In vitro analytical diagnostic test device and cartridge valve control assembly

Abstract

The application relates to an in-vitro analysis diagnosis detection device and a cartridge valve control assembly. When a first driving mechanism drives a first air pipe and a second air pipe to move to a first position, a first joint and a third joint are respectively used for communicating with a first air hole and a second air hole of a reagent cartridge. When an air path control assembly works in a first working state, a second joint is controlled to communicate with a pressure control mechanism, and a fourth joint communicates with an atmospheric environment. When the air path control assembly works in a second working state, the second joint communicates with the atmospheric environment, and the fourth joint communicates with the pressure control mechanism. Through switching actions of the air path control assembly, the first air hole of the reagent cartridge can communicate with the pressure control mechanism through the first air pipe, and the second air hole of the reagent cartridge can communicate with the pressure control mechanism through the second air pipe. Power can be provided according to process requirements of the reagent cartridge to push sample liquid to transfer between cavities. Meanwhile, the overall structure of the cartridge valve control assembly is relatively simplified.

Description

Technical Field

[0001] This invention relates to the field of in vitro analytical diagnostics technology, and in particular to an in vitro analytical diagnostic testing device and a cartridge valve control assembly. Background Technology

[0002] This integrated point-of-care testing (POCT) instrument system for rapid pathogen detection based on PCR (polymerase chain reaction) technology is suitable for analyzing various sample types, including nasopharyngeal swabs, sputum, urine, stool, cervical / vaginal swabs, blood, cerebrospinal fluid, skin, and wound swabs. The system automatically analyzes samples by adding them to disposable reagent cartridges, placing the cartridges into the instrument, and performing nucleic acid amplification, detection, calculation, and result printing. Even untrained personnel can easily operate the system and obtain reliable and accurate results. The instrument and reagents are suitable for single or multiplex detection of pathogen DNA or RNA that infects the human body.

[0003] Nucleic acid extraction using centrifugation column methods or magnetic bead methods in the biotechnology field generally requires four steps: lysis, binding, washing, and elution, plus subsequent steps such as nucleic acid hybridization, polymerase chain reaction, and molecular detection. Molecular detection technology is developing towards accuracy, convenience, sensitivity, automation, and integration. However, due to the inherent complexity of molecular detection technology, fully automated instrument platforms from sample to result are extremely rare, and instrument systems for automatically transferring sample liquids, such as from the sample chamber to the pretreatment chamber, the pretreatment chamber to the mixing chamber, or the mixing chamber to the PCR chamber, are structurally complex. Summary of the Invention

[0004] Therefore, it is necessary to overcome the shortcomings of the existing technology and provide an in vitro analysis and diagnostic testing device and a cartridge valve control component that can facilitate the transfer of sample liquid between chambers while simplifying the structure.

[0005] The technical solution is as follows: A cartridge valve control assembly, comprising: a bracket for mounting a reagent cartridge; a first clamping assembly, the first clamping assembly comprising a first driving mechanism, a first air tube, and a second air tube, the first driving mechanism being mounted on the bracket, the first driving mechanism driving the first air tube and the second air tube to move, one end of the first air tube having a first connector, the other end of the first air tube having a second connector, one end of the second air tube having a third connector, and the other end of the second air tube having a fourth connector, wherein when the first driving mechanism drives the first air tube and the second air tube to a first position, the first... A connector and a third connector are respectively used to connect with the first and second air vents of the reagent cartridge; a gas path control component and a pressure control mechanism are provided, the gas path control component being connected to the second connector, the fourth connector, and the pressure control mechanism; the gas path control component has a first working state and a second working state; when the gas path control component is working in the first working state, it is used to control the second connector to connect with the pressure control mechanism, and the fourth connector to connect with the atmospheric environment; when the gas path control component is working in the second working state, it is used to control the second connector to connect with the atmospheric environment, and the fourth connector to connect with the pressure control mechanism.

[0006] The aforementioned cartridge valve control assembly, when it is necessary to test the sample liquid inside the reagent cartridge, inserts the reagent cartridge into the support. The first drive mechanism moves the first and second air tubes to a first position, so that the first connector connects to the first air port and the third connector connects to the second air port. When the gas path control assembly is working in the first working state, the first air port connects to the pressure control mechanism through the first air tube, and the second air port connects to the atmospheric environment through the second air tube. The suction force provided by the pressure control mechanism acts on the reagent cartridge, causing the sample liquid inside the reagent cartridge to transfer between chambers. Similarly, when the gas path control assembly is working in the second working state, the second air port connects to the pressure control mechanism through the second air tube, and the first air port connects to the atmospheric environment through the first air tube. The suction force provided by the pressure control mechanism acts on the reagent cartridge, causing the sample liquid inside the reagent cartridge to transfer between chambers. In this way, by switching the gas path control component, the first air hole of the reagent cartridge can be connected to the pressure control mechanism through the first air tube, and the second air hole of the reagent cartridge can be connected to the pressure control mechanism through the second air tube. It can provide power to drive the sample liquid to transfer between chambers according to the process requirements of the reagent cartridge. At the same time, the overall structure of the cartridge valve control component is relatively simple.

[0007] In one embodiment, both the first connector and the third connector are vacuum suction cups.

[0008] In one embodiment, the first clamping assembly further includes a first mounting plate; the first air pipe and the second air pipe are both disposed on the first mounting plate; the first driving mechanism is connected to the first mounting plate, and the first driving mechanism drives the first mounting plate to move.

[0009] In one embodiment, the first clamping assembly further includes a first elastic element; the first air tube is connected to the first mounting plate via the first elastic element.

[0010] In one embodiment, the first mounting plate is provided with a first guide hole, the first air tube is movably disposed in the first guide hole, the wall of the first guide hole is provided with a first step, the outer wall of the first air tube is provided with a second step, and the two ends of the first elastic member respectively abut against the first step and the second step.

