A fungicide composition containing clofoconazole and quinoline copper and its application

Abstract

The present invention discloses a pesticide composition containing quinoline copper and clofoconazole and its application. The pesticide composition comprises active ingredients clofoconazole and active ingredient quinoline copper, and the mass ratio of the two is 1:50 to 50:1. The present invention can effectively prevent and treat diseases such as potato, tomato early blight, cucumber target spot and the like. The present application compounds clofoconazole and quinoline copper, and the two can produce a synergistic effect, thereby making the formed pesticide fungicide have a good fungicide effect, and has good activity against diseases such as potato, tomato early blight, cucumber target spot and the like; and the mechanisms of action of the active ingredients in the fungicide composition are different from each other, so that the sites of action are increased, the control spectrum is expanded, and the single selection pressure on the pathogen is reduced, which is conducive to delaying the speed of the generation of disease resistance, and the dosage is low and the cost is low. At the same time, the fungicide composition has low side effects and will not affect the safety and yield of crops.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a pesticide fungicide composition containing clofoconazole and quinoline copper for preventing and controlling diseases such as potato and tomato early blight and cucumber target spot disease, and application thereof. Background Art

[0002] Potato early blight pathogen ( Alternaria solani ) usually overwinters in the form of pathogen spores in diseased plant debris, diseased seed potatoes or diseased soil. The pathogen spreads with the help of wind and rain, and invades the potato leaves through the lenticels, stomata or injured parts the following year, producing conidia, causing the plants to become sick; potato early blight can harm the leaves, petioles, stems and tubers of the plant. Generally, when attacked by early blight, the lower old leaves of the potato are infected first, and around the potato-setting period, the disease can also harm the new young leaves, causing a large number of leaves to wither prematurely or even die, thereby seriously damaging potato yields; in recent years, early blight can be seen in most potato-growing areas in China, and the degree of damage has gradually increased, causing a large-scale decline in my country's potato yields, and greatly reducing the quality and quality of potatoes, which has had a great impact on my country's potato production.

[0003] Cucumber target spot is caused by polyhomocybe spores of the subdivision Ascomycota ( Corynespora cassiicola (Berk & Curt) Wei) was first reported in my country in the 1960s. Due to its rarity, it has not received enough attention. In recent years, with the continuous expansion of the scale of cucumber facility cultivation, the occurrence of this disease has become increasingly serious and has become one of the main diseases in cucumber production; tomato early blight ( Alternaria solani ), also known as ring rot, is one of the important diseases of tomatoes. The disease occurs throughout the country. It usually occurs during the flowering period or early fruiting period of tomatoes, harming the leaves, petioles, stems and fruits of tomatoes, causing leaf and flower fall, fruit fall, rotten fruit and broken branches, which has a serious impact on tomato growth.

[0004] At present, diseases such as potato early blight, cucumber target spot, and tomato early blight are mainly controlled by chemical agents. However, the large-scale use of chemical agents can easily cause pesticide residues, causing harm to human health. At the same time, long-term application of a single chemical pesticide can easily lead to pesticide resistance.

[0005] In view of the severity of the three diseases and the harmfulness of chemical pesticides, the present invention, based on this demand, proposes to utilize the different mechanisms of action of the active ingredients in the combined compounds, increase the action sites, expand the prevention and control spectrum and reduce the single selection pressure on the pathogens, which is beneficial to delay the speed of the development of disease resistance, and has low dosage and low cost. At the same time, the fungicide composition has low side effects and will not affect the safety and yield of crops.

[0006] Mefentrifluconazole is the first triazole fungicide containing an isopropanol structure. Its chemical name is (2RS)-2-[4-(4-chlorophenoxy)-α,α,α-trifluoro-o-tolyl]-1-(1H-1,2,4-triazol-1-yl)propan-2-ol, CAS number is 1417782-03-6, and its molecular formula is C 18 H 15 ClF3N3O2, relative molecular mass is 397.8, the molecular structure of clofoconazole is shown in Formula 1. Clofoconazole is soluble in water and organic solvents. At 20°C, its solubility in water is 0.81 mg / L. It has low water solubility and volatility and will not enter groundwater through leaching. Its solubility in organic solvents acetone, ethyl acetate, xylene and 1,2-dichloroethane is 93.2, 116.2, 8.5 and 55.3 mg / L respectively. The melting point of clofoconazole is 126°C, and the degradation point is 300°C. It will decompose in a boiling state, but it is not easy to burn.

