Hypochlorous acid disinfectant, preparation method and application thereof

Abstract

The application provides a hypochlorous acid disinfectant, a preparation method and application thereof. The application adopts a special process, generates a hypochlorous acid aqueous solution by electrolyzing 0.5-5% NaCl aqueous solution, and utilizes the synergistic effect of hypochlorous acid and hydroxyl radicals in the hypochlorous acid aqueous solution prepared by the application to directly break the cell membrane and degrade DNA and RNA to realize flash sterilization and broad-spectrum sterilization. In the hypochlorous acid disinfectant of the application, hydroxyl and H+ can form a specific mode of binary complex structure and stably coexist for a long time to overcome the problem of extreme instability of traditional hypochlorous acid disinfectant. The disinfecting factor in the disinfectant of the application is efficient, safe and stable, has no irritation to skin mucosa and is friendly to environment. After environment disinfection by using the disinfectant of the application, virus nucleic acid, DNA and RNA can be degraded, which is an international innovation, can fill the gap in domestic and foreign markets and greatly reduces the probability of virus transmission.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hypochlorous acid, in particular to a hypochlorous acid disinfectant, a preparation method and application thereof. BACKGROUND

[0002] The hypochlorous acid disinfectant has all the final sterilization factors of chlorine-containing disinfectants. The final active ingredient of chlorine-containing disinfectants dissolved in water is hypochlorous acid. Hypochlorous acid has been discovered for more than a century, but due to its extreme instability, it has been unable to achieve commercialization and large-scale application. In different hypochlorous acid preparation processes, hundreds of derivatives are generated, and the quality of hypochlorous acid products on the market is uneven, the stability is difficult to be reliable, and the product quality is difficult to be guaranteed. SUMMARY

[0003] The present application provides a hypochlorous acid disinfectant, a preparation method and application thereof, which generates a hypochlorous acid aqueous solution by electrolyzing a 5% NaCl aqueous solution, generates hydrogen ions and hydroxyl radicals and forms a stable binary complex structure, can improve the stability of the hypochlorous acid disinfectant through the positive and negative double electrodes formed by the binary complex structure, prolong the product shelf life, and improve the microbial killing effect. The present application specifically adopts the following technical solutions.

[0004] First, in order to achieve the above-mentioned purpose, a hypochlorous acid disinfectant is provided, which is obtained by electrolyzing a NaCl aqueous solution, and in the hypochlorous acid disinfectant, hydroxyl radicals (·OH-) and hydrogen ions (H+) form a binary complex structure and stably coexist for a long time to achieve a sterilization effect at a very low concentration. The hypochlorous acid disinfectant according to any one of the above further adds a stabilizer including the following components in a mass ratio of 0.5-1%: sodium phosphate 1-4 parts, disodium hydrogen phosphate 1-3 parts, sodium dihydrogen phosphate 1-3 parts, sodium silicate 3-7 parts, sodium polyphosphate 3-7 parts, phosphazene 1-3 parts, and sodium bromide 2-5 parts; wherein the hypochlorous acid and the hydroxyl radical form disinfecting factors respectively and synergistically.

[0005] The hypochlorous acid disinfectant according to any one of the above, wherein the concentration of the NaCl aqueous solution for electrolysis to prepare the hypochlorous acid disinfectant is 0.5-5%, and the solvent is purified water.

[0006] The hypochlorous acid disinfectant according to any one of the above, wherein the concentration of the hypochlorous acid in the hypochlorous acid disinfectant is between 0.005 ppm and 3000 ppm.

[0007] To achieve the above object, the application further provides a preparation method of hypochlorous acid disinfectant, which, in addition to normal continuous electrolysis, also adds reverse electrolysis before initial electrolysis, so as to better activate the electrode, prolong the electrode life, improve the consistency of the batch electrode potential, and improve the product quality. The specific steps of the preparation method include: dissolving iodine-free refined edible salt in purified water to prepare a NaCl aqueous solution with a concentration ranging from 0.5 to 5%; first performing reverse electrolysis, and then performing continuous electrolysis of the NaCl aqueous solution in four series-connected electrolytic cells under a constant current at an oxidation-reduction potential of -60 to 2000 MeV to obtain electrolytic water; diluting the electrolytic water with purified water to a calibrated concentration to obtain the hypochlorous acid disinfectant.

[0008] The preparation method as described in any of the above, wherein the concentration of the NaCl aqueous solution ranges from 0.5 to 5%; and the calibrated concentration is a hypochlorous acid concentration ranging from 0.005 ppm to 3000 ppm.

[0009] The preparation method as described in any of the above, wherein, during the electrolysis process, the anode reaction includes: water electrolysis to generate hydrogen ions (H + ), NaCl electrolysis to generate chlorine gas (Cl2), and reaction of the chlorine gas (Cl2) with water to generate hypochlorous acid (HOCl).

[0010] The preparation method as described in any of the above, wherein, during the electrolysis process, the cathode reaction includes: water electrolysis to generate hydroxyl radicals (·OH - ), and reaction of NaCl with hydroxide ions to generate sodium hydroxide (NaOH). The preparation method as described in any of the above, wherein, at the electrolysis end point, the hydrogen ions (H + ) and the hydroxyl radicals (·OH - ) form a binary complex structure and stably coexist for a long time; and further adding a stabilizer including the following components to the hypochlorous acid disinfectant at a mass ratio of 0.5 to 1% after diluting the electrolytic water to a calibrated concentration: sodium phosphate 1 to 4 parts, disodium hydrogen phosphate 1 to 3 parts, sodium dihydrogen phosphate 1 to 3 parts, sodium silicate 3 to 7 parts, sodium polyphosphate 3 to 7 parts, phosphazene 1 to 3 parts, and sodium bromide 2 to 5 parts.