[0011] In one embodiment, the first clamping assembly further includes at least one first clamping member; at least one first clamping member is mounted on the first mounting plate; the first clamping member is used to clamp or release the first valve of the reagent cartridge.

[0012] In one embodiment, the cartridge valve control assembly further includes a second clamping assembly; the second clamping assembly includes a second drive mechanism, a second mounting plate, and at least one second clamping member; the second drive mechanism is disposed on the bracket, and at least one second clamping member is mounted on the second mounting plate; the second drive mechanism is connected to the second mounting plate and is used to drive the second mounting plate to move closer to or away from the bracket.

[0013] In one embodiment, the cartridge valve control assembly further includes a slide rail assembly; the slide rail assembly includes a fixed frame mounted on the bracket, a first slide rail and a second slide rail disposed on the fixed frame, a first slider slidably disposed on the first slide rail, a second slider slidably disposed on the second slide rail, a first connecting plate connected to the first slider, and a second connecting plate connected to the second slider; the first connecting plate is connected to the first mounting plate, and the second connecting plate is connected to the second mounting plate.

[0014] In one embodiment, the cartridge valve control assembly further includes a first sensor and a first trigger that cooperates with the first sensor; the first sensor is mounted on the bracket, and the first trigger is mounted on the first mounting plate or the first connecting plate; the cartridge valve control assembly further includes a second sensor and a second trigger that cooperates with the second sensor; the second sensor is mounted on the bracket, and the second trigger is mounted on the second mounting plate or the second connecting plate.

[0015] In one embodiment, the first clamping assembly further includes at least one second elastic element mounted on the first mounting plate, wherein at least one first clamping element and at least one second elastic element are respectively disposed in a one-to-one correspondence, and the first clamping element is connected to the first mounting plate through the second elastic element.

[0016] The second clamping assembly further includes at least one third elastic element mounted on the second mounting plate. At least one second clamping element and at least one third elastic element are respectively arranged in a one-to-one correspondence. The second clamping element is connected to the second mounting plate through the third elastic element.

[0017] In one embodiment, the first mounting plate has a first receiving chamber and a second guide hole communicating with the first receiving chamber. The second elastic member is disposed in the first receiving chamber. One end of the first clamping member is located inside the first receiving chamber and is connected to the second elastic member. The first clamping member is movably disposed in the second guide hole. The other end of the first clamping member extends out of the second guide hole and is used to clamp or release the first valve of the reagent card box.

[0018] The second mounting plate has a second receiving chamber and a third guide hole communicating with the second receiving chamber. The third elastic member is located in the second receiving chamber. One end of the second clamping member is located inside the second receiving chamber and is connected to the third elastic member. The second clamping member is movably disposed in the third guide hole. The other end of the second clamping member extends out of the third guide hole and is used to clamp or release the second valve of the reagent card box.

[0019] An in vitro analytical diagnostic testing device, the in vitro analytical diagnostic testing device including the aforementioned cartridge valve control component.

[0020] In the aforementioned in vitro analytical diagnostic testing device, when it is necessary to test the sample liquid inside the reagent cartridge, the reagent cartridge is inserted into the support. The first driving mechanism moves the first and second air tubes to a first position, so that the first connector connects to the first air port and the third connector connects to the second air port. When the gas path control component is working in the first working state, the first air port connects to the pressure control mechanism through the first air tube, and the second air port connects to the atmospheric environment through the second air tube. The suction force provided by the pressure control mechanism acts on the reagent cartridge, causing the sample liquid inside the reagent cartridge to transfer between chambers. Similarly, when the gas path control component is working in the second working state, the second air port connects to the pressure control mechanism through the second air tube, and the first air port connects to the atmospheric environment through the first air tube. The suction force provided by the pressure control mechanism acts on the reagent cartridge, causing the sample liquid inside the reagent cartridge to transfer between chambers. In this way, by switching the gas path control component, the first air hole of the reagent cartridge can be connected to the pressure control mechanism through the first air tube, and the second air hole of the reagent cartridge can be connected to the pressure control mechanism through the second air tube. It can provide power to drive the sample liquid to transfer between chambers according to the process requirements of the reagent cartridge. At the same time, the overall structure of the cartridge valve control component is relatively simple. Attached Figure Description

[0021] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a schematic diagram of the card box valve control assembly according to one embodiment of the present invention;

[0024] Figure 2 This is a schematic diagram of the card box valve control assembly according to another embodiment of the present invention;

[0025] Figure 3 This is a schematic diagram of the cartridge valve control assembly of an embodiment of the present invention, showing the hidden air path control assembly and pressure control mechanism from one perspective.

[0026] Figure 4 This is a schematic diagram of the cartridge valve control assembly of an embodiment of the present invention, showing the hidden air path control assembly and pressure control mechanism from another perspective.

[0027] Figure 5 This is a schematic diagram of the structure of a reagent cartridge according to an embodiment of the present invention;

[0028] Figure 6 This is a structural view of the first mounting plate according to an embodiment of the present invention;

[0029] Figure 7 This is another structural view of the first mounting plate according to an embodiment of the present invention;

[0030] Figure 8 This is another structural view of the first mounting plate according to an embodiment of the present invention;

[0031] Figure 9 for Figure 8 Sectional view at BB;

[0032] Figure 10 This is a structural view of the second mounting plate according to an embodiment of the present invention;

[0033] Figure 11 This is a structural view of a slide rail assembly according to an embodiment of the present invention;

[0034] Figure 12 This is another structural view of a slide rail assembly according to an embodiment of the present invention;

[0035] Figure 13 This is a structural view of the bracket of a cartridge valve control assembly according to an embodiment of the present invention;

[0036] Figure 14 This is another structural view of the bracket of the cartridge valve control assembly according to an embodiment of the present invention;

[0037] Figure 15 for Figure 14 The diagram shows the enlarged structure at point A after the guide plate is hidden.

