An automatic staining module and staining method for formed components of gynecological secretions

Abstract

The present invention relates to the technical field of automatic cell staining for medical devices, and particularly relates to an automatic staining module and staining method for formed components of gynecological secretions, including a conveyor belt, a steel needle mounting seat, reagent dropping steel needles, set screws, a reagent dropping cover mounting plate, a reagent dropping cover, a waste liquid sampling needle, a waste liquid sampling needle baffle, a spring, a glass slide, and a glass slide lifting box. The conveyor belt is arranged directly below the reagent dropping cover, and the glass slide lifting box is installed directly below the reagent dropping cover. There are three reagent dropping steel needles, and the three reagent dropping steel needles are installed on the steel needle mounting seat through set screws. The steel needle mounting seat and the reagent dropping cover are installed on the reagent dropping cover mounting plate, and the three reagent dropping steel needles are inserted into the interior of the reagent dropping cover. The waste liquid sampling needle is movably inserted into the steel needle mounting seat and the reagent dropping cover. The protrusion on the waste liquid sampling needle cooperates with the waste liquid sampling needle baffle installed, and the spring is sleeved on the waste liquid sampling needle, making the staining uniform, the process environmentally friendly, and the speed faster.

Description

Technical Field

[0001] The present invention relates to the technical field of automatic cell staining of medical devices, and particularly to an automatic staining module and staining method for formed elements in gynecological secretions. Background Art

[0002] Ordinary manual Giemsa staining method: 1. Drop about 0.5 ml - 0.8 ml of staining solution A on the glass slide with the solidified sample and stain for 1 minute. 2. Drop staining solution B on top of staining solution A (the dropping amount is 2 - 3 times that of staining solution A), and use an ear bulb to blow a gentle breeze to make ripples on the liquid surface to fully mix the two liquids, and stain for 4 - 10 minutes. 3. Wash with water.

[0003] In the existing automatic staining of formed elements in gynecological secretions, one is an automatic staining similar to manual Swiss Giemsa staining. Since the water absorption of each area of the sample after drying is different, when the machine stains, it cannot autonomously judge the uniformity of the stain coverage like a human, and automatically adjust the position of dropping the stain. Due to the different water absorption of the sample, when dropping the sample obliquely (if completely following the manual staining method, the liquid is likely to flow to the back of the glass slide, which is not conducive to realizing automated operation), the stain directly flows away, resulting in uneven staining (some positions are stained with A, some positions are stained with B, some positions are deeply stained, and some positions are not stained); at the same time, this method is based on the Swiss Giemsa staining process, using two staining solutions A and B. After dropping A, wait for the stain to act on the cells, rinse, then drop B, wait for the stain to act on the cells, and then rinse again. The staining time is relatively long, which is not conducive to speeding up the entire instrument from sampling to outputting a report.

[0004] Another one is a process similar to Swiss Giemsa staining, but uses a disposable container to completely immerse the glass slide carrying the dried cell sample for staining. However, in order to prevent cross - contamination problems, the used container is disposable. Although the staining effect is good, it causes great material waste, and in order to be able to completely immerse the glass slide, the use of this chemical drug (the base liquid is methanol) for the stain is increased, not only increasing the cost, but also not meeting the national requirements for energy conservation, emission reduction and environmental protection. Summary of the Invention

[0005] The purpose of the present invention is to provide an automatic staining module and staining method for formed elements in gynecological secretions, with uniform staining, an environmentally friendly process, and faster speed.

