[0011] In one embodiment, the turntable module includes a turntable base, a disk body assembly, a shaft assembly, a turntable motor and an induction assembly. The turntable base is fixed on a frame. The disk body assembly includes a turntable, a first gear and a gear pressure plate. Four slat clamping devices symmetrically arranged on the same circle are provided on the upper surface of the turntable. The shaft assembly passes through the gear pressure plate and the first gear from bottom to top. The upper part of the shaft assembly is fixed on the lower surface of the turntable. A second gear is provided on the output end of the turntable motor, and the second gear is meshed with the first gear.

[0012] In one embodiment, the sensing component includes a turntable zero position sensor and a turntable working position sensor. A boss is provided on the lower surface of the turntable below the slat clamping device. The boss is located on the same circumference as the turntable zero position sensor and the turntable working position sensor. When the turntable rotates, the lower end of the boss intermittently passes through the turntable zero position sensor and the turntable working position sensor.

[0013] In one embodiment, the plate retrieval rack module includes a plate rack for placing slats, a stack, a plate retrieval rack mechanism and a plate rack transmission mechanism, and the plate retrieval rack mechanism is fixed to the stack through a fixed plate; the plate retrieval rack mechanism includes a first plate retrieval support plate, a second plate retrieval support plate and a second sliding mechanism, and the first plate retrieval support plate is slidably connected to the second sliding mechanism through a plate retrieval connecting plate.

[0014] In one embodiment, a rectangular opening is provided on the first plate retrieval support plate, and the second plate retrieval support plate is horizontally slidably arranged at the rectangular opening by screws, a spring is provided between the first plate retrieval support plate and the second plate retrieval support plate, and an arc-shaped protrusion is provided on the outer side of the second plate retrieval support plate, and the protrusion extends outward beyond the outer edge of the first plate retrieval support plate; two supporting ribs are provided on the lower surface of the plate rack, and a clamping rib is provided on the inner side of each of the two supporting ribs, wherein an arc-shaped depression is provided on the clamping rib on one side of the protrusion, and when the first plate retrieval support plate and the second plate retrieval support plate enter the stack and extend under the topmost plate rack, the first plate retrieval support plate and the second plate retrieval support plate are located between the two clamping ribs, and the protrusion sinks into the depression.

[0015] In one embodiment, the plate rack transmission mechanism includes a lifting motor, a plate bracket, a screw and two guide rods. The two guide rods and the screw are vertically arranged parallel to each other on one side of the stack. The two guide rods and the screw are passed through a lifting slider. The plate bracket is fixed on the lifting slider and extends into the stack. The plate rack with slats placed on it is longitudinally stacked on the plate bracket. The stacked plate rack is located inside the stack, and the lower end of the screw is connected to the lifting motor through a lifting motor synchronous belt.

[0016] In one embodiment, the second sliding mechanism includes a second motor and a second slide rail. The plate taking connecting plate is arranged on the second slide rail and slides along the second slide rail. The second motor is connected to the plate taking connecting plate through a second synchronous belt. The second motor rotates to drive the plate taking connecting plate to slide along the second slide rail.

[0017] In one embodiment, the detection module includes an optical path component, a slat transfer component, a detection base plate and a third sliding mechanism. The detection base plate is provided with a slat drop groove, and the slat transfer component is movably arranged on the upper surface of the detection base plate. The optical path component is arranged on the upper surface of the detection base plate through a slat transfer channel. When performing detection, the slats on the slat transfer assembly are located directly below the optical path component.

[0018] In one embodiment, the slat transfer assembly includes a sliding block, a slat plug-in and a DC motor, a guide rail is provided on the upper surface of the sliding block, the slat plug-in is arranged on the guide rail, the DC motor is arranged on the sliding block, and a third gear is provided at the output end, the slat plug-in is provided with a rack, the third gear and the rack are meshed with each other, the DC motor rotates and drives the slat plug-in to slide along the guide rail, and the slat plug-in is provided with a plurality of plugs arranged parallel to each other on the side close to the optical path assembly, and the slat is mounted on the plugs.

[0019] Preferably, the third sliding mechanism includes a third motor and a third slide rail, the sliding block is arranged on the third slide rail and slides along the third slide rail, the third motor is connected to the sliding block through a third synchronous belt, and the third motor rotates to drive the sliding block to slide along the third slide rail.

[0020] In one embodiment, the sample rack module includes a sample rack base plate, a test tube rack and a test tube rack adapter. The test tube rack is provided with a plurality of test tube jacks, and the test tube jacks are provided with test tube clamping plates, and test tubes containing samples are inserted into the test tube clamping plates. The sample rack base plate is provided with a plurality of groups of guide blocks, and the bottom surface of the test tube rack adapter is provided with a guide groove. The test tube rack is fixed to the test tube rack adapter, and then the test tube rack and the test tube rack adapter are inserted together from one side of the sample rack base plate.

[0021] Preferably, one and two magnetic steels are respectively provided on the front end and the lower surface of the test tube rack adapter, and the three magnetic steels are all sunken into the test tube rack adapter.

[0022] In one embodiment, the pushing device also includes a push rod base plate, the X-direction pushing mechanism and the Y-direction pushing mechanism of the pushing device have the same structure, and are slidably connected to the push rod base plate through a fourth sliding mechanism and a fifth sliding mechanism respectively, the X-direction pushing mechanism and the Y-direction pushing mechanism both include a push rod, a push rod motor and a push rod arm, a push rod slide rail is horizontally provided on the push rod arm, the push rod is slidably arranged on the push rod slide rail, and is connected to the push rod motor through a push rod synchronous belt.