[0011] The hypochlorous acid disinfectant obtained by the preparation method as described in any of the above, wherein the hydrogen ions (H +) form a specific mode and long-term stable coexistence, respectively form a disinfectant factor, with the hypochlorous acid produced synergies, greatly improve the titer of the disinfectant factor of the product, even very low concentration also has good bactericidal performance, truly to low concentration and high efficiency. Its application range includes at least one of the following: in the aquatic water, dilute the product 300,000 times (only 0.005 ppm) still have 24 hours of 4 log of bacteriostatic effect. In the disinfectant solution, hypochlorous acid and hydroxyl radical synergies, break the cell membrane, degrade viral nucleic acid, DNA, RNA; hypochlorous acid itself will produce hydroxyl radicals, instantaneously kill bacteria, and because the existence time of hydroxyl is very short, the disinfectant will not produce harmful; the hypochlorous acid disinfectant solution into the aquatic water can be used to degrade viruses, formaldehyde, toluene, xylene, degrade vomitoxin, reduce and remove heavy metal ions in water, that is, by reducing heavy metal ions to metal atoms, thereby removing heavy metal pollution; also can be used to penetrate the cell wall, damage the bacterial and microbial cell membrane, destroy the intracellular protein substances and nucleotides in the cell nucleus, destroy the electron transport chain of microorganisms, affect the enzyme system in biological metabolism, and react with microorganisms to generate oxygen-containing active small molecule substances and chloramine, kill pathogens and microorganisms in the body and outside of pigs, poultry and livestock; prepare mouthwash, kill and remove Helicobacter pylori in the oral cavity. The mouthwash with hypochlorous acid as the main functional ingredient has high antibacterial and antibiofilm effects on Helicobacter pylori and low cytotoxicity, and can be used as a conventional treatment auxiliary drug for patients infected with Helicobacter pylori

[0012] Beneficial effects

[0013] The hypochlorous acid disinfectant solution, its preparation method and application provided by the present application adopt a special process, generate a hypochlorous acid aqueous solution by electrolyzing 0.5-5% NaCl aqueous solution, and utilize the synergistic effect of hypochlorous acid and hydroxyl radicals in the hypochlorous acid aqueous solution prepared by the present application to directly break the cell membrane and degrade DNA and RNA to achieve flash sterilization and broad-spectrum sterilization. In the hypochlorous acid disinfectant solution of the present application, the hydroxyl and hydrogen ions (H + ) can form a specific mode of binary composite structure and long-term stable coexistence to overcome the problem of extreme instability of traditional hypochlorous acid disinfectant solution. The disinfectant factor in the disinfectant solution of the present application is efficient, safe and stable, has no irritation to skin and mucous membrane, and is friendly to the environment. After using the disinfectant of the present application for environmental disinfection, viral nucleic acid, DNA and RNA can be degraded, which is an international innovation. This feature can fill the gap in the domestic and foreign markets and greatly reduce the probability of virus transmission.

[0014] The present application generates an aqueous hypochlorous acid solution by electrolyzing 0.5-5% NaCl aqueous solution, and the concentration of the hypochlorous acid is from 0.005ppm to 3000ppm. The hypochlorous acid can generate synergistic effect with hydroxyl radicals at the redox potential of-60-2000MeV, directly break the cell membrane, and degrade DNA and RNA. The present application overcomes the problem that it is unable to identify whether nucleic acid has been degraded after environmental disinfection using traditional disinfectants. In the hypochlorous acid disinfectant of the present application, hydroxyl and hydrogen ions (H + ) form a specific mode and stably coexist for a long time, respectively form disinfection factors, and generate synergistic effect with the hypochlorous acid to form a new type of disinfectant with nucleic acid degradation function, which overcomes the problem of extreme instability of the hypochlorous acid preparation, has a long shelf life, can realize real product sales, and is also beneficial to QA (QUALITY ASSURANCE, quality assurance) and QC (QUALITY CONTROLLER, quality control). The novelty search report shows that the hypochlorous acid disinfectant provided by the present application has not been reported in the literature at home and abroad.

[0015] The hypochlorous acid disinfectant provided by the present application not only has high efficiency in sterilization, but also has the characteristics of green environmental protection and no residue. It can remove harmful substances such as ammonia, formaldehyde, toluene, xylene, etc. After the national environmental protection product quality supervision and inspection, the hypochlorous acid disinfectant prepared by the present application can not only decompose organic matter, but also produce organic acids needed by the breeding industry, reduce heavy metal ions by using organic acids, convert them into atoms, and thus remove heavy metal pollution. At the same time, it realizes antibiotic-free aquaculture and protects the environment on which we depend.

[0016] The hypochlorous acid of the present application is applied to aquatic water bodies. After inspection by Jiangsu Freshwater Aquatic Institute, after diluting 1500ppm concentrated solution by 300000 times (only 0.005ppm), there is still 24 hours of 4log bacteriostatic effect.

[0017] In addition, the hypochlorous acid disinfectant of the present application can also be configured as a mouthwash with hypochlorous acid as the main functional component, which is used for antibacterial treatment of Helicobacter pylori. The hypochlorous acid of the present application can realize disinfection and killing effect by resisting biofilm effect, and has no cytotoxicity, which provides the possibility of using hypochlorous acid as a conventional treatment adjuvant for patients infected with Helicobacter pylori. The hypochlorous acid disinfectant prepared by the present application can be conveniently carried after being configured as a mouthwash, has the characteristics of low price, low concentration and high efficiency, and pH close to oral mucosa. After a large number of personnel use, the treatment effect on Helicobacter pylori infection is remarkable.

[0018] Other features and advantages of the present application will be set forth in the following description, and in part will become apparent from the description, or will be learned by practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0019] The accompanying drawings are provided to further illustrate the present application and form part of the specification. Together with the embodiments of the present application, they serve to explain the present application but do not constitute a limitation thereof. In the drawings:

[0020] Figure 1 The hydroxyl radicals (·OH) obtained during the preparation of hypochlorous acid disinfectant in this application are... - ) and hydrogen ions (H + A schematic diagram of the formation of a binary composite structure;

[0021] Figure 2 This is a schematic diagram illustrating the inhibitory effect of the chloric acid disinfectant of this application on ASFV at 20°C.

[0022] Figure 3 This is a process flow diagram for preparing hypochlorous acid disinfectant solution according to this application;

[0023] Figure 4 This is a schematic diagram of the electrolysis process under one implementation method of this application. Detailed Implementation

[0024] To make the objectives and technical solutions of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the described embodiments of this application without creative effort are within the scope of protection of this application.

[0025] Those skilled in the art will understand that, unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. It should also be understood that terms such as those defined in general dictionaries should be understood to have the meaning consistent with their meaning in the context of the prior art, and should not be interpreted in an idealized or overly formal sense unless defined as herein.

