A sample analysis method and apparatus

Abstract

A sample analysis method and apparatus involves preparing a test sample using samples and reagents, irradiating the test sample with light of multiple wavelengths and intensities to obtain optical information corresponding to the multiple wavelengths and intensities of light, and selecting optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to the multiple wavelengths and intensities of light to analyze the sample based on the content of interfering substances in the sample.

Description

Technical Field

[0001] This invention relates to a sample analysis method and apparatus. Background Technology

[0002] Sample analysis devices are used to analyze the biochemical characteristics of samples. They are widely used in clinical medicine to help medical personnel diagnose patients' conditions. Taking a blood coagulation analyzer as an example, it can measure blood clotting time and the concentration or activity of related substances. Blood coagulation analyzers can use optical methods to detect coagulation parameters. Specifically, the analyzer irradiates the solution in the reaction vessel during the reaction process with light and analyzes the scattered or transmitted light to obtain optical information such as the absorbance of the solution, thereby obtaining information such as clotting time or the concentration of the analyte.

[0003] Optical methods detect coagulation parameters by detecting the optical information of light scattering, reflection, or transmission through the reaction solution. Therefore, when there are interfering substances in the sample that change the properties of light scattering, reflection, or transmission through the reaction solution, it will affect the measurement, making the test results inaccurate. In severe cases, no test results may be obtained at all. Invention Overview

[0005] Technical issues

[0006] This invention mainly provides a sample analysis method and apparatus.

[0007] Solution to the problem

[0008] Technical solutions

[0009] According to the first aspect, one embodiment provides a sample analysis method, comprising:

[0010] Prepare the required test samples using sample and reagent preparation;

[0011] The test sample is irradiated with light of various wavelengths and intensities to obtain optical information corresponding to the various wavelengths and intensities of light;

[0012] Based on the interference content of the sample, the optical information corresponding to a specific wavelength and intensity of light is selected from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample.

[0013] In one embodiment, the step of selecting optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to multiple wavelengths and multiple intensities of light to analyze the sample based on the content of interfering substances in the sample includes:

[0014] The test items for measuring the sample correspond to the matching dominant wavelength and dominant intensity of light;

[0015] When the content of interfering substances in the sample is less than the first threshold, the optical information corresponding to the dominant wavelength and dominant intensity of light corresponding to the test item is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0016] When the content of interfering substances in the sample is greater than the first threshold, the optical information corresponding to the main wavelength and / or intensity of the light corresponding to the item being measured is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0017] In one embodiment, when the content of interfering substances in the sample is greater than a first threshold and less than a second threshold, the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item being measured and an intensity equal to the dominant intensity corresponding to the item being measured is selected from the optical information corresponding to light with multiple wavelengths and multiple intensities to analyze the sample.

[0018] When the content of interfering substances in the sample is greater than the second threshold, the optical information corresponding to light with a wavelength greater than the dominant wavelength and intensity of the item being measured is selected from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample.

[0019] In one embodiment, when the content of interfering substances in the sample is greater than a first threshold, it is further determined whether the item of the test sample supports the measurement of non-dominant wavelength light. If it does not support it, the optical information corresponding to light with a wavelength equal to the dominant wavelength of the item of the test sample and an intensity greater than the dominant intensity of the item of the test sample is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0020] In one embodiment, the multiple wavelengths include: a first dominant wavelength (405 nm) corresponding to the items measured by the chromogenic substrate method, a second dominant wavelength (575 nm) corresponding to the items measured by the immunoturbidimetric method, a third dominant wavelength (660 nm) corresponding to the items measured by the coagulation method, and a fourth wavelength (800 nm) greater than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

[0021] In one embodiment, the plurality of strengths includes at least a first strength and a second strength greater than the first strength.

[0022] In one embodiment, the light with multiple wavelengths and multiple intensities includes: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, and light having a fourth wavelength and a second intensity.

[0023] According to a second aspect, one embodiment provides a sample analysis apparatus, comprising:

[0024] Preparation components are used to prepare the test samples required for the project using samples and reagents;

[0025] A light-illuminating component for illuminating the test sample with light of various wavelengths and intensities;

[0026] The light-receiving component is used to receive the output light signal of the test sample after it is irradiated by the light-illuminating component, so as to obtain optical information corresponding to the multiple wavelengths and multiple intensities of light;

[0027] An analysis component is used to analyze the sample by selecting optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to multiple wavelengths and multiple intensities of light, based on the content of interfering substances in the sample.

[0028] In one embodiment, the test items for measuring the sample correspond to light with matching dominant wavelength and dominant intensity;

[0029] When the content of interfering substances in the sample is less than the first threshold, the analysis component selects the optical information corresponding to the dominant wavelength and dominant intensity of light corresponding to the test item from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample;

[0030] When the content of interfering substances in the sample is greater than the first threshold, the analysis component selects the optical information corresponding to the main wavelength and / or intensity of the light corresponding to the item being measured from the optical information corresponding to the light of the multiple wavelengths and multiple intensities, and analyzes the sample.

[0031] In one embodiment, when the content of interfering substances in the sample is greater than a first threshold and less than a second threshold, the analysis component selects the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item being measured and an intensity equal to the dominant intensity corresponding to the item being measured from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0032] When the content of interfering substances in the sample is greater than the second threshold, the analysis component selects the optical information corresponding to light with a wavelength greater than the dominant wavelength and intensity corresponding to the item being measured from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample.

[0033] In one embodiment, when the content of interfering substances in the sample is greater than a first threshold, the analysis component further determines whether the item being measured supports measurement with light of a non-dominant wavelength. If not, the analysis component selects from the optical information corresponding to the various wavelengths and intensities of light the optical information corresponding to light whose wavelength is equal to the dominant wavelength of the item being measured and whose intensity is greater than the dominant intensity of the item being measured to analyze the sample.

