Photocrosslinking-based epitope targeting antibody screening method
By introducing photocrosslinkable amino acids into antigen epitopes and using light to form covalent crosslinks, the problem of epitope bias in monoclonal antibody screening was solved, thereby improving antibody sequence diversity and achieving efficient screening of antibodies targeting epitopes.
Patent Information
- Application Number
- CN202180015206.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2020-02-17
- Filing Date
- 2021-02-17
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2041-02-17
AI Technical Summary
Existing technologies suffer from epitope bias in monoclonal antibody screening, making it difficult to efficiently screen antibodies with weak binding strength to target epitopes but good functionality, resulting in insufficient antibody sequence diversity.
By introducing photocrosslinkable amino acids at or near a specific epitope of the target antigen, covalent crosslinks are formed using light irradiation. Antibodies that bind to the target epitope form covalent complexes with the antigen, while unbound antibodies are eluted and separated, thus achieving specific screening of antibodies.
It improves the sequence diversity of candidate antibodies during antibody screening, effectively screening out antibodies with weak binding strength to the target epitope but good functionality, and reducing enrichment bias caused by differences in initial affinity.
Smart Images
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Abstract
Citation Information
Patent Citations
Methods and compositions for the development of antibodies specific to epitope post-translational modification status
US20210032368A1