An organic polypeptide enzyme activity promoter and its preparation method

Abstract

The present application relates to the field of fertilizers, and specifically discloses an organic polypeptide enzyme activity promoter and a preparation method thereof; an organic polypeptide enzyme activity promoter, comprising the following raw materials in parts by weight: animal brain, animal pancreas, soybean, soybean shell, peanut, loaded porous filler, and adhesion promoter; the loaded porous filler is prepared by loading calcium carbonate micropowder and tributyrin in sequence after hydrophobic modification of a porous filler; the preparation method thereof is as follows: weighing the animal pancreas, chopping it, mixing it with part of the adhesion promoter, then adding the loaded porous filler and mixing it evenly, and treating it to obtain a primary mixture; weighing the animal brain, soybean, and peanut, chopping them, mixing them evenly, and obtaining a mixture; mixing the primary mixture evenly with soybean shell, then adding the mixture and the remaining adhesion promoter, mixing evenly, and granulating to obtain a finished product; after the application of the organic polypeptide enzyme activity promoter, the crop is not easily affected by drought in the early growth stage, and is not easily affected by floods in the later growth stage, thereby obtaining a higher yield.

Description

Technical Field

[0001] The present application relates to the field of fertilizers, and more specifically, to an organic polypeptide enzyme activity promoter and a preparation method thereof. Background Art

[0002] Long-term use of chemical fertilizers can easily cause soil acidification, which in turn activates the activity of heavy metals in the soil. Heavy metal pollutants are not only unable to be degraded by microorganisms, but may also continue to accumulate in organisms through the food chain, and can even be converted into more toxic methyl compounds, eventually accumulating in the human body and endangering health. Therefore, once the soil environment is contaminated by heavy metals, it is difficult to completely eliminate it.

[0003] Based on this, organic fertilizer has gradually become popular among people. Organic fertilizer mainly comes from plants and animals, and is processed from biological materials, animal and plant waste, and plant residues. It eliminates harmful substances and is rich in a large number of beneficial substances, including a variety of organic acids, peptides, and nutrients including nitrogen, phosphorus, and potassium. It can not only provide comprehensive nutrients for the growth of crops, but also has a long fertilizer effect, can increase and renew organic matter in the soil, thereby promoting high-quality and high-quantity growth of crops.

[0004] In the prior art, a Chinese invention patent application with publication number CN104961544A discloses an organic polypeptide enzyme activity promoter, which is prepared by the following preparation method: taking 10 to 20 parts by weight of pig or cattle brain, 10 to 20 parts by weight of pig or cattle pancreas, 10 to 20 parts by weight of soybeans, 10 to 20 parts by weight of walnuts, and 10 to 20 parts by weight of peanuts; using a chopper to chop the materials in step (1); and subjecting the materials to harmless treatment, filtering, and concentration to obtain a liquid organic polypeptide enzyme activity promoter; the liquid organic polypeptide enzyme activity promoter can promote the absorption of organic fertilizer by plants, improve the degree of soil granulation, thereby improving the ability to retain water and fertilizer, and making the stomatal wall of crops under drought conditions achieve drought resistance.

[0005] However, in the Northeast region, in recent years, drought problems often occur in the early growth stage after crop sowing, and flood disasters occur near the autumn harvest, resulting in a relatively serious impact on crop growth; the single water and fertilizer retention effect in the existing technology can make crops drought-resistant in the early stage, but when flood disasters come, soil water retention makes the soil structure dense and the oxygen content decreases, making the crop roots in the soil prone to root rot due to lack of oxygen, thereby affecting the growth of crops.

[0006] Therefore, there is an urgent need to prepare a new regulating promoter to be placed in the soil, which has a good drought resistance effect in the early growth stage of crop sowing, and makes the crops have a good flood resistance effect when the autumn harvest is approaching 3-4 months later, thereby ensuring that the crops can grow well throughout their life cycle to increase crop yields. Summary of the invention

[0007] In order to prepare a new regulating promoter and place it in the soil, which has a good drought resistance effect in the early growth stage of crop sowing, and makes the crops have a good flood resistance effect when the autumn harvest is approaching 3-4 months later, thereby ensuring that the crops can grow well throughout their life cycle to increase crop yields, the application provides an organic polypeptide enzyme activity promoter and a preparation method thereof.

[0008] In the first aspect, the present application provides an organic polypeptide enzyme activity promoter, which adopts the following technical scheme:

[0009] An organic polypeptide enzyme activity promoter comprises the following raw materials in parts by weight: 10-15 parts of animal brain, 15-30 parts of animal pancreas, 10-18 parts of soybean, 5-12 parts of soybean shell, 10-18 parts of peanut, 10-20 parts of loaded porous filler and 5-15 parts of adhesion promoter; the loaded porous filler is prepared by sequentially loading calcium carbonate micropowder and tributyrin on a multi-porous filler after hydrophobic modification.

