A new type of automated immunohistochemical staining instrument

Abstract

The present invention relates to a novel automated immunohistochemical staining instrument, which comprises a workbench, an automatic circle drawing component and a liquid adding tank component, wherein the automatic circle drawing component and the liquid adding tank component are both arranged on the workbench. The automatic circle drawing component comprises a slide module, a crossbeam and a mounting block for mounting a dripping module, wherein the liquid outlet of the dripping module faces the workbench, the crossbeam is fixedly mounted on the workbench, the mounting block is slidably mounted on the crossbeam, the slide module is used to accommodate a slide rack, a plurality of stations for placing slides are arranged on the slide rack, the slide module is slidably mounted on the workbench, the slide module can move simultaneously in the X direction and the Y direction, the X direction is perpendicular to the Y direction, and the slide module is located between the workbench and the mounting block. The liquid adding tank component comprises a liquid adding chamber and a liquid adding rack arranged on the workbench, the liquid adding chamber is used to place the slide rack, a plurality of liquid adding needles are arranged on the liquid adding rack, the outlet of the liquid adding needle is located above the slide rack, and the inlet of the liquid adding needle is connected to a medicine bottle.

Description

Technical Field

[0001] The invention relates to the field of medical devices, and in particular to a novel automated immunohistochemical staining instrument. Background Art

[0002] The fully automatic immunohistochemical staining instrument is an immunohistochemical staining system that can complete the entire process from dewaxing to staining. It has been widely used in hospitals. However, most of the models available on the market require a cover plate as an accessory consumable to work when performing liquid reactions. Moreover, this cover plate often has a limited number of uses. As the number of operations increases, a lot of discarded covers will be generated, making subsequent processing more difficult. Some models use the immersion method in the reagent tank during the reaction stage, which will generate a large amount of waste liquid and increase the reagent cost of a single experiment. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] The invention provides a novel automatic immunohistochemical staining instrument, aiming to solve the technical problem that too many waste covers and waste liquids are generated during the use of the immunohistochemical staining instrument in the prior art.

[0005] (II) Technical solution

[0006] In order to solve the above problems, the present invention provides a novel automated immunohistochemical staining instrument, which comprises: a workbench, an automatic circle drawing component, a liquid transfer component and a liquid adding tank component, wherein the automatic circle drawing component, the liquid transfer needle component and the liquid adding tank component are all arranged on the workbench;

[0007] The automatic circle drawing assembly includes a slide module, a crossbeam and a mounting block for mounting a dripping module, the liquid outlet of the dripping module faces the workbench, the crossbeam is fixedly mounted on the workbench, the mounting block is slidably mounted on the crossbeam, the slide module is used to accommodate a glass slide rack, the glass slide rack is provided with a plurality of stations for placing glass slides, the slide module is slidably mounted on the workbench, the slide module can move in the X direction and the Y direction at the same time, the X direction is perpendicular to the Y direction, and the slide module is located between the workbench and the mounting block;

[0008] The pipetting assembly comprises a reagent bottle fixing frame, a pipetting needle, a pipetting sliding module and a pipetting fixing frame, wherein the reagent bottle fixing frame is slidably mounted on the workbench, the reagent bottle fixing frame can move along the Y direction on the workbench, and a plurality of cavities for placing reagent bottles are arranged on the reagent bottle fixing frame, the pipetting fixing frame is fixedly mounted on the workbench, the pipetting sliding module is slidably mounted on the pipetting fixing frame, the pipetting needle is fixedly mounted on the pipetting sliding module, the pipetting needle can move along the X direction and the Z direction at the same time, the Z direction is perpendicular to the X direction and the Y direction respectively, and the pipetting needle can absorb the reagent in the reagent bottle and drop the reagent on the carrier sheet;

[0009] The liquid adding tank assembly includes a liquid adding cavity and a liquid adding rack arranged on the workbench, the liquid adding cavity is used to place the glass slide rack, and the liquid adding rack is provided with a plurality of liquid adding needles, the outlets of the liquid adding needles are located above the glass slide rack, and the inlets of the liquid adding needles are connected to the medicine bottles.

[0010] Preferably, the novel automated immunohistochemical staining instrument further comprises a baking component, the baking component comprises a baking box fixedly mounted on the workbench, the baking box comprises a baking cavity and an air guide cavity, a first side of the baking cavity is provided with a plurality of strip-shaped notches, a second side and a third side of the baking cavity connected to the first side are respectively provided with baking installation strips corresponding to the strip-shaped notches, the slide rack can enter the baking cavity along the strip-shaped notches and be placed between the two baking installation strips, and a sliding door for controlling the opening and closing of the baking cavity is slidably provided on a side of the baking cavity opposite to the first side;

[0011] The air guide cavity is connected to and communicates with the slice baking cavity. A heating module and a heat-conducting fan are arranged in the air guide cavity. The heat-conducting fan can blow hot air into the slice baking cavity.

