Plant secretion collection device

Abstract

The present invention provides a plant secretion collection device, comprising an incubator, wherein the incubator is provided with multiple soil culture tanks arranged in parallel, a centrifugal tank disposed below the soil culture tanks, a first solenoid valve disposed at the lower end of the soil culture tanks and connected to the centrifugal tanks, and a cover plate disposed at the upper end of the soil culture tanks, a push rod fixedly connected to the cover plate, the end of the push rod remote from the cover plate being fixed to the incubator, and a vibrator and a deionized water pipe fixedly mounted on the cover plate. The purpose of the device is to automatically extract plant secretions while avoiding root damage during the extraction process.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cultivation experimental equipment, in particular to a plant secretion collecting device. Background Art

[0002] Roots are crucial organs for plants to exchange substances and energy with the external environment. Root exudates are a mixture of diverse and varying amounts of primary metabolites such as sugars, amino acids, and organic acids, secondary metabolites such as phenols, and some unidentified metabolites released by plant roots into the surrounding environment through metabolic and non-metabolic pathways. Root exudates are a normal physiological phenomenon, primarily providing energy and information for plant growth, development, and reproduction, as well as for adaptation to adverse environments. Root exudates are one of the primary ways plants influence the soil environment. Their synthesis, release, dynamics of growth and decline in the rhizosphere, and their impact on soil properties, microbial activity, and plant growth and development all hold important theoretical and practical significance.

[0003] At present, the general method for collecting plant roots is solution culture collection, soil culture collection and matrix culture collection. In the solution culture method, the root system grows under waterlogging, and the root system growth resistance is small, which is quite different from the actual situation of root secretions under soil cultivation conditions. The soil culture collection method is more troublesome than solution culture collection, but it can better reflect the actual situation of plants in the soil. However, the soil culture collection process is cumbersome and requires extraction of rhizosphere soil. After the rhizosphere soil is mixed with deionized water, the supernatant is centrifuged and tested after treatment. However, current soil culture collection is mostly manually carried out, and the process is troublesome. Because the extraction of rhizosphere soil requires washing the roots with water, experienced staff need to manually operate. However, even if manually operated, the roots may be damaged during the leaching process, resulting in the secretion of wound fluid containing the roots. Summary of the Invention

[0004] The present invention provides a plant secretion collection device, the purpose of which is to automatically extract plant secretions and avoid damaging the root system during the extraction.

[0005] The embodiments of the present invention are achieved through the following technical solutions:

[0006] A plant secretion collection device includes an incubator, wherein a plurality of soil culture tanks are arranged in parallel, and a centrifugal tank is arranged below the soil culture tanks.

[0007] A first solenoid valve is provided at the lower end of the soil culture tank and is connected to the centrifugal tank. A cover plate is provided at the upper end of the soil culture tank 0, and an electric push rod is fixedly connected to the cover plate. The end of the electric push rod away from the cover plate is fixed on the incubator, and an exciter and a deionized water pipe are fixedly installed on the cover plate.

[0008] Furthermore, the centrifugal tank is conical, a second solenoid valve is installed at the bottom of the centrifugal tank, a supernatant suction pipe is connected to the centrifugal tank, and a slag discharge pipe is connected to the bottom of the incubator.

[0009] Furthermore, a plurality of solution culture tanks are provided in the incubator, the solution culture tanks are arranged in parallel with the soil culture tanks 5, and a third electromagnetic valve is installed at the bottom of the solution culture tanks.

[0010] The upper end of the solution culture tank is connected with a liquid infusion tube.

[0011] Furthermore, a sterile filter is installed at the outlet end of the infusion tube.

[0012] Furthermore, a glass bead layer, a glass sand layer and a glass wool layer are sequentially arranged at the bottom of the solution culture tank.

[0013] 0 Further, the incubator is equipped with a culture lamp and an oxygen supply

[0014] Box and exhaust gas collection box.

[0015] The technical solutions of the embodiments of the present invention have at least the following advantages and beneficial effects:

[0016] Fix the plant with a cover plate. After most of the soil near the roots of the plant is washed with clean water, use an electric push rod and a vibrator to complete vibration screening of the plant, leaving the rhizosphere soil that is more tightly bonded to the roots, and discharge the excess soil. The rhizosphere soil is soaked in deionized water, and the vibrator is used to vibrate the rhizosphere soil again in the water to shake it off into the deionized water for standby use. The rhizosphere soil is obtained by vibration, which is not easy to cause root damage and cause the secretions to contain root bleeding fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 It is a structural schematic diagram of the present invention;

[0019] Icons: 1-incubator, 2-soil culture tank, 3-solution culture tank, 4-culture lamp, 5-oxygen supply box, 6-cover, 7-electric push rod, 8-deionized water pipe, 9-vibrator, 10-flushing pipe, 11-first solenoid valve, 12-centrifugal tank, 13-drive motor, 14-second solenoid valve, 15-supernatant suction pipe, 16-slag discharge pipe, 17-culture solution extraction pipe, 18-third battery valve, 19-waste gas collection box. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0021] Example 1

