A kind of factory-based cultivation method of Pleurotus geesteranus

Abstract

The invention discloses a method for cultivating pleurotus geesteranus in a factory, which comprises the following steps: S1: preparation of pleurotus geesteranus liquid strains; S2: preparation of pleurotus geesteranus liquid production strains; S3: preparation and sterilization of fungus bags; S4: inoculation and cultivation; S5: mushroom production management; The technical core points of the invention are: First, the invention has made comprehensive improvements and designs from the links of fungus production, inoculation and mushroom production management; Second, the inoculation and cultivation methods are innovated; Third, the environmental parameters of the fungus bag cultivation are innovated, and the constant temperature cultivation is transformed into variable temperature cultivation; Fourth, the mushroom production method is innovated, and the factory-based bottle cultivation unidirectional mushroom production is changed into the bag cultivation bidirectional mushroom production mode; The time for the factory-based bag cultivation fungus bag to be full of fungus is shortened to 20 to 22 days, which is shortened by 35.7% to 52.1% compared with the solid inoculation and constant temperature cultivation methods. The quality of the fungus bag is significantly improved, the mycelium is dense, and the fungus bag has the same age; Due to the fast mushroom production speed and vigorous growth, the finished product rate ratio of the fungus bag is increased by more than 4.6 percentage points compared with the solid inoculation and constant temperature cultivation methods.

Description

Technical Field

[0001] The invention relates to the technical field of edible mushroom cultivation, in particular to a factory-based Pleurotus geesteranus cultivation method. Background Art

[0002] Pleurotus pulmonarius belongs to Fungi, Basidiomycetes, Agaricales, Pleurotus genus, Pleurotus geesteranus family. Pleurotus geesteranus is a temperature-dependent mushroom species. It can produce mushrooms in batches in a row after low temperature stimulation in summer. Therefore, the timing of fruiting can be artificially controlled, and it has good characteristics for large-scale and commercial production.

[0003] At present, the domestic production of Pleurotus geesteranus is mainly carried out in greenhouse bags in summer. The fruiting period starts in May and ends in October each year. The cultivation cycle (from inoculation to harvesting) takes about 6 to 7 months. Due to the long time span and the 3 to 4 months of high temperature season, the mushroom bags are very susceptible to damage from high temperature, diseases and insect pests, which will cause economic losses to the production entities. Therefore, the factory production model of Pleurotus geesteranus has gradually developed in recent years.

[0004] However, most of the current factory-based cultivation of Pleurotus geesteranus imitates the production model of other mushroom species, especially Flammulina velutipes, that is, the bottle cultivation model, and bottle-grown edible mushrooms are only harvested once from the bottle mouth. The fruiting characteristics of Pleurotus geesteranus determine that if only one wave of mushrooms is harvested from the bottle mouth, its yield will be seriously affected. Harvesting multiple waves of mushrooms will not reflect the advantages of factory-based efficient cultivation, and energy consumption will also increase significantly. Therefore, solving the contradiction between yield and cultivation efficiency is the key to factory-based cultivation of Pleurotus geesteranus.

[0005] The reference "Factory-based Bottle Cultivation Technology of Pleurotus geesteranus" (Mao Xiaowei, Wu Youliang, Chen Xiaoping, Factory-based Bottle Cultivation Technology of Pleurotus geesteranus, "China Agricultural Technology Extension", Issue 7, 2021, 42-43) only introduces the production model of Pleurotus geesteranus, which is one-way fruiting at the bottle mouth. If the multi-wave fruiting management model is still adopted, it does not conform to the trend of factory-based efficient cultivation. If only one wave is harvested, the yield will be significantly reduced, which does not conform to the high-yield and efficient production management model. The invention patent with application number CN202110751788.X discloses "A factory-based Pleurotus geesteranus cultivation process by improving the substrate and planting conditions", which actually changes the cultivation substrate formula and planting environment parameters, while the fruiting basically follows the traditional summer cold fruiting method. The "A method for cultivating Pleurotus geesteranus in factories" with application number CN202010901642.4 cultivates Pleurotus geesteranus in factories through mycelium cultivation, standardized bud induction, mushroom production management, and tide-changing period management, but it still adopts a multi-tide management method, especially the time for cultivating mushrooms increases by 5 days for each additional tide, which does not meet the requirements of efficient factory cultivation (high yield and short cycle). Therefore, the above three methods do not solve the contradiction between high yield and efficient cultivation of Pleurotus geesteranus. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention integrates and innovates a factory-based method for efficiently producing mushrooms from bagged cultivation of Pleurotus geesteranus, taking both yield and cultivation efficiency into consideration, thereby achieving efficient cultivation of Pleurotus geesteranus.

[0007] The above technical objectives of the present invention are achieved through the following technical solutions:

[0008] A method for cultivating Pleurotus geesteranus in an industrial scale, characterized in that the method comprises the following steps:

[0009] S1: Preparation of liquid spawn of Pleurotus geesteranus

[0010] S101: preparing mother culture medium;

[0011] S102: inoculating the mother culture medium with Pleurotus geesteranus test tube cultures;

[0012] Prepare a mother strain of liquid spawn of Pleurotus geesteranus, and then use the mother strain of liquid spawn of Pleurotus geesteranus to amplify and culture to prepare a liquid production strain of Pleurotus geesteranus;

[0013] S2: preparing liquid production seed of Pleurotus geesteranus; preparing and sterilizing a mushroom bag, wherein the center of the mushroom bag has a reserved hole, and injecting the liquid production seed of Pleurotus geesteranus prepared in step (1) into the mushroom bag through the reserved hole with an inoculation needle to complete the inoculation;

[0014] S3: Preparation and sterilization of fungus bags;

[0015] S4: inoculation and cultivation;

[0016] S5: Mushroom production management.

[0017] As a further optimization scheme of the present invention, the step S101 of configuring the mother culture medium specifically includes: using PDA liquid culture medium, taking fresh potatoes, peeling them, cutting them into strips or slices, adding water and boiling them until the potatoes are cooked but not mushy, filtering them with gauze, adding glucose to the filtrate, fixing the volume, packaging and sterilizing them, and cooling them for later use.

