A broken nerve trunk connecting device and its use method

Abstract

The present invention relates to the field of medical equipment, and discloses a fractured nerve trunk connecting device and a method for using the device, comprising a negative pressure device, an identifier, a processor electrically connected to the negative pressure device and the identifier, a receptor electrically connected to the identifier, and a stimulator electrically connected to the processor, wherein the stimulator comprises a stimulating microtube and a stimulating electrode located in the head end of the stimulating microtube, the stimulating electrode is electrically connected to the processor, and the tail end of the stimulating microtube is connected to the negative pressure device; the stimulating microtube and the negative pressure device cooperate to make the stimulating microtube adsorbed on the cell membrane of the fractured nerve trunk, the stimulating electrode in the stimulating microtube is fitted with the neural stem cell membrane, the stimulating microtube seals the stimulating electrode and the cell membrane fitted with the stimulating electrode in the stimulating microtube, and when the effector end of the fractured nerve trunk is stimulated by an electrical signal, the electrical signal can specifically stimulate the cell membrane adsorbed in the stimulating microtube, thereby achieving accurate and safe transmission of the stimulation signal of the receptor end of the fractured nerve trunk to the effector end of the fractured nerve trunk.

Description

Technical Field

[0001] The present invention relates to the field of medical equipment, and in particular to a broken nerve trunk connecting device and a use method thereof. Background Art

[0002] Peripheral nerve injury is mainly caused by trauma, birth injury, bone dysplasia, lead and alcohol poisoning, etc., which cause sensory disorders, motor disorders and nutritional disorders in the areas innervated by the nerves. Peripheral nerves refer to nerves outside the central nervous system (brain and spinal cord). Nerve injury repair is more difficult in clinical practice. The nerve cell repair time after nerve injury is longer. If the patient is in the process of nerve cell repair, the effector controlled by the patient's broken nerve will gradually lose function. Even after the nerve cell self-repairs, it is difficult to restore function. In order to prevent the above situation from happening, external electrical stimulation is usually used to activate the effector to ensure that the function of the effector is not lost, such as the sacral nerve stimulation system and the method for determining the configuration parameters of the sacral nerve stimulation system disclosed in Publication No. CN113491827A. This application relates to a sacral nerve stimulation system and a method for determining the configuration parameters of the sacral nerve stimulation system. The sacral nerve stimulation system includes: a signal acquisition device, an operation control device, a pulse output device and an output feedback device that form a closed-loop control loop. The signal acquisition device is used to obtain a test signal. The operation control device is electrically connected to the signal acquisition device, and is used to store the test signal, and is used to generate the configuration parameters of the sacral nerve stimulation system after analyzing the test signal. The pulse output device is electrically connected to the operation control device, and is used to output the configuration parameters of the sacral nerve stimulation system. The output feedback device is electrically connected to the operation control device, and is used to feed back the feedback signal after the configuration parameters are applied to the operation control device. The type of feedback signal is the same as the type of test signal. The sacral nerve stimulation system automatically controls the configuration parameters of the sacral nerve stimulation system, greatly reducing the energy loss of the system and increasing the service life of the sacral nerve stimulation system.

[0003] Another example is publication number CN101678205B, unidirectional nerve stimulation system, device and method, one embodiment of which relates to a method for delivering unidirectional afferent nerve stimulation therapy. Test nerve stimulation is delivered, and the physiological response to the test nerve stimulation is monitored. If the test nerve stimulation does not induce the desired physiological response, at least one nerve stimulation parameter for the test nerve stimulation is adjusted. If the test nerve stimulation induces the desired physiological response, at least one treatment parameter for unidirectional afferent nerve stimulation is determined using at least one nerve stimulation parameter of the test nerve stimulation that provides the desired physiological response. Unidirectional afferent nerve stimulation is delivered using at least one treatment parameter.

