Sample filling device, method and analyzer

Abstract

The present invention relates to the field of sample detection, and provides a sample filling device, method and analyzer, which can preheat the sample through a preheater, and then maintain the temperature of the sample under low temperature conditions. At the same time, the sample is filtered through an air filter, and the reaction between substances in the air and the sample, which causes the sample to deteriorate, can be avoided, thereby ensuring the activity of the sample, making the detection of the sample faster and more accurate, and effectively avoiding the waste of the sample, and avoiding the problem of pipeline blockage caused by oxidation crystallization when the sample contacts the air.

Description

Technical Field

[0001] The present invention relates to the technical field of sample detection, and in particular to a sample filling device, method and analyzer. Background Art

[0002] When testing samples, the activity of the samples is often affected by temperature, air, etc. However, in the current sample filling system, there is a lack of a sample preheating device, and it is also impossible to avoid long-term contact between the sample and the air, which leads to reduced sample activity and thus affects the accuracy and speed of sample testing.

[0003] In addition, the existing sample filling method usually does not perform other operations after the sample testing is completed, causing the residual sample in the system to come into contact with the air during the standby process and oxidize and crystallize, which not only causes waste but also has the risk of pipeline blockage. Summary of the invention

[0004] In view of the above, it is necessary to provide a sample filling device, method and analyzer, aiming to solve the problems of low detection efficiency and low detection accuracy caused by affecting sample activity during the sample filling process.

[0005] A sample filling device, the sample filling device comprising:

[0006] at least one air filter for filtering air from a plurality of connected sample containers;

[0007] A plunger pump, used to absorb the filtered sample from a liquid inlet container selected from the plurality of sample containers through an opened liquid inlet valve, and then discharge the absorbed sample into a pipeline through an opened liquid outlet valve;

[0008] A preheater, used for preheating the sample in the pipeline;

[0009] Injection port, used to inject preheated sample into the analyzer.

[0010] According to a preferred embodiment of the present invention, each of the at least one air filter is connected in series with each sample container, and each air filter is used to absorb substances in the air that affect the activity of the sample.

[0011] According to a preferred embodiment of the present invention, the installation position of the preheater is close to the liquid injection port, and the preheater comprises:

[0012] A pipeline winding metal block, used for winding the pipeline;

[0013] A heating rod, installed in the middle of the metal block wound around the pipeline, for heating the sample in the pipeline;

[0014] A temperature sensor, installed at the outlet of the pipeline, for real-time monitoring of the temperature of the sample in the pipeline;

[0015] A temperature protection switch is connected in series with the heating rod, and is used to put the temperature protection switch into an off state and stop heating the sample in the pipeline when the temperature sensor detects that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, until the temperature sensor detects that the temperature of the sample in the pipeline is less than the temperature threshold, and continue to heat the sample in the pipeline by using the heating rod until the temperature sensor detects that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold;

[0016] The temperature protection switch is further used to, when the temperature sensor monitors that the temperature of the sample in the pipeline is lower than the temperature threshold, continue to heat the sample in the pipeline using the heating rod until the temperature sensor monitors that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold;

[0017] The heating rod is controlled by a PID algorithm to heat the sample in the pipeline.

[0018] According to a preferred embodiment of the present invention, the sample filling device further comprises:

[0019] The switching valve is used to select a sample container from the multiple sample containers as the liquid inlet container.

[0020] According to a preferred embodiment of the present invention, the plunger pump is further used to, when sucking the filtered sample from the liquid inlet container, immediately push back a second volume of sample after sucking a first volume of sample within a first preset time; wherein the first volume is greater than the second volume;

[0021] The plunger pump is also used to immediately absorb a fourth volume of sample after discharging a third volume of sample within a second preset time when discharging the absorbed sample into the pipeline through the opened liquid outlet valve; wherein the third volume is greater than the fourth volume.

[0022] According to a preferred embodiment of the present invention, the plunger pump is further used to determine the aspirated volume after detecting that the analyzer has completed the detection of the sample, and aspirate the sample of the aspirated volume through the opened liquid outlet valve;

[0023] The liquid outlet valve is also used to close the liquid outlet valve after the back suction is completed;

[0024] The liquid inlet valve is further used to open the liquid inlet valve after closing the liquid outlet valve;

[0025] The plunger pump is also used for resetting and discharging the sucked back sample into the liquid inlet container;

[0026] Wherein, the suction back volume includes the volume in the pipeline from the liquid outlet valve to the liquid injection port.