[0018] As a further optimization scheme of the present invention, the step S102 of inoculating Pleurotus geesteranus test tube seeds in the mother seed culture medium specifically includes: taking the vigorously growing Pleurotus geesteranus test tube seeds, inoculating them into the above-mentioned mother seed culture medium, and culturing them on a shaking table, so that the Pleurotus geesteranus liquid spawn mother seed is prepared.

[0019] As a further optimization scheme of the present invention, the step S2 of preparing the liquid production seed of Pleurotus geesteranus specifically comprises:

[0020] S201: Prepare liquid culture medium: select a liquid production formula and add water to 750L to 800L;

[0021] S202: The above formula is loaded into a fermentation tank for fermentation.

[0022] As a further optimization scheme of the present invention, the liquid production formula in step S201 includes, by weight: 20kg-25kg of white sucrose, 5kg-10kg of glucose, 5kg-7.5kg of soybean powder, 4kg-5kg of corn powder, 4kg-5kg of bran, 5kg of yeast extract, 0.5kg-1kg of potassium dihydrogen phosphate, 0.5kg-1kg of magnesium sulfate, and 0.5kg of defoaming agent.

[0023] As a further optimization scheme of the present invention, the fermentation in the fermentation tank in step S202 specifically includes: loading the liquid culture medium into the fermentation tank in proportion, culturing and sterilizing, and after the sterilization is completed, waiting for the temperature to drop to 20° C., connecting the Pleurotus geesteranus liquid spawn to the fermentation tank in a sterile room, maintaining the fermentation tank inlet pressure of 0.15 MPa, the air in the fermentation tank is sterile, and the liquid culture medium is continuously rolled.

[0024] As a further optimization scheme of the present invention, the step S3 of making and sterilizing fungus bags specifically includes:

[0025] S301: Equipped with mushroom bag cultivation formula;

[0026] S302: Prepare mushroom bags: stir the above formula to make its water content reach 63-65%, reserve a hole in the center of the mushroom bag after bagging, the hole diameter is 2cm, the hole depth is 17-18cm, and high pressure sterilization is performed after bagging.

[0027] As a further optimization scheme of the present invention, the bag planting formula in step S301 includes, by mass fraction, 45% of miscellaneous sawdust, 25% of cottonseed hulls, 10% of corn cobs, 18% of bran, and 2% of lime; 35% of miscellaneous sawdust, 15% of mulberry branch chips, 20% of cottonseed hulls, 12% of corn cobs, 16% of bran, and 2% of lime.

[0028] As a further optimization scheme of the present invention, the step S4 of inoculation and culturing specifically includes: injecting the liquid spawn of Pleurotus geesteranus into the sterilized spawn bag from the reserved hole with an inoculation needle, setting the temperature of the culture room to 26°C for the first 3 to 5 days after inoculation, and gradually lowering the temperature of the culture room to 23°C after 5 days when the mycelium has been colonized and germinated, and controlling the CO2 concentration in the culture room to below 0.02%, and continuously ventilating during the spawning period to ensure that the culture room has sufficient oxygen.

[0029] As a further optimization scheme of the present invention, the step S5 of mushroom production management specifically includes:

[0030] S501: Move the mushroom bags that have been fermented into the factory-made mushroom warehouse and place them on their sides on the grid racks. The temperature in the warehouse is lowered to 5-8°C. At the same time, cut the ends of the mushroom bags lying on their sides to expose the material surface.

[0031] S502: The temperature of the warehouse is raised to 28±2℃, the CO2 concentration in the warehouse is increased to 0.2%, ventilation is carried out for 5 to 10 minutes per hour, and after 70 to 72 hours, the primordium is formed;

[0032] S503: After the primordium is formed, 300 lux to 400 lux light stimulation is given to promote primordium differentiation;

[0033] S504: When the stipes of the small mushroom buds of Pleurotus geesteranus are 1 to 2 cm long, the CO2 concentration in the warehouse is reduced to 0.05% to 0.1%; when the stipes grow to 3 to 4 cm and the cap diameter reaches 2 cm, increase the ventilation volume to reduce the CO2 concentration in the warehouse to below 0.05%, maintain the light intensity at 450lux to 500lux, and control the warehouse temperature at 24±1℃ to promote the formation of high-quality fruiting bodies.

[0034] In summary, the present invention mainly has the following beneficial effects:

[0035] First, the present invention has made comprehensive improvements and designs in the aspects of strain production, inoculation and mushroom production management. The specific effect analysis is as follows: the factory-based bottle cultivation is converted to factory-based bag cultivation, and the mushroom bag production process is changed during the production of the material bag. The traditional material bag production uses a punching rod (diameter 2cm) installed on the bagging machine to punch a hole in the middle of the formed material bag, and the punching depth is about 8cm, while the actual bag material height is 20cm. Therefore, the lower half of the material bag is solid and there is no reserved hole. After solid inoculation, the mushroom bag takes 45±3 days to germinate. Due to the long germinate time, the mycelium age of the upper and lower parts of the mushroom bag is inconsistent, and the upper part of the mycelium is prone to aging to form primordia. This method is only suitable for the traditional greenhouse multi-tide mushroom production mode, and the production cycle is 6 to 7 months, while the production cycle of the present application is shortened to about 1 month to 1 and a half months.

[0036] Second, innovate the inoculation and cultivation methods. The specific effect analysis is as follows: Use liquid strain inoculation, fully insert the inoculation needle into the reserved hole, so that the inside of the fungus bag is connected to the liquid strain from top to bottom, increase the mycelium growth points, and thus significantly improve the speed of fungus germination. It only takes 25±2 days for the mycelium to fill the bag, which is more than 20 days shorter than the traditional method, and is more in line with the requirements of factory production. At the same time, because the liquid strain is evenly distributed in the reserved holes of the material bag, the mycelium growth points can germinate and grow at the same time, and the age of the entire fungus bag is guaranteed to be consistent.