[0023] Preferably, the fourth sliding mechanism includes a fourth motor and a fourth slide rail, the push rod arm is arranged on the fourth slide rail, the fourth motor is connected to the push rod arm through a fourth synchronous belt, the fourth motor rotates to drive the push rod arm to slide along the fourth slide rail, and the fifth sliding mechanism and the fourth sliding mechanism have the same structure.

[0024] In one embodiment, the fully automatic photochemiluminescence detector further includes a universal liquid module, a liquid circuit module and a dilution and oscillation module. The sample rack module, reagent module, universal liquid module and dilution and oscillation module implement the operation of adding liquid during the detection process through the liquid circuit module.

[0025] In one embodiment, a needle washing pool is provided on one side of the sample rack module and the reagent module respectively.

[0026] Compared with the prior art, the fully automatic photoluminescence detector of the present invention has the following advantages:

[0027] The fully automatic photochemiluminescence detector of the present invention comprises a housing, a frame, a strip for detection, and a plate rack module, a pushing device, a sample loading arm module, a turntable, a sample rack module, an incubation module, a reagent module and a detection module arranged on the frame. The strips for detection are placed side by side on the plate rack, and a plurality of plate racks are stacked in a stack of the plate rack module. The plate rack on the top layer is taken out by the plate rack taking mechanism, and then the strips are pushed to the strip clamping device on the turntable by the Y-direction pushing mechanism. When the turntable rotates 90 degrees clockwise, the strips reach the sample loading position for loading and dilution. At this time, push a slat from the plate rack to the slat clamping device again, and then rotate clockwise 90 degrees, push a slat every 90 degrees at the push position, add samples at the sample adding position, and then turn 90 degrees twice to reach the reagent adding position for reagent adding operation, and then push the slat into the incubation module through the X-direction pushing mechanism for incubation. The incubation module includes an incubation plate and a first sliding mechanism. The slat is fixed on the incubation plate, and then slides back and forth through the first sliding mechanism for oscillation mixing, and incubation treatment is performed at the same time, so that the detection efficiency is higher, and then enters the detection module for detection. The plate rack module facilitates the removal of the plate rack and sends it to the turntable in an orderly manner. The turntable can efficiently complete operations such as sample addition, reagent addition, and dilution. The sample rack module can conveniently and stably place the sample in the sample area. The detection module can conveniently discard the slat after detection, which is fast and flexible, thereby ensuring the efficiency of detection.

[0028] The above technical features can be combined in various suitable ways or replaced by equivalent technical features as long as the purpose of the present invention can be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The invention will be described in more detail below based on non-limiting examples and with reference to the accompanying drawings, in which:

[0030] Figure 1 This is a schematic diagram of the structure of the fully automatic photochemiluminescence detector of the present invention;

[0031] Figure 2 This is a schematic diagram of the structure of the front side of the fully automatic photochemiluminescence detector of the present invention after removing the outer shell;

[0032] Figure 3 This is a schematic diagram of the structure of the fully automatic photochemiluminescence detector of the present invention after removing the outer shell;

[0033] Figure 4 It is a structural schematic diagram of the slat clamping device of the present invention;

[0034] Figure 5 An exploded view of the slat clamping device of the present invention;

[0035] Figure 6This is a schematic structural diagram of the slat clamping device of the present invention without the slat pressing sheet;

[0036] Figure 7 It is a structural schematic diagram of the turntable module of the present invention;

[0037] Figure 8 This is an exploded view of the turntable module of the present invention;

[0038] Fig. 9 An exploded view of the turntable assembly of the present invention;

[0039] Fig.10 It is a structural schematic diagram of the plate-taking rack module of the present invention;

[0040] Fig.11 This is an exploded view of the plate-taking rack module of the present invention;

[0041] Fig.12 It is a structural schematic diagram of the plate-taking and plate-separating state of the plate-taking frame mechanism of the present invention;

[0042] Fig.13 It is a structural schematic diagram of the plate-taking and engaging state of the plate-taking frame mechanism of the present invention;

[0043] Fig.14 It is an exploded view of the plate-taking frame mechanism of the present invention;

[0044] Fig.15 It is a structural schematic diagram of the detection module of the present invention;

[0045] Fig.16 An exploded diagram of the detection module of the present invention;

[0046] Fig.17 It is a structural schematic diagram of the slat transfer assembly of the present invention;

[0047] Fig.18 It is a schematic diagram of the structure of the slat plug-in of the present invention;

[0048] Fig.19 It is a schematic structural diagram of the slat transfer channel of the present invention.

[0049] Fig. 20 A top view of the pushing device of the present invention;

[0050] Fig.21 It is a structural schematic diagram of the pushing device of the present invention;

[0051] Fig. 22 It is a structural schematic diagram of the working state of the Y-direction pushing mechanism of the present invention;

[0052] Fig.23 It is a structural schematic diagram of the working state of the X-direction pushing mechanism of the present invention;

[0053] Fig.24 It is a structural schematic diagram of the sample rack module of the present invention;

[0054] Fig.25 An exploded view of the sample rack module of the present invention with the front of the sample rack bottom plate removed;

[0055] Fig.26 This is an exploded view of the sample rack module of the present invention with the reverse side of the sample rack bottom plate removed.

