A multi-light path optical machine imaging device

Abstract

The invention discloses a multi-light path optical machine imaging device, including a base and a plurality of fluorescence microscopic imaging devices; a bonding portion is provided for adjacent two of a camera component, an illumination component and a focusing component in the same fluorescence microscopic imaging device; the bonding portion includes a plurality of mortise and tenon joint surfaces that are matched, nested and fixed by gluing; any pair of mutually gluing mortise and tenon joint surfaces in the same bonding portion are distributed opposite to and not perpendicular to at least one pair of another mutually gluing mortise and tenon joint surfaces; more than three base bonding portions are provided between any fluorescence microscopic imaging device and the base; the bonding claw I and the bonding groove I of any base bonding portion are fixed by adhesive I, and the bonding claw II and the bonding groove II are fixed by adhesive II. The device ensures the assembly accuracy of a single fluorescence microscopic imaging device and limits its volume through the bonding portion, and integrates a plurality of fluorescence microscopic imaging devices precisely into the base in combination with the base bonding portion, so that the observation object is observed simultaneously by using a plurality of imaging light paths to improve the observation efficiency.

Description

Technical Field

[0001] The present invention relates to the field of optical detection, and in particular to a multi-light path optical machine imaging device. Background Art

[0002] Optical imaging equipment is a powerful tool for observing and studying samples, with sub-micron resolution. In the field of biological sample detection, some optically detectable groups / compounds / structures are usually used to mark the biological molecules to be detected, and then optical imaging equipment is used to collect signals from these markers to achieve the location or detection of the biological molecules to be detected.

[0003] Optical imaging equipment can adopt the principle of fluorescence microscopy, which includes various types of optical and optoelectronic elements, such as industrial cameras, tube lenses, objectives, filters of various bandwidths, apertures of various sizes, lenses, focus drive devices (such as voice coil motors or ceramic motors), LD light sources, LED light sources, etc.

[0004] Since optical imaging equipment has very high requirements for the assembly accuracy of some optical and optoelectronic components, it is generally necessary to add a position adjustment compensation device to the product to ensure the actual assembly position of the aforementioned optical and optoelectronic components. This results in a larger product size, which is not only inconvenient to use and difficult to maintain, but also has low observation efficiency, increasing the workload of operators. Summary of the invention

[0005] The purpose of the present invention is to provide a multi-light path optical mechanical imaging device, which can improve observation efficiency, reduce operation frequency and equipment maintenance frequency, thereby ensuring the operating effect of operators and reducing the operating burden.

[0006] To achieve the above-mentioned purpose, the present invention provides a multi-light path optical machine imaging device, comprising a base and a plurality of fluorescence microscopic imaging devices positioned and installed on the base; any of the fluorescence microscopic imaging devices has an imaging light path, and all of the imaging light paths of all of the fluorescence microscopic imaging devices are distributed at intervals; any of the fluorescence microscopic imaging devices comprises a camera assembly having an industrial camera, an illumination assembly connected to a tube lens, and a focusing assembly having a voice coil motor; the camera assembly, the illumination assembly, and the focusing assembly are interconnected, and a bonding portion is provided between two adjacent ones; any of the bonding portions comprises a plurality of mortise and tenon joint surfaces that are mutually matched, nested, and fixed by gluing; any pair of mutually gluing mortise and tenon joint surfaces in the same bonding portion are distributed opposite to and not perpendicular to at least one pair of another mutually gluing mortise and tenon joint surfaces;

[0007] More than three non-collinear base bonding parts are provided between any of the fluorescence microscopy imaging devices and the base; any of the base bonding parts includes bonding claws I, bonding grooves I, bonding claws II and bonding grooves II; the bonding claws I and the bonding grooves I are respectively provided at the base and the fluorescence microscopy imaging device and are bonded and fixed with adhesive I; the bonding claws II and the bonding grooves II are respectively provided at the base and the fluorescence microscopy imaging device and are bonded and fixed with adhesive II; the curing rate of the adhesive I is greater than the curing rate of the adhesive II, and the bonding strength of the adhesive II is greater than the bonding strength of the adhesive I.

[0008] Preferably, the bonding groove I is a through groove that passes through from top to bottom; the bonding groove II is a sink groove; and the adhesive I is specifically a UV-curing optical adhesive.

[0009] Preferably, the bonding claw II is inserted into the sink from top to bottom, and the bonding claw II gradually shrinks from the middle to the top in the height direction; the top of the bonding claw I is different in shape from the top of the bonding claw II.

[0010] Preferably, all the bonding parts include a first bonding part provided between the camera assembly and the lighting assembly and a second bonding part provided between the focusing assembly and the lighting assembly;

[0011] A normal line of any of the mortise and tenon joint surfaces in the first bonding portion is perpendicular or parallel to a first joint direction of the camera assembly and the lighting assembly;

[0012] A normal line of any of the mortise and tenon joint surfaces in the second bonding portion is perpendicular or parallel to a second joint direction of the focusing assembly and the lighting assembly.

[0013] Preferably, the normal of any mortise and tenon jointing surface in the first bonding portion is perpendicular to the first splicing direction; all the mortise and tenon jointing surfaces in the first bonding portion include flat adhesive surfaces and oblique adhesive surfaces; the flat adhesive surface is parallel to the first reference direction or the second reference direction; the oblique adhesive surface is not parallel to the first reference direction, and the oblique adhesive surface is not parallel to the second reference direction; wherein any two of the first reference direction, the second reference direction and the first splicing direction are perpendicular to each other.

[0014] Preferably, the normal of any mortise and tenon joint surface in the second bonding portion is parallel to the second splicing direction; all the mortise and tenon joint surfaces in the second bonding portion are spaced apart in multiple layers along the second splicing direction, and the projections of all the mortise and tenon joint surfaces overlap in a reference plane; and the reference plane is perpendicular to the second splicing direction.

[0015] Preferably, the end of the voice coil motor and the end of the lighting assembly are both provided with a tenon portion and a mortise portion in the shape of chain teeth.

[0016] Preferably, the lighting assembly comprises a lighting element, a lens sleeve, a main support, and a plurality of shaft sets disposed between the lens sleeve and the main support;

[0017] The lighting element is arranged above the lens in the lens sleeve;

[0018] The main support is provided with a mounting cavity for accommodating and upwardly supporting the lens sleeve and a plurality of adjustment cavities for one-to-one correspondence between all the shaft sleeves to achieve axial movement and positioning; two pairs of mounting surfaces are provided on the circumferential side of the lens sleeve; any one of the mounting surfaces abuts against the shaft sleeve; at least one of the mounting surfaces abuts against two or more shaft sleeves; all the shaft sleeves abutting against the same pair of mounting surfaces move in the same direction, and the movement directions of the shaft sleeves abutting against the two pairs of mounting surfaces are perpendicular to each other and are perpendicular to the vertical direction.

[0019] Preferably, the entire shaft kit includes two spring plungers and multiple rigid ball plungers with external threads on the circumferential side; one of any pair of mounting surfaces abuts against one of the spring plungers and the other abuts against the rigid ball plunger; the adjustment cavity that cooperates with the axial hole of the rigid ball plunger is specifically a threaded hole.