[0012] Preferably, the novel automated immunohistochemistry staining instrument further comprises an incubation component, which comprises a plurality of incubation chambers stacked on the workbench, wherein the incubation chambers are all located below the pipetting fixture, a heating device is arranged on the outer surface of the bottom plate of the incubation chamber, a plurality of heat dissipation strips are arranged at intervals on the inner surface of the bottom plate of the incubation chamber, and an incubation plate for placing the slide rack is arranged in the incubation chamber.

[0013] Preferably, the incubation chamber is provided with a first opening, and an ejection motor is arranged on the side panel of the incubation chamber opposite to the first opening, the output shaft of the ejection motor is connected to the first screw rod, the first screw rod faces the first opening, and the axial direction of the first screw rod is the same as the Y direction, and an internal threaded hole matching the first screw rod is arranged on the incubation plate.

[0014] Preferably, the novel automated immunohistochemical staining instrument further comprises a transport component, and the transport component comprises a transport rack, a transport sliding module and a mechanical gripper;

[0015] The transfer rack is fixedly arranged on the workbench, the mechanical gripper is fixedly installed on the transfer sliding module, the transfer sliding module is slidably installed on the transfer rack, and the transfer sliding module can move along the X direction, the Y direction and the Z direction at the same time;

[0016] The mechanical gripper includes a gripper fixing frame and a pair of clamping rods arranged on the gripper fixing frame, the gripper fixing frame is fixedly installed on the transfer sliding module, the clamping rods are slidably arranged on the gripper fixing frame, and the two clamping rods can clamp the glass slide rack, and the mechanical gripper can clamp the glass slide rack through the clamping rods and transport the glass slide rack to any one of the slide board module, the liquid adding chamber, the baking chamber and the incubation plate.

[0017] Preferably, the transfer sliding module comprises a transfer horizontal plate, a transfer vertical plate and a transfer sliding block;

[0018] The transfer transverse plate is slidably installed on the transfer frame, and the transfer transverse plate can move along the X direction on the transfer frame. The transfer vertical plate is slidably installed on the transfer transverse plate, and the transfer vertical plate can move along the Y direction on the transfer transverse plate. The transfer slider is slidably installed on the transfer vertical plate, and the transfer slider can move along the Z direction on the transfer vertical plate. The gripper fixing frame is fixedly installed on the transfer slider.

[0019] Preferably, the slide module comprises a first slide and a second slide, and the top surface of the second slide is slidably connected to the bottom surface of the first slide;

[0020] An X-guide rail and an X-axis driving module are arranged on the side of the first slide plate, an X-sliding block slidably connected to the X-guide rail is arranged on the workbench, the direction of the X-guide rail is consistent with the X direction, and the X-driving assembly can drive the X-guide rail to move along the X direction under the guidance of the X-sliding block;

[0021] A Y guide rail and a Y-axis driving module are arranged on the bottom surface of the first slide plate, the direction of the Y guide rail is consistent with the Y direction, a Y slider slidably connected to the Y guide rail is fixedly arranged on the second slide plate, and the Y-axis driving module can drive the Y slider to move along the Y direction;

[0022] The first slide plate is provided with a working through hole penetrating the bottom surface and the top surface of the first slide plate, the cross section of the working through hole is rectangular, and the glass slide rack is placed on the second slide plate through the working through hole;

[0023] The bottom surface of the first slide plate is also provided with a dovetail groove, the width of the dovetail groove is smaller than the width of the groove bottom, and the top surface of the second slide plate is also provided with a slide rail matching the dovetail groove, and the directions of the dovetail groove and the slide rail are consistent with the Y direction.

[0024] Preferably, the X-axis drive module comprises a first motor, a first fixed plate and an X-axis screw rod, the first slide plate is provided with an internal threaded hole matched with the X-axis screw rod; the first fixed plate is fixedly mounted on the workbench, the first motor is fixedly mounted on one side of the first fixed plate, one end of the X-axis screw rod is rotatably connected to the output shaft of the first motor, the other end of the X-axis screw rod is mounted in the internal threaded hole on the first slide plate, and the direction of the X-axis screw rod is consistent with the X direction;

[0025] The Y-axis driving module includes a second motor, a second fixed plate and a Y-axis screw rod. The Y slider is provided with an internal threaded hole that cooperates with the Y-axis screw rod. One end of the second fixed plate is fixedly mounted on the bottom surface of the first slider, and the second motor is fixedly mounted on one side of the second fixed plate. One end of the Y-axis screw rod is rotatably connected to the output shaft of the second motor, and the other end of the Y-axis screw rod is mounted in the internal threaded hole of the Y slider. The direction of the Y-axis screw rod is consistent with the Y direction.