[0056] The numbers corresponding to the component names in the figure are as follows:

[0057] 1. Shell; 2. Rack; 3. Slats; 4. Sample loading arm module; 5. Reagent module; 6. X-axis pushing mechanism; 7. Y-axis pushing mechanism; 8. Incubation plate; 9. First motor; 10. First slide rail; 11. First synchronous belt; 12. Vertical plate; 13. Slat pressing piece; 14. Shrapnel slot; 15. Slat shrapnel; 16. Turntable base; 17. Rotating shaft assembly; 18. Turntable motor; 19. Turntable; 20. First gear; 21. Gear pressing piece; 22. Second gear; 23. Turntable zero position sensor; 24. Turntable working position sensor; 25. convex column; 26. plate rack; 27. stack; 28. fixed plate; 29. ​​first plate taking support plate; 30. second plate taking support plate; 31. plate taking connecting plate; 32. rectangular opening; 33. screw; 34. spring; 35. convex block; 36. support rib; 37. clamping rib; 38. depression; 39. lifting motor; 40. plate bracket; 41. screw; 42. guide rod; 43. lifting slider; 44. second motor; 45. second slide rail; 46. second synchronous belt; 47 , optical path assembly; 48, detection base plate; 49, slat drop groove; 50, slat transfer channel; 51, slide block; 52, slat plug-in; 53, DC motor; 54, guide rail; 55, third gear; 56, rack; 57, insert; 58, third motor; 59, third slide rail; 60, third synchronous belt; 61, sample rack base plate; 62, test tube rack; 63, test tube rack adapter; 64, test tube jack; 65, test tube clamping piece; 66, guide block; 67, guide groove; 68, magnetic steel; 69, push Rod base plate; 70, push rod; 71, push rod motor; 72, push rod arm; 73, push rod slide rail; 74, push rod timing belt; 75, fourth motor; 76, fourth slide rail; 77, fourth timing belt; 78, universal liquid module; 79, liquid path module; 80, dilution and oscillation module; 81, needle washing pool; 82, test tube; 83, plate rack module; 84, pushing device; 85, turntable module; 86, sample rack module; 87, incubation module; 88, detection module; 89, clamping base plate; 90, slat clamping device.

[0058] In the figures, the same components are indicated by the same reference numerals. The figures are not drawn to scale. DETAILED DESCRIPTION

[0059] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, as long as there is no conflict, the various embodiments of the present invention and the various features in the embodiments can be combined with each other, and the technical solutions formed are all within the protection scope of the present invention.

[0060] like Figure 1 , Figure 2 , Figure 3 As shown, the fully automatic photochemiluminescence detector of the present invention includes a plate rack module 83, a pushing device 84, a sample loading arm module 4, a turntable module 85, a sample rack module 86, an incubation module 87, a reagent module 5 and a detection module 88, wherein the slats 3 on the plate rack module 83 are pushed to the turntable module 85 by the pushing device 84, and samples and reagents are added to the reaction cups on the slats on the turntable module 85 by the sample loading arm module 4, and the slats on the turntable module 85 are pushed into the incubation module 87 by the pushing device 84, and enter the detection module 88 for detection after the incubation is completed.

[0061] In one embodiment, the plate retrieval rack module 83 is arranged at the front of the rack 2, a shell 1 is arranged outside the rack 2, a turntable is arranged behind the plate retrieval rack module 83, an incubation module 87 is arranged on one side of the turntable, the sample rack module 86 and the reagent module 5 are respectively located on both sides of the plate retrieval rack module 83, and the pushing device 84 includes an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7. The slats on the plate retrieval rack module 83 are pushed to the turntable 19 by the Y-direction pushing mechanism 7, and the slats 3 on the turntable 19 are pushed into the incubation module 87 by the X-direction pushing mechanism 6, and enter the detection module 88 for detection after the incubation is completed.

[0062] In one embodiment, the incubation module 87 includes an incubation plate 8 and a first sliding mechanism. The incubation plate 8 is slidably connected to the frame 2 via the first sliding mechanism. A slat clamping device 90 is provided on the incubation plate 8 .

[0063] In one embodiment, the incubation module 87 includes two incubation plates 8 arranged parallel to each other, which are respectively slidably connected to the frame 2 through a set of first sliding mechanisms. The first sliding mechanism includes a first motor 9 and a first slide rail 10. The incubation plate 8 is arranged on the first slide rail 10. The first motor 9 is connected to the incubation plate 8 through a first synchronous belt 11. The first motor 9 rotates to drive the incubation plate 8 to slide along the first slide rail 10.

[0064] The incubation is carried out separately on two incubation plates 8, so that different incubation times can be achieved. The speed of rotation of the first motor 9 can determine the speed of the incubation plates 8 moving back and forth, thereby achieving different degrees of oscillation and mixing, and the operation is more flexible and varied.

[0065] like Figure 4 , Figure 5 and Figure 6 As shown, in one embodiment, the slat clamping device 90 includes a clamping bottom plate 89, a vertical plate 12 and a slat pressing piece 13. The vertical plate 12 is provided with an elastic piece slot 14, and a slat elastic piece 15 is provided in the elastic piece slot 14. The outer side surface of the slat elastic piece 15 protrudes from the vertical side wall of the vertical plate 12. The slat pressing piece 13 is fixed to the upper end surface of the vertical plate 12 and presses the slat elastic piece 15 in the vertical direction. The slat 3 is arranged between two adjacent vertical plates 12 and is pressed in the horizontal direction by the slat elastic piece 15. The slat clamping device 90 can clamp the slat 3 in the horizontal direction and the vertical direction at the same time. The slat elastic piece 15 can clamp the slat 3 horizontally, and the slat pressing piece 13 can press the slat 3 in the vertical direction, thereby making the slat 3 more stable during the movement.