[0006] To achieve the above object, the present invention provides an automatic staining module for formed components of gynecological secretions, including a conveyor belt, a steel needle mounting seat, reagent dropping steel needles, set screws, a reagent dropping cover mounting plate, a reagent dropping cover, a waste liquid sampling needle, a waste liquid sampling needle baffle, a spring, a glass slide, and a glass slide lifting box. The conveyor belt is disposed directly below the reagent dropping cover. The glass slide lifting box is mounted directly below the reagent dropping cover. The number of the reagent dropping steel needles is three. The three reagent dropping steel needles are mounted on the steel needle mounting seat through the set screws. The steel needle mounting seat and the reagent dropping cover are mounted on the reagent dropping cover mounting plate, and the three reagent dropping steel needles are inserted into the interior of the reagent dropping cover. The waste liquid sampling needle is movably inserted into the steel needle mounting seat and the reagent dropping cover. A protrusion on the waste liquid sampling needle cooperates with the waste liquid sampling needle baffle mounted thereon. The spring is sleeved on the waste liquid sampling needle.

[0007] Wherein, the reagent dropping cover is provided with an opening inclined surface and a guiding groove.

[0008] Wherein, a bottom skirt is provided at the bottom of the reagent dropping cover.

[0009] Wherein, side baffles are provided on both sides of the reagent dropping cover.

[0010] Wherein, the bottom of the reagent dropping cover is a first inclined surface, and the angle of the first inclined surface is 9°.

[0011] Wherein, the glass slide lifting box is provided with a second inclined surface having the same inclination angle as that of the first inclined surface.

[0012] The present invention also provides a staining method for the automatic staining module for formed components of gynecological secretions as described above, specifically including the following steps:

[0013] The glass slide with the sample dried and cured at high temperature is transported by the synchronous belt to directly below the reagent dropping cover;

[0014] The glass slide lifting box lifts the glass slide upward. Since the glass slide lifting box has a second inclined surface, the glass slide can simultaneously obtain an angle parallel to the first inclined surface of the reagent dropping cover. The glass slide lifting box continues to lift, causing the glass slide to contact the reagent dropping cover and deforming the bottom skirt of the reagent dropping cover, resulting in a closed cavity at the bottom formed by the reagent dropping cover and the glass slide that contains the sample. At this time, the waste liquid sampling needle is pushed upward by the glass slide. Due to the action of the spring, the bottom of the waste liquid sampling needle closely adheres to the glass slide;

[0015] Start dropping the staining agent. Since a closed cavity is formed at the lower side by the glass slide and the reagent dropping cover at this time, drop the staining agent C and it accumulates in this cavity and finally completely submerges the sample on the glass slide.

[0016] Wait for the dye C to react with the sample; start dropping and rinsing with purified water. After the purified water starts to rinse, the pump connected to the waste liquid sampling needle starts to work, sucking away the dye, purified water, and a small part of the sample detached from the glass slide in the cavity.

[0017] Subsequently, the glass slide is taken off as the glass slide lifting box descends, disengaging from the bottom skirt of the reagent dropping cover by about 2 mm, allowing some purified water to overflow from this gap.

[0018] The liquid is drained through the side baffle and flows into the waste liquid storage chamber of the glass slide lifting box. Finally, the bottom of the glass slide lifting box sucks away these waste liquids to complete the staining of one sample.

[0019] An automatic staining module and staining method for formed components of gynecological secretions according to the present invention use the glass slide itself and the reagent dropping cover to jointly form a cavity sealed at the lower end, enabling the sample to be immersed in the dye inside and facilitating the cleaning of the reagent dropping cover after detachment. During operation, the actions are simple and the time used is short, which is conducive to the realization of rapid automation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art.

[0021] Figure 1 is an axonometric view of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0022] Figure 2 is an internal view of the steel needle inserted into the reagent dropping cover of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0023] Figure 3 is a front view of the reagent dropping cover of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0024] Figure 4 is a left view of the reagent dropping cover of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0025] Figure 5 is a schematic diagram of the glass slide lifting box of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0026] Figure 6 is a flowchart of an automatic staining module and staining method for formed components of gynecological secretions according to the present invention.

