A pharyngeal swab loaded with reticular MOF black phosphorus nanocomposite for SERS detection and a preparation method thereof

Abstract

The disclosure provides a throat swab loaded with a reticular MOF black phosphorus nanocomposite for SERS detection and a preparation method thereof, the throat swab is composed of a throat swab sampling cotton swab and a reticular MOF coated sheet-shaped black phosphorus, the reticular MOF coated sheet-shaped black phosphorus is coated on the head of the throat swab sampling cotton swab; the foam hole density of the head of the throat swab sampling cotton swab is 300 ppi; the reticular MOF has a pore size of 200-300 nanometers and a nanoscale gap structure on the surface; the sheet-shaped black scale has a diameter of 1000-5000 nanometers; the black phosphorus has good biocompatibility with the reticular MOF, the throat swab sampling cotton swab is used as a bearing matrix, and the throat swab is simple to make and low in cost, and the open reticular foam structure of the throat swab is helpful for complete release of a sample into a culture solution, and a large number of protruding structures on the surface of the throat swab can further enhance the Raman signal of an adsorbed molecule.

Description

Technical Field

[0001] This disclosure relates to the fields of materials engineering and nanotechnology, specifically to a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection and its preparation method. Background Technology

[0002] The statements in this section are merely background information relating to this disclosure and do not necessarily constitute prior art.

[0003] In recent years, surface-enhanced Raman scattering (SERS) has become an advanced, non-invasive analytical technique capable of single-molecule detection. The significant amplification of localized electromagnetic fields formed on the surfaces of traditional noble metal nanostructures under external excitation light gives SERS high detection sensitivity. However, the preparation of noble metal nanoparticles typically requires biotoxic surfactants. Furthermore, under external excitation light, the localized temperature rise in electromagnetic hotspot regions can lead to deformation of the metal nanostructure, causing desorption or decomposition of target molecules, limiting the widespread application of SERS immunoassay in clinical biomedical testing. Therefore, non-metallic SERS immunoassays will open new avenues for early cancer immunodetection and treatment based on novel spectroscopic technologies. Among these, black phosphorus and network MOF composite materials have demonstrated good SERS sensitivity in the detection of COVID-19 antigens and can be used for direct detection of the COVID-19 antigen.

[0004] However, there is currently no good solution for applying black phosphorus and reticulated MOF composite materials to the preparation of pharyngeal swabs, nor is there a good solution for effectively combining this substrate with the preparation of pharyngeal swabs. Most current solutions have relatively low Raman signals for adsorbed molecules and cannot effectively enrich and immobilize molecules. Summary of the Invention

[0005] To address the aforementioned issues, this disclosure proposes a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection and its preparation method. The black phosphorus and network MOF composite material exhibit good biocompatibility, and the network structure has the function of enriching and immobilizing molecules, which will greatly enhance the Raman signal of adsorbed molecules.

[0006] According to some embodiments, the present disclosure adopts the following technical solutions:

[0007] A throat swab loaded with a mesh MOF black phosphorus nanocomposite material for SERS detection, the throat swab consisting of a throat swab sampling swab and a sheet of black phosphorus coated with mesh MOF; the sheet of black phosphorus coated with mesh MOF is coated on the head of the throat swab sampling swab.

[0008] Furthermore, the foam pore density of the pharyngeal swab sampling swab head is 300 ppi.

[0009] Furthermore, the mesh MOF has a pore size of 200-300 nanometers and a nanometer-level gap structure on its surface.

[0010] Furthermore, the diameter of the flaky black phosphorus is 1000-5000 nanometers.

[0011] According to other embodiments, the present disclosure adopts the following technical solutions:

[0012] A method for preparing a throat swab loaded with a mesh MOF black phosphorus nanocomposite for SERS detection includes:

[0013] Step (1): Take a small amount of lumpy black phosphorus and grind it into powder in a glove box. Then weigh the black phosphorus powder and polyvinylpyrrolidone and add them to anhydrous ethanol solution. Place them in an ultrasonic bath and ultrasonically peel them to obtain a brown dispersion. Centrifuge to remove the unpeeled lumpy black phosphorus. Centrifuge the upper dispersion to obtain polyvinylpyrrolidone modified black phosphorus flakes. Wash with methanol and collect the precipitate. Then redisperse it in methanol solution to obtain black phosphorus flake dispersion and store it in a refrigerator.