[0038] 10. Bracket; 11. Support plate; 111. First recess; 112. Inlet / outlet; 113. Activity chamber; 12. Guide plate; 121. First through hole; 122. Second through hole; 20. First clamping assembly; 21. First drive mechanism; 22. First air pipe; 221. First connector; 222. Second connector; 223. Second step; 23. Second air pipe; 231. Third connector; 232. Fourth connector; 24. First mounting plate; 241. First receiving chamber; 242. First guide through hole; 243. Second guide through hole; 244. First step; 25. First elastic element; 26. First clamping element; 27. Second elastic element; 31. Gas path control assembly; 32. Pressure control mechanism; 40. Reagent card holder; 41. First air hole; 42. 43. Second vent; 44. Protrusion; 45. First valve; 46. Second valve; 47. Sample chamber; 48. Pretreatment chamber; 49. Mixing chamber; 50. PCR chamber; 51. Limiting baffle; 51. Blocking part; 51. Guide edge; 52. Rotating component; 53. Fourth elastic component; 60. Second clamping assembly; 61. Second drive mechanism; 62. Second clamping component; 63. Second mounting plate; 70. Slide rail assembly; 71. Fixing frame; 72. First slide rail; 73. Second slide rail; 74. First slider; 75. Second slider; 76. First connecting plate; 77. Second connecting plate; 81. First sensor; 82. First trigger; 83. Second sensor; 84. Second trigger; 91. Pyrolysis assembly; 92. Magnetic mixing assembly. Detailed Implementation

[0039] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0040] See Figures 1 to 9 , Figure 1 and Figure 2 The diagrams illustrate the structure of the cartridge valve control assembly from two different perspectives in one embodiment of the present invention. Figure 3 and Figure 4 The diagrams shown are two different viewpoints illustrating the structure of the cartridge valve control assembly in one embodiment of the present invention, after concealing the pneumatic control assembly 31 and the pressure control mechanism 32. Figure 5 A schematic diagram of the structure of a reagent cartridge 40 according to one embodiment is shown. Figures 6 to 8 Three different viewpoint structural diagrams of the first mounting plate 24 according to an embodiment of the present invention are shown respectively. Figure 9 It shows Figure 8 Cross-sectional view at AA. An embodiment of the present invention provides a cartridge valve control assembly, which includes a bracket 10, a first clamping assembly 20, a gas path control assembly 31, and a pressure control mechanism 32. The bracket 10 is used to mount a reagent cartridge 40. The first clamping assembly 20 includes a first driving mechanism 21, a first gas pipe 22, and a second gas pipe 23. The first driving mechanism 21 is disposed on the bracket 10 and drives the first gas pipe 22 and the second gas pipe 23 to move. One end of the first gas pipe 22 is provided with a first connector 221, and the other end of the first gas pipe 22 is provided with a second connector 222. One end of the second gas pipe 23 is provided with a third connector 231, and the other end of the second gas pipe 23 is provided with a fourth connector 232. When the first driving mechanism 21 drives the first gas pipe 22 and the second gas pipe 23 to a first position, the first connector 221 and the third connector 231 are respectively used to connect with the first air hole 41 and the second air hole 42 of the reagent cartridge 40. The gas path control component 31 is connected to the second connector 222, the fourth connector 232, and the pressure control mechanism 32, respectively.

[0041] The gas path control component 31 has a first working state and a second working state. When the gas path control component 31 is working in the first working state, it controls the second connector 222 to connect with the pressure control mechanism 32 and the fourth connector 232 to connect with the atmospheric environment. When the gas path control component 31 is working in the second working state, it controls the second connector 222 to connect with the atmospheric environment and the fourth connector 232 to connect with the pressure control mechanism 32.

[0042] When the sample liquid in the reagent cartridge 40 needs to be tested, the reagent cartridge 40 is inserted into the bracket 10. The first drive mechanism 21 moves the first air tube 22 and the second air tube 23 to a first position, so that the first connector 221 is connected to the first air port 41 and the third connector 231 is connected to the second air port 42. When the gas path control component 31 is working in the first working state, the first air port 41 is connected to the pressure control mechanism 32 through the first air tube 22, and the second air port 42 is connected to the second air tube 231 through the second air tube 232. The trachea 23 is connected to the atmospheric environment. The suction force provided by the pressure control mechanism 32 acts on the reagent cartridge 40, causing the sample liquid inside the reagent cartridge 40 to transfer between chambers. Similarly, when the gas path control component 31 is working in the second working state, the second vent 42 is connected to the pressure control mechanism 32 through the second trachea 23, and the first vent 41 is connected to the atmospheric environment through the first trachea 22. The suction force provided by the pressure control mechanism 32 acts on the reagent cartridge 40, causing the sample liquid inside the reagent cartridge 40 to transfer between chambers. In this way, by switching the gas path control component 31, the first vent 41 of the reagent cartridge 40 can be connected to the pressure control mechanism 32 through the first trachea 22, and the second vent 42 of the reagent cartridge 40 can be connected to the pressure control mechanism 32 through the second trachea 23. This provides power to drive the sample liquid to transfer between chambers according to the process requirements of the reagent cartridge 40, while simplifying the overall structure of the cartridge valve control component.

[0043] It should be noted that the reagent card box 40 in this embodiment has at least two chambers, a flow passage, a first vent 41 and a second vent 42. The at least two chambers are connected through the flow passage. The first vent 41 and the second vent 42 are both connected to the flow passage. The specific structure of the flow passage is not limited here and can be set according to actual needs.

[0044] Please see Figure 5 Specifically, at least two chambers are included, for example, a sample chamber 46, a pretreatment chamber 47, a mixing chamber 48, and a PCR chamber 49; other functional chambers are also possible. The specific number of sample chambers 46, pretreatment chambers 47, mixing chambers 48, and PCR chambers 49 is not limited and can be set according to actual needs. In this embodiment, there is one sample chamber 46 for containing sample liquid; one pretreatment chamber 47 for treating the sample liquid, for example, by heating and lysing; two mixing chambers 48, for example, for mixing the sample liquid with lyophilized reagents; and two PCR chambers 49, for example, for performing polymerase chain reaction (PCR) and molecular detection on the sample liquid.