[0020] Preferably, it further comprises a rigid sleeve positioning member for moving in the vertical direction; one axial end of the rigid sleeve positioning member is relatively fixed to the lens sleeve.

[0021] Compared with the above-mentioned background technology, the multi-light-path optical mechanical imaging equipment provided by the present invention includes a base and a plurality of fluorescence microscopy imaging devices positioned and installed on the base; wherein, more than three non-collinear base bonding parts are provided between any one of the fluorescence microscopy imaging devices and the base; and any one of the fluorescence microscopy imaging devices includes a camera assembly having an industrial camera, an illumination assembly arranged on a tube lens, and a focusing assembly having a voice coil motor.

[0022] In the multi-light-path optical-mechanical imaging device, any one of the fluorescence microscopic imaging devices has an imaging light path, and the imaging light paths of all the fluorescence microscopic imaging devices are distributed at intervals, so as to simultaneously collect images of multiple observation points of the observed object.

[0023] In the multi-light path optical machine imaging device, the camera assembly, the lighting assembly and the focusing assembly of any one fluorescence microscopic imaging device are interconnected and a bonding portion is provided between two adjacent ones. Among them, any one bonding portion includes a plurality of mortise and tenon joint surfaces that are mutually matched, nested and glued and fixed; in the same bonding portion, any pair of mutually glued mortise and tenon joint surfaces are distributed opposite to at least one pair of another mutually glued mortise and tenon joint surfaces and are not perpendicular.

[0024] In the multi-light path optical imaging device, any one of the base bonding parts includes bonding claws I, bonding grooves I, bonding claws II and bonding grooves II; wherein, bonding claws I and bonding grooves I are respectively provided on the base and the fluorescence microscopic imaging device and are bonded and fixed by adhesive I; bonding claws II and bonding grooves II are respectively provided on the base and the fluorescence microscopic imaging device and are bonded and fixed by adhesive II. The curing rate of the aforementioned adhesive I is greater than the curing rate of the aforementioned adhesive II, and the bonding strength of the aforementioned adhesive II is greater than the bonding strength of the aforementioned adhesive I.

[0025] For the above-mentioned multi-light path optical machine imaging equipment, the assembly accuracy of the important parts of a single fluorescence microscopy imaging device can be guaranteed by the bonding part, so there is no need to add a position adjustment compensation device. Therefore, the assembled single fluorescence microscopy imaging device has a compact structure, small size, and regular and complete appearance, which is conducive to the rapid integration and installation of multiple fluorescence microscopy imaging devices on the base, and at the same time, it is convenient to accurately control the relative position accuracy of all fluorescence microscopy imaging devices on the base. A single fluorescence microscopy imaging device and the base are glued and fixed using more than three different lines of the base bonding parts. The adhesive I used in the base bonding part has a fast curing speed, which can realize the rapid positioning of the fluorescence microscopy imaging device and the base. The adhesive II used in the base bonding part has a high curing strength. Under the pre-positioning effect of the adhesive I, the fluorescence microscopy imaging device and the base are both assembled with assembly accuracy and assembly strength.

[0026] In summary, the above-mentioned multi-optical path optical mechanical imaging equipment utilizes all the imaging optical paths of all the integrated fluorescence microscopy imaging devices to simultaneously observe multiple parts of the observation object, thereby exponentially improving the observation efficiency, reducing the operator's operating frequency and equipment maintenance frequency to reduce the operator's workload and improve operating efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0028] Figure 1 A schematic diagram of the structure of a multi-light-path optical-mechanical imaging device provided by an embodiment of the present invention;

[0029] Figure 2 for Figure 1 Exploded view of;

[0030] Figure 3 A schematic diagram of the structure of a fluorescence microscopy imaging device provided in an embodiment of the present invention;

[0031] Figure 4 A schematic diagram of the assembly structure of the fluorescence microscopic imaging device, the multi-degree-of-freedom adjustment device connector, and the detection device connector provided in an embodiment of the present invention;

[0032] Figure 5 A schematic diagram of assembling a camera bracket and a tube lens bracket in a first direction in a fluorescence microscopy imaging device provided by an embodiment of the present invention;

[0033] Figure 6 A schematic diagram of assembling a camera bracket and a tube lens bracket in a second direction in a fluorescence microscopic imaging device provided by an embodiment of the present invention;

[0034] Figure 7 for Figure 6 Cross-sectional view along AA direction;

[0035] Figure 8 A schematic diagram of assembling an illumination assembly and a voice coil motor assembly in a fluorescence microscopy imaging device provided by an embodiment of the present invention in a third direction;

[0036] Fig. 9 A schematic diagram of assembling an illumination assembly and a voice coil motor assembly in a fluorescence microscopy imaging device provided by an embodiment of the present invention in a fourth direction;

[0037] Fig.10 A schematic diagram of the structure of a lighting assembly provided by an embodiment of the present invention;

[0038] Fig.11 A schematic diagram of the local structure of the lens sleeve of the lighting assembly provided by an embodiment of the present invention;

[0039] Fig.12 A schematic diagram of the structure of the base bonding portion provided by an embodiment of the present invention;

[0040] Fig.13 A schematic diagram of the structure of a microscopic imaging unit provided by an embodiment of the present invention;

[0041] Fig.14 A cross-sectional view of a base bonding portion provided by an embodiment of the present invention.

[0042] in,

[0043] 01-connector of multi-degree-of-freedom adjustment device, 02-connector of detection device, 03-transparent tape;

[0044] 1-support plate, 2-crossbeam, 4-industrial camera, 5-camera bracket, 6-dustproof ring, 7-tube lens, 8-tube lens bracket, 10-objective lens seat, 11-objective lens, 12-voice coil motor, 13-focus light source assembly;

[0045] 9-lighting assembly, 91-heat sink, 92-guide column, 93-LED, 94-lens, 95-lens sleeve, 96-aperture, 97-adjusting column, 98-main support, 99-axis kit, 910-first nut, 911-second nut, 9121-first spring plunger, 9122-second spring plunger, 9123-first ball plunger, 9124-second ball plunger, 9125-third ball plunger, 913-rigid sleeve locator, 914-mounting surface, 9141-first mounting surface, 9142-second mounting surface, 9143-third mounting surface, 915-guide column locator;

[0046] 20-mortise and tenon joint surface, 201-flat adhesive surface, 202-oblique adhesive surface;