[0010] By adopting the above technical scheme, animal brain, animal pancreas, loaded porous filler, soybean, soybean shell and peanut are matched; in the early stage of crop growth, the polypeptides in animal brain and animal pancreas and the nutrients in soybean and peanut are used to promote crop growth; and the loaded porous filler and animal pancreas are matched to retain water and resist drought in the early stage of crop growth; at the same time, in the late stage of crop growth, the hydrophobicity of the multi-porous filler is combined with the hydrophobicity of the soybean shell to further promote the excess water in the soil to flow out through the drainage channel, and try to avoid the long-term accumulation of water in the soil, and the air permeability of the multi-porous filler is used to make the crop have a better anti-waterlogging effect in the late stage of growth; thereby ensuring that the crop can grow well throughout its life cycle to increase the crop yield.

[0011] After the promoter is applied to the soil surface, the animal pancreas and the loaded porous filler cooperate with each other, and the trypsin in the animal pancreas reacts with the tributyrin on the surface of the loaded porous filler to generate butyric acid and glycerol. The butyric acid can not only adjust the soil pH to promote the growth and reproduction of beneficial bacteria in the soil, but also react with the calcium carbonate in the loaded porous filler to generate calcium butyrate, water and carbon dioxide; the calcium ions in the calcium butyrate are convenient for forming a gel structure in the soil, and the water absorption and retention effect of glycerol makes the soil have a better water retention effect; the air permeability of the pores of the porous filler, the ventilation effect of the generated carbon dioxide and the loosening effect of soybean shells in the soil make the soil have a better air permeability, which is conducive to the rapid growth of crops after sowing; and the nutrients in soybeans, soybean shells, peanuts and animal brains are combined to further promote the growth of crops, so that the crops have a better drought resistance effect in the early growth period after sowing, ensuring the rapid and healthy growth of crops.

[0012] As time goes by, crops continue to grow, and the open-porous filler utilizes the sustained-release effect of its pore structure to slowly produce and release calcium butyrate, while the calcium ions also slowly form a gel to retain water. At the same time, calcium butyrate can inhibit the growth and reproduction of harmful bacteria in the soil for a long time, and instead promote the growth and reproduction of beneficial bacteria, thereby promoting the growth of crops; combined with the slow release of nutrients in soybeans and peanuts, it can promote crop growth for a long time.

[0013] When floods occur in the middle and late stages of crop growth, calcium ions in the soil are easily leached. At this time, the hydrophobic effect of the porous structure in the open-porous filler is combined with the water dispersion and drainage effect of the residual soybean shells, making it difficult for water to accumulate in the soil and flow out along the drainage channel. It also has good ventilation effect, reducing the impact of floods on the late stage of crop growth.

[0014] Preferably, the loaded porous filler is prepared by the following method:

[0015] Weighing a porous filler, placing it in a phosphatidylserine solution, dispersing and stirring it, then soaking it for 2-5 minutes, adding calcium carbonate powder, dispersing it, then taking out the porous filler, and drying it to obtain a hydrophobically modified filler;

[0016] The hydrophobically modified filler is placed in a tributyrin solution for dispersion and soaking, then taken out and dried to obtain a finished loaded porous filler.

[0017] By adopting the above technical scheme, the multi-opening filler, phosphatidylserine, calcium carbonate powder and tributyrin are matched. First, the adsorption effect of the multi-opening filler on phosphatidylserine is utilized to make the hydrophilic group of phosphatidylserine face the inside of the multi-opening filler and the hydrophobic group face outward. When the pores of the multi-opening filler are loaded with calcium carbonate powder, the hydrophobic group in the tributyrin liquid is attracted to the hydrophobic group of phosphatidylserine, so that the hydrophobic group of tributyrin faces the inside of the multi-opening filler and the hydrophilic group faces outward. The outer surface of the prepared loaded porous filler is the hydrophilic group of tributyrin. The loaded porous filler can play a hydrophilic and water-retaining role in the soil during the dry period, and then play a hydrophobic role during the flood period, so as to avoid the long-term condensation of water near the root system of crops, which will affect the water and oxygen absorption of the root system of crops, thereby ensuring the growth of crops.

[0018] Preferably, the open porous filler is composed of zeolite and perlite in a weight ratio of 1:1-3.

[0019] By adopting the above technical scheme, zeolite and perlite are combined, and the more open pore structures of zeolite and perlite are combined with the hydrophilicity of the pore structure, so that the multi-open porous filler can attract the hydrophilic groups in the phosphatidylserine solution and connect with the inner side of the multi-open porous filler, so that the hydrophobic groups face outward; then calcium carbonate micropowder and tributyrin are loaded to ensure that the hydrophobic groups in the tributyrin are attracted and connected with the hydrophobic groups in the phosphatidylserine solution, so that the hydrophilic groups in the tributyrin face outward, and the multi-open porous filler has hydrophilic and water-retaining properties in the early stage of crop growth; and the multi-open porous filler has hydrophobicity in the middle and late stages of growth, so as to ensure that the crops can grow well throughout their life cycle.

