An African swine fever virus antibody detection method and its application
By screening and connecting the dominant epitope regions of the African swine fever virus p30, p54, and p72 proteins, a highly sensitive and specific ELISA detection method was established, which solved the false negative and false positive problems of the existing detection methods, and achieved more accurate virus antibody detection.
Patent Information
- Application Number
- CN202211118203.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-09-15
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2042-09-15
AI Technical Summary
The existing African swine fever virus antibody detection methods are prone to false negative and false positive, and are costly, which cannot effectively meet the needs of vaccine effectiveness evaluation.
The dominant epitope regions of the African swine fever virus p30, p54, and p72 proteins were screened, and these epitope regions were connected into a recombinant protein through molecular biological technology to serve as coated antigens detected by ELISA to establish a highly sensitive and specific detection method.
It improves the sensitivity and specificity of the detection, reduces the false negative and false positive rates, provides more effective ASF virus prevention and control detection methods, and provides a material basis for the evaluation of vaccine effectiveness.