A natural light water environment introduction module based on AAO

Abstract

The present invention discloses an AAO-based natural light water environment introduction module, which belongs to the fields of optical fiber sensing technology and zebrafish biotechnology, including a natural light receiving end, an optical fiber introduction, an AAO-based natural light output end and a zebrafish constant temperature culture water tank, wherein the natural light receiving end is connected to the optical fiber introduction device, and natural light is introduced below the water surface A of the zebrafish constant temperature culture water tank, and the AAO-based is a porous alumina template with an aperture of 100-300nm, which is coupled to the optical fiber output end after preparation to achieve the position and light intensity adjustability of natural light. The present invention enables zebrafish laboratory cultivation to simulate the real light environment of the outside world through the natural light optical fiber introduction mode, and at the same time, the natural light is prepared by AAO-based materials so that the natural light has adjustability, thereby serving various biomedicine and environmental toxicology experiments based on the zebrafish model.

Description

Technical Field

[0001] The invention relates to the technical fields of zebrafish biotechnology and optical fiber light guide, and in particular to an AAO-based natural light water environment introduction module. Background Art

[0002] With the rapid development of my country's economy and the rapid expansion of industry and agriculture, water environmental problems are becoming increasingly serious. Zebrafish have been commonly used in water quality environmental testing in recent years because of their large egg-laying capacity, transparent embryos, short reproductive cycle, and high sensitivity to toxic and harmful substances. At the same time, zebrafish and humans have a genetic similarity of up to 87%, and have become the standard model organism for biomedicine and environmental toxicology in the world. They are widely used in routine toxicity tests, molecular and cellular ecotoxicology research, and biological induction effect detection, and are important biological models for ecotoxicological evaluation.

[0003] In existing zebrafish laboratory cultivation, artificial light sources such as LED lamps are often used to create a lighting environment. At the same time, the lighting conditions are relatively fixed and cannot be adjusted according to the experimental needs and the growth and development needs of zebrafish. However, more and more studies have shown that compared with natural light that is full-band and changes dynamically over time, LED lights have the disadvantages of being concentrated in short waves and having fixed wavelengths. Long-term exposure will significantly interfere with the growth and development of zebrafish larvae and cause oxidative stress and inflammatory responses. The long-term fixed lighting environment is also far from reality, ignoring the seasonal differences in the light environment itself and dynamic changes such as urban light pollution, which greatly weakens the actual reference value of ecological risk assessment based on the zebrafish model. Therefore, how to establish a light environment that is more in line with reality and reduces artificial interference in zebrafish experiments, while increasing the adjustability of light, is a key technical problem that needs to be solved urgently. Summary of the invention

[0004] The present invention proposes an AAO-based natural light water environment introduction module which collects external natural light in real time through a natural light receiving end, transmits it into a zebrafish culture tank or even below the water surface via an optical fiber, and adjusts the natural light position and lighting parameters through an AAO-based coupled optical fiber output end.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The invention discloses an AAO-based natural light water environment introduction module, comprising a natural light receiving end, an optical fiber introduction device and a zebrafish constant temperature culture water tank; the natural light receiving end comprises an upper opening focusing lens and a small solar panel; the small solar panel is arranged at the center of the bottom of the upper opening focusing lens; the small solar panel is connected to the zebrafish constant temperature culture water tank through the optical fiber introduction device, one end of the optical fiber introduction device is arranged under the water surface of the zebrafish constant temperature culture water tank, so as to introduce natural light into the zebrafish constant temperature culture water tank.

[0007] Preferably, the natural light receiving end also includes a rotating tracking mechanism and a light sensor; the rotating tracking mechanism is arranged at the bottom of the focusing lens, the light sensor is arranged at the center of the focusing lens and above the rotating tracking mechanism, the rotating tracking mechanism and the light sensor are connected, the small solar panel is arranged between the rotating tracking mechanism and the light sensor, and the light sensor senses the sun's position in real time and drives the rotating tracking mechanism to directionally track natural light.