[0045] Please see Figures 6 to 9In one embodiment, both the first connector 221 and the third connector 231 are vacuum suction cups. Thus, when the first connector 221 is connected to the first air hole 41, the vacuum suction cup completely covers the outside of the wall of the first air hole 41, ensuring a tight seal between the first connector 221 and the first air hole 41. Similarly, when the third connector 231 is connected to the second air hole 42, the vacuum suction cup completely covers the outside of the wall of the second air hole 42, ensuring a tight seal between the third connector 231 and the second air hole 42.

[0046] Please see Figure 1 and Figure 2 Furthermore, the pneumatic control assembly 31 includes, for example, a solenoid control valve (such as...). Figure 1 and Figure 2 The quantity shown in the diagram is, for example, two) and the pipeline ( Figure 1 and Figure 2 (All details are hidden and not shown). The electromagnetic control valve is connected to the second connector 222, the fourth connector 232, and the pressure control mechanism 32 via pipelines. The pressure control mechanism 32 is specifically a vacuum pump, a blower, a plunger pump, etc. The electromagnetic control valve has a first working state and a second working state. When the electromagnetic control valve switches working states, it enables the first vent 41 of the reagent card box 40 to connect to the pressure control mechanism 32 through the first air pipe 22, and enables the second vent 42 of the reagent card box 40 to connect to the pressure control mechanism 32 through the second air pipe 23. It should be noted that the number of electromagnetic control valves, the specific structure of the pipelines, and the placement of the electromagnetic control valves on the pipelines are not limited here. They can be set according to actual needs, as long as at least the above-mentioned first and second working states can be achieved. For example, there are two electromagnetic control valves. When one electromagnetic control valve is activated, it can connect the second connector 222 to the air pump through the pipeline, or connect the second connector 222 to the atmospheric environment through the pipeline. When the other electromagnetic control valve is activated simultaneously, it can connect the fourth connector 232 to the atmospheric environment through the pipeline, or connect the fourth connector 232 to the air pump through the pipeline.

[0047] In one embodiment, the first clamping assembly 20 further includes a first mounting plate 24. Both the first air pipe 22 and the second air pipe 23 are disposed on the first mounting plate 24. A first driving mechanism 21 is connected to the first mounting plate 24, and the first driving mechanism 21 drives the first mounting plate 24 to move. Thus, the first driving mechanism 21 drives the first mounting plate 24 to move, and the first mounting plate 24 drives the first air pipe 22 and the second air pipe 23 to move synchronously, thereby simultaneously achieving the connection between the first connector 221 and the first air hole 41, and the connection between the third connector 231 and the second air hole 42.

[0048] In one embodiment, the first clamping assembly 20 further includes a first elastic element 25. The first air tube 22 is connected to the first mounting plate 24 via the first elastic element 25. Thus, during the process of the first connector 221 of the first air tube 22 connecting with the first air hole 41 of the reagent card box 40 under the push of the first driving mechanism 21, the first elastic element 25 acts as a buffer to prevent the first connector 221 from damaging the reagent card box 40.

[0049] In one embodiment, a first guide hole 242 is provided on the first mounting plate 24, a first air tube 22 is movably disposed in the first guide hole 242, a first step 244 is provided on the wall of the first guide hole 242, a second step 223 is provided on the outer wall of the first air tube 22, and the two ends of the first elastic member 25 respectively abut against the first step 244 and the second step 223.

[0050] Specifically, the first elastic element 25 is a spring sleeved on the outer wall of the trachea, with the two ends of the spring respectively contacting the first step 244 and the second step 223.

[0051] It should be noted that the installation method of the second air tube 23 on the first mounting plate 24 is similar to that of the first air tube 22, and will not be described in detail here.

[0052] Please see Figure 3 and Figure 4 , Figures 13 to 15Furthermore, the cartridge valve control assembly also includes a limiting baffle 50 and a fourth elastic element 53. The bracket 10 includes a support plate 11 and a guide plate 12. A first recess 111 is provided on one side of the support plate 11, which is adapted to fit the reagent cartridge 40. The guide plate 12 is disposed on one side of the support plate 11 and is used to abut against the side of the reagent cartridge 40 inserted into the first recess 111. One side wall of the first recess 111 has an inlet / outlet 112, and the other side wall of the first recess 111 has an active chamber 113. The active chamber 113 communicates with the first recess 111. The limiting baffle 50 is movably disposed within the active chamber 113, and the limiting baffle 50 is connected to the wall of the active chamber 113 via the fourth elastic element 53. The limiting baffle 50 has a blocking part 51. The blocking part 51 is used to abut against the reagent cartridge 40 inserted into the first recess 111. Thus, when the reagent cartridge 40 needs to be tested, the reagent cartridge 40 enters the first recess 111 through the inlet / outlet 112 along the guide plate 12. The guide plate 12 guides the reagent cartridge 40, and after entering the first recess 111, the reagent cartridge 40's position is relatively stable under the action of the guide plate 12 and the support plate 11. This ensures a relatively accurate relative position of the reagent cartridge 40, facilitating its alignment with the first clamping member 61, the second clamping member 62, and the first connector 221. The components include a second connector 222, a third connector 231, and a fourth connector 232. Furthermore, under the elastic force of the fourth elastic element 53, the blocking part 51 of the limiting baffle 50 presses against the reagent cartridge 40 with a certain pressure, preventing the reagent cartridge 40 from easily detaching from the inlet / outlet 112. This ensures the reagent cartridge 40 is securely mounted on the cartridge valve control assembly, guaranteeing the accuracy of the test results from the reagent cartridge 40. Simultaneously, the overall structure of the cartridge valve control assembly is simple and reliable.