[0026] Preferably, the pipetting sliding module includes a pipetting transverse plate, a pipetting vertical rod and a pipetting sliding block, the pipetting fixed frame is fixedly installed on the workbench, the pipetting transverse plate is slidably arranged on the pipetting fixed frame, the pipetting transverse plate can move along the X direction on the pipetting fixed frame, the pipetting vertical rod is fixedly arranged on the pipetting transverse plate along the Z direction, the pipetting sliding block is slidably arranged on the pipetting vertical rod, and the pipetting needle is fixedly installed on the pipetting sliding block.

[0027] (III) Beneficial effects

[0028] The present invention drives the slide glass to move on the workbench in the X and Y directions at the same time through the slide module, so that the dripping module can draw a closed circle on the slide glass, so that when the reagent is subsequently dripped, the reagent only needs to be dripped in the circle, avoiding the use of the cover plate, thereby reducing the generation of discarded cover plates. At the same time, the present invention also places the slide rack in the liquid adding chamber, and drips the agent to the single slide glass through the liquid adding target to achieve the cleaning effect. When the slide glass is used in small batches, a large amount of waste liquid is avoided due to immersion, thereby reducing the reagent cost of a single experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the overall structure of a novel automated immunohistochemical staining instrument of the present invention;

[0030] Figure 2 for Figure 1 A magnified view at point A;

[0031] Figure 3 It is a structural schematic diagram of another perspective of a novel automated immunohistochemical staining instrument of the present invention;

[0032] Figure 4 It is a structural schematic diagram of the automatic circle drawing component in the present invention;

[0033] Figure 5 A schematic structural diagram of the automatic circle drawing component of the present invention from another perspective;

[0034] Figure 6 A cross-sectional view of the sliding connection between the first slide plate and the second slide plate in the present invention;

[0035] Figure 7 It is a structural schematic diagram of the baking sheet assembly in the present invention;

[0036] Figure 8 It is a structural schematic diagram of the grilling sheet assembly of the present invention from another perspective;

[0037] Fig. 9 It is a schematic diagram of the structure of the incubation chamber in the present invention;

[0038] Fig.10 This is a schematic structural diagram of the incubation chamber in the present invention from another perspective;

[0039] Fig.11 It is a schematic diagram of the structure of the transfer component in the present invention.

[0040] [Description of Reference Numerals]

[0041] 1: working table; 10: X slider;

[0042] 2: Automatic circle drawing component; 21: Slide board module; 210: First slide board; 2101: X guide rail; 2102: X axis driving module; 21021: First motor; 21022: First fixed plate; 21023: X axis screw rod; 2103: Y guide rail; 2104: Y axis driving module; 21041: Second motor; 21042: Second fixed plate; 21043: Y axis screw rod; 2105: Working through hole; 2106: Dovetail groove; 211: Second slide board; 2111: Y slider; 2112: Slide rail; 22: Crossbeam; 23: Drip module; 24: Mounting block;

[0043] 3: pipetting assembly; 31: reagent bottle fixing frame; 32: pipetting needle; 33: pipetting slide module; 331: pipetting horizontal plate; 332: pipetting vertical rod; 333: pipetting slide; 34: pipetting fixing frame;

[0044] 4: liquid adding tank assembly; 41: liquid adding chamber; 42: liquid adding rack; 43: liquid adding needle;

[0045] 5: baking sheet assembly; 50: baking sheet box; 51: baking sheet cavity; 511: first side surface; 512: strip-shaped notch; 513: baking sheet mounting strip; 514: sliding door; 52: air guide cavity;

[0046] 6: incubation assembly; 60: incubation chamber; 61: bottom plate; 611: heating device; 612: heat dissipation strip; 62: incubation plate; 63: first opening; 64: translation motor; 65: first screw rod;

[0047] 7: transfer assembly; 71: transfer rack; 72: transfer sliding module; 721: transfer horizontal plate; 722: transfer vertical plate; 723: transfer slide block; 73: mechanical gripper; 731: gripper fixing frame; 732: clamping rod.

[0048] 8: Slide rack. DETAILED DESCRIPTION

[0049] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below through specific implementation modes in conjunction with the accompanying drawings.