[0034] In one embodiment, the multiple wavelengths include: a first dominant wavelength (405 nm) corresponding to the items measured by the chromogenic substrate method, a second dominant wavelength (575 nm) corresponding to the items measured by the immunoturbidimetric method, a third dominant wavelength (660 nm) corresponding to the items measured by the coagulation method, and a fourth wavelength (800 nm) not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

[0035] In one embodiment, the plurality of strengths includes at least a first strength and a second strength greater than the first strength.

[0036] In one embodiment, the light with multiple wavelengths and multiple intensities includes: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, and light having a fourth wavelength and a second intensity.

[0037] According to a third aspect, one embodiment provides a computer-readable storage medium including a program that can be executed by a processor to implement the methods described in any of the embodiments herein.

[0038] Beneficial effects of the invention

[0039] Brief description of the accompanying drawings Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the absorption spectra of light in various wavelength ranges for the three interfering substances: hemoglobin, bilirubin, and chyle.

[0041] Figure 2 This is a schematic diagram of the transmission response optical curves of a normal sample and a severely chylous sample in one embodiment.

[0042] Figure 3 This is a schematic diagram of the structure of a sample analysis device according to one embodiment;

[0043] Figure 4 This is a schematic diagram of the sample analysis device according to another embodiment;

[0044] Figure 5 A schematic diagram of the light provided by a lighting component during a lighting cycle;

[0045] Figure 6 Another schematic diagram of the light provided by the lighting component during the lighting cycle;

[0046] Figure 7 This is a schematic diagram of the structure of a lighting component according to one embodiment;

[0047] Figure 8 This is a schematic diagram of the structure of a lighting component according to another embodiment;

[0048] Figure 9 This is a schematic diagram of the structure of a lighting component according to another embodiment;

[0049] Figure 10 A schematic diagram of the structure of a lighting component according to another embodiment;

[0050] Figure 11 This is a schematic diagram of the sample analysis device according to another embodiment;

[0051] Figure 12 This is a flowchart illustrating a sample analysis method according to one embodiment;

[0052] Figure 13 This is a flowchart illustrating a sample analysis method according to another embodiment.

[0053] Invention Embodiments

[0054] Embodiments of the present invention

[0055] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0056] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0057] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0058] Optical methods for coagulation testing can generally be divided into three types: coagulation assays, immunoturbidimetric assays, and chromogenic substrate assays. Chromogenic substrate assays typically use violet or ultraviolet light (340nm-420nm) and are commonly used to measure parameters such as antithrombin-III (AT-III or AT3). Immunoturbidimetric assays typically use yellow-green light (520nm-590nm) and are commonly used to measure parameters such as D-dimer (DD) and fibrin / fibrinogen degradation products (FDP). Coagulation assays typically use red or infrared light (660nm-800nm) and are commonly used to measure parameters such as thrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB).

[0059] Regardless of whether it's a coagulation method, immunoturbidimetric assay, or chromogenic substrate method, in optical coagulation measurements, the presence of interfering substances in the sample can interfere with the detection. Normal plasma samples are generally pale yellow and almost transparent. However, some patients, due to illness or other reasons, experience symptoms such as jaundice, hemolysis, or lipemia, resulting in plasma that is brownish-yellow, slightly red, or milky white. Jaundice indicates the presence of bilirubin as an interfering substance in the sample; hemolysis indicates the presence of hemoglobin; and lipemia indicates the presence of chyle. These three interfering substances have different absorption spectra. When plasma samples contain interfering substances such as hemoglobin, bilirubin, and chyle, these substances have strong light absorption, thus interfering with the sample detection and causing deviations in the results. Hemoglobin, bilirubin, and chyle can generally be collectively referred to as HIL interference, where H stands for hemoglobin, I for bilirubin, and L for chyle. These three interfering substances exhibit different colors, and their absorption spectra are as follows: Figure 1As shown, bilirubin and hemoglobin exhibit distinct absorption peaks—bilirubin has a strong absorption peak around 450 nm, and hemoglobin has a strong absorption peak around 420 nm. Both show almost no absorption above 660 nm. Chyle, on the other hand, shows absorbance across the entire visible spectrum, with absorbance decreasing at increasingly larger wavelengths, although it still exhibits some absorbance even at 800 nm. Therefore, as can be seen from the figure, these three interfering substances have strong absorption in small wavelength ranges—especially below 600 nm. This significantly reduces the transmittance of the sample-reagent mixture, resulting in very little actually received light, affecting the accuracy and reliability of optical measurements. Sometimes, the received light is almost zero, making it impossible to identify the reaction process between the sample and the reagent.

[0060] One solution to address interfering substances is to use light in wavelengths that are not absorbed by these substances in the sample. For example, using a longer wavelength like 800nm ​​to illuminate the mixture of sample and reagent. The graph clearly shows that hemoglobin and bilirubin absorb almost no light with wavelengths greater than 800nm, while chyle also shows relatively little absorption. This approach effectively eliminates the influence of bilirubin and hemoglobin on sample measurements. However, it still has an impact on chyle samples, especially retested chyle samples. Although chyle absorbs relatively little light with wavelengths greater than 800nm, this absorption is still significant for applications requiring high accuracy. Furthermore, when the concentration of chyle in the sample is high, even in applications with moderate accuracy requirements (such as medical examinations), this absorption remains substantial and can severely affect the accuracy of the results. The transmission response optical curves for normal and severely chyle samples are shown below. Figure 2 As shown, Figure 2 The horizontal axis represents time in seconds, and the vertical axis represents the luminous flux received by the transmission photodetector. Figure 2 As can be seen, for samples with severe chyle, due to their low transmittance, the light flux was almost zero throughout the reaction, with virtually no light transmission—meaning that the absorbance of the analyte was too high, exceeding the maximum absorbance test range of the optical sensor.