[0053] In addition, after the analysis and testing operation of the reagent cartridge 40 is completed, the drive limit baffle 50 moves to separate the blocking part 51 from the reagent cartridge 40, and the reagent cartridge 40 is taken out through the inlet and outlet 112.

[0054] It should be noted that the "blocking part 51" can be "a part of the limiting baffle 50", that is, the "blocking part 51" and "other parts of the limiting baffle 50" are integrally molded; or it can be a separate component that can be separated from "other parts of the limiting baffle 50", that is, the "blocking part 51" can be manufactured independently and then combined with "other parts of the limiting baffle 50" to form a whole. Figure 15 As shown, in one embodiment, the "blocking part 51" is integrally molded as part of the "limiting baffle 50".

[0055] Please see Figure 14 and Figure 15Furthermore, one end of the limiting baffle 50 is rotatably connected to the wall of the movable chamber 113 via a rotating member 52. The other end of the limiting baffle 50 is connected to the wall of the movable chamber 113 via a fourth elastic member 53, and a blocking part 51 is also provided at the other end of the limiting baffle 50.

[0056] Please see Figure 5 , Figure 14 and Figure 15 In one embodiment, the blocking part 51 is provided with a guide edge 511, which is inclined towards the inlet / outlet 112. Furthermore, the inclination angle of the guide edge 511 relative to the direction in which the reagent cartridge 40 enters / exits the first recess 111 is 'a', where 'a' is 30° to 60°. Of course, 'a' can be any angle from 0° to 90°, and is not limited here; it is set according to actual needs. When the reagent cartridge 40 is inserted into the first recess 111 through the inlet / outlet 112, the protrusion 43 of the reagent cartridge 40 contacts the guide edge 511 and gradually enters the first recess 111 under the guidance of the guide edge 511. Simultaneously, the guide edge 511 pushes and compresses the fourth elastic member 53. When the reagent cartridge 40 is in place, the blocking part 51, under the action of the fourth elastic member 53, resets and moves, then abuts against the protrusion 43 of the reagent cartridge 40, thereby effectively engaging and abutting the protrusion 43 of the reagent cartridge 40, preventing the reagent cartridge 40 from detaching outwards through the inlet / outlet 112.

[0057] It should be noted that the direction in which the reagent card holder 40 enters and exits the first recess 111 is as follows: Figure 15 As shown by the double-headed arrow S.

[0058] Please see Figure 3 , Figure 4 , Figures 6 to 8 , Figures 6 to 8 Three different perspective structural schematic diagrams of the first mounting plate 24 of one embodiment are shown. In one embodiment, the first clamping assembly 20 further includes at least one first clamping member 26. At least one first clamping member 26 is mounted on the first mounting plate 24. The first clamping member 26 is used to clamp or release the first valve 44 of the reagent card holder 40. Specifically, the first clamping member 26 is, for example, a pin, a rod, etc., as long as it can push the first valve 44 to close the first valve 44, or release the first valve 44 to open the first valve 44. The size of the contact end face of the first clamping member 26 is set accordingly to the size of the first valve 44.

[0059] Please see Figure 3 , Figure 4 and Figure 10In one embodiment, the cartridge valve control assembly further includes a second clamping assembly 60 disposed on the support plate 11. The second clamping assembly 60 includes a second drive mechanism 61, a second mounting plate 63, and at least one second clamping member 62. At least one second clamping member 62 is mounted on the second mounting plate 63. The second drive mechanism 61 is connected to the second mounting plate 63 and is used to drive the second mounting plate 63 to move closer to or further away from the bracket 10. Thus, the second drive mechanism 61 drives the second mounting plate 63 to move, the second mounting plate 63 drives the second clamping member 62 to move, and the second clamping member 62 can clamp or release the second valve 45 of the reagent cartridge 40. When the second clamping member 62 clamps the second valve 45, the second valve 45 is closed; when the second clamping member 62 releases the second valve 45, the second valve 45 is opened.

[0060] See Figures 6 to 10 In one embodiment, the number of first clamping members 26 mounted on the first mounting plate 24 is not less than the number of first valves 44 of the reagent cartridge 40, and the number of second clamping members 62 mounted on the second mounting plate 63 is not less than the number of second valves 45 of the reagent cartridge 40. Thus, when the first driving mechanism 21 drives the first mounting plate 24 to move, it can open or close all the first valves 44 of the reagent cartridge 40; similarly, when the second driving mechanism 61 drives the second mounting plate 63 to move, it can open or close all the second valves 45 of the reagent cartridge 40. For example, in this embodiment, there are four first clamping members 26, three of which correspond one-to-one with the three first valves 44 of the reagent cartridge 40, and the other first clamping member 26 is also used to clamp or loosen the reagent cartridge 40, but it is not clamped at the position where the first valves 44 are located, thus ensuring a good clamping effect on the side of the reagent cartridge 40. In addition, in this embodiment, the number of second clamping members 62 is, for example, four, and the four second clamping members 62 are set one-to-one with the four second valves 45 of the reagent card box 40.

[0061] As an alternative, the first clamping member 26 of the first clamping assembly 20 and the second clamping member 62 of the second clamping assembly 60 can be centrally mounted on the same mounting plate. This eliminates the need for two mounting plates; a single drive mechanism can be used to move the mounting plate containing the first clamping member 26 and the second clamping member 62. Furthermore, a third or fourth clamping assembly can also be provided, etc., without limitation.

[0062] It should be noted that the first drive mechanism 21 can be, for example, a motor lead screw drive structure, a cylinder drive structure, a hydraulic cylinder drive structure, an electric cylinder drive structure, a cam drive structure, etc., and is not limited here. It can be set according to actual needs. The second drive mechanism 61 is similar to the first drive mechanism 21, and will not be described in detail here.

