Protein compositions having isoprene polymerization activity and uses thereof

By synthesizing a specific protein composition to express isoprene polymerization in a lipid membrane, the problems of resource instability and uneven quality in natural rubber production have been solved, achieving efficient and stable natural rubber production.

CN115698293BActive Publication Date: 2026-04-28SUMITOMO RIKO CO LTD
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Patent Information

Application Number
CN202180035798.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2020-10-30
Filing Date
2021-10-21
Publication Date
2026-04-28
Estimated Expiration
2041-10-21

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the efficient production of highly stable natural rubber, and the collection and processing of rubber particles are complex, leading to unstable resource supply and uneven quality.

Method used

Natural rubber is produced by synthesizing protein compositions containing specific amino acid sequences and activity similarities, expressing these proteins in lipid membranes using a cell-free protein production system to form lipid membrane constructs, and then carrying out isoprene polymerization.

Benefits of technology

It has enabled efficient and stable production of natural rubber, solved the problems of unstable resource supply and uneven quality, and met global demand.

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Abstract

The present invention provides a means for efficiently producing natural rubber and achieving stable securing, with the aim of securing rubber resources. The present invention provides a protein composition comprising (A-1) a protein having the same activity as CPT6 or (A-2) a protein having the same activity as CPT7 and comprising (B) a protein having the same activity as CPTL, a lipid membrane construct comprising the above composition, a method for producing the same, a cell expressing the proteins constituting the above composition, a method for producing the same, and a method for producing an isoprene polymer compound using any one of these.
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