[0027] A sample filling method, the sample filling method comprising:

[0028] Air filtration for multiple connected sample containers;

[0029] aspirate the filtered sample from a liquid inlet container selected from the plurality of sample containers, and then discharge the aspirated sample into a pipeline;

[0030] preheating the sample in the pipeline;

[0031] Inject the preheated sample into the analyzer.

[0032] According to a preferred embodiment of the present invention, preheating the sample in the pipeline includes:

[0033] Heating the sample in the pipeline by using a PID algorithm;

[0034] monitoring the temperature of the sample in the pipeline in real time;

[0035] When it is monitored that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, the heating of the sample in the pipeline is stopped until it is monitored that the temperature of the sample in the pipeline is less than the temperature threshold, and the heating of the sample in the pipeline is continued until it is monitored that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold; or

[0036] When it is monitored that the temperature of the sample in the pipeline is lower than the temperature threshold, the sample in the pipeline continues to be heated until the temperature sensor monitors that the temperature of the sample in the pipeline reaches a configuration range of the temperature threshold.

[0037] According to a preferred embodiment of the present invention, the method further comprises:

[0038] After detecting that the analyzer has completed the detection of the sample, determining a back-sucking volume, and back-sucking the sample of the back-sucking volume;

[0039] The aspirated sample is discharged into the liquid inlet container.

[0040] An analyzer comprises the sample filling device, and is used for analyzing the sample after receiving the preheated sample injected by the sample filling device.

[0041] A computer device, comprising:

[0042] a memory storing at least one instruction; and

[0043] A processor executes instructions stored in the memory to implement the sample filling method.

[0044] A computer-readable storage medium stores at least one instruction, and the at least one instruction is executed by a processor in a computer device to implement the sample filling method.

[0045] It can be seen from the above technical scheme that the present invention can preheat the sample through the preheater, and thus maintain the temperature of the sample under low temperature conditions. At the same time, by filtering the sample through the air filter, it can also prevent the substances in the air from reacting with the sample and causing the sample to deteriorate, thereby ensuring the activity of the sample and making the sample detection faster and more accurate. At the same time, it effectively avoids the waste of the sample and avoids the problem of pipeline blockage caused by oxidation crystallization when the sample comes into contact with the air. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 Schematic diagram of the sample filling device of the present invention.

[0047] Figure 2 Schematic diagram of the substrate filling device of the present invention.

[0048] Figure 3 It is a cross-sectional view of the preheater in the present invention.

[0049] Figure 4 is a schematic diagram of the analyzer of the present invention.

[0050] Figure 5 It is a flow chart of a preferred embodiment of the sample injection method of the present invention.

[0051] Figure 6 It is a structural schematic diagram of a computer device of a preferred embodiment of the sample injection method of the present invention. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0053] like Figure 1 Shown is a schematic diagram of the sample filling device of the present invention.

[0054] The sample filling device 11 includes at least one air filter 110 for filtering air in a plurality of connected sample containers 115;

[0055] The plunger pump 112 is used to absorb the filtered sample from the liquid inlet container selected from the plurality of sample containers 115 through the opened liquid inlet valve, and then discharge the absorbed sample into the pipeline through the opened liquid outlet valve;

[0056] A preheater 113, used for preheating the sample in the pipeline;

[0057] The injection port 114 is used to inject the preheated sample into the analyzer.

[0058] The sample filling device 11 further includes: a switching valve 111 for selecting a sample container from the plurality of sample containers 115 as the liquid inlet container.

[0059] The sample may include, but is not limited to: substrate, pure water, cleaning solution, etc.

[0060] For example, when conducting chemiluminescent immunoassay, it is mainly divided into three categories according to the different markers: chemiluminescent immunoassay, chemiluminescent enzyme immunoassay, and electrochemiluminescent immunoassay. In chemiluminescent enzyme immunoassay, the luminescent system composed of alkaline phosphatase and 1,2-dioxetane is currently the most important and sensitive chemiluminescent system. 1,2-Dioxetane is a direct luminescent substrate for alkaline phosphatase, and its stability plays a decisive role in the accuracy and repeatability of the test results. In actual application, temperature will affect the luminescent value of the substrate background. Generally speaking, the substrate background value increases with increasing temperature. In addition, carbon dioxide in the air will reduce the pH value of the substrate and affect the activity of the enzyme. Long-term contact between the substrate and oxygen will lead to oxidative denaturation. However, the current sample addition method lacks substrate preheating, and it cannot avoid long-term contact between the substrate and air. In this embodiment, the substrate is preheated by a preheater, and at the same time, the substrate is filtered by an air filter, which ensures the activity of the substrate, makes the chemiluminescent immunoassay faster and more accurate, and effectively avoids the waste of the substrate and the pipeline blockage problem caused by oxidation crystallization when the substrate comes into contact with the air.