[0047] 321-microscope imaging part, 322-base part, 3221-first base part, 3222-second base part, 3201-adhesive claw II, 3202-adhesive claw II-I, 3203-adhesive claw I-II, 3204-adhesive claw II-II, 3205-adhesive claw I-III, 3206-adhesive claw II-III. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] In order to enable those skilled in the art to better understand the scheme of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0050] Please refer to Figures 1 to 14 , Figure 1 A schematic diagram of the structure of a multi-light-path optical-mechanical imaging device provided by an embodiment of the present invention; Figure 2 for Figure 1 Exploded view of; Figure 3 A schematic diagram of the structure of a fluorescence microscopy imaging device provided in an embodiment of the present invention; Figure 4 A schematic diagram of the assembly structure of the fluorescence microscopic imaging device, the multi-degree-of-freedom adjustment device connector, and the detection device connector provided in an embodiment of the present invention; Figure 5 A schematic diagram of assembling a camera bracket and a tube lens bracket in a first direction in a fluorescence microscopy imaging device provided by an embodiment of the present invention; Figure 6 A schematic diagram of the assembly of a camera bracket and a tube lens bracket in a second direction in a fluorescence microscopic imaging device provided by an embodiment of the present invention; Figure 7for Figure 6 Cross-sectional view along AA direction; Figure 8 A schematic diagram of assembling an illumination assembly and a voice coil motor assembly in a fluorescence microscopy imaging device provided by an embodiment of the present invention in a third direction; Fig. 9 A schematic diagram of assembling an illumination assembly and a voice coil motor assembly in a fluorescence microscopy imaging device provided by an embodiment of the present invention in a fourth direction; Fig.10 A schematic diagram of the structure of a lighting assembly provided by an embodiment of the present invention; Fig.11 A schematic diagram of the local structure of the lens sleeve of the lighting assembly provided by an embodiment of the present invention; Fig.12 A schematic diagram of the structure of the base bonding portion provided by an embodiment of the present invention; Fig.13 A schematic diagram of the structure of a microscopic imaging unit provided by an embodiment of the present invention; Fig.14 This is a cross-sectional view of the base bonding portion provided by an embodiment of the present invention. Figure 7 and Fig.10 The coordinate systems of the two are independent of each other.

[0051] The present invention provides a multi-light path optical machine imaging device, comprising a base and a plurality of fluorescence microscopic imaging devices positioned and mounted on the base; any one of the fluorescence microscopic imaging devices comprises a camera assembly having an industrial camera 4, an illumination assembly 9 fixedly connected with a tube lens 7, and a focusing assembly having a voice coil motor 12; any one of the fluorescence microscopic imaging devices has an imaging light path, and all imaging light paths of all the fluorescence microscopic imaging devices are distributed at intervals. In addition, three or more non-collinear base bonding portions are provided between any one of the fluorescence microscopic imaging devices and the base.

[0052] For a single fluorescence microscopy imaging device, the camera assembly, the lighting assembly 9 and the focusing assembly are interconnected and a bonding portion is provided between any two adjacent ones. For example, the camera assembly, the lighting assembly 9 and the focusing assembly are connected in sequence, then a bonding portion is provided between the camera assembly and the lighting assembly 9, and a bonding portion is provided between the lighting assembly 9 and the focusing assembly.

[0053] Any one bonding portion includes a plurality of mutually fitting, nested and glued mortise and tenon joint surfaces 20; any pair of mutually glued mortise and tenon joint surfaces 20 in the same bonding portion are distributed opposite to and not perpendicular to at least one other pair of mutually glued mortise and tenon joint surfaces 20.

[0054] Taking the bonding portion between the camera assembly and the lighting assembly 9 as an example, the end of the camera assembly may be provided with a plurality of protrusions distributed at intervals, and the aforementioned plurality of protrusions enable the end of the camera assembly to form a plug-in port having both a mortise and a tenon; the end of the lighting assembly 9 is provided with a plurality of protrusions distributed at intervals, and the aforementioned plurality of protrusions enable the end of the lighting assembly 9 to form a plug-in port having both a mortise and a tenon. The aforementioned plug-in port of the camera assembly and the aforementioned plug-in port of the lighting assembly 9 may be matched and nested with each other and fixed by gluing, so the mortise and tenon joint surface 20 is used to define the partial surface between the camera assembly and the lighting assembly 9 that is fixed by gluing.

[0055] Still taking the bonding portion provided between the camera assembly and the lighting assembly 9 as an example, the aforementioned bonding portion includes a first surface and a second surface provided at the end of the camera assembly and a third surface and a fourth surface provided at the end of the lighting assembly 9. The aforementioned first surface, second surface, third surface and fourth surface fit in pairs when the plug-in port of the camera assembly and the plug-in port of the lighting assembly 9 are plugged into each other, specifically, the first surface and the third surface fit in pairs and are glued and fixed, and the second surface and the fourth surface fit in pairs and are glued and fixed. For the aforementioned structure, the first surface, the third surface and the glue layer therebetween can be regarded as one plane, and the second surface, the fourth surface and the glue layer therebetween can be regarded as another plane, and the aforementioned two planes are distributed facing each other, for example, they can be parallel or inclined to each other, but not perpendicular. In this way, the shrinkage of multiple adhesive layers located in different planes during the curing process and the expansion and contraction of the adhesive layers due to temperature changes during the use of the product can offset and compensate each other, thereby effectively overcoming the position drift of the camera assembly and the lighting assembly 9 caused by the expansion and contraction of the colloid. On the one hand, it effectively improves the assembly accuracy of the camera assembly and the lighting assembly 9 during the assembly process, and on the other hand, it weakens the interference of temperature on the relative position relationship of the camera assembly and the lighting assembly 9 during use, thereby ensuring the long-term position assembly accuracy of the camera assembly and the lighting assembly 9.

[0056] Obviously, the more pairs of mortise and tenon joints 20 there are between the camera assembly and the lighting assembly 9, and the different angles of different pairs of mortise and tenon joints 20, the greater the probability that the compensation for the position drift caused by the expansion and contraction of the colloid by multiple mortise and tenon joints 20 will be close to the ideal value, that is, the better the compensation effect. Of course, as the number of mortise and tenon joints 20 between the camera assembly and the lighting assembly 9 increases, the difficulty of processing and assembling the camera assembly and the lighting assembly 9 also increases. Therefore, the number and angle of the mortise and tenon joints 20 between the camera assembly and the lighting assembly 9 can be designed according to the actual needs of different products for the camera assembly and the lighting assembly 9.

[0057] Similarly, for the lighting component 9 and the focusing component, the mortise and tenon joint surface 20 is defined in this article as the partial surface between the lighting component 9 and the focusing component that is glued and fixed. The specific structure of the mortise and tenon joint surface 20 between the lighting component 9 and the focusing component can be referred to the above record.

[0058] In a single fluorescence microscopy imaging device, since the assembly accuracy of the camera assembly, the lighting assembly 9 and the focusing assembly is ensured by the bonding portion, there is no need to add a position adjustment compensation device between the camera assembly, the lighting assembly 9 and the focusing assembly for adjusting the positions of the components during the assembly process, which further results in the assembled single fluorescence microscopy imaging device having a compact structure, a small size, and a regular and complete appearance, which is conducive to the integrated installation of multiple fluorescence microscopy imaging devices on the base and facilitates the precise control of the relative position accuracy of all fluorescence microscopy imaging devices on the base.

[0059] In addition, in the multi-light path optical imaging device provided by the present invention, a single fluorescence microscopy imaging device is glued and fixed to the base using more than three base adhesive portions, and among all the base adhesive portions between the same fluorescence microscopy imaging device and the base, at least three base adhesive portions are not collinear.

