A sterilization device and method for antibody preparation

Abstract

The present invention provides a sterilization device and method for antibody preparation, which belongs to the technical field of antibody preparation. The sterilization device for antibody preparation includes a body, a filter assembly, and a temporary storage assembly. The body includes a main body and a partition that divides the main body into an upper accommodating chamber and a lower accommodating chamber. The main body is located on the upper accommodating chamber and has a delivery hole. The partition is located on one side of the lower accommodating chamber and is equipped with a relative radiation sterilizer. The partition has a connecting hole that connects the upper accommodating chamber and the lower accommodating chamber. The filter assembly is arranged in the upper accommodating chamber. The filter assembly includes a connecting pipe and an ultrafiltration membrane and a temporary storage bucket arranged in sequence from top to bottom in the connecting pipe. The present invention can further reduce the bacterial content and ensure the purity of the antibody as much as possible. It also avoids the harm caused by high-temperature sterilization to the antibody.

Description

Technical Field

[0001] The present invention belongs to the technical field of antibody preparation, and in particular relates to a sterilization device and method for antibody preparation. Background Art

[0002] In recent years, with the advancement of protein technology and recombinant DNA technology, researchers have been able to obtain artificial recombinant antibodies that bind to specific locations, possess various specificities and affinities, and tolerate specific temperatures, pH levels, and organic solvents through research into the genetic nature of antibody production, screening techniques for recombinant antibodies, and direct, site-directed gene manipulation. Typically, an animal is immunized with an antigen. After a certain period of time, the spleen is removed under sterile conditions and total RNA from the splenocytes is extracted. Using cDNA synthesized from reverse transcription of the RNA as a template, the antibody is amplified by PCR, and the light and heavy chains of the antibody are linked to form a single-chain variable fragment (ScFv). The PCR-amplified ScFv fragment is enzymatically digested and ligated to a phage vector. The fragment is then transformed into Escherichia coli or other organisms using conventional methods. The recombinant antibody is then obtained by artificially culturing E. coli harboring the phage-transfected antibody.

[0003] However, in order to effectively utilize biomaterials, researchers often need to sterilize them. While high-temperature sterilization is often used in biomaterial processing, high temperatures can damage biomaterials. Therefore, researchers typically use methods such as ultraviolet light to sterilize biomaterials. However, this method is not thorough enough to sterilize biomaterials, making them more susceptible to contamination and potentially leading to failure in antibody preparation. Summary of the Invention

[0004] The embodiments of the present invention provide a sterilization device and method for antibody preparation, which aims to solve the problem that existing methods such as ultraviolet lamps are not thorough enough in sterilizing biological materials and are more likely to cause contamination to the biological materials.

[0005] In view of the above problems, the technical solution proposed by the present invention is:

[0006] In a first aspect, the present invention provides a sterilization device for antibody preparation, comprising:

[0007] The machine body includes a main body and a partition that is divided into an upper accommodating chamber and a lower accommodating chamber. The main body is located in the upper accommodating chamber and has a delivery hole. The partition is located on one side of the lower accommodating chamber and is equipped with an opposing radiation sterilizer. The partition has a communication hole that connects the upper accommodating chamber and the lower accommodating chamber.

[0008] A filter assembly is disposed in the upper accommodating chamber, and includes a connecting pipe, an ultrafiltration membrane, and a temporary storage bucket disposed in the connecting pipe in order from top to bottom;

[0009] A temporary storage assembly is provided in the lower accommodating chamber, and includes an inner shell, an arc-shaped plate integrally formed with the inner shell, a support hole provided on the arc-shaped plate, and a flexible basin placed in the support hole;

[0010] Wherein, the two radiation sterilizers are both arranged obliquely along the flexible basin.

[0011] As a preferred technical solution of the present invention, the connecting tube has a resting end, and the ultrafiltration membrane has a supporting end; wherein the ultrafiltration membrane is rested on the resting end of the connecting tube through the supporting end.

[0012] As a preferred technical solution of the present invention, the resting end has a protrusion, and the supporting end has a groove adapted to the protrusion; wherein the supporting end is constructed to limit the position of the ultrafiltration membrane through the groove and the protrusion of the resting end.

[0013] As a preferred technical solution of the present invention, the temporary storage bucket is located in the communicating hole and has a discharge end, and a solenoid valve is installed on the discharge end.

