Application of Cephalotaxus chinensis extract in cosmetics or preparation of medicines

Abstract

The present invention belongs to the technical field of cosmetics and medicines, and specifically relates to the use of Cephalotaxus truncatus extract in cosmetics or the preparation of medicines. The present invention provides the use of Cephalotaxus truncatus extract in cosmetics or the preparation of medicines, wherein the cosmetics include one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicines include one or more of anti-skin photoaging drugs, anti-skin oxidation drugs and anti-wrinkle drugs. The Cephalotaxus truncatus extract in the present invention is used in cosmetics or medicines with anti-skin photoaging, anti-skin oxidation and anti-wrinkle effects.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and medicines, and particularly relates to application of Cephalotaxus chinensis extract in cosmetics or preparation of medicines. Background Art

[0002] The skin is the main barrier of the human body, and its function is to protect the body's organs from external environmental stimuli (such as changes in temperature and humidity, ultraviolet rays, and pollutants). Due to a variety of internal and external factors, human skin gradually ages over time, and wrinkles are one of the signs of skin aging. Specifically, internal factors mainly include changes in genetic material, damage to cells by free radicals, metabolic disorders of the body, and other factors. External factors mainly include light, physical, chemical, mechanical and other factors; among them, light, especially chronic ultraviolet radiation, can increase skin free radicals and reactive oxygen species, reduce collagen, change the dermal structure, and cause keratin damage in the epidermis, causing photoaging, which is one of the main causes of wrinkle formation.

[0003] Meanwhile, reactive oxygen species are generated due to various physical, chemical and environmental factors (e.g., enzyme systems, reduction metabolism, chemicals, pollutants and photochemical reactions in the body). It is known that the reactive oxygen species generated cause cell aging and various diseases (including cancer) by destroying cell components (e.g., lipids, proteins, carbohydrates and DNA). In addition, various peroxides (including lipid peroxides) in the body generated by lipid peroxidation caused by these reactive oxygen species induce functional disorders and cause various diseases by oxidative damage to cells.

[0004] However, a large part of existing cosmetics or medicines can only play an anti-photoaging, anti-oxidation or anti-wrinkle role through physical covering, and the protective effect is limited, and they cannot effectively achieve the anti-photoaging, anti-oxidation and anti-wrinkle effect of the skin. Summary of the invention

[0005] The purpose of the present invention is to provide an application of a Cephalotaxus truncatus extract in cosmetics or the preparation of medicines. The Cephalotaxus truncatus extract provided by the present invention has excellent anti-skin photoaging, anti-wrinkle and anti-oxidation effects, and has significant effects when applied to cosmetics or medicines with anti-skin photoaging, anti-skin oxidation and anti-wrinkle effects.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides the use of Cephalotaxus chinensis extract in cosmetics or in the preparation of medicines. The cosmetics include one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicines include one or more of anti-skin photoaging drugs, anti-skin oxidation drugs and anti-wrinkle drugs.

[0008] Preferably, the preparation method of the Cephalotaxus chinensis extract comprises the following steps:

[0009] The Cephalotaxus truncatus extract is obtained by immersing the Cephalotaxus truncatus in an ethanol-water solvent and performing ultrasonic extraction; the mass percentage of ethanol in the ethanol-water solvent is 90-97%;

[0010] The solvent is removed from the Cephalotaxus affine extract to obtain the Cephalotaxus affine extract.

[0011] Preferably, the solvent removal obtains a Cephalotaxus chinensis crude extract; and the solvent removal further comprises the following steps:

[0012] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0013] The aqueous solution of the Cephalotaxus chinensis crude extract is heated and then an organic extractant is added for extraction to obtain an extracted organic phase; the organic extractant is ethyl acetate;

[0014] The solvent is removed from the extracted organic phase to obtain the pure Cephalotaxus chinensis extract.

[0015] Preferably, the solvent removal obtains a Cephalotaxus chinensis crude extract; and the solvent removal further comprises the following steps:

[0016] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0017] The aqueous solution of the Cephalotaxus chinensis crude extract is heated and then extracted with an organic extractant to obtain a raffinate aqueous phase; the organic extractant is ethyl acetate;

[0018] The solvent is removed from the raffinate water phase to obtain the Cephalotaxus chinensis extract.