[0063] Please see Figure 3 , Figure 4 , Figure 11 and Figure 12 , Figure 11 and Figure 12 The diagrams show the slide rail assembly 70 from two different perspectives. In one embodiment, the cartridge valve control assembly further includes the slide rail assembly 70. The slide rail assembly 70 includes a mounting bracket 71 mounted on a support plate 11, a first slide rail 72 and a second slide rail 73 mounted on the mounting bracket 71, a first slider 74 slidably mounted on the first slide rail 72, a second slider 75 slidably mounted on the second slide rail 73, a first connecting plate 76 connected to the first slider 74, and a second connecting plate 77 connected to the second slider 75. The first connecting plate 76 is connected to the first mounting plate 24, and the second connecting plate 77 is connected to the second mounting plate 63. Thus, when the first drive mechanism 21 drives the first mounting plate 24 to move, the first connecting plate 76 drives the first slider 74 to move along the first slide rail 72. This results in better operational stability of the first mounting plate 24, and allows for more precise control of the movement stroke of the first mounting plate 24, i.e., the movement stroke accuracy of the first clamping member 26 reaches 0.05mm. Furthermore, since the first clamping member 26 has high precision in its movement stroke, when the first valve 44 is opened, under the power of the first drive mechanism 21, the first clamping member 26 moves away from the first valve 44 by a distance of, for example, 0.1 mm or 0.2 mm, releasing the first valve 44 of the reagent card box 40. At this time, the first clamping member 26 still abuts against the side of the reagent card box 40 to play a positioning role, so that the reagent card box 40 is stably set on the bracket 10.

[0064] Similarly, when the second drive mechanism 61 drives the second mounting plate 63 to move, the second connecting plate 77 drives the second slider 75 to move along the second slide rail 73. This makes the second mounting plate 63 more stable in operation, and thus the movement stroke of the second mounting plate 63 can be controlled more accurately, that is, the movement stroke accuracy of the second clamping member 62 reaches 0.05mm.

[0065] Specifically, the first mounting plate 24, the second mounting plate 63, the first slider 74, the second slider 75, the first connecting plate 76, and the second connecting plate 77 can be made of materials such as aluminum, copper, iron, stainless steel, wood, plastic, etc. There are no restrictions here, and they can be set according to actual needs.

[0066] It should be noted that the "first connecting plate 76" can be "a part of the first slider 74", that is, the "first connecting plate 76" and "other parts of the first slider 74" are integrally formed; or it can be an independent component that can be separated from "other parts of the first slider 74", that is, the "first connecting plate 76" can be manufactured independently and then combined with "other parts of the first slider 74" to form a whole.

[0067] In one embodiment, the cartridge valve control assembly further includes a first sensor 81 and a first trigger 82 that senses and engages with the first sensor 81. The first sensor 81 is mounted on the bracket 10, and the first trigger 82 is mounted on the first mounting plate 24 or the first connecting plate 76. Thus, the first sensor 81 can sense the movement position of the first trigger 82, and based on the sensing signal from the first trigger 82, the movement position information of the first mounting plate 24 can be obtained, thereby correspondingly controlling the movement stroke of the first clamping member 26 within a preset range.

[0068] In addition, the cartridge valve control assembly also includes a second sensor 83 and a second trigger 84 that senses and cooperates with the second sensor 83. The second sensor 83 is mounted on the support plate 11, and the second trigger 84 is mounted on the second mounting plate 63 or the second connecting plate 77.

[0069] Specifically, the first sensor 81 can be a photoelectric switch, proximity switch, through-beam sensor, or other type of sensor, as long as it can sense the movement of the first trigger 82 and promptly feed back the movement signal of the first trigger 82. The second sensor 83 is similar to the first sensor 81 and will not be described in detail here.

[0070] In one embodiment, the first clamping assembly 20 further includes at least one second elastic element 27 mounted on the first mounting plate 24. At least one first clamping element 26 is correspondingly disposed with at least one second elastic element 27, and the first clamping element 26 is connected to the first mounting plate 24 via the second elastic element 27. Thus, under the buffering effect of the second elastic element 27, damage to the reagent cartridge 40 can be avoided when the first clamping element 26 contacts the first valve 44.

[0071] Similarly, the second clamping assembly 60 also includes at least one third elastic element mounted on the second mounting plate 63, with at least one second clamping element 62 corresponding to at least one third elastic element. The second clamping element 62 is connected to the second mounting plate 63 through the third elastic element. Likewise, under the buffering effect of the third elastic element, the second clamping element 62 can avoid damaging the reagent cartridge 40 during contact with the second valve 45.

[0072] Please see Figure 8 and Figure 9In one embodiment, the first mounting plate 24 has a first receiving chamber 241 and a second guide hole 243 communicating with the first receiving chamber 241. A second elastic member 27 is disposed in the first receiving chamber 241. One end of the first pressing member 26 is located inside the first receiving chamber 241 and is connected to the second elastic member 27. The first pressing member 26 is movably disposed in the second guide hole 243. The other end of the first pressing member 26 extends out of the second guide hole 243 and is used to press or release the first valve 44 of the reagent card box 40.

[0073] Similarly, the second mounting plate 63 has a second receiving chamber (not shown in the figure) and a third guide through hole (not shown in the figure) communicating with the second receiving chamber. The third elastic member is disposed in the second receiving chamber. One end of the second clamping member 62 is located inside the second receiving chamber and is connected to the third elastic member. The second clamping member 62 is movably disposed in the third guide through hole. The other end of the second clamping member 62 extends out of the third guide through hole and is used to clamp or release the second valve 45 of the reagent card box 40.

[0074] Specifically, the first elastic element 25, the second elastic element 27, the third elastic element and the fourth elastic element 53 are all, for example, springs, elastic blocks, etc., and are not limited here. They can be set according to actual needs.

