A pesticide composition and use thereof

Abstract

The present application belongs to the technical field of pesticide insecticide and fungicide, and discloses a kind of pesticide composition and its use, the pesticide composition includes active ingredient A, active ingredient B and active ingredient C, the active ingredient A is propiconazole, the active ingredient B is fluazinam, and the active ingredient C is a new nicotine insecticide.The pesticide composition of the present application can simultaneously prevent and treat plant diseases and insect pests, the action mechanism of each active ingredient is different, and after compounding, obvious synergistic effect is played, the persistence of the pesticide can be prolonged, the resistance of pathogenic bacteria and pests is delayed, and the crop is safe and friendly to the environment.

Description

Technical Field

[0001] This invention belongs to the field of pesticide sterilization and insecticidal technology, and specifically discloses a pesticide composition and its uses. Background Technology

[0002] Seed coating agents are pesticide formulations made from pesticide technical materials (insecticides, fungicides, etc., some also containing micronutrients, hormones, film-forming agents, dispersants, antifreeze agents, and other adjuvants). They can be applied directly or after dilution to the surface of seeds, forming a protective film with a certain strength and permeability. Unlike conventional seed soaking or dressing, when the agent is applied to the seed, it immediately solidifies into a film to form a seed coating. This seed coating swells upon contact with water in the soil and is almost not dissolved, ensuring normal seed germination and growth while the pesticide and fertilizer are released slowly. It has significant effects in killing pests, preventing seed-borne diseases and seedling diseases, improving seed germination rate, promoting seedling growth, improving crop quality, and increasing yield.

[0003] Prothioconazole is a triazole thiophene fungicide developed by Bayer. Its mechanism of action is to inhibit the demethylation at the 14-position of lanosterol, a precursor of sterols in fungi, or 2,4-methylenedihydrolanosterol. It is mainly used to control numerous diseases in cereal crops such as wheat, barley, rapeseed, peanuts, rice, and legumes. It has excellent control effects on almost all wheat diseases.

[0004] Fluopyram, internationally known as penflufen (CAS Registry Number 494793-67-8), is a succinate dehydrogenase inhibitor developed by Bayer. It inhibits mitochondrial function by interfering with the activity of Complex II in the mitochondrial respiratory electron transport chain. It exhibits systemic, preventative, and curative effects, with a long-lasting effect. Fluopyram is effective in controlling diseases caused by pathogens such as basidiomycetes and ascomycetes.

[0005] Neonicotinic insecticides are a class of highly effective, safe, and selective insecticides. They primarily work by selectively controlling nicotinic acetylcholinesterase receptors in the insect nervous system, blocking normal transmission in the central nervous system, thus causing paralysis and death in pests. Neonicotinic insecticides are effective in controlling pests of the orders Homoptera, Hemiptera, Coleoptera, and Lepidoptera. They are safe for mammals and the environment, and can be used for foliar application, as well as for soil and seed treatment.

[0006] This invention rationally combines prothioconazole, fluopyram, and neonicotinoid insecticides to study their safety on crop seedling emergence and their control effects on plant diseases and pests. It also determines the optimal ratio and dosage of seed coating agents, providing a scientific basis for screening safe and efficient seed coating agents for large-scale application in production. Summary of the Invention

[0007] Based on the above, the present invention aims to provide a pesticide composition that can simultaneously control plant diseases and pests with a single application, has a broad spectrum of fungicidal and insecticidal activity, significant efficacy, is safe for crops, and slows down the development and progression of pesticide resistance in pathogens and pests.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition comprising active ingredient A, active ingredient B and active ingredient C, wherein active ingredient A is prothioconazole, active ingredient B is fluopyram, and active ingredient C is a neonicotinoid insecticide.

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:15 to 15:1;

[0010] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:15, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, 10:1, or 15:1.

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:15 to 10:1;

[0012] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:15, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, or 10:1;

[0013] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:10 to 6:1;

[0014] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:10, 1:6, 1:3, 1:1, 3:1, or 6:1;

[0015] Furthermore, the neonicotinoid insecticide is any one of clothianidin, dinotefuran, imidacloprid, acetamiprid, or thiacloprid.

[0016] Furthermore, the total mass ratio of active ingredient A and active ingredient B to the mass ratio of active ingredient C is 1:25 to 25:1;

[0017] Furthermore, the total mass ratio of active ingredient A and active ingredient B to the mass ratio of active ingredient C is 2:45 to 16:5;

[0018] Furthermore, the total mass ratio of active ingredient A and active ingredient B to the mass ratio of active ingredient C is 2:35 to 16:5;

[0019] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total weight of active ingredient A, active ingredient B, and active ingredient C accounts for 0.5% to 80% of the total content of the pesticide composition.

[0020] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total weight of active ingredient A, active ingredient B, and active ingredient C accounts for 1% to 70% of the total content of the pesticide composition.

[0021] Furthermore, the pesticide composition, in addition to the active ingredient, also includes auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, warning colors, film-forming agents, synergists, and carriers.

[0022] The wetting agent is selected from one or more of the following: alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0023] The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0024] The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0025] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0026] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0027] Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0028] Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0030] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0031] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0032] Warning colors are selected from any one or more of the following: blue, green, red, purple, and yellow; and / or

[0033] The film-forming agent is selected from one or more of sodium carboxymethyl starch, cellulose derivatives (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), and polyacrylic acid; and / or

[0034] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0035] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0036] Furthermore, the pesticide composition is in the form of either a solid seed treatment formulation or a liquid seed treatment formulation.

[0037] Furthermore, the pesticide composition is formulated as any one of seed treatment dry powder, seed treatment dispersible powder, seed treatment liquid, seed treatment emulsion, or seed treatment suspension.

[0038] Furthermore, the pesticide composition is in the form of a seed treatment suspension.

[0039] The present invention also discloses the use of the pesticide composition described above for the prevention and control of plant diseases and / or pests.

