A chromatography column device for a glycated hemoglobin analyzer

Abstract

A kind of glycated hemoglobin analyzer chromatography column device, involve medical detection equipment technical field, solve the technical deficiencies of existing device complex structure, chromatography column assembly disassembly inconvenient, seal is not strict, maintenance difficulty, detection is not accurate, the technical scheme that is used includes: heating seat component and the clamping fixing mechanism that can be detachably fixed on it, heating seat component is equipped with first liquid path and the heating element for heating the liquid in first liquid path;Chromatography column cavity is equipped on the clamping fixing mechanism, its operating end can be elastically pushed out axially in chromatography column cavity, and third liquid path is equipped on its operating end;Second liquid path is equipped on chromatography column component, under the elastic pressure of the operating end of clamping fixing mechanism, it is fixed in chromatography column cavity and is connected with the liquid outlet end of first liquid path.This application designs heating seat component and clamping fixing device as integrated modular structure, simple structure, easy to maintain, the elastic clamping fixation mode of chromatography column component also facilitates disassembly, strong adaptability, not easy to leak.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical detection equipment, more particularly to a chromatographic column device for a glycated hemoglobin analyzer. BACKGROUND

[0002] Glycated hemoglobin HbA1c is formed by the slow and irreversible non-enzymatic reaction of certain special molecular sites of hemoglobin A component and glucose, and when the glucose concentration in the blood is high, the content of glycated hemoglobin synthesized by the human body will also be relatively high. Therefore, the content of glycated hemoglobin in the blood sample can reflect the stage blood glucose level of the subject, and thus, glycated hemoglobin HbA1c is commonly used as an effective detection index for diabetes screening, diagnosis, blood glucose control, and efficacy evaluation.

[0003] Microcolumn ion exchange chromatography is currently the mainstream detection method of glycated hemoglobin analyzers. The chromatographic column device is used as a carrier to separate HbA1c in the blood sample through gradient elution technology, and then the content is calibrated through chromatographic analysis. In actual use, the chromatographic column and the reagent need to be heated by a heating device, and the chromatographic column needs to be replaced after a period of use. Therefore, the glycated hemoglobin analyzer not only needs to meet the heating requirement, but also needs to realize the convenient disassembly and replacement of the chromatographic column.

[0004] The existing chromatographic column device applied to the glycated hemoglobin analyzer has a too complex structure, a high failure rate, and occupies a large internal space of the analyzer due to the complex structure, which limits the structure design and size of the chromatographic column assembly, is not conducive to the disassembly and assembly of the chromatographic column assembly, brings difficulties to the later maintenance, and is inconvenient for users to use. Moreover, the chromatographic column assembly in the existing analyzer is mostly detachably installed in the chromatographic column cavity in a hard locking and fixing manner (such as a threaded fastening manner), which can ensure the reliability of the fixed assembly of the chromatographic column assembly. However, different batches of chromatographic column assemblies may have slight differences in size, which may cause the chromatographic column assembly with size deviation to leak liquid and affect the normal use of the analyzer and the accuracy of the detection result due to the loose sealing of the liquid inlet channel.

[0005] Therefore, it is necessary to improve the structure of the existing chromatographic column device to overcome the above problems. SUMMARY

[0006] In summary, the purpose of the present application is to solve the technical problems of the unreasonable structure design, complex structure, high failure rate, inconvenient disassembly and assembly, loose locking and sealing, difficult later maintenance, liquid leakage, and inaccurate detection of the existing chromatographic column device of the glycated hemoglobin analyzer, and to provide an improved chromatographic column device of the glycated hemoglobin analyzer with a simpler and more reasonable structure design, more convenient replacement operation of the chromatographic column, easier maintenance, and less liquid leakage.

[0007] To solve the technical problems of the present application, the technical solution adopted is a chromatographic column device for a glycated hemoglobin analyzer, characterized in that it comprises:

[0008] A heating seat assembly is provided with a first liquid path for liquid entry and a heating body for heating the liquid in the first liquid path;

[0009] A clamping and fixing mechanism is detachably fixed to the heating seat assembly, and is provided with a chromatographic column cavity axially communicated to the liquid outlet end of the first liquid path, and the action end of the clamping and fixing mechanism can be elastically pushed out axially into the chromatographic column cavity, and the action end is provided with a third liquid path coaxial with the liquid outlet end of the first liquid path;

[0010] The chromatographic column assembly is provided with a second liquid path on its axis, and is axially fixed in the chromatographic column cavity and abuts against the liquid outlet end of the first liquid path under the elastic pressure of the action end of the clamping and fixing mechanism, so that the third liquid path, the second liquid path and the first liquid path are in sealed butt joint communication.

[0011] Further, the clamping and fixing mechanism comprises:

[0012] A mounting seat comprises a lower cylinder portion and an upper cylinder portion coaxially connected, and a first jack rod through hole axially communicating the inner cavities of the lower cylinder portion and the upper cylinder portion; the bottom end of the lower cylinder portion is provided with an opening, and the inner cavity constitutes the chromatographic column cavity; at least one guide groove extending around the axis of the upper cylinder portion is formed in the cylinder wall of the upper cylinder portion;

[0013] A pressure base is movably arranged in the upper cylinder portion, and at least one connecting column is radially connected to the side wall of the pressure base and extends out of the guide groove; a second jack rod through hole coaxial with the first jack rod through hole is formed in the axis of the pressure base;

[0014] A locking jack rod is movably inserted into the second jack rod through hole through an elastic connection structure, and the third liquid path is formed in the axis of the locking jack rod; under normal conditions, the first end of the locking jack rod can be axially extended out of the lower end of the pressure base through the second jack rod through hole under the elastic force of the elastic connection structure;

[0015] A knob is movably sleeved on the upper cylinder portion and fixedly connected to the pressure base through the connecting column;

[0016] When the knob is moved downward to its lower limit position under the limiting action of the connecting column and the guide groove, the pressure base drives the locking jack rod to elastically abut against the chromatographic column assembly through the first jack rod through hole; when the knob is moved upward to its upper limit position, the pressure base drives the locking jack rod to axially separate from the chromatographic column assembly.