[0008] Preferably, the optical fiber introduction device comprises a light-guiding optical fiber, a converter and an AAO-based optical fiber output end; the input end of the light-guiding optical fiber is connected to a focusing lens, and the output end of the light-guiding optical fiber extends to the inside of a zebrafish constant temperature culture tank and is connected to the AAO-based optical fiber output end through the converter and the optical fiber end.

[0009] Preferably, the AAO-based optical fiber output end comprises a porous alumina template, and the porous alumina template is coupled to the end of the optical fiber.

[0010] Preferably, the pore size and thickness of the porous alumina template can be replaced to adjust the intensity of the introduced natural light.

[0011] Preferably, the pore size of the porous alumina template is 500-5000 nm.

[0012] Preferably, the optical fiber introduction device also includes an optical fiber control slide rail, which is connected to the light-guiding optical fiber in the zebrafish constant temperature culture tank; an intelligent controller is provided on the top of the zebrafish constant temperature culture tank, and the intelligent controller controls the movement of the light-guiding optical fiber in the optical fiber control slide rail to change the horizontal and vertical position of the AAO-based natural light output end in the water, as well as the underwater depth.

[0013] Preferably, an electronic thermometer is provided on the inner wall of the zebrafish constant temperature culture tank, and the electronic thermometer is arranged below the water surface.

[0014] Preferably, the zebrafish constant temperature culture water tank comprises an inner shell and an outer shell, a heating tube and a heat preservation material are arranged between the inner shell and the outer shell; one end of the heating tube is connected to a small solar panel for receiving energy transmission from the small solar panel, and the other end of the heating tube is connected to an electronic thermometer; a constant temperature control circuit board is arranged on one side of the zebrafish constant temperature culture water tank, and the heating tube is connected to the power control of the constant temperature control circuit board and the intelligent controller.

[0015] Preferably, at least two underwater illuminometers are provided on the side walls of the zebrafish constant temperature culture tank; the constant temperature control circuit board is connected to the power supply of the underwater illuminometer, the underwater illuminometer is connected to a remote computer via wireless, and the underwater illuminometer is used to detect underwater light at different depths and transmit the illuminance data to the computer in real time.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The present invention introduces natural light into the zebrafish breeding environment with the help of optical fibers, so that the laboratory lighting conditions change dynamically with the external environment, solving the technical problem that most of the current zebrafish breeding uses artificial light sources and the lighting is fixed, which is not conducive to the normal growth and development of fry. It eliminates the negative interference of artificial lighting for the current biomedical and environmental toxicology experiments based on the zebrafish model.

[0018] 2. The present invention realizes the regulation of multiple levels of illumination through the preparation of AAO-based materials and optical fiber coupling. The illumination output by the optical fiber passes through AAO-based materials of different sizes, thereby introducing natural light of different light intensities to meet the illumination requirements of zebrafish growth and development at a specific stage; the position of the output natural light is jointly controlled by the optical fiber control slide, converter and intelligent controller, thereby realizing the irradiation of point light sources of natural light at different horizontal and vertical positions to meet the requirements of zebrafish behavioral tests (such as light avoidance, sinking experiments, etc.); it can realize the introduction of sunlight below the water surface, and the illumination depth can be adjusted at the same time.

[0019] 3. The present invention can be directly used in current urban light pollution research, especially nighttime light pollution. Nowadays, light pollution has become a new environmental problem that has received much attention, but current related laboratory research is still relatively rough, and it is difficult to accurately simulate the illumination and wavelength of nighttime dim light. Often, only a specific wavelength of light (usually blue light or white light) is irradiated for a fixed period of time at night. If the light receiving end of the device is turned on normally at night, the nighttime city light can also be collected and transmitted to the zebrafish breeding tank in real time for exposure, providing a convenient way to simulate light pollution.