[0040] Furthermore, the plants mentioned include corn, wheat, soybeans, rice, peanuts and / or cotton;

[0041] The rice diseases mentioned are rice bakanae disease, rice blast, rice sesame spot disease, rice sheath blight, rice false smut, rice bacterial blight and / or rice seedling rot;

[0042] The wheat diseases mentioned are wheat root rot, wheat rust, wheat powdery mildew, wheat leaf blight, wheat smut, wheat sheath blight, and / or wheat take-all disease;

[0043] The corn diseases mentioned are corn stalk base rot, corn head smut, corn silk smut, corn ear rot, corn seedling blight and / or corn sheath blight;

[0044] The cotton diseases mentioned are cotton wilt, cotton verticillium wilt, cotton damping-off, cotton anthracnose, cotton red rot, cotton black spot, cotton brown spot, cotton stem blight, cotton angular leaf spot and / or cotton powdery mildew;

[0045] The rice pests mentioned are rice thrips, rice stem borers, rice skippers, rice planthoppers, rice leaf rollers, and / or rice water weevils;

[0046] The wheat pests mentioned are wheat aphids, wheat midges, wheat spider mites, armyworms, and / or wireworms;

[0047] The corn pests mentioned are corn borer, corn aphid, cotton bollworm, cutworm and / or armyworm;

[0048] The cotton pests mentioned are cotton aphids, cotton bollworms, cotton spider mites, cotton thrips, cotton leafrollers, cotton burrs, and / or cutworms.

[0049] Furthermore, the plant is corn, and the corn disease is any one of corn stem base rot, corn sheath blight, corn silk smut, corn ear and kernel rot, corn seedling blight, corn rough dwarf disease, or corn crazy top disease.

[0050] The corn pests mentioned are any one of the following: two-spotted cutworm, cutworm, wireworm, corn aphid, armyworm, beet armyworm, bollworm, or corn borer.

[0051] Furthermore, the corn disease mentioned is corn stalk rot, and the corn pest mentioned is corn aphid.

[0052] The beneficial effects of this invention are as follows:

[0053] 1) The pesticide composition of the present invention broadens the spectrum of fungicide and insecticide, and can effectively slow down the emergence and development of pesticide resistance in pathogens and pests;

[0054] 2) The pesticide composition of the present invention can prevent both plant diseases and pests with a single application, reduce the dosage of pesticides, have a long-lasting effect, and reduce pesticide residues in crops. Detailed Implementation

[0055] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following preferred embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0056] Formulation preparation example:

[0057] Seed treatment suspension preparation process: The active ingredients, additives, and water are mixed and stirred evenly under high shear according to the formula, and then sand milled for 2.5 hours to make the average particle size reach 1-5 micrometers. Finally, thickener, preservative, and film-forming agent are added and shearing and stirring are continued to be uniform to obtain the seed treatment suspension.

[0058] Preparation Example 1: 29% Thiamethoxam·Prothioconazole·Fluoxaflutole Seed Treatment Suspension (25% + 1% + 3%)

[0059] Formula composition: 25% thiamethoxam, 1% prothioconazole, 3% fluopyram aniline, 3% fatty alcohol polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether phosphate salt, 0.5% sodium lignosulfonate, 1.5% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% organosilicon defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0060] Preparation Example 2: 32% Thiamethoxam·Prothioconazole·Fluoxaflutole Seed Treatment Suspension (30% + 1% + 1%)

[0061] Formula composition: 30% thiamethoxam, 1% prothioconazole, 1% fluopyram aniline, 2% fatty amine polyoxyethylene ether, 4% glycerol fatty acid ester polyoxyethylene ether phosphate, 3% polyvinyl alcohol, 0.2% xanthan gum, 4% rose red pigment, 4% glycerol, 0.5% magnesium aluminum silicate, 0.5% organosilicon defoamer, 1% sodium sorbate, deionized water to make up the balance.

[0062] Preparation Example 3: 27% Dinotefuran·Prothioconazole·Fluoxam-aniline Seed Treatment Suspension (25% + 1% + 1%)

[0063] Formula composition: 25% fipronil, 1% prothioconazole, 1% fluopyram aniline, 3% isotridecyl alcohol polyoxyethylene ether, 3% naphthalene sulfonate formaldehyde condensate, 0.5% sodium lignosulfonate, 3% polyacrylic acid, 0.25% xanthan gum, 2% rose red pigment, 5% ethylene glycol, 0.5% magnesium aluminum silicate, 0.5% organosilicon defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0064] Preparation Example 4: 39% dinotefuran·prothiophanate-methyl·fluopyrazoline seed treatment suspension (35% + 1% + 3%)

[0065] Formula composition: 35% fipronil, 1% prothioconazole, 3% fluopyram, 5% fatty alcohol polyoxyethylene ether, 4% castor oil polyoxyethylene ether phosphate, 0.5% sodium lignosulfonate, 2% polyethylene glycol, 0.3% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0066] Preparation Example 5: 41% Imidacloprid·Prothioconazole·Fluoxam aniline Seed Treatment Suspension (35% + 1% + 5%)

[0067] Formula composition: 35% imidacloprid, 1% prothioconazole, 5% fluopyram aniline, 5% fatty alcohol polyoxyethylene ether, 4% EO / PO block copolymer, 3% phenethylphenol polyoxyethylene ether, 2% naphthalene sulfonate formaldehyde condensate, 1.5% polyethylene glycol, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 1% silicone defoamer, 1% Kathon, deionized water to make up the balance.