[0075] Please see Figure 13 In one embodiment, the cartridge valve control assembly further includes a pyrolysis assembly 91, mounted on the support plate 11, for heating and pyrolyzing the liquid in the pretreatment chamber 47 of the reagent cartridge 40. Additionally, the cartridge valve control assembly includes a magnetic mixing assembly 92, mounted on the support plate 11, for moving magnetic beads up and down in the mixing chamber 48 of the reagent cartridge 40, thereby ensuring thorough mixing of the liquid in the mixing chamber 48 to obtain the desired mixed liquid.

[0076] Please see Figure 4 In one embodiment, to avoid interference between the first clamping member 26 or the trachea and the guide plate 12 during the process of clamping the reagent card box 40, at least one first through hole 121 and at least one second through hole 122 are provided on the guide plate 12. The at least one first through hole 121 is provided in a one-to-one correspondence with at least one first clamping member 26, allowing the first clamping member 26 to pass through; the at least one second through hole 122 is provided in a corresponding manner with the first connector 221 of the first trachea 22 and also with the third connector 231 of the second trachea 23, allowing the first connector 221 to pass through and the third connector 231 to pass through.

[0077] Please refer to the following: Figures 1 to 9In one embodiment, an in vitro analytical diagnostic testing device includes a cartridge valve control component according to any of the above embodiments.

[0078] When the above-mentioned in vitro analytical diagnostic testing device needs to test the sample liquid in the reagent cartridge 40, the reagent cartridge 40 is inserted into the support 10. The first driving mechanism 21 moves the first air tube 22 and the second air tube 23 to a first position, so that the first connector 221 is connected to the first air port 41 and the third connector 231 is connected to the second air port 42. When the gas path control component 31 is working in the first working state, the first air port 41 is connected to the pressure control mechanism 32 through the first air tube 22, and the second air port 42 is connected to the first air tube 22 through the second air tube 231. The second vent 23 is connected to the atmospheric environment, and the suction force provided by the pressure control mechanism 32 acts on the reagent cartridge 40, causing the sample liquid inside the reagent cartridge 40 to transfer between chambers. Similarly, when the gas path control component 31 is working in the second working state, the second vent 42 is connected to the pressure control mechanism 32 through the second vent 23, and the first vent 41 is connected to the atmospheric environment through the first vent 22. The suction force provided by the pressure control mechanism 32 acts on the reagent cartridge 40, causing the sample liquid inside the reagent cartridge 40 to transfer between chambers. In this way, by switching the gas path control component 31, the first vent 41 of the reagent cartridge 40 can be connected to the pressure control mechanism 32 through the first vent 22, and the second vent 42 of the reagent cartridge 40 can be connected to the pressure control mechanism 32 through the second vent 23. This provides power to drive the sample liquid to transfer between chambers according to the process requirements of the reagent cartridge 40, while simplifying the overall structure of the cartridge valve control component.

[0079] In one specific embodiment, the operation of the cartridge valve control assembly includes the following steps:

[0080] Step S10: Sample addition. The patient sample is added to the sample chamber 46 of the reagent cartridge 40. The lid of the reagent cartridge 40 is closed, and the reagent cartridge 40 is simultaneously inserted into the support 10. The limiting baffle 50 acts as a limit to prevent the reagent cartridge 40 from falling off the support 10.

[0081] Step S20: After the reagent card box 40 is installed on the bracket 10, the first clamping assembly 20 and the second clamping assembly 60 work. The first driving mechanism 21 drives the first mounting plate 24 to move closer to the bracket 10, and the second driving mechanism 61 drives the second mounting plate 63 to move closer to the bracket 10, so that the first connector 221 is connected to the first air hole 41 and the third connector 231 is connected to the second air hole 42. At the same time, the three first clamping parts 26 are in close contact with the three first valves 44 of the reagent card box 40, and the other first clamping part 26 is in contact with the reagent card box 40. The four second clamping parts 62 are in close contact with the four second valves 45 of the reagent card box 40, so that the reagent card box 40 is clamped and fixed.

[0082] Step S30: After the reagent cartridge 40 is fully clamped, the control gas path control component 31 is put into the first working state, so that the second vent 42 is connected to the atmospheric environment and the first vent 41 is connected to the plunger pump. The plunger pump provides a negative pressure to the first vent 41 through the reverse movement of the piston, so that the sample liquid in the reagent cartridge 40 moves from the sample chamber to the pretreatment chamber 47.

[0083] In step S40, after the sample liquid is transferred and fills the pretreatment chamber 47, the first drive mechanism 21 is controlled to move the first mounting plate 24, causing the first mounting plate 24 to move the first clamping member 26 away from the reagent cartridge 40 by a preset distance, so that the three first valves 44 of the reagent cartridge 40 can be opened. At the same time, the gas path control component 31 is controlled to work in the second working state, so that the first vent 41 is connected to the atmospheric environment, and the second vent 42 is connected to the plunger pump. The plunger pump provides a negative pressure to the second vent 42 through the reverse movement of the piston. At this time, the lysed sample liquid is drawn into the two mixing chambers 48 to rehydrate the lyophilized reagents therein.

[0084] Step S50: Drive the first driving mechanism 21 to move a preset distance so that the three first clamping parts 26 are in close contact with the three first valves 44 of the reagent card box 40, that is, close the three first valves 44 and turn on the magnetic mixing assembly 92. By periodically switching the power on and off the magnetic mixing assembly 92, it generates periodic magnetic force, which drives the magnetic beads arranged in the mixing chamber 48 to move up and down reciprocally, so as to mix the reconstituted lyophilized reagent and sample liquid evenly.

[0085] Step S60: After mixing, the second driving mechanism 61 is moved a preset distance to separate the four second clamping parts 62 from the four second valves 45, thus opening the four second valves 45. The forward movement of the piston in the plunger pump pressurizes the second vent 42, allowing the sample to flow from the mixing chamber 48 into the two PCR chambers 49.