[0020] Preferably, the calcium carbonate powder is composed of calcium carbonate particles and hot-melt propolis liquid in a mass ratio of 1:0.2-0.4.

[0021] By adopting the above technical scheme, calcium carbonate particles and hot-melt propolis liquid are matched, and the hot-melt propolis liquid is used to coat the calcium carbonate particles to form a propolis film on the surface of the calcium carbonate particles. The lipophilicity of the propolis film is combined with the attraction and connection effect of the lipophilic groups on the outside of the phosphatidylserine in the pores of the multi-open porous filler, so that the calcium carbonate powder can enter the internal pores of the multi-open porous filler and be loaded in the pore structure more stably; with the entry of tributyrin, the lipophilicity of propolis and the lipophilicity of phosphatidylserine are combined with the lipophilic groups of tributyrin to further improve the attachment of tributyrin to the inside of the pores of the multi-open porous filler, so that the hydrophilic groups of tributyrin are facing outward; the hydrophilic and water-retaining effects are achieved in the early stage of crop growth, and the hydrophobic groups in the pores of the multi-open porous filler are used in the middle and late stages of crop growth to further promote water loss, and try to avoid water agglomeration in the soil, which affects the growth of crops in the middle and late stages of growth.

[0022] Preferably, the soybean hull is obtained by treating the surface of crushed soybean hull with a sodium alginate solution.

[0023] By adopting the above technical scheme, soybean shells and loaded porous fillers are matched, and the carboxyl groups in the sodium alginate on the surface of the soybean shells are combined with the calcium ions generated in the loaded porous fillers and the moisture in the soil, so as to further promote the formation of calcium ion gel; and with the support structure of the soybean shells, the soil structure is relatively loose, which is convenient for the circulation and replacement of water and oxygen, thereby promoting hydrophilicity and moisture retention in the early stage of crop growth, so as to promote the rapid growth of crop roots, thereby further promoting the rapid growth of crops; as the crops grow, the calcium ions are gradually utilized, and the gel disintegrates. Even if floods occur in the later stage of crop growth, it is not easy for the soil to retain water. Instead, the hydrophobicity of the soybean shells and the loaded porous fillers is utilized to promote water loss, and the accumulation of water in the soil is avoided as much as possible, so as to avoid the impact of floods in the later stage of crop growth.

[0024] Preferably, the animal pancreas is one or more of porcine pancreas and bovine pancreas.

[0025] By adopting the above technical solution and utilizing the higher content of trypsin and other proteases in pig pancreas and bovine pancreas, the promoter can play a role in the soil for a long time and efficiently.

[0026] Preferably, the adhesion promoter is an aqueous solution of sodium hydroxymethyl cellulose.

[0027] By adopting the above technical scheme, the sodium hydroxymethyl cellulose aqueous solution and the loaded porous filler are matched, and the hydroxyl groups in the sodium hydroxymethyl cellulose aqueous solution are used to match the carboxyl groups in the tributyrin on the surface of the loaded porous filler, so as to further promote the adhesion between the adhesion promoter and the loaded porous filler, thereby ensuring the hydrophilic and moisturizing effect of the promoter in the early stage of crop growth, thereby ensuring the growth of crops.

[0028] Preferably, the animal brain is one or more of pig brain, cow brain and rabbit brain.

[0029] By adopting the above technical solution, the high content of protein in animal brain, combined with the high content of protein in soybeans and peanuts, further provides rich nutrients to the soil, thereby promoting the rapid absorption of nutrients by crop roots to promote crop growth.

[0030] In a second aspect, the present application provides a method for preparing an organic polypeptide enzyme activity promoter, using the following technical scheme: A method for preparing an organic polypeptide enzyme activity promoter, comprising the following steps:

[0031] S1. Weigh and mince the pancreas of an animal and mix it evenly with 1 / 6-1 / 3 of the total amount of the adhesion promoter, then add the loaded porous filler and mix and stir evenly, and treat at 35-37° C. for 10-15 minutes to obtain a primary mixture;

[0032] S2, weighing animal brain, soybeans, and peanuts, chopping them, mixing and stirring them evenly to prepare a mixture;

[0033] S3, mixing the primary mixed material with soybean hulls and stirring evenly, then adding the mixed material and the remaining adhesion promoter, continuing to stir evenly, and granulating to obtain a finished product.