[0066] like Figures 7 to 9 As shown, in one embodiment, the turntable module 85 includes a turntable base 16, a disk assembly, a shaft assembly 17, a turntable motor 18 and a sensing assembly. The turntable base 16 is fixed on the frame 2. The disk assembly includes a turntable 19, a first gear 20 and a gear pressing plate 21. Four slat clamping devices 90 symmetrically arranged on the same circumference are provided on the upper surface of the turntable 19. The shaft assembly 17 passes through the gear pressing plate 21 and the first gear 20 from bottom to top. The upper part of the shaft assembly 17 is fixed on the lower surface of the turntable 19. A second gear 22 is provided on the output end of the turntable motor 18, and the second gear 22 is meshed with the first gear 20. Each time the turntable 19 rotates 90 degrees, operations such as adding samples, adding diluents, and adding reagents are realized respectively. A plurality of slat clamping devices 90 are provided on the turntable 19, and a plurality of slats 3 can be placed in one station, thereby improving the detection efficiency.

[0067] like Figure 7 and Figure 8 As shown, in one embodiment, the sensing assembly includes a turntable zero position sensor 23 and a turntable working position sensor 24. A boss 25 is provided on the lower surface of the turntable 19 below the slat clamping device 90. The boss 25, the turntable zero position sensor 23 and the turntable working position sensor 24 are located on the same circumference. When the turntable 19 rotates, the lower end of the boss 25 intermittently passes through the turntable zero position sensor 23 and the turntable working position sensor 24. The turntable zero position sensor 23 and the turntable working position sensor 24 record the number of times the turntable 19 rotates in real time, and then convert it into a working position.

[0068] like Figures 10 to 14As shown, in one embodiment, the plate-taking frame module 83 includes a frame 26 for placing slats, a stack 27, a plate-taking frame mechanism and a frame transmission mechanism, the plate-taking frame mechanism is fixed on the stack 27 by a fixing plate 28; the plate-taking frame mechanism includes a first plate-taking support plate 29, a second plate-taking support plate 30 and a second sliding mechanism, the first plate-taking support plate 29 is slidably connected to the second sliding mechanism by a plate-taking connecting plate 31.

[0069] Preferably, a rectangular opening 32 is provided on the first plate retrieval support plate 29, and the second plate retrieval support plate 30 is horizontally slidably arranged at the rectangular opening 32 by means of a screw 33. A spring 34 is provided between the first plate retrieval support plate 29 and the second plate retrieval support plate 30, and an arc-shaped protrusion 35 is provided on the outer side of the second plate retrieval support plate 30, and the protrusion 35 extends outward beyond the outer edge of the first plate retrieval support plate 29; two support ribs 36 are provided on the lower surface of the plate frame 26, and a clamping rib 37 is provided on the inner side of each of the two support ribs 36, wherein an arc-shaped recess 38 is provided on the clamping rib 37 located on one side of the recess 35. When the first plate retrieval support plate 29 and the second plate retrieval support plate 30 enter the stack 27 and extend under the topmost plate frame 26, the first plate retrieval support plate 29 and the second plate retrieval support plate 30 are located between the two clamping ribs 37, and the protrusion 35 sinks into the recess 38. The plate rack taking module 83 smoothly takes the plate rack 26 out of the stack 27 by means of the elastic protrusion 35 clamping the clamping rib 37. When all the slats 3 are transferred to the turntable 19, the plate rack 26 automatically falls into the collection frame and can be used next time.

[0070] like Fig.11 As shown, in one embodiment, the plate rack transmission mechanism includes a lifting motor 39, a plate bracket 40, a screw 41 and two guide rods 42. The two guide rods 42 and the screw 41 are parallel to each other and vertically arranged on one side of the stack 27. The two guide rods 42 and the screw 41 are provided with a lifting slider 43. The plate bracket 40 is fixed on the lifting slider 43 and extends into the stack 27. The plate rack 26 with the slats 3 placed thereon is longitudinally superimposed on the plate bracket 40. The superimposed plate racks 26 are located inside the stack 27. The lower end of the screw 41 is connected to the lifting motor 39 through a lifting motor synchronous belt (not shown in the figure). The plate racks 26 are stacked in the stack 27 in sequence. When the top plate rack 26 is taken out, all the plate racks 26 are raised by one position through the plate rack transmission mechanism. In this way, the plate racks 26 of a column are taken out in sequence, and then the plate racks 26 of a column are manually loaded.

[0071] Preferably, Fig.10As shown, the second sliding mechanism includes a second motor 44 and a second slide rail 45. The plate-taking connecting plate 31 is arranged on the second slide rail 45 and slides along the second slide rail 45. The second motor 44 is connected to the plate-taking connecting plate 31 through a second synchronous belt 46. The second motor 44 rotates to drive the plate-taking connecting plate 31 to slide along the second slide rail 45. The second sliding mechanism can enable the plate-taking frame mechanism to move left and right, thereby taking out the plate frame 26 from the stack 27.

[0072] like Fig.15 , Fig.16 As shown, in one embodiment, the detection module 88 includes an optical path component 47, a slat transfer component, a detection base plate 48 and a third sliding mechanism. A slat drop groove 49 is provided on the detection base plate 48, and the slat transfer component is movably arranged on the upper surface of the detection base plate 48. The optical path component 47 is arranged on the upper surface of the detection base plate 48 through a slat transfer channel 50. When performing detection, the slat 3 on the slat transfer component is located directly below the optical path component.

