A fermentation desublimation crystallization device for tetramethylpyrazine

Abstract

The present invention provides a fermentation desublimation crystallization device for tetramethylpyrazine, comprising a fermentation tank, a spiral coil, a rinsing nozzle, and a stirring mechanism. The spiral coil is located at an upper position inside the fermentation tank, the rinsing nozzle is located inside the fermentation tank and above the spiral coil, and the stirring end of the stirring mechanism is located at a lower position inside the fermentation tank. A pH electrode, a temperature electrode, and a dissolved oxygen electrode for monitoring the state of the fermentation liquid are provided on the side wall of the fermentation tank. A discharge pipe is provided at the bottom end of the fermentation tank, and an inoculation port, an air inlet, and an air outlet are provided at the top end of the fermentation tank. The device of the present invention increases the purification efficiency of tetramethylpyrazine, effectively reduces the impurity content of the purified product, and saves the steps and costs of purifying the fermentation liquid.

Description

Technical Field

[0001] The invention relates to the field of bioengineering, and in particular to a fermentation desublimation crystallization device for tetramethylpyrazine. Background Art

[0002] Tetramethylpyrazine is a common heterocyclic nitrogenous compound, widely used in the treatment of food additives and cardiovascular and cerebrovascular diseases, and people are paying more and more attention to it. For better production of tetramethylpyrazine, the production method of tetramethylpyrazine is constantly improving and optimizing, but there are still certain problems. Fermentation purification is the key link in producing tetramethylpyrazine, and this link greatly affects the quality and acquisition of tetramethylpyrazine. At present, tetramethylpyrazine fermentation liquid is usually placed in a fermentor tank, and tetramethylpyrazine is extracted in the fermentation liquid by a biological fermentation method. In the fermentation liquid of the biological fermentation method, tetramethylpyrazine concentration is low, and subsequent purification process can not obtain more tetramethylpyrazine products, and the purification process is complicated, and the purification cost is high. Simultaneously, the solubility of tetramethylpyrazine in the fermentation liquid is very little, and after the fermentation liquid is saturated, crystallization will be produced in the fermentation process and attached to the fermentor tank inner wall, so that the tetramethylpyrazine obtained by simply relying on purification from the fermentation liquid is less, therefore it is necessary to have a device that can improve tetramethylpyrazine purification efficiency and purification amount, purification step is simple and cost-effective. Summary of the Invention

[0003] In order to solve the problems existing in the background technology, the present invention proposes a fermentation desublimation crystallization device of tetramethylpyrazine, including a fermentation tank, a spiral coil, a rinsing nozzle and a stirring mechanism, wherein the spiral coil is located at the upper position inside the fermentation tank, the rinsing nozzle is located inside the fermentation tank, and the rinsing nozzle is located above the spiral coil, the stirring end of the stirring mechanism is located at the lower position inside the fermentation tank, the side wall of the fermentation tank is provided with a pH electrode, a temperature electrode and a dissolved oxygen electrode for monitoring the state of the fermentation liquid, the bottom end of the fermentation tank is provided with a discharge pipe, and the top end of the fermentation tank is provided with an inoculation port, an air inlet and an air outlet.

[0004] Preferably, the spiral coil includes a spiral coil water inlet and a spiral coil water outlet, the spiral coil water inlet and the spiral coil water outlet extend outside the fermentation tank, and the spiral coil water inlet and the spiral coil water outlet are located at the top of the fermentation tank.

[0005] Preferably, an air inlet pipe is provided in the fermentation tank, one end of the air inlet pipe is connected to the air inlet, and the other end of the air inlet pipe extends to a position near the bottom of the fermentation tank, and a plurality of air holes are provided at the end of the air inlet pipe near the bottom of the fermentation tank.

[0006] Preferably, the stirring mechanism includes a motor, a shaft and an anchor frame agitator, the motor is located at the top end of the fermentation tank, the shaft is located inside the fermentation tank, one end of the shaft extends outside the fermentation tank and is connected to the output end of the motor, and the other end of the shaft is connected to the anchor frame agitator, and the anchor frame agitator is located at the lower position inside the fermentation tank.

[0007] Preferably, a jacket is provided below the side wall of the fermentation tank, and the jacket is provided with a jacket water outlet and a jacket water inlet.

[0008] Preferably, a sight glass is provided above the side wall of the fermentation tank.

[0009] Preferably, the top of the fermentation tank is provided with a defoaming agent port, an acid and alkali agent port, a feed port and a rinsing nozzle water inlet, and the rinsing nozzle water inlet end is connected to the rinsing nozzle water inlet.

[0010] Preferably, a discharge valve is provided on the discharge pipe.

[0011] Preferably, a bracket for supporting the fermentation tank is connected below the fermentation tank.