[0050] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0051] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0052] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0053] The present invention provides a novel automated immunohistochemical staining instrument, such as Figure 1 As shown, it comprises: a workbench 1, an automatic circle drawing component 2, a pipetting component 3 and a liquid adding tank component 4, wherein the automatic circle drawing component 2, the pipetting needle 32 component and the liquid adding tank component 4 are all arranged on the workbench 1.

[0054] The automatic circle drawing component 2 includes a slide module 21, a beam 22 and a mounting block 24 for mounting a drip module 23. The liquid outlet of the drip module 23 faces the workbench 1. The drip module 23 stores working liquid, and the working liquid can flow out from the liquid outlet of the drip module. The beam 22 is fixedly installed on the workbench 1, and the mounting block 24 is slidably installed on the beam 22. The slide module 21 is used to accommodate a glass slide rack 8. The glass slide rack 8 is provided with a plurality of workstations for placing glass slides. The slide module 21 is slidably installed on the workbench 1. The slide module 21 can move in the X direction and the Y direction at the same time. The X direction is perpendicular to the Y direction. The slide module 21 is located between the workbench 1 and the mounting block 24. The linkage of the slide module 21 in the X and Y directions in the present invention enables the glass slide in the slide module 21 to make any planar motion relative to the drip module 23, that is, the working liquid in the drip module 23 can flow out from the liquid outlet and drip on the glass slide to form a closed circle, blocking the outflow of the impedance reagent in the circle, so that a small amount of impedance reagent can be added to cover the entire tissue surface, so that when the reagent is added to the glass slide later, it plays a role in saving reagents and avoids the use of coverslips. In addition, in the present invention, circles are drawn on the glass slide by dripping liquid, that is, the contact between the drip module 23 and the glass slide is avoided. Considering that the glass slide is repaired when drawing circles, the surface of the glass slide is often wet at this time, and drawing circles with the immunohistochemistry pen in contact with the glass slide will result in unsatisfactory circle drawing effects.

[0055] like Figure 3As shown, the pipetting assembly 3 includes a reagent bottle fixing frame 31, a pipetting needle 32, a pipetting sliding module 33 and a pipetting fixing frame 34. The reagent bottle fixing frame 31 is slidably installed on the workbench 1, and the reagent bottle fixing frame 31 can move along the Y direction on the workbench 1 (the movement between the reagent bottle fixing frame 31 and the workbench 1 can be achieved by means of a screw nut or by being driven by a cylinder, which is not described in detail here), and a plurality of cavities for placing reagent bottles are provided on the reagent bottle fixing frame 31, the pipetting fixing frame 34 is fixedly installed on the workbench 1, the pipetting sliding module 33 is slidably installed on the pipetting fixing frame 34, and the pipetting needle 32 is fixedly installed on the pipetting sliding module 33. The pipetting needle 32 can move along the X direction and the Z direction at the same time, and the Z direction is perpendicular to the X direction and the Y direction respectively. The pipetting needle 32 can absorb the reagent in the reagent bottle and drop the reagent on the carrier sheet. In a preferred embodiment, the pipette needle 32 is connected to the suction pump, and a cavity for accommodating the reagent is provided in the pipette needle 32. In actual use, since the pipette needle 32 can move in the X and Z directions, and the reagent bottle holder 31 can move in the Y direction, the pipette needle 32 can absorb the reagent in the reagent bottle on the reagent bottle holder 31 and transfer the reagent to the glass slide. In a more preferred embodiment, the X direction and the Y direction are two mutually perpendicular directions on the plane where the workbench 1 is located, and the Z direction is perpendicular to the plane where the workbench 1 is located.

[0056] In a preferred embodiment, Figure 3 As shown, the pipetting sliding module 33 includes a pipetting transverse plate 331, a pipetting vertical rod 332 and a pipetting slider 333. The pipetting fixed frame 34 is fixedly installed on the workbench 1. The pipetting transverse plate 331 is slidably set on the pipetting fixed frame 34. The pipetting transverse plate 331 can move along the X direction on the pipetting fixed frame 34. The pipetting vertical rod 332 is fixedly set on the pipetting transverse plate 331 along the Z direction. The pipetting slider 333 is slidably set on the pipetting vertical rod 332. The pipetting needle 32 is fixedly installed on the pipetting slider 333.

[0057] like Figure 2 As shown, the liquid adding tank assembly 4 includes a liquid adding chamber 41 and a liquid adding rack 42 arranged on the workbench 1. The liquid adding chamber 41 is used to place the glass slide rack 8. The liquid adding rack 42 is provided with a plurality of liquid adding needles 43. The outlet of the liquid adding needle 43 is located above the glass slide rack 8, and the inlet of the liquid adding needle 43 is connected to the medicine bottle. In a preferred embodiment, a drainage trough is provided at the bottom of the liquid adding chamber 41. In actual use, when the glass slide needs to be cleaned with medicine, the liquid adding needle 43 can drip the medicine in the medicine bottle onto the glass slide. When the number of slides is small, the liquid adding tank assembly 4 provided by the present invention can clean a single glass slide separately, that is, the glass slide is accurately cleaned by dripping, avoiding the waste of medicine generated when cleaning the glass slide by immersion. When the cleaning is completed, the medicine is discharged from the liquid adding chamber 41 through the drainage trough.