[0014] Step (2): Take a small amount of the black phosphorus tablet dispersion prepared in step (1) and drop it onto the pharyngeal swab to moisten the foam pores on the surface of the swab. Let it stand at room temperature. After the swab dries, add cobalt nitrate hexahydrate methanol solution dropwise and let it stand at room temperature. After it dries, add 2-methylimidazole methanol solution dropwise and then wash it. Place it in a petri dish and let it dry at room temperature to obtain a functionalized pharyngeal swab of mesh MOF black phosphorus nanocomposite material.

[0015] Furthermore, the method involves taking 50mg-100mg of blocky black phosphorus and 30ml of anhydrous ethanol solution.

[0016] Furthermore, 6 ml of methanol solution; 150 μl of cobalt nitrate hexahydrate methanol solution; and 60 μl of 2-methylimidazole methanol solution.

[0017] Furthermore, the cobalt nitrate hexahydrate methanol solution was added dropwise in four separate additions.

[0018] Furthermore, the 2-methylimidazole methanol solution is added dropwise in two steps.

[0019] Compared with the prior art, the beneficial effects of this disclosure are as follows:

[0020] This disclosure provides a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection. First, black phosphorus and the network MOF exhibit good biocompatibility. Phosphoric acid, a decomposition product of black phosphorus, is an electrolyte involved in metabolism in the human body, and imidazole, the main raw material of MOF, is an important component of histidine in the human body, providing conditions for direct sampling with functionalized swabs. Second, the network MOF, resembling a "virus trap" structure, is used to capture and adsorb viral antigens with a size of approximately 100 nm, and plays a role in enriching and immobilizing molecules, greatly enhancing the Raman signal of the adsorbed molecules. Third, black phosphorus, as a novel two-dimensional nanomaterial, has a higher Raman enhancement effect. In a liquid environment, it acts as a stable "nano island," used to immobilize nanoparticles and form numerous SERS "hot spots" in the gaps. Fourth, using a throat swab sampling swab as the carrier matrix is ​​simple to manufacture and low in cost. Its open network foam structure helps the sample to be completely released into the culture medium, and the surface also has numerous protrusions, which can further enhance the Raman signal of the adsorbed molecules. Attached Figure Description

[0021] The accompanying drawings, which form part of this disclosure, are used to provide a further understanding of this disclosure. The illustrative embodiments of this disclosure and their descriptions are used to explain this disclosure and do not constitute an undue limitation of this disclosure.

[0022] Figure 1 This is a scanning electron microscope image of the mesh MOF prepared in Example 1 of this disclosure;

[0023] Figure 2 This is a scanning electron microscope image of the black phosphorus sheet prepared in Example 1 of this disclosure;

[0024] Figure 3 This is a scanning electron microscope image of the functionalized throat swab sampling swab head of the mesh MOF black phosphorus nanocomposite material prepared in Example 1 of this disclosure;

[0025] Figure 4 The results of Raman detection of methylene blue on the three nanocomposite materials prepared in Example 3 of this disclosure: mesh MOF, black phosphorus sheet, and mesh MOF-black phosphorus sheet.

[0026] Figure 5 The results of Raman spectroscopy of the SARS-CoV-2 antigen on the functionalized pharyngeal swab prepared in Example 3 of this disclosure, which is loaded with a network of MOF black phosphorus nanocomposite material.

[0027] Figure 6 This is a scanning electron microscope image of a functionalized pharyngeal swab sampling swab made of the mesh MOF black phosphorus nanocomposite material prepared in Example 4 of this disclosure;

[0028] Figure 7The results of Raman spectroscopy of the SARS-CoV-2 antigen on the functionalized pharyngeal swab prepared in Example 4 of this disclosure, which is loaded with a network of MOF black phosphorus nanocomposite material.