[0012] The present invention has the following beneficial effects: the device of the present invention can control temperature, and the spiral coil increases the surface area inside the fermentation tank. When water at a certain temperature is passed through the spiral coil, tetramethylpyrazine in the fermentation broth can be volatilized and sublimated, and a large number of crystals adhere to the spiral coil, thereby increasing the amount and crystallization rate of tetramethylpyrazine. The crystals are sprayed and dissolved by the elution nozzle, facilitating crystal collection. The device of the present invention increases the purification efficiency of tetramethylpyrazine, effectively reduces the impurity content of the purified product, and saves the steps and costs of fermentation broth purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0014] Figure 2 It is a top view of the present invention.

[0015] Numbers in the figure: 1- bracket, 2- discharge valve, 3- discharge pipe, 4- jacket water inlet, 5- jacket, 6- pH electrode, 7- temperature electrode, 8- dissolved oxygen electrode, 9- sight glass, 10- motor, 11- elution nozzle, 12- fermentation tank, 13- crystallization coil, 14- air inlet pipe, 15- shaft, 16- jacket water outlet, 17- anchor frame agitator, 18- feeding port, 19- defoaming agent port, 20- acid and alkali agent port, 21- crystallization coil water inlet, 22- inoculation port, 23- elution nozzle water inlet, 24- air outlet, 25- air inlet, 26- crystallization coil water outlet. DETAILED DESCRIPTION

[0016] In order to make the technical solutions, purposes and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be understood that the provided embodiments are only one way to implement the technical solutions and do not represent all embodiments. Based on this embodiment, any non-creative embodiments of the present invention made by ordinary technicians in this field are within the scope of protection of the present invention.

[0017] Combined with attachment Figure 1 and 2 A fermentation desublimation crystallization device for tetramethylpyrazine includes a fermentation tank 12, a spiral coil 13, a rinsing nozzle 11 and a stirring mechanism, wherein the spiral coil 13 is located at the upper position inside the fermentation tank 12, the rinsing nozzle 11 is located inside the fermentation tank 12, and the rinsing nozzle 11 is located above the spiral coil 13, and the stirring end of the stirring mechanism is located at the lower position inside the fermentation tank 12. A pH electrode 6, a temperature electrode 7 and a dissolved oxygen electrode 8 for monitoring the state of the fermentation liquid are provided on the side wall of the fermentation tank 12, wherein the pH electrode 6 is used to monitor the pH value of the fermentation liquid, the temperature electrode 7 is used to monitor the temperature of the fermentation liquid, and the dissolved oxygen electrode 8 is used to monitor the dissolved oxygen content of the fermentation liquid. A discharge pipe 3 is provided at the bottom end of the fermentation tank 12, and an inoculation port 22, an air inlet 25 and an air outlet 24 are provided at the top end of the fermentation tank 12.

[0018] Specifically, the spiral coil 13 includes a spiral coil water inlet 21 and a spiral coil water outlet 26 , which extend outside the fermentation tank 12 and are located at the top of the fermentation tank 12 .

[0019] Specifically, an air intake pipe 14 is provided in the fermentation tank 12, one end of the air intake pipe 14 is connected to the air inlet 25, and the air intake pipe 14 is connected to an air compressor through the air inlet 25. The air compressor is responsible for introducing air into the fermentation tank 12, and the other end of the air intake pipe 14 extends to a position near the bottom of the fermentation tank 12. A plurality of air holes are provided at one end of the air intake pipe 14 near the bottom of the fermentation tank 12.

[0020] Specifically, the stirring mechanism includes a motor 10, a shaft 15 and an anchor frame agitator 17. The motor 10 is located at the top end of the outside of the fermentation tank 12, and the shaft 15 is located inside the fermentation tank 12. One end of the shaft 15 extends to the outside of the fermentation tank 12 and is connected to the output end of the motor 10. The other end of the shaft 15 is connected to the anchor frame agitator 17. The anchor frame agitator 17 is located at the lower position inside the fermentation tank 12.

[0021] Specifically, a jacket 5 is provided below the side wall of the fermentation tank 12 , and a jacket water outlet 16 and a jacket water inlet 4 are provided on the jacket 5 . The jacket 5 is responsible for heating and cooling the fermentation tank 12 to maintain the temperature environment for fermentation.

[0022] Specifically, a sight glass 9 is provided above the side wall of the fermentation tank 12 , through which the crystallization and dissolution of the crystals in the fermentation tank 12 can be observed.

[0023] Specifically, the top of the fermentation tank 12 is provided with a defoaming agent port 19, an acid and alkali agent port 20, a feeding port 18 and a rinsing nozzle water inlet 23. The water inlet end of the rinsing nozzle 11 is connected to the rinsing nozzle water inlet 23. The defoaming agent is added during the fermentation process through the defoaming agent port 19, the acid and alkali agent port 20 is used to add acid or alkali during the fermentation process, and the substrate is added during the fermentation process through the feeding port 18.

