Fluidic system for a gene sequencer

Abstract

The disclosure provides a fluid system of a gene sequencer, which comprises a valve group, a gang pump, a waste liquid module, a pipeline and a plurality of chip modules; each group of the chip modules is connected to the valve group through the pipeline, and the gang pump is connected to the valve group and the waste liquid module through the pipeline; the valve group comprises a plurality of electromagnetic valves, and the valve group is used for determining a current chip module in a working state in the plurality of chip modules by a valve position of each electromagnetic valve; the current chip module is used for receiving a reagent, performing a reagent reaction based on the reagent, generating reagent reaction waste liquid, and delivering the reagent reaction waste liquid to the gang pump through the valve group; and the gang pump is used for delivering the reagent reaction waste liquid to the waste liquid module. In the disclosure, the gene sequencer comprising the fluid system has the characteristics of miniaturization, high throughput and chip free combination on machine.

Description

Technical Field

[0001] This disclosure relates to the field of gene sequencer technology, and more specifically, to a fluid system for a gene sequencer. Background Technology

[0002] A gene sequencer, also known as a deoxyribonucleic acid (DNA) sequencer, is an instrument used to determine the base sequence, type, and quantification of DNA fragments. It is commonly used in human genome sequencing, gene diagnosis of human genetic diseases, infectious diseases, and cancer, forensic paternity testing and individual identification, screening of bioengineering drugs, and hybridization breeding of plants and animals.

[0003] With the diversification of business needs for gene sequencers, the development trend of gene sequencers is towards low cost, miniaturization, high throughput, and flexible chip combination. Therefore, it is particularly important to propose a gene sequencer that meets the above requirements. Summary of the Invention

[0004] In view of this, the present disclosure provides at least one fluid system for a gene sequencer.

[0005] In a first aspect, this disclosure provides a fluid system for a gene sequencer, comprising: a valve group, a manifold pump, a waste liquid module, pipes, and multiple sets of chip modules; wherein each set of chip modules is connected to the valve group via the pipes, and the manifold pump is connected to the valve group and the waste liquid module via the pipes;

[0006] The valve group includes multiple solenoid valves, and the valve group is used to determine the current chip module in the working state among the multiple chip modules by using the valve position of each solenoid valve. The current chip module is used to receive reagents, perform reagent reactions based on the reagents, generate reagent reaction waste liquid, and transport the reagent reaction waste liquid to the combined pump through the valve group. The combined pump is used to transport the reagent reaction waste liquid to the waste liquid module.

[0007] In one possible implementation, it further includes: a support module and a rotary valve; the support module is provided with multiple reagent kits and a reagent needle corresponding to each reagent kit; each reagent needle is connected to one port of the rotary valve; the rotary valve is connected to the multiple sets of chip modules; The reagent needle is used to insert into the reagent port on the reagent kit to draw reagent from the reagent kit and deliver the drawn reagent to the current chip module through the port of the connected rotary valve.

[0008] In one possible implementation, when each group of chip modules is connected to the valve group through multiple pipes, the valve group is connected to the manifold pump through multiple pipes; the manifold pump includes multiple syringes; wherein the number of pipes between the chip module and the valve group is the same as the number of pipes between the valve group and the manifold pump, and the number of pipes between the valve group and the manifold pump is the same as the number of syringes; The syringe is used to control the current chip module to deliver the reagent reaction waste liquid to the valve group through the pipe corresponding to the syringe by pulling the piston on the syringe; The valve assembly is used to deliver the reagent reaction waste liquid to the syringe of the combined pump through a pipe corresponding to the syringe; The syringe is also used to push the reagent reaction waste liquid to the waste liquid module by pushing the piston on the syringe.

[0009] In one possible implementation, it further includes: a cleaning module; the cleaning module is connected to the combined pump via a pipeline; The cleaning module is used to deliver the stored cleaning reagent to the combined pump; The combined pump is used to deliver the cleaning reagent into the syringe and, by pushing the piston of the syringe, deliver the cleaning reagent to the waste liquid module.

[0010] In one possible implementation, the valve group is further configured to determine the chip module to be cleaned among the multiple sets of chip modules based on the valve position of each of the solenoid valves. After receiving the cleaning reagent, the combined pump is further configured to: input the cleaning reagent to the chip module to be cleaned through the valve group.