[0020] 4. Furthermore, through the synergy of the light sensor and the rotation tracking mechanical device in the natural light receiving end, the present invention can dynamically change the lighting direction of the device in real time. At the same time, light collection consumes zero energy, and the full absorption of solar energy can also be supplied to the zebrafish culture tank for constant temperature control. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a structural schematic diagram of an AAO-based natural light water environment introduction module proposed by the present invention;

[0022] Figure 2 This is a schematic structural diagram of a natural light receiving end in an AAO-based natural light water environment introduction module proposed by the present invention;

[0023] Figure 3 This is a schematic structural diagram of an AAO-based optical fiber output end in an AAO-based natural light water environment introduction module proposed by the present invention;

[0024] Figure 4 This is a schematic diagram of the internal structure of a zebrafish constant temperature culture tank in an AAO-based natural light water environment introduction module proposed by the present invention.

[0025] The numbers in the figure are as follows:

[0026] 1. Rotation tracking mechanism; 2. Light sensor; 3. Focusing lens; 4. Small solar panel; 5. Natural light receiving end; 6. Light-guiding optical fiber; 7. AAO-based optical fiber output end; 8. Porous alumina template; 9. Optical fiber end; 10. Optical fiber control slide rail; 11. Converter; 12. Intelligent controller; 13. Zebrafish constant temperature culture tank; 14. Electronic thermometer; 15. Constant temperature control circuit board; 16. Underwater illuminance meter; 17. Heat pipe. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0028] Embodiment 1

[0029] like Figures 1 to 3 As shown, an AAO-based natural light water environment introduction module provided by the present invention includes a natural light receiving end 5, a light-guiding optical fiber 6, an AAO-based optical fiber output end 7 and a zebrafish constant temperature culture water tank 13; the natural light receiving end 5 includes a rotation tracking mechanical device 1, a light sensor 2, a focusing lens 3 and a small solar panel 4; the AAO-based optical fiber output end 7 includes a porous aluminum oxide 8, an optical fiber end 9, an optical fiber regulating slide rail 10 and a converter 11; the zebrafish constant temperature culture water tank 13 includes an electronic thermometer 14, a constant temperature control circuit board 15, an intelligent controller 12, an underwater illuminance meter 16 and a heating tube 17;

[0030] The rotating tracking mechanism 1 is located at the bottom of the natural light receiving end 5, the light sensor 2 is located above the rotating tracking mechanism 1 and in the center of the natural light receiving end 5, the focusing lens 3 is arranged around the light sensor, and the small solar panel 4 is sandwiched between the rotating tracking mechanism 1 and the light sensor 2; the light sensor 2 senses the direction of natural light and sends a signal to the rotating tracking mechanism 1, and the rotating tracking mechanism 1 rotates at an angle to realize automatic tracking and active collection of natural light, while the light sensor 2 is controlled by the intelligent controller 12; the focusing lens 3 can be composed of a plurality of small focusing lenses, which are Fresnel lenses or quartz lenses, depending on the specific implementation situation, and the focusing lens 3 focuses natural light and couples it into the light-guiding optical fiber 6; the small solar panel 4 has the effect of absorbing and storing solar energy, is connected to the heating tube 17 circuit, and is controlled by the intelligent controller 12; the natural light receiving end 5 can be installed on the roof or the side wall of the house, and the installation should be careful to avoid interference from surrounding reflected light;

[0031] The light-guiding optical fiber 6 described in the present invention adopts the principle of total reflection to conduct light with zero energy consumption. Each light-guiding optical fiber 6 corresponds to a focusing lens 3, and no energy conversion occurs in the middle. The natural light introduced by the light-guiding optical fiber 6 does not change the spectral form of the solar tube, and does not have the common problems of missing spectral lines or serious imbalance of different wavelength ratios of artificial light sources, thereby reducing the potential harm of lighting to the growth and development of organisms. The optical fiber end 9 of the light-guiding optical fiber 6 is connected to the AAO-based optical fiber output end 7.