[0037] Third, the parameters of the culture environment of the mushroom bag are innovated, and the constant temperature culture is transformed into variable temperature culture. The specific effect analysis is as follows: the traditional temperature of the culture environment of the Pleurotus geesteranus mushroom bag is maintained between 26℃ and 28℃, while the present invention uses liquid spawn inoculation to make the mycelium growth points spread all over the mycelium bag. After the mycelium is planted, the growth rate is fast and the oxygen demand is high. Therefore, the temperature of the culture room is set to 26℃ in the first 3 to 5 days after inoculation. After the mycelium is planted and germinated, due to the large number of growth points, the mycelium metabolism is vigorous, the respiration is enhanced, and the heat generation of the mycelium bag itself is faster than the solid inoculation method. Therefore, after the mycelium is cultured at 26℃ for about 5 days, the temperature of the culture room is gradually adjusted to 23℃ to prevent the high temperature from inhibiting the growth of mycelium or causing the phenomenon of "burning bacteria". At the same time, the CO2 concentration in the culture room is controlled below 0.02%, and continuous ventilation is carried out during the spawning period to ensure that there is sufficient oxygen in the culture room.

[0038] Fourth, the fruiting method was innovated, changing from the one-way fruiting of factory-grown bottles to the two-way fruiting mode of bag-grown plants. The specific effect analysis is as follows: Due to the use of liquid strains and deep inoculation of mushroom bags, the age of the upper and lower mushroom bags is consistent, which lays the foundation for the two-way fruiting of factory-grown bags. Under the conditions of the same formula and the same variety, the yield of one wave of mushrooms is doubled. Therefore, through this method, the mushroom bag can adopt the two-way fruiting mode when the first wave of mushrooms is produced, thereby achieving high yield and high efficiency.

[0039] At the same time, combining the first, second and third points, the time for the factory-based bag-cultivated fungus bags to fill the bag is shortened to 20 to 22 days, which is 35.7% to 52.1% shorter than the solid inoculation and constant temperature culture method. The quality of the fungus bags is significantly improved, the mycelium is dense, and the age of the fungus bags is consistent; due to the fast growth speed and vigorous growth, the finished product rate of the fungus bags is more than 4.6 percentage points higher than that of the solid inoculation and constant temperature culture method. DETAILED DESCRIPTION

[0040] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] The technical solution of the present invention is as follows:

[0042] 1. Preparation of liquid spawn of Pleurotus geesteranus

[0043] Preparation of liquid spawn of Pleurotus geesteranus:

[0044] Preparation of mother culture medium: Use PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose to the filtrate, make up to 1000ml, divide into packages, sterilize at 121℃ for 25min, and set aside after cooling.

[0045] Inoculation: Take 2-3 g of the vigorously growing Pleurotus geesteranus test tube culture medium, inoculate it into the above-mentioned mother culture medium, and culture it on a shaker (120 r / min, 26° C.). After 6-7 days, the Pleurotus geesteranus liquid spawn mother culture is ready.

[0046] 2. Production of liquid production of Pleurotus geesteranus

[0047] The formula of liquid production seed is: 20kg-25kg white cane sugar, 5kg-10kg glucose, 5kg-7.5kg bean powder, 4kg-5kg corn powder, 4kg-5kg bran, 5kg yeast paste, 0.5kg-1kg potassium dihydrogen phosphate, 0.5kg-1kg magnesium sulfate, 0.5kg defoamer, add water to 750L-800L. The production seed liquid culture medium is loaded into the fermentation tank according to the proportion, and the fermentation tank with a volume of 1000L is loaded with 750L-800L of liquid culture medium. The culture medium is sterilized at 121℃ for 30-35min. After the sterilization, wait until the culture medium temperature drops to 20℃, connect the liquid seed of Pleurotus geesteranus to the fermentation tank in the sterile room, maintain the fermentation tank air intake pressure of 0.15MPa, and ensure that the air entering the tank is sterile, so that the liquid culture medium keeps rolling. The ambient temperature of the culture room is controlled at 18℃~20℃, the air in the culture room is filtered through a 100,000-grade filter and maintained at positive pressure. After 8~10 days of cultivation, the strains can be used for inoculation of fungus bags.

[0048] 3. Production and sterilization of fungus bags

[0049] Formula for factory-based bag cultivation of Pleurotus geesteranus: 45% sawdust, 25% cottonseed hulls, 10% corn cobs, 18% bran, 2% lime; 35% sawdust, 15% mulberry branch chips, 20% cottonseed hulls, 12% corn cobs, 16% bran, 2% lime. Mix all kinds of raw materials according to the formula ratio, and the water content reaches 63-65%. The bagging machine is used for bagging, the total height of the bag is 20cm, the diameter of the reserved hole in the center of the bag is 2cm, and the hole depth is 17-18cm. After bagging, high-pressure sterilization (121℃, 2.5h) is carried out to avoid squeezing and deformation of the bag.

[0050] 4. Inoculation and cultivation

[0051] The liquid spawn of Pleurotus geesteranus was injected into the sterilized spawn bag from the reserved hole with an inoculation needle. The temperature of the culture room was set to 26°C for the first 3 to 5 days after inoculation. After 5 days, the mycelium had been colonized and germinated. The temperature of the culture room was gradually lowered to 23°C. At the same time, the CO2 concentration in the culture room was controlled below 0.02%. During the spawning period, ventilation was continued to ensure that there was sufficient oxygen in the culture room.

[0052] 5. Mushroom production management

[0053] Move the mushroom bags that have been fermented into the factory-made mushroom warehouse and place them on the grid rack on their sides. Lower the temperature in the warehouse to 5-8℃ and keep it for 10h-12h. Since the mycelium age at both ends of the mushroom bags is the same, cut the two ends of the side-lying mushroom bags to expose the material surface at the same time, spray water on the material surface at both ends once, increase the humidity in the warehouse, maintain it above 95%, and ensure that the material surface of the mushroom bags is moist after cutting; raise the temperature in the warehouse to 28±2℃; increase the CO2 concentration in the warehouse to 0.2%, and ventilate for 5-10min per hour; keep the warehouse dark, and primordium will be formed after 70-72h. After the primordium is formed, 300lux~400lux light stimulation is given to promote primordium differentiation; when the stipe of the small mushroom bud of Pleurotus geesteranus is 1~2cm, ventilation is carried out to reduce the CO2 concentration in the warehouse to 0.05%~0.1%; when the stipe grows to 3~4cm and the cap diameter reaches about 2cm, the ventilation volume is increased to reduce the CO2 concentration in the warehouse to below 0.05%, the light intensity is maintained at 450lux~500lux, and the warehouse temperature is controlled at 24±1℃ to promote the formation of high-quality fruiting bodies (it takes 60h~72h from primordium formation to harvesting). The yield of one wave of mushrooms can reach more than twice that of factory-scale bottle cultivation, achieving high efficiency and high yield.