[0034] By adopting the above technical scheme, the animal pancreas and the adhesion promoter are first mixed and then the loaded porous filler is added, and the bonding effect of the adhesion promoter is used to make the animal pancreas and the loaded porous filler bonded, thereby promoting the contact and reaction of trypsin and tributyrin; then the animal pancreas is contacted with soybean shells, and the hydrophilic effect of sodium alginate on the surface of the soybean shells and the supporting hydrophobic effect of the soybean shells themselves are used to make the soybean shells drain water, so that the crops have a better drought resistance effect in the early growth stage after sowing; and when the autumn harvest is approaching 3-4 months later, the hydrophobic effect of the loaded porous filler is combined with the hydrophobic effect of the soybean shells, so that the water in the soil is easy to flow out along the flow channel, and it is not easy to condense and gather in the soil, so that the crops have a better flood resistance effect; so as to ensure that the crops can grow well throughout the life cycle and increase the crop yield.

[0035] In summary, this application has the following beneficial effects:

[0036] 1. Animal brain, animal pancreas, loaded porous filler, soybean, soybean shell and peanut are combined to utilize the nutrients in animal brain, soybean and peanut to promote the root growth of crops. Calcium butyrate, water and carbon dioxide are generated by the reaction of animal pancreas and loaded porous filler. Calcium butyrate and soybean shell can form gel with hydrophilic effect, which promotes drought resistance in the early stage of crop growth. The porous, ventilated and loose effects of loaded porous filler, carbon dioxide and soybean shell are utilized to further promote root growth. With the growth of crops, the gel gradually disintegrates and the soil water retention network is gradually lost. When floods occur in the middle and late stages of crop growth, the hydrophobic effect of loaded porous filler and the hydrophobic effect of soybean shell are utilized to further prevent water from gathering and condensing near the soil root system and affecting the root system's absorption of oxygen, thereby ensuring the growth of crops.

[0037] 2. Phosphatidylserine solution, calcium carbonate particles, hot-melt propolis liquid and tributyrin liquid are combined, and the lipophilicity of the propolis membrane is used to attract and connect with the lipophilic group on the outside of the phosphatidylserine, so that the calcium carbonate powder can enter the internal pores of the multi-porous filler and be loaded in the pore structure more stably; with the entry of tributyrin, the lipophilicity of propolis and the lipophilicity of phosphatidylserine are combined with the lipophilic group of tributyrin to further improve the adhesion of tributyrin to the pores of the multi-porous filler, so that the hydrophilic group of tributyrin faces outward; in the early stage of crop growth, the hydrophilic and water-retaining effects are achieved, and in the middle and late stages of crop growth, the hydrophobic groups in the pores of the multi-porous filler are used to further promote water loss, and try to avoid water agglomeration in the soil, which affects the later growth of the crop.

[0038] 3. The combination of propolis, open-porous fillers and soybean shells can further drain the soil, improve soil permeability, ensure the oxygen content in the soil, and thus promote the growth of plant roots. DETAILED DESCRIPTION

[0039] The present application is further described in detail below in conjunction with embodiments.

[0040] Preparation Example of Calcium Carbonate Micropowder

[0041] Preparation Example 1: Calcium carbonate powder is prepared by the following method:

[0042] Weigh propolis and heat it to 68°C until it is completely hot-melted to obtain hot-melt propolis liquid; spray 0.3kg of the hot-melt propolis liquid evenly on the surface of 1kg of calcium carbonate particles, then dry and disperse them until they are not sticky to each other to obtain calcium carbonate micropowder; the particle size of the calcium carbonate micropowder is 100 mesh.

[0043] Preparation Example 2: Calcium carbonate powder is prepared by the following method:

[0044] Weigh propolis and heat it to 68°C until it is completely hot-melted to obtain hot-melt propolis liquid; spray 0.2kg of the hot-melt propolis liquid evenly on the surface of 1kg of calcium carbonate particles, then dry and disperse them until they are not sticky to each other to obtain calcium carbonate micropowder; the particle size of the calcium carbonate micropowder is 100 mesh.

[0045] Preparation Example 3: Calcium carbonate powder is prepared by the following method:

[0046] Weigh propolis and heat it to 68°C until it is completely hot-melted to obtain hot-melt propolis liquid; spray 0.4 kg of the hot-melt propolis liquid evenly on the surface of 1 kg of calcium carbonate particles, then dry and disperse them until they are not sticky to each other to obtain calcium carbonate micropowder; the particle size of the calcium carbonate micropowder is 100 mesh.

[0047] Preparation example of loaded porous filler

[0048] Preparation Example 4: The loaded porous filler is prepared by the following method:

[0049] 1 kg of multi-porous filler is weighed and placed in 8 kg of phosphatidylserine solution, ultrasonically dispersed at 20 kHz for 2 minutes, and then soaked for 5 minutes. The multi-porous filler consists of zeolite and perlite with a weight ratio of 1:2, the zeolite particle size is 18 mesh, and the perlite particle size is 18 mesh. The phosphatidylserine solution is prepared by placing phosphatidylserine in petroleum ether and stirring to dissolve it, so as to obtain a phosphatidylserine solution with a mass fraction of 5%; then 1 kg of calcium carbonate powder prepared in Preparation Example 1 is added, and ultrasonic dispersion is continued at 20 kHz for 3 minutes, and then the multi-porous filler is taken out and dried until the petroleum ether is completely volatilized to obtain a hydrophobically modified filler;

[0050] 1 kg of hydrophobic modified filler was placed in 5 kg of tributyrin liquid, the mass fraction of tributyrin liquid was 1%, the solvent was ethanol, stirred at a speed of 100 r / min for 1 min, then soaked for 3 min, and finally the hydrophobic modified filler was taken out and dried until the ethanol was completely volatilized to obtain a finished loaded porous filler.