[0014] As a preferred technical solution of the present invention, it also includes a swinging mechanism, which includes a orifice plate, a first transmission assembly provided on the orifice plate, and a first drive motor connected to the first transmission assembly; the filter assembly is located within the through hole of the orifice plate, the first transmission assembly is connected to the connecting pipe, and the first drive motor is installed on the orifice plate.

[0015] As a preferred technical solution of the present invention, the first transmission assembly includes a semicircular gear and a first gear and a second gear located between the semicircular gear and the orifice plate, the output end of the first drive motor is fixed to the first gear, and the first gear and the second gear are both provided with a plurality of drive rods in a circular array; wherein the plurality of drive rods on the first gear and the plurality of drive rods on the second gear are configured to be staggered.

[0016] As a preferred technical solution of the present invention, it also includes a jacking mechanism, the jacking mechanism includes a support frame and a reciprocating assembly arranged on the support frame, the support frame has a first connecting hole and a second connecting hole, the reciprocating assembly includes a reciprocating rod and a connecting column sleeved on the reciprocating rod, the reciprocating rod has a first end and a second end, the first end passes through the first connecting hole, and the second end passes through the second connecting hole, the support frame is provided with a positioning rod, the connecting column is provided with an annular groove, and the positioning rod is slidably connected to the annular groove.

[0017] As a preferred technical solution of the present invention, the jacking mechanism also includes a second transmission assembly, the second transmission assembly includes a first rotating wheel, a second rotating wheel and a transmission toothed belt that connects the first rotating wheel and the second rotating wheel, the first rotating wheel and the first end, the first end and the first connecting hole, and the second end and the second connecting hole are all connected through a connecting assembly; wherein, the connecting assembly includes a connecting ring and a bearing sleeved on the surface of the connecting ring, the connecting ring has a linear slide groove on the surface opposite to the reciprocating rod, and the reciprocating rod has a slider adapted to the linear slide groove.

[0018] As a preferred technical solution of the present invention, the lifting mechanism further includes a second drive motor and a side support seat for fixing the support frame to the inner shell, and the output end of the second drive motor is fixed to the second rotating wheel.

[0019] In a second aspect, the present invention provides a sterilization method for antibody preparation, comprising the following steps:

[0020] S1, transporting the biological material from the delivery hole to the ultrafiltration membrane;

[0021] S2, after filtration by ultrafiltration membrane, bacteria and impurities are adsorbed on the ultrafiltration membrane, and the biological materials fall into the temporary storage hopper for temporary storage;

[0022] In S3, the biological material is transported from the discharge end to the flexible basin, and the residual bacteria in the biological material are sterilized again by a radiation sterilizer.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The characteristics of the ultrafiltration membrane are used to filter out bacteria and impurities contained in the biomaterial to reduce the bacteria contained in the biomaterial; then, the biomaterial will be transported to a flexible basin and sterilized with a radiation sterilizer to disinfect the remaining bacteria in the biomaterial. This can further reduce the bacterial content and ensure the purity of the antibody as much as possible. It also avoids the damage of high-temperature sterilization to the antibody production.

[0025] (2) The first drive motor drives the first transmission assembly to transmit to the connecting pipe, so that the connecting pipe can rotate back and forth, thereby enabling the ultrafiltration membrane to achieve swing filtration, making the filtration of bacteria and impurities in the biological material more thorough.

[0026] (3) When the reciprocating rod reciprocates, the arc portion of the reciprocating rod can push the bottom of the flexible basin to be concave, so that the biological material in the flexible basin flows, thereby enabling the radiation sterilizer to better sterilize the biological material.

[0027] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are specifically listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a three-dimensional diagram of a sterilization device for antibody preparation disclosed in the present invention;

[0029] Figure 2 This is a cross-sectional view of a sterilization device for antibody preparation disclosed in the present invention;

[0030] Figure 3 This is a schematic structural diagram of a filter assembly of a sterilization device for antibody preparation disclosed in the present invention;

[0031] Figure 4 yes Figure 3 Cross-sectional view at AA in the middle;

[0032] Figure 5 This is a schematic structural diagram of a temporary storage component of a sterilization device for antibody preparation disclosed in the present invention;

[0033] Figure 6 This is an exploded view of a temporary storage component of a sterilization device for antibody preparation disclosed in the present invention;

[0034] Figure 7 This is a schematic structural diagram of a reciprocating assembly of a sterilization device for antibody preparation disclosed in the present invention;

[0035] Figure 8 This is a schematic structural diagram of a connecting ring of a sterilization device for antibody preparation disclosed in the present invention;

[0036] Figure 9 This is a vertical cross-sectional view of a lifting mechanism of a sterilization device for antibody preparation disclosed in the present invention;

[0037] Figure 10 The present invention discloses a flow chart of a sterilization method for antibody preparation.