[0004] In the prior art, when the broken nerve trunk connection device and system are working, the stimulation signal of the sensing end of the broken nerve trunk is first identified, and then an algorithm analysis is performed by the processor. Then, the calculated stimulation signal is used to stimulate the effector end of the broken nerve trunk through the same electrical signal as the output of the processor. However, the stimulation current generated by the above-mentioned device will produce electrical stimulation to the nerve trunk and other tissue cells around it, and the current is not stable during stimulation. This indiscriminate stimulation will cause damage to other cells and has low safety. Summary of the invention

[0005] In order to overcome the problem that the broken nerve trunk connecting device in the prior art will produce indiscriminate electrical stimulation to the nerve trunk and other cells around it during operation, resulting in low safety, a broken nerve trunk connecting device and a method of using the same are provided, wherein a stimulation microtube and a negative pressure device cooperate to allow the stimulation microtube to be adsorbed on the cell membrane of the broken nerve trunk, after adsorption, the stimulation electrode in the stimulation microtube is bonded to the neural stem cell membrane, the stimulation microtube seals the stimulation electrode and the cell membrane bonded to the stimulation electrode in the stimulation microtube, and when the effector end of the broken nerve trunk is stimulated by an electrical signal, the electrical signal can specifically stimulate the cell membrane adsorbed in the stimulation microtube, thereby achieving accurate and safe transmission of the stimulation signal from the sensing end of the broken nerve trunk to the effector end of the broken nerve trunk.

[0006] The specific technical scheme of the present invention is:

[0007] A device for connecting a broken nerve trunk comprises a negative pressure device, an identifier, a processor electrically connected to the negative pressure device and the identifier, a receptor electrically connected to the identifier, and a stimulator electrically connected to the processor, wherein the stimulator comprises a stimulating electrode and a stimulating electrode located in the head end of a stimulating microtube, the stimulating electrode is electrically connected to the processor, and the tail end of the stimulating microtube is connected to the negative pressure device.

[0008] The present application provides a device for connecting a broken nerve trunk. By setting a negative pressure device, a stimulating microtube and a stimulating electrode, the technical effect of accurately and safely transmitting the stimulation signal of the sensing end of the broken nerve trunk to the effector end of the broken nerve trunk is achieved; the stimulating microtube can be adsorbed on the cell membrane of the nerve cell through the negative pressure generated by the negative pressure device, and the stimulating electrode is adsorbed on the cell membrane in the stimulating microtube and fits together. At this time, the cell membrane in the stimulating microtube and the outside world form an electrical shielding space. When the cell membrane in the shielding space is stimulated by the electrical signal, it can be free from external interference, the electrical signal distortion is low, and the restored degree of the transmitted stimulation electrical signal is also higher and more accurate; in addition, the stimulating microtube is fixed on the neural stem cell by negative pressure adsorption, which will not damage the neural stem cell and has high safety.

[0009] Preferably, the stimulation electrode comprises a positive stimulation ear, a negative stimulation ear and a stimulation electrode base coated on the outside of the positive stimulation ear and the negative stimulation ear, the positive stimulation ear and the negative stimulation ear are located on both sides of the central axis of the stimulation electrode base, the tail end of the stimulation electrode base is provided with a stimulation electrode slot located in the tail end of the stimulation microtube, and the stimulation electrode slot is provided with a stimulation electrode sheet electrically connecting the positive stimulation ear and the negative stimulation ear; the positive stimulation ear and the negative stimulation ear of the present application form a closed electrical circuit after being attached to the cell membrane, and can transmit electrical signals to the cell membrane when power is turned on, thereby achieving electrical stimulation of the cell membrane.

[0010] Preferably, the distance from the end face of the positive stimulation pole ear and the end face of the negative stimulation pole ear to the end face of the stimulation microtube is 1 to 10 μm; by limiting the distance from the end face of the stimulation pole ear to the end face of the stimulation microtube, it is possible to prevent the stimulation electrode from being too far away from the cell, and when the nerve cells are adsorbed, the nerve cells are damaged before they are attached to the stimulation electrode and the sensing electrode.