[0051] Preparation Example 5: The loaded porous filler is prepared by the following method:

[0052] 1 kg of multi-porous filler is weighed and placed in 8 kg of phosphatidylserine solution, ultrasonically dispersed at 20 kHz for 2 minutes, and then soaked for 2 minutes. The multi-porous filler consists of zeolite and perlite with a weight ratio of 1:1, the zeolite particle size is 18 mesh, and the perlite particle size is 18 mesh. The phosphatidylserine solution is prepared by placing phosphatidylserine in petroleum ether and stirring to dissolve it, so as to obtain a phosphatidylserine solution with a mass fraction of 5%; then 1 kg of calcium carbonate powder prepared in Preparation Example 2 is added, and ultrasonic dispersion is continued at 20 kHz for 3 minutes, and then the multi-porous filler is taken out and dried until the petroleum ether is completely volatilized to obtain a hydrophobically modified filler;

[0053] 1 kg of hydrophobic modified filler was placed in 5 kg of tributyrin liquid, the mass fraction of tributyrin liquid was 1%, the solvent was ethanol, stirred at a speed of 100 r / min for 1 min, then soaked for 3 min, and finally the hydrophobic modified filler was taken out and dried until the ethanol was completely volatilized to obtain a finished loaded porous filler.

[0054] Preparation Example 6: The loaded porous filler is prepared by the following method:

[0055] 1 kg of multi-porous filler is weighed and placed in 8 kg of phosphatidylserine solution, ultrasonically dispersed at 20 kHz for 2 minutes, and then soaked for 4 minutes. The multi-porous filler consists of zeolite and perlite with a weight ratio of 1:3, the zeolite particle size is 18 mesh, and the perlite particle size is 18 mesh. The phosphatidylserine solution is prepared by stirring and dissolving phosphatidylserine in petroleum ether to obtain a phosphatidylserine solution with a mass fraction of 5%; then 1 kg of calcium carbonate powder prepared in Preparation Example 3 is added, and ultrasonic dispersion is continued at 20 kHz for 3 minutes. Then the multi-porous filler is taken out and dried until the petroleum ether is completely volatilized to obtain a hydrophobically modified filler;

[0056] 1 kg of hydrophobic modified filler was placed in 5 kg of tributyrin liquid, the mass fraction of tributyrin liquid was 1%, the solvent was ethanol, stirred at a speed of 100 r / min for 1 min, then soaked for 3 min, and finally the hydrophobic modified filler was taken out and dried until the ethanol was completely volatilized to obtain a finished loaded porous filler.

[0057] Preparation Example of Soybean Hulls

[0058] Preparation Example 7: Soybean hulls are prepared by the following method:

[0059] Weigh 1 kg of crushed soybean husks, spray 0.2 kg of sodium alginate solution evenly on the surface thereof, wherein the sodium alginate solution is a sodium alginate aqueous solution with a mass fraction of 0.8%, and dry and disperse the mixture until they are not adhered to each other to obtain finished soybean husks.

[0060] Example

[0061] Example 1: An organic polypeptide enzyme activity promoter:

[0062] 13kg of animal brain, 24kg of animal pancreas, 15kg of soybeans, 8kg of soybean shells, 15kg of peanuts, 15kg of loaded porous filler, and 12kg of adhesion promoter; the animal brain is pig brain; the animal pancreas is pig pancreas; the soybean shells are the soybean shells prepared in Preparation Example 7; the loaded porous filler is the loaded porous filler prepared in Preparation Example 4; the adhesion promoter is a 0.5% by mass sodium hydroxymethylcellulose aqueous solution; the preparation method is as follows:

[0063] S1. Weigh the animal pancreas and mince it at a speed of 120 r / min for 1 min, then mix it evenly with 3 kg of adhesion promoter, then mix the minced animal pancreas and the loaded porous filler evenly, and treat it at 37° C. for 12 min to prepare a primary mixture;

[0064] S2, weighing animal brain and chopping it at a speed of 120 r / min for 1 min, chopping soybeans at a speed of 360 r / min for 2 min, and chopping peanuts at a speed of 360 r / min for 2 min, mixing and stirring evenly to prepare a mixture;

[0065] S3. The primary mixed material and soybean hulls are mixed and stirred evenly, and then the mixed material and the remaining adhesion promoter are added, and the stirring is continued evenly, and the finished product is obtained by granulation, and the particle size of the finished product is 3 mm.