[0038] Explanation of reference numerals: 10, body; 101, upper accommodating chamber; 1011, delivery hole; 102, lower accommodating chamber; 1021, revolving door; 10211, pull plate; 110, body; 111, cylinder; 112, top cover; 120, partition; 121, communicating hole; 130, radiation sterilizer; 20, filter assembly; 21, connecting pipe; 211, shelf end; 2111, protrusion; 22, ultrafiltration membrane; 221, supporting end; 2211, groove; 23, temporary storage bucket; 231, discharge end; 30, temporary storage assembly; 31, inner shell; 311, flange; 32, curved plate; 33, supporting hole; 34, flexible basin; 40, swing mechanism; 41, orifice plate; 42, first transmission assembly; 421, Semicircular gear; 422, first gear; 4221, drive rod; 423, second gear; 43, first drive motor; 50, lifting mechanism; 51, support frame; 511, first connecting hole; 512, second connecting hole; 513, positioning rod; 52, reciprocating assembly; 521, reciprocating rod; 5211, first end; 52111, arc portion; 5212, second end; 5213, slider; 522, connecting column; 5221, annular slide; 53, connecting assembly; 531, connecting ring; 5311, linear slide; 532, bearing; 54, second transmission assembly; 541, first rotating wheel; 542, second rotating wheel; 543, transmission toothed belt; 55, second drive motor; 56, side support seat. DETAILED DESCRIPTION

[0039] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0040] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are also within the scope of protection of the present invention.

[0041] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not require further definition or explanation in subsequent drawings.

[0042] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0043] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature identified as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

[0044] Example

[0045] Refer to the attached Figures 1 to 9 As shown, the present invention provides a technical solution: a sterilization device for antibody preparation, including a body 10, a filter assembly 20 and a temporary storage assembly 30; the body 10 includes a main body 110 and a partition 120 that divides the main body 110 into an upper accommodating chamber 101 and a lower accommodating chamber 102, the main body 110 is located on the upper accommodating chamber 101 and has a delivery hole 1011, the partition 120 is located on one side of the lower accommodating chamber 102 and is installed with an opposing radiation sterilizer 130, the partition 120 has a connecting hole 121 that connects the upper accommodating chamber 101 and the lower accommodating chamber 102; the filter assembly 20 is arranged in the upper accommodating chamber 101, The filter assembly 20 includes a connecting pipe 21 and an ultrafiltration membrane 22 and a temporary storage bucket 23 arranged in sequence from top to bottom in the connecting pipe 21; the temporary storage assembly 30 is arranged in the lower accommodating chamber 102, and the temporary storage assembly 30 is arranged in the lower accommodating chamber 102. The temporary storage assembly 30 includes an inner shell 31, an arc-shaped plate 32 integrally formed with the inner shell 31, a support hole 33 provided on the arc-shaped plate 32 and a flexible basin 34 placed in the support hole 33; for example, both radiation sterilizers 130 are arranged obliquely along the flexible basin 34; the radiation sterilizer 130 can be a γ-ray sterilizer 130; the flexible basin 34 is made of flexible rubber material.

[0046] Specifically, the ultrafiltration membrane 22 utilizes its properties to filter out bacteria and impurities contained in the biomaterial, thereby reducing the bacterial content. The biomaterial is then transferred to the flexible basin 34, where the radiation sterilizer 130 sterilizes any remaining bacteria. This further reduces the bacterial content and maximizes the purity of the antibodies.

[0047] In addition, after the flexible basin 34 is placed in the support hole 33, the flexible basin 34 can be fastened to the support hole 33 by means of a clip or a latch, thereby enabling the flexible basin 34 to be assembled and disassembled.

[0048] In some embodiments, the body 110 includes a barrel 111 and a top cover 112 . The top cover 112 is detachably connected to the barrel 111 , for example, by a threaded connection, a latch connection, or a snap-fit connection. This allows the top cover 112 to be easily disassembled and assembled, enabling replacement of the ultrafiltration membrane 22 .