[0058] Furthermore, the novel automated immunohistochemical staining instrument provided by the present invention further comprises a baking sheet component 5, such as Figure 7 and Figure 8 As shown, the baking sheet assembly 5 includes a baking sheet box 50 fixedly mounted on the workbench 1, and the baking sheet box 50 includes a baking sheet cavity 51 and an air guide cavity 52. ​​A first side 511 of the baking sheet cavity 51 is provided with a plurality of strip-shaped notches 512, and a second side and a third side connected to the first side 511 of the baking sheet cavity 51 are respectively provided with baking sheet mounting strips 513 corresponding to the strip-shaped notches 512, and the glass slide rack 8 can enter the baking sheet cavity 51 along the strip-shaped notches 512 and be placed between the two baking sheet mounting strips 513, and a sliding door 514 for controlling the opening and closing of the baking sheet cavity 51 is slidably provided on a side of the baking sheet cavity 51 opposite to the first side 511. The air guide cavity 52 is connected to and communicates with the baking sheet cavity 51, and a heating module and a heat-conducting fan are provided in the air guide cavity 52, and the heat-conducting fan can blow hot air into the baking sheet cavity 51. During actual use, the user inserts the slide rack 8 with the slides into the baking chamber 51 through the strip-shaped gap, so that the slide rack 8 is securely placed between the two baking mounting strips 513. After the slides are sterilized at high temperature for a period of time, the sliding door 514 automatically opens, and the mechanical gripper 73 takes the slide rack 8 out of the baking chamber 51 to proceed to the next step, such as transferring the slide rack 8 to the slide board module 21 for the process of dripping and drawing circles, thereby realizing automated immunohistochemistry operations.

[0059] Furthermore, the novel automated immunohistochemical staining instrument further comprises an incubation component 6, such as Fig. 9 and Fig.10 As shown, the incubation assembly 6 includes a plurality of incubation chambers 60 stacked on the workbench 1, and the incubation chambers 60 are all located below the pipetting fixed frame 34. A heating device 611 is arranged on the outer surface of the bottom plate 61 of the incubation chamber 60, and a plurality of heat dissipation strips 612 are arranged on the inner surface of the bottom plate 61 of the incubation chamber 60. An incubation board for placing a glass slide rack 8 is arranged in the incubation chamber 60. By arranging a plurality of heat dissipation strips 612, the heat is uniform, and the slides on the incubation board can be heated uniformly, so that the slides are in a constant temperature and humidity condition, which is convenient for cell culture and conducive to the experiment. The incubation chamber 60 is provided with a first opening 63, and an ejection motor is arranged on the side plate of the incubation chamber 60 opposite to the first opening 63. The output shaft of the ejection motor is connected to the first screw rod 65, and the first screw rod 65 faces the first opening 63. The axial direction of the first screw rod 65 is the same as the Y direction, and an internal threaded hole matching the first screw rod 65 is arranged on the incubation board. Driven by the ejection motor and the first screw rod 65 , the incubation plate can extend out of the incubation chamber 60 , so that a displacement relative to the Y direction can be generated between the pipette needle 32 and the incubation plate, which is convenient for the dripping of the pipette needle 32 and also facilitates the entry and exit of the slide rack 8 in the incubation chamber 60 .

[0060] In addition, if Figure 1 and Fig.11 As shown, the new automated immunohistochemical staining instrument further includes a transport component 7, which includes a transport frame 71, a transport sliding module 72 and a mechanical gripper 73. The transport frame 71 is fixedly arranged on the workbench 1, the mechanical gripper 73 is fixedly mounted on the transport sliding module 72, the transport sliding module 72 is slidably mounted on the transport frame 71, and the transport sliding module 72 can move in the X direction, the Y direction and the Z direction at the same time.

