A freezing device and freezing method for high-throughput freezing of porcine semen

Abstract

The present invention discloses a freezing device and a freezing method for high-throughput frozen pig semen, belonging to the technical field of freezing, and solves the problem that the existing frozen semen transported by large freezing trucks has relatively high consumption and maintenance costs. The device body includes a freezing box and a liquid nitrogen storage box; a first liquid level sensor is arranged in the liquid nitrogen storage box, a top cover is arranged at the opening of the freezing box, a controller is arranged on the top cover, a support plate is arranged inside the freezing box, the support plate is installed at the bottom of the freezing box, a cold source chamber is formed between the support plate and the bottom of the freezing box, liquid nitrogen is contained in the cold source chamber, and a second liquid level sensor is arranged in the cold source chamber. The support plate is provided with a fixing device for fixing the semen capillary tube through a first elastic member. The present invention uses the freezing device to freeze the frozen semen in the semen capillary tube, clamps the semen capillary tube in the freezing box through the fixing device, and can monitor the temperature inside the freezing box throughout the process through the controller to prevent the quality of the frozen semen from being affected when the temperature fluctuates during transportation.

Description

Technical Field

[0001] The present invention relates to the field of freezing technology, and specifically relates to a freezing device and a freezing method for high-throughput freezing of porcine semen. Background Art

[0002] With the development of biotechnology, artificial insemination and embryo transfer technologies are indispensable important links in the pig breeding industry and the utilization of pig germplasm resource preservation. Especially in China, as a large country of pig gene resources, the tasks of germplasm resource preservation and germplasm resource exchange among different countries and regions are becoming increasingly frequent and important. To increase the population size, genetic quality of livestock animals for animal husbandry or the meat quality of edible livestock animals for animal husbandry, artificial insemination of livestock animals is required. However, the semen of male animals prepared in advance must be frozen during transportation, otherwise, the quality of the semen of male animals (such as sperm motility) will be severely damaged, and the purpose of artificial insemination cannot be achieved.

[0003] Currently, in order to perform artificial insemination on livestock animals in remote pastoral areas, various large motor vehicles are often used to transport the semen of male animals to remote pastoral areas. Moreover, the large motor vehicles are equipped with generators and freezers, and the semen of male animals to be transported is frozen in the freezer. However, the freezer needs to be powered by the generator to work in order to keep the semen of male animals in a frozen state. That is, this technical measure of freezing the semen of male animals by means of an on-vehicle freezer and a generator has high power consumption, and the equipment cost and maintenance cost are relatively high. Summary of the Invention

[0004] Aiming at the above problems in the prior art, the present invention provides a freezing device and a freezing method for high-throughput freezing of porcine semen, and solves the problem that the existing freezing and transportation of porcine semen using large freezing trucks has relatively high consumption and maintenance costs.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] On the one hand, a freezing device for high-throughput freezing of porcine semen is provided, which includes a device body. The device body includes a freezing box and a liquid nitrogen storage box for storing liquid nitrogen that are interconnected; a first liquid level sensor is provided at the bottom inside the liquid nitrogen storage box, and a top cover is provided at the opening of the freezing box through a hinge. A control and monitoring system is provided on the top cover. The control and monitoring system includes a controller installed on the top cover. A display screen and control buttons are integrated on the controller. A support plate is provided inside the freezing box. The lower surface of the support plate is installed at the bottom of the freezing box through a telescopic rod. A cold source chamber is formed between the support plate and the bottom of the freezing box. Liquid nitrogen is contained in the cold source chamber, and a second liquid level sensor is provided at the bottom of the cold source chamber. A fixing device for fixing the semen capillary tube is provided on the upper surface of the support plate through a first elastic member. A pressure sensor and a temperature sensor are provided on the fixing device. The controller is respectively in signal connection with the first liquid level sensor, the second liquid level sensor, the pressure sensor, the temperature sensor and the telescopic rod.

[0007] In the present invention, a freezing device is used to freeze the frozen semen in the semen capillary tube. The semen capillary tube is clamped in the freezing box through the fixing device, and the controller can monitor the temperature inside the freezing box throughout the process and adjust it in a timely manner to prevent the quality of the frozen semen from being affected when the temperature fluctuates during transportation.