[0061] To give a further example, the air filter 110 can be an air filtering device for absorbing carbon dioxide and oxygen in the air; the switching valve 111 can be a three-way solenoid valve, which is mainly used in the online switching process of the substrate bottle; the plunger pump 112 can be a power device for substrate addition, which is used for absorbing and adding substrates; the preheater 113 can be a device for heating the substrate pipeline, thereby preheating the substrate solution in the pipeline; the inlet valve can be a substrate inlet valve, which is a two-way solenoid valve, which is mainly used in the substrate absorption process; the outlet valve can be a substrate outlet valve, which is a two-way solenoid valve, which is mainly used in the substrate addition process.

[0062] For example Figure 2 As shown, it is a schematic diagram of the substrate filling device of the present invention. Wherein, the substrate filling device is mainly composed of a liquid injection port 114, a plunger pump 112, a liquid inlet valve 211, a liquid outlet valve 212, a substrate switching valve 111, and a substrate preheater 113, a bubble sensor 215, two air filters 110 and related pipeline joints. Specifically, the plunger pump 112 provides power, and the liquid inlet valve 211 and the liquid outlet valve 212 are respectively installed at the inlet and outlet of the plunger pump 112 to achieve the suction and injection of the substrate. A three-way valve (i.e., the substrate switching valve 111) is used upstream, and the two selection inlets of the valve are respectively connected to two substrate bottles (i.e., the sample container 115), and the outlet is connected to the liquid inlet valve 211. When one bottle of substrate is exhausted, the other bottle can be replaced to ensure that the substrate liquid is replaced online without stopping the machine.

[0063] In this embodiment, each air filter 110 of the at least one air filter 110 is connected in series with each sample container 115 , respectively, and each air filter 110 is used to absorb substances in the air that affect the activity of the sample.

[0064] For example, each air filter 110 may contain ingredients such as soda lime and sodium sulfite, which can absorb carbon dioxide and oxygen in the air, thereby preventing carbon dioxide in the air from affecting the enzyme activity of the sample and oxygen from oxidizing the sample.

[0065] It is understandable that the container cover of the sample container 115 (such as a substrate bottle) usually adopts a sealed structure. In order to balance the pressure between the sample container 115 and the outside world, the sample container 115 needs to be connected to the outside world, but substances in the air usually react with the sample to cause the sample to deteriorate (such as carbon dioxide in the air will reduce the pH value of the substrate and affect the activity of the enzyme, and long-term contact between the substrate and oxygen will cause oxidation). Therefore, when the air enters the sample container 115, it needs to be filtered (such as each substrate bottle cap has a pipeline connected to the air filter).

[0066] In the above embodiment, by connecting each air filter 110 in series with each sample container 115 , the sample in each sample container 115 can be prevented from directly contacting the air, thereby preventing the sample from deteriorating due to reaction with substances in the air.

[0067] like Figure 3 , which is a cross-sectional view of the preheater in the present invention. The installation position of the preheater 113 is close to the liquid injection port 114, and the preheater 113 includes:

[0068] A pipeline winding metal block 1131, used for winding the pipeline;

[0069] A heating rod 1132 is installed in the middle of the pipeline-wrapped metal block 1131 and is used to heat the sample in the pipeline;

[0070] A temperature sensor 1133 is installed at the outlet of the pipeline and is used to monitor the temperature of the sample in the pipeline in real time;

[0071] The temperature protection switch 1134 is connected in series with the heating rod 1132, and is used to put the temperature protection switch 1134 into an off state and stop heating the sample in the pipeline when the temperature sensor 1133 detects that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, until the temperature sensor 1133 detects that the temperature of the sample in the pipeline is less than the temperature threshold, and continue to heat the sample in the pipeline by using the heating rod 1132 until the temperature sensor 1133 detects that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold;

[0072] The temperature protection switch 1134 is also used to continue heating the sample in the pipeline using the heating rod 1132 when the temperature sensor 1133 monitors that the temperature of the sample in the pipeline is lower than the temperature threshold, until the temperature sensor 1133 monitors that the temperature of the sample in the pipeline reaches the configured range of the temperature threshold.

[0073] Continuing with the above example, in the substrate filling equipment, the heating rod 1132 can be a DC heater, which can generate heat when powered on and is the heat source of the substrate preheater 113; the temperature sensor 1133 can be an electronic component for monitoring temperature, used to monitor the temperature of the substrate preheater 113; the temperature protection switch 1134 can be a temperature protection component to prevent overheating caused by temperature control out of control; the pipeline winding metal block 1131 can be an aluminum metal block, which is the main device of the substrate preheater 113 and is mainly used for winding the substrate pipeline.