[0073] like Figures 15 to 17 As shown, the slat transfer assembly includes a slide block 51, a slat plug-in 52 and a DC motor 53. A guide rail 54 is provided on the upper surface of the slide block 51. The slat plug-in 52 is arranged on the guide rail 54. The DC motor 53 is arranged on the slide block 51, and a third gear 55 is provided at the output end. A rack 56 is provided on the slat plug-in 52. The third gear 55 and the rack 56 are meshed with each other. The DC motor 53 rotates and drives the slat plug-in 52 to slide along the guide rail 54. A plurality of plugs 57 arranged parallel to each other are provided on the side of the slat plug-in 52 close to the optical path assembly 47, and the slat 3 is mounted on the plug 57. The slat 3 to be inspected enters the inspection module 88 from one side of the slat transfer channel 50. At this time, the slat plug-in 52 moves toward the slat transfer channel 50, and the insert 57 is inserted into the slat 3 from the side. At this time, the slat plug-in 52 can move the slat 3 left and right or forward and backward. The slat plug-in 52 moves the slat 3 to the bottom of the optical path component 47 for inspection. When the inspection is completed, the DC motor 53 rotates and moves the slat plug-in 52 away from the slat transfer channel 50. When the slat 3 moves to the top of the slat drop groove 49, the slat 3 stops moving due to being blocked by the sliding block 51, and the slat plug-in 52 continues to move away from the slat transfer channel 50, and then the slat plug-in 52 is separated from the slat 3. At this time, the slat 3 falls downward from the slat drop groove 49.

[0074] Preferably, the third sliding mechanism includes a third motor 58 and a third slide rail 59. The slide block 51 is disposed on the third slide rail 59 and slides along the third slide rail 59. The third motor 58 is connected to the slide block 51 through a third synchronous belt 60. The third motor 58 rotates to drive the slide block 51 to slide along the third slide rail 59. The third sliding mechanism enables the slat transfer assembly to move left and right.

[0075] like Figures 1 to 3 As shown, the pushing device 84 includes an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7. The slats on the plate rack module 83 are pushed onto the turntable by the Y-direction pushing mechanism 7, and samples and reagents are added to the reaction cups on the slats 3 on the turntable through the sample adding arm module. The slats on the turntable are pushed into the incubation module 87 by the X-direction pushing mechanism 6.

[0076] like Figure 20 to Figure 23 As shown, in one embodiment, the pushing device 84 also includes a push rod base plate 69, the X-axis pushing mechanism 6 and the Y-axis pushing mechanism 7 of the pushing device 84 have the same structure, and are slidably connected to the push rod base plate 69 through a fourth sliding mechanism and a fifth sliding mechanism respectively, the X-axis pushing mechanism 6 and the Y-axis pushing mechanism 7 both include a push rod 70, a push rod motor 71 and a push rod arm 72, a push rod slide rail 73 is horizontally provided on the push rod arm 72, the push rod 70 is slidably provided on the push rod slide rail 73, and is connected to the push rod motor 71 through a push rod synchronous belt 74.

[0077] Preferably, the fourth sliding mechanism includes a fourth motor 75 and a fourth slide rail 76, the push rod arm 72 is arranged on the fourth slide rail 76, the fourth motor 75 is connected to the push rod arm 72 through a fourth synchronous belt 77, the fourth motor 75 rotates and drives the push rod arm 72 to slide along the fourth slide rail 76, and the fifth sliding mechanism and the fourth sliding mechanism have the same structure.

[0078] like Fig.24 As shown, the sample rack module 86 includes a sample rack base plate 61, a test tube rack 62 and a test tube rack adapter 63. The test tube rack 62 is provided with a plurality of test tube insertion holes 64. The test tube insertion holes 64 are provided with test tube clamping pieces 65. The test tubes 82 for holding samples are inserted into the test tube clamping pieces 65. The sample rack base plate 61 is provided with a plurality of groups of guide blocks 66. The bottom surface of the test tube rack adapter 63 is provided with a guide groove 67. The test tube rack 62 is fixed to the test tube rack adapter 63, and then the test tube rack 62 and the test tube rack adapter 63 are inserted together from one side of the sample rack base plate 61.

[0079] The sample rack module 86 of this structure is convenient for users to operate, and the test tube 82 for placing the sample is relatively stable. The test tube clamping piece 65 provided in the test tube rack can well clamp and fix the test tube containing the sample, thereby ensuring that the test tube is not placed at an angle during the entire detection process, thereby ensuring the reliability and accuracy of sample addition. The setting of the test tube rack adapter can facilitate the insertion and removal of the test tube rack, further ensuring the stability of the test tube.

[0080] like Fig.25 and Fig.26As shown, in one embodiment, one and two magnetic steels 68 are respectively provided on the front end and the lower surface of the test tube rack adapter 63, and the three magnetic steels 68 are all sunk into the test tube rack adapter 63. The magnetic steels 68 can play the role of adsorbing the sample rack bottom plate 61, thereby making the test tube rack adapter 63 more stable.

[0081] In one embodiment, the fully automatic photochemiluminescence detector of the present invention also includes a universal liquid module 78, a liquid circuit module 79 and a dilution and vibration module 80. The sample rack module 86, the reagent module 5, the universal liquid module 78 and the dilution and vibration module 80 realize the operation of adding liquid during the detection process through the liquid circuit module 79.

[0082] In one embodiment, a needle washing pool 81 is provided on one side of the sample rack module 86 and the reagent module 5. The needle washing pool 81 can clean the sample needle on the sample arm module 4, so that the sample needle can be used multiple times.