[0060] For any one of the base bonding parts, it includes bonding claws I, bonding grooves I, bonding claws II and bonding grooves II. The bonding claws I and bonding grooves I of the base bonding part are respectively arranged on the base and the fluorescence microscopy imaging device, and the bonding claws I and bonding grooves I are both bonded and fixed by adhesive I; the bonding claws II and bonding grooves II of the base bonding part are respectively arranged on the base and the fluorescence microscopy imaging device, and the bonding claws I and bonding grooves I are both bonded and fixed by adhesive II. Among them, the curing rate of the aforementioned adhesive I is greater than the curing rate of the aforementioned adhesive II, and the bonding strength of the aforementioned adhesive II is greater than the bonding strength of the aforementioned adhesive I.

[0061] Any of the above-mentioned base bonding parts plays a role in gluing and fixing the fluorescence microscopy imaging device and the base. All the base bonding parts between the same fluorescence microscopy imaging device and the base are beneficial to improving the force characteristics of the base bonding parts to increase the gluing and fixing strength. For example, the torque effect formed by too few bonding points and the excessive weight of the fluorescence microscopy imaging device can be overcome; the adhesive I used in any base bonding part has a fast curing speed, which can realize the rapid positioning of the fluorescence microscopy imaging device and the base. The adhesive II used in any base bonding part has a high curing strength. Under the pre-positioning effect of the adhesive I, the fluorescence microscopy imaging device and the base can take into account both assembly accuracy and assembly strength.

[0062] In summary, the multi-light-path optical-mechanical imaging equipment provided by the present invention adopts an adhesive portion to ensure the assembly accuracy of a single fluorescence microscopy imaging device and greatly shortens the size of the assembly, and further combines the base adhesive portion to integrate multiple fluorescence microscopy imaging devices into the base with a high-precision and high-strength assembly effect. Therefore, the multi-light-path optical-mechanical imaging equipment can utilize all imaging optical paths of all fluorescence microscopy imaging devices to simultaneously observe multiple parts of the observation object, greatly improving the observation efficiency and reducing the operation frequency and equipment maintenance frequency.

[0063] The multi-light-path optical-mechanical imaging device provided by the present invention is further described below in conjunction with the accompanying drawings and implementation examples.

[0064] In the above embodiment, the bonding groove I can be set as a through groove that runs through from top to bottom, and the bonding groove II can be set as a recessed groove. The through groove and the bonding claw I inserted into the through groove are fixed by an adhesive I. This process requires that the adhesive I is quickly cured after the fluorescence microscopy imaging device and the base are accurately positioned, so the adhesive I can specifically use a UV-curing optical adhesive.

[0065] In order to confine the UV-curing optical adhesive in the through groove, transparent tape 03 can be used to temporarily block the outlet of the through groove in the open direction before the adhesive claw I is embedded in the through groove. For example, if the through groove is through from top to bottom, transparent tape 03 can be temporarily pasted on the open bottom of the through groove.

[0066] Compared with the sinking groove, the through groove has a larger exposed area. With the assistance of the transparent tape 03, it can not only meet the bonding and fixing of the UV-curing optical adhesive to the bonding claw I and the bonding groove I, but also facilitate more external ultraviolet light to irradiate the bonding claw I and the bonding groove I, thereby shortening the curing time of the UV-curing optical adhesive.

[0067] In particular, for the through groove that runs through the top and bottom and is arranged on the base, the UV-curing optical glue will expand downward and squeeze the transparent tape 03 when it is cured and expanded. Therefore, the fluorescence microscopy imaging device and its adhesive claw I are conducive to limiting the upward drift of the fluorescence microscopy imaging device relative to the base under the action of their own weight, thereby better ensuring the assembly accuracy of the fluorescence microscopy imaging device and the base.

[0068] Furthermore, the above-mentioned adhesive claw II is inserted into the sink from top to bottom. In other words, the entrance of the sink faces upward, and the adhesive claw II is inserted downward from the entrance of the sink into the sink. The above-mentioned adhesive claw II tapers from the middle of its height direction to the top, so the top of the adhesive claw II can be roughly prism-shaped, pyramid-shaped or cone-shaped. At the same time, the top of the adhesive claw II is different in shape from the top of the adhesive claw I. On the one hand, the top shape of the adhesive claw II is conducive to the operator to fill the adhesive II into the gap between the mutually nested adhesive claws II and the sink. On the other hand, the top shape of the adhesive claw II can be obviously distinguished from the shape of the adhesive claw I, which is convenient for the operator to accurately distinguish the adhesive claw I and the adhesive claw II, and then accurately add the adhesive I and the adhesive II into the adhesive claw I and the adhesive claw II. Of course, with respect to the top shape of the bonding claw II described above, the top of the bonding claw I can be set to a square shape, and the top of the square bonding claw I can be chamfered to facilitate the operator to fill the glue and ensure the operator's operating safety.

[0069] The following description will be made by taking three base bonding parts provided between any fluorescence microscopy imaging device and the base as an example.

[0070] Three base bonding parts are provided between any fluorescence microscopy imaging device and the base, and the three are respectively distributed according to the three vertices of an isosceles triangle. A base bonding part includes a bonding claw I, a bonding claw II, a bonding groove I and a bonding groove II, and the three base bonding parts include three bonding claws I, three bonding claws II, three bonding grooves I and three bonding grooves II. The aforementioned three bonding claws I and three bonding claws II are all provided in the fluorescence microscopy imaging device, and the three bonding claws I specifically include bonding claws I-I3201, bonding claws I-II3203 and bonding claws I-III3205, and the three bonding claws II specifically include bonding claws II-I3202, bonding claws II-II3204 and bonding claws II-III3206. The three bonding grooves I and the three bonding grooves II are all arranged on the base. The three bonding grooves I specifically include bonding groove II, bonding groove I-II and bonding groove I-III. The three bonding grooves II specifically include bonding groove II-I, bonding groove II-II and bonding groove II-III.

[0071] In the above example, the bonding groove II and the bonding claw I-I 3201 are nested, the bonding groove I-II and the bonding claw I-II 3203 are nested, and the bonding groove I-III and the bonding claw I-III 3205 are nested, and any pair of the aforementioned mutually nested structures are fixed by adhesive I. The above bonding groove II-I and the bonding claw II-I 3202 are nested, the bonding groove II-II and the bonding claw II-II 3204 are nested, and the bonding groove II-III and the bonding claw II-III 3206 are nested, and any pair of the aforementioned mutually nested structures are fixed by adhesive II.

[0072] In order to facilitate the operation of the operator, the three pairs of mutually nested structures of bonding groove II and bonding claw I-I 3201, bonding groove I-II and bonding claw I-II 3203, bonding groove I-III and bonding claw I-III 3205 can be distributed outside the other three pairs of mutually nested structures, such as Fig.13 and Fig.14 As shown, it is convenient for the operator to quickly, conveniently and accurately nest the bonding groove II and the bonding claw I-I3201, the bonding groove I-II and the bonding claw I-II3203, the bonding groove I-III and the bonding claw I-III3205 after the base and the fluorescence microscopy imaging device are precisely positioned, and it is also beneficial to replenish the adhesive I between the aforementioned three pairs of structures.