[0017] Further, two center-symmetrical guiding grooves extending along the axis of the upper cylinder part are formed on the cylinder wall of the upper cylinder part, and two connecting columns extending through the guiding grooves are connected to the side wall of the pressure base.

[0018] Further, vertical anti-skid stripes are evenly distributed on the side wall of the knob.

[0019] Further, the elastic connecting structure comprises:

[0020] a spring cavity axially arranged in the pressure base, and the second top rod through hole passes through the axis of the spring cavity and communicates with the spring cavity;

[0021] a anti-dropping part radially arranged on the locking top rod and movably arranged in the spring cavity, and the outer diameter of the anti-dropping part is greater than the inner diameter of the second top rod through hole;

[0022] a pressure spring elastically arranged in the spring cavity and movably sleeved on the locking top rod, and in a normal state, the lower end of the pressure spring elastically abuts against the anti-dropping part, and the upper end of the pressure spring elastically abuts against the upper end of the spring cavity, so as to elastically push the anti-dropping part to the lower end of the spring cavity, and drive the first end of the locking top rod to automatically elastically extend out of the lower end of the pressure base through the second top rod through hole.

[0023] Further, the guiding groove comprises:

[0024] an arc-shaped groove segment extending along the axis of the upper cylinder part, for guiding the connecting column to drive the pressure base to axially rotate and move to the lower end of the upper cylinder part;

[0025] a straight groove segment located at the upper part of the arc-shaped groove segment and communicating with the arc-shaped groove segment, extending axially along the axis of the upper cylinder part, for guiding the connecting column to drive the pressure base to axially linearly move to the upper end of the upper cylinder part;

[0026] a circumferential groove segment located at one side of the upper part of the straight groove segment and communicating with the straight groove segment, extending along the tangent direction of the outer periphery of the upper cylinder part, for movably clamping the connecting column to limit the pressure base at the upper limit position.

[0027] Further, the clamping and fixing mechanism further comprises:

[0028] an assembly opening formed on the front side of the lower cylinder part to form an opening at the front part of the chromatography column cavity, for disassembling and assembling the chromatography column assembly;

[0029] a protective cover detachably and fixedly connected to the front side of the lower cylinder part to block the assembly opening, and a limiting part extending into the chromatography column cavity to limit the chromatography column assembly is arranged on the inner side of the protective cover, and a handle for hand holding is arranged on the outer side of the protective cover.

[0030] Further, the heating seat assembly comprises:

[0031] The heat seat is a cuboid structure made of hard heat-conducting material, and a liquid channel groove and a heating cavity on one side of the liquid channel groove are arranged on the heat seat.

[0032] The inlet pipe is arranged in the liquid channel groove, and first inlet and outlet ends in communication with the inner cavity thereof are arranged at the two ends of the inlet pipe and are clamped to the outer ends of the liquid channel groove at the two ends.

[0033] The heating body is fixedly connected to the heat seat and located in the heating cavity, and the first outlet end is located in the chromatographic column cavity and is axially and sealingly clamped to the lower end of the chromatographic column assembly.

[0034] Further, the heating seat assembly further comprises:

[0035] The temperature sensor is fixedly connected to the heat seat and is used for detecting the temperature of the heat seat.

[0036] Further, the heating seat assembly further comprises:

[0037] The heat seat cover is a cover structure made of hard heat-insulating material, and a fixed cover is arranged on the heat seat, and a heat dissipation opening corresponding to the heating cavity and an empty slot exposing the clamping and fixing mechanism are arranged on the heat seat cover.

[0038] The present application has the following advantages:

[0039] 1. The device of the present application preheats the liquid in the first liquid channel that is about to enter the chromatographic column assembly through the heating body on the heating seat assembly, and the chromatographic column cavity is connected to the heating seat assembly in a contact manner through the clamping and fixing mechanism, which is conducive to maintaining the temperature in the chromatographic column cavity. Compared with the existing design, the structure is simpler, the design is more reasonable, the heating efficiency is higher, the effect is better, the elution efficiency of the chromatographic column assembly is significantly improved, and the glycosylated hemoglobin in the blood sample is obtained.

[0040] 2. The device of the present application elastically clamps and fixes the chromatographic column assembly in the chromatographic column cavity by the action end of the clamping and fixing mechanism, which can realize the convenient disassembly and assembly of the chromatographic column assembly. The self-adaptive sealing butt joint communication between the chromatographic column assembly and the outlet end of the first liquid channel can be realized by the elastic ejection of the action end of the clamping and fixing mechanism. Compared with the existing hard locking and fixing method of the chromatographic column assembly, the device of the present application solves the problem that different batches of chromatographic column assemblies cannot be perfectly butt jointed with the outlet end of the first liquid channel due to slight differences in size, and has higher adaptability and reliability. Moreover, the elastic sealing butt joint method is also conducive to the stress distribution between the contact peripheries of the second liquid channel and the outlet end of the first liquid channel, improves the sealing effect of the butt joint, avoids the leakage phenomenon, and ensures the accuracy of the analyzer detection.