[0011] Preferably, the materials of the positive stimulation electrode and the negative stimulation electrode are selected from any one or more of graphene, carbon nanotubes, biocompatible conductive polymers, and biocompatible conductive metals; when the device is used on living cells, it is necessary to use materials with good biocompatibility to prepare the electrodes, which can improve the safety of the broken nerve trunk connection device.

[0012] Preferably, the inner diameter of the head end of the stimulation microtube is 0.5-100 μm.

[0013] Preferably, an electrical conductor is provided at the tail end of the stimulation microtube, and the electrical conductor includes a microtube air plug, a microtube electric plug, a box air plug, a box electric plug, a conductive wire connecting the microtube electric plug and the box electric plug, and an electrical conductor matrix coated on the conductive wire, an airway connecting the microtube air plug and the box air plug is provided in the electrical conductor matrix, the microtube air plug is connected to the stimulation microtube, the box air plug is connected to the negative pressure device, the microtube electric plug is connected to the stimulation electrode, and the box electric plug connected to the stimulation microtube is electrically connected to the processor.

[0014] The electrical wire of the present application electrically connects the receptor and the identifier to the processor and communicates with the negative pressure device respectively. The receptor is composed of a sensing microtube and a sensing electrode inside the sensing microtube, and the stimulator is composed of a stimulating microtube and a stimulating electrode. Both need to be electrically connected and gas-connected at the same time when in use. Therefore, the electrical wire connecting the receptor and the identifier also needs to have the function of transmitting electrical signals and transmitting gas at the same time. The electrical wire matrix of the present application is provided with a conductive wire for transmitting electrical signals and an airway for transmitting gas, which can achieve the effect of simultaneously transmitting electrical signals and gas to the receptor and the identifier.

[0015] Preferably, the sensing electrode is a patch clamp.

[0016] Preferably, the patch clamp is selected from any one of a resistive patch clamp and a capacitive patch clamp.

[0017] A method for using a broken nerve trunk connecting device comprises the following steps:

[0018] (1) turning on the identifier and the processor, connecting the receptor to the sensing end of the severed nerve trunk, continuously stimulating the sensing end of the severed nerve trunk, and stopping stimulating the sensing end of the severed nerve trunk after the waveform of the identified potential signal becomes stable;

[0019] (2) Turn on the negative pressure device and attach the stimulator to the effector end of the severed nerve trunk;

[0020] (3) The sensory end of the severed nerve trunk is stimulated, and the effector end of the severed nerve trunk produces a corresponding response to the stimulation, thus completing the connection of the severed nerve trunk.

[0021] The present application also provides a method for using a broken nerve trunk connecting device, which can accurately and safely transmit the stimulation signal of the sensing end of the broken nerve trunk to the effector end of the broken nerve trunk, thereby completing the connection of the broken nerve trunk.

[0022] Compared with the prior art, this application has the following technical effects:

[0023] (1) The fractured nerve trunk connection device provided in the present application achieves the technical effect of accurately and safely transmitting the stimulation signal of the sensory end of the fractured nerve trunk to the effector end of the fractured nerve trunk by setting a negative pressure device, a stimulation microtube and a stimulation electrode;

[0024] (2) The cell membrane in the stimulating microtubule forms an electrical shielding space with the outside world. When the cell membrane in the shielding space is stimulated by electrical signals, it is not disturbed by the outside world, the electrical signal distortion is low, and the transmitted stimulation electrical signal restoration degree is higher and more accurate;

[0025] (3) The stimulating microtubules are fixed on the neural stem cells by negative pressure adsorption, which will not damage the neural stem cells and is highly safe. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the present invention.

[0027] Figure 2 is a cross-sectional view of the stimulator of the present invention.

[0028] Figure 3 It is a cross-sectional view of an electrical conductor of the present invention.

[0029] Figure 4It is a waveform diagram of the potential signal when the present invention stimulates the severed nerve trunk of a frog.