[0068] Preparation Example 6: 29% Imidacloprid·Prothioconazole·Fluoxam aniline Seed Treatment Suspension (25% + 1% + 3%)

[0069] Formula composition: 25% imidacloprid, 1% prothioconazole, 3% fluopyram aniline, 4% alkylphenol polyoxyethylene ether phosphate, 5% EO / PO block copolymer, 3% isotridecyl alcohol polyoxyethylene ether, 2% sodium dodecylbenzene sulfonate, 1% polyacrylic acid, 0.3% xanthan gum, 7% rose red pigment, 6% glycerol, 1% magnesium aluminum silicate, 1% silicone defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0070] Preparation Example 7: 36% Acetamiprid·Prothioconazole·Fluoropyram seed treatment suspension (25% + 1% + 10%)

[0071] Formula composition: 25% acetamiprid, 1% prothioconazole, 10% fluopyram aniline, 3% alkylphenol polyoxyethylene ether phosphate, 5% EO / PO block copolymer, 3% isotridecyl alcohol polyoxyethylene ether, 2% sodium dodecylbenzene sulfonate, 2% polyacrylic acid, 0.3% xanthan gum, 7% rose red pigment, 6% glycerol, 1% magnesium aluminum silicate, 1% silicone defoamer, 0.75% sodium benzoate, deionized water to make up the balance.

[0072] Preparation Example 8: 27% Acetamiprid·Prothioconazole·Fluoxaflutole Seed Treatment Suspension (25% + 1% + 1%)

[0073] Formula composition: 25% acetamiprid, 1% prothioconazole, 1% fluopyram aniline, 4% castor oil polyoxyethylene ether phosphate, 5% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% isotridecyl alcohol polyoxyethylene ether, 0.5% sodium lignosulfonate, 1% polyacrylic acid, 0.25% xanthan gum, 6% rose red pigment, 4% ethylene glycol, 1% magnesium aluminum silicate, 1.5% organosilicon defoamer, 0.5% potassium benzoate, deionized water to make up the balance.

[0074] Preparation Example 9: 29% Thiamethoxam·Prothioconazole·Fluoxam-aniline Seed Treatment Suspension Concentrate (25% + 1% + 3%)

[0075] Formula composition: 25% thiamethoxam, 1% prothioconazole, 3% fluopyram aniline, 2% alkylphenol polyoxyethylene ether, 4% EO / PO block copolymer, 2% alkylaryl polyoxyethylene polyoxypropylene ether, 2% sodium carboxymethyl starch, 1% sodium polycarboxylate, 0.25% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.75% silicone defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0076] Preparation Example 10: 27% Thiamethoxam·Prothioconazole·Fluoxaflutole Seed Treatment Suspension (25% + 1% + 1%)

[0077] Formula composition: 25% thiamethoxam, 1% prothioconazole, 1% fluopyram aniline, 4% glycerol fatty acid ester polyoxyethylene ether phosphate, 5% EO / PO block copolymer, 3% alkylphenol polyoxyethylene ether phosphate salt, 1% sodium dodecylbenzenesulfonate, 1.5% polyacrylic acid, 0.5% xanthan gum, 6% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 1% silicone defoamer, 1% Kathon, deionized water to make up the balance.

[0078] Indoor activity test:

[0079] Example 1: Indoor activity test for maize stalk base rot

[0080] The experiment was conducted in accordance with NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part II: Tests for Inhibition of Mycelial Growth of Pathogenic Fungi - Plate Method".

[0081] Experimental target: Fusarium graminearum.

[0082] Instruments and equipment: electronic balance (sensitivity 0.1mg), biological incubator, petri dishes, pipettes, inoculators, hole punches, calipers, clean bench, alcohol lamp, etc.

[0083] Test material preparation: Fusarium graminearum was cultured on potato dextrose agar medium for later use.

[0084] Test reagents: 97% prothioconazole technical, 95% fluopyram technical, 98% thiamethoxam technical, 98% dinotefuran technical, 98% thiamethoxam technical, 98% imidacloprid technical, 96% acetamiprid technical. All of the above reagents were provided by the Group's R&D Center.

[0085] Other reagents: acetone (analytical grade), Tween 80 (chemically pure).

[0086] Preparation of stock solutions: After dissolving the technical materials of prothioconazole and fluopyram in acetone to prepare high-concentration stock solutions, prepare a mixed solution by mixing prothioconazole and fluopyram in an appropriate ratio. Then, dilute the above single agents and mixed agents with 0.1% Tween 80 aqueous solution to obtain 5 series of mass concentrations.

[0087] Based on this, two optimal ratios of prothioconazole and fluopyram were selected, and the optimal ratio mixture was used as a fungicide combination to form a ternary compound with neonicotinoid insecticides according to different mass ratios.

[0088] Experimental replication: Four petri dishes were used for each concentration of the test reagent, with one petri dish for each replicate, for a total of four replicates. A 0.1% Tween 80 aqueous solution without the reagent was used as a blank control.

[0089] Drug preparation: Under aseptic conditions, pre-melted sterile culture medium was quantitatively added to sterile Erlenmeyer flasks according to the experimental treatment. Drug solutions were quantitatively pipetted sequentially from low to high concentration and added to the flasks, then thoroughly mixed. Equal volumes were then poured into four 9cm diameter culture media to prepare drug-containing agar plates of the corresponding concentrations. A drug-free treatment was included as a blank control. Each treatment was repeated four times.

[0090] Inoculation: Under aseptic conditions, use a sterile punch to cut a mycelial cake from the edge of the pre-cultured Fusarium graminearum. Inoculate the mycelial cake onto the center of the drug-containing plate with the mycelial side facing up. Cover with the cap and place in a constant temperature incubator at 25°C for incubation.

[0091] Investigation: The growth of pathogenic mycelia was investigated based on the colony growth in blank control culture dishes. The diameter of the colonies was measured in centimeters using calipers. The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0092] Data statistics and analysis: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated, in percentage (%). The calculation results were retained to two decimal places.

[0093] D = D1 - D2

[0094] In the formula:

[0095] D – Colony growth diameter;

[0096] D1—colony diameter;

[0097] D2 – Diameter of the mushroom cake.

[0098]

[0099] In the formula:

[0100] I – Mycelial growth inhibition rate;

[0101] D0—Correlation diameter of the blank control group;

[0102] D T — Diameter of colonies grown after chemical treatment.