[0029] Figure 8 The results of Raman spectroscopy of the SARS-CoV-2 antigen on the functionalized pharyngeal swab of the mesh MOF black phosphorus nanocomposite material prepared in Example 5 of this disclosure are shown.

[0030] Figure 9 The results of Raman spectroscopy of the SARS-CoV-2 antigen on the functionalized pharyngeal swab prepared in Example 5 of this disclosure, which is loaded with a network of MOF black phosphorus nanocomposite material. Detailed implementation method:

[0031] The present disclosure will be further described below with reference to the accompanying drawings and embodiments.

[0032] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this disclosure. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains.

[0033] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this disclosure. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms “comprising” and / or “including” are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0034] Example 1

[0035] This disclosure provides a throat swab loaded with a mesh MOF black phosphorus nanocomposite material for SERS detection in this embodiment. The throat swab consists of two parts: a throat swab sampling swab and a sheet of black phosphorus coated with mesh MOF. The sheet of black phosphorus coated with mesh MOF is coated on the head of the throat swab sampling swab.

[0036] The method used was a commercially available throat swab sampling swab. The swab's head had a foam pore density of 300 ppi and a MOF (Mesh-on-Foil) pore size of 200-300 nanometers, indicating a large number of nanoscale interstitial structures. The black phosphorus sheet had a diameter of 1000-5000 nanometers. Figure 1 As shown, MOF exhibits a network structure and its surface has depressions.

[0037] Depend on Figure 2 As shown, the size of the black phosphorus flakes is 1000-2000 nanometers.

[0038] Example 2

[0039] This disclosure provides a method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection in this embodiment, including the following steps:

[0040] Step (1): Take a small amount of blocky black scales and grind them into powder in a glove box. Then weigh the black scale powder and polyvinylpyrrolidone and add them to anhydrous ethanol solution. Place them in an ultrasonic bath and ultrasonically peel them to obtain a brown dispersion. Centrifuge to remove the unpeeled blocky black scales. Centrifuge the upper dispersion to obtain polyvinylpyrrolidone modified black phosphorus flakes. Wash with methanol and collect the precipitate. Then redisperse it in methanol solution to obtain black scale flake dispersion and store it in a refrigerator.

[0041] Step (2): Take a small amount of the black phosphorus dispersion prepared in step (1) and add it to the pharyngeal swab to moisten the foam pores on the surface of the swab. Let it stand at room temperature. After the swab dries, add cobalt nitrate hexahydrate methanol solution dropwise and let it stand at room temperature. After it dries, add 2-methylimidazole methanol solution dropwise. The purpose is to allow cobalt ions to combine with black phosphorus first and then form an organic framework structure in situ. Then wash, place in a petri dish, and let it stand at room temperature to dry, thus obtaining a functionalized pharyngeal swab of mesh MOF black phosphorus nanocomposite material.

[0042] In specific implementation, in step (1), a small amount of 50-100 mg of blocky black phosphorus is taken and ground into black phosphorus powder in a glove box. 30 mg of black phosphorus powder and 90-150 mg of polyvinylpyrrolidone are added together to a certain amount of anhydrous ethanol solution, which is 30 ml. The resulting solution is then placed in an ultrasonic bath for 4 hours to ultrasonically peel off the black phosphorus and obtain a brown dispersion. After ultrasonication, the unpeeled blocky black phosphorus is removed by centrifugation at 2000 pm for 15 min. The upper dispersion is centrifuged at 10000 pm for 10 min to obtain polyvinylpyrrolidone-modified black phosphorus flakes. After washing with methanol three times, the precipitate is removed, excess polyvinylpyrrolidone is washed away, and the sample is redispersed in 6 ml of methanol solution to prevent sample agglomeration. Then, it is packaged and stored in a refrigerator at 4°C.