[0061] Furthermore, the aforementioned method of switching to larger wavelengths of light for measurement is not suitable for items such as those using chromogenic substrate methods. This is because, from the perspective of the detection principle, the chromogenic substrate method relies on the reaction between the sample and the detection reagent, after which the reagent displaces substances from the sample. These displaced substances only absorb in the ultraviolet and violet light range. Therefore, generally only the aforementioned 340nm-420nm violet or ultraviolet light can be used, and other wavelengths of light cannot be used. In contrast, coagulation and immunoturbidimetric methods, in addition to using the aforementioned wavelength ranges of light, can theoretically use other wavelength ranges of light for detection.

[0062] The applicant has researched and improved upon the above-mentioned issues. The applicant proposed increasing light intensity to address the impact of interfering substances on optical detection. Depending on the concentration of interfering substances in the sample, switching to a larger wavelength and / or increasing light intensity can be used to mitigate the impact of interfering substances on sample detection. This will be explained in detail below.

[0063] Some embodiments of the present invention disclose a sample analysis device. A sample analysis device is an instrument used to analyze and measure samples. Taking a coagulation analyzer (i.e., a coagulation analyzer mentioned herein) as an example, the testing procedure of the sample analysis device will be illustrated. The testing procedure of a coagulation analyzer is generally as follows: A sample, such as blood or plasma, and a test reagent are added to a container, such as a reaction cup, to prepare a test sample (or mixture, reaction solution, etc.). After incubating the test sample, the reaction cup is placed in a preset position, such as the sample detection position. The coagulation analyzer can irradiate the test sample in the reaction cup with light of, for example, multiple wavelengths, and analyze it using methods such as coagulation, immunoturbidimetry, or chromogenic substrate methods to obtain the coagulation reaction curve of the test sample over time, thereby further calculating the coagulation time or other coagulation-related performance parameters of the test sample.

[0064] Please refer to Figure 3 In some embodiments, the sample analysis apparatus includes a preparation component 10, an illumination component 30, a light-receiving component 50, and an analysis component 70, which are described in detail below.

[0065] Preparation component 10 is used to prepare the test samples required for the project by means of samples and reagents.

[0066] Figure 4 In one embodiment of the preparation component 10, the preparation component 10 may include a sample carrying component 11, a sample dispensing mechanism 12, a reagent carrying component 13, a reagent dispensing mechanism 14, and a reaction component 15. The sample carrying component 11 is used to carry samples. In some examples, the sample carrying component 11 may include a sample delivery module (SDM) and a front-end track; in other examples, the sample carrying component 11 may also be a sample tray—for example... Figure 4 In such an example, a sample tray may include multiple sample positions for placing items such as reaction cups. By rotating its disc-shaped structure, the sample tray can be positioned accordingly, for example, for the sample dispensing mechanism 12 to pick up the sample. The sample dispensing mechanism 12 is used to pick up the sample and dispense it into the reaction cup to be added. For example, the sample dispensing mechanism 12 may include a sample needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, thereby moving the sample needle to pick up the sample carried by the sample carrier 11, and to move to the reaction cup to be added, and to dispense the sample into the reaction cup. The reagent carrier 13 is used to carry reagents. In one embodiment, the reagent carrier 13 may be a reagent tray, which is arranged in a disc-shaped structure and has multiple positions for carrying reagent containers. The reagent carrier 13 can rotate and drive the reagent containers it carries to rotate, for rotating the reagent containers to a specific position, for example, the position where the reagent dispensing mechanism 14 picks up the reagent. The number of reagent carriers 13 may be one or more. The reagent dispensing mechanism 14 is used to pick up the reagent and dispense it into the reaction cup to be added. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle, which moves in two or three dimensions through a two-dimensional or three-dimensional driving mechanism. This allows the needle to move to draw reagents from the reagent carrier 13, move to the reaction cup containing the reagent, and dispense the reagent into the reaction cup. The reaction component 15 holds a container (e.g., a reaction cup) containing a test sample prepared from a sample and detection reagents. In one example, the reaction component 15 is arranged in a disk-like structure with multiple placement positions for placing, for example, reaction cups. The reaction component 15 is rotatable and drives the reaction cups in the placement positions to rotate, thereby distributing the reaction cups and the mixture within them within the reaction disk. The incubated test sample is then directed to a sample detection position for optical detection. The sample detection position may be located on the reaction component 15, i.e., some placement positions on the reaction component 15 are sample detection positions; alternatively, the sample detection position may be located independently of the reaction component 15, i.e., at a location, for example, close to the reaction component 15. There may be one or more sample detection positions.

[0067] The illumination component 30 is used to irradiate the test sample prepared by the preparation component 10 with light of various wavelengths and intensities. The various wavelengths and intensities of light mentioned above will be described below.

[0068] In some embodiments, the aforementioned multiple wavelengths include: a first dominant wavelength corresponding to items measured by the chromogenic substrate method, a second dominant wavelength corresponding to items measured by the immunoturbidimetric method, a third dominant wavelength corresponding to items measured by the coagulation method, and a fourth wavelength not less than the first, second, and third dominant wavelengths. In some preferred embodiments, the range of the first dominant wavelength is 340nm-420nm, the range of the second dominant wavelength is 520nm-590nm, and the range of the third dominant wavelength is 660nm-800nm. In some embodiments, the fourth wavelength may be 800nm.

[0069] In some embodiments, the aforementioned multiple strengths include at least a first strength and a second strength greater than the first strength.

[0070] Therefore, in some embodiments, the light provided by the illumination component 30 includes light of multiple wavelengths and intensities, including: light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity. This light can be provided to the sample detection position in a time-division manner within one illumination cycle, thereby illuminating the sample to be measured. Figure 5 Here is an example where the first dominant wavelength is 405nm, the second dominant wavelength is 575nm, the third dominant wavelength is 660nm, and the fourth wavelength is 880nm.

[0071] In other embodiments, the illumination component 30 provides light of multiple wavelengths and intensities, including: light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, and light with a third dominant wavelength and a second intensity. This light can be provided to the sample detection position in a time-division manner within an illumination cycle to illuminate the sample for measurement. Figure 6 Here is an example where the first dominant wavelength is 405nm, the second dominant wavelength is 575nm, and the third dominant wavelength is 660nm.