[0103] The data was processed using probability value analysis. The DPS statistical analysis system was used to analyze the data and derive the regression equation and EC5. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0104] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0105] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0106]

[0107] In the formula:

[0108] ATI – Actual Measured Toxicity Index of Mixtures;

[0109] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0110] M – EC of the mixture 50The unit is milligrams per liter (mg / L).

[0111] TTI = TI A ×P A +TI B ×P B

[0112] In the formula:

[0113] TTI – Theoretical Toxicity Index of Mixtures;

[0114] TI A —A. Toxicity index of drug A;

[0115] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0116] TI B —Toxicity index of drug B;

[0117] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0118]

[0119] In the formula:

[0120] CTC – Cotoxicity Coefficient;

[0121] ATI – Actual Measured Toxicity Index of Mixtures;

[0122] TTI – Theoretical Toxicity Index of Mixtures.

[0123] The test results are shown in the table below:

[0124] Table 1. Results of indoor toxicity tests of prothioconazole, fluopyram, and their mixtures against *Corn stalk rot*.

[0125] Test reagents <![CDATA[EC 50 (mg / L)]]> Regression equation Cotoxicity coefficient (CTC) Prothioconazole (A) 3.7064 y = 1.3553x + 4.2289 - Fluopyram aniline (B) 5.3948 y = 1.3702x + 3.9970 - A:B = 1:15 4.2303 y = 1.3904x + 4.1291 123.997 A:B = 1:10 3.3184 y = 1.6028x + 4.1650 156.108 A:B = 1:6 3.1303 y = 1.6157x + 4.1993 161.811 A:B = 1:3 2.5966 y = 1.7141x + 4.2897 186.522 A:B = 1:1 2.5300 y = 1.6315x + 4.3423 173.675 A:B = 3:1 2.5113 y = 1.4843x + 4.4064 160.117 A:B = 6:1 2.8805 y = 1.4310x + 4.3425 134.694 A:B = 10:1 2.9744 y = 1.6000x + 4.2425 128.259 A:B = 15:1 3.5148 y = 1.5500x + 4.1539 107.555

[0126] Indoor test results showed that the combined use of prothioconazole and fluopyram was beneficial in improving the control effect of maize stalk rot. Both prothioconazole and fluopyram showed good control effects against Fusarium graminearum. A mass ratio of prothioconazole to fluopyram of 1:15 to 10:1 exhibited a synergistic effect, while a mass ratio of prothioconazole to fluopyram of 1:3 showed better activity and its EC50 values ​​were [not specified in the original text]. 50 The concentration was 2.5966 mg / L, and the co-toxicity coefficient was 186.522. Among them, the ratio of 1:10 to 3:1 had a significant synergistic effect on the corn stalk rot pathogen.

[0127] Table 2. Indoor toxicity test results of prothioconazole + fluopyram (1:3) mixture and its mixture with thiamethoxam against maize stalk rot.

[0128] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:3) y = 1.7141x + 4.2897 2.5966 / Thiamethoxam / ＞200 / Thiamethoxam: Prothioconazole: Fluopyram = 5:1:3 y = 1.5517x + 4.5111 2.0657 125.701 Thiamethoxam: Prothioconazole: Fluopyram = 10:1:3 y = 1.3406x + 4.5940 2.0084 129.287 Thiamethoxam: Prothioconazole: Fluopyram = 15:1:3 y = 1.3441x + 4.6142 1.9365 134.087 Thiamethoxam: Prothioconazole: Fluopyram = 20:1:3 y = 1.3337x + 4.6290 1.8973 136.858 Thiamethoxam: Prothioconazole: Fluopyram = 25:1:3 y = 1.3434x + 4.6542 1.8088 143.554 Thiamethoxam: Prothioconazole: Fluopyram = 30:1:3 y = 1.3460x + 4.6402 1.8505 140.319 Thiamethoxam: Prothioconazole: Fluopyram = 35:1:3 y = 1.3502x + 4.6221 1.9051 136.297 Thiamethoxam: Prothioconazole: Fluopyram = 40:1:3 y = 1.3617x + 4.5797 2.0355 127.566 Thiamethoxam: Prothioconazole: Fluopyram = 45:1:3 y = 1.3590x + 4.5565 2.1200 122.481

[0129] Table 3. Indoor toxicity test results of prothioconazole + fluopyram (1:1) mixture and its mixture with thiamethoxam against maize stalk rot.

[0130]

[0131]

[0132] Table 4. Indoor toxicity test results of prothioconazole + fluopyram (1:3) mixture and its mixture with fipronil against corn stalk base rot.

[0133] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:3) y = 1.7141x + 4.2897 2.5966 / Fipronil / ＞200 / Fipronil: Prothioconazole: Fluopyram = 5:1:3 y = 1.5635x + 4.4850 2.1350 121.621 Fipronil: Prothioconazole: Fluopyram = 15:1:3 y = 1.4313x + 4.5732 1.9869 130.686 Fipronil: Prothioconazole: Fluopyram = 25:1:3 y = 1.6072x + 4.5788 1.8285 142.007 Fipronil: Prothioconazole: Fluopyram = 35:1:3 y = 1.5743x + 4.4901 2.1082 123.167 Fipronil: Prothioconazole: Fluopyram = 45:1:3 y = 1.6667x + 4.3815 2.3501 110.489

[0134] Table 5. Indoor toxicity test results of prothioconazole + fluopyram (1:1) mixture and its mixture with fipronil against corn stalk base rot.