[0027] 1 - conveyor belt, 2 - steel needle mounting base, 3 - reagent dropping steel needle, 4 - set screw, 5 - reagent dropping cover mounting plate, 6 - reagent dropping cover, 7 - waste liquid sampling needle, 8 - waste liquid sampling needle baffle, 9 - spring, 10 - opening inclined plane, 11 - guiding groove, 12 - glass slide, 13 - glass slide lifting box, 14 - bottom skirt, 15 - side baffle, 16 - first inclined plane, 17 - second inclined plane. Detailed implementation manners

[0028] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present invention, and should not be construed as a limitation to the present invention.

[0029] Please refer to Figures 1 to 5 , Figure 1 is an axonometric view of an automatic staining module for formed components of gynecological secretions according to the present invention, Figure 2 is an internal view of the steel needle inserted into the reagent dropping cover 6 of an automatic staining module for formed components of gynecological secretions according to the present invention, Figure 3 is a front view of the reagent dropping cover 6 of an automatic staining module for formed components of gynecological secretions according to the present invention, Figure 4 is a left view of the reagent dropping cover 6 of an automatic staining module for formed components of gynecological secretions according to the present invention, Figure 5 is a schematic diagram of the glass slide lifting box 13 of an automatic staining module for formed components of gynecological secretions according to the present invention.

[0030] Please refer to Figure 1, the present invention provides an automatic staining module for formed components of gynecological secretions, including a conveyor belt 1, a steel needle mounting base 2, reagent dropping steel needles 3, set screws 4, a mounting plate 5 for a reagent dropping cover 6, the reagent dropping cover 6, a waste liquid sampling needle 7, a waste liquid sampling needle stopper 8, a spring 9, a glass slide 12, and a glass slide lifting box 13. The conveyor belt 1 is disposed directly below the reagent dropping cover 6. The glass slide lifting box 13 is installed directly below the reagent dropping cover 6. The number of the reagent dropping steel needles is three, and the three reagent dropping steel needles are installed on the steel needle mounting base 2 through the set screws 4. The steel needle mounting base 2 and the reagent dropping cover 6 are installed on the mounting plate 5 of the reagent dropping cover 6, and the three reagent dropping steel needles 3 are inserted into the interior of the reagent dropping cover 6. The waste liquid sampling needle 7 is movably inserted into the steel needle mounting base 2 and the reagent dropping cover 6. The protrusion on the waste liquid sampling needle 7 cooperates with the waste liquid sampling needle stopper 8 installed thereon. The spring 9 is sleeved on the waste liquid sampling needle 7. The conveyor belt 1 can drive the glass slide 12 to be conveyed directly below the reagent dropping cover 6 through a stepping motor; the glass slide lifting box 13 is installed directly below the reagent dropping cover 6 and can perform a linear reciprocating motion up and down through a lead screw stepping motor; the mounting plate 5 of the reagent dropping cover 6 is installed on other fixing mechanisms; the waste liquid sampling needle 7 is inserted into the steel needle mounting base 2 and the reagent dropping cover 6 (movably), and the range of motion of the waste liquid sampling needle 7 is restricted by the protrusion on the waste liquid sampling needle 7 and the waste liquid sampling needle stopper installed thereon. The spring 9 is sleeved on the waste liquid sampling needle 7 to assist the movement of the waste liquid sampling needle 7.

[0031] Please refer to Figure 2 , the reagent dropping cover 6 is provided with an opening inclined surface 10 and a guiding groove 11 for guiding the waste liquid sampling needle 7. The waste liquid sampling needle 7 can move upward a certain distance along the guiding groove 11 when assisted by an external force. When the external force disappears, the waste liquid sampling needle 7 can reset under the action of the spring 9. Two purified water needles are inserted above the inclined surface of the reagent dropping cover 6, and the dyeing needle penetrates through the small hole on the reagent dropping cover 6 to reach the lower side of the inclined surface.