[0043] In step (2), a small amount (100-200 μL) of the black phosphorus tablet dispersion prepared in step (1) is added dropwise to a pharyngeal swab until the foam pores on the surface of the swab are moistened, and left to stand at room temperature. After the swab dries, 6 mg / mL of cobalt nitrate hexahydrate methanol solution is added dropwise in several batches. The purpose of adding dropwise is to allow cobalt ions to combine with black phosphorus first, and then form an organic framework structure in situ. After standing at room temperature and drying, 6 mg / mL of 2-methylimidazole methanol solution is added dropwise in several batches. After drying, the swab is placed in 6 mg / mL of cobalt nitrate hexahydrate methanol solution for gentle washing, mainly to wash away unreacted 2-methylimidazole. The unreacted 2-methylimidazole on the surface of the swab is rinsed off. After the swab is moistened, it is immediately removed and placed in a petri dish, and left to dry at room temperature to obtain a functionalized pharyngeal swab of mesh MOF black phosphorus nanocomposite material.

[0044] The total volume of the cobalt nitrate hexahydrate methanol solution is 150 μl; the total volume of the 2-methylimidazolium methanol solution is 60 μl.

[0045] Example 3

[0046] This disclosure provides a method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection in this embodiment, including the following steps:

[0047] Step (1): Take a small amount of lumpy black phosphorus (50-100 mg) and grind it into black phosphorus powder in a glove box. Add the black phosphorus powder (30 mg) and polyvinylpyrrolidone (90 mg) to a certain amount of anhydrous ethanol solution (30 mL) and sonicate at 200 W for 4 hours under ice bath conditions to obtain a brown dispersion. Centrifuge at 2000 pm for 15 minutes to remove the lumpy black phosphorus that has not been completely broken up, and then centrifuge at 10000 pm for 10 minutes to obtain the precipitate. After washing with methanol three times and centrifuging, collect the precipitate and redisperse it in methanol solution (6 mL) to obtain the black phosphorus flake dispersion.

[0048] In step (2), a small amount of the black phosphorus tablet dispersion (100 μL) prepared in step (1) is added dropwise to a pharyngeal swab until it just wets the foam pores on the surface of the swab, and left to stand at room temperature. After the swab dries, cobalt nitrate methanol hexahydrate solution (100 μL in total, 6 mg / mL) is added dropwise in four separate additions, and left to stand at room temperature. After drying, 2-methylimidazole methanol solution (40 μL in total, 6 mg / mL) is added dropwise twice more. The dried swab is then gently washed in cobalt nitrate methanol hexahydrate solution (6 mg / mL) to rinse away any unreacted 2-methylimidazole on the surface of the swab. After wetting the swab, it is immediately removed and placed in a petri dish, and left to dry at room temperature to obtain the functionalized pharyngeal swab of the mesh MOF black phosphorus nanocomposite material.

[0049] Figure 3This is a scanning electron microscope image of a functionalized pharyngeal swab sampling swab containing the mesh-like MOF black phosphorus nanocomposite material prepared in Example 3 of this invention; from Figure 3 It can be seen that the network MOF grows uniformly on the black phosphorus sheet.

[0050] Figure 4 The results show the Raman spectral analysis of methylene blue by the three nanocomposite materials prepared in Example 3 of this invention: mesh MOF, black phosphorus sheet, and mesh MOF-black phosphorus nanocomposite. Figure 4 It can be seen that, compared with the latter two, the SERS enhancement effect of the mesh MOF black phosphorus nanocomposite on methylene blue is three times that of the former two, which proves the excellent SERS properties of the functionalized pharyngeal swab sampling swab loaded with mesh MOF black phosphorus nanocomposite prepared in this patent.

[0051] Figure 5 The image shows the Raman spectral detection results of the SARS-CoV-2 antigen on a functionalized pharyngeal swab prepared in Example 3 of this invention, which is a swab loaded with a network of MOF black phosphorus nanocomposite material. Figure 5 It can be seen that the functionalized sandpaper has a good SERS signal enhancement effect, with a Raman signal intensity of 1234 at 1066 cm⁻¹.