[0041] 3、The device has reasonable design, simple structure and convenient use, the clamping and fixing mechanism and the heating seat assembly are detachably connected as a whole, so that the two constitute a modular structure which can be conveniently disassembled, replaced or maintained, the later maintenance treatment is facilitated, the overall replacement of the modular structure can be realized, and the service life of the glycated hemoglobin analyzer is prolonged. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a schematic diagram of the overall structure of the device of the application;

[0043] Figure 2 It is a schematic diagram of the overall structure of the device of the application; Figure 1

[0044] Figure 3 It is a schematic diagram of the overall structure of the device of the application; Figure 2

[0045] Figure 4 It is a schematic diagram of the overall structure of the device of the application;

[0046] Figure 5 It is a schematic diagram of the structure of the clamping and fixing mechanism hidden by the protective cover of the device of the application;

[0047] Figure 6 It is a schematic diagram of the structure of the clamping and fixing mechanism hidden by the protective cover of the device of the application; Figure 1

[0048] Figure 7 It is a schematic diagram of the structure of the clamping and fixing mechanism hidden by the protective cover of the device of the application; Figure 2

[0049] Figure 8 It is a schematic diagram of the overall structure of the device of the application;

[0050] ​​​​In the figure: 1. heating seat assembly, 11. heat seat, 111. liquid channel, 112. heating cavity, 113. heat seat body, 114. heat seat guard, 115. first fixed through hole, 116. fixed groove, 12. liquid inlet pipe, 13. first liquid inlet end, 14. first liquid outlet end, 15. temperature sensor, 16. heat seat cover, 161. heat dissipation port, 162. clearance groove, 2. clamping and fixing mechanism, 21. mounting seat, 211. lower cylinder part, 212. upper cylinder part, 213. first top rod through hole, 214. first fixed screw hole, 215. guide groove, 2151. arc groove section, 2152. straight groove section, 2153. circumferential groove section, 22. pressure base, 221. second top rod through hole, 222. connecting hole, 223. inner groove, 224. second fixed screw hole, 225. cover plate, 23. locking top rod, 231. anti-falling part, 232. top rod part, 233. connecting pipe part, 24. knob, 241. clamping hole, 242. vertical anti-slip stripe, 25. connecting column, 26. spring cavity, 27. pressure spring, 28. assembly opening, 29. protective cover, 291. limiting part, 292. handle, 3. chromatography column assembly, 31. lower column section, 32. upper column section, 33. chromatography column liquid inlet hole, 34. plunger sheet, 35. chromatography column liquid outlet hole, 4. heating body, 5. chromatography column cavity, 6. third liquid channel. DETAILED DESCRIPTION

[0051] The structure of the present application will be further described below in combination with the drawings and preferred specific embodiments of the present application.

[0052] Reference Figures 1 to 3 As shown in the figure, the chromatography column device of the present application for analyzing glycated hemoglobin is characterized in that it comprises a heating seat assembly 1, a clamping and fixing mechanism 2, and a chromatography column assembly 3.

[0053] Specifically, the heating seat assembly 1 is provided with a first liquid channel for liquid to enter and a heating body 4 for heating the liquid in the first liquid channel.

[0054] Specifically, the clamping and fixing mechanism 2 is detachably fixedly connected to the heating seat assembly 1, and the chromatography column cavity 5 axially connected to the liquid outlet end of the first liquid channel is arranged on the clamping and fixing mechanism 2. The action end of the clamping and fixing mechanism 2 can be elastically pushed out axially into the chromatography column cavity 5, and the action end is provided with a third liquid channel 6 coaxial with the liquid outlet end of the first liquid channel.

[0055] Specifically, the chromatography column assembly 3 is provided with a second liquid channel on its axis. In actual assembly, the chromatography column assembly 3 is axially clamped and fixed in the chromatography column cavity 5 under the elastic pressure of the action end of the clamping and fixing mechanism 2 and abuts against the liquid outlet end of the first liquid channel, so that the third liquid channel 6, the second liquid channel, and the first liquid channel are in sealed butt joint communication.

[0056] The device has the following beneficial effects.

[0057] Firstly, the device preheats the liquid in the first liquid path through the heating body 4 on the heating seat assembly 1, and the chromatography column cavity 5 is connected with the heating seat assembly 1 in a contact manner through the clamping fixing mechanism 2, which is beneficial to maintain the temperature in the chromatography column cavity 5. Compared with the existing design, the structure is simpler, the design is more reasonable, the heating efficiency is higher, the effect is better, and the elution efficiency of the chromatography column assembly 3 is significantly improved, which is beneficial to the acquisition of glycosylated hemoglobin in the blood sample.

[0058] Meanwhile, the device elastically clamps and fixes the chromatography column assembly 3 in the chromatography column cavity 5 through the action end of the clamping fixing mechanism 2, which can realize the convenient disassembly of the chromatography column assembly 3, and can also realize the self-adaptive sealing butt joint communication between the chromatography column assembly 3 and the liquid outlet end of the first liquid path through the elastic ejection feature of the action end of the clamping fixing mechanism 2. Compared with the existing hard locking method of fixing the chromatography column assembly 3, the device solves the problem that different batches of chromatography column assemblies 3 cannot be perfectly butt jointed with the liquid outlet end of the first liquid path due to slight differences in size, and has stronger adaptability and higher reliability. Moreover, the elastic sealing butt joint method is also beneficial to the stress distribution between the contact periphery between the second liquid path and the liquid outlet end of the first liquid path, improves the sealing effect of the butt joint, avoids the leakage phenomenon, and ensures the accuracy of the analyzer detection.

[0059] In addition, the device has reasonable design and simple structure, and is convenient to use. The clamping fixing mechanism 2 and the heating seat assembly 1 are detachably connected as a whole, so that they form a modular structure which can be conveniently disassembled, replaced or repaired, facilitating the later maintenance treatment, and the overall replacement of the modular structure can be realized, thereby prolonging the service life of the glycosylated hemoglobin analyzer.

[0060] As a further improved technical solution adopted by the device to solve the technical problems proposed by the device, the following technical features are also included.