[0073] On the basis of the above structure, in the multi-light path optical imaging device, the base includes a crossbeam 2; all the base bonding parts between the same fluorescence microscopic imaging device and the base are fixed to the fluorescence microscopic imaging device as a whole through the microscopic imaging part 321, and through the base part 322, for example Fig.12The first base portion 3221 and the second base portion 3222 are fixed to the crossbeam 2 as a whole.

[0074] In the multi-light path optical machine imaging device, all fluorescence microscopic imaging devices are fixed to the crossbeam 2 at an angle such that all imaging light paths are parallel and spaced. In addition, a support plate 1 can be provided below the crossbeam 2 for quick connection with other devices.

[0075] For example, the multi-light path optical machine imaging device includes four fluorescence microscopic imaging devices, and the imaging light path of any fluorescence microscopic imaging device is perpendicular to the horizontal surface of the horizontally extended crossbeam 2, and the imaging light paths of the four fluorescence microscopic imaging devices are distributed in a rectangular array with the center of the aforementioned crossbeam 2 as the midpoint. Of course, for observation objects with different morphological characteristics, all fluorescence microscopic imaging devices of the multi-light path optical machine imaging device can also be distributed at other angles, such as evenly spaced distribution along the same straight line.

[0076] Furthermore, in the multi-light path optical machine imaging device provided by the present invention, all bonding parts may include a first bonding part provided between the camera assembly and the lighting assembly 9 and a second bonding part provided between the focusing assembly and the lighting assembly 9. At the same time, the normal line of any mortise and tenon joint surface 20 in the first bonding part is perpendicular or parallel to the first joint direction of the camera assembly and the lighting assembly 9, and the normal line of any mortise and tenon joint surface 20 in the second bonding part is perpendicular or parallel to the second joint direction of the focusing assembly and the lighting assembly 9.

[0077] The first adhesive portion and the second adhesive portion may be configured to have the same structural form or different structural forms. The following is a detailed description taking the first adhesive portion and the second adhesive portion having different structural forms as an example.

[0078] For the camera assembly and the lighting assembly 9 that need to be fixed by gluing, the normal line of any mortise and tenon joint surface 20 in the first bonding portion is perpendicular to the first joint direction of the camera assembly and the lighting assembly 9. Figures 5 to 7 The aforementioned splicing direction refers to Figure 5 and Figure 6 The horizontal direction and Figure 7 Therefore, for any mortise and tenon joint surface 20, it is parallel to the first joint direction of the camera assembly and the lighting assembly 9, and is also perpendicular to Figure 7 The paper where it is located.

[0079] Among them, all the mortise and tenon joint surfaces 20 may include a flat adhesive surface 201 and an oblique adhesive surface 202; the flat adhesive surface 201201 refers to the Figure 7 The horizontal or vertical surface of the paper surface, the oblique adhesive surface 202202 refers to the Figure 7 The inclined surface within the paper.

[0080] For the convenience of expression, Figure 7 The horizontal, vertical and Figure 7 The XZY axis coordinate system is established in the inside and outside directions of the paper. The Z axis corresponds to Figure 7 The inside-outside direction of the paper, which is also called the first splicing direction in this example; the X-axis and the Y-axis correspond to Figure 7 The horizontal direction and vertical direction of the adhesive surface 201 are also referred to as the first reference direction and the second reference direction in this example. Obviously, any two of the first splicing direction, the first reference direction and the second reference direction are perpendicular to each other. Therefore, the flat adhesive surface 201 refers to a surface parallel to the first reference direction or the second reference direction, and the oblique adhesive surface 202 refers to a surface that is neither parallel to the first reference direction nor parallel to the second reference direction.

[0081] by Figure 7 For example, all the shaded structures correspond to the camera assembly and all the blank frames correspond to the lighting assembly 9. The camera assembly and the lighting assembly 9 each include 8 mortise and tenon joint surfaces 20. These 16 mortise and tenon joint surfaces 20 are fitted in pairs to form 8 pairs of mutually glued mortise and tenon joint surfaces 20. Among the aforementioned 8 pairs of mutually glued mortise and tenon joint surfaces 20, any pair of mortise and tenon joint surfaces 20 is perpendicular to Figure 7 On the paper surface, two pairs of mortise and tenon joint surfaces 20 are horizontally distributed and parallel to each other, two pairs of mortise and tenon joint surfaces 20 are vertically distributed and parallel to each other, and the other four pairs of mortise and tenon joint surfaces 20 are obliquely distributed and parallel to each other.

[0082] The displacement changes of the 8 pairs of mortise and tenon joints 20 between the camera assembly and the lighting assembly 9 due to the expansion and contraction of the adhesive layer can be decomposed into the X-axis and the Y-axis. Since the adhesive layer between any pair of mortise and tenon joints 20 may expand or contract, and the expansion and contraction amounts are different, the stresses in different directions generated by the 8 pairs of mortise and tenon joints 20 can offset and compensate each other. Compared with using a single adhesive layer in a single direction to achieve the adhesive fixation of the camera assembly and the lighting assembly 9, the solution provided in the present application is conducive to improving the assembly accuracy of the camera assembly and the lighting assembly 9 in the X-axis and Y-axis directions and the relative position accuracy during use by offsetting and compensating for the expansion and contraction changes of all adhesive layers.

[0083] For the focusing assembly and the lighting assembly 9 that need to be fixed by gluing, the normal line of any mortise and tenon joint surface 20 in the second bonding portion is parallel to the second joint direction of the focusing assembly and the lighting assembly 9. Figure 8 and Fig. 9 The aforementioned second splicing direction corresponds to Figure 8 The diagonal line from the upper left corner to the lower right corner also corresponds to Fig. 9 Therefore, for any mortise and tenon joint surface 20, its vertical direction Fig. 9 Horizontal distribution.

[0084] Similar to the first bonding part, for all the mortise and tenon joint surfaces 20 in the second bonding part, multiple adhesive layers between all the mortise and tenon joint surfaces 20 can be used to offset and compensate for stress and expansion and contraction changes, thereby avoiding the focusing component and the lighting component 9 from being Fig. 9 The vertical direction produces position drift.

[0085] Furthermore, in the second bonding portion, all the mortise and tenon joint surfaces 20 can be distributed in multiple layers along the joint direction. Fig. 9 As shown, the focusing assembly and the lighting assembly 9 form a total of 10 mortise and tenon joint surfaces 20 at three glue application points, and these 20 mortise and tenon joint surfaces 20 are fitted in pairs to form 10 pairs of mortise and tenon joint surfaces 20.

[0086] At the same time, the projections of the above 10 pairs of mortise and tenon joint surfaces 20 are Fig. 9 The horizontal planes overlap. Fig. 9 The horizontal plane of can be regarded as the reference plane of the second bonding part, and this reference plane is perpendicular to the splicing direction between the focusing assembly and the lighting assembly 9. This setting is for the focusing assembly and the lighting assembly 9 that have a tendency to move relative to each other along the aforementioned splicing direction. Fig. 9 Therefore, under the premise that the focusing component and the lighting component 9 are glued and fixed to each other, the above-mentioned 10 pairs of mortise and tenon joint surfaces 20 utilize the overlapping projections of the 10 pairs of mortise and tenon joint surfaces 20 on the reference plane to further reduce the risk of the focusing component and the lighting component 9 detaching from each other at the adhesive.