[0066] Embodiment 2: This embodiment differs from Embodiment 1 in that:

[0067] 10kg of animal brain, 15kg of animal pancreas, 10kg of soybean, 5kg of soybean shell, 10kg of peanut, 10kg of loaded porous filler, 6kg of adhesion promoter; the animal brain is bovine brain; the animal pancreas is bovine pancreas; the loaded porous filler is the loaded porous filler prepared in Preparation Example 5;

[0068] In the preparation method:

[0069] S1. Weigh the animal pancreas and chop it at a speed of 120r / min for 1 minute, then mix it evenly with 1kg of adhesion promoter, then mix the chopped animal pancreas and the loaded porous filler evenly, and treat it at 35°C for 15 minutes to prepare a primary mixture.

[0070] Embodiment 3: This embodiment differs from Embodiment 1 in that:

[0071] 15kg of animal brain, 30kg of animal pancreas, 18kg of soybean, 12kg of soybean shell, 18kg of peanut, 20kg of loaded porous filler, 15kg of adhesion promoter; the animal brain is rabbit brain; the loaded porous filler is the loaded porous filler prepared in Preparation Example 6;

[0072] In the preparation method:

[0073] S1. Weigh the animal pancreas and chop it at a speed of 120r / min for 1 minute, then mix it evenly with 5kg of adhesion promoter, then mix the chopped animal pancreas and the loaded porous filler evenly, and treat it at 36°C for 10 minutes to prepare a primary mixture.

[0074] Embodiment 4: This embodiment differs from Embodiment 1 in that:

[0075] The phosphatidylserine solution was replaced by an equal mass of KH-570 silane coupling agent in the raw material of the loaded porous filler.

[0076] Embodiment 5: This embodiment differs from Embodiment 1 in that:

[0077] The calcium carbonate micropowder in the loaded porous filler is not treated with hot-melt propolis liquid, but is only ordinary commercially available calcium carbonate micropowder.

[0078] Embodiment 6: This embodiment differs from Embodiment 1 in that:

[0079] The soybean hulls in the accelerator were not treated with sodium alginate solution.

[0080] Comparative Example

[0081] Comparative Example 1: The difference between this comparative example and Example 1 is that:

[0082] During the preparation of loaded porous fillers:

[0083] 1 kg of the open porous filler was weighed and placed in 8 kg of sodium alginate solution, ultrasonically dispersed for 2 minutes at 20 kHz, and then soaked for 5 minutes. The open porous filler consisted of zeolite and perlite in a weight ratio of 1:2, the zeolite particle size was 18 mesh, the perlite particle size was 18 mesh, and the sodium alginate solution was a sodium alginate aqueous solution with a mass fraction of 0.5%. After drying, a finished loaded porous filler was obtained.

[0084] Comparative Example 2: The difference between this comparative example and Example 1 is that:

[0085] During the preparation of loaded porous fillers:

[0086] 1 kg of multi-porous filler is weighed and placed in 8 kg of phosphatidylserine solution, ultrasonically dispersed for 2 minutes under 20 kHz conditions, and then soaked for 5 minutes. The multi-porous filler consists of zeolite and perlite with a weight ratio of 1:2, the zeolite particle size is 18 mesh, and the perlite particle size is 18 mesh. The phosphatidylserine solution is prepared by placing phosphatidylserine in petroleum ether and stirring to dissolve it, so as to obtain a phosphatidylserine solution with a mass fraction of 5%; and the finished loaded porous filler is obtained by drying until the petroleum ether is completely volatilized.

[0087] Comparative Example 3: The difference between this comparative example and Example 1 is that:

[0088] The phosphatidylserine solution and tributyrin solution were replaced by KH-570 silane coupling agent of equal mass in the raw material of the loaded porous filler.

[0089] Comparative Example 4: The difference between this comparative example and Example 1 is that:

[0090] The open porous filler is replaced by an equal mass of silicon dioxide in the raw material.

[0091] Comparative Example 5: The difference between this comparative example and Example 1 is that:

[0092] No adhesion promoter is added to the raw materials.

[0093] Comparative Example 6: The difference between this comparative example and Example 1 is that:

[0094] The soybean hulls were replaced with peanuts of equal mass in the raw materials.

[0095] Performance testing

[0096] 1. Soil bulk density test

[0097] The accelerators were prepared by the methods of Examples 1-3 and Comparative Example 1, respectively. Corn was cultivated with the accelerators. The straw in the field was cut and returned to the field. 100 kg of accelerator was applied per mu of land. The corn was sown 10 days later. The corn varieties were all the same. The bulk density at a soil depth of 15 cm was tested before sowing, which was recorded as the initial bulk density. 45 days after sowing, the soil bulk density at a soil depth of 15 cm was tested, which was recorded as bulk density A. The average precipitation was 400 mm.