[0011] In one possible implementation, when the system includes a support module and a rotary valve, the chip module to be cleaned, upon receiving the cleaning reagent, is further configured to deliver the cleaning reagent to the reagent kit of the support module through the rotary valve.

[0012] In one possible implementation, the chip module includes at least a liquid inlet, a liquid outlet, and a temperature control module; The chip module, when receiving reagents, performing a reagent reaction based on the reagents to generate reagent reaction waste liquid, and conveying the reagent reaction waste liquid to the combined pump through the valve group, is used for: The reagent is received from the inlet; the reagent reaction temperature is controlled by the temperature control module; the reagent reaction is carried out based on the reagent at the reagent reaction temperature to generate reagent reaction waste liquid, and the reagent reaction waste liquid is transported from the outlet to the valve group, and the reagent reaction waste liquid is transported to the combined pump through the valve group.

[0013] In one possible implementation, the solenoid valve is a two-position three-way solenoid valve; each solenoid valve includes a first connection port, a second connection port, and a third connection port; the solenoid valve includes a first valve position indicating energization and a second valve position indicating de-energization; The multiple chip modules are respectively connected to the first or second port of the solenoid valve; the third port of the solenoid valve is connected to the combined pump.

[0014] This disclosure provides a fluid system for a gene sequencer. The system includes multiple sets of chip modules, each with multiple channels for discharging reagent reaction waste liquid, thus increasing the gene sequencer's throughput and enabling it to meet high-throughput requirements. The chip modules are connected to a valve group containing multiple solenoid valves. The valve position of each solenoid valve determines the currently active chip module among the multiple sets, enabling flexible chip combination and improving the gene sequencer's operational flexibility.

[0015] Meanwhile, the fluid system can control one chip module to be in working state at a time through the valve group, realizing individual sample injection for each group of chip modules. This makes the valve group setting simpler and more streamlined, thus enabling the gene sequencer to have the characteristics of low cost and miniaturization.

[0016] To make the above-mentioned objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the accompanying drawings used in the embodiments will be briefly described below. These drawings are incorporated in and constitute a part of this specification. They illustrate embodiments conforming to this disclosure and, together with the specification, serve to explain the technical solutions of this disclosure. It should be understood that the following drawings only show some embodiments of this disclosure and should not be considered as limiting the scope. Those skilled in the art can obtain other related drawings based on these drawings without creative effort.

[0018] Figure 1 A schematic diagram of the fluid system architecture of a gene sequencer provided in an embodiment of this disclosure is shown; Figure 2aThis illustration shows a schematic diagram of the structure of a solenoid valve in a fluid system of a gene sequencer provided in an embodiment of this disclosure; Figure 2b This illustration shows a schematic diagram of the connection relationship between the valve group and the chip module in a fluid system of a gene sequencer provided in an embodiment of this disclosure; Figure 3 A schematic diagram of the fluid system architecture of another gene sequencer provided in an embodiment of this disclosure is shown; Figure 4 This illustration shows a schematic diagram of the connection relationship between the valve group and the chip module in a fluid system of a gene sequencer provided in an embodiment of this disclosure; Figure 5 A schematic diagram of the fluid system architecture of another gene sequencer provided in an embodiment of this disclosure is shown. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this disclosure, and not all embodiments. The components of the embodiments of this disclosure described and shown in the accompanying drawings can generally be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this disclosure provided in the accompanying drawings is not intended to limit the scope of the claimed disclosure, but merely represents selected embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the embodiments of this disclosure without inventive effort are within the scope of protection of this disclosure.

[0020] With the diversification of business demands for gene sequencers, the development trend of gene sequencers is towards lower cost, miniaturization, high throughput, and flexible chip combination. Based on this, this disclosure provides a fluid system for a gene sequencer. This fluid system incorporates multiple sets of chip modules, increasing the throughput of the gene sequencer and enabling it to meet high-throughput requirements. The chip modules are connected to a valve group, which includes multiple solenoid valves. The valve position of each solenoid valve determines the currently active chip module among the multiple sets, enabling flexible chip combination and improving the flexibility of the gene sequencer.

[0021] Meanwhile, the fluid system can control one chip module to be in working state at a time through the valve group, realizing individual sample injection for each group of chip modules. This makes the valve group setting simpler and more streamlined, thus enabling the gene sequencer to have the characteristics of low cost and miniaturization.