[0052] Example 4

[0053] This disclosure provides a method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection in this embodiment, including the following steps:

[0054] Step (1): Take a small amount of lumpy black phosphorus (50-100 mg) and grind it into black phosphorus powder in a glove box. Add the black phosphorus powder (30 mg) and polyvinylpyrrolidone (120 mg) to a certain amount of anhydrous ethanol solution (30 mL) and sonicate at 200 W for 4 hours under ice bath conditions to obtain a brown dispersion. Centrifuge at 2000 pm for 15 minutes to remove the incompletely super-digested lumpy black phosphorus, and then centrifuge at 10000 pm for 10 minutes to obtain the precipitate. After washing with methanol three times and centrifuging, collect the precipitate and redisperse it in methanol solution (6 mL) to obtain the black phosphorus flake dispersion.

[0055] Step (2): Take a small amount (150 μL) of the black phosphorus tablet dispersion prepared in step 1) and add it dropwise to a pharyngeal swab until it just wets the foam pores on the surface of the swab. Let it stand at room temperature. After the swab dries, add cobalt nitrate methanol hexahydrate solution (150 μL in total, 6 mg / mL) in four separate drops. Let it stand at room temperature and dry. Then add 2-methylimidazole methanol solution (60 μL in total, 6 mg / mL) in two more drops. Place the dried swab in cobalt nitrate methanol hexahydrate solution (6 mg / mL) and wash it gently to rinse off any unreacted 2-methylimidazole on the surface of the swab. After wetting the swab, immediately remove it and place it in a petri dish. Let it stand at room temperature to dry, and you will get the functionalized pharyngeal swab of the mesh MOF black phosphorus nanocomposite material.

[0056] Figure 6 This is a scanning electron microscope image of a functionalized pharyngeal swab sampling swab containing the mesh-like MOF black phosphorus nanocomposite material prepared in Example 4 of this invention; from Figure 6 It can be seen that the network MOF grows uniformly on the black phosphorus sheet.

[0057] Figure 7 The image shows the Raman spectral detection results of the SARS-CoV-2 antigen on a functionalized pharyngeal swab prepared in Example 4 of this invention, which is a swab loaded with a network of MOF black phosphorus nanocomposite material. Figure 7 It can be seen that this functionalized sandpaper has a good SERS signal enhancement effect, with a Raman signal intensity of 1266 at 1066 cm⁻¹.

[0058] Example 5

[0059] This disclosure provides a method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection in this embodiment, including the following steps:

[0060] Step (1): Take a small amount of lumpy black phosphorus (50-100 mg) and grind it into black phosphorus powder in a glove box. Add the black phosphorus powder (30 mg) and polyvinylpyrrolidone (150 mg) to a certain amount of anhydrous ethanol solution (30 mL) and sonicate at 200 W for 4 hours under ice bath conditions to obtain a brown dispersion. Centrifuge at 2000 pm for 15 minutes to remove the lumpy black phosphorus that has not been completely broken up, and then centrifuge at 10000 pm for 10 minutes to obtain a precipitate. After washing with methanol three times and centrifuging, collect the precipitate and redisperse it in methanol solution (6 mL) to obtain a black phosphorus flake dispersion.

[0061] Step (2): Take a small amount of the black phosphorus tablet dispersion (200 μL) prepared in step (1) and add it dropwise to a pharyngeal swab until it just wets the foam pores on the surface of the swab. Let it stand at room temperature. After the swab dries, add cobalt nitrate methanol hexahydrate solution (200 μL in total, 6 mg / mL) in four separate drops. Let it stand at room temperature and dry. Then add 2-methylimidazole methanol solution (80 μL in total, 6 mg / mL) twice. Place the dried swab in cobalt nitrate methanol hexahydrate solution (6 mg / mL) and wash it gently to rinse off any unreacted 2-methylimidazole on the surface of the swab. After wetting the swab, immediately remove it and place it in a petri dish. Let it stand at room temperature to dry, and you will get the functionalized pharyngeal swab of the mesh MOF black phosphorus nanocomposite material.