[0087] The relative position setting of the above-mentioned multi-layer mortise and tenon joint surface 20 is equivalent to forming a safety redundancy at the bonding point of the focusing component and the lighting component 9, which can avoid the risks that are difficult to control during the bonding process, such as the amount of glue, the temperature and humidity of curing, the cavitation formed during the glue coating and other factors that cause adverse interference to the bonding effect.

[0088] Of course, the above example is only described by setting 10 pairs of mortise and tenon joints 20 between the focusing assembly and the lighting assembly 9. As for the number of mortise and tenon joints 20 between the focusing assembly and the lighting assembly 9 and other relative relationships except the angle, they can be specifically designed according to actual needs.

[0089] For example, see Fig. 9 In this application, the end of the voice coil motor 12 in the focusing assembly and the end of the lighting assembly 9 are both provided with a tenon head and a mortise portion in the shape of a chain tooth, so as to realize the mutual insertion connection between the focusing assembly and the lighting assembly 9, thereby forming a nested and glued second bonding portion.

[0090] In order to achieve better technical effects, the lighting assembly 9 used in the multi-light path optical imaging device provided by the present invention may include a lighting component, a lens sleeve 95, a main support 98, and multiple shaft kits 99 arranged between the lens sleeve 95 and the main support 98.

[0091] In the above-mentioned lighting assembly 9, a lens 94 is arranged in a lens sleeve 95, and a lighting component is installed above the lens. The main support 98 is provided with a mounting cavity and a plurality of adjustment cavities, the mounting cavity is used to accommodate and support the lens sleeve 95 upward, all the adjustment cavities correspond to all the shaft sleeves 99 of the lighting assembly 9 one by one, and any one of the adjustment cavities is used to constrain the degree of freedom of the shaft sleeve 99, so that the shaft sleeve 99 can only move and position along its own axial direction.

[0092] The shaft kit 99 refers to shaft sleeve parts, including but not limited to shaft-shaped, rod-shaped parts or sleeve-shaped parts.

[0093] In this position adjustment structure, two pairs of mounting surfaces 914 are provided on the circumferential side of the lens sleeve 95, and any one of the mounting surfaces 914 abuts against the shaft sleeve 99, and at least one mounting surface 914 abuts against two or more shaft sleeves 99. For the same pair of mounting surfaces 914, all the shaft sleeves 99 abutting therewith move in the same direction, that is, the axial directions of all the shaft sleeves 99 abutting therewith are in the same direction. For the two pairs of mounting surfaces 914, the moving direction of the shaft sleeve 99 abutting against the first pair of mounting surfaces 914 is perpendicular to the moving direction of the shaft sleeve 99 abutting against the second pair of mounting surfaces 914, and the above two moving directions are perpendicular to the vertical direction. In short, if we look at Fig.10 The vertical direction is the Z axis, and the shaft assembly 99 abutting against the first pair of mounting surfaces 914 and the shaft assembly 99 abutting against the second pair of mounting surfaces 914 move along the X axis and the Y axis respectively.

[0094] Since the shaft kit 99 is in contact with the mounting surface 914 of the lens sleeve 95 and cooperates with the axial hole of the adjustment cavity of the main support 98, when the shaft kit 99 moves axially along the adjustment cavity in which it is located, it can apply a force to the mounting surface 914 of the lens sleeve 95, so that the lens sleeve 95 moves or deflects in the mounting cavity of the main support 98, thereby adjusting the relative position of the lens sleeve 95 with the main support 98 as a reference.

[0095] In this embodiment, based on the moving direction of the shaft sleeve 99 in the adjustment cavity, the movement of the entire shaft sleeve 99 abutting against the lens sleeve 95 can be divided into movement along the X axis and movement along the Y axis. Fig.10 and Fig.11 ,by Fig.11 The horizontal direction is Fig.10 The X-axis direction. Fig.10 and Fig.11In the example provided, there are three shaft assemblies 99 that move along the X-axis, two of which are located on the right side of the lens tube 95 and the other one is located on the left side of the lens tube 95 .

[0096] When the three shaft sets 99 are all moved to the left, the lens sleeve 95 moves to the left of the X-axis relative to the main support 98; conversely, when the three shaft sets 99 are all moved to the right, the lens sleeve 95 moves to the right of the X-axis relative to the main support 98. The above process realizes the translation of the lens sleeve 95 relative to the main support 98 on the X-axis.

[0097] Among the three shaft sets 99, if only one of the two shaft sets 99 on the same side moves to the left, and the shaft set 99 on the opposite side moves to the left, the lens sleeve 95 rotates relative to the main support 98 around the Z axis, and the direction of rotation can be defined as the first direction of rotation; conversely, if only one of the two shaft sets 99 on the same side moves to the right, and the shaft set 99 on the opposite side moves to the right, the lens sleeve 95 rotates relative to the main support 98 around the Z axis, and the direction of rotation is the second direction of rotation. The first direction of rotation and the second option are opposite to each other.

[0098] Similarly, the multiple axis sets 99 moving along the Y axis can enable the lens sleeve 95 to achieve Y-axis translation and rotation around the Z axis relative to the main support 98.

[0099] In the above embodiment, for a pair of mounting surfaces 914 of the lens sleeve 95, two shaft sets 99 can satisfy the translation of the lens sleeve 95, and three shaft sets 99 can satisfy the rotation of the lens sleeve 95 around the Z axis. Therefore, for the lens sleeve 95, only one of the mounting surfaces 914 is required to abut against two or more shaft sets 99. It can be seen that in the multi-light path optical imaging device, the illumination assembly 9 uses the shaft set 99 located between the lens sleeve 95 and the main support 98 to achieve the relative position adjustment of the lens sleeve 95 and the main support 98. The structure is simple and the operation is convenient and fast, which is conducive to adjusting the fluorescence signal intensity in the observation area of ​​the lens 94, ensuring the uniform illumination intensity in the observation area, and thus improving the signal-to-noise ratio.

[0100] Furthermore, at least one pair of mounting surfaces 914 in the lens sleeve 95 may be configured as inclined surfaces. Here, an inclined surface is defined as a surface whose normal line is inclined upward.

[0101] For reference Fig.11 The outer periphery of the lens sleeve 95 is provided with a square positioning flange; the outer periphery of the square positioning flange includes four mounting surfaces 914 connected end to end, and the aforementioned four mounting surfaces 914 are opposite to each other and are used for the shaft sleeve 99 in the position adjustment structure to abut. For the mounting surface 914 on the right side of the lens sleeve 95, its normal line is Fig.11 For the mounting surface 914 on the left side of the lens sleeve 95, its normal line is Figure 2 The upper left corner of is tilted. Therefore, Fig.11 Under the premise that the multiple shaft assemblies 99 shown move along the X-axis, the force applied to the mounting surface 914 by any of the aforementioned shaft assemblies 99 can be decomposed into a component force for pressing the lens sleeve 95 downward. This component force is beneficial to cooperate with the supporting effect of the main support 98 on the lens sleeve 95 to improve the positioning effect of the lens sleeve 95 on the Z-axis and enhance the relative stability of the lens sleeve 95 and the main support 98.