[0022] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0023] To facilitate understanding of the embodiments of this disclosure, a detailed description of the fluid system of a gene sequencer disclosed in this disclosure is provided. See also: Figure 1 The diagram shows the architecture of the fluid system of a gene sequencer provided in this embodiment of the present disclosure. The fluid system includes: a valve group 500, a manifold pump 600, a waste liquid module 700, a pipeline 300, and multiple sets of chip modules 400. Each set of chip modules 400 is connected to the valve group 500 through the pipeline 300, and the manifold pump 600 is connected to the valve group 500 and the waste liquid module 700 through the pipeline 300.

[0024] The valve group includes multiple solenoid valves, and the valve group is used to determine the current chip module in the working state among the multiple sets of chip modules by using the valve position of each solenoid valve.

[0025] The current chip module is used to receive reagents, perform reagent reactions based on the reagents to generate reagent reaction waste liquid, and transport the reagent reaction waste liquid to the combined pump through the valve group.

[0026] The combined pump is used to transport the reagent reaction waste liquid to the waste liquid module.

[0027] In implementation, the current operating chip module among multiple chip modules can be determined by adjusting the valve positions of multiple solenoid valves within the valve assembly. The solenoid valve positions include a first position indicating energization and a second position indicating de-energization. The relationship between the current chip module and the valve positions of the multiple solenoid valves can be determined based on the connection relationships between each group of chip modules and the valve assembly.

[0028] In one embodiment, the solenoid valve is a two-position three-way solenoid valve; each solenoid valve includes a first connection port, a second connection port, and a third connection port; the solenoid valve includes a first valve position indicating energization and a second valve position indicating de-energization; the multiple chip modules are respectively connected to the first connection port or the second connection port of the solenoid valve; the third port of the solenoid valve is connected to the manifold pump.

[0029] See Figure 2a The schematic diagram of the solenoid valve shown includes a first connection port 21, a second connection port 22, and a third connection port 23. The chip module is connected to the first or second connection port of each solenoid valve in the valve group, and the third connection port of the solenoid valve is connected to the manifold pump.

[0030] For example, see Figure 2bThe diagram shows the connection relationship between the valve group and the chip module. If there are 4 groups of chip modules, namely chip module 1, chip module 2, chip module 3, and chip module 4, the valve group can include 3 solenoid valves: solenoid valve 1, solenoid valve 2, and solenoid valve 3. Chip module 1 is connected to the first connection port of solenoid valve 1, chip module 2 is connected to the second connection port of solenoid valve 1, chip module 3 is connected to the first connection port of solenoid valve 2, chip module 4 is connected to the second connection port of solenoid valve 2, the third connection port of solenoid valve 1 is connected to the first connection port of solenoid valve 3, the third connection port of solenoid valve 2 is connected to the second connection port of solenoid valve 3, and the third connection port of solenoid valve 3 is connected to the combined pump.

[0031] See Table 1 below for the mapping relationship between the valve position of the solenoid valve and the working state of the chip module.

[0032] Table 1. Mapping relationship between solenoid valve position and chip module operating state

[0033] As shown in the table above, chip module 1 is in working state when solenoid valve 1 is in the first position indicating energization (G), solenoid valve 2 is in the second position indicating de-energization (T), and solenoid valve 3 is in the first position indicating energization. Chip module 2 is in working state when solenoid valve 1 is in the second position indicating de-energization, solenoid valve 2 is in the second position indicating de-energization, and solenoid valve 3 is in the first position indicating energization. Therefore, the working state of the chip module is controlled by the valve positions of each solenoid valve in the valve group.

[0034] In practice, the number of solenoid valves in the valve group and the connection relationship between the solenoid valves and the chip module can be set as needed; this is only an example.

[0035] After the valve assembly uses the valve positions of each solenoid valve to determine the currently active chip module among multiple chip modules, the current chip module receives reagents. These reagents can be any reagents required for the gene sequencing process, without specific limitations here. The reagents received by the current chip module can be supplied from the reagent kit or input into the current chip module in response to manual operation.

[0036] The current chip module performs a reagent reaction based on the received reagents, generating reagent reaction waste liquid. This reagent reaction can be any reaction process present in gene sequencing. The current chip module delivers the reagent reaction waste liquid to a valve assembly, which then delivers it to a manifold pump. The manifold pump then delivers the reagent reaction waste liquid to a waste liquid module. This waste liquid module can be, for example, a waste liquid container used to store the reagent reaction waste liquid.