[0026] Example 1

[0027] In this embodiment, 0.8 kg of NaCl was weighed and dissolved in water to obtain 160 kg of solution;

[0028] The above NaCl aqueous solution is continuously electrolyzed with a constant current. The reaction equation is as follows:

[0029] 2NaCl + 2H₂O = Electrolysis = Cl₂ + H₂ + 2NaOH

[0030] Cl₂ + H₂O → HCl + HClO

[0031] The electrolysis reaction simultaneously generates Figure 1 hydrogen ions (H + ) and hydroxyl radicals, forming a stable binary complex structure, similar to a yin-yang doublet. The binary complex structure formed by hydroxyl radicals (·OH - ) and hydrogen ions (H + ) can stably coexist to improve the stability of the hypochlorous acid prepared in the above manner.

[0032] In addition, in the disinfectant solution prepared, the hypochlorous acid can also form disinfecting factors with hydroxyl radicals, respectively, to synergize.

[0033] The hypochlorous acid disinfectant prepared in the above step was subjected to the following test to determine the effective concentration of the disinfectant on the degradation of nucleic acid at different temperatures:

[0034] Under the environment of a 37°C oven, an equal amount of porcine pseudorabies live vaccine was smeared in a clean tray, and the inoculation method was cross-line method, with each line being 10 cm long, and a total of 10 lines. The hypochlorous acid disinfectant (concentrated to 1500 ppm), the hypochlorous acid disinfectant stock solution (500 ppm), the 1:8 dilution (56 ppm), the 1:16 dilution (29 ppm), the 1:32 dilution (15 ppm), and the 1:64 dilution (11 ppm) prepared in the foregoing step were respectively poured into the tray smeared with the vaccine, and then left to stand at room temperature for 10 min, 15 min, 30 min, and 45 min (see Table 1 for details). Immediately after the timing ended, the disinfectant in the tray was poured out and left to dry. Then, sampling detection was performed on the surface of the tray. A cotton swab was soaked and eluted in 1 mL of PBS in a 5 mL centrifuge tube, and the eluate was subjected to pseudorabies virus nucleic acid detection.

[0035] The above-mentioned operation was operated at 4°C to simulate the climate in winter and summer to explore the effect of temperature on the disinfectant, and the results shown in Table 2 were obtained.

[0036] Table 1-Disinfection concentration and time in a 37°C environment

[0037] Concentration Disinfection time 1 Disinfection time 2 Disinfection time 3 Disinfection time 4 1500 ppm 10 min 15 min 30 min 45 min 500 ppm 10 min 15 min 30 min 45 min 56 ppm 10 min 15 min 30 min 45 min 29 ppm 10 min 15 min 30 min 45 min 15 ppm 10 min 15 min 30 min 45 min 11 ppm 10 min 15 min 30 min 45 min

[0038] Table 2-Disinfection concentration and time in a 4°C environment

[0039] Concentration Disinfection time 1 Disinfection time 2 Disinfection time 3 Disinfection time 4 1500 ppm 10 min 15 min 30 min 45 min 500 ppm 10 min 15 min 30 min 45 min 56 ppm 10 min 15 min 30 min 45 min 29 ppm 10 min 15 min 30 min 45 min 15 ppm 10 min 15 min 30 min 45 min 11 ppm 10 min 15 min 30 min 45 min

[0040] An equal amount of porcine pseudorabies live vaccine was smeared in a clean tray, and the inoculation method was cross-line method, with each line being 10 cm long, and a total of 10 lines. Next, three methods of spraying, soaking, and wiping were used for testing.

[0041] Spray method: Use a spray bottle to spray the effective concentration of disinfectant on the crosshatched line, respectively at 10 min, 15 min, 30 min, 45 min four time periods on four trays smeared with live porcine pseudorabies vaccine sampling detection, cotton swab in 5 mL centrifuge tube with 1 mL PBS immersion elution, the eluent for pseudorabies virus nucleic acid detection.

[0042] Immersion method: Pour 50 mL of effective concentration of disinfectant into four trays smeared with live porcine pseudorabies vaccine, respectively at 10 min, 15 min, 30 min, 45 min four time periods each on a tray for sampling detection, cotton swab in 5 mL centrifuge tube with 1 mL PBS immersion elution, the eluent for pseudorabies virus nucleic acid detection.

[0043] Wipe method: the effective concentration of disinfectant soaked gauze wipe the tray twice, dry 10 min, 15 min, 30 min, 45 min four time periods each on a tray for sampling detection, sampling cotton swab in 5 mL centrifuge tube with 1 mL PBS immersion elution, the eluent for pseudorabies virus nucleic acid detection can obtain table 3 results.

[0044] Table 3 - the effective concentration of disinfectant on nucleic acid degradation at different temperatures

[0045]

[0046]

[0047]

[0048] From the above experiments, it is known that the 37℃ environment slightly enhances the degradation of pseudorabies virus nucleic acid by the hypochlorous acid disinfectant provided in the embodiment compared with the 4℃ environment, which guides us that the disinfection in summer is more effective than in winter. Moreover, prolonging the disinfection time and increasing the concentration of disinfectant can effectively enhance the degradation of pseudorabies virus nucleic acid by the disinfectant. In addition, the results also found that the ratio of disinfectant to water above 56 ppm has a significant effect on the degradation of pseudorabies virus nucleic acid. Therefore, it is recommended to configure the final concentration to be above 56 ppm when actually applying disinfection.

[0049] Spray method and wipe method may not be able to completely degrade nucleic acid in a certain time. The reason is that the small amount of droplets sprayed by the spray method is not enough to degrade the pseudorabies virus nucleic acid, and although the wipe method wipes twice to make the disinfectant fully contact with the vaccine, the disinfectant dries quickly and does not have enough time to react with the vaccine. The two methods may result in unsatisfactory wiping disinfection effect. It is recommended to choose the method of excessive liquid immersion disinfection to disinfect the articles in practical application.