[0019] Preferably, the solvent removal obtains a Cephalotaxus chinensis crude extract; and the solvent removal further comprises the following steps:

[0020] Dissolving the Cephalotaxus truncatus crude extract in a hot dilute acid solution for acidification to obtain a Cephalotaxus truncatus crude extract acid-water mixed solution, wherein the pH value of the mixed solution formed by dissolving the Cephalotaxus truncatus crude extract in the hot dilute acid solution is 2-3;

[0021] The acid-water mixture of the crude Cephalotaxus truncatus extract is separated into solid and liquid, and the solid phase product is taken to obtain the Cephalotaxus truncatus extract.

[0022] Preferably, the solvent removal obtains a crude Cephalotaxus chinensis extract; and after the solvent removal, the following steps are further included:

[0023] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0024] The aqueous solution of the Cephalotaxus truncatus crude extract is separated by column chromatography, eluted with acetone, the eluate is collected, and the solvent is removed from the eluate to obtain the Cephalotaxus truncatus extract.

[0025] Preferably, the ratio of the mass of Cephalotaxus chinensis to the volume of the ethanol-water solvent is 1 g: (3-5) mL; and the power of the ultrasonic extraction is 60-80W.

[0026] The present invention provides a cosmetic or medicine containing Cephalotaxus truncatus extract, wherein the cosmetic containing Cephalotaxus truncatus extract comprises Cephalotaxus truncatus extract and a cosmetic base, and the medicine containing Cephalotharynx truncatus extract comprises Cephalotharynx truncatus extract and a pharmaceutical excipient;

[0027] In the cosmetics or medicines containing the Cephalotaxus truncatus extract, the effective concentration of the Cephalotaxus truncatus extract is 10 μg / mL to 100 mg / mL.

[0028] Preferably, the dosage form of the cosmetic containing Cephalotaxus chinensis extract includes any one of cream, lotion, toner or essence.

[0029] Preferably, the dosage form of the medicine containing Cephalotaxus chinensis extract includes external solution, lotion, liniment, ointment, plaster, paste or patch.

[0030] The present invention provides the use of Cephalotaxus fortunei extract in cosmetics or medicines, wherein the cosmetics include one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicines include one or more of anti-skin photoaging drugs, anti-skin oxidation drugs and anti-wrinkle drugs. The Cephalotaxus fortunei extract of the present invention is used in cosmetics or medicines with anti-skin photoaging, anti-skin oxidation and anti-wrinkle effects. The present invention shows through the DPPH free radical scavenging rate test that the Cephalotaxus fortunei extract provided by the present invention has the effect of scavenging DPPH free radicals and has antioxidant activity. In the anti-UVB-induced cell damage experiment, the Cephalotaxus fortunei extract has excellent anti-UVB-induced cell damage effect. The present invention shows through animal model experiments that exposure to an intensity of 0.12 to 0.20 mw / cm 2 After 28 days under UVB, the back skin of the model group and the drug-treated group was photographed and the skin was taken for pathological tissue analysis. By comparison, it can be seen that the skin damage and aging of the mice in the model group were serious, while the damage and aging of the drug-treated group were significantly lighter. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The results of the test on the anti-UVB-induced cell damage of Cephalotaxus chinensis extract prepared in Examples 1 to 5;

[0032] Figure 2 This is a photo of the test of the Cephalotaxus truncatula extract prepared in Example 5 against UVB-induced photoaging of mouse skin;

[0033] Figure 3This is a tissue analysis diagram of the Cephalotaxus truncatula extract prepared in Example 5 in an anti-UVB-induced photoaging test on mouse skin. DETAILED DESCRIPTION

[0034] The present invention provides the use of Cephalotaxus chinensis extract in cosmetics or in the preparation of medicines. The cosmetics include one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicines include one or more of anti-skin photoaging drugs, anti-skin oxidation drugs and anti-wrinkle drugs.

[0035] In the present invention, unless otherwise specified, all preparation raw materials / components are commercially available products well known to those skilled in the art.