[0037] In practice, the operation of a manifold pump can drive the flow of reagents and reagent reaction waste liquid in the fluid system. For example, the manifold pump can move its built-in piston to allow reagents to flow through the current chip module, and after generating reagent reaction waste liquid, deliver the waste liquid to the valve assembly, and then into the manifold pump. The manifold pump then controls the movement of the piston again to deliver the inflowing reagent reaction waste liquid to the waste liquid module.

[0038] The fluid system includes piping for transporting reagents and reagent reaction waste. Each chip module is connected to a valve assembly via piping, the combined pump is connected to the valve assembly via piping, and the combined pump is connected to the waste module via piping. This piping can be used to transport any liquid present in the fluid system, such as reagents drawn into the system or reagent reaction waste generated.

[0039] For example, reagents can be piped into the current chip module, and after the current chip module generates reagent reaction waste liquid, the reagent reaction waste liquid is piped into the valve group. The valve group then pipes the reagent reaction waste liquid to the manifold pump. The manifold pump then pipes the reagent reaction waste liquid to the waste liquid module.

[0040] In one alternative implementation, see Figure 3 As shown, the fluid system may further include: a support module 100 and a rotary valve 200; the support module 100 is provided with a plurality of reagent kits and a reagent needle corresponding to each reagent kit; each of the reagent needles is connected to one port of the rotary valve 200; the rotary valve 200 is connected to the plurality of chip modules 400.

[0041] The reagent needle is used to insert into the reagent port on the reagent kit to draw reagent from the reagent kit and deliver the drawn reagent to the current chip module through the port of the connected rotary valve.

[0042] The scaffold module contains multiple reagent kits for storing reagents required for the gene sequencing process. Each kit has a reagent needle, and each needle is connected to one port of a rotary valve. The number of ports on the rotary valve is no less than the number of kits in the scaffold module. The reagent needle is inserted into the reagent port on the kit to draw reagents from the kit. The drawn reagents are then fed into the connected port of the rotary valve, and finally, the reagents are fed into the current chip module through the common port of the rotary valve.

[0043] The support module is connected to the rotary valve via pipes, and the rotary valve is connected to each chip module via pipes. During implementation, after the reagent needle on the support module draws up the reagent, it delivers the reagent through pipes to the rotary valve, which then transports the reagent through pipes to the current chip module.

[0044] Here, by setting multiple reagent kits on the scaffold module, each reagent kit is connected to one port of the rotary valve. The reagent kit can store various types of reagents, making it easier to extract various reagents required for gene sequencing from the scaffold module, thus ensuring the sequencing work of the gene sequencer.

[0045] In one optional embodiment, when each group of chip modules is connected to the valve group through multiple pipes, the valve group is connected to the manifold pump through multiple pipes; the manifold pump includes multiple syringes; wherein the number of pipes between the chip module and the valve group is the same as the number of pipes between the valve group and the manifold pump, and the number of pipes between the valve group and the manifold pump is the same as the number of syringes.

[0046] The syringe is used to control the current chip module to deliver the reagent reaction waste liquid to the valve assembly through a pipe corresponding to the syringe by pulling the piston on the syringe. The valve assembly is used to deliver the reagent reaction waste liquid to the syringe of the combined pump through a pipe corresponding to the syringe. The syringe is also used to push the reagent reaction waste liquid to the waste liquid module by pushing the piston on the syringe.

[0047] In implementation, each chip module is connected to a valve group via N pipes. The valve group is then connected to a manifold pump via N pipes. The manifold pump contains N syringes, each controlling one pipe. Each syringe may contain a piston, and the liquid in the fluid system is transported by pulling and pushing the piston. Here, N is a positive integer greater than 1.

[0048] If N is 4, then syringe 1 controls pipe 1, syringe 2 controls pipe 2, syringe 3 controls pipe 3, and syringe 4 controls pipe 4. For example, when syringe 1 pulls its piston (e.g., pulls the piston from the inside out), it controls the current chip module to input the reagent reaction waste liquid into the valve assembly through pipe 1. The valve assembly then delivers the reagent reaction waste liquid to the valve assembly through pipe 1. The valve assembly then delivers the reagent reaction waste liquid into syringe 1 through pipe 1. The operation of syringes 2, 3, and 4 is the same as that of syringe 1; multiple syringes can operate synchronously or asynchronously.