[0050] The test data of the embodiment show that:

[0051] The hypochlorous acid disinfectant solution obtained according to the preparation method of the embodiment can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of 4℃, dilution ratio ≤9 times, and action time of 10 minutes; and can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of dilution ratio ≤17 times and action time of 45 minutes;

[0052] The hypochlorous acid disinfectant solution obtained according to the preparation method of the embodiment can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of 37℃, dilution ratio ≤9 times, and action time of 10 minutes; and can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of dilution ratio ≤17 times and action time of 15 minutes;

[0053] The concentrated solution and the original solution of the hypochlorous acid disinfectant solution obtained according to the preparation method of the embodiment can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of room temperature, and action time of 10 minutes when soaking, spraying and wiping the articles;

[0054] The hypochlorous acid disinfectant solution obtained according to the preparation method of the embodiment can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of room temperature, dilution ratio ≤9 times, and action time of 10 minutes when soaking the articles; and can effectively degrade the porcine pseudorabies virus nucleic acid under the condition of spraying the articles, and action time of 45 minutes.

[0055] Example 2

[0056] In the preparation process of the hypochlorous acid disinfectant solution, 8 kg of non-iodized refined edible salt is dissolved in 160 kg of purified water to prepare a NaCl aqueous solution with a concentration range of 5%;

[0057] Then, reverse electrolysis is performed. The reverse electrolysis is to reverse the positive and negative electrodes of normal electrolysis, connect the positive electrode of the power supply to the cathode in the subsequent electrolysis step, and connect the negative electrode of the power supply to the anode in the electrolysis step to better activate the electrode, prolong the service life of the electrode, improve the consistency of the batch electrode potential, and improve the product quality. After a period of reverse electrolysis, the NaCl aqueous solution is continuously electrolyzed in a 4-stage electrolytic cell system connected in series under a constant current at a redox potential of -60-2000 MeV to obtain electrolytic water. Figure 4 The 4-stage electrolytic cell system uses a high-speed pump to pump the prepared NaCl aqueous solution into the electrolytic cell, and sequentially passes through the 4-stage electrolytic cell for continuous electrolysis for 15-30 minutes. During the electrolysis process, the anode reaction includes:

[0058] 2H2O→4H + +O2↑+4e -

[0059] 2NaCl→Cl2↑+2e - +2Na +

[0060] Cl2+H2O→HCl+HOCl

[0061] In the electrolysis process, the anode reaction includes:

[0062] 2H2O + 2e - → 2OH - + H2↑

[0063] 2NaCl + 2OH - → 2NaOH + 2Cl -

[0064] The main component of refined edible salt (without iodine) is sodium chloride, which is dissolved in purified water to form brine, and then Cl- is provided in the electrolysis process, and Cl- is the main source of HClO. The content of sodium chloride affects the effective chlorine content of electrolytic water after electrolysis.

[0065] Sodium chloride is easily soluble in water, and purified water is a good solvent. In the electrolysis process, purified water is used as a medium to generate a constant current in the electrolytic cell for continuous electrolysis.

[0066] The binary complex structure formed by hydrogen ions (H + ) and hydroxyl radicals (·OH - ) in electrolytic water is used as the electrolysis endpoint. After the hydrogen ions (H + ) and hydroxyl radicals (·OH - ) coexist stably for a long time after electrolysis, purified water is added to the electrolytic water to dilute it to a calibrated concentration of 0.005 ppm to 3000 ppm, obtaining a hypochlorous acid disinfectant.

[0067] The above step-by-step continuous electrolysis method can repeatedly mix the hydrogen ions (H + ) and hydroxyl radicals (·OH - ) generated by electrolysis to form a stable binary complex structure, so that the hydroxyl radicals (·OH - ) and hydrogen ions (H + ) form a stable coexistence similar to yin and yang. In the electrolyte, hypochlorous acid can form disinfecting factors with hydroxyl radicals, respectively, and synergistically. The purity and stability of the hypochlorous acid in the chlorine-containing disinfectant (hypochlorous acid disinfectant) prepared by the above method are higher than those of the traditional single electrolysis method.

[0068] In view of the existing aquaculture mode, especially the white shrimp farming water body in South America, groundwater is used in the farming process, which causes heavy metal residues to exceed the standard. Nitrite is also produced in the process of aquaculture, which pollutes the water body and the farmed animals. Hemolysin is widely present in the ecological farming of Vibrio, and if not properly controlled, a large number of shrimps will die, causing significant economic losses. Therefore, the hypochlorous acid disinfectant prepared by the foregoing method is applied to the farming water body to test the degradation capacity of the hypochlorous acid disinfectant of the present application to viral nucleic acid, formaldehyde, toluene, dimethylbenzene, the degradation capacity to vomit toxin, and the removal capacity to heavy metals in the water body:

[0069] Test strain: The strain isolated from white shrimp in South America is stored in a laboratory -80C refrigerator.

[0070] Two disinfectants are selected, namely 1 and 2. Disinfectant 1 mainly contains hypochlorous acid prepared in this embodiment; disinfectant 2 mainly contains hydrogen peroxide provided by SANOSIL Company in Switzerland.

[0071] Culture medium and reagent: LB nutrient broth medium, TCBS agar medium plate, TCBS liquid culture medium and sterile physiological saline are provided by Guangzhou Huankai Biotechnology Co., Ltd.

[0072] Test method:

[0073] 1-Strain recovery and purification

[0074] The frozen tube containing the bacterial solution is thawed at room temperature, and LB nutrient broth medium is added. It is incubated at 37°C for 24 hours. It is inoculated and streaked on TCBS culture medium at 37°C for 24 hours. Blue-green single colonies are picked and streaked on TCBS culture medium. After repeating the test for 3 times, the blue-green single colonies are inoculated on TCBS liquid culture medium and incubated at 37°C for 24 hours for standby.

[0075] 2-Preparation of bacterial suspension

[0076] The TCBS culture solution containing bacteria is centrifuged at 4°C, and the supernatant is discarded. Sterile physiological saline is added, mixed and centrifuged, and the supernatant is discarded. After repeating 3 times, sterile physiological saline is added to prepare a bacterial suspension and count. It is stored in a 4°C refrigerator for standby.

[0077] 3-Bactericidal experiment

[0078] Add 1 mL of bacterial suspension to sterile tube #1. Add 900 μL of sterile physiological saline to each of sterile tubes #2-#8. Add 100 μL of bacterial suspension from tube #1 to tube #2, and continue serial dilution until tube #7 is discarded. Do not add any to tube #8. Then add 100 μL of 0.050 mg / L disinfectant solution 1 to each tube. After mixing and standing for 24 hours, add 500 μL to TCBS medium and incubate at 37°C for 24 hours. Observe for bacterial growth. Perform the same test on other concentrations of disinfectant solution 1 and all concentrations of disinfectant solution 2. Record the test results.