[0036] In the present invention, the Cephalotaxus fortunei Hooker extract is extracted from Cephalotaxus fortunei Hooker as a raw material. Among them, the Chinese herbal medicine Cephalotaxus fortunei Hooker is the whole herb of Cephalotaxus fortunei Hooker, a plant of the Cephalotaxaceae family. Cephalotaxus fortunei Hooker is distributed in Zhejiang, southern Anhui, Fujian, Jiangxi, Hunan, Hubei, southern Henan, southern Shaanxi, southern Gansu, Sichuan, Yunnan, Guizhou, Guangxi and Guangdong. The present invention provides the use of Cephalotaxus fortunei Hooker extract in anti-UVB-induced skin photoaging, anti-oxidation and anti-wrinkle functional cosmetics or medicines.

[0037] In the present invention, the method for preparing the Cephalotaxus chinensis extract preferably comprises the following steps:

[0038] The Cephalotaxus truncatus extract is obtained by immersing the Cephalotaxus truncatus in an ethanol-water solvent and performing ultrasonic extraction; the mass percentage of ethanol in the ethanol-water solvent is 90-97%;

[0039] The solvent is removed from the Cephalotaxus affine extract to obtain the Cephalotaxus affine extract.

[0040] The present invention soaks Cephalotaxus truncatus in an ethanol-water solvent and ultrasonically extracts to obtain a Cephalotaxus truncatus extract; the mass percentage of ethanol in the ethanol-water solvent is 90-97%. In the present invention, before the soaking, the present invention preferably pre-treats the Cephalotaxus truncatus, and in the present invention, the pre-treatment preferably includes: drying and crushing the Cephalotaxus truncatus in sequence, and the present invention has no special requirements for the specific implementation process of the drying and crushing.

[0041] In the present invention, the ratio of the mass of Cephalotaxus truncatus to the volume of the ethanol-water solvent is preferably 1 g: (3-5) mL; the power of the ultrasonic extraction is preferably 60-80 W. The number of ultrasonic extractions is preferably 3 times, and the present invention preferably combines the extracts obtained from each ultrasonic extraction to obtain the Cephalotaxus truncatus extract.

[0042] After obtaining the Cephalotaxus truncatus extract, the present invention removes the solvent from the Cephalotaxus truncatus extract to obtain the Cephalotaxus truncatus extract. In the present invention, the solvent removal is preferably carried out by water bath rotary evaporation, and the temperature of the solvent removal is preferably 57°C.

[0043] In the present invention, the solvent removal is performed to obtain a crude Cephalotaxus chinensis extract; and after the solvent removal, the following steps are preferably further included:

[0044] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0045] The aqueous solution of the Cephalotaxus chinensis crude extract is heated and then an organic extractant is added for extraction to obtain an extracted organic phase; the organic extractant is ethyl acetate;

[0046] The solvent is removed from the extracted organic phase to obtain the pure Cephalotaxus chinensis extract.

[0047] The present invention dissolves the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution. In the present invention, the water is preferably pure water. The ratio of the mass of the Cephalotaxus chinensis crude extract to the volume of the water is preferably 5g:100mL.

[0048] After obtaining the aqueous solution of the Cephalotaxus truncatus crude extract, the present invention heats the aqueous solution of the Cephalotaxus truncatus crude extract and then adds an organic extractant for extraction to obtain an extracted organic phase; the organic extractant is ethyl acetate. In the present invention, the temperature of the aqueous solution of the Cephalotaxus truncatus crude extract after heating is preferably 57° C. The ethyl acetate is preferably industrial ethyl acetate. In the present invention, the volume ratio of the water to the organic extractant is preferably 10:8. In the present invention, the number of extractions is preferably 3 times, and the present invention preferably combines the organic phases extracted each time to obtain the extracted organic phase.

[0049] After obtaining the extracted organic phase, the present invention removes the solvent from the extracted organic phase to obtain the Cephalotaxus chinensis extract. In the present invention, the solvent removal method is preferably evaporation to dryness.

[0050] In the present invention, the solvent removal is performed to obtain a crude Cephalotaxus chinensis extract; and after the solvent removal, the following steps are preferably further included:

[0051] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0052] The aqueous solution of the Cephalotaxus chinensis crude extract is heated and then extracted with an organic extractant to obtain a raffinate aqueous phase; the organic extractant is ethyl acetate;

[0053] The solvent is removed from the raffinate water phase to obtain the Cephalotaxus chinensis extract.