[0054] Comparative Example 1

[0055] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0056] Mushroom production method 1: Steel frame greenhouse, low temperature in summer stimulates mushroom production.

[0057] 1. Preparation of solid spawn of Pleurotus geesteranus

[0058] Preparation of mother culture: PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose and 13g of agar powder to the filtrate, make up to 1000ml, divide into test tubes, sterilize at 121℃ for 25min, then arrange the test tubes into slant culture medium and cool for use.

[0059] Inoculation: Take a 0.5cm×0.5cm block of Pleurotus geesteranus culture and inoculate it into the above slant culture medium. Culture it at 26℃ for 10 days. When the mycelium covers the entire slant, it is the mother culture of Pleurotus geesteranus.

[0060] Original seed production:

[0061] The original culture matrix is ​​configured as follows: 85% cottonseed hulls, 12% bran, 1% sugar, and 2% lime. First, weigh the raw materials and auxiliary materials such as cotton hulls, bran, and lime according to the mass ratio, mix them, and add water while stirring to ensure sufficient stirring, so that the water content of the culture material reaches 63%. After the preparation is completed, immediately bag (bottle) and sterilize, sterilize by high pressure for 2 hours, and cool to 26°C. Then, inoculate the mother species of Pleurotus geesteranus in the clean bench, inoculation box, or inoculation room. After inoculation, move it to the culture room for cultivation. The culture temperature should be 26°C. The culture room should be ventilated twice a day for 30 minutes each time, and the relative humidity of the air should be maintained at 70%. When the mycelium fills the bottle or bag, it is the original species of Pleurotus geesteranus.

[0062] Cultivated species production:

[0063] Cultivation culture medium configuration: 40% cottonseed hulls, 40% sawdust, 18% bran, 2% lime. First weigh the raw and auxiliary materials such as cotton hulls, sawdust, bran, lime, etc. according to the mass ratio, mix them and add water while stirring, ensure sufficient stirring, and make the water content of the culture material reach 63%. After the preparation is completed, immediately bag and sterilize, autoclave for 2 hours, and cool to 26℃, and then connect the original species of Pleurotus geesteranus to the fungus bag in the clean bench, inoculation box or inoculation room. After inoculation, move it to the culture room for cultivation. The culture temperature is preferably 26℃. The culture room is twice a day, 30 minutes each time, and the relative humidity of the air is maintained at 70%. When the mycelium fills the fungus bag, it is the cultivation species of Pleurotus geesteranus.

[0064] 2. Production of Pleurotus geesteranus mushroom bags

[0065] The formula for cultivating Pleurotus geesteranus in a steel frame greenhouse is 45% sawdust, 25% cottonseed hulls, 10% corn cobs, 18% bran, and 2% lime by mass ratio. First, weigh the raw and auxiliary materials such as cotton hulls, sawdust, bran, corn flour, gypsum, lime, etc. by mass ratio, mix them, and add water while stirring to ensure sufficient stirring so that the moisture content of the culture material reaches 65%. After the preparation is completed, immediately bag and sterilize, sterilize by high pressure for 2 hours, and when it cools to 26°C, the Pleurotus geesteranus cultivation seeds are connected to the fungus bag in the inoculation room. After the inoculation is completed, the fungus bag is placed on the greenhouse grid for cultivation. The culture temperature is preferably 26°C, and it is cultured in the dark. The culture room is cultivated twice a day, each time for 30 minutes, and the relative humidity of the air is maintained at 70%. When the mycelium fills the fungus bag, it is the Pleurotus geesteranus fruiting fungus bag.

[0066] 3. Mushroom production management

[0067] For physiologically mature mushroom bags, the temperature of the mushroom house (shed) is reduced to 8℃ through mobile refrigeration equipment and maintained for 12 hours. The mushroom house (shed) is sealed for 3 days and the humidity is controlled at around 90% to promote primordium formation.

[0068] After the primordium is formed, give light with an intensity of 300lux-400lux, keep the temperature relatively stable, avoid large temperature fluctuations, and keep the humidity at 90%. When the stem of the small mushroom bud of Pleurotus geesteranus is 2cm, lift the film 40cm to ventilate; when the stem grows to 3cm and the cap diameter reaches about 2cm, lift the film to 1.5m, increase the ventilation, and keep the environmental humidity at 90%; and use a sprayer to spray water into the space, spray frequently and finely. At the same time, give continuous light to form commercial mushrooms.

[0069] Comparative Example 2:

[0070] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0071] Fruiting method 2: Factory-scale bottle cultivation, one-way fruiting.

[0072] 1. Preparation of liquid spawn of Pleurotus geesteranus

[0073] Preparation of liquid spawn of Pleurotus geesteranus:

[0074] Preparation of mother culture medium: Use PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose to the filtrate, make up to 1000ml, divide into packages, sterilize at 121℃ for 25min, and set aside after cooling.

[0075] Inoculation: Take 3 g of the vigorously growing Pleurotus geesteranus test tube culture medium, inoculate it into the above-mentioned mother culture medium, and culture it on a shaker (120r / min, 26°C). After 7 days, the Pleurotus geesteranus liquid spawn mother culture is ready.

[0076] 2. Production of liquid production of Pleurotus geesteranus

[0077] The formula of liquid production seed is: 20kg white cane sugar, 5kg glucose, 5kg soybean powder, 5kg corn powder, 5kg bran, 5kg yeast paste, 0.5kg potassium dihydrogen phosphate, 0.5kg magnesium sulfate, and 0.5kg defoaming agent. The production seed liquid culture medium is loaded into the fermentation tank in proportion. The fermentation tank with a volume of 1000L is loaded with 750L of liquid culture medium. The culture medium is sterilized at 121℃ for 35min. After the sterilization, wait for the culture medium temperature to drop to 20℃, and connect the liquid seed of Pleurotus geesteranus to the fermentation tank in the sterile room. Maintain the air intake pressure of the fermentation tank at 0.15MPa, and ensure that the air entering the tank is sterile, so that the liquid culture medium keeps rolling. The ambient temperature of the culture room is controlled at 20℃, and the air in the culture room is filtered through 100,000 grades and maintained at positive pressure. After 8 days of cultivation, the strain can be used for inoculation of bacteria bags.