[0032] The AAO-based optical fiber output end 7 uses a porous alumina template 8, and the porous alumina template 8 is prepared by a secondary oxidation method, and the preparation method is as follows:

[0033] S1: A high-purity aluminum sheet is used as a substrate and subjected to degreasing and surface decontamination treatment.

[0034] S2: Add 400 mL of polishing liquid into a 500 mL beaker, in which the volume ratio of perchloric acid to ethanol is 1:4, and add magnetic beads to make the mixture more uniform.

[0035] S3: Use high-purity aluminum sheet as anode and graphite as cathode for electropolishing. The water bath reaction temperature is 7°C, the voltage is 20V, and the reaction time is 3min.

[0036] S4: Anodizing the polished aluminum sheet using oxalic acid solution as an electrolyte, with a voltage of 60 V and an oxidation time of 4 hours.

[0037] S5: Clean the alumina template obtained after the first anodization, and remove the generated oxide film in a mixed solution of phosphoric acid and chromic acid.

[0038] S6: Perform secondary oxidation again, and place the aluminum sheet back into the oxalic acid electrochemical reaction solution to obtain a highly ordered nanoscale porous positive alumina template, namely an AAO-based template (i.e., porous alumina template 8). The pore size of the porous alumina template 8 is 500-5000nm, preferably 1000nm.

[0039] S7: Grind the AAO-based template by mechanical crushing or ball milling for 30 minutes to obtain a porous AAO-based material with a thickness of about 100-1000 μm, preferably 200 μm.

[0040] S8: AAO-based materials (i.e., porous alumina template 8) of different thicknesses are coupled to the optical fiber end 9 using a silane coupling agent to produce gradient-differentiated AAO-based optical fiber output ends 7 with AAO-based thicknesses of approximately 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 μm, respectively, so that the intensity of natural light passing through the AAO-based material can be adjusted to meet the lighting requirements of zebrafish growth and development at a specific stage.

[0041] Furthermore, the AAO-based optical fiber output end 7 can change the intensity, position and depth of natural light according to specific experimental requirements, wherein the position of the output light is jointly controlled by the optical fiber control slide 10, the converter 11 and the intelligent controller 12. The portion of the light-guiding optical fiber 6 in the zebrafish constant temperature culture tank 13 is inserted into the optical fiber control slide 10, and the intelligent controller 12 controls the lateral and longitudinal movement of the light-guiding optical fiber 6 in the optical fiber control slide 10, thereby realizing point light source irradiation of the AAO-based optical fiber output end 7 at different positions to meet the needs of zebrafish behavioral tests (such as light avoidance, sinking to the bottom experiment, etc.); the intelligent controller 12 controls the converter 11 to extend the selected specific optical fiber end 9 from the circular hole of the converter 11, thereby switching the optical fiber end 9 coupled to porous alumina 8 of different sizes for illumination, thereby realizing the adjustment of light intensity;

[0042] Furthermore, the intelligent controller 12 controls the AAO-based optical fiber output end 7 to extend and retract up and down and probe into the water. After entering the water body, the light will continue to weaken with the depth, and the light intensity will continue to decrease, which is also called the light attenuation phenomenon. When the water body is polluted and the water quality environment changes, the transparency of the water body changes accordingly, thereby interfering with the underwater light attenuation process, and ultimately affecting the light field in which the aquatic organisms are located. The AAO-based optical fiber output end 7 introduces natural light below the water surface A, which can reduce the impact of water quality transparency and changes in the water surface on the lighting of underwater organisms and ensure the lighting needs.