[0007]

[0008] Cyfluthrin is a new type of isopropanol triazole fungicide with broad spectrum, high efficiency, systemic, eradication and protection effects, belonging to the class of sterol demethylation inhibitors. Unlike the widely used triazole fungicides, Cyfluthrin contains an isopropanol structure, which can reduce pathogen mutation and delay pathogen resistance.

[0009] Quinoline copper is a broad-spectrum, highly effective, low-toxic chelated organic copper fungicide that has excellent preventive and therapeutic effects on fungal and bacterial diseases of crops. It has the dual fungicidal effects of organic quinoline and copper preparations, has multiple target sites, and is not easy to produce resistance after repeated use. It is highly effective against conventional fungicide-resistant strains and has a long duration of efficacy. In current production, quinoline copper is mainly used to prevent and control bacterial or fungal diseases such as tomato early blight, bacterial canker, pepper blight, canker, scab, downy mildew, cucumber downy mildew, cucumber target spot, bacterial leaf spot, litchi downy mildew, watermelon downy mildew, anthracnose, bacterial fruit spot, potato early blight, etc.

[0010] Since the prevention and control of potato, tomato early blight, and cucumber target spot is difficult and harmful, the commonly used control agents such as prochloraz are old agents with a long history and high resistance, and the control effect is not good. In public reports, there is no in-depth study on the activity of clofoconazole and quinoline copper on potato, tomato early blight, and cucumber target spot, and there is no report on the compounding of clofoconazole and quinoline copper. Therefore, the prior art combines different agents and then prevents and controls the disease, but the effect of the combination of different agents is uncertain, which may produce a synergistic effect or an antagonistic effect. And the effect of the combination of different agents on different diseases is also uncertain. Summary of the invention

[0011] In order to solve the technical problems existing in the background technology, the present application proposes a pesticide fungicide containing clofodimazole and quinoline copper for preventing and controlling diseases such as potato and tomato early blight and cucumber target spot.

[0012] The objective of the present invention is achieved through the following technical solutions:

[0013] A pesticide fungicide containing cypermethrin and quinoline copper, wherein the active ingredients of the pesticide fungicide are cypermethrin and quinoline copper, and the pesticide fungicide is characterized in that the mass ratio of cypermethrin to quinoline copper is 1-50:50-1.

[0014] The mass of clofoconazole accounts for 1-40% of the mass of the pesticide fungicide; the mass of quinoline copper accounts for 1-45% of the mass of the pesticide fungicide.

[0015] As a preferred technical solution, it is characterized in that: the mass of the clofoconazole accounts for 10% of the mass of the pesticide fungicide; the mass of the quinoline copper accounts for 30% of the mass of the pesticide fungicide.

[0016] As a preferred technical solution, it is characterized in that: the bactericidal composition also includes the following substances by mass percentage: 1-9% dispersant, 3-5% wetting agent, 0.1-2.5% thickener, 0.1-4% preservative, 1-8% antifreeze agent, and the balance is water.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In the public reports, the activities of clofoconazole and quinoline copper on diseases such as potato and tomato early blight and cucumber target spot have not been studied in depth, and the compounding of clofoconazole and quinoline copper has not been reported. The compounding of the fungicide composition containing clofoconazole and quinoline copper of the present invention has obvious synergistic effect, can effectively improve the control effect on diseases such as potato and tomato early blight and cucumber target spot, can reduce the dosage of active ingredients, reduce production costs, and reduce environmental pollution. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0020] The embodiment of the present invention provides a pesticide fungicide, wherein the raw materials of the active ingredients of the pesticide fungicide are composed of cypermethrin and quinoline copper. The embodiment of the present invention specifically selects cypermethrin and quinoline copper for compounding, and the two can produce a synergistic effect, thereby making the formed pesticide fungicide have a good bactericidal effect and a large prevention and control spectrum, especially having a good prevention and control effect on potato early blight and cucumber target spot.