[0030] In the figure, there are negative pressure device 1, identifier 2, processor 3, receptor 4, stimulator 5, stimulation microtube 6, stimulation electrode 7, positive stimulation pole ear 701, negative stimulation pole ear 702, stimulation electrode base 703, stimulation electrode slot 704, stimulation electrode sheet 705, electrical wire 8, microtube air plug 801, microtube electric plug 802, box air plug 803, box electric plug 804, conductive wire 805, electrical wire base 806 and airway 807. DETAILED DESCRIPTION

[0031] The present invention will be further described below in conjunction with the embodiments.

[0032] Embodiment 1:

[0033] like Figure 1 As shown, a device for connecting a broken nerve trunk includes a negative pressure device 1, an identifier 2, a processor 3 electrically connected to the negative pressure device and the identifier, a receptor 4 electrically connected to the identifier, and a stimulator 5 electrically connected to the processor, the receptor is a HEKA EPC-9 patch clamp, and the software installed in the processor is PULSE / PULSEFIT.

[0034] like Figure 2 As shown, the stimulator includes a stimulation microtube 6 and a stimulation electrode 7 located in the head end of the stimulation microtube, the stimulation microtube is 2 cm long, the inner diameter of the head end of the stimulation microtube is 50 μm, the stimulation electrode is electrically connected to the processor, the tail end of the stimulation microtube is connected to the negative pressure device, the stimulation electrode includes a positive stimulation pole ear 701, a negative stimulation pole ear 702 and a stimulation electrode base 703 coated outside the positive stimulation pole ear and the negative stimulation pole ear, the positive stimulation pole ear and the negative stimulation pole ear are located on both sides of the central axis of the stimulation electrode base, the tail end of the stimulation electrode base is provided with a stimulation electrode slot 704 located in the tail end of the stimulation microtube, the stimulation electrode slot is provided with a stimulation pole ear and a stimulation electrode sheet 705 connected to the stimulation pole ear, the distance from the head end end face of the positive stimulation pole ear and the head end end face of the negative stimulation pole ear to the head end end face of the stimulation microtube is 4 μm.

[0035] like Figure 3 As shown, an electrical conductor 8 is provided at the tail end of the stimulation microtube, and the electrical conductor is 10 cm long. The electrical conductor includes a microtube air plug 801, a microtube electric plug 802, a box air plug 803, a box electric plug 804, a conductive wire 805 connecting the microtube electric plug and the box electric plug, and an electrical conductor matrix 806 coated on the conductive wire. An airway 807 connecting the microtube air plug and the box air plug is provided in the electrical conductor matrix. The microtube air plug is connected to the stimulation microtube, the box air plug is connected to the negative pressure device, the microtube electric plug is connected to the stimulation electrode, and the box electric plug connected to the stimulation microtube is electrically connected to the processor.

[0036] A method for using a broken nerve trunk connecting device comprises the following steps:

[0037] (1) turning on the identifier and the processor, connecting the receptor to the sensing end of the severed nerve trunk, continuously stimulating the sensing end of the severed nerve trunk, and stopping stimulating the sensing end of the severed nerve trunk after the waveform of the identified potential signal becomes stable;

[0038] (2) Turn on the negative pressure device and attach the stimulator to the effector end of the severed nerve trunk;

[0039] (3) The sensory end of the severed nerve trunk is stimulated, and the effector end of the severed nerve trunk produces a corresponding response to the stimulation, thus completing the connection of the severed nerve trunk.

[0040] Embodiment 2:

[0041] Compared with Example 1, the material of the stimulation electrode is carbon nanotubes, the thickness of the carbon nanotubes is 100 nm, and the other conditions are the same as those in Example 1.

[0042] Embodiment 3:

[0043] Compared with Example 1, the stimulation electrode is a PEDOT:PSS polylactic acid electrode, and the PEDOT:PSS is coated on a polylactic acid substrate. The other conditions are the same as those in Example 1.

[0044] Embodiment 4:

[0045] Compared with Example 1, the material of the stimulation electrode is platinum-iridium alloy, the electrode thickness is 50 nm, and the other conditions are the same as those in Example 1.