[0078] 3. Production and sterilization of fungus bags

[0079] Formula for factory-based bottle cultivation of Pleurotus geesteranus: by mass ratio, 45% sawdust, 25% cottonseed hulls, 10% corn cobs, 18% bran, and 2% lime. Mix all kinds of raw materials thoroughly according to the formula ratio, and the water content reaches 63%. The bagging machine is used for bagging, and the total height of the bag is 20cm. The diameter of the reserved hole in the center of the bag is 2cm, and the hole depth is 18cm. After bagging, high-pressure sterilization (121℃, 2.5h) is carried out to avoid squeezing and deformation of the bag.

[0080] 4. Inoculation and cultivation

[0081] The liquid spawn of Pleurotus geesteranus is injected into the sterilized spawn bag from the reserved hole with an inoculation needle. After inoculation, the temperature of the culture room is set to 26°C for the first 5 days. After 5 days, the mycelium has been colonized and germinated. The temperature of the culture room is gradually lowered to 23°C. At the same time, the CO2 concentration in the culture room is controlled below 0.02%. During the spawning period, ventilation is continued to ensure that there is sufficient oxygen in the culture room.

[0082] 5. Mushroom production management

[0083] After the spawning bottles are put into frames (12 bottles per frame), they are moved into the factory-made mushroom warehouse. The temperature in the warehouse is lowered to 8℃ and maintained for 10 hours. The bottle caps are opened and the bottle mouths are scratched with a scratching machine. 20ml of water is added to the bottle mouths. The frames are then placed sideways so that the spawn bottles lie horizontally. The warehouse temperature is adjusted to 29℃, the CO2 concentration in the warehouse is increased to 0.2%, and ventilation is performed for 5 minutes per hour. The warehouse is kept dark, and primordia can be formed after 72 hours. After the primordia are formed, 300lux of light stimulation is applied to promote the differentiation of primordia. After the primordium stipes gradually elongate, the ventilation volume is increased, and the CO2 concentration is reduced to below 0.05%, so that high-quality fruiting bodies are formed.

[0084] Embodiment 1:

[0085] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0086] Mushroom production method 3: factory-style bag cultivation, two-way mushroom production.

[0087] 1. Preparation of liquid spawn of Pleurotus geesteranus

[0088] Preparation of liquid spawn of Pleurotus geesteranus:

[0089] Preparation of mother culture medium: Use PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose to the filtrate, make up to 1000ml, divide into packages, sterilize at 121℃ for 25min, and set aside after cooling.

[0090] Inoculation: Take 3 g of the vigorously growing Pleurotus geesteranus test tube culture medium, inoculate it into the above-mentioned mother culture medium, and culture it on a shaker (120r / min, 26°C). After 7 days, the Pleurotus geesteranus liquid spawn mother culture is ready.

[0091] 2. Production of liquid production of Pleurotus geesteranus

[0092] The formula of liquid production seed is: 22.5kg white cane sugar, 7.5kg glucose, 6kg soybean powder, 4.5kg corn flour, 5kg bran, 5kg yeast paste, 0.5kg potassium dihydrogen phosphate, 0.5kg magnesium sulfate, and 0.5kg defoamer. The production seed liquid culture medium is loaded into the fermentation tank in proportion. The fermentation tank with a volume of 1000L is loaded with 750L of liquid culture medium. The culture medium is sterilized at 121℃ for 35min. After the sterilization, wait for the culture medium temperature to drop to 20℃, and connect the liquid seed of Pleurotus geesteranus to the fermentation tank in the sterile room, maintain the air intake pressure of the fermentation tank at 0.15MPa, and ensure that the air entering the tank is sterile, so that the liquid culture medium keeps rolling. The ambient temperature of the culture room is controlled at 20℃, and the air in the culture room is filtered through 100,000 grades and maintained at positive pressure. After 8 days of cultivation, the strain can be used for inoculation of bacteria bags.

[0093] 3. Production and sterilization of fungus bags

[0094] Formula for factory-based bag cultivation of Pleurotus geesteranus: by mass ratio, 45% sawdust, 25% cottonseed hulls, 10% corn cobs, 18% bran, and 2% lime. Mix all kinds of raw materials thoroughly according to the formula ratio, and the water content reaches 63%. The bagging machine is used for bagging, and the total height of the bag is 20cm. The diameter of the reserved hole in the center of the bag is 2cm, and the hole depth is 18cm. After bagging, high-pressure sterilization (121℃, 2.5h) is carried out to avoid squeezing and deformation of the bag.

[0095] 4. Inoculation and cultivation

[0096] The liquid spawn of Pleurotus geesteranus was injected into the sterilized spawn bag from the reserved hole with an inoculation needle. After inoculation, the temperature of the culture room was set at 26°C for the first 5 days. After 5 days, the mycelium had been colonized and germinated. The temperature of the culture room was gradually lowered to 23°C. At the same time, the CO2 concentration in the culture room was controlled below 0.02%. During the spawning period, ventilation was continued to ensure that there was sufficient oxygen in the culture room.

[0097] 5. Mushroom production management

[0098] Move the mushroom bags that have been completed into the factory-made mushroom warehouse and place them on the grid rack on their sides. Lower the temperature in the warehouse to 6°C and keep it for 10 hours. Since the mycelium age at both ends of the mushroom bag is the same, cut both ends of the mushroom bag at the same time and spray water on both ends of the mushroom bag once to ensure that the surface of the mushroom bag is moist after the cut, increase the humidity in the warehouse and maintain it above 95%; raise the temperature in the warehouse to 28°C; increase the CO2 concentration in the warehouse to 0.2%, and ventilate for 10 minutes per hour; keep the warehouse dark, and primordium will form after 72 hours. After the primordium is formed, give 350lux light stimulation to promote primordium differentiation; after the primordium stipe gradually elongates, increase the ventilation volume, and reduce the CO2 concentration to 0.03% to form high-quality fruiting bodies. The yield of one wave of mushrooms can reach more than twice that of factory-made bottle cultivation, achieving high efficiency and high yield.

[0099] Comparison of different production processes and mushroom production methods

[0100]

[0101]

[0102] Note: The inoculation described in the table refers to the use of production species to inoculate the fruiting bags. Pleurotus geesteranus strains are divided into three levels: mother strain (first-level strain), original strain (second-level strain), and production strain, also called cultivated strain (third-level strain). When making fruiting bags, the production strain (third-level strain) is used for inoculation.