[0074] Specifically, the preheater 113 further includes a metal sleeve 1135 and a circumferential heat-insulating foam 1136. The pipeline is wound on the pipeline-wound metal block 1131, the heating rod 1132 is used in series with the temperature protection switch 1134, the temperature sensor 1133 senses the temperature, and controls the output of the heating rod 1132 through a combination of software and hardware, thereby heating the pipeline-wound metal block 1131, and finally indirectly heating the sample in the pipeline.

[0075] Among them, after the sample comes out of the preheater 113, it has to pass through a section of pipeline, and this section of pipeline is exposed to the air. Therefore, after the sample comes out of the preheater 113 and passes through this section of pipeline, the temperature is likely to drop slightly, and the shorter the section of pipeline is, the less the temperature drops.

[0076] Therefore, during the installation process, the installation position of the preheater 113 needs to be as close to the liquid injection port 114 as possible.

[0077] Among them, winding the pipeline around the pipeline winding metal block 1131 in a winding manner not only makes the heating more uniform, but also adopts the winding method to make the volume of the preheater 113 smaller and save more space.

[0078] The heating rod 1132 is connected in series with the temperature protection switch 1134 to achieve temperature protection. Specifically, when the system is out of control and the temperature is too high, the temperature protection switch 1134 will be disconnected, and the heating rod 1132 connected in series with it will also be powered off.

[0079] The samples coming out of the preheater 113 must pass through the outlet of the preheater 113 . The temperature sensor 1133 is installed at the outlet of the pipeline to ensure the consistency of the temperature of the samples passing through the outlet.

[0080] Specifically, when reading the temperature value of the temperature sensor 1133, one decimal place can be retained, that is, accurate to 0.1°C, and then read in real time.

[0081] The temperature threshold refers to the target heating temperature of the preheater 113. When the preheater 113 reaches the target heating temperature, the entire preheater 113 (including the wound pipeline) will become a constant temperature body. At this time, the temperature threshold is the temperature of the sample in the pipeline.

[0082] The configuration range may be custom configured, such as within the range of ±0.3°C of the temperature threshold.

[0083] In the above embodiment, the preheater 113 can be used to preheat the sample, thereby maintaining the temperature of the sample under low temperature conditions, avoiding the influence of temperature on the sample, and maintaining the activity of the sample.

[0084] Specifically, the preheater 113 controls the heating rod 1132 to heat the sample in the pipeline through a PID (Proportional Integral Derivative) algorithm.

[0085] Among them, the PID algorithm is a relatively mature control algorithm and will not be elaborated here.

[0086] In this embodiment, the plunger pump 112 is also used to push back the second volume of sample immediately after sucking the first volume of sample within a first preset time when sucking the filtered sample from the liquid inlet container;

[0087] Wherein, the first volume is greater than the second volume.

[0088] The first preset time can be configured according to actual needs.

[0089] Continuing with the above example, in order to finally complete the filling of 200ul substrate, the substrate inlet valve 211 is opened, and the substrate plunger pump 112 begins to reset under the cycle instruction. The substrate plunger pump 112 completes the first volume of sample aspiration 240ul within the first preset time, and immediately pushes back the second volume of sample 20ul.

[0090] In this embodiment, the plunger pump 112 is further used to immediately absorb a fourth volume of sample after discharging a third volume of sample within a second preset time when discharging the absorbed sample into the pipeline through the opened liquid outlet valve 212;

[0091] Wherein, the third volume is greater than the fourth volume.

[0092] The second preset time can be configured according to actual needs.

[0093] Continuing with the above example, the substrate inlet valve 211 is closed, the substrate outlet valve 212 is opened, and the substrate plunger pump 112 completes the discharge of the third volume of 210ul substrate within the second preset time. After the substrate is discharged, in order to prevent the substrate from hanging liquid, the fourth volume of 10ul substrate suction action is immediately completed. After the sample is discharged, the substrate outlet valve 212 is closed.

[0094] In the above embodiment, after the third volume of sample is discharged within the second preset time, the fourth volume of sample is immediately drawn, which can prevent liquid hanging, that is, prevent liquid hanging at the outlet of the substrate liquid outlet tube. If liquid hanging occurs, the most direct impact is that the accuracy of substrate sample addition will be reduced and the repeatability will be increased, which will eventually affect the detection results.

[0095] It should be noted that the numbers such as 240ul, 20ul, 210ul, 10ul, etc. involved in the filling process are intermediate process quantities obtained based on calculations and actual experiments.