[0061] The heating body 4 is an electric heating heating body 4, preferably using a heating resistor with adjustable resistance to heat. Of course, in other embodiments, the heating body 4 can also use a graphene heating body 4 or a ceramic heating body 4, etc.

[0062] Preferably, referring to Figure 4 The chromatography column assembly 3 of the device includes a lower column segment 31 in a hollow cylindrical structure and an upper column segment 32 which is detachably axially fixed and connected to the lower column segment 31.

[0063] Specifically, the upper end of the lower column segment 31 is provided with an opening, the bottom of which is axially sealed and connected to the liquid outlet end of the first liquid path, and the bottom axis position is provided with a chromatographic column liquid inlet hole 33 communicating with the inner cavity, and the inner cavity bottom is provided with a plunger sheet 34 for supporting ion exchange resin.

[0064] Specifically, the upper column segment 32 is a cylindrical structure with openings at both upper and lower ends and a blocked middle part, the lower part of which is axially sealed and connected to the lower column segment 31 and seals the inner cavity thereof, so that the inner cavities of the two form a cavity for accommodating ion exchange resin. The upper part of the upper column segment 32 is axially sealed and connected to the action end of the clamping and fixing mechanism 2, and the axis of the blocked middle part is provided with a chromatographic column liquid outlet hole 35 corresponding to the chromatographic column liquid inlet hole 33 and the third liquid path 6. The lower column segment 31, the lower part of the upper column segment 32, and the chromatographic column liquid inlet hole 33 and the chromatographic column liquid outlet hole 35 together form the second liquid path.

[0065] The chromatographic column assembly 3 of the device has a simple structure, is easy to disassemble and assemble, is convenient for replacing ion exchange resin, and is convenient for users to use. Moreover, the structure design of the lower column segment 31 and the upper column segment 32 facilitates the axial sealing butt joint of the chromatographic column assembly 3 to the liquid outlet end of the first liquid path and the action end of the clamping and fixing mechanism 2, thereby ensuring the reliability of the sealed butt joint communication of the first liquid path, the second liquid path, and the third liquid path 6.

[0066] Further, referring to Figures 5 to 7 The clamping and fixing mechanism 2 of the device includes a mounting seat 21, a pressure base 22, a locking top rod 23, and a knob 24.

[0067] Specifically, as shown in Figure 6 The mounting seat 21 includes a lower cylindrical part 211 and an upper cylindrical part 212 connected coaxially, both of which are hollow cylindrical structures, and a first top rod through hole 213 vertically and through the axis position of the connecting part of the two is provided to communicate the inner cavities of the two.

[0068] Specifically, the bottom end of the lower cylindrical part 211 is provided with an opening that can cover the liquid outlet end of the first liquid path, and the inner cavity forms a chromatographic column cavity 5 for accommodating the chromatographic column assembly 3. A plurality of first fixing screw holes 214 are provided on the end face of the bottom end opening of the lower cylindrical part 211. In actual assembly, the mounting seat 21 is vertically and threadedly fixedly connected to the heating seat assembly 1 through the first fixing screw holes 214, and the chromatographic column cavity 5 covers the liquid outlet end of the first liquid path, so that the liquid outlet end of the first liquid path and the first top rod through hole 213 are on the same axis.

[0069] Specifically, the upper cylindrical part 212 is a hollow cylindrical structure with an opening at the upper end, and at least one guide groove 215 extending around the axis is provided on the cylindrical wall.

[0070] Specifically, as shown in Figures 6 to 7As shown, the pressure base 22 is a cylindrical structure movably arranged in the upper cylinder portion 212, and the outer diameter thereof is preferably matched with the inner diameter of the upper cylinder portion 212, and the axial length thereof is preferably matched with the height of the inner diameter of the upper cylinder portion 212. At least one connecting column 25 is radially connected to the middle position of the sidewall of the pressure base 22 and extends out of the guide groove 215, and a second top rod through hole 221 coaxial with the first top rod through hole 213 on the mounting base 21 is provided through the axis of the pressure base 22.

[0071] Specifically, the locking top rod 23 is a rod structure movably inserted into the second top rod through hole 221 of the pressure base 22 through an elastic connecting structure, and the third liquid path 6 is provided through the axis of the locking top rod 23.

[0072] Under normal circumstances, the first end (i.e. the lower end) of the locking top rod 23 can axially extend out of the lower end of the pressure base 22 through the second top rod through hole 221 under the elastic force of the elastic connecting structure.

[0073] Specifically, the knob 24 is a sleeve structure movably sleeved on the upper cylinder portion 212 and fixedly connected with the pressure base 22 through the connecting column 25.

[0074] Preferably, the sidewall of the lower part of the knob 24 is provided with a clamping hole 241 corresponding to the clamping of the connecting column 25, and the opening size of the clamping hole 241 is matched with the outer diameter of the outer end of the connecting column 25, and the sidewall of the middle part of the pressure base 22 is provided with a connecting hole 222 corresponding to the fixation of the inner end of the connecting column 25. In actual assembly, the pressure base 22 is movably inserted into the upper cylinder portion 212 and the connecting holes 222 thereon are correspondingly matched with the guide grooves 215, then the knob 24 is sleeved on the upper cylinder portion 212 and the clamping holes 241 thereon are correspondingly matched with the connecting holes 222 inside the guide grooves 215, and finally the connecting column 25 is fixedly connected in the connecting holes 222 from the outside to the inside through the clamping holes 241 and the guide grooves 215, so that the knob 24 is fixedly connected with the pressure base 22 through the radially fixed connecting column 25, and the knob 24 and the pressure base 22 are prevented from axially separating from the upper cylinder portion 212 under the limiting of the connecting column 25 and the guide groove 215.

[0075] Of course, in other embodiments, the connecting column 25 can also be designed as a screw rod structure or a bolt structure, as long as it can fixedly connect the knob 24 with the pressure base 22 through the guide groove 215.