[0086] Step S70: While maintaining a certain pressure, the four second clamping members 62 tightly abut against the four second valves 45, closing the four second valves 45 and causing the PCR chamber of the reagent cartridge 40 to bulge slightly. Simultaneously with the closure of the four second valves 45, the optical window corresponding to the PCR chamber 49 also presses firmly against the front of the PCR chamber 49 of the reagent cartridge 40. Due to the pressure on the PCR chamber 49 of the reagent cartridge 40, it bulges slightly, allowing it to adhere tightly to the PCR optical window, thus facilitating the next step.

[0087] In step S80, the first drive mechanism 21 and the second drive mechanism 61 reset, the first clamping member 26, the second clamping member 62, the first connector 221 and the third connector 231 all loosen the reagent card box 40, and the plunger pump resets.

[0088] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0089] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

[0090] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0091] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0092] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0093] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0094] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

Claims

1. A cartridge valve control assembly, characterized in that, The cartridge valve control assembly includes: The support is used to hold the reagent card holder; The first clamping assembly includes a first driving mechanism, a first air tube, and a second air tube. The first driving mechanism is mounted on the bracket and drives the first air tube and the second air tube to move. One end of the first air tube is provided with a first connector, and the other end of the first air tube is provided with a second connector. One end of the second air tube is provided with a third connector, and the other end of the second air tube is provided with a fourth connector. When the first driving mechanism drives the first air tube and the second air tube to a first position, the first connector and the third connector are respectively used to connect with the first air hole and the second air hole of the reagent card box. The gas path control component and the pressure control mechanism are respectively connected to the second connector, the fourth connector, and the pressure control mechanism. The gas path control component has a first working state and a second working state. When the gas path control component operates in the first working state, it controls the second connector to communicate with the pressure control mechanism, and the fourth connector to communicate with the atmospheric environment. When the gas path control component operates in the second working state, it controls the second connector to communicate with the atmospheric environment, and the fourth connector to communicate with the pressure control mechanism. The first clamping assembly further includes a first mounting plate; the first air pipe and the second air pipe are both disposed on the first mounting plate; the first driving mechanism is connected to the first mounting plate, and the first driving mechanism drives the first mounting plate to move; the first mounting plate is provided with a first guide through hole, and the first air pipe is movably disposed in the first guide through hole.

2. The cartridge valve control assembly according to claim 1, characterized in that, Both the first connector and the third connector are vacuum suction cups.

3. The cartridge valve control assembly according to claim 1, characterized in that, The first clamping assembly further includes a first elastic element; the first air tube is connected to the first mounting plate through the first elastic element.

4. The cartridge valve control assembly according to claim 3, characterized in that, A first step is provided on the wall of the first guide hole, and a second step is provided on the outer wall of the first air tube. The two ends of the first elastic member respectively abut against the first step and the second step.

5. The cartridge valve control assembly according to claim 1, characterized in that, The first clamping assembly further includes at least one first clamping member; at least one first clamping member is mounted on the first mounting plate; the first clamping member is used to clamp or release the first valve of the reagent card box.

6. The cartridge valve control assembly according to claim 5, characterized in that, The cartridge valve control assembly further includes a second clamping assembly; the second clamping assembly includes a second drive mechanism, a second mounting plate and at least one second clamping member; the second drive mechanism is disposed on the bracket, and at least one second clamping member is mounted on the second mounting plate; the second drive mechanism is connected to the second mounting plate and is used to drive the second mounting plate to move closer to or away from the bracket.

7. The cartridge valve control assembly according to claim 6, characterized in that, The cartridge valve control assembly further includes a slide rail assembly; the slide rail assembly includes a fixed frame mounted on the bracket, a first slide rail and a second slide rail disposed on the fixed frame, a first slider slidably disposed on the first slide rail, a second slider slidably disposed on the second slide rail, a first connecting plate connected to the first slider, and a second connecting plate connected to the second slider; the first connecting plate is connected to the first mounting plate, and the second connecting plate is connected to the second mounting plate.

8. The cartridge valve control assembly according to claim 7, characterized in that, The cartridge valve control assembly further includes a first sensor and a first trigger that cooperates with the first sensor; the first sensor is mounted on the bracket, and the first trigger is mounted on the first mounting plate or the first connecting plate; the cartridge valve control assembly further includes a second sensor and a second trigger that cooperates with the second sensor; the second sensor is mounted on the bracket, and the second trigger is mounted on the second mounting plate or the second connecting plate.

9. The cartridge valve control assembly according to claim 6, characterized in that, The first clamping assembly further includes at least one second elastic element mounted on the first mounting plate, wherein at least one first clamping element and at least one second elastic element are respectively provided, and the first clamping element is connected to the first mounting plate through the second elastic element; The second clamping assembly further includes at least one third elastic element mounted on the second mounting plate. At least one second clamping element and at least one third elastic element are respectively arranged in a one-to-one correspondence. The second clamping element is connected to the second mounting plate through the third elastic element.

10. The cartridge valve control assembly according to claim 9, characterized in that, The first mounting plate has a first receiving chamber and a second guide hole communicating with the first receiving chamber. The second elastic member is disposed in the first receiving chamber. One end of the first clamping member is located inside the first receiving chamber and is connected to the second elastic member. The first clamping member is movably disposed in the second guide hole. The other end of the first clamping member extends out of the second guide hole and is used to clamp or loosen the first valve of the reagent card box. The second mounting plate has a second receiving chamber and a third guide hole communicating with the second receiving chamber. The third elastic member is located in the second receiving chamber. One end of the second clamping member is located inside the second receiving chamber and is connected to the third elastic member. The second clamping member is movably disposed in the third guide hole. The other end of the second clamping member extends out of the third guide hole and is used to clamp or release the second valve of the reagent card box.

11. An in vitro analytical diagnostic detection device, characterized in that, The in vitro analysis and diagnostic testing device includes the cartridge valve control assembly as described in any one of claims 1 to 10.

Images