[0008] Furthermore, the fixing device includes a connecting plate, a fixing plate and a threaded rod. A bearing is installed on the connecting plate, a threaded hole is opened on the fixing plate, the threaded rod is installed on the bearing and passes through the threaded hole, and the connecting plate and the support plate are connected through a first elastic member.

[0009] In this solution, the fixing device includes a connecting plate, a fixing plate and a threaded rod. By adopting the above solution, the distance between the connecting plate and the fixing plate can be adjusted through the threaded rod to adapt to semen capillary tubes of different lengths.

[0010] Furthermore, a plurality of clamping holes are uniformly opened on the fixing plate, and a clamping component is provided in the clamping holes to clamp the semen capillary tube.

[0011] In this solution, a clamping component is provided in the clamping hole. By adopting the above solution, the clamping component can clamp the semen capillary tube in the clamping hole to prevent the semen capillary tube from colliding and being damaged when the device body shakes.

[0012] Furthermore, the clamping component includes two relatively arranged clamping channels. A fixing portion is provided at the bottommost part of the clamping channel. The fixing portion is connected to one end of a connecting rod through a second elastic member. The other end of the connecting rod is provided with a clamping plate. The two relatively arranged clamping plates clamp the semen capillary tube in the clamping hole, and the surface of the clamping plate that fits the semen capillary tube is an arc surface.

[0013] In this solution, the clamping assembly includes two relatively arranged clamping channels. With the above solution, the semen capillary tube presses against the second elastic member, causing the second elastic member to contract. The semen capillary tube falls into the clamping hole, and the second elastic member expands to push the arc surface of the clamping plate to abut against the semen capillary tube, thereby clamping the semen capillary tube in the clamping hole.

[0014] Furthermore, a plurality of limiting holes corresponding to the positions of the clamping holes are provided on the connecting plate. The semen capillary tubes are inserted into the corresponding limiting holes, and flexible buffer pads are arranged in the limiting holes.

[0015] In this solution, a plurality of limiting holes corresponding to the positions of the clamping holes are provided on the connecting plate. With the above solution, the limiting holes further limit the positions of the semen capillary tubes to prevent the semen capillary tubes from shaking, and the flexible buffer pads can protect the semen capillary tubes to prevent damage to the semen capillary tubes.

[0016] Furthermore, a measuring column is arranged at the central position of the lower surface of the fixing plate, and a pressure sensor and a temperature sensor are arranged on the measuring column.

[0017] In this solution, a pressure sensor and a temperature sensor are arranged on the measuring column. With the above solution, the pressure sensor and the temperature sensor can collect the atmospheric pressure and temperature in the freezer in real time and feed them back to the controller in real time to prevent excessive nitrogen production from liquid nitrogen, increasing the atmospheric pressure in the freezer, and the temperature drop affecting the quality of frozen semen.

[0018] Furthermore, a solenoid valve is arranged at the connection between the freezer and the liquid nitrogen storage tank, an exhaust channel is reserved on the top cover, and a pressure relief exhaust valve is arranged in the exhaust channel. Both the solenoid valve and the pressure relief exhaust valve are connected to the controller by signal.

[0019] In this solution, the controller receives the information from the pressure sensor, liquid level sensor and temperature sensor. When too much liquid nitrogen in the freezer evaporates, the controller opens the solenoid valve to send the reserved liquid nitrogen in the liquid nitrogen storage tank into the freezer, and the controller opens the pressure relief exhaust valve to discharge the nitrogen in the freezer to reduce the pressure in the freezer.

[0020] Furthermore, elastic buffer pads that fit the inner wall of the freezer are arranged on the circumferences of the connecting plate and the fixing plate, and a sealing pad that fits the inner wall of the freezer is arranged on the circumference of the support plate.

[0021] In this solution, the elastic buffer pads can buffer the acting force and protect the semen capillary tubes when the device body collides or shakes.

[0022] Furthermore, a number of through holes are provided on the connecting plate, the fixing plate and the support plate.

[0023] This solution uses the liquid nitrogen fumigation method to freeze the semen capillary tubes, and the through holes facilitate the passage of nitrogen.