[0076] The movement of the connecting column 25 is limited by the guide groove 215, when the knob 24 is rotated downward by the external force of the user to the lower limit of its movement, the pressure base 22 is driven to rotate downward synchronously by the connecting column 25, so that the first end of the locking jack 23 elastically connected to the pressure base 22 is elastically tightly abuts the chromatographic column assembly 3 through the first jack through hole 213, thereby elastically pushing the chromatographic column assembly 3 downward to the liquid outlet end of the first liquid path and making the two seal and dock.

[0077] When the knob 24 is moved upward by the external force of the user to the upper limit of its movement, the pressure base 22 is driven to move upward synchronously by the connecting column 25, so that the first end of the locking jack 23 is axially separated from the chromatographic column assembly 3, thereby releasing the axial pressure of the chromatographic column assembly 3, so as to facilitate the user to disassemble the chromatographic column assembly 3.

[0078] The clamping and fixing mechanism 2 of the device has the advantages of simple structure, reasonable design, compact layout, few components involved, low failure rate, simple operation, convenience, and the like, the axial clamping or separation between the locking jack 23 and the chromatographic column assembly 3 is controlled by the knob 24, which is beneficial to the quick and convenient disassembly and assembly of the chromatographic column assembly 3.

[0079] Further, referring to Figures 6 to 7 The upper barrel portion 212 of the device is provided with two center-symmetric guide grooves 215 extending around the axis on the barrel wall, and the side wall of the pressure base 22 is correspondingly connected with two connecting columns 25 extending through the guide grooves 215.

[0080] The two center-symmetric guide grooves 215 provided on the upper barrel portion 212 can improve the reliability of the fixed connection between the knob 24 and the pressure base 22, and can prevent the pressure base 22 from affecting the axial elastic pushing of the locking jack 23 to the chromatographic column assembly 3 due to unilateral force.

[0081] Further, referring to Figure 5 The upper barrel portion 212 of the device is provided with two center-symmetric guide grooves 215 extending around the axis on the barrel wall, and the side wall of the pressure base 22 is correspondingly connected with two connecting columns 25 extending through the guide grooves 215.

[0082] Further, referring to Figures 6 to 8 The elastic connection structure of the clamping and fixing mechanism 2 includes a spring cavity 26 (as shown in Figure 8 ), a anti-disengagement portion 231 and a pressure spring 27 (as shown in Figure 6 ).

[0083] Specifically, the spring cavity 26 is axially arranged in the pressure base 22, and the second top rod through hole 221 penetrates the axis of the spring cavity 26 and communicates with the spring cavity 26.

[0084] Specifically, the anti-disengagement part 231 is a disc structure, is radially fixed at the middle position of the locking top rod 23, and is movably arranged in the spring cavity 26, and the outer peripheral size of the anti-disengagement part 231 is greater than the inner diameter of the second top rod through hole 221, so that the locking top rod 23 is prevented from being axially disengaged from the pressure base 22, and the reliability of the elastic connection between the locking top rod 23 and the pressure base 22 is ensured.

[0085] Specifically, the pressure spring 27 is elastically arranged in the spring cavity 26 and movably sleeved on the locking top rod 23, and in a normal state, the lower end of the pressure spring 27 elastically abuts against the anti-disengagement part 231, and the upper end of the pressure spring 27 elastically abuts against the upper end of the spring cavity 26, so as to elastically push the anti-disengagement part 231 to the lower end of the spring cavity 26, and drive the first end of the locking top rod 23 to automatically elastically extend outward from the lower end of the pressure base 22 through the second top rod through hole 221.

[0086] The elastic connection structure of the clamping and fixing mechanism 2 has few components, the spring cavity 26 is arranged in the pressure base 22, the layout of the elastic connection structure is more compact, the size of the pressure base 22 is reduced, and the overall miniaturization and intensification design of the device are facilitated.

[0087] In actual use, the knob 24 is axially reciprocated by the guide groove 215, and the axial pressure loading and unloading of the locking top rod 23 on the chromatographic column assembly 3 are realized under the elastic force of the pressure spring 27. The axial tension value generated by the pressure spring 27 is relatively stable, the fluid interface pressure at both ends of the chromatographic column liquid inlet hole 33 and the chromatographic column liquid outlet hole 35 is stable in the clamping and fixing state of the chromatographic column assembly 3, and the sealing is reliable.

[0088] Preferably, referring to Figures 6 to 8 The pressure base 22 of the device is provided with an inner recess 223 which is recessed upward along the bottom end face of the pressure base 22, a plurality of second fixing screw holes 224 are uniformly distributed on the bottom end edge of the pressure base 22, the bottom of the pressure base 22 is detachably connected to a cover plate 225 for plugging the inner recess 223 through the second fixing screw holes 224 and a screw rod, and the second top rod through hole 221 axially penetrates the inner recess 223 and the cover plate 225.

[0089] During actual assembly, first insert the pressure spring 27 through the tail end (upper part) of the locking push rod 23, insert the cover plate 225 through the head end (lower part) of the second push rod through hole 221, and then insert the locking push rod 23 into the inner groove 223 from bottom to top, press the cover plate 225 to push the locking push rod 23 upward, so that the upper end of the pressure spring 27 abuts against the top of the inner groove 223 (the upper end of the spring cavity 26) and the pressure spring 27 is partially compressed. After the cover plate 225 abuts against the lower end of the pressure base 22, it is fixedly connected to the pressure base 22 as a whole through a screw, so that the pressure spring 27 is in a compressed state and drives the head end of the locking push rod 23 to elastically eject outward from the lower end of the pressure base 22 through the second push rod through hole 221.