[0024] Specifically, a discharge valve 2 is provided on the discharge pipe 3 .

[0025] Specifically, a bracket 1 for supporting the fermentation tank 12 is connected below the fermentation tank 12 .

[0026] Working process: The fermentation seed liquid is inoculated into the fermentation tank 12 containing sterile fermentation liquid through the inoculation port 22, the pH electrode 6, the temperature electrode 7 and the dissolved oxygen electrode 8 are turned on, and the motor 10 is turned on at the same time. The motor 10 drives the anchor frame stirrer 17 to rotate through the shaft 15 to stir the fermentation liquid to promote fermentation. During the fermentation process, the tetramethylpyrazine in the fermentation liquid can easily reach saturation. After saturation, the tetramethylpyrazine will evaporate to the upper part of the fermentation tank 12. At this time, water at 15-20°C is introduced into the crystallization coil 13 through the crystallization coil water inlet 21, so that the temperature of the upper part of the fermentation tank 12 can allow the volatilized tetramethylpyrazine to sublime, and a large amount of crystals are attached to the spiral coil 13. After the fermentation is completed, the discharge valve 2 is opened and the fermentation liquid is discharged through the discharge pipe 3. After the fermentation liquid is discharged, the discharge valve 2 is closed and hot water is injected into the water inlet 23 of the rinsing nozzle. The hot water is sprayed out through the rinsing nozzle 11 at the top of the fermentation tank 12 to spray and dissolve the crystals. The dissolved crystals flow to the bottom of the fermentation tank 12. The discharge valve 2 is opened again to collect the dissolved crystals to facilitate the next step of purification and recovery of tetramethylpyrazine.

[0027] The above embodiments merely illustrate the basic principles and features of the present invention and are not intended to be limiting. It should be understood that various changes and modifications may be made to the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and such changes and modifications are intended to fall within the scope of the present invention as claimed. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fermentation desublimation crystallization device for tetramethylpyrazine, characterized in that: The invention comprises a fermentation tank (12), a spiral coil (13), a rinsing nozzle (11) and a stirring mechanism, wherein the spiral coil (13) is located at an upper position inside the fermentation tank (12), the rinsing nozzle (11) is located inside the fermentation tank (12), and the rinsing nozzle (11) is located above the spiral coil (13), and the stirring end of the stirring mechanism is located at a lower position inside the fermentation tank (12), a pH electrode (6), a temperature electrode (7) and a dissolved oxygen electrode (8) for monitoring the state of the fermentation liquid are provided on the side wall of the fermentation tank (12), a discharge pipe (3) is provided at the bottom end of the fermentation tank (12), and an inoculation port (22), an air inlet (25) and an air outlet (24) are provided at the top end of the fermentation tank (12); The spiral coil (13) includes a spiral coil water inlet (21) and a spiral coil water outlet (26), the spiral coil water inlet (21) and the spiral coil water outlet (26) extending outside the fermentation tank (12), and the spiral coil water inlet (21) and the spiral coil water outlet (26) are located at the top of the fermentation tank (12); An air inlet pipe (14) is provided in the fermentation tank (12), one end of the air inlet pipe (14) is connected to the air inlet (25), and the other end of the air inlet pipe (14) extends to a position near the bottom of the fermentation tank (12), and a plurality of air holes are provided at one end of the air inlet pipe (14) near the bottom of the fermentation tank (12); The stirring mechanism comprises a motor (10), a shaft (15) and an anchor frame stirrer (17), wherein the motor (10) is located at the top end of the outside of the fermentation tank (12), the shaft (15) is located inside the fermentation tank (12), one end of the shaft (15) extends outside the fermentation tank (12) and is connected to the output end of the motor (10), and the other end of the shaft (15) is connected to the anchor frame stirrer (17), and the anchor frame stirrer (17) is located at a lower position inside the fermentation tank (12); The top of the fermentation tank (12) is provided with a defoaming agent port (19), an acid and alkali agent port (20), a feed port (18) and a rinsing nozzle water inlet (23), and the water inlet end of the rinsing nozzle (11) is connected to the rinsing nozzle water inlet (23).

2. The fermentation desublimation crystallization device of tetramethylpyrazine according to claim 1, characterized in that: A jacket (5) is provided below the side wall of the fermentation tank (12), and the jacket (5) is provided with a jacket water outlet (16) and a jacket water inlet (4).

3. The fermentation desublimation crystallization device of tetramethylpyrazine according to claim 1, characterized in that: A sight glass (9) is provided above the side wall of the fermentation tank (12).

4. The fermentation desublimation crystallization device of tetramethylpyrazine according to claim 1, characterized in that: A discharge valve (2) is provided on the discharge pipe (3).

5. The fermentation desublimation crystallization method of tetramethylpyrazine according to claim 1, characterized in that: A bracket (1) for supporting the fermentation tank (12) is connected below the fermentation tank (12).

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