[0098] 2. Root rot detection

[0099] The accelerators were prepared by the methods of Examples 1-3 and Comparative Examples 1-2, respectively. Corn was cultivated with the accelerators. The straw in the field was cut and returned to the field. 100 kg of accelerator was applied per mu of land. The corn was sown 10 days later. The corn varieties were all the same. The average precipitation was 400 mm 1-40 days after sowing, 500 mm 40-80 days, and 800 mm 80-100 days. The corn was harvested at 120 days, and the root rot was observed. 100 corns were selected to record the degree of rot, and the average value was recorded.

[0100] 3. Plant height detection

[0101] The accelerators were prepared by the methods of Examples 1-6 and Comparative Examples 1-6, respectively. Corn was cultivated with the accelerators. The straw in the field was cut and returned to the field. 100 kg of accelerator was applied per mu of land. The corn was sown 10 days later. The corn varieties were all the same. The average precipitation was 300 mm 0-20 days after sowing, no precipitation 20-45 days, the average precipitation 480 mm 45 days-90 days, and the average precipitation 900 mm between the 90th and 100th days. Then, drainage and diversion were carried out. After the water was completely diverted out for 5 days, the corn was harvested on the 120th day and the average plant height was recorded.

[0102] 4. Yield detection

[0103] The accelerators were prepared by the methods of Examples 1-6 and Comparative Examples 1-6, respectively. Corn was cultivated with the accelerators. The straw in the field was cut and returned to the field. 100 kg of accelerator was applied per mu of land. The corn was sown 10 days later. The corn varieties were all the same. The average precipitation was 300 mm 0-20 days after sowing, no precipitation 20-45 days, the average precipitation 480 mm 45 days-90 days, and the average precipitation 900 mm between the 90th and 100th days. Then, drainage and diversion were carried out. After the water was completely diverted out for 5 days, the corn was harvested on the 120th day and the corn yield was recorded.

[0104] The above experimental fields were selected from Changchun City, Jilin Province.

[0105] Table 1 Performance test table

[0106]

[0107]

[0108] It can be seen from Examples 1-3 and Table 1 that the finished product prepared in the present application can reduce soil bulk density and avoid root rot as much as possible under flood conditions, while enabling crops to grow well under conditions of drought in the early stage and flood in the later stage, thereby enabling crops to have a higher yield.

[0109] Combining Example 1 and Examples 4-6 and Table 1, it can be seen that in Example 4, the phosphatidylserine solution is replaced with an equal mass of KH-570 silane coupling agent in the raw material of the porous filler. Compared with Example 1, the plant height of the crop in Example 4 is lower than that in Example 1, and the yield is lower than that in Example 1; this indicates that the KH-570 silane coupling agent only has a hydrophobic effect, and has no hydrophilic group to provide calcium carbonate powder and tributyrin to bond in the pores, thereby affecting the loading amount of the pores for calcium carbonate powder and tributyrin, and affecting the growth of the crop.

[0110] The calcium carbonate powder in the porous filler loaded in Example 5 has not been treated with hot-melt propolis liquid. Compared with Example 1, the crop plant height of Example 5 is lower than that of Example 1, and the yield is lower than that of Example 1. This indicates that the calcium carbonate powder is coated with propolis, and the lipophilicity of propolis can promote the more stable attachment of the calcium carbonate powder in the pores, thereby ensuring the reaction of the calcium carbonate powder with the generated butyric acid, and utilizing the inhibitory effect of calcium butyrate on harmful bacteria to improve the air permeability of the soil, thereby further promoting the growth of crops.

[0111] The soybean shells in the promoter of Example 6 are not treated with sodium alginate solution. Compared with Example 1, the plant height of the crop in Example 6 is lower than that in Example 1, and the yield is lower than that in Example 1; this indicates that the soybean shells themselves are oleophobic, while the sodium alginate solution is hydrophilic. In the early stage of crop growth, the hydrophilicity of the gel formed by sodium alginate and calcium ions is utilized to ensure crop growth in the drought stage. In the later stage, sodium alginate is degraded by saprophytes, and the oleophobicity of the soybean shells comes into play. When encountering flood disasters, the hydrophobic effect of phosphatidylserine in the pores of the loaded porous filler is combined with the hydrophobic effect of the soybean shells to avoid moisture accumulation near the soil root system as much as possible, thereby affecting the later growth of the crop.

[0112] Combining Example 1 and Comparative Examples 1-6 and Table 1, it can be seen that the open-porous filler of Comparative Example 1 is treated with a sodium alginate solution. Compared with Example 1, the soil bulk density of Comparative Example 1 increases after precipitation, the degree of crop root decay is greater than that of Example 1, the crop plant height is less than that of Example 1, and the yield is less than that of Example 1; this indicates that the hydrophilicity of sodium alginate can easily form a hydrophilic water-absorbing network in the soil. Even as time goes on, the sodium alginate in the pores of the open-porous filler is gradually released to form a network with moisture in the soil. When floods occur, moisture accumulates in the soil, which can easily affect the absorption of oxygen by the crop roots, thereby affecting the growth of the crops.