[0049] For example, see Figure 4 As shown, the example uses 4 groups of chip modules and 4 pipes (N). This valve group can include 12 solenoid valves. The connection relationships between the solenoid valves and the connection relationships between the chip modules and the solenoid valves are as follows: Figure 4 As shown in Table 2, the mapping relationship between the working status of each chip module and the valve position of the solenoid valve in this case is as follows.

[0050] Table 2. Mapping relationship between the operating status of each chip module and the valve position of the solenoid valve.

[0051] In practice, the chip module in operation is controlled by controlling the valve positions of each solenoid valve in the valve group. For example, when the valve positions of solenoid valves 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, and 512 are all G, the chip module 401 is in operation, meaning that a sample is being injected into the chip module 401.

[0052] For example, if the chip module 401 is in a working state, the four syringes 601 on the manifold pump 600 synchronously pull the piston, so that the chip module 401 receives the reagent and performs a reagent reaction based on the reagent to obtain reagent reaction waste liquid. The reagent reaction waste liquid is input into the valve group through four pipes. The solenoid valves 501, 502, 503, and 504 in the valve group input the reagent reaction waste liquid into the solenoid valves 509, 510, 511, and 512. Then, the solenoid valves 509, 510, 511, and 512 input the reagent reaction waste liquid into the syringes of the manifold pump through the four pipes.

[0053] Here, reagent reaction waste liquid can be transported through multiple pipelines, improving reagent transport efficiency and thus increasing the detection efficiency of the gene sequencer.

[0054] In one alternative implementation, see Figure 3 As shown, the fluid system also includes a cleaning module 800; the cleaning module 800 is connected to the combined pump 600 via a pipe 300.

[0055] The cleaning module 800 is used to deliver the stored cleaning reagent to the combined pump 600; the combined pump 600 is used to deliver the cleaning reagent to the syringe, and by pushing the piston of the syringe, deliver the cleaning reagent to the waste liquid module 700.

[0056] In implementation, a cleaning module can be set up, which can be a reagent kit containing cleaning reagents. The cleaning module delivers the stored cleaning reagents to the manifold pump 600. For example, by pulling the plunger of a syringe, the syringe can be controlled to draw the cleaning reagents from the cleaning module, and then by pushing the plunger of the syringe, the cleaning reagents can be delivered to the waste liquid module 700, thereby completing the cleaning of the pipeline between the cleaning module and the manifold pump, the pipeline between the waste liquid module and the manifold pump, and the syringe.

[0057] Here, by setting up a cleaning module, the tubing and syringe can be cleaned quickly using a syringe, so that the next reagent reaction can be carried out after cleaning. This reduces the impact of residual liquid in the tubing or syringe on the next reagent reaction, thereby improving the accuracy of gene sequencing.

[0058] In one optional embodiment, the valve assembly is further configured to determine the chip module to be cleaned among the multiple sets of chip modules based on the valve position of each of the solenoid valves; the manifold pump, after receiving the cleaning reagent, is further configured to: input the cleaning reagent to the chip module to be cleaned through the valve assembly.

[0059] In practice, one cleaning process can identify one chip module to be cleaned. The process of identifying the chip module to be cleaned is the same as that of identifying the current chip module. The process of identifying the chip module to be cleaned can be referred to the above description of identifying the current chip module, and will not be detailed here.

[0060] After receiving the cleaning reagent, the manifold pump can input the cleaning reagent to the chip module to be cleaned through the valve group, so as to clean the pipeline between the valve group and the manifold pump, the pipeline between the valve group and the chip module to be cleaned, and the chip module to be cleaned.

[0061] For example, the piston inside the syringe of the manifold pump can be controlled to move from the inside to the outside (i.e., pull the piston), so that the cleaning reagent stored in the cleaning module is delivered to the syringe through the pipeline. Then, by controlling the piston to move from the outside to the inside (i.e. push the piston), the cleaning reagent in the syringe is input from port B of the manifold pump to port A of the manifold pump, and then enters the pipeline through port A of the manifold pump, and then flows into the valve group through the pipeline, and then flows into the chip module to be cleaned through the valve group.

[0062] Here, cleaning reagents can also be used to clean the valve assembly and chip module, so that the cleaned chip module can be used for the next reagent reaction, thereby mitigating the impact of residual liquid in the chip module on the next reagent reaction and improving the accuracy of gene sequencing.