[0049] In some embodiments, the barrel 111 is located in the lower accommodating chamber 102 and has a revolving door 1021, which is provided with a pull plate 10211. For example, the revolving door 1021 is hinged to the barrel 111. By opening and closing the revolving door 1021, not only can the flexible basin 34 be easily replaced, but the reciprocating mechanism can also be easily inspected and repaired.

[0050] In some embodiments, the connecting tube 21 has a resting end 211, and the ultrafiltration membrane 22 has a supporting end 221; for example, the ultrafiltration membrane 22 is placed on the resting end 211 of the connecting tube 21 via the supporting end 221. The ultrafiltration membrane 22 is supported on the resting end 211 via the supporting end 221, so that the ultrafiltration membrane 22 is configured to be freely detachable from the connecting tube 21, thereby facilitating cleaning of the ultrafiltration membrane 22.

[0051] In some embodiments, the resting end 211 has a protrusion 2111, and the supporting end 221 has a groove 2211 that matches the protrusion 2111. For example, the supporting end 221 is configured to limit the position of the ultrafiltration membrane 22 through the groove 2211 and the protrusion 2111 of the resting end 211. When the connecting tube 21 is rotated, the supporting end 221 of the ultrafiltration membrane 22 can remain stationary due to the cooperation between the groove 2211 and the protrusion 2111 of the resting end 211, and the ultrafiltration membrane 22 can swing with the reciprocating motion, thereby allowing the ultrafiltration membrane 22 to filter the biological material more thoroughly.

[0052] In some embodiments, the temporary storage hopper 23 is located within the communication hole 121 and has a discharge port 231. A solenoid valve is installed on the discharge port 231. The solenoid valve allows the discharge port 231 to be opened and closed. Specifically, when the biomaterial is being filtered, the solenoid valve is closed, allowing the biomaterial to be temporarily stored in the temporary storage hopper 23. When the biomaterial is filtered, the solenoid valve is opened, allowing the biomaterial to be transferred to the flexible basin 34.

[0053] In some embodiments, a swing mechanism 40 is further included, comprising an orifice plate 41, a first transmission assembly 42 disposed on the orifice plate 41, and a first drive motor 43 connected to the first transmission assembly 42. The filter assembly 20 is located within the through-hole of the orifice plate 41, the first transmission assembly 42 is connected to the connecting pipe 21, and the first drive motor 43 is mounted on the orifice plate 41. For example, the connecting pipe 21 and the through-hole of the orifice plate 41 may be connected via a bearing 532.

[0054] Refer to the attached Figures 2-4 As shown, in this embodiment, the first drive motor 43 drives the first transmission assembly 42 to transmit to the connecting pipe 21, so that the connecting pipe 21 can rotate back and forth, thereby enabling the ultrafiltration membrane 22 to achieve swing filtration.

[0055] In some embodiments, the first transmission assembly 42 includes a semicircular gear 421, and a first gear 422 and a second gear 423 located between the semicircular gear 421 and the orifice plate 41. The output end of the first drive motor 43 is fixed to the first gear 422. The first gear 422 and the second gear 423 are each provided with a plurality of drive rods 4221 arranged in a circular array. The plurality of drive rods 4221 on the first gear 422 and the plurality of drive rods 4221 on the second gear 423 are arranged in a staggered arrangement. When the first drive motor 43 is driven, if the first gear 422 rotates counterclockwise, the drive rods 4221 of the first gear 422 can push the semicircular gear 421 to rotate clockwise until the drive rods 4221 of the first gear 422 can no longer push the semicircular gear 421. During this process, the drive rods 4221 of the second gear 423 can push the semicircular gear 421 to rotate counterclockwise, thereby achieving reciprocating motion of the connecting tube 21.

[0056] For example, the first transmission assembly 42 can also be a combination of gears. Specifically, a larger-diameter gear is sleeved onto the connecting tube 21, while a smaller-diameter gear meshes with the larger-diameter gear. The output end of the first drive motor 43 is fixed to the axis of the smaller-diameter gear. In this case, the connecting tube 21 can also be rotated back and forth by rotating the first drive motor 43 forward and reverse.

[0057] In some embodiments, a lifting mechanism 50 is also included, which includes a support frame 51 and a reciprocating assembly 52 arranged on the support frame 51. The support frame 51 has a first connecting hole 511 and a second connecting hole 512. The reciprocating assembly 52 includes a reciprocating rod 521 and a connecting column 522 mounted on the reciprocating rod 521. The reciprocating rod 521 has a first end 5211 and a second end 5212. The first end 5211 passes through the first connecting hole 511, and the second end 5212 passes through the second connecting hole 512. A positioning rod 513 is provided on the support frame 51, and an annular groove 5221 is provided on the connecting column 522. The positioning rod 513 is slidably connected to the annular groove 5221.