[0113] During the preparation of the loaded open-pored filler in Comparative Example 2, the calcium carbonate powder and tributyrin liquid were not treated. Compared with Example 1, the degree of root rot of the crops in Comparative Example 2 was greater than that in Example 1, the crop plant height was less than that in Example 1, and the yield was less than that in Example 1; this indicates that the butyric acid generated by the reaction of pancreatic lipase and tributyrin first reacted with calcium carbonate, and the reaction product butyric acid was slowly released in the multi-porous filler, and the pH value was adjusted by calcium butyrate to promote the growth and reproduction of beneficial bacteria in the soil, so as to promote the growth of crops; the network formed by the calcium ions in calcium butyrate and water in the early stage of crop growth further ensures the growth of crops in drought periods; and as time goes on, during the flood period, the hydrophobic groups in the multi-porous filler can prevent the accumulation of water in the soil from affecting the growth of crops.

[0114] In Comparative Example 3, the phosphatidylserine solution and the tributyrin solution were replaced with KH-570 silane coupling agent of equal mass in the raw materials of the loaded porous filler. Compared with Example 1, the plant height of the crop in Comparative Example 3 was lower than that in Example 1, and the yield was lower than that in Example 1, indicating that there was no hydrophilic group on the surface of the loaded porous filler, which could easily affect the growth of the crop during the drought stage in the early growth stage of the crop.

[0115] In Comparative Example 4, the open-porous filler was replaced with an equal mass of silica in the raw materials. Compared with Example 1, the crop plant height and yield of Comparative Example 4 were smaller than those of Example 1, indicating that silica does not have the open-porous structure of zeolite and perlite, is easy to affect the porosity in the soil, and cannot ensure good growth of crops during droughts and floods.

[0116] In Comparative Example 5, no adhesion promoter was added to the raw materials, and the soil bulk density increased after precipitation. Compared with Example 1, the crop plant height of Comparative Example 5 was smaller than that of Example 1, and the yield was smaller than that of Example 1. This indicates that the adhesion promoter can facilitate the reaction of pancreatic lipase with tributyrin, thereby ensuring the reaction of butyric acid with calcium carbonate, and ultimately enabling the crops to still grow well under initial drought and later flood conditions.

[0117] In Comparative Example 6, soybean shells were replaced with peanuts of equal mass in the raw materials. Compared with Example 1, the plant height of the crops in Comparative Example 6 was smaller than that in Example 1, and the yield was smaller than that in Example 1. This indicates that the combination of soybean shells and loaded porous fillers can promote water loss during floods, thereby ensuring the growth of crops.

[0118] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An organic polypeptide enzyme activity promoter, characterized in that: The invention is prepared from the following raw materials in parts by weight: 10-15 parts of animal brain, 15-30 parts of animal pancreas, 10-18 parts of soybean, 5-12 parts of soybean shell, 10-18 parts of peanut, 10-20 parts of loaded porous filler, and 6-15 parts of adhesion promoter; the loaded porous filler is prepared by sequentially loading calcium carbonate powder and tributyrin on a multi-porous filler after hydrophobic modification; the soybean shell is prepared by treating the surface of crushed soybean shell with a sodium alginate solution; the adhesion promoter is a sodium hydroxymethylcellulose aqueous solution; The loaded porous filler is prepared by the following method: Weighing a porous filler, placing it in a phosphatidylserine solution, dispersing and stirring, then soaking it for 2-5 minutes, adding calcium carbonate powder, dispersing it, then taking out the porous filler, and drying it to obtain a hydrophobic modified filler; the porous filler is composed of zeolite and perlite in a weight ratio of 1:1-3; the calcium carbonate powder is composed of calcium carbonate particles and hot-melt propolis liquid in a mass ratio of 1:0.2-0.4; The hydrophobically modified filler is placed in a tributyrin solution for dispersion and soaking, then taken out and dried to obtain a finished loaded porous filler.

2. The organic polypeptide enzyme activity promoter according to claim 1, characterized in that: The animal pancreas is one or more of a pig pancreas and a cow pancreas.

3. The organic polypeptide enzyme activity promoter according to claim 1, characterized in that: The animal brain is one or more of pig brain, cow brain and rabbit brain.

4. The method for preparing an organic polypeptide enzyme activity promoter according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Weigh and mince the pancreas of an animal and mix it evenly with 1 / 6-1 / 3 of the total mass of the adhesion promoter, then add the loaded porous filler and mix and stir evenly, and treat at 35-37° C. for 10-15 minutes to obtain a primary mixture; S2, weighing animal brain, soybeans, and peanuts, chopping them, mixing and stirring them evenly to prepare a mixture; S3, mixing the primary mixed material with the soybean hulls and stirring them uniformly, then adding the mixed material and the remaining adhesion promoter, continuing to stir them uniformly, and granulating to obtain a finished product.

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