[0063] In one alternative embodiment, when the system includes a support module and a rotary valve, the chip module to be cleaned, upon receiving the cleaning reagent, is further configured to deliver the cleaning reagent to the reagent kit of the support module through the rotary valve.

[0064] In specific implementation, if the fluid system also includes a support module and a rotary valve, the chip module to be cleaned can also deliver the cleaning reagent to the rotary valve through a pipeline after receiving the cleaning reagent, and input it into the support module through the port of the rotary valve. Here, the rotary valve can be controlled to input the cleaning reagent into any reagent kit of the support module to complete the cleaning of the pipeline between the chip module and the rotary valve, and the pipeline between the rotary valve and the support module.

[0065] In one optional embodiment, the chip module includes at least an inlet, an outlet, and a temperature control module.

[0066] The chip module, when receiving reagents, performing a reagent reaction based on the reagents to generate reagent reaction waste liquid, and conveying the reagent reaction waste liquid to the combined pump through the valve group, is used for: receiving reagents from the inlet; controlling the reagent reaction temperature through the temperature control module; performing a reagent reaction based on the reagents at the reagent reaction temperature to generate reagent reaction waste liquid, and conveying the reagent reaction waste liquid from the outlet to the valve group, and conveying the reagent reaction waste liquid to the combined pump through the valve group.

[0067] In implementation, the chip module includes an inlet, an outlet, and a temperature control module. The temperature control module controls the temperature within the chip to ensure it meets the requirements of the reagent reaction. For example, reagents can enter the chip module through the inlet, react at the reagent reaction temperature controlled by the temperature control module, generate reagent reaction waste liquid, and flow out through the outlet, i.e., into the valve assembly. Cleaning reagents supplied by the valve assembly can flow into the chip module through the outlet and into the rotary valve through the inlet.

[0068] See Figure 5 As shown, combined with Figure 5The process of this fluid system is illustrated by way of example. The current chip module in operation among multiple chip modules is determined by the valve position of each solenoid valve in the valve group. Taking chip module 401 as an example, the pistons of the multiple syringes 601 of the manifold pump move from the inside out (i.e., pull the piston), causing the reagent needle on the support module 100 to draw reagent from the reagent kit through the reagent port and input the reagent into the pipeline 300, which then flows into the port of the rotary valve 200. The reagent then enters the pipeline through the common port V of the rotary valve, and flows into the chip module 401 through the inlet. Chip module 401 performs a reagent reaction at the reagent reaction temperature controlled by the temperature control module, producing a reagent reaction waste liquid. This waste liquid flows out through the outlet into the pipeline and then into the valve group 500. The connection relationship of each solenoid valve in the valve group 500 can be found in [reference needed]. Figure 4 As shown; Figure 5 Within the central valve assembly 500, the four solenoid valves located in the first row are respectively connected to... Figure 4 Solenoid valves 509, 510, 511, and 512 correspond to each other in the table. The eight solenoid valves in the second row correspond to each other. Figure 4 The solenoid valves 501 to 508 correspond to each other. Then, the liquid flows into the A port of the manifold pump through the valve group 500, and then into the syringe of the manifold pump through the B port. By controlling the piston of the syringe to move from the outside to the inside (i.e., pushing the piston), the reagent reaction waste liquid is transported to the waste liquid module 700 through the C port and the pipeline.

[0069] When cleaning a fluid system, the chip module to be cleaned can be identified from multiple chip modules by checking the valve positions of the various solenoid valves within the valve group. Taking chip module 401 as an example, the pistons of the multiple syringes in the manifold pump 600 move from the inside out (i.e., pull the pistons), causing the cleaning reagent stored in the cleaning module 800 to flow into the syringes through the pipes and the B port of the manifold pump. In one embodiment, by pushing the syringe, the cleaning reagent inside the syringe flows into the pipes through the C port of the manifold pump and is then transported to the waste liquid module 700, completing the cleaning of the pipes and syringes between the syringes and the waste liquid module. In another method, the cleaning reagent inside the syringe is pushed to the A port of the manifold pump 600 and flows into the pipeline. The cleaning reagent is then delivered to the valve group through the pipeline. Multiple solenoid valves in the valve group allow the cleaning reagent to enter the chip module 401 through the outlet, then flow into the pipeline through the inlet of the chip module 401, and finally into the rotary valve 200. The reagent then enters the corresponding port of the reagent kit through the common port V of the rotary valve, and finally flows into the reagent kit of the support module 100 through the corresponding port of the reagent kit, thus completing the cleaning of the chip module and the pipeline.