[0058] Refer to the attached Figures 5 to 9 As shown, in this embodiment, when the second transmission assembly 54 transmits power to the reciprocating rod 521, the reciprocating rod 521 and the connecting column 522 rotate together. The connecting column 522 cooperates with the positioning rod 513 through the annular groove 5221, and the connecting column 522 enables the reciprocating rod 521 to achieve linear reciprocating motion.

[0059] In one embodiment, the end of the first end 5211 near the flexible basin 34 is a circular arc portion 52111. During the reciprocating motion of the reciprocating rod 521, the circular arc portion 52111 of the reciprocating rod 521 can push the bottom of the flexible basin 34 to be concave, thereby causing the biomaterial in the flexible basin 34 to flow, thereby enabling the radiation sterilizer 130 to better sterilize the biomaterial. Furthermore, the circular arc portion 52111 can reduce damage to the flexible basin 34.

[0060] In some embodiments, the lifting mechanism 50 also includes a second transmission assembly 54, the second transmission assembly 54 includes a first rotating wheel 541, a second rotating wheel 542 and a transmission toothed belt 543 that connects the first rotating wheel 541 and the second rotating wheel 542, the first rotating wheel 541 and the first end 5211, the first end 5211 and the first connecting hole 511, and the second end 5212 and the second connecting hole 512 are all connected through the connecting assembly 53; wherein, the connecting assembly 53 includes a connecting ring 531 and a bearing 532 mounted on the surface of the connecting ring 531, the connecting ring 531 has a linear slide groove 5311 on the surface opposite to the reciprocating rod 521, and the reciprocating rod 521 has a slider 5213 adapted to the linear slide groove 5311.

[0061] Refer to the attached Figures 5 to 9 As shown, in this embodiment, when the second rotating wheel 542 is driven, the transmission toothed belt 543 drives the first rotating wheel 541 to rotate, thereby transmitting power to the reciprocating rod 521. Simultaneously, during the linear reciprocating motion of the reciprocating rod 521, the cooperation between the bearing 532 and the connecting ring 531, as well as the cooperation between the slider 5213 and the linear guide groove 5311, enables both rotation of the reciprocating rod 521 and linear motion.

[0062] For example, the second transmission assembly 54 may also be a combination of gear sets, with the larger diameter gear connected to the first end 5211 via the connecting assembly 53, and the axis of the smaller diameter gear fixed to the second drive motor 55. The gear set can have a smaller transmission ratio, so that the reciprocating rod 521 can reciprocate slowly, thereby reducing the impact on the flexible basin 34.

[0063] In some embodiments, the lifting mechanism 50 further includes a second drive motor 55 and a side support 56 for fixing the support frame 51 to the inner housing 31. The output end of the second drive motor 55 is fixed to the second rotating wheel 542. The second drive motor 55 can transmit kinetic energy to the second transmission assembly 54.

[0064] It should be noted that the annular groove 5221 and the slider 5213 are determined according to the stroke of the reciprocating rod 521. At the same time, the depression at the bottom of the flexible basin 34 cannot exceed half the height of the flexible basin 34. Therefore, the stroke of the reciprocating rod 521 is determined according to the above.

[0065] Refer to the attached Figure 10 As shown, another embodiment of the present invention provides a sterilization method for antibody preparation, comprising the following steps:

[0066] S1, delivering the biological material from the delivery hole 1011 to the ultrafiltration membrane 22;

[0067] S2, after filtration by the ultrafiltration membrane 22, bacteria and impurities are adsorbed on the ultrafiltration membrane 22, and the biological material falls into the temporary storage hopper 23 for temporary storage;

[0068] S3 , the biological material is transported from the discharge end 231 to the flexible basin 34 , and the residual bacteria in the biological material are sterilized again by the radiation sterilizer 130 .

[0069] It should be noted that the models and specifications of the radiation sterilizer 130, the first drive motor 43 and the second drive motor 55 need to be selected and determined according to the actual specifications of the device. The specific selection calculation method adopts the existing technology in this field, so it will not be described in detail.

[0070] The power supply and principles of the radiation sterilizer 130 , the first drive motor 43 and the second drive motor 55 are clear to those skilled in the art and will not be described in detail here.