[0032] Please refer to Figure 3 , the bottom of the reagent dropping cover 6 is designed with a bottom skirt 14. When the glass slide 12 is lifted upward, the bottom skirt 14 will be deformed outward, so as to better fit with the glass slide 12 to form a sealed cavity at the lower end. At the same time, the side of the reagent dropping cover 6 is provided with a side baffle 15, which plays a role in guiding and draining water during the instrument debugging process.

[0033] Please refer to Figure 4 and Figure 5, the bottom of the reagent dropping cover 6 is a first inclined surface 16, and the angle of the first inclined surface 16 is 9°. The glass slide lifting box 13 is provided with a second inclined surface 17 having the same inclination angle as the first inclined surface 16. The first inclined surface 16 and the second inclined surface 17 are used in cooperation, so that the lower side of the inner cavity formed by the glass slide 12 and the reagent dropping cover 6 is an inclined surface, enabling the liquid inside to converge to one side of the waste liquid sampling needle 7, facilitating waste discharge.

[0034] Please refer to Figure 6 , Figure 6 is a flowchart of a component automatic staining module and a staining method for gynecological secretions according to the present invention.

[0035] The present invention also provides a staining method for the above-mentioned component automatic staining module for gynecological secretions, which specifically includes the following steps: S1: The glass slide 12 with a sample dried and cured at high temperature is transported by a synchronous belt to directly below the reagent dropping cover 6;

[0036] S2: The glass slide lifting box 13 lifts the glass slide upward. Since the glass slide lifting box 13 has a second inclined surface 17, the glass slide 12 can simultaneously obtain an angle parallel to the first inclined surface 16 of the reagent dropping cover 6. The glass slide lifting box 13 continues to lift, causing the glass slide 12 to contact the reagent dropping cover 6 and deforming the bottom skirt 14 of the reagent dropping cover 6 to form a closed cavity at the bottom composed of the reagent dropping cover 6 and the glass slide 12 that contains the sample. At this time, the waste liquid sampling needle 7 is lifted upward by the glass slide 12, and due to the action of the spring 9, the bottom of the waste liquid sampling needle 7 closely adheres to the glass slide 12;

[0037] S3: Start dropping the dye. Since a closed cavity is formed at the lower side between the glass slide 12 and the reagent dropping cover 6 at this time, drop the dye C and it accumulates in this cavity and finally completely submerges the sample on the glass slide;

[0038] S4: Wait for the dye C to react with the sample; start dropping purified water for rinsing. After the purified water starts to rinse, the pump connected to the waste liquid sampling needle 7 starts to work, sucking away the dye, purified water, and a small part of the sample that has separated from the glass slide 12 in the cavity;

[0039] S5: Subsequently, the glass slide 12 descends with the glass slide lifting box 13 and is taken off the bottom skirt 14 of the reagent dropping cover 6 by about 2 mm, allowing some purified water to overflow from this gap;

[0040] S6: The liquid is drained through the side baffle 15 and flows into the waste liquid storage chamber of the glass slide lifting box 13. Finally, the bottom of the glass slide lifting box 13 pumps away these waste liquids to complete the staining of one sample.

[0041]