[0043] Furthermore, two underwater illuminance meters 16 are installed on the inner wall of the zebrafish constant temperature culture tank 13, which are used to detect underwater light at different depths. The specific fixed depth can be determined according to experimental requirements; the underwater illuminance meter 16 is connected to the constant temperature control circuit board 15. In the context of wireless network communication, the underwater illuminance meter 16 can transmit the illuminance data to the computer in real time, and then the correlation between the underwater illuminance change and the growth and development of zebrafish can be analyzed.

[0044] Furthermore, the zebrafish constant temperature culture water tank 13 includes an inner shell and an outer shell, and a heating pipe 17 and a heat preservation material are laid in the gap between the inner and outer shells. The heating pipe 17 is mainly laid on the side wall and the bottom of the zebrafish constant temperature culture water tank 13; the inner and outer shells of the zebrafish constant temperature culture water tank 13 are made of stainless steel, and the surface of the outer shell is covered with a heat preservation and heat insulation material; one end of the heating pipe 17 receives the energy transmission of the small solar panel 4, and the other end is connected to the electronic thermometer 14, and is controlled by the constant temperature control circuit board 15 and the intelligent controller 12; the electronic thermometer 14 is fixed below the water surface A of the inner wall of the zebrafish constant temperature culture water tank 13, and is used to detect the temperature of the zebrafish breeding water in real time. If the temperature is lower than the suitable temperature range, the heating pipe 17 will start and heat; if the temperature is higher than the suitable temperature range, the heating pipe will be closed until the water temperature cools down.

[0045] Furthermore, the outer periphery of the optical fiber conductor 6 is wrapped with a stainless steel pipe and an outer protective cover to prevent rainwater corrosion and ensure circuit safety.

[0046] Table 1 below shows the light intensity comparison between using the module and not using it:

[0047] Table 1

[0048]

[0049] Embodiment 2

[0050] The second embodiment is based on the first embodiment and is used to dynamically detect the potential health hazards of nighttime light pollution to organisms. The excessive quantification of street lights and commercial signs in cities makes the nights as bright as day. The potential risks of nighttime light pollution to the ecology and human health have become a research hotspot in recent years. Compared with the nocturnal mouse model, zebrafish, as a diurnal animal, its related toxicology experiments are considered to have unique reference value for human health. However, existing zebrafish lighting devices often have defects such as extreme lighting settings and fixed lighting without dynamic changes, and cannot accurately simulate urban nighttime light pollution scenes. The AAO-based natural light water environment introduction module of the present invention can specifically solve this technical problem in this embodiment.

[0051] In the second embodiment, the intelligent controller 12 can control the switch of the entire device and is connected to the light sensor 2. When the ambient light perceived by the light sensor 2 is lower than the set threshold, it is determined that the external environment has entered the night from the day. On the contrary, when it exceeds the threshold again, it enters the day from the night. Further, in order to introduce the night ambient light, the intelligent controller 12 controls the device to keep working, that is, the natural light receiving end 5 collects the night light and introduces the dynamic night light pollution situation into the zebrafish constant temperature culture tank 13 through the light guide fiber 6 to achieve light pollution exposure. However, when the purpose is to cultivate zebrafish normally as described in Example 1, the device can be turned off at night by the intelligent controller 12, so that the light sensor 2 stops collecting light after sensing that the light enters the night state, thereby protecting the growth and development of zebrafish from the interference of night light pollution while ensuring natural light during the day.

[0052] The present invention uses natural light optical fiber to introduce zebrafish into the laboratory to simulate the real light environment of the outside world. At the same time, the natural light is made adjustable through the preparation of AAO-based materials, thereby serving various biomedical and environmental toxicology experiments based on the zebrafish model.