[0021] Ingredients in the embodiments of the present invention:

[0022] The wetting and dispersing agent can be selected from one or two of TERSPERSE 4894, TERSPERSE 2500, phosphate anionic surfactant, polyoxyethylene nonionic surfactant, alkylphenol polyoxyethylene phosphate, fatty acid polyoxyethylene ether, alkylnaphthalene sulfonate mixture, polycarboxylate, alkylnaphthalene sulfonate mixture, sodium methylene bisnaphthalene sulfonate, sodium lauryl sulfate, potassium phosphate, calcium lignin sulfonate, ammonium sulfate, polysulfate, polycarboxylate, calcium dodecylbenzene sulfonate, pull-open powder BX, alkylphenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, and TERSPERSE 2280;

[0023] The thickener may be selected from any one or more of xanthan gum, carboxymethyl cellulose, sodium polyacrylate, magnesium aluminum silicate, fumed silica or attapulgite.

[0024] The antifreeze agent can be selected from any one of ethylene glycol, propylene glycol, glycerol, glycerol-ether diglycol or methyl propylene diglycol, or a mixture of more than one of them in any proportion;

[0025] The stabilizer may be selected from any one or more of 2,6-di-tert-butyl-4-methylphenol (BHT), citric acid or butylated hydroxyanisole (BHA).

[0026] The preservative may be sodium benzoate.

[0027] It should be noted that the fungicide provided by the present invention is used to prevent and treat diseases of crops including but not limited to potato, tomato early blight, and cucumber target spot disease, but the pesticide fungicide has a better control effect on potato, tomato early blight, and cucumber target spot disease. In addition, the pesticide fungicide will not affect the normal growth of plants. The pesticide fungicide is a liquid preparation, preferably a suspension. It should be noted that the embodiments of the present invention only list suspensions, but it is understandable that other types of liquid preparations are also within the scope of protection of the embodiments of the present invention.

[0028] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0029] Example 1

[0030] The embodiment of the present invention provides a bactericidal composition, wherein the active ingredients of the bactericidal composition are cypermethrin and quinoline copper, and the mass ratio of cypermethrin to quinoline copper is 10:5.

[0031] Example 2

[0032] The embodiment of the present invention provides a bactericidal composition, wherein the active ingredients of the bactericidal composition are cypermethrin and quinoline copper, and the mass ratio of cypermethrin to quinoline copper is 10:10.

[0033] Example 3

[0034] The embodiment of the present invention provides a bactericidal composition, wherein the active ingredients of the bactericidal composition are cypermethrin and quinoline copper, and the mass ratio of cypermethrin to quinoline copper is 10:15.

[0035] Example 4

[0036] The embodiment of the present invention provides a bactericidal composition, wherein the active ingredients of the bactericidal composition are cypermethrin and quinoline copper, and the mass ratio of cypermethrin to quinoline copper is 10:20.

[0037] Example 5

[0038] The embodiment of the present invention provides a bactericidal composition, wherein the active ingredients of the bactericidal composition are cypermethrin and quinoline copper, and the mass ratio of cypermethrin to quinoline copper is 10:30.