[0102] In order to achieve better technical effects, the entire shaft assembly 99 of the lighting assembly 9 includes two spring plungers and a plurality of rigid ball plungers with external threads on the circumferential side. On this basis, for the two pairs of mounting surfaces 914 of the lens sleeve 95, one of any pair of mounting surfaces 914 abuts against a spring plunger and the other abuts against a rigid ball plunger. The adjustment cavity that cooperates with the shaft hole of the rigid ball plunger is specifically a threaded hole.

[0103] like Fig.10 As shown, the two pairs of mounting surfaces 914 of the lens sleeve 95 specifically include a first mounting surface 9141, a second mounting surface 9142, a third mounting surface 9143 and a fourth mounting surface, all of which are planar. The first mounting surface 9141 and the second mounting surface 9142 are spaced apart and face each other, the third mounting surface 9143 and the fourth mounting surface are spaced apart and face each other, the first mounting surface 9141 and the second mounting surface 9142 are respectively abutted against a spring plunger, the second mounting surface 9142 is abutted against two rigid ball plungers, and the third mounting surface 9143 is abutted against a rigid ball plunger.

[0104] More specifically, the spring plunger of the lighting assembly 9 may include a first spring plunger 9121 and a second spring plunger 9122 respectively abutting against one of the two pairs of mounting surfaces. Fig.10 and Fig.11 As shown, the first spring plunger 9121 abuts against the first mounting surface 9141, and the second spring plunger 9122 abuts against the fourth mounting surface. The rigid ball plunger of the lighting assembly 9 may specifically include a first ball plunger 9123, a second ball plunger 9124 and a third ball plunger 9125, which abut against the other of the two pairs of mounting surfaces, respectively. Fig.10 and Fig.11 As shown, the first ball plunger 9123 and the second ball plunger 9124 are both in contact with the second mounting surface 9142 , and the third ball plunger 9125 is in contact with the third mounting surface 9143 .

[0105] Since the rigid ball plunger is matched with the adjusting cavity thread, the operator can not only realize the axial movement of the rigid ball plunger by rotating the rigid ball plunger, but also accurately determine the axial displacement of the rigid ball plunger, thereby determining the displacement of the lens sleeve 95 along the X-axis and Y-axis and the rotation angle around the Z-axis. Of course, the positioning connection strength between the rigid ball plunger and the adjusting cavity can also be improved through the thread engagement. In short, when the operator does not operate the rigid ball plunger, the position of the rigid ball plunger in the adjusting cavity is not easily affected by external forces, which can effectively ensure the connection stability of the lens sleeve 95 and the main support 98.

[0106] In the above example, both the spring plunger and the rigid ball plunger are in point contact with the mounting surface 914. Compared with the rigid ball plunger, the spring plunger is elastic along its axial direction. Therefore, for any spring plunger, when one axial end is fixed, the other axial end can still move with the help of elasticity. Based on the aforementioned characteristics of the spring plunger, when adjusting the multiple shaft kits 99 abutting the same pair of mounting surfaces 914, only the rigid ball plungers in the aforementioned multiple shaft kits 99 can be adjusted. At this time, the spring plunger adaptively undergoes elastic deformation to meet the displacement requirements of the lighting assembly 9. It can be seen that the spring plunger can significantly simplify the adjustment operation of the lighting assembly 9, increase the operation speed, and reduce the difficulty of operation.

[0107] In addition, in the multi-light path optical imaging device, the lighting assembly 9 further includes a rigid sleeve positioning member 913 for moving in the vertical direction; one axial end of the rigid sleeve positioning member 913 is fixed to the lens sleeve 95 .

[0108] The rigid sleeve positioning member 913 also belongs to the sleeve type parts, such as Fig.10 The first set screw and the second set screw are used to move in the vertical direction, i.e., the Z-axis direction, to achieve the pitch of the lens sleeve 95 relative to the main support 98. One axial end of the rigid sleeve positioning member 913 is relatively fixed to the lens sleeve 95, and the other end extends to the outside of the lens sleeve 95, so that the operator can adjust the position of the rigid sleeve positioning member 913 along the Z-axis direction.

[0109] The rigid sleeve positioning member 913 may be specifically configured as a set screw.

[0110] In order to meet the pitching motion of the lens sleeve 95 at various angles, a plurality of rigid sleeve positioning members 913 are provided on the peripheral side of the lens sleeve 95. When adjusting the pitching angle of the lens sleeve 95, the operator can move one or more of the rigid sleeve positioning members 913 separately.

[0111] In the above lighting device, the lighting element can be specifically configured as a plate-shaped LED 93. A heat sink 91 can be disposed above the LED 93, and the LED 93 can be fixedly connected to the heat sink 91 by screws. The heat sink 91 is connected to the main support 98 through a guide column 92 and an adjustment column 97.

[0112] The guide post 92 is inserted into the hole of the heat sink 91, one end of the guide post 92 is locked to the surface of the heat sink 91 by the first nut 910, and the other end of the guide post 92 is inserted into the hole of the main support 98. One end of the adjustment post 97 is inserted into the hole of the heat sink 91 and locked to the heat sink 91 by the second nut 911, and the other end of the adjustment post 97 is screwed into the threaded hole of the main support 98. Therefore, combined with the guide post 92, the operator can rotate the adjustment post 97 to achieve the LED 93 and the heat sink 91 to move away from and approach the lens sleeve 95 and the lens 94 along the Z axis. After the adjustment is completed, the guide post positioning member 915 is used to lock and fix the guide post 92 and the main support 98.

[0113] Furthermore, the lens sleeve 95 is coaxially distributed with the lens 94 installed therein and the diaphragm 96 located therebelow and is fixedly connected. For example, the lens 94, the diaphragm 96 and the lens sleeve 95 can be glued together to ensure that the lens 94 and the diaphragm 96 move synchronously and stably with the lens sleeve 95 during adjustment.

[0114] In summary, the lighting device used in the multi-light path optical imaging device satisfies the system's adjustment of the relative positions of LED 93, aperture 96, and collimating lens of lens 94, and the entire product has a compact structure and small volume, and is quick to adjust and stable and reliable after adjustment. The lighting device of the fluorescence microscopy imaging system can ensure the light efficiency in the observation area so as to obtain the appropriate fluorescence signal intensity; at the same time, it can ensure the uniformity of the lighting in the observation area, so that the fluorescent molecules or fluorescent groups in the observation area can obtain the same intensity of light, thereby improving the signal-to-noise ratio.