[0072] For sample analysis devices with multiple sample detection positions, to simplify the structure, a multi-fiber bundle can be introduced to provide illumination to multiple sample detection positions. In some embodiments, please refer to... Figure 7The illumination component 30 may include a light source 31 and a multi-fiber splitter 39, so that the light source 31 can provide light to multiple sample detection positions simultaneously through the multi-fiber splitter 39. Specifically, the multi-fiber splitter 39 includes multiple optical fibers corresponding to multiple sample detection positions, and each optical fiber is used to provide light of multiple wavelengths and multiple intensities to the corresponding sample detection position.

[0073] Please refer to Figure 8 In some embodiments, the light source 31 may include a first light source 32, a second light source 33, and a third light source 34. The first light source 32 provides light of a first dominant wavelength, the second light source 33 provides light of a second dominant wavelength, and the third light source 34 provides light of a third dominant wavelength. The light source 31 may also include a fourth light source 35 for providing light of a fourth wavelength. Understandably, to improve light performance, some optical components for focusing, such as focusing lenses, may be added between the light source 31 and the multi-fiber bundle 39; collimating lenses may also be added between the multi-fiber bundle 39 and each sample detection position to improve the performance of light directed towards the sample detection position. In some embodiments, the illumination component 30 further includes a driving circuit 36, which is connected to the first light source 32, the second light source 33, the third light source 34, and the fourth light source 35. The driving circuit 36 ​​is used to provide a first driving current to drive the first light source 32, the second light source 33, the third light source 34, and the fourth light source 35 to generate light of a first intensity; and is also used to provide a first driving current to drive the first light source 32, the second light source 33, the third light source 34, and the fourth light source 35 to generate light of a second intensity, wherein the second driving current is greater than the first driving current. In this way, by providing different driving currents through the driving circuit 36 ​​to drive the first light source 32, the second light source 33, the third light source 34, and the fourth light source 35, the illumination component 30 can provide light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity within one illumination cycle.

[0074] Please refer to Figure 9In other embodiments, the light source 31 can also be implemented using a multi-wavelength light source 37 and a rotating filter 38. The multi-wavelength light source 37 provides light of multiple wavelengths, such as light of a first dominant wavelength, a second dominant wavelength, a third dominant wavelength, and a fourth wavelength. In some examples, the multi-wavelength light source 37 can be implemented using a halogen lamp. The rotating filter 38 includes a filter and an attenuator. The illumination component 30 is used to provide light of different wavelengths and intensities in a time-division manner as the rotating filter 38 rotates, for example, providing light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity. Then, the light can be provided to multiple sample detection positions through a multi-fiber bundle 59. Understandably, some optical components that improve the performance of the illumination light, such as lens groups, can also be added between the multi-wavelength light source 37 and the rotating filter 38.

[0075] The illumination period mentioned in this article can be 0.1s.

[0076] In some examples, the sample analysis device also provides an interference detection position, which will be further mentioned below. The illumination component 30 can also provide illumination to the interference detection position, for example, through a multi-fiber bundle 59, so that the light illuminating the sample detection position and the interference detection position by the illumination component 30 is the same. Figure 10 Here's an example. In the diagram, a violet LED provides 405nm light, a green LED provides 575nm light, a red LED provides 660nm light, and an infrared LED provides 800nm ​​light. These are combined through three dichroic mirrors and coupled together into the optical fiber of a multi-fiber bundle 59. The multi-fiber bundle 59 splits into multiple fiber ends. One of these fiber ends can be used to illuminate the second container (e.g., a cuvette or reaction vessel) at the interference detection site to detect interfering substances in the sample, such as their type and concentration. The remaining fiber ends are used to illuminate the container (e.g., a reaction vessel) containing the sample at the sample detection site. Of course, the LED light source in the diagram can be replaced with other types of light sources, such as an LD light source or a halogen lamp. In some examples, the illumination component 30 can also independently provide illumination to the sample detection site and the interference detection.

[0077] The above is a description of the illumination component 30. The light-receiving component 50 cooperates with the illumination component 30 and is used to receive the output light signal—such as reflected light, refracted light, and transmitted light—after the sample is illuminated by the illumination component 30, in order to obtain optical information corresponding to the various wavelengths and intensities of light. In one embodiment, the light-receiving component 50 receives light that has been illuminated by the illumination component 30 and transmitted through the sample, thereby obtaining optical information corresponding to the various wavelengths and intensities of light.

[0078] In some embodiments, the light-receiving component 50 can be implemented by an optoelectronic component capable of converting optical signals into electrical signals. Specifically, such an optoelectronic component can be a photodiode (PD), a photomultiplier tube (PMT), an avalanche photodiode (APD), a charge-coupled device (CCD), a complementary metal-oxide-semiconductor (CMOS), an image enhancement detector (ICCD), or an electron multiplier type (EMCCD). For a sample analysis device with multiple sample detection positions, one optoelectronic component can be provided for each sample detection position.

[0079] The analysis unit 70 is used to analyze the sample by selecting the optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to various wavelengths and intensities of light, based on the interfering substance content of the sample. This involves two aspects: first, how to obtain the interfering substance content of the sample; and second, how to select the optical information corresponding to a specific wavelength and intensity of light to analyze the sample, which will be explained below.

[0080] The following explains how to obtain and determine the content of interfering substances in a sample.