[0079] The experiment was conducted in three Litopenaeus vannamei ponds at a Litopenaeus vannamei farm in Nantong. Water samples were collected from the ponds using sterile equipment and numbered (No. 1 to No. 3). Each pond had two experimental groups and a control group. The experimental groups were treated with disinfectant solution 1 and disinfectant solution 2, respectively, resulting in final concentrations of 0.005 mg / L and 1.000 mg / L. The control group received no disinfectant. The ponds were kept at 37℃ for 24 hours. The total bacterial count was measured, and the Vibrio parahaemolyticus suspension count was recorded as 2.81 x 10⁻⁶. 11 CFU / L.

[0080] The bactericidal results of disinfectant solution 1 and disinfectant solution 2 against Vibrio parahaemolyticus showed that when the concentration of Vibrio parahaemolyticus was 2.81 x 10⁻⁶, the bactericidal effect was significantly reduced. 11 The minimum bactericidal concentration for disinfectant solution 1 at CFU is 0.005 mg / L, and the minimum bactericidal concentration for disinfectant solution 2 is 1.000 mg / L.

[0081] The antibacterial results of disinfectants on Litopenaeus vannamei ponds showed that disinfectants 1 and 2, while killing Vibrio parahaemolyticus at the lowest concentration, also significantly inhibited the total bacterial count in the pond water.

[0082] Table 4 - Antibacterial effect of disinfectant

[0083]

[0084] Example 3

[0085] This embodiment uses Figure 3 As shown, first weigh 1.6 kg of uniodized refined edible salt, add 160 kg of purified water to dissolve and prepare a 1% NaCl aqueous solution;

[0086] Then, the NaCl aqueous solution is continuously electrolyzed with a constant current to obtain electrolyzed water;

[0087] After electrolysis, 500 kg of purified water is added to the electrolyzed water to obtain the disinfectant solution.

[0088] As the concentration of the original solution of the present formula is high, the solution needs to be further diluted with purified water when it is marketed and applied. To ensure the stability of the active ingredients after dilution, the present embodiment further adds a core stabilizer to the disinfectant solution prepared by adding 500 kg of purified water to the electrolytic water, so as to consolidate the stability of the diluted solution and avoid the influence of the active ingredients of the disinfectant on the subsequent dilution process.

[0089] Specifically, after the electrolytic water is diluted to the designated concentration, the stabilizer including the following components is further added to the hypochlorous acid disinfectant solution at a mass ratio of 0.5-1%:

[0090] 1-4 parts of sodium phosphate, 1-3 parts of disodium hydrogen phosphate, 1-3 parts of sodium dihydrogen phosphate, 3-7 parts of sodium silicate, 3-7 parts of sodium polyphosphate, 1-3 parts of phosphazene, and 2-5 parts of sodium bromide.

[0091] Thus, the above disinfectant can further ensure the application effect after being diluted by the user when it is marketed, overcome the problem of extreme instability of the traditional hypochlorous acid preparation, ensure a long shelf life, and truly realize productized sales.

[0092] The above stabilizer can be added to the hypochlorous acid disinfectant solution in the following manner:

[0093] First, sodium silicate and sodium polyphosphate are dissolved in 10 parts of water and poured into the diluted disinfectant solution, then sodium phosphate is added and stirred, followed by the addition of sodium bromide and phosphazene, and finally disodium hydrogen phosphate and sodium dihydrogen phosphate are added, and constant slow stirring is performed to dissolve all the additives, so that the total amount of the additives accounts for 0.5-1% of the disinfectant solution, and the amount of inorganic salts accounts for 15-25% of the disinfectant solution.

[0094] In the above stabilizer, sodium phosphate interacts with hypochlorous acid to produce trisodium phosphate chloride. It is a very ideal, rare, non-toxic, high-efficiency, and rapid cleaning and disinfectant with both the decontamination and washing functions of sodium phosphate and the disinfection performance of hypochlorous acid, and can also remove silicate scale.

[0095] Disodium hydrogen phosphate and sodium dihydrogen phosphate are strong buffering agents, which can effectively stabilize the liquid phase in the medium within a certain pH value range to control and maintain a stable pH value change range.

[0096] Sodium silicate can play the roles of stabilizing, slow-release, and reacting with water-soluble magnesium salts to form a precipitate, and can effectively reduce the tendency of the concentration of available chlorine to decrease.

[0097] Sodium bromide is an excellent additive that can prevent the disproportionation reaction of hypochlorous acid during reversible decomposition, and the decrease rate of the concentration of available chlorine tends to slow down with the increase of the amount of sodium bromide.

[0098] Phosphazene utilizes the stability of aromatic ring and the reversible substitution principle of halogen atoms. The difference in nucleophilic action of Cl-and OCl-to phosphorus atoms changes the internal environment of disinfectant solution in reversible nucleophilic substitution, reduces the concentration of OCl-in disinfectant solution, and indirectly stabilizes the effective chlorine concentration of disinfectant solution.

[0099] To further verify the stabilizing effect of the above stabilizer on hypochlorite disinfectant, the following comparative test was also conducted:

[0100] Take an appropriate amount of disinfectant solution that has been appropriately diluted without additives, and measure the effective chlorine content of 572 mg / L. Add the corresponding number of stabilizers in the proportion of the group according to the proportion, and divide into sealed light-proof containers.

[0101]

[0102] According to the stability test guidelines of the 2002 edition of "Disinfection Technical Specifications" and the 2020 edition of "Chinese Veterinary Pharmacopoeia", the content was measured after being placed at 54℃ for 14 days and 60℃ for 10 days under two conditions, and the following table results were obtained.

[0103] Grouping 54°C 14 days 60°C 10 days A 341 322 B 128 109 C 338 339 D 312 309 E 310 321 F 387 379 AB 223 216 AC 412 428 CD 473 465 DEF 421 417 ABF 453 448 ABCDEF 524 518

[0104] To verify whether the addition of stabilizer in a proportion of 0.5-1% will affect the properties or stabilizing effect of the disinfectant, the following comparative test was also conducted in this embodiment:

[0105]

[0106]

[0107] For low-temperature hypochlorite disinfectant:

[0108] In this test, the stabilizer can be prepared in the low-temperature hypochlorite disinfectant according to the following steps: first dissolve the antifreeze in the diluted disinfectant, then dissolve sodium silicate and sodium polyphosphate in 10 parts of water and pour into the disinfectant, then mix well after adding sodium phosphate, then add sodium bromide and phosphazene in turn, and finally add disodium hydrogen phosphate and sodium dihydrogen phosphate, and continuously slowly stir to dissolve.