[0054] The present invention dissolves the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution. In the present invention, the water is preferably pure water. The ratio of the mass of the Cephalotaxus chinensis crude extract to the volume of the water is preferably 5g:100mL.

[0055] After obtaining the aqueous solution of the Cephalotaxus truncatus crude extract, the present invention heats the aqueous solution of the Cephalotaxus truncatus crude extract and adds an organic extractant for extraction to obtain an extracted organic phase; the organic extractant is ethyl acetate. In the present invention, the temperature of the aqueous solution of the Cephalotaxus truncatus crude extract after heating is preferably 57° C. The ethyl acetate is preferably industrial ethyl acetate. In the present invention, the volume ratio of the water to the organic extractant is preferably 10:8. In the present invention, the number of extractions is preferably 3 times, and the present invention preferably combines the raffinate phases of each extraction to obtain the raffinate aqueous phase.

[0056] After obtaining the raffinate aqueous phase, the present invention removes solvent from the raffinate aqueous phase to obtain the Cephalotaxus chinensis extract. In the present invention, the solvent removal method is preferably evaporation to dryness.

[0057] In the present invention, the solvent removal is performed to obtain a crude Cephalotaxus chinensis extract; and after the solvent removal, the following steps are preferably further included:

[0058] Dissolving the Cephalotaxus truncatus crude extract in a hot dilute acid solution for acidification to obtain a Cephalotaxus truncatus crude extract acid-water mixed solution, wherein the pH value of the mixed solution formed by dissolving the Cephalotaxus truncatus crude extract in the hot dilute acid solution is 2-3;

[0059] The acid-water mixture of the crude Cephalotaxus truncatus extract is separated into solid and liquid, and the solid phase product is taken to obtain the Cephalotaxus truncatus extract.

[0060] The present invention dissolves the Cephalotaxus truncatus crude extract in a hot dilute acid solution for acidification to obtain a Cephalotaxus truncatus crude extract acid-water mixed solution, and the pH value of the mixed solution formed by the Cephalotaxus truncatus crude extract dissolved in the hot dilute acid solution is 2-3. In the present invention, the temperature of the hot dilute acid solution is preferably 60°C, and the dilute acid solution is preferably a dilute hydrochloric acid solution. The dilute hydrochloric acid solution is formed by mixing a concentrated hydrochloric acid solution and pure water, the mass percentage of the concentrated hydrochloric acid is preferably 37%, and the volume ratio of the concentrated hydrochloric acid to the pure water is preferably 1: (33-60). In the present invention, the pH value of the mixed solution formed by the Cephalotaxus truncatus crude extract dissolved in the hot dilute acid solution is more preferably 2.5. In the present invention, the dissolution is preferably carried out under stirring, and the stirring time is preferably ≥2h; the present invention preferably ensures that the alkaloid components in the total extract are fully acidified to form organic salts free in water by stirring. In the present invention, the acidification temperature is preferably 2-4°C, the acidification time is preferably 12h, and the acidification is preferably carried out under static conditions.

[0061] After obtaining the acid-water mixture of the crude extract of Cephalotaxus truncatus, the present invention separates the solid-liquid mixture of the crude extract of Cephalotaxus truncatus into solid-liquid, takes the solid phase product, and obtains the Cephalotaxus truncatus extract. In the present invention, the solid-liquid separation is preferably carried out in a vacuum filter, and the obtained solid phase product is preferably washed to obtain the Cephalotaxus truncatus extract. In the present invention, the washing preferably includes the following steps: adding the solid phase product to dilute hydrochloric acid, the pH value of the obtained suspension is preferably 2.5-3, the dilute hydrochloric acid is preferably mixed with concentrated hydrochloric acid and pure water according to a volume ratio of preferably 1:80, and the mass percentage of the concentrated hydrochloric acid is preferably 37%; heating the suspension to 70°C and stirring for 30-60min, hot filtering, and obtaining the washed solid phase product. In the present invention, the number of washings is preferably 4. The solid phase product after the last washing is the Cephalotaxus truncatus extract.

[0062] In the present invention, the solvent is removed to obtain a crude extract of Cephalotaxus chinensis; the method for preparing the Cephalotaxus chinensis extract preferably further comprises the following steps:

[0063] dissolving the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution;

[0064] The aqueous solution of the Cephalotaxus truncatus crude extract is separated by column chromatography, eluted with acetone, the eluate is collected, and the solvent is removed from the eluate to obtain a pure product of the Cephalotaxus truncatus extract.