[0024] On the other hand, a freezing method for a freezing device using high-throughput frozen pig semen is characterized by including the following steps:

[0025] Step S1: Clean the fixing device, the support plate, the telescopic rod, and the inner wall of the freezing box;

[0026] Step S2: Detect whether the connection of the controller is normal. If it is normal, slowly pour liquid nitrogen into the liquid nitrogen storage tank;

[0027] Step S3: Start the controller, control the solenoid valve to open, and the liquid nitrogen in the liquid nitrogen storage tank flows into the cold source chamber for precooling for 20 to 30 minutes;

[0028] Step S4: After the precooling is completed, cover the top cover. The controller collects the liquid level information, the temperature information, and the pressure information in the cold source chamber, and adjusts the telescopic rod, the solenoid valve, and the pressure relief and exhaust valve based on this information through the controller;

[0029] Step S5: After the temperature stabilizes, quickly place the semen straw containing frozen semen in the fixing device;

[0030] Step S6: After adding liquid nitrogen to the liquid nitrogen storage tank again, it can be transported. And the controller continuously receives the temperature information and the pressure information in the freezing box and displays them on the display screen.

[0031] The present invention discloses a freezing device and a freezing method for high-throughput frozen pig semen, and its beneficial effects are as follows:

[0032] 1. The present invention uses a freezing device to freeze the frozen semen in the semen straw, with a simple structure, safe operation, and high stability, ensuring the safety of the semen, significantly reducing the equipment cost, and also greatly saving the maintenance cost. It can fully meet the needs of pig semen freezing. The internal temperature of the freezing box can be monitored throughout the process through the controller and adjusted in a timely manner to prevent the quality of the frozen semen in the semen straw from being affected when the temperature fluctuates during transportation.

[0033] 2. The fixing device in the device body of the present invention adopts a structure in which a clamping component is matched with a limiting hole to fix the semen straw and prevent the semen straw from shaking. Elastic buffer pads are arranged on the circumferences of the connecting plate and the fixing plate, which can buffer the acting force during collision or shaking and further protect the semen straw.

[0034] 3. The device body of the present invention is provided with a liquid nitrogen storage tank communicated with the freezing box through a solenoid valve. The liquid nitrogen storage tank is used to store liquid nitrogen for timely adding liquid nitrogen during transportation preparation.

[0035] 4. The device body of the present invention is provided with a control and monitoring system. The controller in the control and monitoring system receives the atmospheric pressure information, temperature information, and liquid level information inside the freezer to control the telescopic rod, solenoid valve, and pressure relief and exhaust valve, and timely adjust the temperature and atmospheric pressure inside the freezer to prevent affecting the quality of the frozen semen in the semen straw. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic structural diagram of a freezing device for high-throughput freezing of porcine semen according to the present invention.

[0037] Figure 2 It is a schematic cross-sectional view of a freezing device for high-throughput freezing of porcine semen according to the present invention.

[0038] Figure 3 It is a schematic structural diagram of the fixing device according to the present invention.

[0039] Figure 4 It is a top view schematic diagram of the fixing device according to the present invention.

[0040] Figure 5 For the present invention Figure 3 The structural schematic diagram at position A.

[0041] Among them, 1. Device body; 2. Freezer; 3. Liquid nitrogen storage tank; 4. Top cover; 5. Controller; 6. Pressure relief and exhaust valve; 7. Fixing device; 71. Connecting plate; 711. Bearing; 712. Limiting hole; 713. Flexible buffer pad; 72. Fixed plate; 721. Clamping hole; 722. Threaded hole; 73. Threaded rod; 74. Semen straw; 75. Clamping assembly; 751. Clamping channel; 752. Fixed part; 753. Second elastic member; 754. Connecting rod; 755. Clamping plate; 76. Measuring column; 761. Pressure sensor; 762. Temperature sensor; 8. Telescopic rod; 9. Support plate; 10. Cold source bin; 11. Second liquid level sensor; 12. Solenoid valve; 13. First liquid level sensor; 14. First elastic member; 15. Sealing gasket; 16. Elastic buffer pad. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0042] The specific embodiments of the present invention are described to facilitate those skilled in the art of the present technology to understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those of ordinary skill in the art of the present technology, as long as various changes are within the spirit and scope of the present invention defined and determined by the appended claims, these changes are obvious, and all inventions and creations using the concept of the present invention are within the scope of protection.

[0043] Embodiment 1

[0044] Refer to Figure 1 and Figure 2, which is a schematic structural diagram of a device for high-throughput rapid freezing of pig semen in this embodiment, aims to solve the problem that the existing frozen semen transportation of pig semen uses large freezing trucks, resulting in relatively high consumption and maintenance costs. The following will describe the specific structure in this embodiment in detail.