[0061] The mechanical gripper 73 includes a gripper fixing frame 731 and two clamping rods 732 arranged opposite to each other. The gripper fixing frame 731 is fixedly mounted on the transport sliding module 72. The clamping rods 732 are slidably arranged on the gripper fixing frame 731. The two clamping rods 732 can clamp the glass slide rack 8. The mechanical gripper 73 can clamp the glass slide rack 8 through the clamping rods 732 and transport the glass slide rack 8 to any one of the slide board module 21, the liquid adding chamber 41, the baking chamber 51 and the incubation plate. The mechanical gripper 73 grabs the glass slide rack 8 and moves it on the workbench 1, thereby improving the degree of automation and the throughput of the glass slide. In a preferred embodiment, the two clamping rods 732 can slide relative to each other to achieve the function of clamping the glass slide rack 8, and the sliding of the clamping rods 732 can be driven by a cylinder or a linear motor. In addition, the clamp rod 732 can also be fixedly set on the gripper fixing frame 731, and a deep hole matched with the clamp rod 732 is opened on the glass slide rack 8, and the glass slide rack 8 is driven by extending the clamp rod 732 into the deep hole. In a specific embodiment, the transport sliding module 72 includes a transport transverse plate 721, a transport vertical plate 722 and a transport slider 723. The transport transverse plate 721 is slidably installed on the transport frame 71, and the transport transverse plate 721 can move along the X direction on the transport frame 71, the transport vertical plate 722 is slidably installed on the transport transverse plate 721, and the transport vertical plate 722 can move along the Y direction on the transport transverse plate 721, the transport slider 723 is slidably installed on the transport vertical plate 722, and the transport slider 723 can move along the Z direction on the transport vertical plate 722, and the gripper fixing frame 731 is fixedly installed on the transport slider 723.

[0062] Finally, the slide module 21 drives the slide rack 8 to move in the following manner: Figures 4 to 6 As shown, the slide module 21 includes a first slide 210 and a second slide 211 , and the top surface of the second slide 211 is slidably connected to the bottom surface of the first slide 210 .

[0063] The side of the first slide 210 is provided with an X-guide rail 2101 and an X-axis driving module 2102, and the workbench 1 is provided with an X-sliding block 10 that is slidably connected to the X-guide rail 2101. The direction of the X-guide rail 2101 is consistent with the X-direction, and the X-driving assembly can drive the X-guide rail 2101 to move along the X-direction under the guidance of the X-sliding block 10. In addition, since the bottom surface of the first slide 210 is also provided with a dovetail groove 2106, the width of the notch of the dovetail groove 2106 is smaller than the width of the groove bottom, and the top surface of the second slide 211 is also provided with a slide rail 2112 that matches the dovetail groove 2106, and the directions of the dovetail groove 2106 and the slide rail 2112 are consistent with the Y-direction, the first slide 210 and the second slide 211 can only generate displacement relative to the Y-direction, that is, when the first slide 210 moves along the X-direction, the second slide 211 will also move in the X-direction under the drive of the first slide 210.

[0064] A Y guide rail 2103 and a Y-axis driving module 2104 are arranged on the bottom surface of the first slide plate 210, and the direction of the Y guide rail 2103 is consistent with the Y direction. A Y slider 2111 slidably connected to the Y guide rail 2103 is fixedly arranged on the second slide plate 211, and the Y-axis driving module 2104 can drive the Y slider 2111 to move along the Y direction, that is, the second slide plate 211 can move along the Y direction under the action of the Y-axis driving module 2104, so the second slide plate 211 can move along the X and Y directions at the same time.

[0065] The first slide 210 is provided with a working through hole 2105 that runs through the bottom and top of the first slide 210, and the cross section of the working through hole 2105 is rectangular. The slide holder 8 is placed on the second slide 211 through the working through hole 2105. When the slide holder 8 moves in the X and Y directions simultaneously with the second slide 211, the slide can move in any track in the plane where the X and Y directions are located, which makes it possible to draw closed circles of different shapes according to the different shapes of tissues on the slide when the drip module 23 draws circles, so that the slide plays a role in saving medicine when the primary or secondary antibody agent is subsequently added. In a preferred embodiment, a pit corresponding to the working through hole 2105 can also be set on the surface of the second slide 211 in contact with the first slide 210, and then the slide holder 8 is installed in the pit to play a role in stable installation.

