An internal staining device and staining method for fish specimens

Abstract

The present invention relates to an internal staining device and a staining method for fish specimens, belonging to the field of specimen production. The device includes an outer box body and an inner box body. The outer box body is a box-shaped body. The inner box body is composed of a first partition board, a second partition board, two side walls of the outer box body, and a bottom plate. The first partition board and the second partition board are fixed inside the outer box body. The gaps between the first partition board, the second partition board and the outer box body form a dye solution pool. The bottom plate of the inner box body is a specimen static platform made of a rubber material, which is penetrated by metal capillary tubes up and down. The metal capillary tubes can move up and down and are communicated with an external staining pool. When using the present invention to stain a fish body, the position, quantity and depth of the metal capillary tubes inserted into the fish body to be stained are adjusted according to the required staining area, and the fish body is stained by means of siphon action, concentration diffusion and capillary action, with uniform staining and accurate position.

Description

Technical Field

[0001] The present invention belongs to the field of specimen staining, and particularly relates to an internal staining device and a staining method for fish specimens. Background Art

[0002] For ordinary immersion specimen staining, it only needs to immerse the fish in a specific staining solution. However, for specimens that require unilateral staining, internal staining, or have too thick fish skin or too dense muscle tissue, good staining effects cannot be obtained through conventional staining methods, and phenomena such as uneven staining or even failure to stain will occur. Or when making specimens, only one side of the fish body skeleton needs to be stained, while the other side needs to maintain the original body color of the fish. Currently, the soaking method is usually used for staining, but it is very difficult to perform unilateral staining by soaking, and it is easy for the staining solution to spread to the other side, resulting in staining failure. Summary of the Invention

[0003] The present invention provides a novel internal staining device and a staining method for fish specimens, which can effectively solve the above technical problems.

[0004] The present invention is realized through the following technical solutions:

[0005] An internal staining device for fish specimens, the device includes an outer box body and an inner box body. The outer box body is a box-shaped body. The inner box body is composed of a first partition board, a second partition board, two side walls of the outer box body, and a bottom board. The first partition board and the second partition board are fixed inside the outer box body. The gaps between the first partition board and the second partition board and the outer box body form a dye solution pool. There is a 2 - 3 cm gap between the bottom of the inner box body and the bottom of the outer box body. The dye solution pool formed by the outside of the inner box body and the inside of the outer box body is interconnected. The bottom board of the inner box body consists of a specimen static placement table. The specimen static placement table is a rubber material area. Metal capillary tubes penetrate up and down in the rubber material area. The number of metal capillary tubes can be increased or decreased according to the shape of the specimen to be stained, and the capillary tube area can be set corresponding to the specimen shape, and the metal capillary tubes can move up and down. The metal capillary tubes are connected to an external staining pool.

[0006] The usage method of the above device: Insert metal capillary tubes on the specimen static placement table according to the size, shape of the fish specimen and the position of the required staining area. Place one side of the fish specimen to be stained on the metal capillary tubes, and press down to make the metal capillary tubes penetrate into the fish body tissue. At the same time, adjust the insertion position, number, and depth of the metal capillary tubes according to the required staining area. Pour the specimen staining solution into the dye solution pool, and the liquid level of the staining solution is higher than the bottom of the inner box body. Under the capillary action and siphon action, the staining solution enters the internal structure of the fish through the metal capillary tubes, and uniformly and thoroughly stains one side below the fish body according to the concentration diffusion and gravity action, without staining the outside of the fish body, especially the upper side of the fish body, which is not affected by the staining.

[0007] Furthermore, when performing unilateral staining on a fish body, place the fish body to be kept intact facing upwards and cover it with a wet wipe to prevent water evaporation. Place the other side to be stained facing downwards and insert a metal capillary into the muscle. Adjust the depth of the metal capillary inserted into the fish body according to the staining depth.

[0008] Advantages of the present invention compared with the prior art:

[0009] (1) The bottom of the specimen resting table of the present invention is a partial rubber area, which has some tension itself. There will be no obvious damage after inserting the capillary, and it can play a sealing role to prevent the staining solution from seeping out from positions other than the capillary.

[0010] (2) The rubber area at the bottom of the staining pool of the present invention is a detachable design. The metal capillary can be assembled according to the size and shape of the specific fish specimen and the position of the required staining area. It can be easily replaced after being damaged due to excessive use.

[0011] (3) The present invention stains the interior of the fish using principles such as siphon action, capillary action, and concentration diffusion caused by water potential gradient. Adjust the position, quantity, and depth of the capillary insertion according to the required staining area, with uniform staining and precise position. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 : Schematic structural diagram of the device of the present invention;

[0013] In the figure: 1. Outer box body, 2. First partition board, 3. Second partition board, 4. Bottom board, 5. Dyeing liquid pool, 6. Specimen resting table, 7. Metal capillary. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0014] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0015] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0016] A device for internal staining of fish specimens, as Figure 1As shown in the figure, the device includes an outer box body 1 and an inner box body. The outer box body 1 is a box-shaped body. The inner box body is composed of a first partition 2, a second partition 3, two side walls of the outer box body 1, and a bottom plate 4. The first partition 2 and the second partition are fixed inside the outer box body 1. The gaps between the first partition 2 and the second partition 3 and the outer box body 1 form a dye solution pool 5. There is a 3-cm gap between the bottom of the inner box body and the bottom of the outer box body 1. The dye solution pool 5 formed by the outside of the inner box body and the inside of the outer box body 1 is interconnected. The bottom plate 4 of the inner box body consists of a specimen resting table 6. The specimen resting table 6 is a rubber material area. Metal capillary tubes 7 penetrate up and down in the rubber material area. The metal capillary tubes 7 can increase or decrease the number of metal capillary tubes according to the shape of the specimen to be stained and set the capillary tube area into the shape of the specimen. Moreover, the metal capillary tubes 7 can move up and down. The metal capillary tubes 7 are connected to an external staining pool.