[0070] In the embodiments provided in this disclosure, it should be understood that the disclosed system can be implemented in other ways. The system embodiments described above are merely illustrative. For example, the division of units is only a logical functional division, and there may be other division methods in actual implementation. Furthermore, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Additionally, the coupling or direct coupling or communication connection shown or discussed may be indirect coupling or communication connection through some communication interfaces, devices, or units, and may be electrical, mechanical, or other forms.

[0071] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0072] In addition, the functional units in the various embodiments of this disclosure can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit.

[0073] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.

Claims

1. A fluid system for a gene sequencer, characterized in that, include: The system comprises a valve assembly, a manifold pump, a waste liquid module, pipelines, multiple chip modules, a support module, a rotary valve, and a cleaning module. Each chip module is connected to the valve assembly via pipelines, and the manifold pump is connected to both the valve assembly and the waste liquid module via pipelines. Each chip module contains multiple channels. The cleaning module is connected to the manifold pump via pipelines. The rotary valve is connected to the multiple chip modules. The valve group includes multiple solenoid valves, and the valve group is used to determine one chip module as the current chip module in the working state from the multiple sets of chip modules by using the valve position of each solenoid valve. The current chip module is used to receive reagents, perform reagent reactions based on the reagents, generate reagent reaction waste liquid, and transport the reagent reaction waste liquid to the combined pump through the valve group. The combined pump is used to transport the reagent reaction waste liquid to the waste liquid module; the combined pump includes multiple syringes; wherein, the number of pipes between the chip module and the valve group is the same as the number of pipes between the valve group and the combined pump, and the number of pipes between the valve group and the combined pump is the same as the number of syringes; The cleaning module is used to deliver the stored cleaning reagent to the combined pump; the combined pump is used to deliver the cleaning reagent to the syringe, and by pushing the piston of the syringe, deliver the cleaning reagent to the waste liquid module; The valve assembly is further configured to determine the chip module to be cleaned among the multiple sets of chip modules based on the valve positions of each of the solenoid valves; the manifold pump, after receiving the cleaning reagent, is further configured to input the cleaning reagent into the chip module to be cleaned through the valve assembly; the chip module to be cleaned, upon receiving the cleaning reagent, is further configured to deliver the cleaning reagent into the reagent kit of the support module through the rotary valve.

2. The fluid system according to claim 1, characterized in that, The support module is equipped with multiple reagent kits and a reagent needle corresponding to each reagent kit; each reagent needle is connected to one port of the rotary valve. The reagent needle is used to insert into the reagent port on the reagent kit to draw reagent from the reagent kit and deliver the drawn reagent to the current chip module through the port of the connected rotary valve.

3. The fluid system according to claim 1, characterized in that, The syringe is used to control the current chip module to deliver the reagent reaction waste liquid to the valve group through the pipe corresponding to the syringe by pulling the piston on the syringe; The valve assembly is used to deliver the reagent reaction waste liquid to the syringe of the combined pump through a pipe corresponding to the syringe; The syringe is also used to push the reagent reaction waste liquid to the waste liquid module by pushing the piston on the syringe.

4. The fluid system according to claim 1, characterized in that, The chip module includes at least a liquid inlet, a liquid outlet, and a temperature control module; The chip module, when receiving reagents, performing a reagent reaction based on the reagents to generate reagent reaction waste liquid, and conveying the reagent reaction waste liquid to the combined pump through the valve group, is used for: The reagent is received from the inlet; the reagent reaction temperature is controlled by the temperature control module; the reagent reaction is carried out based on the reagent at the reagent reaction temperature to generate reagent reaction waste liquid, and the reagent reaction waste liquid is transported from the outlet to the valve group, and the reagent reaction waste liquid is transported to the combined pump through the valve group.

5. The fluid system according to claim 1, characterized in that, The solenoid valve is a two-position three-way solenoid valve; each solenoid valve includes a first connection port, a second connection port, and a third connection port; the solenoid valve includes a first valve position indicating energization and a second valve position indicating de-energization. A portion of the multiple chip modules are connected to the first port of the solenoid valve, and another portion of the multiple chip modules are connected to the second port of the solenoid valve; the third port of the solenoid valve is connected to the combined pump.

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