[0083] The detection method of the fully automatic photoluminescence detector is introduced in detail below:

[0084] The space occupied by the turntable 19 (fixed position, does not rotate with the rotation of the turntable 19) is divided into a D0 area, a D1 area, a D2 area and a D3 area arranged in sequence (the D0 area, the D1 area and the D3 area are as follows Fig. 20 As shown in the figure, the D2 area is not shown because it is covered by the pushing device 84, wherein the D0 area is used to control the plate rack module and the pushing device 84 to load the blank slats onto the turntable module 85, and the D1, D2 and D3 areas are used to control the turntable module 85 and the sample loading arm module 4 to add the solution containing the sample to be tested and the reaction reagent to the blank slats. In addition, the D1 area is also used to complete the operation of adding the diluted sample. The D3 area is used to complete the operation of adding the reaction reagent and unloading. Four slat clamping devices 90 are set on the turntable 19 to clamp the blank slats in the horizontal direction and the vertical direction.

[0085] In this embodiment, the first pushing mechanism in the pushing device 84 is the Y-direction pushing mechanism 7, the second pushing mechanism in the moving mechanism is the X-direction pushing mechanism 6, the first preset direction is the Y direction, and the second preset direction is the X direction.

[0086] When the detection program is started, the plate rack mechanism in the plate rack module 83 first takes out the plate rack 26 carrying the blank slats from the stack 27 in the plate rack module 83, and then controls the Y-axis pushing mechanism 7 to drive the blank slats on the plate rack 26 to move in the Y direction, so that the blank slats are moved to the position corresponding to the D0 area of ​​the turntable 19, and are clamped by the slat clamping device 90 to make the slats more stable during the movement.

[0087] In the plate rack taking module 83, the plate racks 26 are stacked in the stack 27 in sequence. When the plate rack taking mechanism takes out a layer of plate racks 26 from the top of the stack 27, the plate rack transmission mechanism drives the stack 27 to rise by one position, that is, the height between adjacent plate racks 26. In this way, the plate racks 26 of a column are taken out in sequence, and then the plate racks 26 of a column are manually loaded.

[0088] In this embodiment, the sample adding mechanism is the left arm of the sample adding arm module 4, and the reagent adding mechanism is the right arm of the sample adding arm module 4. After the solution containing the sample to be tested is added to the left arm of the sample adding arm, it can be cleaned by the first needle washing pool in the needle washing pool 81, and after the reaction reagent is added to the right arm of the sample adding arm, it can be cleaned by the second needle washing pool in the needle washing pool 81.

[0089] After the blank slat is clamped on the turntable 19 by the slat clamping device 90, specifically, the turntable 19 is controlled to rotate so that the blank slat reaches the D1 area; the left arm of the sample loading arm is controlled to add the solution containing the sample to be tested to the blank slat; the turntable 19 is controlled to rotate so that the slat to which the solution containing the sample to be tested is added reaches the D2 area; the turntable 19 is controlled to rotate so that the slat to which the solution containing the sample to be tested is added reaches the D3 area; the right arm of the sample loading arm is controlled to add the reaction reagent to the slat to which the solution containing the sample to be tested is added. When the blank slat reaches the D1 area, the right arm of the sample loading arm is controlled to add the additional reaction reagent to the blank slat in the D1 area. This embodiment does not limit the order of adding the solution containing the sample to be tested and adding the additional reaction reagent. Preferably, the additional reaction reagent needs to be added before the sample is distributed. Specifically, when the blank slat reaches the D1 area, the right arm of the sample loading arm is controlled to add the additional reaction reagent to the blank slat in the D1 area, and then the left arm of the sample loading arm is controlled to add the sample to the blank slat.

[0090] In a preferred embodiment, the solution containing the sample to be tested is also diluted before being added to the blank strip. When the blank strip reaches the D1 area, the right arm of the sample loading arm is controlled to add the pre-diluted solution to the pre-dilution plate in the dilution oscillation module 80; the left arm of the sample loading arm is controlled to add the solution containing the sample to be tested to the pre-dilution plate in the dilution oscillation module 80; the dilution oscillation module 80 is controlled to oscillate the pre-dilution plate to obtain the diluted sample; the left arm of the sample loading arm is controlled to add the diluted sample to the blank strip in the D1 area. More preferably, the diluted sample can be added to a part of the sample area of ​​the blank strip in the D1 area.

[0091] Specifically, the sample adding process can adopt a combined sample adding method of one aspiration and multiple distribution. For example, if n projects need to be done, only one of which needs to be pre-diluted, the sample adding mechanism aspirates n samples, only distributes one sample to the pre-dilution plate, and distributes the other n-1 samples to the blank strips in the D1 area. After the dilution of one sample in the pre-dilution plate is completed, the left arm of the robot arm is controlled to add the diluted sample from the pre-dilution plate to the blank strips in the D1 area.

[0092] When the slat reaches the D3 area, the right arm of the robot is controlled to add one or more reaction reagents to the slat.

[0093] The reaction reagents added to the strips are all aqueous solutions.

[0094] In this embodiment, the unloading mechanism is controlled to unload the slats carrying the mixed solution containing the solution of the sample to be tested and the reaction reagent from the turntable module 85. Figure 2 After the solution containing the sample to be tested and the reaction reagent are mixed, the unloading mechanism is controlled to unload the slats on the turntable 19 from the slat clamping device 90 .

[0095] Afterwards, the push device 84 is controlled to move the unloaded slats to the incubation module. Figure 2 as well as Fig. 20 , control the X-direction pushing mechanism 6 to drive the slats carrying the mixed solution to move along the X direction, so that the slats carrying the mixed solution move to the incubation module 87.