[0103] Comparative Example 3

[0104] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0105] Mushroom production method 1: Steel frame greenhouse, low temperature in summer stimulates mushroom production.

[0106] 1. Preparation of solid spawn of Pleurotus geesteranus

[0107] Preparation of mother culture: PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose and 13g of agar powder to the filtrate, make up to 1000ml, divide into test tubes, sterilize at 121℃ for 25min, then arrange the test tubes into slant culture medium and cool for use.

[0108] Inoculation: Take a 0.5cm×0.5cm block of Pleurotus geesteranus culture and inoculate it into the above slant culture medium. Culture it at 26℃ for 10 days. When the mycelium covers the entire slant, it is the mother culture of Pleurotus geesteranus.

[0109] Original seed production:

[0110] The original seed culture matrix is ​​configured as follows: 40% cottonseed hull, 40% sawdust (not included in Example 1), 18% bran, 1% sugar, and 1% lime. First, weigh the raw and auxiliary materials such as cottonseed hull, sawdust, bran, and lime according to the mass ratio, mix and add water while stirring, and ensure that the stirring is sufficient so that the water content of the culture material reaches 63%. After the preparation is completed, the mixture is immediately bagged (bottle) and sterilized, and the mixture is sterilized by high pressure for 2 hours. When the mixture is cooled to 26°C, the mother species of Pleurotus geesteranus is connected to the bacteria bag or bacteria bottle in the clean bench, inoculation box or inoculation room. After the inoculation is completed, the mixture is moved into the culture room for cultivation. The culture temperature is preferably 26°C. The culture room is ventilated twice a day for 30 minutes each time, and the relative humidity of the air is maintained at 70%. When the mycelium fills the bacteria bottle or the bacteria bag, the original species of Pleurotus geesteranus is obtained.

[0111] Cultivated species production:

[0112] Cultivation culture medium configuration: 40% cottonseed hulls, 40% sawdust, 18% bran, 2% lime. First weigh the raw and auxiliary materials such as cotton hulls, sawdust, bran, lime, etc. according to the mass ratio, mix them and add water while stirring, ensure sufficient stirring, and make the water content of the culture material reach 63%. After the preparation is completed, immediately bag and sterilize, autoclave for 2 hours, and cool to 26℃, and then connect the original species of Pleurotus geesteranus to the fungus bag in the clean bench, inoculation box or inoculation room. After inoculation, move it to the culture room for cultivation. The culture temperature is preferably 26℃. The culture room is twice a day, 30 minutes each time, and the relative humidity of the air is maintained at 70%. When the mycelium fills the fungus bag, it is the cultivation species of Pleurotus geesteranus.

[0113] 2. Production of Pleurotus geesteranus mushroom bags

[0114] Formula for the cultivation of Pleurotus geesteranus in steel frame greenhouse: by mass ratio, 35% sawdust, 15% mulberry branch, 20% cottonseed hull, 12% corn cob, 16% bran, 2% lime. First weigh the raw and auxiliary materials such as cotton hull, sawdust, bran, corn flour, gypsum, lime, etc. by mass ratio, mix them and add water while stirring, ensure sufficient stirring, so that the water content of the culture material reaches 65%. After the preparation is completed, immediately bag and sterilize, autoclave for 2 hours, cool to 26℃, and connect the Pleurotus geesteranus cultivation seeds to the fungus bag in the inoculation room. After the inoculation is completed, place the fungus bag on the greenhouse grid for cultivation. The cultivation temperature is preferably 26℃, and it is cultivated in the dark. The culture room is cultivated twice a day, 30 minutes each time, and the relative humidity of the air is maintained at 70%. When the mycelium fills the fungus bag, it is the fruiting fungus bag of Pleurotus geesteranus.

[0115] 3. Mushroom production management

[0116] For physiologically mature mushroom bags, the temperature of the mushroom house (shed) is reduced to 6°C through mobile refrigeration equipment and maintained for 12 hours. The mushroom house (shed) is sealed for 3 days and the humidity is controlled at around 90% to promote primordium formation.

[0117] After the primordium is formed, light is applied at an intensity of 450 lux, the temperature is kept relatively stable, and large temperature fluctuations are avoided. The humidity is kept at 90%. When the stem of the small mushroom bud of Pleurotus geesteranus is 2 cm, the film hanging on the ground is lifted up by 0.5 m for ventilation; when the stem grows to 3 cm and the diameter of the cap is about 2 cm, the film is lifted up to 1.8 m, the ventilation is increased, and the environmental humidity is kept at 90%. Water is sprayed into the space with a sprayer, and it is sprayed frequently and finely. At the same time, continuous light is applied to form commercial mushrooms.

[0118] Comparative Example 4

[0119] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0120] Fruiting method 2: Factory-scale bottle cultivation, one-way fruiting.

[0121] 1. Preparation of liquid spawn of Pleurotus geesteranus

[0122] Preparation of liquid spawn of Pleurotus geesteranus:

[0123] Preparation of mother culture medium: Use PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, boil, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose to the filtrate, make up to 1000ml, divide into packages, sterilize at 121℃ for 25min, and set aside after cooling.

[0124] Inoculation: Take 3 g of the vigorously growing Pleurotus geesteranus test tube culture medium, inoculate it into the above-mentioned mother culture medium, and culture it on a shaker (120r / min, 26°C). After 7 days, the Pleurotus geesteranus liquid spawn mother culture is ready.

[0125] 2. Production of liquid production of Pleurotus geesteranus

[0126] The formula of liquid production seed is: 22.5kg white cane sugar, 7.5kg glucose, 7.5kg soybean powder, 5kg corn flour, 5kg bran, 5kg yeast paste, 0.75kg potassium dihydrogen phosphate, 0.75kg magnesium sulfate, and 0.5kg defoamer. The production seed liquid culture medium is loaded into the fermentation tank in proportion. The fermentation tank with a volume of 1000L is loaded with 800L of liquid culture medium. The culture medium is sterilized at 121℃ for 35min. After the sterilization, wait for the culture medium temperature to drop to 20℃, and connect the liquid seed of Pleurotus geesteranus to the fermentation tank in the sterile room, maintain the air intake pressure of the fermentation tank at 0.15MPa, and ensure that the air entering the tank is sterile, so that the liquid culture medium keeps rolling. The ambient temperature of the culture room is controlled at 20℃, and the air in the culture room is filtered through 100,000 grades and maintained at positive pressure. After 8 days of cultivation, the strain can be used for inoculation of bacteria bags.