[0096] In this embodiment, the plunger pump 112 is also used to determine the aspirated volume after detecting that the analyzer has completed the detection of the sample, and aspirate the sample of the aspirated volume through the opened liquid outlet valve 212;

[0097] The liquid outlet valve 212 is also used to close the liquid outlet valve 212 after the back suction is completed;

[0098] The liquid inlet valve 211 is also used to open the liquid inlet valve 211 after closing the liquid outlet valve 212;

[0099] The plunger pump 112 is also used for resetting and discharging the sucked back sample into the liquid inlet container;

[0100] The back-sucking volume includes the volume in the pipeline from the liquid outlet valve 212 to the liquid injection port 114 .

[0101] After detecting that the analyzer has completed the detection of the sample, the analyzer enters a standby state and no more sample addition is performed.

[0102] Continuing with the above example, after detecting that the analyzer has completed the detection of the substrate, the substrate liquid outlet valve 212 is opened, and the substrate plunger pump 112 sucks back 490ul (in the pipeline, the volume from the substrate liquid outlet valve 212 to the substrate injection port 114 is 490ul, therefore, configuring the sucking back volume to be 490ul can ensure that all the substrate is sucked back into the liquid outlet valve 212). After the sucking back is completed, the substrate liquid outlet valve 212 is closed, the substrate liquid inlet valve 211 is opened, and finally the substrate plunger pump 112 is reset to discharge the substrate into the substrate bottle (i.e., the sample container 115).

[0103] Through the above-mentioned implementation, after each test is completed, a sample aspiration is performed to aspirate the sample in the pipeline back into the liquid outlet valve 212, so that the sample at the pipeline outlet can be isolated from the air, thereby avoiding long-term contact between the sample at the pipeline outlet and the air during the standby process after the test, thereby reducing the risk of sample oxidation crystallization.

[0104] When it is detected that the analyzer has not completed the detection of the sample, the sampling and adding process is continued, which will not be described in detail here.

[0105] It can be seen from the above technical scheme that the present invention can preheat the sample through the preheater, and thus maintain the temperature of the sample under low temperature conditions. At the same time, by filtering the sample through the air filter, it can also prevent the substances in the air from reacting with the sample and causing the sample to deteriorate, thereby ensuring the activity of the sample and making the sample detection faster and more accurate. At the same time, it effectively avoids the waste of the sample and avoids the problem of pipeline blockage caused by oxidation crystallization when the sample comes into contact with the air.

[0106] like Figure 4 Shown is a schematic diagram of the analyzer of the present invention.

[0107] The analyzer 44 is used to analyze the sample after receiving the preheated sample injected by the sample filling device. The analyzer 44 includes:

[0108] A sample tray 441 is used to provide samples to be tested;

[0109] Reagent disk 442, used to provide reagents required to participate in the reaction;

[0110] The reaction cup automatic sorting device 443 is used to provide reaction cups;

[0111] The incubation tray 444 is used to provide a place for the sample and the reagent to react in the reaction cup;

[0112] Magnetic separation disk 445, including Figure 1 The sample filling device 11;

[0113] The detection device 446 is used to detect the liquid after magnetic separation.

[0114] like Figure 5 FIG. 1 is a flow chart of a preferred embodiment of the sample injection method of the present invention. According to different requirements, the order of the steps in the flow chart can be changed, and some steps can be omitted.

[0115] The sample injection method is applied to one or more computer devices, which are devices that can automatically perform numerical calculations and / or information processing according to pre-set or stored instructions, and their hardware includes but is not limited to microprocessors, application specific integrated circuits (ASIC), programmable gate arrays (FPGA), digital signal processors (DSP), embedded devices, etc.

[0116] The computer device may be any electronic product that can perform human-computer interaction with a user, such as a personal computer, a tablet computer, a smart phone, a personal digital assistant (PDA), a game console, an interactive network television (IPTV), a smart wearable device, etc.

[0117] The computer device may also include a network device and / or a user device, wherein the network device includes, but is not limited to, a single network server, a server group consisting of multiple network servers, or a cloud consisting of a large number of hosts or network servers based on cloud computing.

[0118] The server can be an independent server or a cloud server that provides basic cloud computing services such as cloud services, cloud databases, cloud computing, cloud functions, cloud storage, network services, cloud communications, middleware services, domain name services, security services, content delivery networks (CDN), as well as big data and artificial intelligence platforms.

[0119] Among them, Artificial Intelligence (AI) is the theory, method, technology and application system that uses digital computers or machines controlled by digital computers to simulate, extend and expand human intelligence, perceive the environment, acquire knowledge and use knowledge to obtain the best results.