[0065] The present invention dissolves the Cephalotaxus chinensis crude extract in water to obtain a Cephalotaxus chinensis crude extract aqueous solution. In the present invention, the water is preferably pure water. The present invention has no special requirements on the amount of the water, as long as the Cephalotaxus chinensis crude extract is completely dissolved.

[0066] After obtaining the aqueous solution of the crude extract of Cephalotaxus truncatus, the present invention performs column chromatography separation on the aqueous solution of the crude extract of Cephalotaxus truncatus, uses acetone for elution, collects the eluate, removes the solvent from the eluate, and obtains the Cephalotaxus truncatus extract. In the present invention, the column chromatography separation is preferably macroporous resin column chromatography separation. The column length of the chromatographic column of the column chromatography separation is preferably 40 cm, and the diameter is preferably 2.8 cm. In the present invention, the time for the sample adsorption of the aqueous solution of the crude extract of Cephalotaxus truncatus is ≥2h. After the sample is loaded, before the present invention uses the acetone for elution, the present invention preferably uses distilled water, ethanol-water with a mass content of 20%, ethanol-water with a mass content of 40%, ethanol-water with a mass content of 60%, ethanol-water with a mass content of 80%, and ethanol-water with a mass content of 95% to elute and remove impurities in sequence, and then uses acetone for elution and collects the eluate. The present invention preferably uses a rotary concentration and evaporation method to remove the solvent.

[0067] The invention provides a cosmetic or medicine containing Cephalotaxus truncatus extract. The cosmetic containing Cephalotaxus truncatus extract comprises Cephalotaxus truncatus extract and a cosmetic matrix, and the medicine containing Cephalotaxus truncatus extract comprises Cephalotaxus truncatus extract and a pharmaceutical excipient.

[0068] In the present invention, the cosmetic base is preferably a cosmetic base and additives permitted for use in the cosmetics supervision and management regulations.

[0069] In the present invention, in the cosmetics containing Cephalotaxus truncatus extract, the effective concentration of the Cephalotaxus truncatus extract is preferably 10 μg / mL to 100 mg / mL, and more preferably 15 μg / mL to 50 mg / mL.

[0070] In the present invention, in the medicine containing Cephalotaxus truncatus extract, the effective concentration of the Cephalotaxus truncatus extract is preferably 10 μg / mL to 100 mg / mL, more preferably 15 μg / mL to 50 mg / mL, and most preferably 2 mg / mL.

[0071] In the present invention, the dosage form of the cosmetic containing Cephalotaxus chinensis extract preferably includes any one of cream, lotion, toner or essence.

[0072] In the present invention, the dosage form of the medicine containing Cephalotaxus chinensis extract preferably includes external solution, lotion, liniment, ointment, plaster, paste or patch, more preferably liniment.

[0073] In the present invention, when the dosage form of the medicine containing Cephalotaxus truncatula extract is preferably an ointment, the excipient is a solvent, and the solvent includes isopropanol and anhydrous ethanol. The volume ratio of isopropanol to anhydrous ethanol is preferably 7:3.

[0074] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0075] Example 1

[0076] Weigh 100 g of dried Cephalotaxus fortunei, grind it into powder, soak Cephalotaxus fortunei in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at a power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57°C to obtain a crude Cephalotaxus fortunei extract (extraction rate is 13.87%), recorded as Cephalotaxus fortunei extract 1.

[0077] Example 2

[0078] Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at a power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a crude Cephalotaxus truncatus extract;

[0079] Weigh 5 g of crude extract of Cephalotaxus ternata and add 100 mL of pure water to dissolve it. After heating to 57°C, add 80 mL of industrial ethyl acetate for extraction. Repeat three times, combine the ethyl acetate layers, evaporate the ethyl acetate in the ethyl acetate layers to obtain the Cephalotaxus ternata extract with a yield of 33.35%, recorded as Cephalotaxus ternata extract II.