[0017] Insert the metal capillary tubes 7 into the rubber material area. The specific positions and quantities can be arranged according to the size and shape of the specific fish specimen and the position of the area to be stained. Place one side of the fish specimen to be stained on the capillary tubes and slightly press down to make the capillary tubes pierce into the fish body tissue. Adjust the insertion position, quantity, and depth of the capillary tubes according to the area to be stained. Then pour the fish specimen staining solution into the dye solution pool 5. Under the combined action of capillary action, siphon action, gravity, and concentration diffusion caused by the water potential gradient, the internal structure of the fish body can be stained more evenly and thoroughly.

[0018] When performing unilateral staining: Place the fish on the staining device and insert the lower half into the capillary tubes. The orifice (i.e., the top) of the capillary tubes is close to but does not exceed the mid-axis of the fish body, that is, it reaches near the plane of the vertebral column, neural spine, and haemal spine. Cover the upper surface of the fish with a wet tissue to keep the moisture in the upper half. When pouring the bone staining solution into the dye solution pool 5, adjust the height difference between the liquid level of the staining solution and the orifice of the capillary tubes according to the muscle thickness of the fish body. The thicker the muscle layer, the greater the liquid level difference. The hydraulic pressure formed by the generated liquid level difference causes the staining solution to enter the fish body through the capillary tubes upward and diffuse out. Since the lower surface of the fish body is exposed to the air, the evaporation of the preservation solution and moisture will form an internal and outer water potential gradient, which will drive the internal liquid of the fish body to diffuse towards the lower surface of the fish body. Coupled with the action of gravity, the staining solution will diffuse out from the orifice of the capillary tubes and penetrate downward to stain the bones in the muscle of the lower half of the fish body. And it will not stain the outside of the fish body, especially the upper side of the fish body, which is not affected by the staining.

[0019] Use the above staining device to perform unilateral bone staining on Sebastes schlegelii. First, remove the fish skin on the side to be stained. Then, place the side to be stained into the device according to the above operation. Pour alizarin red staining solution 3 cm higher than the bottom surface of the inner box body into the staining pool. After staining, dissect the stained side of the fish body to expose the bones. It is found that the bones of the stained side of the fish body are stained evenly, and the pigment does not extend upward to the bones on the other side and the fish body surface.

[0020] The present invention has been described by way of example in conjunction with the accompanying drawings. Obviously, the specific implementation of the present invention is not limited by the above methods. As long as such non-substantial improvements are made by adopting the method concept and technical solution of the present invention, or the concept and technical solution of the present invention are directly applied to other occasions without improvement, they are all within the protection scope of the present invention.

Claims

1. A device for internal staining of fish specimens, characterized in that, the device comprises an outer box body (1) and an inner box body. The outer box body (1) is a box-shaped body. The inner box body is composed of a first partition (2), a second partition (3), two side walls of the outer box body (1) and a bottom plate (4). The first partition (2) and the second partition (3) are fixed inside the outer box body (1). The gaps between the first partition (2) and the second partition (3) and the outer box body (1) form a dye solution pool (5). There is a gap of 2 - 3 cm between the bottom of the inner box body and the bottom of the outer box body (1). The dye solution pool (5) formed by the outside of the inner box body and the inside of the outer box body (1) is interconnected. The bottom plate (4) of the inner box body has a specimen resting table (6). The specimen resting table (6) is made of rubber. Metal capillary tubes (7) penetrate vertically through the rubber material area. The rubber material area plays a sealing role to prevent the staining solution from seeping out from positions other than the capillary tubes. The metal capillary tubes (7) can increase or decrease the number of metal capillary tubes according to the shape of the specimen to be stained and set the capillary tube area corresponding to the specimen shape, and the metal capillary tubes (7) can move up and down. The metal capillary tubes (7) are connected to the external dye solution pool.

2. The method for using the device according to claim 1, characterized in that, insert metal capillary tubes (7) on the specimen resting table (6) according to the size, shape of the fish specimen and the position of the area to be stained. Place one side of the fish specimen to be stained on the metal capillary tubes (7) and press down to make the metal capillary tubes (7) penetrate into the fish body tissue. At the same time, adjust the insertion position, number and depth of the metal capillary tubes (7) according to the area to be stained. Pour the specimen staining solution into the dye solution pool (5). The liquid level of the staining solution is higher than the bottom of the inner box body. Under the action of capillary action, siphon action, concentration diffusion caused by water potential gradient and gravity, the staining solution enters the internal structure of the fish body through the metal capillary tubes (7) to uniformly and thoroughly stain one side of the fish body without staining the outside of the fish body.

3. According to the method of claim 2, characterized in that, when performing unilateral staining on the fish body, place the fish body to be kept intact upwards and cover it with a wet tissue to prevent water evaporation. Place the other side to be stained downwards and insert the metal capillary tubes (7) into the muscle. Adjust the depth of the metal capillary tubes (7) inserted into the fish body according to the staining depth.

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