[0120] AI basic technologies generally include sensors, dedicated AI chips, cloud computing, distributed storage, big data processing technology, operation / interaction systems, mechatronics, etc. AI software technologies mainly include computer vision technology, robotics technology, biometrics technology, speech processing technology, natural language processing technology, and machine learning / deep learning.

[0121] The network where the computer device is located includes but is not limited to the Internet, a wide area network, a metropolitan area network, a local area network, a virtual private network (VPN), etc.

[0122] S10, performing air filtration on the multiple connected sample containers.

[0123] Specifically, an air filter capable of absorbing substances in the air that affect the activity of the sample may be used to filter the air of the connected multiple sample containers.

[0124] Through the above implementation, it is possible to prevent the sample in each sample container from directly contacting the air, thereby preventing the sample from deteriorating due to reaction with substances in the air.

[0125] S11, aspirating the filtered sample from a liquid inlet container selected from the plurality of sample containers, and then discharging the aspirated sample into a pipeline.

[0126] In this embodiment, a sample container containing sufficient sample can be preferentially selected as the liquid inlet container.

[0127] The pipeline refers to the pipeline for sample transmission.

[0128] S12, preheating the sample in the pipeline.

[0129] In this embodiment, preheating the sample in the pipeline includes:

[0130] Heating the sample in the pipeline by using a PID (Proportional Integral Derivative) algorithm;

[0131] monitoring the temperature of the sample in the pipeline in real time;

[0132] When it is monitored that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, the heating of the sample in the pipeline is stopped until it is monitored that the temperature of the sample in the pipeline is less than the temperature threshold, and the heating of the sample in the pipeline is continued until it is monitored that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold; or

[0133] When it is monitored that the temperature of the sample in the pipeline is lower than the temperature threshold, the sample in the pipeline continues to be heated until the temperature sensor monitors that the temperature of the sample in the pipeline reaches a configuration range of the temperature threshold.

[0134] The temperature threshold refers to the target heating temperature. When the target heating temperature is reached, the entire preheater (including the wound pipeline) will become a constant temperature body. At this time, the temperature threshold is the temperature of the sample in the pipeline.

[0135] The configuration range may be custom configured, such as within the range of ±0.3°C of the temperature threshold.

[0136] In the above embodiment, the sample can be preheated by the preheater, and the temperature of the sample can be maintained even under low temperature conditions, thereby avoiding the influence of temperature on the sample and maintaining the activity of the sample.

[0137] S13, injecting the preheated sample into the analyzer.

[0138] In this embodiment, after the preheated sample is injected into the analyzer, the analyzer can be used to perform sample detection.

[0139] In this embodiment, the method further includes:

[0140] After detecting that the analyzer has completed the detection of the sample, determining a back-sucking volume, and back-sucking the sample of the back-sucking volume;

[0141] The aspirated sample is discharged into the liquid inlet container.

[0142] Through the above implementation, after each test is completed, a sample aspiration is performed to aspirate the sample in the pipeline back into the liquid outlet valve, which can isolate the sample at the pipeline outlet from the air, thereby avoiding long-term contact between the sample at the pipeline outlet and the air during the standby process after the test, thereby reducing the risk of sample oxidation and crystallization.

[0143] When it is detected that the analyzer has not completed the detection of the sample, the sampling and adding process is continued, which will not be described in detail here.

[0144] It can be seen from the above technical scheme that the present invention can preheat the sample through the preheater, and thus maintain the temperature of the sample under low temperature conditions. At the same time, by filtering the sample through the air filter, it can also prevent the substances in the air from reacting with the sample and causing the sample to deteriorate, thereby ensuring the activity of the sample and making the sample detection faster and more accurate. At the same time, it effectively avoids the waste of the sample and avoids the problem of pipeline blockage caused by oxidation crystallization when the sample comes into contact with the air.

[0145] like Figure 6 FIG. 1 is a schematic diagram of the structure of a computer device of a preferred embodiment of the sample filling method of the present invention.

[0146] The computer device 500 is a server, which can be an independent server or a server cluster composed of multiple servers. The server can be an independent server or a cloud server that provides basic cloud computing services such as cloud services, cloud databases, cloud computing, cloud functions, cloud storage, network services, cloud communications, middleware services, domain name services, security services, content delivery networks (CDNs), and big data and artificial intelligence platforms.

[0147] Among them, Artificial Intelligence (AI) is the theory, method, technology and application system that uses digital computers or machines controlled by digital computers to simulate, extend and expand human intelligence, perceive the environment, acquire knowledge and use knowledge to obtain the best results.