[0045] A freezing device for high-throughput freezing of pig semen, which includes a device body 1. The device body 1 includes a freezing box 2 and a liquid nitrogen storage box 3 for storing liquid nitrogen that are interconnected.

[0046] Specifically, a top cover 4 is provided at the opening of the freezing box 2 through a hinge. A control and monitoring system is provided on the top cover 4. The control and monitoring system includes a controller 5 installed on the top cover 4. A display screen and control buttons are integrated on the controller 5.

[0047] In this embodiment, the controller 5 in the control and monitoring system is used to monitor and collect the working environment information inside the freezing box 2, including temperature, liquid level height, and atmospheric pressure, and adjust the working environment inside the freezing box 2 according to the information to make the freezing box 2 generate a suitable temperature.

[0048] A support plate 9 is provided inside the freezing box 2. The lower surface of the support plate 9 is installed at the bottom of the freezing box 2 through a telescopic rod 8. A cold source chamber 10 is formed between the support plate 9 and the bottom of the freezing box 2. Liquid nitrogen is contained in the cold source chamber 10, and a second liquid level sensor 11 is provided at the bottom of the cold source chamber 10. A first liquid level sensor 13 is provided at the bottom of the liquid nitrogen storage box 3.

[0049] In this embodiment, a total of 4 groups of telescopic rods 8 are provided, which are respectively located at the four corner positions of the support plate 9. The telescopic rod 8 adopts an existing micro electric push rod, and its specific stroke is 100 mm. The controller 5 can control the telescopic rod 8 to expand and contract, thereby reducing the height of the support plate 9 and shortening the distance between the semen capillary tube 74 and the liquid nitrogen, so as to adjust the temperature at the position where the semen capillary tube 74 is located.

[0050] A fixing device 7 for fixing the semen capillary tube 74 is provided on the upper surface of the support plate 9 through a first elastic member 14. A pressure sensor 761 and a temperature sensor 762 are provided on the fixing device 7.

[0051] In this embodiment, the first elastic member 14 can be a spring. When the device body 1 collides or shakes, the spring buffers the acting force to protect the semen capillary tube 74. The pressure sensor 761 and the temperature sensor 762 collect the temperature and pressure information at the fixing device 7 and feedback it to the controller 5.

[0052] The controller 5 is respectively connected to the first liquid level sensor 13, the second liquid level sensor 11, the pressure sensor 761, the temperature sensor 762 and the telescopic rod 8 in a signal connection. An electromagnetic valve 12 is provided at the connection between the freezer 2 and the liquid nitrogen storage tank 3. An exhaust passage is reserved on the top cover 4, and a pressure relief exhaust valve 6 is arranged in the exhaust passage. Both the electromagnetic valve 12 and the pressure relief exhaust valve 6 are connected to the controller 5 in a signal connection.

[0053] In this embodiment, the controller 5 uses an existing single-chip microcomputer, and its specific model is the STC89C52 single-chip microcomputer. A display screen and control buttons are integrated on the single-chip microcomputer. The controller 5 displays the received information on the display screen, and the staff can operate the controller 5 through the control buttons. The controller 5 can be self-powered or connected to an external power supply, and the specific connection method can be selected according to actual needs.

[0054] It should be noted that the specific model specifications of the first liquid level sensor 13, the second liquid level sensor 11, the pressure sensor 761, the temperature sensor 762, the electromagnetic valve 12 and the pressure relief exhaust valve 6 need to be selected and determined according to the actual specifications of the freezing device, etc. The specific selection calculation method adopts the existing technology in this field, so it will not be elaborated in detail.

[0055] The pressure sensor 761 and the temperature sensor 762 can collect the atmospheric pressure and temperature in the freezer 2 in real time and feedback them to the controller 5 in real time, preventing excessive nitrogen generated by liquid nitrogen, increasing the atmospheric pressure in the freezer, and the temperature decrease affecting the quality of frozen semen. Excessive pressure also poses a safety hazard.

[0056] When the controller 5 receives the information of the first liquid level sensor 13, the second liquid level sensor 11 and the temperature sensor 762, if the temperature information received by the controller 5 is too low, the controller 5 controls the telescopic rod 8 to expand and contract, thereby reducing the height of the support plate 9 and narrowing the distance between the semen capillary 74 and the liquid nitrogen, so as to adjust the temperature of the position where the semen capillary 74 is located.