[0066] Among them, the X-axis drive module 2102 includes a first motor 21021, a first fixed plate 21022 and an X-axis screw rod 21023, and the first slide plate 210 is provided with an internal threaded hole matching the X-axis screw rod 21023; the first fixed plate 21022 is fixedly installed on the workbench 1, the first motor 21021 is fixedly installed on one side of the first fixed plate 21022, one end of the X-axis screw rod 21023 is rotatably connected to the output shaft of the first motor 21021, and the other end of the X-axis screw rod 21023 is installed in the internal threaded hole on the first slide plate 210, and the direction of the X-axis screw rod 21023 is consistent with the X direction. The Y-axis driving module 2104 includes a second motor 21041, a second fixed plate 21042 and a Y-axis screw rod 21043. The Y slider 2111 is provided with an internal threaded hole matched with the Y-axis screw rod 21043. One end of the second fixed plate 21042 is fixedly mounted on the bottom surface of the first slide plate 210, and the second motor 21041 is fixedly mounted on one side of the second fixed plate 21042. One end of the Y-axis screw rod 21043 is rotatably connected to the output shaft of the second motor 21041, and the other end of the Y-axis screw rod 21043 is mounted in the internal threaded hole of the Y slider 2111. The direction of the Y-axis screw rod 21043 is consistent with the Y direction. Both the X-axis driving module 2102 and the Y-axis driving module 2104 are driven in the form of screw nuts, which makes the driving process stable and controllable, and the structure is simple and reliable.

[0067] It should be understood that the above description of the specific embodiments of the present invention is only for illustrating the technical route and features of the present invention, and its purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, but the present invention is not limited to the above specific implementation methods. Any changes or modifications made within the scope of the claims of the present invention should be included in the protection scope of the present invention.

Claims

1. An automated immunohistochemical staining instrument, characterized in that: It includes: A workbench, an automatic circle drawing component, a liquid transfer component and a liquid adding tank component, wherein the automatic circle drawing component, the liquid transfer component and the liquid adding tank component are all arranged on the workbench; The automatic circle drawing assembly includes a slide module, a crossbeam and a mounting block for mounting a dripping module, the liquid outlet of the dripping module faces the workbench, the crossbeam is fixedly mounted on the workbench, the mounting block is slidably mounted on the crossbeam, the slide module is used to accommodate a glass slide rack, the glass slide rack is provided with a plurality of stations for placing glass slides, the slide module is slidably mounted on the workbench, the slide module can move in the X direction and the Y direction at the same time, the X direction is perpendicular to the Y direction, and the slide module is located between the workbench and the mounting block; The pipetting assembly includes a reagent bottle fixing frame, a pipetting needle, a pipetting sliding module and a pipetting fixing frame, wherein the reagent bottle fixing frame is slidably mounted on the workbench, the reagent bottle fixing frame can move along the Y direction on the workbench, and a plurality of cavities for placing reagent bottles are provided on the reagent bottle fixing frame, the pipetting fixing frame is fixedly mounted on the workbench, the pipetting sliding module is slidably mounted on the pipetting fixing frame, the pipetting needle is fixedly mounted on the pipetting sliding module, the pipetting needle can move along the X direction and the Z direction at the same time, the Z direction is perpendicular to the X direction and the Y direction respectively, and the pipetting needle can absorb the reagent in the reagent bottle and drop the reagent on the carrier sheet; The liquid adding tank assembly comprises a liquid adding chamber and a liquid adding rack arranged on the workbench, the liquid adding chamber is used to place the slide rack, a plurality of liquid adding needles are arranged on the liquid adding rack, the outlets of the liquid adding needles are located above the slide rack, and the inlets of the liquid adding needles are connected to the medicine bottles; The slide module comprises a first slide and a second slide, wherein the top surface of the second slide is slidably connected to the bottom surface of the first slide; An X-guide rail and an X-axis driving module are arranged on the side of the first slide plate, an X-sliding block slidably connected to the X-guide rail is arranged on the workbench, the direction of the X-guide rail is consistent with the X direction, and the X-axis driving module can drive the X-guide rail to move along the X direction under the guidance of the X-sliding block; A Y guide rail and a Y-axis driving module are arranged on the bottom surface of the first slide plate, the direction of the Y guide rail is consistent with the Y direction, a Y slider slidably connected to the Y guide rail is fixedly arranged on the second slide plate, and the Y-axis driving module can drive the Y slider to move along the Y direction; The first slide plate is provided with a working through hole penetrating the bottom surface and the top surface of the first slide plate, the cross section of the working through hole is rectangular, and the glass slide rack is placed on the second slide plate through the working through hole; The bottom surface of the first slide plate is also provided with a dovetail groove, the width of the dovetail groove is smaller than the width of the groove bottom, and the top surface of the second slide plate is also provided with a slide rail matching the dovetail groove, and the directions of the dovetail groove and the slide rail are consistent with the Y direction.

2. The automated immunohistochemical staining instrument according to claim 1, characterized in that: The automated immunohistochemical staining instrument further comprises a baking component, the baking component comprises a baking box fixedly mounted on the workbench, the baking box comprises a baking cavity and an air guide cavity, a first side of the baking cavity is provided with a plurality of strip-shaped notches, a second side and a third side of the baking cavity connected to the first side are respectively provided with baking installation strips corresponding to the strip-shaped notches, the glass slide rack can enter the baking cavity along the strip-shaped notches and be placed between the two baking installation strips, and a sliding door for controlling the opening and closing of the baking cavity is slidably provided on a side of the baking cavity opposite to the first side; The air guide cavity is connected to and communicates with the slice baking cavity. A heating module and a heat-conducting fan are arranged in the air guide cavity. The heat-conducting fan can blow hot air into the slice baking cavity.