[0127] 3. Production and sterilization of fungus bags

[0128] Formula for the factory-based cultivation of Pleurotus geesteranus: by mass ratio, 35% sawdust, 15% mulberry branch chips, 20% cottonseed hulls, 12% corn cobs, 16% bran, and 2% lime. Mix all the raw materials thoroughly according to the formula ratio, and the water content reaches 63%. The bags are packed by a bagging machine, with a total height of 20cm, a reserved hole in the center of the bag with a diameter of 2cm, and a hole depth of 18cm. After bagging, high-pressure sterilization (121℃, 2.5h) is carried out to prevent the bags from being squeezed and deformed.

[0129] 4. Inoculation and cultivation

[0130] The liquid spawn of Pleurotus geesteranus was injected into the sterilized spawn bag from the reserved hole with an inoculation needle. After inoculation, the temperature of the culture room was set at 26°C for the first 5 days. After 5 days, the mycelium had been colonized and germinated. The temperature of the culture room was gradually lowered to 23°C. At the same time, the CO2 concentration in the culture room was controlled below 0.02%. During the spawning period, ventilation was continued to ensure that there was sufficient oxygen in the culture room.

[0131] 5. Mushroom production management

[0132] After the spawning bottles are put into frames (12 bottles per frame), they are moved into the factory-made mushroom warehouse. The temperature in the warehouse is lowered to 6℃ and maintained for 10 hours. The bottle caps are opened and the bottle mouths are scratched with a scratching machine. 20ml of water is added to the bottle mouths. The frames are then placed sideways so that the spawn bottles lie horizontally. The warehouse temperature is adjusted to 28℃, the CO2 concentration in the warehouse is increased to 0.2%, and ventilation is performed for 8 minutes per hour. The warehouse is kept dark, and primordia can be formed after 72 hours. After the primordia are formed, 350lux light stimulation is applied to promote the differentiation of primordia. After the primordium stipes gradually elongate, the ventilation volume is increased, and the CO2 concentration is reduced to below 0.05%, so that high-quality fruiting bodies are formed.

[0133] Example 2

[0134] Pleurotus geesteranus variety: 'Hangxiu No. 2'.

[0135] Mushroom production method 3: factory-style bag cultivation, two-way mushroom production.

[0136] 1. Preparation of liquid spawn of Pleurotus geesteranus

[0137] Preparation of liquid spawn of Pleurotus geesteranus:

[0138] Preparation of mother culture medium: Use PDA liquid culture medium, take 200g of fresh potatoes, peel and cut into strips (slices), add 1000ml of water, filter with gauze when the potatoes are cooked but not mushy, add 20g of glucose to the filtrate, make up to 1000ml, divide into portions, sterilize at 121℃ for 25min, and set aside after cooling.

[0139] Inoculation: Take 2 g of the vigorously growing Pleurotus geesteranus test tube culture medium, inoculate it into the above-mentioned mother culture medium, and culture it on a shaker (120r / min, 26°C). After 7 days, the Pleurotus geesteranus liquid spawn mother culture is ready.

[0140] 2. Production of liquid production of Pleurotus geesteranus

[0141] The formula of liquid production seed is: 25kg white cane sugar, 10kg glucose, 7.5kg bean powder, 5kg corn powder, 5kg bran, 5kg yeast paste, 0.75kg potassium dihydrogen phosphate, 0.75kg magnesium sulfate, 0.5kg defoamer. The production seed liquid culture medium is loaded into the fermentation tank in proportion. The fermentation tank with a volume of 1000L is loaded with 800L of liquid culture medium. The culture medium is sterilized at 121℃ for 35min. After the sterilization, wait for the culture medium temperature to drop to 20℃, and connect the liquid seed of Pleurotus geesteranus to the fermentation tank in the sterile room. Maintain the inlet pressure of the fermentation tank at 0.15MPa, and ensure that the air entering the tank is sterile, so that the liquid culture medium keeps rolling. The ambient temperature of the culture room is controlled at 18℃, and the air in the culture room is filtered through 100,000 grades and maintained at positive pressure. After 10 days of cultivation, the strain can be used for inoculation of bacteria bags.

[0142] 3. Production and sterilization of fungus bags

[0143] Formula for factory-based bag cultivation of Pleurotus geesteranus: by mass ratio, 35% sawdust, 15% mulberry branch chips, 20% cottonseed hulls, 12% corn cobs, 16% bran, and 2% lime. Mix all kinds of raw materials thoroughly according to the formula ratio, and the water content reaches 64%. The bagging machine is used for bagging, the total height of the bag is 20cm, the diameter of the reserved hole in the center of the bag is 2cm, and the hole depth is 18cm. After bagging, high-pressure sterilization (121℃, 2.5h) is carried out to avoid squeezing and deformation of the bag.

[0144] 4. Inoculation and cultivation

[0145] The liquid spawn of Pleurotus geesteranus was injected into the sterilized spawn bag from the reserved hole with an inoculation needle. After inoculation, the temperature of the culture room was set at 26°C for the first 5 days. After 5 days, the mycelium had been colonized and germinated. The temperature of the culture room was gradually lowered to 23°C. At the same time, the CO2 concentration in the culture room was controlled below 0.02%. During the spawning period, ventilation was continued to ensure that there was sufficient oxygen in the culture room.

[0146] 5. Mushroom production management

[0147] Move the mushroom bags that have been fermented into the factory-made mushroom warehouse, lower the temperature in the warehouse to 8℃ and keep it for 10 hours. At the same time, cut the two ends of the mushroom bag, spray water on both ends of the mushroom bag once to ensure that the surface of the mushroom bag is moist after the cut, increase the humidity in the warehouse, and maintain it above 95%; raise the temperature in the warehouse to 28℃; increase the CO2 concentration in the warehouse to 0.2%, and ventilate for 8 minutes per hour; keep the warehouse dark, and primordium will be formed after 70 hours. Give 400lux light stimulation to promote primordium differentiation; after the primordium stipe gradually elongates, increase the ventilation volume, and reduce the CO2 concentration to 0.03% to form high-quality fruiting bodies. The yield of one wave of mushrooms can reach more than twice that of factory-made bottle cultivation, achieving high efficiency and high yield.