[0109]

[0110] Take an appropriate amount of disinfectant solution that has been appropriately diluted without additives, and measure the effective chlorine content of 572 mg / L. Add the corresponding number of stabilizers in the proportion of the group according to the proportion, and divide into sealed light-proof containers, and measure the content after being placed at 25℃ for 4 months and 30℃ for 4 months under two conditions according to the stability test guidelines of the 2002 edition of "Disinfection Technical Specifications" and the 2020 edition of "Chinese Veterinary Pharmacopoeia".

[0111]

[0112]

[0113] To verify whether the proportion of stabilizer added does not meet 0.5-1% will affect the properties or stability of the disinfectant, the following comparative test is also carried out in this embodiment:

[0114]

[0115] The comparative test shows that the addition amount of the antifreeze should not be less than 21.5% of the disinfectant. Too low addition amount will cause the low-temperature disinfectant to freeze at-20℃ and affect the disinfection effect, and too much will increase the production cost.

[0116] From the above comparative data, it can be seen that each component of the stabilizer in this application has only weak or no effect on the stability of the disinfectant when used alone, but after they are compounded in a certain order and proportion, they can synergize with each other, significantly improve the stability of the flash water disinfectant, and make the product can be used in-20℃ environment to achieve effective disinfection.

[0117] Therefore, after the firefighters purchase the disinfectant with the above stabilizer, they can dilute it according to the needs, and use the prepared solution for the prevention and control of African swine fever and other animal diseases, so as to overcome the contradiction between the safety and efficiency of the existing disinfectant in the veterinary drug market.

[0118] Specifically, compared with the traditional acid hypochlorous acid, the hypochlorous acid disinfectant obtained by the preparation method of this embodiment has the following characteristics: 1. High concentration, after inquiry, the concentration of 432 hypochlorous acid products recorded on the national disinfection product online record information service platform is between 50-200ppm, while the effective chlorine content of the hypochlorous acid disinfectant obtained in this embodiment reaches 500ppm, the effective concentration is higher, the transportation and packaging cost is greatly reduced; 2. Good stability, after detection, the hypochlorous acid disinfectant prepared in this embodiment has a stability of 1 year (even the 1500ppm concentrated solution has a stability period of more than four months), good product hygiene quality, and long shelf life; 3. No irritation to human skin and mucous membrane, no toxicity and harm to human body, no toxicity by mouth, no irritation, negative mutagenicity, lower use concentration, no toxic and harmful chemical residues, no environmental pollution, and the main metabolites are salt and water.

[0119] In other implementation manners, the chemical equilibrium can be moved to the right side by increasing the H+ concentration and other ways during the electrolytic production of hypochlorous acid, to further reduce the concentration of NH3. The valence of Cl element in HClO is +1, and the stable valence of Cl element is-1, so it has strong oxidizing property.

[0120] No matter which preparation method is used, the hypochlorous acid (HCIO) prepared has the ability to penetrate the cell wall, damage the cell membrane, release nucleic acids (DNA, RNA), proteins, and organelles of microorganisms such as bacteria, and affect the normal function of various biological enzyme systems (such as the oxidation and destruction of the -SH of phosphogluconate dehydrogenase), thereby achieving the purpose of killing pathogenic bacteria.

[0121] The mechanism of action of the hypochlorous acid prepared in this embodiment for killing microorganisms includes the following aspects: ① hypochlorous acid causes a certain degree of damage to the cell wall and cell membrane of microorganisms; ② hypochlorous acid can damage a large amount of protein substances present in the cell, and can also cause damage to nucleotides in the cell nucleus; ③ hypochlorous acid can seriously affect the metabolism of microorganisms by damaging the electron transport chain and various key enzymes in the metabolism of microorganisms, which plays a very important role in the process of killing microorganisms by hypochlorous acid; ④ hypochlorous acid can generate oxygen-containing active small molecule substances and chloramine after reacting with microorganisms, which can also have a killing effect on microorganisms.

[0122] In addition, due to the special process of the present application, the hydrogen ions (H + ) and hydroxyl groups OH- in the hypochlorous acid solution prepared in this embodiment can coexist for a long time. The hydroxyl radical (·OH) is an important active oxygen, which is formed by losing an electron from the hydroxyl ion (OH-). The hydroxyl radical has a very strong electron-accepting ability, that is, oxidation ability, with an oxidation potential of 2.8V, which is the second strongest oxidizing agent in nature after fluorine. Hypochlorous acid and hydroxyl radicals can produce a synergistic effect, directly penetrating the cell membrane and degrading DNA and RNA. The hydroxyl radical (OH-) and hydrogen ions (H + ) form a specific mode and coexist stably for a long time, respectively forming a disinfection factor, and producing a synergistic effect with hypochlorous acid, greatly improving the titer of the disinfection factor of the product. Even at a very low concentration, it has a good bactericidal performance, truly achieving low concentration and high efficiency, and the process temperature range is very wide, even at minus 20℃, it has a 5-log bactericidal performance. Clinical studies have shown that the disinfection effect on pigs, poultry, and livestock is significant. In aquatic water bodies, the intermediate product of the process flow of the product is diluted by 300,000 times (only 0.005ppm), and still has a 4-log bacteriostatic effect for 24 hours.

[0123] When it is used for the killing and prevention of African swine fever virus, the following steps are performed to obtain Figure 2 data.

[0124] Test cells: primary porcine alveolar macrophages (PAMs).

[0125] The African swine fever virus strain: ASFV / China / GZ201801 was provided by the National African Swine Fever Regional Laboratory (Guangzhou), and the CT value of the virus stock solution was 20 by fluorescence quantitative PCR.

[0126] The hypochlorous acid disinfectant solution and sodium thiosulfate (10 g / L) prepared in the preceding step were diluted multiple times with purified water, and the undiluted or equally diluted samples and sodium thiosulfate were mixed at a volume ratio of 1:2 for standby use.