[0062] In this embodiment, a functionalized pharyngeal swab sampling swab loaded with a mesh MOF black phosphorus nanocomposite material was prepared. This material consists of two parts: the pharyngeal swab swab and sheet-like black phosphorus coated with a mesh MOF. The sheet-like black phosphorus coated with the mesh MOF is applied to the tip of the pharyngeal swab sampling swab. The purchased pharyngeal swab sampling tip has a foam pore density of 300 ppi, a mesh MOF pore size of 300-500 nm, a surface with numerous nanoscale interstitial structures, and a black phosphorus sheet diameter of 1000-2000 nm.

[0063] Figure 8 This is a scanning electron microscope image of a functionalized pharyngeal swab sampling swab containing the mesh-like MOF black phosphorus nanocomposite material prepared in Example 5 of this invention; from Figure 6 It can be seen that the network MOF grows uniformly on the black phosphorus sheet.

[0064] Figure 9 The image shows the Raman spectral detection results of the SARS-CoV-2 antigen on a functionalized pharyngeal swab prepared in Example 5 of this invention, which is a swab loaded with a network of MOF black phosphorus nanocomposite material. Figure 7 It can be seen that this functionalized sandpaper has a good SERS signal enhancement effect, with a Raman signal intensity of 1355 at 1066 cm⁻¹.

[0065] The above description is merely a preferred embodiment of this disclosure and is not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

[0066] While the specific embodiments of this disclosure have been described above in conjunction with the accompanying drawings, this is not intended to limit the scope of protection of this disclosure. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without creative effort based on the technical solutions of this disclosure are still within the scope of protection of this disclosure.

Claims

1. A method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection, characterized in that, include: Step (1): Take a small amount of lumpy black phosphorus and grind it into powder in a glove box. Then weigh the black phosphorus powder and polyvinylpyrrolidone and add them to anhydrous ethanol solution. Place them in an ultrasonic bath for ultrasonic peeling to obtain a brown dispersion. Centrifuge to remove the unpeeled lumpy black phosphorus. Centrifuge the upper dispersion to obtain polyvinylpyrrolidone modified black phosphorus flakes. Wash with methanol and collect the precipitate. Then redisperse it in methanol solution to obtain black phosphorus flake dispersion and store it in a refrigerator. Step (2): Take a small amount of the black phosphorus tablet dispersion prepared in step (1) and drop it onto the pharyngeal swab to moisten the foam pores on the surface of the swab. Let it stand at room temperature. After the swab dries, add cobalt nitrate hexahydrate methanol solution dropwise and let it stand at room temperature. After it dries, add 2-methylimidazole methanol solution dropwise and then wash it. Place it in a petri dish and let it dry at room temperature to obtain a functionalized pharyngeal swab of mesh MOF black phosphorus nanocomposite material. The lumpy black phosphorus is 50-100 mg; the polyvinylpyrrolidone is 90-150 mg; the cobalt nitrate hexahydrate methanol solution is 6 mg / mL, totaling 100-200 μl; and the 2-methylimidazolium methanol solution is 6 mg / mL, totaling 40-80 μl.

2. The method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection as described in claim 1, characterized in that, 30 ml of the anhydrous ethanol solution.

3. The method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection as described in claim 1, characterized in that, 6 ml of methanol solution.

4. The method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection as described in claim 1, characterized in that, The cobalt nitrate hexahydrate methanol solution was added dropwise in four batches.

5. The method for preparing a throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection as described in claim 1, characterized in that, The 2-methylimidazolium methanol solution was added dropwise in two steps.

6. The throat swab loaded with a network MOF black phosphorus nanocomposite material for SERS detection prepared by the method described in claim 1, characterized in that, The pharyngeal swab consists of two parts: a pharyngeal swab sampling swab and a sheet of black phosphorus coated with a mesh MOF; the sheet of black phosphorus coated with the mesh MOF is applied to the head of the pharyngeal swab sampling swab.

7. The throat swab as described in claim 6, characterized in that, The foam pore density of the pharyngeal swab sampling swab head is 300ppi.

8. The throat swab as described in claim 6, characterized in that, The mesh MOF has a pore size of 200-300 nanometers and a nanometer-level gap structure on its surface.

9. The throat swab as described in claim 6, characterized in that, The diameter of the flaky black phosphorus is 1000-5000 nanometers.

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