[0135] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:1) y = 1.6315x + 4.3423 2.5300 / Fipronil / ＞200 / Fipronil: Prothioconazole: Fluopyram = 5:1:1 y = 1.5478x + 4.5151 2.0572 122.983 Fipronil: Prothioconazole: Fluopyram = 15:1:1 y = 1.4283x + 4.6270 1.8247 138.653 Fipronil: Prothioconazole: Fluopyram = 25:1:1 y = 1.5734x + 4.5915 1.8181 139.156 Fipronil: Prothioconazole: Fluopyram = 35:1:1 y = 1.5840x + 4.5315 1.9758 128.049 Fipronil: Prothioconazole: Fluopyram = 45:1:1 y = 1.5720x + 4.4817 2.1367 118.407

[0136] Table 6. Indoor toxicity test results of prothioconazole + fluopyram (1:3) mixture and its mixture with imidacloprid against corn stalk rot.

[0137]

[0138]

[0139] Table 7. Indoor toxicity test results of prothioconazole + fluopyram (1:5) mixture and its mixture with imidacloprid against corn stalk rot.

[0140] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:5) y = 1.6780x + 4.2750 2.7020 / Imidacloprid / ＞200 / Fipronil: Prothioconazole: Fluopyram = 5:1:5 y = 1.4643x + 4.4444 2.3956 112.790 Fipronil: Prothioconazole: Fluopyram = 15:1:5 y = 1.7232x + 4.4184 2.1752 124.218 Fipronil: Prothioconazole: Fluopyram = 25:1:5 y = 1.4894x + 4.5716 1.9392 139.336 Fipronil: Prothioconazole: Fluopyram = 35:1:5 y = 1.3996x + 4.5818 1.9899 135.786 Fipronil: Prothioconazole: Fluopyram = 45:1:5 y = 1.4435x + 4.4870 2.2666 119.209

[0141] Table 8. Indoor toxicity test results of prothioconazole + fluopyram (1:10) mixture and its mixture with acetamiprid against maize stalk rot.

[0142]

[0143] Table 9. Indoor toxicity test results of prothioconazole + fluopyram (1:1) mixture and its mixture with acetamiprid against maize stalk rot.

[0144] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:1) y = 1.6315x + 4.3423 2.5300 / Acetamiprid / ＞200 / Acetamiprid: Prothioconazole: Fluopyram = 5:1:1 y = 1.5248x + 4.5271 2.0424 123.874 Acetamiprid: Prothioconazole: Fluopyram = 15:1:1 y = 1.5489x + 4.5485 1.9566 129.306 Acetamiprid: Prothioconazole: Fluopyram = 25:1:1 y = 1.4629x + 4.6062 1.8586 136.124 Acetamiprid: Prothioconazole: Fluopyram = 35:1:1 y = 1.5658x + 4.5522 1.9319 130.959 Acetamiprid: Prothioconazole: Fluopyram = 45:1:1 y = 1.4923x + 4.5393 2.0356 124.288

[0145] Table 10 Results of indoor toxicity tests of prothioconazole + fluopyram (1:3) mixture and its mixture with thiamethoxam against maize stalk rot.

[0146] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (1:3) y = 1.7141x + 4.2897 2.5966 / Thiamethoxam / ＞200 / Thiamethoxam: Prothioconazole: Fluopyram = 5:1:3 y = 1.4999x + 4.4840 2.2082 117.589 Thiamethoxam: Prothioconazole: Fluopyram = 15:1:3 y = 1.6093x + 4.5530 1.8956 136.980 Thiamethoxam: Prothioconazole: Fluopyram = 25:1:3 y = 1.4882x + 4.5945 1.8727 138.655 Thiamethoxam: Prothioconazole: Fluopyram = 35:1:3 y = 1.5377x + 4.5440 1.9794 131.181 Thiamethoxam: Prothioconazole: Fluopyram = 45:1:3 y = 1.4464x + 4.5211 2.1434 121.144

[0147] Table 11 Results of indoor toxicity tests of prothioconazole + fluopyram (3:1) mixture and its mixture with thiamethoxam against maize stalk rot.

[0148] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Prothioconazole: Fluopyram aniline (3:1) y = 1.4843x + 4.4064 2.5113 / Thiamethoxam / ＞200 / Thiamethoxam: Prothioconazole: Fluopyram = 5:3:1 y = 1.5122x + 4.5207 2.0746 121.050 Thiamethoxam: Prothioconazole: Fluopyram = 15:3:1 y = 1.6727x + 4.5022 1.9842 126.565 Thiamethoxam: Prothioconazole: Fluopyram = 25:3:1 y = 1.5978x + 4.5813 1.8283 137.357 Thiamethoxam: Prothioconazole: Fluopyram = 35:3:1 y = 1.5788x + 4.5685 1.8763 133.843 Thiamethoxam: Prothioconazole: Fluopyram = 45:3:1 y = 1.5552x + 4.4831 2.1496 116.826

[0149] Note: In Table 2-11, the neonicotinoid insecticides (thiamethoxam, dinotefuran, imidacloprid, acetamiprid, and thiamethoxam) showed no significant inhibitory activity against Fusarium graminearum at the designed maximum concentration of 200 mg / L.

[0150] The results in Table 2-11 show that neonicotinoid insecticides have no significant activity against Fusarium graminearum. However, when the mass ratio of prothioconazole + fludioxonil was 1:3, 1:1, and 1:5, the mixture showed a synergistic effect with neonicotinoid insecticides against Fusarium graminearum.

[0151] Example 2: Indoor activity test of corn aphids

[0152] Test basis: The test refers to NY / T 1154.6-2006 Agricultural Industry Standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticide Activity Test - Immersion Method".

[0153] Test target: maize aphid (Rhopalosiphum maidis).

[0154] Instruments and equipment: electronic balance (sensitivity 0.1mg), dissecting microscope, paintbrush, volumetric flask, beaker, pipette, petri dish, filter paper, pins, etc.

[0155] Test agents: 97% prothioconazole technical, 95% fluopyram technical, 98% thiamethoxam technical, 98% dinotefuran technical, 98% thiamethoxam technical, 98% imidacloprid technical, 96% acetamiprid technical. All of the above agents were provided by the Group's R&D Center.

[0156] Other reagents: acetone (analytical grade), Tween 80 (chemically pure).

