Alpha-amylase variants
By modifying the parental α-amylase at specific positions, an α-amylase variant with improved sequence identity is formed, which solves the problem of insufficient stability and efficiency of existing α-amylases at low temperatures and improves the stain removal effect of washing clothes and tableware.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NOVOZYMES AS
- Filing Date
- 2020-12-18
- Publication Date
- 2026-06-19
AI Technical Summary
Existing α-amylases lack stability and efficiency in detergents, especially in low-temperature and short-time washing cycles, making it difficult to effectively remove starch-containing stains.
By introducing modifications at specific sites in the parental α-amylase, α-amylase variants are formed, improving their sequence identity and washing performance, including maintaining or enhancing enzyme activity and stability at low temperatures.
It achieves improved washing performance of α-amylase at low temperatures, enhancing its stain removal ability in washing clothes and tableware, and is suitable for household cleaning.
Smart Images
Figure QLYQS_1 
Figure QLYQS_2 
Figure QLYQS_3
Abstract
Claims
1. An α-amylase variant of the parental α-amylase of SEQ ID NO: 1, wherein any one of the following modification groups is introduced into the parental α-amylase of SEQ ID NO: 1: The variant is numbered using SEQ ID NO: 1, and the variant described therein has α-amylase activity.
2. A method of improving the detergency performance of an alpha-amylase variant of a parent alpha-amylase, said method comprising the steps of: Introduce any of the modifications of claim 1 into the parental α-amylase of SEQ ID NO: 1, wherein the variant has α-amylase activity and improved washing performance relative to the parental polypeptide.
3. Use of the α-amylase variant according to claim 1 in a cleaning process.
4. The use according to claim 3, wherein the cleaning process is laundry or hard surface cleaning including dishwashing and industrial cleaning.
5. A composition comprising the variant according to claim 1.
6. The composition according to claim 5, further comprising at least one additional active component.
7. The composition of claim 6, wherein the active component is selected from the group consisting of: surfactants, flocculants, chelating agents, dye transfer inhibitors, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleaching activators, hydrogen peroxide, hydrogen peroxide sources, pre-formed peracids, clay detergents / anti-redeposition agents, defoamers, dyes, fragrances, structural elasticizers, fabric softeners, carriers, water-soluble agents, fabric colorants, antifoaming agents, processing aids, and / or pigments.
8. The composition according to claim 6, wherein the active component is a dispersant, a detergent builder, a co-detergent builder, and / or a brightening agent.
9. The composition of claim 8, wherein the dispersant is a polymeric dispersant.
10. The composition of claim 7, wherein the active component is an enzyme.
11. The composition of claim 10, wherein the enzyme is selected from the group consisting of: proteases, amylases, lipases, keratins, cellulases, xyloglucanases, pectins, xanthan gums, peroxidases, halogenated peroxidases, catalases, and mannanases, or any mixture thereof.
12. The composition of claim 10, wherein the enzyme is selected from the group consisting of pectin lyase and endoglucanase.
13. The composition of claim 5, wherein the composition comprises a surfactant.
14. The composition of claim 13, wherein the composition comprises 0.1-60 wt% surfactant.
15. The composition of claim 14, wherein the composition comprises 0.5-50 wt% of a surfactant.
16. The composition of claim 15, wherein the composition comprises 1-40 wt% of a surfactant.
17. The composition according to any one of claims 13-16, wherein the composition comprises a surfactant system, the surfactant system comprising a mixture of more than one surfactant.
18. The composition of claim 5, wherein the composition comprises a surfactant selected from the group consisting of anionic surfactants, nonionic surfactants including semi-polar surfactants, cationic surfactants, facultative zwitterionic surfactants, amphoteric surfactants, and mixtures thereof.
19. The composition of claim 18, wherein the anionic surfactant is a sulfonate surfactant and a sulfate surfactant, and / or optionally an alkoxylated alkyl sulfate.
20. The composition of claim 19, wherein the anionic surfactant is an alkylbenzene sulfonate.
21. The composition according to claim 19, wherein the anionic surfactant is a linear alkylbenzene sulfonate (LAS).
22. The composition according to any one of claims 18-19, wherein the anionic surfactant is an alkyl sulfate, and the alkyl sulfate includes alkyl ether sulfate.
23. The composition of claim 18, wherein the anionic surfactant is a C9-15 alcohol ether sulfate, a C8-C16 ester sulfate, or a C10-C14 ester sulfate.
24. The composition of claim 23, wherein the anionic surfactant is one of the C9-15 alcohol ether sulfates having an average degree of ethoxylation of 0.5-7.