[0039] Example 6

[0040] This embodiment provides a bactericidal composition, the components of which are as follows in Table 1 by mass percentage:

[0041] Table 1 The dosage ratio of each component in the bactericidal composition

[0042] Ingredient name content(%) Cyclohexanil 10 Copper quinoline 5 Alkylphenol polyoxyethylene phosphate 3 Fatty acid polyoxyethylene ether 1 Xanthan gum 0.2 Magnesium aluminum silicate 1.6 Sodium Benzoate 0.5 Ethylene glycol 4 Silicone defoamer 0.2 water Make up the margin

[0043] Example 7

[0044] This embodiment provides a bactericidal composition, the components of which are as shown in Table 2 by mass percentage:

[0045] Table 2 The dosage ratio of each component in the bactericidal composition

[0046] Ingredient name content(%) Cyclohexanil 10 Copper quinoline 10 Alkylphenol polyoxyethylene phosphate 4 Fatty acid polyoxyethylene ether 2 Xanthan gum 0.15 Magnesium aluminum silicate 2 Sodium Benzoate 0.5 Ethylene glycol 4 Silicone defoamer 0.2 water Make up the margin

[0047] Example 8

[0048] This embodiment provides a bactericidal composition, the components of which are as shown in Table 3 by mass percentage:

[0049] Table 3 The dosage ratio of each component in the bactericidal composition

[0050] Ingredient name content(%) Cyclohexanil 10 Copper quinoline 15 Alkylphenol polyoxyethylene phosphate 4 Fatty acid polyoxyethylene ether 2.5 Xanthan gum 0.2 Magnesium aluminum silicate 1.7 Sodium Benzoate 0.6 Ethylene glycol 4 Silicone defoamer 0.2 water Make up the margin

[0051] Example 9

[0052] This embodiment provides a bactericidal composition, the components of which are as shown in Table 4 by mass percentage:

[0053] Table 4 The dosage ratio of each component in the bactericidal composition

[0054] Ingredient name content(%) Cyclohexanil 10 Copper quinoline 20 Alkylphenol polyoxyethylene phosphate 4.5 Fatty acid polyoxyethylene ether 3 Xanthan gum 0.2 Magnesium aluminum silicate 1.2 Sodium Benzoate 0.5 Ethylene glycol 4.5 Silicone defoamer 0.2 water Make up the margin

[0055] Example 10

[0056] This embodiment provides a bactericidal composition, the components of which are as shown in Table 5 by mass percentage:

[0057] Table 5 The dosage ratio of each component in the bactericidal composition

[0058] Ingredient name content(%) Cyclohexanil 10 Copper quinoline 30 Alkylphenol polyoxyethylene phosphate 5 Fatty acid polyoxyethylene ether 4 Xanthan gum 0.15 Magnesium aluminum silicate 0.5 Sodium Benzoate 0.6 Ethylene glycol 4.5 Silicone defoamer 0.2 water Make up the margin

[0059] The invention provides a method for preparing the pesticide composition into a pesticide suspension, comprising: mixing quinoline copper and clofoconazole with a wetting agent, a dispersant, an antifreeze agent, a preservative, a part of a thickener (50-70% of the total mass), a part of a defoamer (50-70% of the total mass) and water, and grinding them uniformly; stopping the grinding when the particle size is 4 μm; and adding the remaining thickener and defoamer to adjust uniformly to obtain a suspension.

[0060] Experimental Example 1 Biological Activity Test

[0061] According to the guidelines for indoor bioassay of pesticides NY / T 1156.2-2006 and NY / T1156.6-2006, the effects of clofoconazole, quinoline copper and their mixed ratios on potato early blight were determined to screen the optimal mixing ratio of the two fungicides.

[0062] A. Potato early blight pathogen ( Alternaria solani ) was provided by the Plant Pathology Laboratory of Qingdao Agricultural University.

[0063] B. The test agents are 97% clofoconazole technical (purchased from BASF Europe) and 98% quinoline copper technical (purchased from Xingnong Co., Ltd.)

[0064] C. The mixing ratio of clofothiazide: quinoline copper is: 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4. The concentrations are shown in Table 6:

[0065] Table 6 The dosage ratio of each substance in the bactericidal composition

[0066]

[0067] D. Toxicity test method

[0068] The preserved potato early blight bacteria were activated and cultured in the dark at 25℃ for 5 days. Mycelium blocks with a diameter of 5 mm were punched out from the edge of the cultured colony with a sterilized puncher and transferred to the drug-containing flat plate culture medium respectively. Three replicates were set for each treatment. Cultured in a constant temperature incubator at 25℃, when the diameter of the control colony grew to about 2 / 3 of the diameter of the culture dish, the colony diameter was measured by the cross method, and the average colony diameter and mycelium growth inhibition rate were calculated. The average inhibition rate of the three replicates was converted into a probability value, and the concentration of the drug was converted into a logarithmic value. Then, the toxicity index of each drug and the co-toxicity coefficient of the mixture were calculated according to the Sun Yunpei method.