[0096] Afterwards, the incubation module 87 is controlled to incubate the mixed liquid on the unloaded slats. When incubating the slats carrying the mixed liquid, the first sliding mechanism in the incubation module 87 is controlled to drive the unloaded slats to slide back and forth to mix the mixed liquid on the slats; during the mixing process, the incubation plate 8 in the incubation module 87 is controlled to incubate the mixed liquid.

[0097] After the incubation is completed, the moving arm in the push device 84 is controlled to move the slat carrying the incubated mixed solution to the detection module 88. When the slat carrying the incubated mixed solution enters the detection module 88, the slat transfer assembly in the detection module 88 is controlled to drive the slat carrying the incubated mixed solution to move to the bottom of the optical path assembly 47 in the detection module 88, and the optical path assembly 47 is controlled to perform laser irradiation on the incubated mixed solution.

[0098] The detection module is then controlled to irradiate the incubated mixed solution with a laser and record the amount of emitted light.

[0099] In one embodiment, there are multiple incubation modules 87, and multiple incubation modules 87 are used to incubate the mixed solution on the unloaded slats twice, and it is determined whether the high-dose-hook effect is detected. First, the first incubation module is controlled to perform the first incubation on the mixed solution; the slat carrying the mixed solution after the first incubation is moved to the detection module; the detection module 88 is controlled to perform the first laser irradiation on the mixed solution after the first incubation and record the amount of emitted light; the slat carrying the mixed solution after the first reading is moved to the second incubation module; the second incubation module is controlled to perform the second incubation on the mixed solution after the first reading; the slat carrying the mixed solution after the second incubation is moved to the detection module 88; the detection module 88 is controlled to perform the second laser irradiation on the mixed solution after the second incubation and record the amount of emitted light. During the first incubation process, the right arm of the sample loading arm is controlled to absorb the universal liquid from the universal liquid module 78 and add it to the mixed solution being incubated.

[0100] The control processor determines whether there is a high-dose-hook effect based on the amount of emitted light recorded after two incubations. Wherein, the control processor determines whether there is a high-dose-hook effect based on the amount of emitted light recorded after two incubations, including: calculating the difference between the amount of emitted light recorded after the first incubation and the amount of emitted light recorded after the second incubation; judging whether the difference is greater than a preset threshold; if it is judged that the difference is greater than the preset threshold, it is determined that there is a high-dose-hook effect; otherwise, if it is judged that the difference is less than or equal to the preset threshold, it is determined that there is no high-dose-hook effect. Here, the preset threshold is the maximum value of the standard curve, and the standard curve is measured when the sample to be tested is a standard substance, and the concentration of the standard substance is lower than the concentration when the high-dose-hook effect exists. In a preferred embodiment, when the processor determines that there is a high-dose-hook effect in the immunoassay, the dilution and oscillation module 80 is controlled to dilute the current mixed solution until there is no high-dose-hook effect.

[0101] In one embodiment, the slats 3 used for detection are placed side by side on the plate rack 26, and multiple plate racks 26 are stacked in the stack 27 of the plate rack retrieval module 83. The top plate rack 26 is taken out by the plate rack retrieval mechanism, and then the slats 3 are pushed to the slat clamping device on the turntable 19 by the Y-axis pushing mechanism 7. The turntable 19 rotates 90 degrees clockwise, and the slats 3 reach the sample adding position for sample adding and dilution. At this time, a slat 3 is pushed from the plate rack 26 to the slat clamping device again, and then rotated 90 degrees clockwise. A slat 3 is pushed at the pushing position for every 90 degrees rotation, and then the sample is added at the sample adding position. After rotating 90 degrees twice, it reaches the reagent adding position for reagent adding operation, and then the slats 3 are pushed into the incubation module 87 by the X-axis pushing mechanism 6 for incubation, and then enter the detection module 88 for detection.

[0102] The plate rack module 83 of the present invention facilitates the plate rack 26 to be taken out and sent to the turntable 19 in an orderly manner. The turntable 19 can efficiently complete operations such as adding samples, adding reagents, and diluting, which greatly reduces the volume of the entire instrument and makes the detection process more efficient. Its pushing device 84 includes an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7. The slats on the plate rack module 83 are pushed to the turntable by the Y-direction pushing mechanism 7. The sample and reagent are added to the reaction cup on the slats 3 on the turntable through the sample adding arm module. The slats on the turntable are pushed into the incubation module 87 by the X-direction pushing mechanism 6, which can ensure the accuracy and safety of the slats during the transportation process. The incubation module 87 includes an incubation plate 8 and a first sliding mechanism. The slats 3 are fixed on the incubation plate 8, and then slide back and forth through the first sliding mechanism to perform oscillation mixing, and perform incubation treatment at the same time, so that the detection efficiency is higher, and then enter the detection module 88 for detection. The sample rack module 86 can conveniently and stably place the sample in the sample area, and the detection module 88 can conveniently discard the slats 3 after detection, which is fast and flexible, thereby ensuring the high efficiency of the detection.