[0080] Example 3

[0081] Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at an ultrasonic power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a crude Cephalotaxus truncatus extract;

[0082] Weigh 5 g of crude extract of Cephalotaxus ternata and add 100 mL of pure water to dissolve it. After heating to 57°C, add 80 mL of industrial ethyl acetate to extract, repeat three times, combine the water layers, evaporate the water in the water layers to obtain the Cephalotaxus ternata extract with a yield of 61.91%, recorded as Cephalotaxus ternata extract three.

[0083] Example 4

[0084] Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at an ultrasonic power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a crude Cephalotaxus truncatus extract (extraction rate is 13.87%);

[0085] Weigh 3.89 g of the Cephalotaxus truncatus crude extract and add hot dilute hydrochloric acid at a temperature of 60° C. while it is hot. The dilute hydrochloric acid is obtained by diluting concentrated hydrochloric acid (37%) with pure water, wherein the volume ratio of concentrated hydrochloric acid to pure water is 1:33. Stir well, adjust the pH value of the mixed solution formed by mixing the Cephalotaxus truncatus crude extract and the hot dilute hydrochloric acid to 2.5, stir for more than half an hour to ensure that the alkaloid components in the Cephalotaxus truncatus crude extract are fully acidified to form organic salts free in water. The mixed acid solution (pH value to 2.5) after acid adjustment needs to be left to stand at 2° C. for 12 hours to obtain an acid-water mixture;

[0086] The acid-water mixture that had been allowed to stand for 12 hours was transferred into a vacuum filter, and the clarified acid solution was discarded. Dilute hydrochloric acid (the volume ratio of 37% concentrated hydrochloric acid to pure water was 1:80) was added to the residue obtained, and the pH value of the mixed solution was controlled to be 2.5. The mixture was heated to 70°C, stirred for 30 minutes, and filtered hot to obtain the first residue washing filtrate and discard it. The first residue washing was repeatedly acid-washed 4 times according to the first washing, and the filtrate obtained was Cephalotaxus quinquefolius extract with a yield of 18.92%, recorded as Cephalotaxus quinquefolius extract IV.

[0087] Example 5

[0088] Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at an ultrasonic power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a crude Cephalotaxus truncatus extract (extraction rate is 13.87%);

[0089] Take 1g of the crude extract of Cephalotaxus truncatus and dissolve it in pure water, apply it to a macroporous resin separation column with a length of 40cm and a diameter of 2.8cm, adsorb for 3 hours, and elute with distilled water, 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, 95% ethanol, and acetone in sequence. Collect the eluate obtained by eluting with acetone, concentrate and evaporate the acetone eluate to dryness, and obtain the Cephalotaxus truncatus extract (yield is 5.27%), which is recorded as Cephalotaxus truncatus extract V.

[0090] Test Example 1

[0091] DPPH free radical scavenging rate test of five Cephalotaxus chinensis extracts prepared in Examples 1 to 5

[0092] DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical is dark purple due to its unpaired electrons, and its scavenging ability can be measured by the absorbance at 517nm. Add 50μL of the sample to be tested to a 96-well plate, and then add 50μL of DPPH methanol solution (0.208mM). After standing at room temperature in the dark for 40 minutes, measure the absorbance at 517nm. The test results are shown in Table 1. From the results in Table 1, it can be seen that the five Cephalotaxus extracts prepared in Examples 1 to 5 all have the effect of scavenging free radicals and have antioxidant activity.

[0093] Table 1 DPPH radical scavenging rates of five Cephalotaxus chinensis extracts prepared in Examples 1 to 5

[0094]

[0095] Test Example 2

[0096] Test of the five Cephalotaxus truncatula extracts prepared in Test Examples 1 to 5 against UVB-induced cell damage

[0097] The Cephalotaxus chinensis extract prepared in each example was subjected to three parallel experiments, and each experiment set three sample concentrations, namely 400, 200, and 100 μg / mL, with three replicate wells for each concentration. 96-well plate, 1×10 4 After inoculating cells overnight, samples were added the next day and irradiated with UVB for 27 minutes one hour after adding samples. The irradiation dose was 300mJ / cm 2 The cell viability was determined 24 hours later. The cell viability was determined using the MTT method. Figure 1 As shown in the figure, it can be seen that the five Cephalotaxus extracts prepared in Examples 1 to 5 can resist the cell damage caused by UVB and have anti-photoaging activity.