[0069]

[0070] DPS statistical software was used to perform regression analysis on the logarithmic values ​​of treatment concentrations of each single agent and different ratios of mixed combinations and the corresponding inhibition rate probability values, and the toxicity regression curve and EC were calculated. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to the Sun Yunpei method.

[0071] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (1), formula (2) and formula (3):

[0072]

[0073] Where: ATI —Measured toxicity index of the mixture;

[0074] S —EC of standard fungicides 50 , the unit is milligrams per liter (mg / L);

[0075] M —EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0076]

[0077] Where: TTI —Theoretical toxicity index of mixture;

[0078] A —A. Toxicity index of the agent;

[0079] P A —The percentage of agent A in the mixture, in percentage (%);

[0080] B —B Agent toxicity index;

[0081] P B —The percentage of agent B in the mixture, in percentage (%).

[0082]

[0083] In the formula: CTC is the co-toxicity coefficient; ATI is the measured toxicity index of the mixture; TTI is the theoretical toxicity index of the mixture.

[0084] The co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; 80<CTC<120 indicates an additive effect.

[0085] E. Test results Tables 7 and 8:

[0086] Table 7 Toxicity test results of chlorfenapyr, quinoline copper and 7 ratios

[0087]

[0088] Table 8 The results of the combined toxicity test of clofoconazole and quinoline copper on potato early blight

[0089]

[0090] The study showed (Table 8) that the effective inhibitory concentrations of 7 mixtures (clofoxyclobutanil: quinoline copper = 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4) on potato early blight were 0.7838, 0.8534, 0.9618, 1.2368, 1.5792, 1.9501 and 2.4948 mg / L, respectively, and the co-toxicity coefficients (CTC) were 133.28, respectively.

[0091] , 129.34 , 126.71 , 124.45 , 132.25, 130.35 , 117.15 It can be seen that the ratios of clofothiazolin and quinoline copper 4:1, 3:1, 2:1, 1:1, 1:2, and 1:3 all showed synergistic effects, and the ratio of 1:4 showed an additive effect.

[0092] Experimental Example 2 Field efficacy test

[0093] Field efficacy test 1: The fungicidal composition prepared in Example 10 was used to control potato early blight in the field.

[0094] 1. Test methods

[0095] The test agents are shown in Table 9 below:

[0096] Table 9 Dosage table of each drug test

[0097]

[0098] Guojiazhuang Village, Jimo District, Qingdao City (36.433217 N, 120.4822 E). The experimental field area is 2 mu, the soil pH is 6.4, and the organic matter content is 1.8%. The experimental crop is potato, Kexin No. 1, with a row spacing of 60×20 cm. Potato early blight has occurred in the past three years, and pesticides are used every year. The soil type is loess soil with a neutral pH value. 1000 kg of farmyard manure, 40 kg of urea, 25 kg of ordinary superphosphate, and 30 kg of potassium sulfate are applied per mu. Watering is once, and the management level is average. Potatoes are sown in summer. All experimental plots have the same cultivar and management conditions, and the experimental target is potato early blight. The application method is conventional spraying. The first application of pesticides is carried out at the early stage of potato early blight (July 5), and the second application is carried out on July 12 after an interval of 7 days. This experiment uses pesticides twice in total.