[0057] When the controller 5 receives the information of the second liquid level sensor 11, if the controller 5 receives the liquid level information collected by the second liquid level sensor 11 in the cold source bin 10 decreases, the controller 5 opens the electromagnetic valve 12 to send the reserved liquid nitrogen in the liquid nitrogen storage tank 3 into the freezer 2.

[0058] When the controller 5 receives the information fed back by the pressure sensor 761 and the temperature sensor 762, if the pressure information received by the controller 5 is too high and the temperature is too low, it is considered that too much liquid nitrogen in the freezer 2 has evaporated. The controller 5 opens the pressure relief exhaust valve 6 to discharge the nitrogen in the freezer 2 and reduce the nitrogen pressure in the freezer 2.

[0059] The present invention uses a freezing device to freeze the frozen semen in the semen straw, which has a simple structure, safe operation, high stability, ensures the safety of the semen, significantly reduces the equipment cost, and also greatly saves the maintenance cost. It can fully meet the needs of pig semen freezing, and the internal temperature of the freezer can be monitored throughout the process through the control and detection system. When there is a change, the controller 5 can adjust in time to prevent the quality of the frozen semen in the semen straw from being affected when the temperature fluctuates during transportation.

[0060] Embodiment 2

[0061] Reference Figure 1 and Figure 2 , which is a schematic structural diagram of the fixing device and the clamping device in this embodiment, aims to clamp and fix the semen straw, and this embodiment gives a further solution for the fixing device and the clamping device.

[0062] The fixing device 7 includes a connecting plate 71, a fixing plate 72 and a threaded rod 73.

[0063] Specifically, a bearing 711 is installed on the connecting plate 71, a threaded hole 722 is opened on the fixing plate 72, the threaded rod 73 is installed on the bearing 711 and passes through the threaded hole 722, and the connecting plate 71 and the support plate 9 are connected by a first elastic member 14.

[0064] In this embodiment, there are 4 threaded holes 722, threaded rods 73 and bearings 711. The 4 threaded holes 722 and bearings 711 are respectively located at the four corners of the fixing plate 72 and the connecting plate 71. The threaded rod 73 is installed on the bearing 711 and is threadedly connected to the threaded hole 722. The distance between the connecting plate 71 and the fixing plate 72 is adjusted by the threaded rod 73 to adapt to semen straws 74 of different lengths.

[0065] A plurality of clamping holes 721 are evenly opened on the fixing plate 72, and a clamping assembly 75 is arranged in the clamping holes 721. The clamping assembly 75 clamps the semen straw 74. The clamping assembly 75 can clamp the semen straw 74 in the clamping hole 721 to prevent the semen straw 74 from colliding when the device body 1 shakes, damaging the semen straw.

[0066] The clamping assembly 75 includes two relatively arranged clamping channels 751. A fixing part 752 is arranged at the bottom of the clamping channel 751. The fixing part 752 is connected to one end of a connecting rod 754 through a second elastic member 753. The other end of the connecting rod 754 is provided with a clamping plate 755. The two relatively arranged clamping plates 755 clamp the semen straw 74 in the clamping hole 721, and the surface of the clamping plate 755 that fits the semen straw 74 is an arc surface.

[0067] In this embodiment, when the semen capillary tube 74 is inserted into the clamping hole 721, the semen capillary tube 74 presses against the second elastic member 753 to contract the second elastic member 753. After the semen capillary tube 74 falls into the clamping hole 721, the second elastic member 753 extends to push the arc surface of the clamping plate 755 to abut against the semen capillary tube 74, thereby clamping the semen capillary tube 74 in the clamping hole 721. The second elastic member 753 in this embodiment can be a spring.

[0068] A plurality of limiting holes 712 corresponding to the positions of the clamping holes 721 are formed in the connecting plate 71. The semen capillary tube 74 is inserted into the corresponding limiting holes 712, and a flexible buffer pad 713 is arranged in the limiting holes 712.

[0069] In this embodiment, the limiting holes 712 on the connecting plate 71 further limit the position of the semen capillary tube 74 to prevent the semen capillary tube 74 from shaking. The flexible buffer pad 713 can protect the semen capillary tube 74 to prevent damage to the semen capillary tube 74.