[0157] Drug preparation: Dissolve the above raw materials in acetone first, then dilute with 0.1% Tween-80 aqueous solution. Prepare single-agent stock solutions separately, and design appropriate ratios according to the purpose of mixing and drug activity. Prepare five series of mass concentrations for each single agent and each group of mixed solutions according to the ratio method.

[0158] Experimental replication: Each concentration of the test agent was treated 4 times, with 15 healthy and uniform adult aphids per replication.

[0159] Chemical treatment: Select corn leaves, remove winged aphids, nymphs and unhealthy adult aphids with a brush, and keep 15 healthy adult aphids of the same condition. Immerse the corn leaves with adult aphids in each treatment solution for 5 seconds, then take them out and use absorbent paper to dry off the excess chemical, and place them in a petri dish lined with filter paper.

[0160] Feeding and observation: The treated test insects were placed in an incubator at 20±1℃, relative humidity of 60%~70%, and photoperiod L:D=16h:8h for cultivation.

[0161] Experimental investigation: Microscopic examination was conducted 48 hours after pesticide treatment to investigate the mortality of test insects. The standard for judging the mortality of test insects was that the adult aphids did not move when touched lightly with a pin or a brush, which was considered as death.

[0162] Data Statistics and Analysis: Based on the survey data, the corrected mortality rates for each treatment were calculated. The calculations were performed using formulas (1) and (2), and the results were rounded to two decimal places.

[0163]

[0164] In the formula:

[0165] P – Mortality rate, expressed as a percentage (%);

[0166] K – Number of dead insects, in heads;

[0167] N – Total number of insects treated, in heads.

[0168]

[0169] In the formula:

[0170] P1—Adjusted mortality rate, in percentage (%);

[0171] P t —The mortality rate is expressed as a percentage (%).

[0172] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0173] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is >20%, the experiment needs to be repeated.

[0174] The data was processed using probability value analysis. The DPS statistical analysis system was used to derive the regression equation and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0175] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0176] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formulas (3), (4), and (5):

[0177]

[0178] In the formula:

[0179] ATI – Actual Measured Toxicity Index of Mixtures;

[0180] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0181] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0182] TTI = TI A ×P A +TI B ×P B ··········(4)

[0183] In the formula:

[0184] TTI – Theoretical Toxicity Index of Mixtures;

[0185] TI A —A. Toxicity index of drug A;

[0186] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0187] TI B —Toxicity index of drug B;

[0188] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0189]

[0190] In the formula:

[0191] CTC – Cotoxicity Coefficient;

[0192] ATI – Actual Measured Toxicity Index of Mixtures;

[0193] TTI – Theoretical Toxicity Index of Mixtures.

[0194] The test results are shown in the table below:

[0195] Table 12 Results of indoor toxicity tests on corn aphids using a mixture of prothiobacillus and fluopyram with thiamethoxam.

[0196] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Thiamethoxam y = 1.2904x + 4.1037 4.9449 - Prothioconazole: Fluopyram = 1:3 - ＞200 - Prothioconazole: Fluopyram aniline = 1:1 - ＞200 - Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 5:1:3 y = 1.4352x + 4.1611 3.8419 128.710 Thiamethoxam: Prothioconazole-fluoxazole-aniline = 10:1:3 y = 1.3057x + 4.2678 3.6373 135.950 Thiamethoxam: Prothioconazole-fluoxazole-aniline = 15:1:3 y = 1.2708x + 4.3096 3.4935 141.546 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 25:1:3 y = 1.4153x + 4.2846 3.2024 154.412 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 35:1:3 y = 1.3444x + 4.2561 3.5753 138.307 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 45:1:3 y = 1.3583x + 4.1971 3.9004 126.779 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 5:1:1 y = 1.3274x + 4.2443 3.7095 133.304 Thiamethoxam: Prothioconazole-fluoxazole-aniline = 10:1:1 y = 1.3293x + 4.2743 3.5149 140.684 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 15:1:1 y = 1.3095x + 4.3064 3.3856 146.057 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 25:1:1 y = 1.3709x + 4.2800 3.3514 147.547 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 35:1:1 y = 1.3602x + 4.2561 3.5229 140.364 Thiamethoxam: Prothioconazole, Fluopyram, and Aniline = 45:1:1 y = 1.2689x + 4.2604 3.8270 129.211

[0197] Table 13 Results of indoor toxicity tests on corn aphids using a mixture of prothiobacillus and fluopyram with aniline and dinotefuran.

[0198] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Fipronil y = 1.4645x + 4.0705 4.3120 - Prothioconazole: Fluopyram = 1:3 - ＞200 - Prothioconazole: Fluopyram aniline = 1:1 - ＞200 - Fipronil: Prothioconazole: Fluopyram = 10:1:3 y = 1.3871x + 4.2800 3.3045 130.489 Fipronil: Prothioconazole: Fluopyram = 25:1:3 y = 1.3619x + 4.3648 2.9268 147.328 Fipronil: Prothioconazole: Fluopyram = 35:1:3 y = 1.6754x + 4.1710 3.1246 138.002 Fipronil: Prothioconazole: Fluopyram = 10:1:1 y = 1.3818x + 4.2326 3.5918 120.051 Fipronil: Prothioconazole: Fluopyram = 25:1:1 y = 1.3515x + 4.3206 3.1823 135.499 Fipronil: Prothioconazole: Fluopyram = 351:1 y = 1.5661x + 4.1587 3.4448 125.174

[0199] Table 14 Results of indoor toxicity tests on corn aphids using a mixture of prothiophanate-methyl and fluopyram with imidacloprid.