[0099] A commonly used pressure storage manual sprayer (commercially available) was used for liquid spraying, with an adjustable conical nozzle, a pressure of 0.3 MPa, a spray speed of 0.6 liters / minute, and a liquid dosage of 600 liters per hectare. The incidence of potato early blight was investigated on site in the field before the first application of pesticides. The investigation was conducted 7 days after the last application of pesticides. Samples were taken at 5 points in each plot, 3 plants were investigated at each point, and all leaves of each plant were investigated. The disease level was divided according to the percentage of the lesion area on each leaf to the entire leaf area:

[0100] The grading standards are as follows:

[0101] Level 0: no lesions;

[0102] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0103] Level 3: The lesion area accounts for 6%-10% of the entire leaf area;

[0104] Level 5: The lesion area accounts for 11%-20% of the entire leaf area;

[0105] Level 7: The lesion area accounts for 21%-50% of the entire leaf area;

[0106] Level 9: The lesion area accounts for more than 51% of the entire leaf area;

[0107]

[0108] 2. Test results

[0109] The control effects of different pesticides on potato early blight are shown in Table 10 below.

[0110] Table 10 The control effect of different pesticides on potato early blight

[0111]

[0112] According to the above table, the results of the control effect investigation show that Example 10, the preparation dosage of 60 ml / mu has the best control effect, with a control effect of 85.80%, which is significantly better than the control effects of the two single-dose control agents. Through field observation, the above test agents and control agents are safe for the test crop potato, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity, etc.) were found.

[0113] Field efficacy test 2: The fungicide composition prepared in Example 10 was used to control cucumber target spot disease in the field.

[0114] 1. Test methods

[0115] The test agents are shown in Table 11 below:

[0116] Table 11 Dosage table of each drug test

[0117]

[0118] Guojiazhuang Village, Jimo District, Qingdao City (36.435456 north latitude, 120.5324 east longitude, the experimental field area is 2 mu, the soil pH value is 6.4, and the organic matter content is 1.8%. The experimental crop is cucumber, Youliang No. 2, with a plant spacing of 30 cm and a row spacing of 80 cm. The field management is uniform and consistent, which is in line with local agricultural production practices. It was transplanted on April 15, 2021. Cultivation management is carried out in accordance with local scientific cultivation measures. The test target is cucumber target spot disease. The application method is conventional spraying. The first application of the drug was carried out at the early stage of cucumber target spot disease (May 28), 7 days after the second application on June 4, and the drug was used twice in this experiment.

[0119] A commonly used pressure storage manual sprayer (commercially available) was used for liquid spraying, with an adjustable conical nozzle, a pressure of 0.3 MPa, a spray speed of 0.6 liters / minute, and a liquid dosage of 750 liters per hectare. The incidence of cucumber target spot disease was investigated on the field before the first application of pesticides. The investigation was conducted 7 days after the last application of pesticides. Five points were randomly selected for investigation in each plot, and five plants were investigated at each point. All leaves of each plant were investigated. The total number of leaves investigated and the number of diseased leaves at each level were recorded. The disease level was divided according to the percentage of the diseased spot area on each leaf to the entire leaf area:

[0120] The grading standards are as follows:

[0121] Level 0: no lesions;

[0122] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0123] Level 3: The lesion area accounts for 6%-10% of the entire leaf area;

[0124] Level 5: The lesion area accounts for 11%-20% of the entire leaf area;

[0125] Level 7: The lesion area accounts for 21%-40% of the entire leaf area;

[0126] Level 9: The lesion area accounts for more than 40% of the entire leaf area;

[0127]

[0128] 2. Test results

[0129] The control effects of different pesticides on cucumber target spot disease are shown in Table 12 below.

[0130] Table 12 The control effect of different pesticides on cucumber target spot disease

[0131]

[0132] According to the above table, the results of the control effect investigation show that in Example 10, the dosage of the preparation is 60mL / mu, and the control effect is the best, with a control effect of 87.12%, which is significantly better than the control effect of the two single-dose control agents. According to field observations, the above-mentioned test agents and control agents are safe for the test crop cucumber, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity, etc.) were found.

[0133] Field efficacy test three: The fungicide composition prepared in Example 10 was used to control tomato early blight in the field.