[0081] In this paper, the interference content of a sample can be determined using sample interference detection information. In some embodiments, the sample interference detection information includes at least one of the absorbance or luminous flux of the sample to be tested. The absorbance of the sample to be tested represents the degree to which the sample absorbs light when illuminated. If the absorbance of the sample to be tested at a preset wavelength exceeds a preset absorbance threshold, for example, the absorbance at at least one of 405nm, 575nm, 660nm, and 800nm ​​exceeds the corresponding absorbance threshold, it indicates that the interference in the sample exceeds the preset threshold, and anti-interference detection is required by increasing the light intensity or other methods. The luminous flux of the sample to be tested represents the degree to which light can pass through the sample when illuminated. The luminous flux of the sample to be tested can be the initial luminous flux detected before the formal coagulation test. If the initial luminous flux of the sample to be tested is lower than a preset luminous flux threshold, it indicates that the interference in the sample exceeds the preset threshold, and anti-interference detection is required by increasing the light intensity.

[0082] The following are some specific examples to illustrate this. Please refer to... Figure 11In some embodiments, the sample analysis apparatus may further include an interference detection component 80. In some embodiments, the interference detection component 80 includes at least one interference detection position and a detector 81 adjacent to the interference detection position. The detector 81 may be implemented by a component capable of converting optical signals into electrical signals, such as a photodetector, specifically a photodiode (PD), photomultiplier tube (PMT), avalanche photodiode (APD), charge-coupled device (CCD), complementary metal-oxide-semiconductor (CMOS), image enhancement detector (ICCD), or electron multiplier type detector (EMCCD). The illumination component 30 is used to illuminate a second container (e.g., a reaction cup or colorimetric cell) located at the interference detection position and containing at least one sample—for example, the illumination component 30 is illuminated by light of a first intensity; the detector 81 is used to receive the output light signal of the second container after it has been illuminated by the illumination component 30, to obtain interference detection information of the sample to be tested; this interference detection information is used to indicate the interference content of the sample to be tested.

[0083] By introducing the interference detection component 80, interference can be detected in the sample to be tested, and the interference detection information can be obtained. In some other embodiments, instead of setting up a separate interference detection position, the interference detection of the sample to be tested can be achieved at the same time as the sample detection position, which will be described in detail below.

[0084] In some embodiments, the illumination component 30 illuminates the container holding the test sample at the sample detection position with light of a first intensity. The light-receiving component 50 receives the output light signal of the container after being illuminated by the illumination component 30 to obtain the interference detection information of the sample to be tested. This interference detection information is used to determine the content of interference in the sample to be tested. Specifically, the interference detection information can be obtained by using the average luminous flux during the period from when the container holding the test sample is placed at the sample detection position to when the test begins. In one example, the mixing and movement of the mixture to the sample detection position can generally be completed within 3 seconds after the sample is added to the final trigger reagent. Then, the test begins at the 10th second. During the 7 seconds between the 3rd and 10th seconds, the illumination component 30 illuminates the container holding the test sample at the sample detection position with light of a first intensity. The light-receiving component 50 receives the output light signal of the container after being illuminated by the illumination component 30—for example, the average luminous flux and minimum transmittance during this period—to obtain the interference detection information of the sample.

[0085] The above are some embodiments of sample interference detection. It is understood that those skilled in the art can also use other methods to detect sample interference, such as taking a picture of the sample to be tested to obtain an image of the sample to be tested, and then analyzing the image through methods such as machine learning to obtain interference detection information of the sample to be tested.

[0086] The following explains how the analysis component 70 selects optical information analysis samples corresponding to light of a specific wavelength and intensity.

[0087] The test items for measuring the sample correspond to a matching dominant wavelength and dominant intensity of light. For example, the dominant wavelength matched by the chromogenic substrate method is the first dominant wavelength mentioned above, the dominant wavelength matched by the immunoturbidimetric method is the second dominant wavelength mentioned above, and the dominant wavelength matched by the coagulation method is the third dominant wavelength mentioned above, etc., and their dominant intensities are all the first intensity.

[0088] When the content of interfering substances in the sample is less than the first threshold, the analysis component 70 selects the optical information corresponding to the dominant wavelength and dominant intensity of light corresponding to the test item from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0089] When the content of interfering substances in the sample exceeds a first threshold, the analysis unit 70 selects the optical information corresponding to the dominant wavelength and / or intensity of light corresponding to the item being measured, which is greater than the dominant wavelength of the item being measured, from the optical information corresponding to the multiple wavelengths and intensities of light to analyze the sample. For example, taking an item measured by immunoturbidimetry as an example, when the content of interfering substances in the sample exceeds the first threshold, the analysis unit 70 can select the optical information corresponding to the fourth wavelength and first intensity of light to analyze the sample, or it can select the optical information corresponding to the second dominant wavelength and second intensity of light to analyze the sample, or even select the optical information corresponding to the fourth wavelength and second intensity of light to analyze the sample.

[0090] In some embodiments, when the content of interfering substances in a sample is greater than the first threshold, it can be further subdivided into two cases: the content of interfering substances in the sample is greater than the first threshold but less than the second threshold, and the content of interfering substances in the sample is greater than the second threshold. These cases will be explained in detail below.

[0091] When the content of interfering substances in the sample is greater than a first threshold but less than a second threshold, the analysis component 70 selects the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item being measured and an intensity equal to the dominant intensity corresponding to the item being measured, from the optical information corresponding to light of various wavelengths and intensities, to analyze the sample. Taking the item measured by immunoturbidimetry as an example, when the content of interfering substances in the sample is greater than the first threshold but less than the second threshold, the analysis component 70 can select the optical information corresponding to light of a fourth wavelength and a first intensity to analyze the sample.

[0092] When the content of interfering substances in the sample exceeds the second threshold, the analysis component 70 selects the optical information corresponding to light with a wavelength and intensity greater than the dominant wavelength and intensity corresponding to the item being measured from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. Taking the item measured by immunoturbidimetry as an example, when the content of interfering substances in the sample exceeds the second threshold, the analysis component 70 can select the optical information corresponding to light with a fourth wavelength and a second intensity to analyze the sample.