[0127] Test grouping: 3 replicates per group:

[0128] Disinfectant + cell culture: observe whether the disinfectant has an effect on cell growth.

[0129] Test group: PAMs were plated in 24-well plates, and flash water brand hypochlorous acid disinfectant stock solution, 2-fold, 10-fold, and 50-fold diluted disinfectant solutions were added, respectively. After 2 hours of action, the disinfectant solution was removed, and cell maintenance medium was added, and the plates were incubated in a 37°C carbon dioxide incubator. The cell state was observed daily for 5 consecutive days.

[0130] Table 5 - Average CT value of ASFV detected by fluorescence quantification

[0131] 1 day 3 days 5 days Stock solution 38.29 38.27 38.17 2-fold dilution 38.5 37.49 38.1 10-fold dilution 38.18 38.17 36.78 50-fold dilution 38.17 38.3 37.56 Virus group 34.26 26.92 21.92 Inactivated virus group Not detected 38.17 37.77 Blank group 38.41 38.4 37.12

[0132] Figure 2 The data show that: (1) After three repeated tests, the stock solution of hypochlorous acid disinfectant prepared by the means of the present application can effectively inactivate African swine fever virus at 20°C for 1 minute. (2) After three repeated tests, the 2-fold, 10-fold, and 50-fold diluted solutions of hypochlorous acid disinfectant prepared by the means of the present application can effectively inactivate African swine fever virus at 20°C for 60 minutes.

[0133] When the hypochlorous acid disinfectant solution prepared in this example was diluted 500 times, it had no obvious effect on the growth of primary PAM cells, i.e., the cells grew normally. The smaller the dilution multiple of the flash water brand hypochlorous acid disinfectant solution, the greater the effect on cell growth, i.e., the cells grew abnormally, with atrophy, shedding, death, disintegration, etc. In the 2% FBS control group, the cells grew normally.

[0134] The ASFV inhibition test results of the tested samples showed that the hypochlorous acid disinfectant stock solution provided in this example, after reacting with ASFV at 20℃ for 1 min, followed by the addition of a neutralizing agent and inoculation into infected cells, was negative for fluorescence quantitative PCR after samples were collected on days 1, 3, and 5. The 2-fold, 10-fold, and 50-fold dilutions of the Flashwater brand hypochlorous acid disinfectant, after reacting with ASFV at 20℃ for 60 min, followed by the addition of a neutralizing agent and inoculation into infected cells, were all negative for fluorescence quantitative PCR after samples were collected on days 1, 3, and 5. (Average CT value >35 or not detected is considered negative; ≤35 is considered positive).

[0135] The following conclusions can be drawn from its practical application in farms for the disinfection and prevention of African swine fever virus:

[0136] By observing and comparing the changes in the pig herd before and after the use of the hypochlorous acid disinfectant prepared in this application, it can be found that after use, the number of pigs with fever and reduced feed intake in the experimental pig herd decreased, the mortality rate decreased, and the health and feed intake of the pig herd recovered.

[0137] Blood tests conducted after the experiment revealed that viremia persisted in the pig herd. Although spraying hypochlorous acid disinfectant on the pig environment (including the factory floor and the harmless collection vehicle) did not kill the virus in the pigs, it kept the pigs in a stable state, indicating that flash disinfectant can inhibit viral disease to some extent.

[0138] By conducting pre- and post-experimental disinfection of the dead pigs disposal vehicle with different disinfectants, and by sampling the vehicle, it was found that the hypochlorous acid disinfectant solution (diluted at a ratio of 1:100) proposed in this application has a good nucleic acid degradation effect on African swine fever virus.

[0139] The hypochlorous acid disinfectant prepared in this application is non-irritating, safe to use, and does not require gloves or protective masks. It is more human-friendly, does not damage the mucosal immune barrier of humans and poultry / livestock, and causes no stress. Furthermore, this hypochlorous acid disinfectant has a short action time and broad-spectrum bactericidal activity. Unlike traditional disinfectants, which cannot determine whether nucleic acids have degraded after environmental disinfection, the hypochlorous acid disinfectant prepared by the method described in this application can decompose viral nucleic acid fragments. A search revealed no previous reports of this method domestically or internationally (Technology Novelty Search Report: 202232B2514966). This characteristic fills a gap in the domestic and international markets and will significantly reduce the probability of virus transmission.

[0140] Example 4

[0141] This embodiment is in Figure 3 Based on the preparation method shown, after electrolysis, a mouthwash is prepared with the hypochlorous acid electrolyzed water obtained as the main functional component.

[0142] The prepared mouthwash is then filtered and bottled to obtain the finished product, which is used as an adjunct to the routine treatment of patients with Helicobacter pylori infection.

[0143] Helicobacter pylori is infectious, and its infection can cause stomach diseases. Antibiotic resistance has become common for the eradication of Helicobacter pylori. However, the quadruple drug for treating Helicobacter pylori has many side effects, such as common nausea, vomiting, abdominal pain, dark stool, even tar-like black stool, palpitation and other side effects. Studies have confirmed that the oral cavity is one of the channels for Helicobacter pylori to enter the stomach, and the oral cavity plays an important role in the transmission of Helicobacter pylori in the stomach. After Helicobacter pylori colonizes in the oral cavity, it may trigger oral diseases such as periodontitis under certain conditions, and at the same time, it also has an impact on the gastric mucosa, leading to difficult eradication, recurrence of gastrointestinal diseases in patients, and poor treatment effect. Oral Helicobacter pylori is closely related to related gastrointestinal diseases and is an important factor causing the recurrence of gastrointestinal diseases. Therefore, if the patient has Helicobacter pylori parasitism in the gastrointestinal mucosa and causes clinical symptoms, in addition to gastrointestinal-related examinations, attention should also be paid to oral Helicobacter pylori infection. Treating oral diseases is an important means to improve the prognosis and a key factor to improve the treatment effect of gastrointestinal diseases.

[0144] Miyabayashi et al. observed that patients carrying Helicobacter pylori in the oral cavity had a significantly increased risk of gastric Helicobacter pylori infection after receiving appropriate antibacterial therapy. Therefore, maintaining oral hygiene can be used as an alternative method to improve the success rate of treatment, and the use of mouthwash helps to maintain oral hygiene.