[0046] Embodiment 5:

[0047] The broken nerve trunk connecting device and practical method of Example 1 are used to connect the broken nerve trunks of frogs.

[0048] Comparative Example 1:

[0049] The broken nerve trunks of the frog in Example 5 were connected using a broken nerve trunk connecting device in the prior art.

[0050] Test example

[0051] A patch clamp potential identifier is set at the effector end of the frog's broken nerve trunk in Example 5 and Comparative Example 1, and different stimulations are applied to the sensing end of the frog's broken nerve trunk, and the potential signal waveform generated by the sensing end of the broken nerve trunk and the potential signal waveform generated by the effector end of the broken nerve trunk are recorded.

[0052] like Figure 4As shown, the potential signal generated by the sensing end of the broken nerve trunk in Example 5 has a high degree of consistency and high fidelity with the potential signal generated by the effector end of the broken nerve trunk, while the potential signal generated by the sensing end of the broken nerve trunk in Comparative Example 1 has a serious electrical signal distortion and low fidelity compared with the potential signal generated by the effector end of the broken nerve trunk. The experimental results show that the broken nerve trunk connecting device and use method provided in the present application can achieve the technical effect of accurately and safely transmitting the stimulation signal of the sensing end of the broken nerve trunk to the effector end of the broken nerve trunk compared with the prior art.

[0053] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A device for connecting a broken nerve trunk, characterized in that: The invention comprises a negative pressure device (1), an identifier (2), a processor (3) electrically connected to the negative pressure device and the identifier, a receptor (4) electrically connected to the identifier, and a stimulator (5) electrically connected to the processor, wherein the stimulator comprises a stimulating microtube (6) and a stimulating electrode (7) located in the head end of the stimulating microtube, wherein the stimulating electrode is electrically connected to the processor, and the tail end of the stimulating microtube is connected to the negative pressure device. The tail end of the stimulating microtube is provided with an electrical wire (8), wherein the electrical wire comprises a microtube air plug (801), a microtube electrical plug ( 802), a box air plug (803), a box electric plug (804), a conductive wire (805) connecting the microtube electric plug and the box electric plug, and an electrical wire matrix (806) coated on the conductive wire, wherein an airway (807) connecting the microtube air plug and the box air plug is provided in the electrical wire matrix, the microtube air plug is connected to the stimulation microtube, the box air plug is connected to the negative pressure device, the microtube electric plug is connected to the stimulation electrode, and the box electric plug connected to the stimulation microtube is electrically connected to the processor.

2. A broken nerve trunk connection device as claimed in claim 1, characterized in that: The stimulation electrode comprises a positive stimulation pole ear (701), a negative stimulation pole ear (702), and a stimulation electrode base (703) coated outside the positive stimulation pole ear and the negative stimulation pole ear, wherein the positive stimulation pole ear and the negative stimulation pole ear are located on both sides of the central axis of the stimulation electrode base, and the tail end of the stimulation electrode base is provided with a stimulation electrode slot (704) located in the tail end of the stimulation microtube, and the stimulation electrode slot is provided with a stimulation electrode sheet (705) electrically connected to the positive stimulation pole ear and the negative stimulation pole ear.

3. A broken nerve trunk connection device as claimed in claim 2, characterized in that: The distance between the end surface of the positive stimulation pole ear and the end surface of the negative stimulation pole ear and the end surface of the stimulation microtube is 1-10 μm.

4. A broken nerve trunk connection device as claimed in claim 2, characterized in that: The materials of the positive stimulation tabs and the negative stimulation tabs are selected from any one or more of graphene, carbon nanotubes, biocompatible conductive polymers, and biocompatible conductive metals.

5. A broken nerve trunk connecting device as claimed in claim 2, characterized in that: The inner diameter of the head end of the stimulation microtube is 5-100 μm.

6. A broken nerve trunk connecting device as claimed in claim 1, characterized in that: The sensor is a patch clamp.

7. A broken nerve trunk connecting device as claimed in claim 6, characterized in that: The patch clamp is selected from any one of a resistance patch clamp and a capacitance patch clamp.

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