[0022] A plant secretion collection device includes an incubator 1, which is provided with two soil culture tanks 2 and two solution culture tanks 3. A first solenoid valve 11 is installed at the bottom of each soil culture tank 2, and a common cover plate 6 is installed on the top of the two soil culture tanks 1. An electric push rod 7 and an exciter 9 are installed on the cover plate 6. The output end of the electric push rod 7 is fixed to the cover plate 6, and the driving end of the electric push rod 7 is fixed to the top wall of the incubator 1. A deionized water pipe 8 is fixed to the cover plate 6, and the outlet end of the deionized water pipe 8 is directed toward the root position of the plant in the soil culture tank 1. A flushing pipe 10 is connected to the interior of the soil culture tank 2, and the outlet end of the flushing pipe 10 is directed toward the side wall of the soil culture tank 2. A centrifuge tank 12 is installed below the soil culture tank 2 and the solution culture tank 3 in the incubator 1. The centrifuge tank 12 is driven by a drive motor 13. A second battery valve is installed at the bottom of the centrifuge tank 12. A supernatant suction pipe 15 is connected to the centrifuge tank 12, and a slag discharge pipe 16 and a culture solution collection pipe are connected to the bottom of the incubator 1.

[0023] Holes are provided on the cover plate 6 and the upper end of the solution culture tank 3. Suitable plants are passed through the holes and the trunk of the plants are fixed in the holes. Nutrient solution is added to the plants in the solution culture tank 3 for cultivation, and soil is filled in the soil culture tank 2 for cultivation. After a period of cultivation, the first solenoid valve 11 is opened, and the soil culture tank 2 is flushed with water using the flushing pipe 10. The flushing pipe 10 is aligned with both sides of the soil culture tank 2 to flush the soil away from the roots to the bottom of the culture box 1 and discharged through the slag discharge pipe 16. After flushing for a period of time, the soil adhering to the roots of the plants is reduced, and the electric push rod 7 is used to lift the cover plate 6 and the plants. The vibrator 9 is used to vibrate the roots of the plants. Since the soil is closer to the roots, the tighter it is, the excess soil is removed by vibration to retain the rhizosphere soil. After the excess soil is flushed away by the flushing pipe 10, only part of the rhizosphere soil remains at the roots of the plants. Use the electric push rod 7 to push back the cover 6 and the plant, close the first solenoid valve 11, and use the deionized water pipe 8 to input deionized water into the soil culture tank 2. The deionized water soaks the roots of the plant. Then turn on the vibrator 9 and use vibration to separate the rhizosphere soil. The rhizosphere soil is mixed with the deionized water, and then the deionized water and rhizosphere soil are input into the centrifugal tank 12. After centrifugation, the supernatant is taken out with the supernatant suction tube 15 for testing.

[0024] A culture lamp 4 is installed in the incubator 1, and an oxygen supply box 5 and an exhaust gas collection box 19 are installed on the incubator 1, so that the soil culture tank 2 and the solution culture tank 3 are in the same growth environment to ensure control conditions.

[0025] The bottom of the solution culture tank is sequentially provided with a glass bead layer, a glass sand layer and a glass wool layer, which is convenient for the initial filtration of the nutrient solution.

[0026] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A plant secretion collection device, characterized in that: The invention comprises an incubator (1), wherein a plurality of soil culture tanks (2) are arranged in parallel in the incubator (1), a centrifugal tank (12) is arranged below the soil culture tank (2), a first electromagnetic valve (11) is arranged at the lower end of the soil culture tank (2) and is connected to the centrifugal tank (12), a cover plate (6) is arranged at the upper end of the soil culture tank (2), an electric push rod (7) is fixedly connected to the cover plate (6), an end of the electric push rod (7) away from the cover plate (6) is fixed to the incubator (1), and an exciter (9) and a deionized water pipe (8) are fixedly installed on the cover plate (6); The driving end of the electric push rod (7) is fixed to the top wall of the incubator (1), a deionized water pipe (8) is fixed to the cover plate (6), the outlet end of the deionized water pipe (8) is directed toward the root position of the plant in the soil incubator (1), and a flushing pipe (10) is connected to the interior of the soil incubator (2), the outlet end of the flushing pipe (10) is directed toward the side wall of the soil incubator (2); The centrifugal tank (12) is conical, a second solenoid valve (14) is installed at the bottom of the centrifugal tank (12), a supernatant suction pipe (15) is connected to the inside of the centrifugal tank (12), and a slag discharge pipe (16) is connected to the bottom of the incubator (1).

2. A plant secretion collection device according to claim 1, characterized in that: A plurality of solution culture tanks (3) are provided in the culture box (1), the solution culture tanks (3) and the soil culture tanks (2) are arranged in parallel, a third solenoid valve is installed at the bottom of the solution culture tanks (3), and a liquid infusion tube is connected to the upper end of the solution culture tanks (3).

3. A plant secretion collection device according to claim 2, characterized in that: A sterile filter is installed at the outlet end of the infusion tube.

4. The plant secretion collection device according to claim 2, characterized in that: The bottom of the solution culture tank (3) is provided with a glass bead layer, a glass sand layer and a glass wool layer in sequence.

5. The plant secretion collection device according to claim 1, characterized in that: A culture lamp (4) is installed in the incubator (1), and an oxygen supply box (5) and a waste gas collection box (19) are installed on the incubator (1).

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