[0093] When the interference content of a sample is greater than the first threshold but less than the second threshold, the optical information analysis sample corresponding to the original light intensity and long wavelength is selected. When the interference content of a sample is greater than the second threshold, the optical information analysis sample corresponding to the high light intensity and long wavelength is selected. This is of practical significance because if the optical information analysis sample corresponding to the high light intensity and long wavelength is selected when the interference content of the sample is greater than the first threshold but less than the second threshold, the interference concentration of the sample is not very high at this time. Therefore, after the sample is irradiated by the high light intensity and long wavelength, the light signal received at the light receiving component 50 may be oversaturated, which will have an adverse effect on the analysis sample. Similarly, if the optical information analysis sample corresponding to the original light intensity and long wavelength is selected when the interference content of the sample is greater than the second threshold, the interference concentration of the sample is very high at this time. Therefore, after the sample is irradiated by the original light intensity and long wavelength, the light signal intensity received at the light receiving component 50 may be very low, which will also have an adverse effect on the analysis sample.

[0094] As mentioned above, some measurements do not support light measurements at wavelengths other than the dominant wavelength. Typically, for chromogenic substrate methods, measurements can only be performed using the first dominant wavelength and cannot be switched to a larger wavelength. Considering this, in some embodiments, when the interfering substance content of the sample exceeds a first threshold, the analysis unit 70 further determines whether the measurement of the sample supports non-dominant wavelength light. If not, the analysis unit 70 selects the optical information corresponding to light with a wavelength equal to the dominant wavelength of the measurement sample and an intensity greater than the dominant intensity of the measurement sample from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. Taking the chromogenic substrate method as an example again, when the interfering substance content of the sample exceeds the first threshold, the analysis unit 70 determines that the measurement does not support non-dominant wavelength light, and therefore the analysis unit 70 selects the optical information corresponding to light with a second wavelength and a second intensity to analyze the sample.

[0095] The above describes the sample analysis apparatus according to some embodiments of the present invention. A sample analysis method is also disclosed in some embodiments of the present invention.

[0096] Please refer to Figure 12 The sample analysis method in some embodiments includes the following steps:

[0097] Step 100: Prepare the test sample required for the project using the sample and reagents.

[0098] Step 200: Irradiate the test sample with light of various wavelengths and intensities to obtain optical information corresponding to the light of various wavelengths and intensities.

[0099] In some embodiments, the multiple wavelengths involved in step 200 include: a first dominant wavelength corresponding to the items measured by the chromogenic substrate method, a second dominant wavelength corresponding to the items measured by the immunoturbidimetric method, a third dominant wavelength corresponding to the items measured by the coagulation method, and a fourth wavelength not less than the first, second, and third dominant wavelengths. In some preferred embodiments, the range of the first dominant wavelength is 340nm-420nm, the range of the second dominant wavelength is 520nm-590nm, and the range of the third dominant wavelength is 660nm-800nm. In some embodiments, the fourth wavelength may be 800nm.

[0100] In some embodiments, the multiple strengths involved in step 200 include at least a first strength and a second strength greater than the first strength.

[0101] Therefore, in some embodiments, the multiple wavelengths and intensities of light provided in step 200 include: light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity. These lights can be provided to the sample detection position in a time-division manner within one illumination cycle, thereby illuminating the sample to be measured. As described above. Figure 5 This is one such example.

[0102] In other embodiments, the multiple wavelengths and intensities of light provided in step 200 include: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, and light having a third dominant wavelength and a second intensity. These lights can be provided to the sample detection position in a time-division manner within one illumination cycle, thereby illuminating the measurement sample.

[0103] Step 300: Based on the interfering substance content of the sample, select the optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. How to detect the interfering substance content of the sample has been explained in detail above and will not be repeated here. The following explains how step 300 selects the optical information corresponding to a specific wavelength and intensity of light to analyze the sample.

[0104] The test items for measuring the sample correspond to a matching dominant wavelength and dominant intensity of light. For example, the dominant wavelength matched by the chromogenic substrate method is the first dominant wavelength mentioned above, the dominant wavelength matched by the immunoturbidimetric method is the second dominant wavelength mentioned above, and the dominant wavelength matched by the coagulation method is the third dominant wavelength mentioned above, etc., and their dominant intensities are all the first intensity.

[0105] When the content of interfering substances in the sample is less than the first threshold, step 300 selects the optical information corresponding to the dominant wavelength and dominant intensity of light corresponding to the test item from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0106] When the content of interfering substances in the sample exceeds a first threshold, step 300 selects the optical information corresponding to the dominant wavelength and / or intensity of light corresponding to the item being measured from the optical information corresponding to the multiple wavelengths and intensities of light to analyze the sample. For example, taking an item measured by immunoturbidimetric assay as an example, when the content of interfering substances in the sample exceeds the first threshold, the analysis unit 70 can select the optical information corresponding to the fourth wavelength and first intensity of light to analyze the sample, or it can select the optical information corresponding to the second dominant wavelength and second intensity of light to analyze the sample, or even select the optical information corresponding to the fourth wavelength and second intensity of light to analyze the sample.

[0107] In some embodiments, when the content of interfering substances in a sample is greater than the first threshold, it can be further subdivided into two cases: the content of interfering substances in the sample is greater than the first threshold but less than the second threshold, and the content of interfering substances in the sample is greater than the second threshold. These cases will be explained in detail below.

[0108] When the content of interfering substances in the sample is greater than the first threshold but less than the second threshold, step 300 selects the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item being measured and an intensity equal to the dominant intensity corresponding to the item being measured from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. Taking the item measured by immunoturbidimetry as an example, when the content of interfering substances in the sample is greater than the first threshold but less than the second threshold, step 300 can select the optical information corresponding to light with a fourth wavelength and a first intensity to analyze the sample.

[0109] When the content of interfering substances in the sample exceeds the second threshold, step 300 selects the optical information corresponding to light with a wavelength and intensity greater than the dominant wavelength and intensity corresponding to the item being measured from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. Taking the item measured by immunoturbidimetry as an example, when the content of interfering substances in the sample exceeds the second threshold, step 300 can select the optical information corresponding to light with a fourth wavelength and a second intensity to analyze the sample.