[0134] 1. Test methods

[0135] The test agents are shown in Table 13 below

[0136] Table 13 Dosage table of each drug test

[0137]

[0138] Guojiazhuang Village, Jimo District, Qingdao City (36.467832N, 120.4412E, the experimental field area is 2 mu, the soil pH is 6.4, and the organic matter content is 1.8%. The experimental crop is tomato, base powder king, seedlings were raised on June 11, 2021, land was prepared on July 3, 2021, 30kg of compound fertilizer (15-15-15) was used as base fertilizer per mu, and artificial transplanting was carried out on July 5, 2021. There are about 3000 plants per mu, with a plant spacing of 30cm and a row spacing of 50cm.

[0139] The cultivation and management conditions were in line with local agricultural production practices. The test target was tomato early blight. The pesticide application method was conventional spraying. The first application was carried out at the early stage of tomato early blight (August 10), and the second application was carried out 7 days later on August 17. The total application of the pesticide was 2 times in this experiment.

[0140] A commonly used pressure storage manual sprayer (commercially available) was used for liquid spraying, with an adjustable conical nozzle, a pressure of 0.3 MPa, a spray speed of 0.6 liters / minute, and a liquid dosage of 600 liters per hectare. The incidence of tomato early blight was investigated on site in the field before the first application of pesticides. The investigation was conducted 7 days after the last application of pesticides. Five points were randomly selected for investigation in each plot, with two plants investigated at each point and 10 leaves investigated for each plant. The total number of leaves investigated and the number of diseased leaves at each level were recorded, and the disease level was divided according to the percentage of the lesion area on each leaf to the total leaf area:

[0141] The grading standards are as follows:

[0142] Level 0: no lesions;

[0143] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0144] Level 3: The lesion area accounts for 6%-10% of the entire leaf area;

[0145] Level 5: The lesion area accounts for 11%-20% of the entire leaf area;

[0146] Level 7: The lesion area accounts for 21%-50% of the entire leaf area;

[0147] Level 9: The lesion area accounts for more than 51% of the entire leaf area;

[0148]

[0149] 2. Test results

[0150] The control effects of different pesticide treatments on tomato early blight are shown in Table 14 below.

[0151] Table 14 The control effect of different pesticides on tomato early blight

[0152]

[0153] According to the above table, the results of the control effect investigation show that Example 10, the preparation dosage of 60mL / mu has the best control effect, with a control effect of 87.41%, which is significantly better than the control effects of the two single-dose control agents. Through field observation, the above test agents and control agents are safe for the test crop tomatoes, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity, etc.) were found.

[0154] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A pesticide fungicide containing clofoconazole and quinoline copper, characterized in that: The active ingredients are fluazifop and quinoline copper, and the mass ratio of fluazifop and quinoline copper is 10:(5-30).

2. The pesticide fungicide containing clofoconazole and quinoline copper according to claim 1, characterized in that: The mass of clofoconazole accounts for 10% of the mass of the pesticide fungicide; the mass of quinoline copper accounts for 5-30% of the mass of the pesticide fungicide.

3. The pesticide fungicide containing clofoconazole and quinoline copper according to claim 1, characterized in that: The mass of the clofoconazole accounts for 10% of the mass of the pesticide fungicide; the mass of the quinoline copper accounts for 30% of the mass of the pesticide fungicide.

4. The pesticide fungicide containing cypermethrin and quinoline copper according to claim 1, characterized in that: The pesticide fungicide is used to prevent and control potato early blight, tomato early blight and cucumber target spot diseases.

5. The pesticide fungicide containing cypermethrin and quinoline copper according to any one of claims 1 to 4, characterized in that: The pesticide bactericide is in the form of a liquid preparation.

6. The pesticide fungicide containing clofodinate and quinoline copper according to claim 5, characterized in that: The pesticide fungicide further comprises the following substances by mass percentage: 1-4% dispersant, 3-5% wetting agent, 0.65-1.4% thickener, 0.5-8% preservative, 4-4.5% antifreeze agent, and the balance is water.

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