[0145] The effective component hypochlorous acid obtained by the preparation of the present embodiment is the most friendly disinfecting factor for the human body recognized by the World Health Organization (WHO), and the WHO plans to add hypochlorous acid to the list of essential drugs in 2021. In the present embodiment, the hypochlorous acid prepared by electrolysis is configured as a mouthwash with hypochlorous acid as the main component, and the effect of the hypochlorous acid on Helicobacter pylori is as follows:

[0146] When the effective chlorine concentration is 7.5-15 ppm, the hypochlorous acid has good bactericidal effect on Helicobacter pylori;

[0147] When the effective chlorine concentration is greater than 3.75 ppm, the hypochlorous acid has good bacteriostatic effect on Helicobacter pylori;

[0148] When the effective chlorine concentration is greater than 0.938 ppm, the hypochlorous acid has good inhibitory effect on Helicobacter pylori biofilm;

[0149] When the effective chlorine concentration is within 15 ppm, the hypochlorous acid does not show cytotoxicity and has high safety.

[0150] And, due to the special process adopted by the present application, the hydrogen ions (H +) and hydroxyl radicals can coexist for a long time. Hydroxyl radical (·OH) is an important active oxygen, which is formed by losing one electron from hydroxide (OH-). Hydroxyl radical has a strong electron-accepting ability, that is, oxidation ability, with an oxidation potential of 2.8V, which is the second strongest oxidizing agent in nature after fluorine. Hypochlorous acid and hydroxyl radicals can produce synergistic effect, directly penetrate the cell membrane, and degrade DNA and RNA. Hydroxyl radicals and hydrogen ions (H + ) form specific modalities and coexist stably for a long time, respectively form disinfection factors, and produce synergistic effect with hypochlorous acid, greatly improving the titer of the disinfection factor of the product. Even at a very low concentration, the product has good bactericidal performance, truly achieving low concentration and high efficiency, and the process temperature range is very wide. Even at minus 20℃, it has a 5-log bactericidal performance. It can completely remove Helicobacter pylori in the oral cavity and has clear auxiliary treatment effect.

[0151] In summary, the hypochlorous acid disinfectant provided in the present application has synergistic effect of hypochlorous acid and hydroxyl radicals, which can penetrate the cell membrane and degrade viral nucleic acid, DNA and RNA;

[0152] When it is put into aquaculture water, it can effectively degrade viruses, formaldehyde, toluene, xylene, vomitoxin, and remove heavy metals in water;

[0153] The product can also penetrate the cell wall, damage the bacterial and microbial cell membrane, destroy the intracellular protein substances and nucleotides in the cell nucleus, destroy the electron transport chain of microorganisms, affect the enzyme system in biological metabolism, and react with microorganisms to generate oxygen-containing active small molecule substances and chloramine, killing pathogenic bacteria and microorganisms in and out of the body of pigs, poultry and livestock;

[0154] Most of the hypochlorous acid disinfectant products and traditional hypochlorous acid disinfectants on the market are in the form of A and B agents for low-temperature disinfection. However, by selecting an appropriate concentration of electrolyte and improving the electrolysis process and stabilizer, the present application can obtain a stable hypochlorous acid disinfectant product in the form of an effective ingredient solution, which does not need to be mixed and matched. The hypochlorous acid disinfectant provided in the present application can meet the national requirements for low-temperature disinfectants, and has been successfully recorded on the official platform of the Health Commission, the National Disinfection Product Network (https: / / credit.jdzx.net.cn / xdcp). It is the only low-temperature hypochlorous acid disinfectant that does not use A and B agents and is successfully recorded on the information service platform of the National Disinfection Product Network. The hypochlorous acid disinfectant provided in the present application has stable composition, no irritation to skin and mucous membrane, and the concentration of effective chlorine decreases by less than 10% after being placed at 25±2℃ for 12 months. When it is prepared into a mouthwash, it can have good inhibitory effect on Helicobacter pylori biofilm and kill and remove Helicobacter pylori in the oral cavity.

[0155] The above merely describes the embodiments of the present application, which are specific and detailed, but should not be understood as a limitation on the patent scope of the present application. It should be noted that, for those skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application.

Claims

1. A method of preparing a hypochlorous acid disinfectant solution, characterized by the steps of The application relates to a method for preparing a hypochlorous acid disinfectant solution. The method comprises the following steps: Dissolving non-iodized refined edible salt in purified water to prepare a NaCl aqueous solution with a concentration ranging from 0.5% to 5%; Firstly, reverse electrolysis is carried out, and then the NaCl aqueous solution is continuously electrolyzed in four-stage electrolytic cells connected in series under a redox potential of-60-2000 MeV at a constant current to obtain electrolytic water; The four-stage electrolytic cell system pumps the prepared NaCl aqueous solution into the electrolytic cells through high-speed pumps and sequentially passes through the four-stage electrolytic cells, The electrolysis is continuously carried out for 15-30 minutes; Purified water is added to the electrolytic water to dilute the electrolytic water to a calibrated concentration, and the calibrated concentration is the concentration of hypochlorous acid, which ranges from 0.005 ppm to 3000 ppm, so as to obtain a hypochlorous acid disinfectant solution; The hypochlorous acid disinfectant solution further comprises a stabilizer with a mass ratio of 0.5-1% and the stabilizer comprises the following components: Sodium phosphate 1-4 parts, disodium hydrogen phosphate 1-3 parts, sodium dihydrogen phosphate 1-3 parts, sodium silicate 3-7 parts, sodium polyphosphate 3-7 parts, phosphazene 1-3 parts and sodium bromide 2-5 parts; 2. The production method according to claim 1, wherein In the hypochlorous acid disinfectant solution, hypochlorous acid and hydroxyl radicals form disinfecting factors respectively and have a synergistic effect.

3. The production method according to claim 1, wherein The concentration of the NaCl aqueous solution is 1%.

4. The production method according to claim 1, wherein During the electrolysis process, the anode reaction comprises: water electrolysis to generate hydrogen ions (H+), NaCl electrolysis to generate chlorine gas (Cl2), and reaction of the chlorine gas (Cl2) with water to generate hypochlorous acid (HOCl). During the electrolysis process, the cathode reaction comprises: water electrolysis to generate hydroxyl radicals (·OH-), and reaction of NaCl with hydroxide ions to generate sodium hydroxide (NaOH).

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