[0053] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0054] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0055] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An AAO-based natural light water environment introduction module, characterized in that: The invention comprises a natural light receiving end (5), an optical fiber introduction device and a zebrafish constant temperature culture water tank (13); the natural light receiving end (5) comprises a focusing lens (3) with an upper opening and a small solar panel (4); the small solar panel (4) is arranged at the center of the bottom of the focusing lens (3) with an upper opening; the small solar panel (4) is connected to the zebrafish constant temperature culture water tank (13) through the optical fiber introduction device, one end of the optical fiber introduction device is arranged under the water surface of the zebrafish constant temperature culture water tank (13) to introduce natural light into the zebrafish constant temperature culture water tank (13); the optical fiber introduction device comprises a light guide optical fiber (6), a converter (11) and an AAO-based optical fiber output end (7); the input end of the light guide optical fiber (6) is connected to the focusing lens (3), and the output end of the light guide optical fiber (6) extends to the inside of the zebrafish constant temperature culture water tank and is connected to the AAO-based optical fiber output end (7) through the converter (11) and the optical fiber end (9).

2. The AAO-based natural light water environment introduction module according to claim 1, characterized in that: The natural light receiving end (5) further comprises a rotation tracking mechanism (1) and a light sensor (2); the rotation tracking mechanism (1) is arranged at the bottom of the focusing lens (3), the light sensor (2) is arranged at the center of the focusing lens (3) and is located above the rotation tracking mechanism (1), the rotation tracking mechanism (1) and the light sensor (2) are connected, the small solar panel (4) is arranged between the rotation tracking mechanism (1) and the light sensor (2), and the light sensor (2) senses the sun's position in real time and drives the rotation tracking mechanism (1) to directionally track natural light.

3. The AAO-based natural light water environment introduction module according to claim 1, characterized in that: The AAO-based optical fiber output end (7) comprises a porous alumina template (8), and the porous alumina template (8) is coupled to the optical fiber end (9).

4. The AAO-based natural light water environment introduction module according to claim 3, characterized in that: The pore size and thickness of the porous alumina template (8) can be replaced to adjust the intensity of the introduced natural light.

5. The AAO-based natural light water environment introduction module according to claim 4, characterized in that: The pore size of the porous alumina template (8) is 500-5000 nm.

6. The AAO-based natural light water environment introduction module according to claim 1, characterized in that: The optical fiber introduction device further comprises an optical fiber regulating slide rail (10), wherein the optical fiber regulating slide rail (10) is connected to a light-guiding optical fiber (6) in a zebrafish constant temperature culture water tank (13); an intelligent controller (12) is arranged on the top of the zebrafish constant temperature culture water tank (13), and the intelligent controller (12) controls the light-guiding optical fiber (6) to move in the optical fiber regulating slide rail (10), thereby changing the horizontal and vertical positions of the AAO-based natural light output end (7) in water, as well as the underwater depth.

7. The AAO-based natural light water environment introduction module according to claim 6, characterized in that: An electronic thermometer (14) is provided on the inner wall of the zebrafish constant temperature culture water tank (13), and the electronic thermometer (14) is arranged below the water surface.

8. The AAO-based natural light water environment introduction module according to claim 7, characterized in that: The zebrafish constant temperature culture water tank (13) comprises an inner shell and an outer shell, wherein a heating pipe (17) and a heat preservation material are arranged between the inner shell and the outer shell; one end of the heating pipe (17) is connected to a small solar panel (4) through a circuit for receiving energy transmission from the small solar panel (4), and the other end of the heating pipe (17) is connected to an electronic thermometer (14); a constant temperature control circuit board (15) is arranged on one side of the zebrafish constant temperature culture water tank (13), and the heating pipe (17) is connected to the constant temperature control circuit board (15) and the power supply control of the intelligent controller (12).

9. The AAO-based natural light water environment introduction module according to claim 7, characterized in that: At least two underwater illuminators (16) are arranged on the side wall of the zebrafish constant temperature culture tank (13); the constant temperature control circuit board (15) is connected to the power supply of the underwater illuminator (16); the underwater illuminator (16) is connected to a remote computer via wireless; the underwater illuminator (16) is used to detect underwater illumination at different depths and transmit illumination data to the computer in real time.

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