[0090] After the cover plate 225 is installed at the bottom of the pressure base 22 , the inner groove 223 and the cover plate 225 together constitute the spring chamber. The second push rod through hole 221 is located on the axis of the pressure base 22 and passes through the inner groove 223 and the cover plate 225 .

[0091] The structural design of the pressure base 22 in this embodiment is only a preferred embodiment of the present invention and is not intended to limit the technical solution of the present invention. In other embodiments, the cover plate 225 can be designed to have a structure similar to a bottle cap and be screw-fastened to the pressure base 22 in a screw-fastened manner.

[0092] Further, refer to Figure 6 As shown, the guide groove 215 of the clamping and fixing mechanism 2 of the present invention is a three-section structure, including an arcuate groove section 2151 , a straight groove section 2152 and a circumferential groove section 2153 .

[0093] Specifically, the arc-shaped groove section 2151 extends around the axis of the upper cylinder portion 212 and is used to guide the connecting column 25 to drive the pressure base 22 to axially rotate and move toward the lower end of the upper cylinder portion 212.

[0094] Specifically, the straight groove section 2152 is located above the arc-shaped groove section 2151 and is connected thereto. The straight groove section 2152 extends axially along the axis of the upper cylinder portion 212 and is used to guide the connecting column 25 to drive the pressure base 22 to move axially in a straight line toward the upper end of the upper cylinder portion 212.

[0095] Specifically, the circumferential groove section 2153 is located on the upper side of the straight groove section 2152 and is connected thereto. The circumferential groove section 2153 extends along the tangential direction of the outer periphery of the upper cylinder portion 212 and is used to movably engage the connecting column 25 to limit the pressure base 22 to its upper limit position.

[0096] The guiding groove 215 of the device is designed as follows: the arc-shaped groove section 2151 guides the rotating movement of the connecting column 25 and the pressure base 22 to the lower end of the upper cylinder 212, thereby driving the locking top rod 23 to elastically press against the chromatographic column assembly 3 through the first top rod through hole 213 to clamp and fix the chromatographic column assembly 3 in the chromatographic column cavity 5; the straight groove section 2152 guides the linear movement of the connecting column 25 and the pressure base 22 to the upper end of the upper cylinder 212, thereby driving the locking top rod 23 to axially separate from the chromatographic column assembly 3 to release the axial elastic pressure of the chromatographic column assembly 3; the circumferential groove section 2153 movably clamps the connecting column 25 to limit the pressure base 22 and the locking top rod 23 at the upper limit position, thereby preventing the connecting column 25 from falling along the straight groove section 2152 to drive the pressure base 22 and the locking top rod 23 to move downward during the disassembly and assembly of the chromatographic column assembly 3, and avoiding the interference of the first end of the locking top rod 23 with the disassembly and assembly of the chromatographic column assembly 3.

[0097] Preferably, as shown in Figures 6 to 8 The locking top rod 23 of the device comprises a top rod part 232 and a connecting pipe part 233 axially connected to the top rod part 232, and a through hole is arranged on the axis of the top rod part 232 and the connecting pipe part 233 and communicates with each other, and the through hole constitutes the third liquid path 6 after sealing butt joint communication.

[0098] The anti-disengagement part 231 is radially fixed to the upper position of the top rod part 232, and in actual use, the liquid in the second liquid path of the chromatographic column assembly 3 is guided outwards through the connecting pipe part 233.

[0099] Further, as shown in Figure 2 、 Figure 3 、 Figure 5 and Figure 8 The clamping and fixing mechanism 2 of the device further comprises an assembly opening 28 and a protective cover 29.

[0100] Specifically, the assembly opening 28 is arranged on the front side of the lower cylinder part 211 to form an opening in the front part of the chromatographic column cavity 5, and is used for the disassembly and assembly of the chromatographic column assembly 3.

[0101] Specifically, as shown in Figure 2 The protective cover 29 is detachably fixedly connected to the front side of the lower cylinder part 211 to block the assembly opening 28, the inner side of the protective cover 29 is provided with a limiting part 291 extending into the chromatographic column cavity 5 to limit the chromatographic column assembly 3, and the outer side of the protective cover 29 is provided with a handle 292 for hand holding.

[0102] The device exposes the chromatographic column cavity 5 to the outside through the assembly opening 28, so that the user can conveniently disassemble and assemble the chromatographic column assembly 3 through the assembly opening 28. Meanwhile, the device blocks the assembly opening 28 through the protective cover 29 detachably connected to the front side of the lower barrel portion 211, and limits the chromatographic column assembly 3 in the chromatographic column cavity 5 through the limiting portion 291, thereby playing a safety protection role, making the clamping and fixing of the chromatographic column assembly 3 more stable and reliable, and effectively preventing external objects from knocking or scratching the chromatographic column assembly 3. Meanwhile, the protective cover 29 can also effectively block the chromatographic column cavity 5, thereby playing a good heat preservation effect and being beneficial to the gradient elution.

[0103] Further, referring to FIGS. 1-3, Figure 2 and Figure 3 the device includes the heating seat assembly 1, which includes the heat seat 11 and the liquid inlet pipe 12.

[0104] Specifically, the heat seat 11 is a cube structure made of hard heat-conducting material, and is provided with the liquid channel groove 111 penetrating through the left side end face and the upper end face of the heat seat 11, and the heating cavity 112 corresponding to the rear side of the liquid channel groove 111.

[0105] Specifically, the liquid inlet pipe 12 is a heat-resistant hose, which is matched and accommodated in the liquid channel groove 111, and is provided with the first liquid inlet end portion 13 and the first liquid outlet end portion 14 respectively clamped to the left end and the upper end of the liquid channel groove 111 and communicating with the inner cavity of the liquid inlet pipe 12, and the liquid inlet pipe 12, the first liquid inlet end portion 13 and the first liquid outlet end portion 14 together constitute a first liquid path for liquid passing through, and the first liquid outlet end portion 14 constitutes the liquid outlet end of the first liquid path.