[0071] The above is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A sterilization device for antibody preparation, characterized in that: include: The machine body includes a main body and a partition that is divided into an upper accommodating chamber and a lower accommodating chamber. The main body is located in the upper accommodating chamber and has a delivery hole. The partition is located on one side of the lower accommodating chamber and is equipped with an opposing radiation sterilizer. The partition has a communication hole that connects the upper accommodating chamber and the lower accommodating chamber. A filter assembly is disposed in the upper accommodating chamber, and includes a connecting pipe, an ultrafiltration membrane, and a temporary storage bucket disposed in the connecting pipe in order from top to bottom; A temporary storage assembly is provided in the lower accommodating chamber, and includes an inner shell, an arc-shaped plate integrally formed with the inner shell, a support hole provided on the arc-shaped plate, and a flexible basin placed in the support hole; Wherein, the two radiation sterilizers are both arranged obliquely along the flexible basin; The jacking mechanism is further included, the jacking mechanism including a support frame and a reciprocating assembly arranged on the support frame, the support frame having a first connecting hole and a second connecting hole, the reciprocating assembly including a reciprocating rod and a connecting column sleeved on the reciprocating rod, the reciprocating rod having a first end and a second end, the first end passing through the first connecting hole, the second end passing through the second connecting hole, the support frame being provided with a positioning rod, the connecting column being provided with an annular slide groove, the positioning rod being slidably connected to the annular slide groove; The end of the first end close to the flexible basin is an arc portion. When the reciprocating rod reciprocates, the arc portion of the reciprocating rod can push the bottom of the flexible basin to be concave, so as to cause the biomaterial in the flexible basin to flow.

2. The sterilization device for antibody preparation according to claim 1, characterized in that: The connecting tube has a resting end therein, and the ultrafiltration membrane has a supporting end thereon; wherein the ultrafiltration membrane is rested on the resting end of the connecting tube through the supporting end.

3. The sterilization device for antibody preparation according to claim 2, characterized in that: The resting end has a protrusion, and the supporting end has a groove matched with the protrusion; wherein the supporting end is constructed to limit the position of the ultrafiltration membrane through the groove and the protrusion of the resting end.

4. The sterilization device for antibody preparation according to claim 1, characterized in that: The temporary storage bucket is located in the communicating hole and has a discharge end, and a solenoid valve is installed on the discharge end.

5. The sterilization device for antibody preparation according to claim 1, characterized in that: It also includes a swinging mechanism, which includes a orifice plate, a first transmission assembly provided on the orifice plate, and a first drive motor connected to the first transmission assembly; the filter assembly is located within the through hole of the orifice plate, the first transmission assembly is connected to the connecting pipe, and the first drive motor is installed on the orifice plate.

6. The sterilization device for antibody preparation according to claim 5, characterized in that: The first transmission assembly includes a semicircular gear and a first gear and a second gear located between the semicircular gear and the orifice plate. The output end of the first drive motor is fixed to the first gear. The first gear and the second gear are both provided with a plurality of drive rods in a circular array; wherein the plurality of drive rods on the first gear and the plurality of drive rods on the second gear are configured to be staggered.

7. The sterilization device for antibody preparation according to claim 6, characterized in that: The lifting mechanism also includes a second transmission assembly, which includes a first rotating wheel, a second rotating wheel and a transmission toothed belt that connects the first rotating wheel and the second rotating wheel, and the first rotating wheel and the first end, the first end and the first connecting hole, and the second end and the second connecting hole are all connected through a connecting assembly; wherein, the connecting assembly includes a connecting ring and a bearing sleeved on the surface of the connecting ring, a linear slide groove is provided on the surface of the connecting ring opposite to the reciprocating rod, and the reciprocating rod has a slider adapted to the linear slide groove.

8. The sterilization device for antibody preparation according to claim 7, characterized in that: The lifting mechanism further includes a second driving motor and a side support seat for fixing the support frame to the inner shell, and the output end of the second driving motor is fixed to the second rotating wheel.

9. A sterilization method for antibody preparation, applied to the sterilization device for antibody preparation according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1, transporting the biological material from the delivery hole to the ultrafiltration membrane; S2, after filtration by ultrafiltration membrane, bacteria and impurities are adsorbed on the ultrafiltration membrane, and the biological materials fall into the temporary storage hopper for temporary storage; In S3, the biological material is transported from the discharge end to the flexible basin, and the residual bacteria in the biological material are sterilized again by a radiation sterilizer.

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