[0200] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Imidacloprid y = 1.3476x + 4.4416 2.5966 - Prothioconazole: Fluopyram = 1:3 - ＞200 - Prothioconazole: Fluopyram aniline = 1:5 - ＞200 - Imidacloprid: Prothioconazole: Fluopyram = 10:1:3 y = 1.3076x + 4.5843 2.0792 124.885 Imidacloprid: Prothioconazole: Fluopyram = 25:1:3 y = 1.2538x + 4.6495 1.9036 136.405 Imidacloprid: Prothioconazole: Fluopyram = 35:1:3 y = 1.3504x + 4.5768 2.0578 126.183 Imidacloprid: Prothioconazole: Fluopyram = 10:1:5 y = 1.3969x + 4.5393 2.1369 121.512 Imidacloprid: Prothioconazole: Fluopyram = 25:1:5 y = 1.3033x + 4.6265 1.9347 134.212 Imidacloprid: Prothioconazole: Fluopyram = 35:1:5 y = 1.4125x + 4.6245 1.8444 140.783

[0201] Table 15 Results of indoor toxicity tests on corn aphids using a mixture of prothiobacillus and fluopyram with acetamiprid.

[0202] Test reagents Regression equation <![CDATA[EC 50 (mg / L)]]> Cotoxicity coefficient Acetamiprid y = 1.3507x + 4.3348 3.1080 - Prothioconazole: Fluopyram aniline = 1:10 - ＞200 - Prothioconazole: Fluopyram aniline = 1:1 - ＞200 - Acetamiprid: Prothioconazole: Fluopyram aniline = 10:1:10 y = 1.2388x + 4.4775 2.6409 117.687 Acetamiprid: Prothioconazole: Fluopyram = 25:1:10 y = 1.2999x + 4.4806 2.5094 123.854 Acetamiprid: Prothioconazole: Fluopyram = 35:1:10 y = 1.3064x + 4.4270 2.7453 113.212 Acetamiprid: Prothioconazole: Fluopyram aniline = 10:1:1 y = 1.3031x + 4.4987 2.4251 128.160 Acetamiprid: Prothioconazole: Fluopyram = 25:1:1 y = 1.3438x + 4.5000 2.3557 131.935 Acetamiprid: Prothioconazole: Fluopyram = 35:1:1 y = 1.3257x + 4.4721 2.5017 124.236

[0203] Table 16 Results of indoor toxicity tests on corn aphids using a mixture of prothiobacillus and fluopyram with thiamethoxam.

[0204]

[0205]

[0206] Note: In Table 12-16, the ratios of prothioconazole and fluopyram at 1:3 and 1:1 showed no significant activity against corn aphids at the designed maximum concentration of 200 mg / L.

[0207] Table 12-16, based on the results of indoor activity testing, shows that neonicotinoid insecticides have a good control effect on corn aphids. The experimental results also indicate that combining a mixture of prothioconazole and fluopyram with neonicotinoid insecticides to form a ternary compound formulation significantly increases the toxicity of the neonicotinoid insecticides and enhances their control efficacy against pests.

[0208] Field efficacy trials:

[0209] Example 3: Field efficacy trial of pesticides for maize stalk base rot

[0210] Experimental location: Corn fields in Xixia County, Nanyang City, Henan Province. All experimental plots had the same cultivation conditions and good irrigation conditions. The previous crop was wheat.

[0211] Experimental target: Corn stalk rot.

[0212] Experimental crop: maize (Yinhai 568).

[0213] Test reagents: The test reagents and dosages are shown in Table 8.

[0214] Table 17 Test reagents and dosages

[0215] serial number Drug Name Dosage of active ingredient (g / 100kg seeds) 1 32% Thiamethoxam·Prothioconazole·Fluoxam-aniline Seed Treatment Suspension Concentrate (30%+1%+1%) 100 2 27% Dinotefuran·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 1%) 100 3 41% Imidacloprid·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (35% + 1% + 5%) 100 4 29% Thiamethoxam·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 3%) 100 5 36% Acetamiprid·Prothioconazole·Fluoxam aniline Seed Treatment Suspension Concentrate (25% + 1% + 10%) 100 6 13% Thiamethoxam·Thifluzamide·Tebuconazole Seed Treatment Suspension 130 7 10% Thiamethoxam·Fluordioxonil·Methoxyfen Seed Treatment Suspension Concentrate 100 8 35% Pyraclostrobin·Fluordioxonil·Thiamethoxam Seed Treatment Suspension Concentrate 210 9 Water comparison -

[0216] Experimental Design: The experiment adopted a randomized block design, with each plot having an area of ​​40m². 2 The experiment was conducted on June 7, 2019, with water-treated seeds serving as a control. Each treatment was repeated four times.

[0217] Experimental treatment: One day before corn sowing, accurately weigh the amount of pesticide solution according to the amount of seeds used in the plot, pour it into the seed dressing bottle and mix the seeds so that the pesticide solution is evenly attached to the surface of the seeds. After drying, it is ready for use.

[0218] Survey Methods: Five points were randomly selected from each plot, with 20 corn plants marked at each point, for a total of 100 plants per plot. The emergence status was tested after seedling emergence, and the number of seedlings was recorded and the emergence rate was calculated. During the seedling stage, the incidence of corn stalk rot was investigated, the number of infected plants was recorded, and the control effect was calculated.

[0219] Methods for calculating drug efficacy:

[0220]

[0221]

[0222] Experimental results:

[0223] Table 18 Results of field efficacy trials for maize stalk base rot.

[0224]

[0225] As shown in Table 18, the experimental results indicate that when prothioconazole is mixed with fluopyram and neonicotinoid insecticides (such as thiamethoxam, dinotefuran, imidacloprid, acetamiprid, or thiamethoxam), and the effective ingredient dosage is 100g / 100kg of seeds, the average disease incidence rate of each preparation treatment is 3.50%–5.25%, and the control efficacy against maize stalk rot is 85.91%–90.60%, demonstrating good control effects. Furthermore, the germination rate of each preparation treatment group is significantly higher than that of other treatment groups, indicating that the pesticide composition of the present invention does not cause phytotoxicity to seeds and can control soil-borne diseases and underground pests.