[0042] Table 1 Action Timing Diagram of Automatic Staining Method

[0043] In this embodiment, the glass slide 12 with the sample dried and cured at high temperature (the sample is within a circular area with a diameter of about 15 mm) is transported by a synchronous belt to directly below the reagent dropping cover 6. The glass slide lifting box 13 is driven by a lead screw motor to lift the glass slide upward. Since the glass slide lifting box 13 also has an inclined surface, the glass slide 12 can simultaneously obtain an angle parallel to the bottom inclined surface of the reagent dropping cover 6. The glass slide lifting box 13 continues to lift, causing the glass slide 12 to contact the reagent dropping cover 6 and deforming the bottom skirt 14 of the reagent dropping cover 6 (at this time, the bottom skirt 14 does not contact the sample), resulting in a closed cavity at the bottom formed by the reagent dropping cover 6 and the glass slide 12 that contains the sample. At this time, the waste liquid sampling needle 7 is pushed upward by the glass slide 12, and due to the action of the spring 9, the bottom of the waste liquid sampling needle 7 closely adheres to the glass slide 12. According to the timing diagram of the automatic staining method in Table 1, the dye is started to be dropped at this time. Since a closed cavity is formed on the lower side between the glass slide 12 and the reagent dropping cover 6 at this time, it takes 3 s to drop the dye C and accumulate in this cavity until it completely submerges the sample on the glass slide; it takes 9 s to wait for the reaction between the dye C and the sample; the purified water is started to be dropped for rinsing. To save time, the flow rate of the purified water is large and the impact force is large. To reduce the scouring of the sample, the purified water is first sprayed onto the opening inclined surface 10 of the reagent dropping cover 6 and then flows into the lower cavity through the square holes on both sides to rinse the used dye. 1 s after the purified water starts to be rinsed, the pump connected to the waste liquid sampling needle 7 starts to work, sucking away the dye, purified water, and a small part of the sample that has detached from the glass slide 12 in the cavity. Since the liquid in the cavity is in a flowing state while rinsing and dropping the purified water, the cleaning effect can be enhanced; starting from the 23rd s to the 25th s, the glass slide 12 is lowered and removed from the bottom skirt 14 of the reagent dropping cover 6 by about 2 mm as the glass slide lifting box 13 descends (at this time, the bottom surface of the waste liquid sampling needle 7 still closely adheres to the glass slide 12 under the action of the spring 9), causing some purified water to overflow from this gap. Because when the glass slide 12 is joined with the reagent dropping cover 6, it is impossible to be completely sealed, forming an annular dead cavity along the shape of the bottom skirt 14 of the reagent dropping cover 6. The sample cured on the glass slide 12 will inevitably float away from the surface of the glass slide 12 after being soaked by the dye and scoured by the purified water. Part of it will be sucked away by the waste liquid sampling needle 7, and part of it will enter the above-mentioned dead cavity. At this time, rinsing in the above way can avoid cross-contamination, and the rinsing ends at the 33rd s; due to the appearance of the gap, the liquid will inevitably overflow and cannot be completely sucked away by the waste liquid sampling needle 7. At this time, since the gap is small and the liquid flow rate is not fast, a small side baffle 15 of the reagent dropping cover 6 can provide a drainage effect for the liquid, allowing it to flow along the inclined glass slide 12 into the waste liquid storage chamber of the glass slide lifting box 13. From the 34th s to the 36th s, these waste liquids are pumped away from the bottom of the glass slide lifting box 13. Above, after completing all the actions in the entire timing diagram, the staining of one sample is completed, which takes a total of 36 s. The actions are simple, the time used is short, and it is convenient for the realization of automation.

[0044] An automatic staining module and staining method for formed components of gynecological secretions according to the present invention are simple in operation and short in time consumption during operation, facilitating the realization of rapid automation. The designed action timing diagram has the originality of "staining once and cleaning once". The slide 12 itself and the reagent dropping cover 6 together form a cavity sealed at the lower end, enabling the sample to be soaked in the dye inside and facilitating the cleaning of the reagent dropping cover 6 after separation. The inner side of the reagent dropping cover 6 is separated into upper and lower layers by a perforated inclined surface. The dye needle penetrates through the small hole into the lower layer, approaching the sample area, reducing the scouring of the sample caused by the dripping of the dye. The purified water needle is in the upper layer, which can increase the flow rate and velocity. Due to the buffering of the partition layer, it will not cause excessive scouring to the sample. The bottom of the reagent dropping cover 6 is designed with a soft bottom skirt 14, and at the same time, it is inclined, facilitating sealing after contacting the slide 12 and the convergence of the liquid inside to one side of the waste liquid sampling needle 7. The waste liquid sampling needle 7 of the reagent dropping cover 6 sub-module can always fit with the slide 12 with the assistance of the spring 9 when the slide 12 rises, facilitating the extraction of waste liquid. The slide lifting box 13 has an inclined surface with the same inclined surface angle as the bottom skirt 14 of the reagent dropping cover 6, which can make the slide 12 parallel to the inclined surface of the bottom skirt 14 of the reagent dropping cover 6 while lifting the slide 12.