[0110] When the interference content of a sample is greater than the first threshold but less than the second threshold, the optical information analysis sample corresponding to the original light intensity and long wavelength is selected. When the interference content of a sample is greater than the second threshold, the optical information analysis sample corresponding to the high light intensity and long wavelength is selected. This is of practical significance because if the optical information analysis sample corresponding to the high light intensity and long wavelength is selected when the interference content of the sample is greater than the first threshold but less than the second threshold, the interference concentration of the sample is not very high at this time. Therefore, after the sample is irradiated by the high light intensity and long wavelength, the light signal received at the light receiving component 50 may be oversaturated, which will have an adverse effect on the analysis sample. Similarly, if the optical information analysis sample corresponding to the original light intensity and long wavelength is selected when the interference content of the sample is greater than the second threshold, the interference concentration of the sample is very high at this time. Therefore, after the sample is irradiated by the original light intensity and long wavelength, the light signal intensity received at the light receiving component 50 may be very low, which will also have an adverse effect on the analysis sample.

[0111] As mentioned above, some measurements do not support light measurements at wavelengths other than the dominant wavelength. Typically, for chromogenic substrate methods, measurements can only be performed using the first dominant wavelength and cannot be switched to a larger wavelength. Considering this, in some embodiments, when the interfering substance content of the sample exceeds a first threshold, step 300 further determines whether the measurement of the sample supports non-dominant wavelength light. If not, step 300 selects the optical information corresponding to light with a wavelength equal to the dominant wavelength of the measurement sample and an intensity greater than the dominant intensity of the measurement sample from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. Taking the chromogenic substrate method as an example again, when the interfering substance content of the sample exceeds the first threshold, step 300 determines that the measurement does not support non-dominant wavelength light, therefore step 300 selects the optical information corresponding to light with a second wavelength and a second intensity to analyze the sample.

[0112] In summary, please refer to Figure 13 In one specific embodiment of the sample analysis method, it may include the following steps:

[0113] Step 100: Prepare the test sample required for the project using the sample and reagents.

[0114] Step 200: Irradiate the test sample with light of various wavelengths and intensities to obtain optical information corresponding to the light of various wavelengths and intensities.

[0115] Step 310: Determine whether the content of interfering substances in the sample is less than the first threshold.

[0116] Step 312: When the content of interfering substances in the sample is less than the first threshold, the optical information corresponding to the dominant wavelength and dominant intensity of light corresponding to the various wavelengths and intensities of light is selected from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample.

[0117] Step 314: When the content of interfering substances in the sample is greater than the first threshold, continue to determine whether the test items of the sample support the measurement of non-dominant wavelength light. If the test items of the sample do not support the measurement of non-dominant wavelength light, proceed to step 316; otherwise, if the test items of the sample support the measurement of non-dominant wavelength light, proceed to step 318.

[0118] Step 316: When the test item does not support the measurement of non-dominant wavelength light, the optical information corresponding to light with a wavelength equal to the dominant wavelength of the test item and an intensity greater than the dominant intensity of the test item is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

[0119] Step 318: If the test item of the sample supports the measurement of non-dominant wavelength light, then further determine whether the content of interfering substances in the sample is greater than the second threshold.

[0120] Step 320: When the content of interfering substances in the sample is not greater than the second threshold, the optical information corresponding to the light with a wavelength greater than the dominant wavelength of the item being measured and an intensity equal to the dominant intensity of the item being measured is selected from the optical information corresponding to the light with multiple wavelengths and multiple intensities to analyze the sample.

[0121] Step 322: When the content of interfering substances in the sample is greater than the second threshold, the optical information corresponding to the light with a wavelength greater than the main wavelength and intensity corresponding to the item being measured is selected from the optical information corresponding to the light with multiple wavelengths and multiple intensities to analyze the sample.

[0122] This document describes various exemplary embodiments with reference to them. However, those skilled in the art will recognize that changes and modifications can be made to the exemplary embodiments without departing from the scope of this document. For example, various operational steps and components for performing operational steps can be implemented in different ways depending on the specific application or considering any number of cost functions associated with the operation of the system (e.g., one or more steps can be deleted, modified, or combined with other steps).

[0123] In the above embodiments, implementation can be achieved, in whole or in part, by software, hardware, firmware, or any combination thereof. Furthermore, as those skilled in the art will understand, the principles herein can be reflected in a computer program product on a computer-readable storage medium pre-loaded with computer-readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-ROMs, DVDs, Blu-ray discs, etc.), flash memory, and / or the like. These computer program instructions can be loaded onto a general-purpose computer, special-purpose computer, or other programmable data processing apparatus to form a machine, such that instructions executing on the computer or other programmable data processing apparatus can generate means for implementing a specified function. These computer program instructions can also be stored in a computer-readable storage medium that can instruct the computer or other programmable data processing apparatus to operate in a particular manner, such that instructions stored in the computer-readable storage medium can form an article of manufacture including means for implementing the specified function. The computer program instructions can also be loaded onto a computer or other programmable data processing apparatus to perform a series of operational steps on the computer or other programmable apparatus to produce a computer-implemented process, such that instructions executing on the computer or other programmable apparatus can provide steps for implementing the specified function.

[0124] While the principles herein have been illustrated in various embodiments, numerous modifications to the structure, arrangement, proportions, elements, materials, and components, particularly suited to specific environmental and operational requirements, may be used without departing from the principles and scope of this disclosure. These modifications and other alterations or alterations will be included within the scope of this document.