[0070] A plurality of through holes are formed in the connecting plate 71, the fixing plate 72, and the support plate 9. When the semen capillary tube 74 is frozen by the liquid nitrogen fumigation method, the through holes can facilitate the passage of nitrogen.

[0071] Elastic buffer pads 16 that fit the inner wall of the freezing box 2 are arranged on the circumferences of the connecting plate 71 and the fixing plate 72, and a sealing pad 15 that fits the inner wall of the freezing box 2 is arranged on the circumference of the support plate 9. In this embodiment, the elastic buffer pads 16 can buffer the acting force and protect the semen capillary tube 74 when the device body 1 collides or shakes.

[0072] The control and monitoring system further includes a measuring column 76 provided at the central position of the lower surface of the fixing plate 72, and a pressure sensor 761 and a temperature sensor 762 are arranged on the measuring column 76.

[0073] Embodiment 3

[0074] This embodiment provides a freezing method for a freezing device for high-throughput freezing of porcine semen, which includes the following steps:

[0075] Step S1: Clean the fixing device 7, the support plate 9, the telescopic rod 8, and the inner wall of the freezing box 2.

[0076] Step S2: Detect whether the connection of the controller 5 is normal. If it is normal, slowly pour liquid nitrogen into the liquid nitrogen storage tank 3.

[0077] Step S3: Start the controller 5, control the solenoid valve 12 to open, and the liquid nitrogen in the liquid nitrogen storage tank 3 flows into the cold source bin 10 for precooling for 20 to 30 minutes;

[0078] Step S4: After the precooling ends, cover the top cover 4. The controller 5 collects the liquid level information in the cold source bin 10, the temperature information and the pressure information in the freezer 2, and controls the telescopic rod 8, the solenoid valve 12 and the pressure relief and exhaust valve 6 for adjustment based on this information;

[0079] Specifically, in step S4:

[0080] When the controller 5 receives the information from the first liquid level sensor 13, the second liquid level sensor 11 and the temperature sensor 762, if the temperature information received by the controller 5 is too low, the controller 5 controls the telescopic rod 8 to expand and contract, thereby reducing the height of the support plate 9 and narrowing the distance between the semen capillary 74 and the liquid nitrogen, so as to adjust the temperature of the position where the semen capillary 74 is located.

[0081] The controller 5 receives the information from the second liquid level sensor 11. If the controller 5 receives the liquid level information in the cold source bin 10 collected by the second liquid level sensor 11 and it decreases, the controller 5 opens the solenoid valve 12 to send the reserve liquid nitrogen in the liquid nitrogen storage tank 3 into the freezer 2.

[0082] The controller 5 receives the information fed back by the pressure sensor 761 and the temperature sensor 762. If the pressure information received by the controller 5 is too large and the temperature is too low, it is considered that too much liquid nitrogen in the freezer 2 has evaporated. The controller 5 opens the pressure relief and exhaust valve 6 to discharge the nitrogen in the freezer 2, reducing the nitrogen pressure in the freezer 2.

[0083] Step S5: After the temperature stabilizes, quickly place the semen capillary 74 containing frozen semen in the fixing device 7;

[0084] Step S6: After adding liquid nitrogen to the liquid nitrogen storage tank 3 again, it can be transported, and the controller 5 continuously receives the temperature information and the pressure information in the freezer 2 and displays them on the display screen.

[0085] Although the specific implementation manners of the invention have been described in detail with reference to the accompanying drawings, it should not be construed as a limitation on the protection scope of this patent. Within the scope described in the claims, various modifications and deformations that can be made by those skilled in the art without creative work still fall within the protection scope of this patent.