[0226] Example 4: Field efficacy trial against corn aphids

[0227] Experimental site: Cornfield in Huguan County, Changzhi City, Shanxi Province. The soil fertility is moderate, the terrain is flat, the organic matter content is 1.9%, the pH value is 7.0, and the occurrence of corn aphids has been severe in recent years.

[0228] Experimental target: corn aphid.

[0229] Experimental crop: Maize (Jindan 39).

[0230] Test reagents: The test reagents and dosages are shown in Table 19.

[0231] Table 19 Test reagents and dosages

[0232] serial number Drug Name Dosage of active ingredient (g / 100kg seeds) 1 32% Thiamethoxam·Prothioconazole·Fluoxam-aniline Seed Treatment Suspension Concentrate (30%+1%+1%) 100 2 27% Dinotefuran·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 1%) 100 3 41% Imidacloprid·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (35% + 1% + 5%) 100 4 29% Thiamethoxam·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 3%) 100 5 36% Acetamiprid·Prothioconazole·Fluoxam aniline Seed Treatment Suspension Concentrate (25% + 1% + 10%) 100 6 13% Thiamethoxam·Thifluzamide·Tebuconazole Seed Treatment Suspension 130 7 10% Thiamethoxam·Fluordioxonil·Methoxyfen Seed Treatment Suspension Concentrate 100 8 35% Pyraclostrobin·Fluordioxonil·Thiamethoxam Seed Treatment Suspension Concentrate 210 9 Clear water blank control -

[0233] Experimental design: The experimental plots were arranged in a randomized block design, with each plot having an area of ​​20m². 2 Each treatment was repeated four times. The test agent was applied to the seeds according to the experimental design, with water-treated seeds serving as a control. Before sowing, the required dosage of each agent and the required amount of seeds were weighed out, poured into a seed-mixing bottle, mixed evenly, and then dried for later use.

[0234] Crop safety survey: After emergence, the growth of maize in each treatment was observed periodically by visual inspection.

[0235] Survey Methods: The experiment was conducted during the initial peak period of corn aphid infestation. Five sites were randomly selected for each treatment, with two plants surveyed at each site. The entire plant was surveyed, and the number of wingless aphids was counted to calculate the control effect.

[0236] Method for calculating the effectiveness of prevention:

[0237]

[0238] The field efficacy trials are shown in the table below:

[0239] Table 20 Results of field efficacy trials for corn aphids

[0240] serial number Drug Name Preventive efficacy (%) Growth status 1 41% Imidacloprid·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (35% + 1% + 5%) 92.77 normal 2 27% Dinotefuran·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 1%) 91.63 normal 3 36% Acetamiprid·Prothioconazole·Fluoxam aniline Seed Treatment Suspension Concentrate (25% + 1% + 10%) 89.43 normal 4 29% Thiamethoxam·Prothioconazole·Fluoxam Aniline Seed Treatment Suspension Concentrate (25% + 1% + 3%) 94.04 normal 5 32% Thiamethoxam·Prothioconazole·Fluoxam-aniline Seed Treatment Suspension Concentrate (30%+1%+1%) 93.69 normal 6 13% Thiamethoxam·Thifluzamide·Tebuconazole Seed Treatment Suspension 81.91 normal 7 10% Thiamethoxam·Fluordioxonil·Methoxyfen Seed Treatment Suspension Concentrate 80.64 normal 10 35% Pyraclostrobin·Fluordioxonil·Thiamethoxam Seed Treatment Suspension Concentrate 84.82 normal 21 Clear water blank control - normal

[0241] Field efficacy trials have shown that the pesticide composition of this invention has a good control effect on corn aphids and is safe for corn plants.

[0242] Through indoor toxicity testing and field efficacy trials, the combined seed treatment with the prothioconazole, fluopyram, and neonicotinoid insecticides (any one of thiamethoxam, dinotefuran, imidacloprid, acetamiprid, or thiamethoxam) described in this invention showed good control effects against crop diseases and pests. The pesticide composition or formulation obtained by this invention exhibits significant efficacy and can delay the development of pesticide resistance in pathogens and pests. Furthermore, no phytotoxicity was observed in the experiments, indicating that the improved synergistic effect of the resulting pesticide composition or formulation in both fungicide and insecticide control can reduce production and usage costs while ensuring crop safety.

[0243] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that, The pesticide composition comprises active ingredient A, active ingredient B, and active ingredient C. Active ingredient A is prothioconazole, active ingredient B is fluopyram, and active ingredient C is a neonicotinoid insecticide. The neonicotinoid insecticide is any one of clothianidin, dinotefuran, imidacloprid, acetamiprid, or thiacloprid. The mass ratio of active ingredient A to active ingredient B is 1:15 to 10:

1. The mass ratio of the total mass of active ingredients A and B to the mass of active ingredient C is 2:45 to 16:

5.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:10 to 6:

1.

3. The pesticide composition according to claim 1, characterized in that, The total mass ratio of active ingredient A and active ingredient B to the mass ratio of active ingredient C is 2:35 to 16:

5.

4. The pesticide composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the pesticide composition, the total weight of active ingredient A, active ingredient B and active ingredient C accounts for 0.5% to 80% of the total content of the pesticide composition.

5. The pesticide composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the pesticide composition, the total weight of active ingredient A, active ingredient B and active ingredient C accounts for 1% to 70% of the total content of the pesticide composition.

6. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition also includes auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, warning colors, film-forming agents, synergists, and carriers.

7. The pesticide composition according to claim 1, characterized in that, The pesticide composition is prepared into an agriculturally permissible formulation, wherein the formulation is any one of seed treatment dry powder, seed treatment dispersible powder, seed treatment liquid, seed treatment emulsion, and / or seed treatment suspension.

8. The pesticide composition according to claim 7, characterized in that, The formulation is a seed treatment suspension.

9. The use of the pesticide composition according to any one of claims 1-8 for the control of plant diseases and / or pests, characterized in that, The plant in question is corn, the disease is corn stalk rot, and the pest is corn aphid.

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