25. The composition of claim 24, wherein the anionic surfactant is one of the C9-15 alcohol ether sulfates having an average degree of ethoxylation of 1-5.
26. The composition of claim 18, wherein the anionic surfactant is monododecyl sulfate.
27. The composition of claim 13, wherein the composition comprises an anionic surfactant.
28. The composition of claim 27, wherein the composition comprises an alkylbenzene sulfonate and / or optionally an ethoxylated alkyl sulfate.
29. The composition of claim 28, wherein the optionally ethoxylated alkyl sulfate has a degree of ethoxylation of 0-7.
30. The composition of claim 28, wherein the optionally ethoxylated alkyl sulfate has a degree of ethoxylation of 0.5-3.
31. The composition of claim 27, wherein the anionic surfactant is an isomer of LAS, branched alkylbenzene sulfonate (BABS), phenyl alkyl sulfonate, olefin sulfonate, alkyl sulfate (AS), secondary alkyl sulfonate (SAS), paraffin sulfonate (PS), ester sulfonate, sulfonated fatty acid glycerides, α-sulfo fatty acid methyl esters, alkyl succinic acid or alkenyl succinic acid, dodecenyl / tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters or fatty acid salts of sulfosuccinic acid, or combinations thereof, wherein the α-sulfo fatty acid methyl ester includes methyl ester sulfonate.
32. The composition of claim 27, wherein the anionic surfactant is an α-olefin sulfonate (AOS), an olefin sulfonate, an alkyl-2,3-dimethyl disulfate, a hydroxyalkyl sulfonate, or a disulfonate.
33. The composition of claim 23, wherein the alcohol ether sulfate is an alcohol ethoxy sulfate or a fatty alcohol ether sulfate, or the alkyl sulfate is sodium dodecyl sulfate (SDS), fatty alcohol sulfate (FAS), or primary alcohol sulfate (PAS).
34. The composition of claim 18, wherein the anionic surfactant is added to the cleaning composition in the form of a salt.
35. The composition of claim 18, wherein the composition comprises a nonionic surfactant.
36. The composition of claim 18, wherein the composition comprises anionic surfactant and nonionic surfactant.
37. The composition of claim 36, wherein the composition comprises anionic surfactant and nonionic surfactant, the weight ratio of anionic surfactant to nonionic surfactant being from 30:1 to 1:
2.
38. The composition of claim 37, wherein the weight ratio is 20:1 to 2:3 or 1:
1.
39. The composition of claim 18, wherein the composition comprises a nonionic surfactant, the nonionic surfactant comprising alcohol ethoxylates, alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkyl polysaccharides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine, and products available under the trade names SPAN and TWEEN, or combinations thereof.
40. The composition of claim 39, wherein the N-acyl N-alkyl derivative of glucosamine is glucosamide or fatty acid glucosamide, and the alkoxylated fatty acid alkyl ester is an ethoxylated and / or propoxylated fatty acid alkyl ester.
41. The composition of claim 13, wherein the composition comprises 1% to 40% of anionic surfactant.
42. The composition of claim 13, wherein the composition comprises 0.2% to 40% of a nonionic surfactant.
43. The composition of claim 42, wherein the nonionic surfactant is an alcohol ethoxylate, a nonylphenol ethoxylate, an alkyl polysaccharide, an alkyl dimethylamine oxide, an ethoxylated fatty acid monoethanolamide, a fatty acid monoethanolamide, a polyhydroxyalkyl fatty acid amide, or an N-acyl N-alkyl derivative of glucosamine.
44. The composition of claim 13, wherein the composition is selected from the group consisting of liquid detergents, solid detergents, gel detergents, powder detergents, tablets, pods, sachets and granules.
45. The composition according to claim 13, wherein the composition is a liquid laundry detergent or liquid tableware detergent composition or a solid laundry detergent composition or a powdered tableware detergent composition.
46. The composition of claim 45, wherein the liquid laundry or liquid dishwashing composition is an automatic dishwashing (ADW) liquid detergent composition, and the solid laundry composition is a soap bar.
47. The composition of claim 45, wherein the powdered dishwashing composition is an ADW unit dose detergent composition.
48. A polynucleotide encoding the α-amylase variant according to claim 1.
49. A nucleic acid construct comprising the polynucleotide according to claim 48.
50. An expression vector comprising the polynucleotide according to claim 48.
51. A host cell comprising the polynucleotide of claim 48, the nucleic acid construct of claim 49, or the expression vector of claim 50.