Claims

1. A freezing device for high-throughput freezing of porcine semen, characterized in that: It includes a device body (1); The device body (1) includes a freezer (2) and a liquid nitrogen storage tank (3) for storing liquid nitrogen that are connected to each other; a first liquid level sensor (13) is provided at the bottom inside the liquid nitrogen storage tank (3); A top cover (4) is provided at the opening of the freezer (2) through a hinge, and a control and monitoring system is provided on the top cover (4); The control and monitoring system includes a controller (5) installed on the top cover (4), and a display screen and control buttons are integrated on the controller (5); A support plate (9) is provided inside the freezer (2), the lower surface of the support plate (9) is installed at the bottom of the freezer (2) through a telescopic rod (8), a cold source chamber (10) is formed between the support plate (9) and the bottom of the freezer (2), liquid nitrogen is placed in the cold source chamber (10), and a second liquid level sensor (11) is provided at the bottom of the cold source chamber (10); A fixing device (7) for fixing a semen capillary tube (74) is provided on the upper surface of the support plate (9) through a first elastic member (14), and a pressure sensor (761) and a temperature sensor (762) are provided on the fixing device (7); The controller (5) is respectively in signal connection with the first liquid level sensor (13), the second liquid level sensor (11), the pressure sensor (761), the temperature sensor (762), and the telescopic rod (8); The fixing device (7) includes a connecting plate (71), a fixing plate (72), and a threaded rod (73). A bearing (711) is installed on the connecting plate (71), a threaded hole (722) is provided on the fixing plate (72), the threaded rod (73) is installed on the bearing (711) and passes through the threaded hole (722); the connecting plate (71) is connected to the support plate (9) through a first elastic member (14); A plurality of clamping holes (721) are evenly provided on the fixing plate (72), a clamping assembly (75) is provided in the clamping holes (721), and the clamping assembly (75) clamps the semen capillary tube (74); A measuring column (76) is provided at the center position of the lower surface of the fixing plate (72), and a pressure sensor (761) and a temperature sensor (762) are provided on the measuring column (76).

2. The cryogenic device for high-throughput cryopreservation of porcine semen according to claim 1, wherein: The clamping assembly (75) includes two relatively arranged clamping channels (751), a fixing portion (752) is provided at the bottommost part of the clamping channels (751), the fixing portion (752) is connected to one end of a connecting rod (754) through a second elastic member (753), the other end of the connecting rod (754) is provided with a clamping plate (755), and two relatively arranged clamping plates (755) clamp the semen capillary tube (74) in the clamping hole (721), and the surface of the clamping plate (755) that fits the semen capillary tube (74) is an arc surface.

3. The cryogenic device for high-throughput cryopreservation of porcine semen according to claim 1, wherein: A plurality of limiting holes (712) corresponding to the positions of the clamping holes (721) are provided on the connecting plate (71), the semen capillary tube (74) is inserted into the corresponding limiting holes (712), and a flexible buffer pad (713) is provided in the limiting holes (712).

4. The cryogenic device for high-throughput cryopreservation of porcine semen according to claim 1, wherein: An electromagnetic valve (12) is provided at the connection between the freezer (2) and the liquid nitrogen storage tank (3). An exhaust passage is reserved on the top cover (4), and a pressure relief exhaust valve (6) is arranged in the exhaust passage. Both the electromagnetic valve (12) and the pressure relief exhaust valve (6) are signal-connected to the controller (5).

5. The cryogenic device for high-throughput cryopreservation of porcine semen according to claim 1, characterized in that: Elastic buffer pads (16) that fit the inner wall of the freezer (2) are provided on the circumferences of the connecting plate (71) and the fixing plate (72); a sealing pad (15) that fits the inner wall of the freezer (2) is provided on the circumference of the support plate (9).

6. The cryogenic device for high-throughput cryopreservation of porcine semen according to claim 1, characterized in that: A number of through holes are provided on the connecting plate (71), the fixing plate (72) and the support plate (9).

7. A freezing method using the freezing device for high-throughput frozen porcine semen according to any one of claims 1-6, characterized in that, It includes the following steps: Step S1: Clean the fixing device (7), the support plate (9), the telescopic rod (8) and the inner wall of the freezer (2); Step S2: Detect whether the connection of the controller (5) is normal. If it is normal, slowly pour liquid nitrogen into the liquid nitrogen storage tank (3); Step S3: Start the controller (5), control the electromagnetic valve (12) to open, and the liquid nitrogen in the liquid nitrogen storage tank (3) flows into the cold source bin (10) for precooling for 20 to 30 minutes; Step S4: After the precooling is completed, cover the top cover (4). The controller (5) collects the liquid level information in the cold source bin (10), the temperature information and the pressure information in the freezer (2), and controls the telescopic rod (8), the electromagnetic valve (12) and the pressure relief exhaust valve (6) to make adjustments based on this information through the controller (5); Step S5: After the temperature stabilizes, quickly place the semen straw (74) containing frozen sperm in the fixing device (7); Step S6: After adding liquid nitrogen to the liquid nitrogen storage tank (3) again, it can be transported, and the controller (5) receives the temperature information and the pressure information in the freezer (2) in real time and displays them on the display screen.

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