[0106] The heating body 4 is fixedly connected to the heat seat 11 and located in the heating cavity 112, and the first liquid outlet end portion 14 is located in the chromatographic column cavity 5 and axially and sealingly clamped to the lower end of the chromatographic column assembly 3.

[0107] Specifically, the first liquid outlet end portion 14 is a barrel structure communicating with the liquid inlet pipe 12, and the inner cavity diameter thereof is matched with the outer diameter of the lower column segment 31 of the chromatographic column assembly 3, so that the first liquid outlet end portion 14 can be axially and sealingly clamped to the lower column segment 31 to make the second liquid path sealingly and dockingly communicate with the first liquid path.

[0108] Since the heat seat 11 is made of hard heat-conducting material, the liquid channel groove 111 is arranged in the heat seat 11, and the liquid inlet pipe 12 is embedded in the liquid channel groove 111, so that the heat emitted by the heating body 4 can be directly transmitted to the liquid inlet pipe 12 through the liquid channel groove 111, thereby realizing the heating of the liquid in the liquid inlet pipe 12, and the structure is simple, the heating efficiency is high, and the effect is good.

[0109] Preferably, referring to Figure 3 As shown in the figure, the heat seat 11 of the device of the present application comprises a heat seat body 113 and a heat seat guard 114, the liquid path groove 111 is opened on the front end face of the heat seat body 113, the heating cavity 112 is arranged at the rear end of the heat seat body 113, and the heat seat guard 114 is threadedly fixedly connected to the front side of the heat seat body 113 to block the liquid path groove 111 and limit and protect the liquid inlet pipe 12 in the liquid path groove 111. This kind of structural design facilitates the assembly of the liquid inlet pipe 12, the first liquid inlet end portion 13 and the first liquid outlet end portion 14, and also facilitates the disassembly of the liquid inlet pipe 12 in the later period, facilitating maintenance.

[0110] Specifically, the upper portion of the heat seat body 113 is provided with a first fixed through hole 115 penetrating into the heating cavity 112 and matched with the first fixed screw hole 214, and in actual assembly, the mounting seat 21 is fixedly connected to the heat seat body 113 through the first fixed through hole 115 and the first fixed screw hole 214 by means of a screw rod.

[0111] Further, referring to Figure 3 As shown in the figure, the heat seat assembly 1 of the device of the present application further comprises a temperature sensor 15, which is electrically connected with the main control unit (not shown in the figure) of the analyzer, for detecting the temperature of the heat seat 11 and reporting the temperature sensing data to the main control unit in real time, so that the analyzer can autonomously regulate the heat generation of the heating body 4 according to the feedback temperature value, thereby realizing the control of the temperature of the liquid in the first liquid path.

[0112] Specifically, the rear end of the heat seat body 113 is provided with an inner recessed fixed groove 116 in the heating cavity 112, and the temperature sensor 15 is fixedly connected in the fixed groove 116. In the later period, the heating body 4 and the temperature sensor 15 can be conveniently disassembled through the heating cavity 112, facilitating the later maintenance.

[0113] Further, referring to Figures 2 to 3 As shown in the figure, the heat seat assembly 1 of the device of the present application further comprises a heat seat guard 16, which is a guard type structure made of hard heat insulation material, and is arranged on the heat seat 11 composed of the heat seat body 113 and the heat seat guard 114, and is provided with a heat dissipation opening 161 corresponding to the heating cavity 112 and an empty slot 162 exposing the clamping fixing mechanism 2.

[0114] The heat seat guard 16 of the device of the present application is made of hard heat insulation material, which not only plays a role in protecting the heat seat 11, but also has the effects of heat preservation and anti-scalding. In addition, the arrangement of the heat dissipation opening 161 also facilitates the disassembly of the heating body 4 and the temperature sensor 15 without disassembling the heat seat guard 16, simplifies the operation of maintenance, and the heat dissipation opening 161 is also beneficial to the heat dissipation of the heat seat 11, shortens the waiting time for the heat dissipation of the heat seat 11 during maintenance, and improves the efficiency of the later maintenance.

[0115] The above embodiments are only for clearly expressing the specific embodiment of the present application, and are not a limitation on the embodiments of the present application. For those skilled in the art, other adjustments or changes of the heating seat assembly 1, the heating body 4, the clamping and fixing mechanism 2, the chromatographic column assembly 3, etc. can be deduced and summarized according to the present application, which are not listed one by one here. Any modification, replacement or improvement made according to the spirit and principle of the present application should be included in the protection scope of the claims of the present application.