[0045] The above disclosure is only one or more preferred embodiments of the present application, and the scope of rights of the present application cannot be limited thereby. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present application still fall within the scope covered by the present application.

Claims

1. An automatic staining module for formed components of gynecological secretions, characterized in that it includes a conveyor belt, a steel needle mounting seat, reagent dropping steel needles, set screws, a reagent dropping cover mounting plate, a reagent dropping cover, a waste liquid sampling needle, a waste liquid sampling needle baffle, a spring, a glass slide, and a glass slide lifting box. The conveyor belt is arranged directly below the reagent dropping cover. The glass slide lifting box is installed directly below the reagent dropping cover. The number of reagent dropping steel needles is three. The three reagent dropping steel needles are installed on the steel needle mounting seat through the set screws. The steel needle mounting seat and the reagent dropping cover are installed on the reagent dropping cover mounting plate, and the three reagent dropping steel needles are inserted into the interior of the reagent dropping cover. The waste liquid sampling needle is movably inserted into the steel needle mounting seat and the reagent dropping cover. The protrusion on the waste liquid sampling needle cooperates with the waste liquid sampling needle baffle. The spring is sleeved on the waste liquid sampling needle. The bottom of the reagent dropping cover is provided with a deformable bottom skirt.

2. The automatic staining module for formed components of gynecological secretions according to claim 1, characterized in that the reagent dropping cover is provided with an opening inclined surface and a guide groove.

3. The automatic staining module for formed components of gynecological secretions according to claim 2, characterized in that the two sides of the reagent dropping cover are provided with side baffles.

4. The automatic staining module for formed components of gynecological secretions according to claim 3, characterized in that the bottom of the reagent dropping cover is a first inclined surface, and the angle of the first inclined surface is 9°.

5. The automatic staining module for formed components of gynecological secretions according to claim 4, characterized in that the glass slide lifting box is provided with a second inclined surface having the same inclination angle as the first inclined surface.

6. A staining method for the formed component automatic staining module of gynecological secretions as described in claim 5, characterized in that, Specifically, it includes the following steps: The glass slide with the sample dried and cured at high temperature is transported by the synchronous belt to directly below the reagent dropping cover; The glass slide lifting box lifts the glass slide upward. Since the glass slide lifting box has a second inclined surface, the glass slide can simultaneously obtain an angle parallel to the first inclined surface of the reagent dropping cover. The glass slide lifting box continues to lift, causing the glass slide to contact the reagent dropping cover and deforming the bottom skirt of the reagent dropping cover, resulting in a closed cavity at the bottom formed by the reagent dropping cover and the glass slide that contains the sample. At this time, the waste liquid sampling needle is pushed upward by the glass slide. Due to the action of the spring, the bottom of the waste liquid sampling needle closely adheres to the glass slide; Start dropping the dye. Since a closed cavity is formed between the glass slide and the reagent dropping cover at the lower side at this time, drop the dye C and it accumulates in this cavity and finally completely immerses the sample on the glass slide; Wait for the dye C to react with the sample; start dropping purified water for rinsing. After the purified water starts to rinse, the pump connected to the waste liquid sampling needle starts to work, sucking away the dye, purified water, and a small part of the sample that has separated from the glass slide in the cavity; Subsequently, the glass slide descends as the glass slide lifting box descends, and separates from the bottom skirt of the reagent dropping cover by 2 mm, causing some purified water to overflow from the gap. The liquid is drained through the side baffle and flows into the waste liquid storage chamber of the slide lifting box. Finally, the bottom of the slide lifting box pumps away these waste liquids to complete the staining of a sample.

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