[0148] AI basic technologies generally include sensors, dedicated AI chips, cloud computing, distributed storage, big data processing technology, operation / interaction systems, mechatronics, etc. AI software technologies mainly include computer vision technology, robotics technology, biometrics technology, speech processing technology, natural language processing technology, and machine learning / deep learning.

[0149] See also Figure 6The computer device 500 includes a processor 502 , a memory and a network interface 505 connected via a system bus 501 , wherein the memory may include a storage medium 503 and an internal memory 504 .

[0150] The storage medium 503 may store an operating system 5031 and a computer program 5032. When the computer program 5032 is executed, the processor 502 may execute a sample injection method.

[0151] The processor 502 is used to provide computing and control capabilities to support the operation of the entire computer device 500 .

[0152] The internal memory 504 provides an environment for the operation of the computer program 5032 in the storage medium 503. When the computer program 5032 is executed by the processor 502, the processor 502 can execute the sample filling method.

[0153] The network interface 505 is used for network communication, such as providing data information transmission, etc. Those skilled in the art will understand that Figure 6 The structure shown in the figure is only a block diagram of a part of the structure related to the solution of the present invention, and does not constitute a limitation on the computer device 500 to which the solution of the present invention is applied. The specific computer device 500 may include more or fewer components than those shown in the figure, or combine certain components, or have a different arrangement of components.

[0154] The processor 502 is used to run a computer program 5032 stored in the memory to implement the sample filling method disclosed in the embodiment of the present invention.

[0155] Those skilled in the art will understand that Figure 6 The embodiments of the computer device shown in the figure do not constitute a limitation on the specific composition of the computer device. In other embodiments, the computer device may include more or fewer components than shown in the figure, or combine certain components, or arrange the components differently. For example, in some embodiments, the computer device may only include a memory and a processor. In such embodiments, the structure and function of the memory and the processor are the same as those of the embodiment of the present invention. Figure 6 The embodiments shown are consistent and will not be described again here.

[0156] It should be understood that in the embodiment of the present invention, the processor 502 may be a central processing unit (CPU), and the processor 502 may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. Among them, the general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0157] In another embodiment of the present invention, a computer-readable storage medium is provided. The computer-readable storage medium may be a non-volatile computer-readable storage medium or a volatile computer-readable storage medium. The computer-readable storage medium stores a computer program, wherein when the computer program is executed by a processor, the sample injection method disclosed in the embodiment of the present invention is implemented.

[0158] It should be noted that the data involved in this case were all obtained legally.

[0159] Those skilled in the art can clearly understand that, for the convenience and simplicity of description, the specific working process of the above-described equipment, devices and units can refer to the corresponding process in the aforementioned method embodiment, and will not be repeated here. Those of ordinary skill in the art can appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented with electronic hardware, computer software or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in the above description according to the function. Whether these functions are executed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to exceed the scope of the present invention.

[0160] In the several embodiments provided by the present invention, it should be understood that the disclosed equipment, devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation. Units with the same function may also be combined into one unit. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. In addition, the mutual coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection through some interfaces, devices or units, or may be an electrical, mechanical or other form of connection.

[0161] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiments of the present invention.

[0162] The present invention can be used in many general or special computer system environments or configurations. For example: personal computers, server computers, handheld or portable devices, tablet devices, multiprocessor systems, microprocessor-based systems, set-top boxes, programmable consumer electronic devices, network PCs, minicomputers, mainframe computers, distributed computing environments including any of the above systems or devices, etc. The present invention can be described in the general context of computer-executable instructions executed by a computer, such as program modules. Generally, program modules include routines, programs, objects, components, data structures, etc. that perform specific tasks or implement specific abstract data types. The present invention can also be practiced in distributed computing environments, in which tasks are performed by remote processing devices connected through a communication network. In a distributed computing environment, program modules can be located in local and remote computer storage media including storage devices.

[0163] In addition, each functional unit in each embodiment of the present invention may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.

[0164] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a storage medium. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a storage medium, including several instructions for a computer device (which can be a personal computer, server, or network device, etc.) to perform all or part of the steps of the method described in each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), disk or optical disk and other media that can store program code.