Claims

1. A chromatography column device for a glycated hemoglobin analyzer, characterized in that: The application relates to a chromatographic column device. The chromatographic column device comprises a heating seat assembly (1) provided with a first liquid path for liquid inflow and a heating body (4) for heating liquid in the first liquid path; a clamping and fixing mechanism (2) detachably fixed on the heating seat assembly (1) and provided with a chromatographic column cavity (5) axially communicated with a liquid outlet end of the first liquid path; and a chromatographic column assembly (3) provided with a second liquid path on an axis and axially fixed in the chromatographic column cavity (5) and abutting against the liquid outlet end of the first liquid path under the elastic pressing of the action end of the clamping and fixing mechanism (2), so that the third liquid path (6), the second liquid path and the first liquid path are in sealed butt joint communication. The clamping and fixing mechanism (2) comprises a mounting seat (21) provided with a lower cylinder part (211) and an upper cylinder part (212) coaxially connected, a first jack rod through hole (213) axially communicating the inner cavities of the lower cylinder part (211) and the upper cylinder part (212), an opening provided at the bottom end of the lower cylinder part (211) and forming the chromatographic column cavity (5), and at least one guide groove (215) extending around the axis of the upper cylinder part (212) and formed in the cylinder wall of the upper cylinder part (212). A pressure base (22) movably arranged in the upper cylinder part (212) is provided with at least one connecting column (25) radially connected to the side wall of the pressure base (22) and extending through the guide groove (215), and a second jack rod through hole (221) coaxial with the first jack rod through hole (213) is formed in the axis of the pressure base (22). A locking jack rod (23) movably inserted into the second jack rod through hole (221) through an elastic connecting structure, and the third liquid path (6) is formed in the axis of the locking jack rod (23). A knob (24) movably sleeved on the upper cylinder part (212) is fixedly connected with the pressure base (22) through the connecting column (25). When the knob (24) moves downward to the lower limit position under the limiting action of the connecting column (25) and the guide groove (215), the pressure base (22) drives the locking jack rod (23) to elastically abut against the chromatographic column assembly (3) through the first jack rod through hole (213); and when the knob (24) moves upward to the upper limit position, the pressure base (22) drives the locking jack rod (23) to axially separate from the chromatographic column assembly (3). Two guide grooves (215) symmetrically arranged around the axis of the upper cylinder part (212) are formed in the cylinder wall of the upper cylinder part (212), and two connecting columns (25) radially connected to the side wall of the pressure base (22) and extending through the guide groove (215) are formed in the pressure base (22). Vertical anti-skid stripes (242) are uniformly distributed on the side wall of the knob (24). The elastic connecting structure comprises 2. The chromatography column apparatus for a glycated hemoglobin analyzer of claim 1, wherein, ​ 3. The chromatography column apparatus for a glycated hemoglobin analyzer of claim 1, wherein, ​ 4. The chromatography column apparatus of claim 1, wherein the column is a 4.6 mm ID column. 5 ​ A spring cavity (26) is axially arranged in the pressure base (22), and the second top rod through hole (221) penetrates the axis of the spring cavity (26) and communicates with the spring cavity (26); A anti-off portion (231) is radially arranged on the locking top rod (23) and movably arranged in the spring cavity (26), and the outer circumferential dimension of the anti-off portion (231) is greater than the inner diameter of the second top rod through hole (221); A pressure spring (27) is elastically arranged in the spring cavity (26) and movably sleeved on the locking top rod (23), and in a normal state, the lower end of the pressure spring (27) elastically abuts against the anti-off portion (231), and the upper end of the pressure spring (27) elastically abuts against the upper end of the spring cavity (26), so as to elastically push the anti-off portion (231) to the lower end of the spring cavity (26) and drive the first end of the locking top rod (23) to automatically elastically extend out of the lower end of the pressure base (22) through the second top rod through hole (221).

5. The chromatography column device of a glycated hemoglobin analyzer according to claim 1, characterized in that: The guide groove (215) comprises: An arc-shaped groove segment (2151) extends around the axis of the upper cylinder portion (212) and is used for guiding the connection column (25) to drive the pressure base (22) to axially rotate and move to the lower end of the upper cylinder portion (212); A straight groove segment (2152) is located at the upper portion of the arc-shaped groove segment (2151) and communicates with the arc-shaped groove segment (2151), extends axially along the axis of the upper cylinder portion (212), and is used for guiding the connection column (25) to drive the pressure base (22) to axially linearly move to the upper end of the upper cylinder portion (212); A circumferential groove segment (2153) is located at one side of the upper portion of the straight groove segment (2152) and communicates with the straight groove segment (2152), extends along the tangent direction of the outer circumference of the upper cylinder portion (212), and is used for movably clamping the connection column (25) to limit the pressure base (22) at the upper limit position.

6. The chromatography column apparatus of claim 1, wherein the column is a 4.6 mm ID column. The clamping and fixing mechanism (2) further comprises: An assembly opening (28) is formed on the front side of the lower cylinder portion (211) to form an opening at the front portion of the chromatographic column cavity (5), and is used for disassembling and assembling the chromatographic column assembly (3); A protective cover (29) is detachably fixedly connected to the front side of the lower cylinder portion (211) to block the assembly opening (28), and a limiting portion (291) is arranged on the inner side of the protective cover (29) to limit the chromatographic column assembly (3), and a handle (292) is arranged on the outer side of the protective cover (29) to hold the protective cover (29).

7. The chromatography column device of a glycated hemoglobin analyzer according to claim 1, characterized in that: The heating seat assembly (1) comprises: A heat seat (11) is made of a hard heat-conducting material and has a cubic structure, and a liquid channel (111) and a heating cavity (112) located on one side of the liquid channel (111) are arranged on the heat seat (11); A liquid inlet pipe (12) is arranged in the liquid channel (111), and first liquid inlet and outlet end portions (13) and (14) are arranged at the two ends of the liquid inlet pipe (12) and are clamped outside the two ends of the liquid channel (111) and communicate with the inner cavity of the liquid inlet pipe (12); The heating body (4) is fixedly connected to the heat seat (11) and located in the heating cavity (112), and the first liquid outlet end portion (14) is located in the chromatographic column cavity (5) and axially and sealingly clamped to the lower end of the chromatographic column assembly (3).

8. The chromatography column apparatus for a glycated hemoglobin analyzer of claim 7, wherein, The heating seat assembly (1) further comprises: A temperature sensor (15) is fixedly connected to the heat seat (11) and used for detecting the temperature of the heat seat (11).

9. The chromatography column apparatus of claim 7, wherein the column is a 4.6 mm ID x 50 mm long column. 5 The heat seat assembly (1) further comprises: A heat seat cover (16) is a cover structure made of hard heat insulation material and fixedly covers the heat seat (11), and a heat dissipation opening (161) corresponding to the heat generating cavity (112) and a clearance groove (162) exposing the clamping fixing mechanism (2) are formed in the heat seat cover (16).

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