[0098] Application Example 1

[0099] The Cephalotaxus truncatus extract prepared in Example 5 was dissolved in a matrix with a volume ratio of isopropanol:anhydrous ethanol=7:3 to prepare a clear and uniform solution of 2 mg / mL to obtain a Cephalotaxus truncatus extract sample.

[0100] Test Example 3

[0101] Test on the anti-UVB-induced photoaging of mouse skin by using Cephalotaxus chinensis extract sample prepared in Application Example 1;

[0102] Female Kunming mice that had been adaptively raised for 3 days were selected, weighing 25-30g, and the hair on their backs was removed. They were divided into blank control group, model group, and sample group with 6 mice in each group. One hour before ultraviolet irradiation, the sample group mice were given Cephalotaxus truncatus extract on their backs, with 100 μL of the sample applied to each mouse, and the applied skin area was about 2 cm 2 100 μL of blank matrix was applied to the blank control and model groups. Then the mice were exposed to an intensity of 0.12-0.20 mw / cm 2 Irradiate the mice with UVB for 30-40 minutes; 1 hour after UV irradiation, apply the samples again according to the above route and dosage, irradiate once a day, and apply the samples twice for 28 days. After the expiration, anesthetize the mice, take pictures of the skin on the back of the mice, and then remove them for HE staining and pathological tissue analysis. The results are as follows Figure 2 and Figure 3 As shown in the figure, it can be seen from the comparison that the skin damage and aging of mice in the model group are serious, while the damage and aging of mice in the drug-treated group are significantly lighter.

[0103] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. Other embodiments can be obtained based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Use of Cephalotaxus chinensis extract in the preparation of cosmetics or medicines, wherein the cosmetics are one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicine is an anti-wrinkle drug; The preparation method of the Cephalotaxus chinensis extract comprises the following steps: Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at an ultrasonic power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a Cephalotaxus truncatus extract, wherein the extraction rate of the Cephalotaxus truncatus extract is 13.87%; the Cephalotaxus truncatus is a plant of the Cephalotaxaceae family, and the Latin name of the Cephalotaxus truncatus plant of the Cephalotaxaceae family is Cephalotaxus fortunei Hooker .

2. A cosmetic or medicine containing Cephalotaxus chinensis extract, characterized in that: The cosmetic containing Cephalotaxus truncatus extract comprises Cephalotaxus truncatus extract and a cosmetic base, and the medicine containing Cephalotharynx truncatus extract comprises Cephalotharynx truncatus extract and a pharmaceutical excipient; the cosmetic is one or more of anti-skin photoaging cosmetics, anti-skin oxidation cosmetics and anti-wrinkle cosmetics; the medicine is an anti-wrinkle medicine; The preparation method of the Cephalotaxus chinensis extract comprises the following steps: Weigh 100 g of dried Cephalotaxus truncatus, grind it into powder, soak Cephalotaxus truncatus in 300 mL of ethanol-water with an ethanol content of 90%, perform ultrasonic extraction at an ultrasonic power of 60 W, repeat the extraction three times, combine the three extracts, and evaporate to dryness in a constant temperature water bath at 57° C. to obtain a Cephalotaxus truncatus extract, wherein the extraction rate of the Cephalotaxus truncatus extract is 13.87%; the Cephalotaxus truncatus is a plant of the Cephalotaxaceae family, and the Latin name of the Cephalotaxus truncatus plant of the Cephalotaxaceae family is Cephalotaxus fortunei Hooker ; In the cosmetics or medicines containing the Cephalotaxus truncatus extract, the effective concentration of the Cephalotaxus truncatus extract is 10 μg / mL to 100 mg / mL.

3. The cosmetic or medicine containing Cephalotaxus chinensis extract according to claim 2, characterized in that: The dosage forms of the cosmetics containing Cephalotaxus truncatula extract include cream, emulsion, lotion or essence.

4. The cosmetic or medicine containing Cephalotaxus chinensis extract according to claim 2, characterized in that: The dosage form of the medicine containing Cephalotaxus truncatula extract includes external solution, ointment, plaster, paste or patch.

5. The cosmetic or medicine containing Cephalotaxus chinensis extract according to claim 2, characterized in that: The dosage form of the medicine containing Cephalotaxus chinensis extract includes lotion or ointment.

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