[0115] When assembling the multi-light path optical imaging device, firstly, the different optical and optoelectronic components are reasonably grouped and assembled, for example, the industrial camera 4, the camera bracket 5, the camera mounting parts and other components are divided into the camera assembly and assembled into one, the LED 93, the collimating lens, the aperture 96, the filter, the dichroic mirror, the converging lens, the heat sink 91, the lighting mounting parts, the focusing light source assembly 13, the tube lens 7 and the tube lens bracket 8 and other components are divided into the lighting assembly 9 and assembled into one, and the objective lens 11, the voice coil motor 12, the voice coil motor mounting parts, the objective lens seat 10 and other components are divided into the focusing assembly and assembled into one. Secondly, the multi-degree-of-freedom adjustment device and the external detection device are used to assist in positioning the camera assembly and the tube lens 7 of the lighting assembly 9, so as to achieve the gluing fixation of the industrial camera 4 and the tube lens 7, and the multi-degree-of-freedom adjustment device and the external detection device are used to assist in positioning the lighting assembly 9 and the voice coil motor 12 of the focusing assembly, so as to achieve the gluing fixation of the lighting assembly 9 and the voice coil motor 12, thereby completing the assembly operation of a single fluorescence microscopy imaging device. Furthermore, the multi-degree-of-freedom adjustment device and the external detection device are used to assist in positioning the base and any fluorescence microscopic imaging device, so as to achieve gluing and fixing of all fluorescence microscopic imaging devices on the base. A dustproof ring 6 is provided between the industrial camera 4 and the tube lens 7 .

[0116] In the above operation, the multi-degree-of-freedom adjustment device uses the multi-degree-of-freedom adjustment device connector 01 to position and connect the components of the multi-path optical imaging device, and the external detection device uses the detection device connector 02 to position and connect the components of the multi-path optical imaging device.

[0117] The above is a detailed introduction to the multi-light path optical imaging device provided by the present invention. This article uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principles of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A multi-light path optical machine imaging device, characterized in that: It comprises a base and a plurality of fluorescence microscopic imaging devices positioned and installed on the base; Any of the fluorescence microscopic imaging devices has an imaging optical path, and all of the imaging optical paths of all of the fluorescence microscopic imaging devices are arranged at intervals; any of the fluorescence microscopic imaging devices comprises a camera assembly having an industrial camera (4), an illumination assembly (9) connected to a tube lens (7), and a focusing assembly having a voice coil motor (12); the camera assembly, the illumination assembly (9), and the focusing assembly in the same fluorescence microscopic imaging device are interconnected, and a bonding portion is provided between two adjacent ones; any of the bonding portions comprises a plurality of mortise and tenon joint surfaces (20) that are mutually matched, nested, and fixed by gluing; any pair of mutually gluing mortise and tenon joint surfaces (20) in the same bonding portion are arranged opposite to and not perpendicular to at least one pair of another mutually gluing mortise and tenon joint surfaces (20); There are three or more non-collinear base bonding parts between any of the fluorescence microscopy imaging devices and the base; any of the base bonding parts includes bonding claw I, bonding groove I, bonding claw II and bonding groove II; The bonding claw I and the bonding groove I are respectively arranged on the base and the fluorescence microscopy imaging device and are bonded and fixed by an adhesive I; The bonding claw II and the bonding groove II are respectively arranged on the base and the fluorescence microscopy imaging device and are fixed by adhesive II; the curing rate of the adhesive I is greater than the curing rate of the adhesive II, and the bonding strength of the adhesive II is greater than the bonding strength of the adhesive I.

2. The multi-light path optical machine imaging device according to claim 1, characterized in that: The bonding groove I is a through groove that runs through from top to bottom; the bonding groove II is a sink groove; and the adhesive I is specifically a UV-curing optical adhesive.

3. The multi-light path optical machine imaging device according to claim 2, characterized in that: The bonding claw II is inserted into the sink from top to bottom, and the bonding claw II gradually shrinks from the middle to the top in the height direction; the top of the bonding claw I is different in shape from the top of the bonding claw II.

4. The multi-light path optical mechanical imaging device according to any one of claims 1 to 3, characterized in that: All of the bonding parts include a first bonding part provided between the camera assembly and the lighting assembly (9) and a second bonding part provided between the focusing assembly and the lighting assembly (9); A normal line of any of the mortise and tenon joint surfaces (20) in the first bonding portion is perpendicular or parallel to a first joint direction of the camera assembly and the lighting assembly (9); The normal line of any of the mortise and tenon joint surfaces (20) in the second bonding portion is perpendicular or parallel to the second joint direction of the focusing assembly and the lighting assembly (9).

5. The multi-light path optical machine imaging device according to claim 4, characterized in that: The normal of any of the mortise and tenon joint surfaces (20) in the first bonding portion is perpendicular to the first joint direction; all of the mortise and tenon joint surfaces (20) in the first bonding portion include a flat adhesive surface (201) and an oblique adhesive surface (202); the flat adhesive surface (201) is parallel to a first reference direction or a second reference direction; the oblique adhesive surface (202) is not parallel to the first reference direction, and the oblique adhesive surface (202) is not parallel to the second reference direction; wherein the first reference direction, the second reference direction and the first joint direction are perpendicular to each other.

6. The multi-light path optical machine imaging device according to claim 4, characterized in that: The normal of any of the mortise and tenon joint surfaces (20) in the second bonding portion is parallel to the second joint direction; all of the mortise and tenon joint surfaces (20) in the second bonding portion are spaced apart in multiple layers along the second joint direction, and the projections of all of the mortise and tenon joint surfaces (20) overlap in a reference plane; and the reference plane is perpendicular to the second joint direction.

7. The multi-light path optical machine imaging device according to claim 5, characterized in that: The ends of the voice coil motor (12) and the ends of the lighting assembly (9) are both provided with a tenon head and a mortise eye in the shape of chain teeth.

8. The multi-light path optical mechanical imaging device according to any one of claims 1 to 3, characterized in that: The lighting assembly (9) comprises a lighting element, a lens sleeve (95), a main support (98), and a plurality of shaft sleeves (99) arranged between the lens sleeve (95) and the main support (98); The lighting component is arranged above the lens (94) in the lens sleeve (95); The main support (98) is provided with a mounting cavity for accommodating and supporting the lens sleeve (95) upwards, and a plurality of adjustment cavities for all the shaft sleeves (99) to achieve axial movement and positioning in a one-to-one correspondence; two pairs of mounting surfaces (914) are provided on the circumferential side of the lens sleeve (95); any of the mounting surfaces (914) abuts against the shaft sleeve (99); at least one of the mounting surfaces (914) abuts against two or more shaft sleeves (99); all the shaft sleeves (99) abutting against the same pair of mounting surfaces (914) move in the same direction, and the movement directions of the shaft sleeves (99) abutting against the two pairs of mounting surfaces (914) are perpendicular to each other and perpendicular to the vertical direction.

9. The multi-light path optical machine imaging device according to claim 8, characterized in that: The entire shaft kit (99) includes two spring plungers and multiple rigid ball plungers with external threads on the circumferential side; one of any pair of mounting surfaces (914) abuts against one of the spring plungers and the other abuts against the rigid ball plunger; the adjustment cavity that cooperates with the rigid ball plunger shaft hole is specifically a threaded hole.

10. The multi-light path optical machine imaging device according to claim 8, characterized in that: It also includes a rigid sleeve positioning piece (913) for moving in the vertical direction; one axial end of the rigid sleeve positioning piece (913) is relatively fixed to the lens sleeve (95).

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