[0125] The foregoing specific descriptions have been described with reference to various embodiments. However, those skilled in the art will recognize that various modifications and changes can be made without departing from the scope of this disclosure. Therefore, considerations for this disclosure are to be illustrative rather than restrictive, and all such modifications are to be included within its scope. Similarly, advantages, other advantages, and solutions to problems with respect to various embodiments have been described above. However, benefits, advantages, solutions to problems, and any elements that produce these, or make them more explicit, should not be construed as critical, essential, or necessary. The term “comprising” and any other variations thereof as used herein are non-exclusive inclusion, meaning that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed or not part of the process, method, system, article, or apparatus. Furthermore, the term “coupled” and any other variations thereof as used herein refer to physical connections, electrical connections, magnetic connections, optical connections, communication connections, functional connections, and / or any other connections.

[0126] Those skilled in the art will recognize that many changes can be made to the details of the above embodiments without departing from the basic principles of the invention. Therefore, the scope of the invention should be determined only by the claims.

Claims

1. A sample analysis method for optical determination of coagulation; characterized in that, The sample analysis method includes: The required assay samples for the project are prepared by means of samples and reagents, including plasma; The test sample is irradiated with light of various wavelengths and intensities to obtain optical information corresponding to the various wavelengths and intensities of light; the test item of the test sample corresponds to a matching dominant wavelength and dominant intensity of light; Based on the content of interfering substances in the sample, the optical information corresponding to a specific wavelength and intensity of light is selected from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample. The interfering substances include at least one of hemoglobin, bilirubin, and chyle. in: When the content of interfering substances in the sample is less than the first threshold, the optical information corresponding to the dominant wavelength and dominant intensity of the test sample is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample. When the content of interfering substances in the sample is greater than the first threshold and less than the second threshold, the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item of the test sample and an intensity equal to the dominant intensity corresponding to the item of the test sample is selected from the optical information corresponding to light with multiple wavelengths and multiple intensities to analyze the sample; when the content of interfering substances in the sample is greater than the second threshold, the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item of the test sample and an intensity also greater than the dominant intensity corresponding to the item of the test sample is selected from the optical information corresponding to light with multiple wavelengths and multiple intensities to analyze the sample.

2. The sample analysis method as described in claim 1, characterized in that, When the content of interfering substances in the sample is greater than the first threshold, it is also determined whether the item of the sample can be measured by non-dominant wavelength light. If it cannot be measured, the optical information corresponding to light with a wavelength equal to the dominant wavelength of the item of the sample and an intensity greater than the dominant intensity of the item of the sample is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample.

3. The sample analysis method as described in claim 1, characterized in that, The multiple wavelengths include: the first dominant wavelength corresponding to the items measured by the chromogenic substrate method, the second dominant wavelength corresponding to the items measured by the immunoturbidimetric method, the third dominant wavelength corresponding to the items measured by the coagulation method, and a fourth wavelength greater than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

4. The sample analysis method as described in claim 1 or 3, characterized in that, The multiple strengths include at least a first strength and a second strength greater than the first strength.

5. The sample analysis method as described in claim 4, characterized in that, The light with multiple wavelengths and multiple intensities includes: light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity.

6. A sample analysis device for optical determination of coagulation; characterized in that, The sample analysis device includes: Preparation components for preparing assay samples required for a project using samples and reagents, said samples including plasma; An illumination component for illuminating the test sample with light of various wavelengths and intensities; the test sample corresponds to a main wavelength and main intensity of light. The light-receiving component is used to receive the output light signal of the test sample after it is irradiated by the light-illuminating component, so as to obtain optical information corresponding to the multiple wavelengths and multiple intensities of light; An analysis component is used to analyze the sample by selecting optical information corresponding to a specific wavelength and intensity of light from the optical information corresponding to multiple wavelengths and multiple intensities of light, based on the content of interfering substances in the sample, wherein the interfering substances include at least one of hemoglobin, bilirubin, and chyle; in: When the content of interfering substances in the sample is less than the first threshold, the optical information corresponding to the dominant wavelength and dominant intensity of the test sample is selected from the optical information corresponding to the multiple wavelengths and multiple intensities of light to analyze the sample. When the content of interfering substances in the sample is greater than the first threshold and less than the second threshold, the analysis component selects the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item of the test sample and an intensity equal to the dominant intensity corresponding to the item of the test sample from the optical information corresponding to light with multiple wavelengths and multiple intensities to analyze the sample; when the content of interfering substances in the sample is greater than the second threshold, the analysis component selects the optical information corresponding to light with a wavelength greater than the dominant wavelength corresponding to the item of the test sample and an intensity also greater than the dominant intensity corresponding to the item of the test sample from the optical information corresponding to light with multiple wavelengths and multiple intensities to analyze the sample.

7. The sample analysis apparatus as described in claim 6, characterized in that, When the content of interfering substances in the sample is greater than the first threshold, the analysis component further determines whether the item being measured supports measurement with light of a non-dominant wavelength. If not, the analysis component selects the optical information corresponding to light with a wavelength equal to the dominant wavelength of the item being measured and an intensity greater than the dominant intensity of the item being measured from the optical information corresponding to the various wavelengths and intensities of light to analyze the sample.

8. The sample analysis apparatus as described in claim 6, characterized in that, The multiple wavelengths include: the first dominant wavelength corresponding to the items measured by the chromogenic substrate method, the second dominant wavelength corresponding to the items measured by the immunoturbidimetric method, the third dominant wavelength corresponding to the items measured by the coagulation method, and a fourth wavelength not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

9. The sample analysis apparatus as described in claim 6 or 8, characterized in that, The multiple strengths include at least a first strength and a second strength greater than the first strength.

10. The sample analysis apparatus as described in claim 9, characterized in that, The light with multiple wavelengths and multiple intensities includes: light with a first dominant wavelength and a first intensity, light with a second dominant wavelength and a first intensity, light with a third dominant wavelength and a first intensity, light with a fourth wavelength and a first intensity, light with a first dominant wavelength and a second intensity, light with a second dominant wavelength and a second intensity, light with a third dominant wavelength and a second intensity, and light with a fourth wavelength and a second intensity.

11. A computer-readable storage medium, characterized in that, Includes a program that can be executed by a processor to implement the method as described in any one of claims 1 to 5.

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