[0148] Comparison of different production processes and mushroom production methods in Example 2 and Comparative Examples 3 and 4

[0149]

[0150] Note: The inoculation described in the table refers to the use of production species to inoculate the fruiting bags. Pleurotus geesteranus strains are divided into three levels: mother strain (first-level strain), original strain (second-level strain), and production strain, also called cultivated strain (third-level strain). When making fruiting bags, the production strain (third-level strain) is used for inoculation.

[0151] The factory-based bag cultivation and two-way mushroom production method not only has a fast mushroom bag production speed and a high yield rate, but also the output of first-time mushrooms is significantly higher than that of Method 1 and Method 2. At the same time, the production cycle is short, and multiple productions can be achieved throughout the year. The production efficiency is significantly higher than that of Method 1, which can only produce one season throughout the year; compared with production method 2, the output and efficiency have also increased by more than 2 times.

[0152] Therefore, through this method, the contradiction between the output and efficiency of Pleurotus geesteranus factory production can be solved, thereby achieving high-yield and high-efficiency Pleurotus geesteranus cultivation.

[0153] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for cultivating Pleurotus geesteranus in an industrial scale, characterized in that: The method comprises the following steps: S1: Preparation of liquid spawn of Pleurotus geesteranus; S101: preparing mother culture medium; S102: inoculating the mother culture medium with Pleurotus geesteranus test tube cultures; S2: Production of liquid production of Pleurotus geesteranus; S3: Preparation and sterilization of fungus bags; S4: inoculation and cultivation; S5: mushroom production management; Step S101 of preparing the mother culture medium specifically includes: using PDA liquid culture medium, taking fresh potatoes, peeling them, cutting them into strips or slices, adding water and boiling them until the potatoes are cooked but not mushy, filtering them with gauze, adding glucose to the filtrate, fixing the volume, packaging and sterilizing them, cooling them and setting them aside; Step S102 of inoculating the Pleurotus geesteranus test tube seed in the mother seed culture medium specifically comprises: taking the Pleurotus geesteranus test tube seed with vigorous growth, inoculating it into the above-mentioned mother seed culture medium, and culturing it on a shaking table, so that the Pleurotus geesteranus liquid spawn mother seed is prepared, and the conditions for the shaking culture on the shaking table are 120r / min and 26°C; Step S3 of making and sterilizing the fungus bag specifically includes: S301: equipped with bag cultivation formula, the bag cultivation formula includes: 45% sawdust, 25% cottonseed hull, 10% corn cob, 18% bran, 2% lime; or, 35% sawdust, 15% mulberry branch, 20% cottonseed hull, 12% corn cob, 16% bran, 2% lime; S302: preparing mushroom bags: stirring the above formula to make its water content reach 63-65%, and leaving a hole in the center of the mushroom bag after bagging, with a hole diameter of 2 cm and a hole depth of 17-18 cm. After bagging, high pressure sterilization is performed; Step S4 of inoculation and culturing specifically includes: injecting the liquid spawn of Pleurotus geesteranus into the sterilized spawn bag from the reserved hole with an inoculation needle, setting the temperature of the culturing room to 26° C. for the first 3 to 5 days after inoculation, gradually lowering the temperature of the culturing room to 23° C. after 5 days when the mycelium has been planted and germinated, and controlling the CO2 concentration in the culturing room to below 0.02%, and continuously ventilating during the spawning period to ensure that the culturing room has sufficient oxygen; Step S5 mushroom production management specifically includes: S501: Move the mushroom bags that have been fermented into the factory-made mushroom warehouse and place them on their sides on the grid racks. The temperature in the warehouse is lowered to 5-8°C. At the same time, cut the ends of the side-lying mushroom bags to expose the material surface. S502: The temperature of the warehouse is raised to 28±2℃, the CO2 concentration in the warehouse is increased to 0.2%, ventilation is carried out for 5 to 10 minutes per hour, and after 70 to 72 hours, the primordium is formed; S503: After the primordium is formed, 300 lux to 400 lux light stimulation is given to promote primordium differentiation; S504: When the stipes of the small mushroom buds of Pleurotus geesteranus are 1 to 2 cm long, the CO2 concentration in the warehouse is reduced to 0.05% to 0.1%; when the stipes grow to 3 to 4 cm and the cap diameter reaches 2 cm, increase the ventilation volume to reduce the CO2 concentration in the warehouse to below 0.05%, maintain the light intensity at 450lux to 500lux, and control the warehouse temperature at 24±1℃ to promote the formation of high-quality fruiting bodies.

2. The method for cultivating Pleurotus geesteranus in an industrial scale according to claim 1, characterized in that: Step S2 of preparing the liquid production seed of Pleurotus geesteranus specifically includes: S201: Prepare liquid culture medium: select a liquid production formula and add water to 750L to 800L; S202: The above formula is loaded into a fermentation tank for fermentation.

3. The method for cultivating Pleurotus geesteranus in an industrial scale according to claim 2, characterized in that: The liquid production formula in step S201 includes, by weight, 20kg to 25kg of white sucrose, 5kg to 10kg of glucose, 5kg to 7.5kg of soybean powder, 4kg to 5kg of corn powder, 4kg to 5kg of bran, 5kg of yeast extract, 0.5kg to 1kg of potassium dihydrogen phosphate, 0.5kg to 1kg of magnesium sulfate, and 0.5kg of defoaming agent.

4. The method for cultivating Pleurotus geesteranus in an industrial scale according to claim 3, characterized in that: The fermentation in the fermentation tank in step S202 specifically includes: loading the liquid culture medium into the fermentation tank according to proportion, culturing and sterilizing, and after the sterilization is completed, waiting for the temperature to drop to 20° C., connecting the liquid spawn of Pleurotus geesteranus to the fermentation tank in a sterile room, maintaining the air inlet pressure of the fermentation tank at 0.15 MPa, and ensuring that the air in the fermentation tank is sterile, ensuring that there is sufficient oxygen during the mycelium fermentation process, and causing the liquid culture medium to roll.

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