[0165] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A sample filling device, characterized in that: The sample filling device comprises: at least one air filter for filtering air from a plurality of connected sample containers; A plunger pump, used to absorb the filtered sample from the liquid inlet container selected from the plurality of sample containers through the opened liquid inlet valve, and then discharge the absorbed sample into the pipeline through the opened liquid outlet valve; wherein the sample includes a substrate or pure water or a cleaning solution; A preheater, used for preheating the sample in the pipeline; A liquid injection port, used to inject the preheated sample into the analyzer; The plunger pump is further used to push back a second volume of sample immediately after absorbing a first volume of sample within a first preset time when absorbing the filtered sample from the liquid inlet container; wherein the first volume is greater than the second volume; The plunger pump is also used to immediately absorb a fourth volume of sample after discharging a third volume of sample within a second preset time when discharging the absorbed sample into the pipeline through the opened liquid outlet valve; wherein the third volume is greater than the fourth volume.

2. The sample filling device according to claim 1, characterized in that: Each of the at least one air filter is connected in series with a sample container, and each air filter is used to absorb substances in the air that affect the activity of the sample.

3. The sample filling device according to claim 1, characterized in that: The installation position of the preheater is close to the liquid injection port, and the preheater comprises: A pipeline winding metal block, used for the pipeline to be wound; A heating rod, installed in the middle of the metal block wound around the pipeline, for heating the sample in the pipeline; A temperature sensor, installed at the outlet of the pipeline, for real-time monitoring of the temperature of the sample in the pipeline; A temperature protection switch is connected in series with the heating rod, and is used to put the temperature protection switch into an off state and stop heating the sample in the pipeline when the temperature sensor detects that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, until the temperature sensor detects that the temperature of the sample in the pipeline is less than the temperature threshold, and continue to heat the sample in the pipeline by using the heating rod until the temperature sensor detects that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold; The temperature protection switch is further used to, when the temperature sensor monitors that the temperature of the sample in the pipeline is lower than the temperature threshold, continue to heat the sample in the pipeline using the heating rod until the temperature sensor monitors that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold; The heating rod is controlled by a PID algorithm to heat the sample in the pipeline.

4. The sample filling device according to any one of claims 1 to 3, characterized in that: The sample filling device also includes: The switching valve is used to select a sample container from the multiple sample containers as the liquid inlet container.

5. The sample filling device according to any one of claims 1 to 3, characterized in that: The plunger pump is further used to determine the aspirated volume after detecting that the analyzer has completed the detection of the sample, and aspirate the sample of the aspirated volume through the opened liquid outlet valve; The liquid outlet valve is also used to close the liquid outlet valve after the back suction is completed; The liquid inlet valve is further used to open the liquid inlet valve after closing the liquid outlet valve; The plunger pump is also used for resetting and discharging the sucked back sample into the liquid inlet container; Wherein, the suction back volume includes the volume in the pipeline from the liquid outlet valve to the liquid injection port.

6. A sample filling method, characterized in that: The sample filling method comprises: Air filtration for multiple connected sample containers; Aspirating the filtered sample from a liquid inlet container selected from the plurality of sample containers, and then discharging the aspirated sample into a pipeline; wherein the sample includes a substrate or pure water or a cleaning solution; preheating the sample in the pipeline; Inject the preheated sample into the analyzer; When the filtered sample is sucked from the liquid inlet container, after sucking a first volume of the sample within a first preset time, a second volume of the sample is immediately pushed back; wherein the first volume is greater than the second volume; When the sucked sample is discharged into the pipeline through the opened liquid outlet valve, after the third volume of the sample is discharged within the second preset time, the fourth volume of the sample is immediately sucked; wherein the third volume is greater than the fourth volume.

7. The sample injection method according to claim 6, characterized in that: The preheating of the sample in the pipeline comprises: Heating the sample in the pipeline by using a PID algorithm; monitoring the temperature of the sample in the pipeline in real time; When it is monitored that the temperature of the sample in the pipeline is greater than or equal to the temperature threshold, the heating of the sample in the pipeline is stopped until it is monitored that the temperature of the sample in the pipeline is less than the temperature threshold, and the heating of the sample in the pipeline is continued until it is monitored that the temperature of the sample in the pipeline reaches the configuration range of the temperature threshold; or When it is monitored that the temperature of the sample in the pipeline is lower than the temperature threshold, the sample in the pipeline continues to be heated until the temperature sensor monitors that the temperature of the sample in the pipeline reaches a configuration range of the temperature threshold.

8. The sample filling method according to claim 6 or 7, characterized in that: The method further comprises: After detecting that the analyzer has completed the detection of the sample, determining a back-sucking volume, and back-sucking the sample of the back-sucking volume; The aspirated sample is discharged into the liquid inlet container.

9. An analyzer, characterized in that: The analyzer comprises the sample filling device according to any one of claims 1 to 5, and the analyzer is used to analyze the sample after receiving the preheated sample injected by the sample filling device.

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