3. The automated immunohistochemical staining instrument according to claim 2, characterized in that: The automated immunohistochemistry staining instrument also includes an incubation component, which includes a plurality of incubation chambers stacked on the workbench, the incubation chambers are all located below the pipetting fixture, a heating device is provided on the outer surface of the bottom plate of the incubation chamber, a plurality of heat dissipation strips are spaced apart on the inner surface of the bottom plate of the incubation chamber, and an incubation plate for placing the slide rack is provided in the incubation chamber.

4. The automated immunohistochemical staining instrument according to claim 3, characterized in that: The incubation chamber is provided with a first opening, and an ejection motor is arranged on the side plate of the incubation chamber on the side opposite to the first opening, the output shaft of the ejection motor is connected to the first screw rod, the first screw rod faces the first opening, and the axial direction of the first screw rod is the same as the Y direction, and the incubation plate is provided with an internal threaded hole matching the first screw rod.

5. The automated immunohistochemical staining instrument according to claim 4, characterized in that: The automated immunohistochemical staining instrument further comprises a transport component, which comprises a transport frame, a transport sliding module and a mechanical gripper; The transfer rack is fixedly arranged on the workbench, the mechanical gripper is fixedly installed on the transfer sliding module, the transfer sliding module is slidably installed on the transfer rack, and the transfer sliding module can move along the X direction, the Y direction and the Z direction at the same time; The mechanical gripper includes a gripper fixing frame and a pair of clamping rods arranged on the gripper fixing frame, the gripper fixing frame is fixedly installed on the transfer sliding module, the clamping rods are slidably arranged on the gripper fixing frame, and the two clamping rods can clamp the glass slide rack, and the mechanical gripper can clamp the glass slide rack through the clamping rods and transport the glass slide rack to any one of the slide board module, the liquid adding chamber, the baking chamber and the incubation plate.

6. The automated immunohistochemical staining instrument according to claim 5, characterized in that: The transfer sliding module includes a transfer horizontal plate, a transfer vertical plate and a transfer sliding block; The transfer transverse plate is slidably installed on the transfer frame, and the transfer transverse plate can move along the X direction on the transfer frame. The transfer vertical plate is slidably installed on the transfer transverse plate, and the transfer vertical plate can move along the Y direction on the transfer transverse plate. The transfer slider is slidably installed on the transfer vertical plate, and the transfer slider can move along the Z direction on the transfer vertical plate. The gripper fixing frame is fixedly installed on the transfer slider.

7. The automated immunohistochemical staining instrument according to any one of claims 1 to 6, characterized in that: The X-axis drive module includes a first motor, a first fixed plate and an X-axis screw rod, the first slide plate is provided with an internal threaded hole matched with the X-axis screw rod; the first fixed plate is fixedly mounted on the workbench, the first motor is fixedly mounted on one side of the first fixed plate, one end of the X-axis screw rod is rotatably connected to the output shaft of the first motor, the other end of the X-axis screw rod is mounted in the internal threaded hole on the first slide plate, and the direction of the X-axis screw rod is consistent with the X direction; The Y-axis driving module includes a second motor, a second fixed plate and a Y-axis screw rod. The Y slider is provided with an internal threaded hole that cooperates with the Y-axis screw rod. One end of the second fixed plate is fixedly mounted on the bottom surface of the first slider, and the second motor is fixedly mounted on one side of the second fixed plate. One end of the Y-axis screw rod is rotatably connected to the output shaft of the second motor, and the other end of the Y-axis screw rod is mounted in the internal threaded hole of the Y slider. The direction of the Y-axis screw rod is consistent with the Y direction.

8. The automated immunohistochemical staining instrument according to any one of claims 1 to 6, characterized in that: The pipetting sliding module includes a pipetting transverse plate, a pipetting vertical rod and a pipetting sliding block. The pipetting fixed frame is fixedly installed on the workbench. The pipetting transverse plate is slidably arranged on the pipetting fixed frame. The pipetting transverse plate can move along the X direction on the pipetting fixed frame. The pipetting vertical rod is fixedly arranged on the pipetting transverse plate along the Z direction. The pipetting sliding block is slidably arranged on the pipetting vertical rod. The pipetting needle is fixedly installed on the pipetting sliding block.

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