[0103] Although the present invention has been described with reference to preferred embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, the various technical features mentioned in the various embodiments may be combined in any manner as long as there is no conflict. The present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A fully automatic photoluminescence detector, characterized in that: It comprises a plate taking rack module, a pushing device, a sample loading arm module, a turntable module, a sample rack module, an incubation module, a reagent module and a detection module, wherein the turntable module comprises a turntable base and a disk body assembly, the disk body assembly comprises a turntable, a plurality of slat clamping devices are arranged on the turntable, the slat clamping devices can hold a plurality of slats, the slats on the plate taking rack module are pushed to the loading area of ​​the turntable module by the Y-direction pushing mechanism of the pushing device, the sample and the reagent are added to the reaction cup on the slat located in the sample loading area of ​​the turntable module by the sample loading arm module, the X-direction pushing mechanism of the pushing device drives the slat carrying the mixed solution on the turntable module to move to the incubation module, and enters the detection module for detection after the incubation is completed; The board-taking rack module comprises a board rack for placing the slats; When the turntable rotates 90 degrees clockwise, the slat reaches the sample loading position for loading and dilution. At this time, push another slat from the plate rack to the slat clamping device, and then rotate 90 degrees clockwise. Push a slat at the pushing position for every 90 degrees of rotation, then add the sample at the sample loading position, and then rotate 90 degrees twice before reaching the reagent adding position for reagent adding.

2. The fully automatic photochemiluminescence detector according to claim 1, characterized in that: The turntable module also includes a shaft assembly, the turntable base is fixed on the frame, the disk body assembly also includes a first gear and a gear pressure plate, four slat clamping devices are arranged symmetrically on the same circumference on the upper surface of the turntable, the shaft assembly passes through the entire gear pressure plate and the first gear from bottom to top, the upper part of the shaft assembly is fixed on the lower surface of the turntable, and a second gear is provided on the output end of the turntable motor, and the second gear is meshed with the first gear.

3. The fully automatic photochemiluminescence detector according to claim 2, characterized in that: A boss is provided on the lower surface of the turntable below the slat clamping device, and the boss is located on the same circumference as the turntable zero position sensor and the turntable working position sensor. When the turntable rotates, the lower end of the boss intermittently passes through the turntable zero position sensor and the turntable working position sensor.

4. The fully automatic photoluminescence detector according to claim 1, characterized in that: The plate retrieval rack module includes a plate rack for placing slats, a stack, a plate retrieval rack mechanism and a plate rack transmission mechanism, and the plate retrieval rack mechanism is fixed to the stack through a fixed plate; the plate retrieval rack mechanism includes a first plate retrieval support plate, a second plate retrieval support plate and a second sliding mechanism, and the first plate retrieval support plate is slidably connected to the second sliding mechanism through a plate retrieval connecting plate.

5. The fully automatic photochemiluminescence detector according to claim 4, characterized in that: A rectangular opening is provided on the first plate-taking support plate, and the second plate-taking support plate is horizontally slidably arranged at the rectangular opening by screws. A spring is provided between the first plate-taking support plate and the second plate-taking support plate, and an arc-shaped protrusion is provided on the outer side of the second plate-taking support plate, and the protrusion extends outward beyond the outer edge of the first plate-taking support plate; two supporting ribs are provided on the lower surface of the plate rack, and a clamping rib is provided on the inner side of each of the two supporting ribs, wherein an arc-shaped depression is provided on the clamping rib on one side of the protrusion, and when the first plate-taking support plate and the second plate-taking support plate enter the stack and extend under the topmost plate rack, the first plate-taking support plate and the second plate-taking support plate are located between the two clamping ribs, and the protrusion sinks into the depression.

6. The fully automatic photochemiluminescence detector according to claim 1, characterized in that: The detection module includes an optical path component, a slat transfer component, a detection base plate and a third sliding mechanism. The detection base plate is provided with a slat drop groove, and the slat transfer component is movably arranged on the upper surface of the detection base plate. The optical path component is arranged on the upper surface of the detection base plate through a slat transfer channel. When performing detection, the slats on the slat transfer component are located directly below the optical path component.

7. The fully automatic photochemiluminescence detector according to claim 6, characterized in that: The slat transfer assembly includes a sliding block, a slat plug-in and a DC motor. A guide rail is provided on the upper surface of the sliding block, the slat plug-in is arranged on the guide rail, the DC motor is arranged on the sliding block, and a third gear is provided at the output end. A rack is provided on the slat plug-in, and the third gear and the rack are meshed with each other. The DC motor rotates to drive the slat plug-in to slide along the guide rail. A plurality of plugs arranged parallel to each other are provided on the side of the slat plug-in close to the optical path assembly, and the slat is mounted on the plugs.

8. The fully automatic photochemiluminescence detector according to claim 7, characterized in that: The third sliding mechanism includes a third motor and a third slide rail. The slide block is arranged on the third slide rail and slides along the third slide rail. The third motor is connected to the slide block through a third synchronous belt. The third motor rotates to drive the slide block to slide along the third slide rail.

9. The fully automatic photochemiluminescence detector according to claim 1, characterized in that: The pushing device also includes a push rod base plate. The X-direction pushing mechanism and the Y-direction pushing mechanism of the pushing device have the same structure and are slidably connected to the push rod base plate through a fourth sliding mechanism and a fifth sliding mechanism respectively. The X-direction pushing mechanism and the Y-direction pushing mechanism both include a push rod, a push rod motor and a push rod arm. A push rod slide rail is horizontally provided on the push rod arm. The push rod is slidably arranged on the push rod slide rail and is connected to the push rod motor through a push rod synchronous belt.

10. The fully automatic photochemiluminescence detector according to claim 9, characterized in that: The fourth sliding mechanism includes a fourth motor and a fourth slide rail, the push rod arm is arranged on the fourth slide rail, the fourth motor is connected to the push rod arm through a fourth synchronous belt, the fourth motor rotates to drive the push rod arm to slide along the fourth slide rail, and the fifth sliding mechanism and the fourth sliding mechanism have the same structure.

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