Microbial activated coal gangue powder, soil conditioner based on microbial activation, preparation method and application thereof

Abstract

The present application relates to the technical field of resource utilization of coal-based solid waste, and in particular to a microbially activated coal gangue powder, a soil conditioner, a preparation method and an application thereof based on microbial activation; the raw materials of the microbially activated coal gangue powder include: coal gangue powder, livestock and poultry manure powder, plant fiber powder, a composite bacterial agent, and the balance is water; wherein the composite bacterial agent is a mixture of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus; the method includes: drying the coal gangue, livestock and poultry manure and plant fiber respectively, and then crushing and grinding them to obtain coal gangue powder, livestock and poultry manure powder and plant fiber powder respectively; mixing the coal gangue powder, livestock and poultry manure powder, plant fiber powder and water, and adding the composite bacterial agent, and then aerobically fermenting to obtain the microbially activated coal gangue powder; by adding the composite bacterial agent, livestock and poultry manure powder and plant fiber powder to the coal gangue powder, the decomposition and conversion efficiency of organic matter can be improved on the basis of efficient dissociation and release of inorganic nutrient elements.

Description

Technical Field

[0001] The present application relates to the technical field of resource utilization of coal-based solid waste, and in particular to a microbially activated coal gangue powder and soil conditioner based on microbial activation, as well as a preparation method and application thereof. Background Art

[0002] Coal gangue is the waste generated during coal mining and processing, and is one of the largest industrial solid wastes currently discharged. The accumulation of large amounts of coal gangue will pollute the soil, groundwater and atmosphere (gangue naturally produces harmful gases) on the one hand, and will occupy a large area of ​​land on the other. Therefore, it is necessary to reduce the amount of coal gangue and utilize it as a resource.

[0003] Since gangue comes from the strata, its composition is similar to that of soil. It is rich in organic matter, inorganic nutrients and trace elements. Therefore, applying gangue to ecological planting is undoubtedly one of the best ways to dispose of it. However, the organic components in gangue are usually highly solidified and difficult to decompose and transform. At the same time, the inorganic nutrients such as nitrogen, phosphorus and potassium and trace elements such as boron, molybdenum, zinc, manganese, iron and copper contained in it are highly combined with minerals and are difficult to dissolve and release for plant absorption and utilization, thereby increasing the technical difficulty of the ecological application of gangue. In recent years, the preparation of gangue-based biological organic fertilizers by microbial activation has received widespread attention. Under the action of microbial fermentation, the nutrients in the gangue can be efficiently dissociated and released for plant absorption and utilization. However, at this stage, most of the microbial fermentation technologies are aimed at the dissociation and release of inorganic nutrients in gangue, while the decomposition and transformation technology of organic matter inside it is relatively scarce. Therefore, how to provide a microbial activated coal gangue powder based on microbial activation to achieve not only high dissociation and release of inorganic nutrient elements in coal gangue but also high decomposition and conversion efficiency of organic matter is a technical problem that urgently needs to be solved. Summary of the invention

[0004] The present application provides a microbially activated coal gangue powder, a soil conditioner and a preparation method based on microbial activation, so as to solve the problem that the microbially activated coal gangue powder in the prior art is difficult to improve the decomposition and conversion efficiency of organic matter on the basis of efficient dissociation and release of inorganic nutrient elements.

[0005] In a first aspect, the present application provides a microbial activated coal gangue powder based on microbial activation, wherein the raw materials of the microbial activated coal gangue powder include, by mass percentage:

[0006] Gangue powder: 35%~40%, livestock manure powder: 22%~30%, plant fiber powder: 8%~12%, compound bacterial agent: 1%~2%, and the balance is water;

[0007] Wherein, the composite bacterial agent is a mixture of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus.

[0008] Optionally, the mass ratio of the Acinetobacter luffi, the Bacillus mucilaginosus and the Bacillus cereus is 1 to 3:1:1.

[0009] Optionally, the viable counts of the Acinetobacter luffii, the Bacillus mucilaginosus and the Bacillus cereus are respectively greater than 1*10 9 cfu / mL.

[0010] Optionally, the Acinetobacter lwoffii is isolated from coal gangue, and the Acinetobacter lwoffii has been deposited in the China Center for Type Culture Collection on September 15, 2022, with the deposit number CCTCC NO. M20221438.

[0011] Optionally, the livestock manure powder includes at least one of sheep manure, chicken manure and pig manure; and / or,

[0012] The plant fiber powder includes at least one of straw powder, forage powder, leaf powder and wheat bran.

[0013] In a second aspect, the present application provides a method for preparing the microbial activated coal gangue powder according to the first aspect, the method comprising:

[0014] The coal gangue, livestock excrement and plant fiber are dried respectively, and then crushed and ground to obtain coal gangue powder, livestock excrement powder and plant fiber powder respectively;

[0015] The gangue powder, the livestock excrement powder, the plant fiber powder and water are mixed, and the composite bacterial agent is added, followed by aerobic fermentation to obtain microbially activated gangue powder.

[0016] In a third aspect, an embodiment of the present application provides a soil conditioner based on microbial activation, wherein the raw materials of the soil conditioner include the microbially activated coal gangue powder described in the first aspect, and the mass percentage of the microbially activated coal gangue powder is 88% to 93%.

[0017] Optionally, the raw materials of the soil conditioner may further include, by mass percentage:

[0018] Mineral adsorbent: 5% to 8%, nutritional regulator: 1% to 2% and plant probiotics: 1% to 2%;

[0019] Wherein, the mineral adsorbent includes bentonite and / or zeolite powder;

[0020] The nutrient regulator includes at least one of compound fertilizer, urea, phosphate fertilizer and potash fertilizer;

[0021] The plant probiotics include at least one of Bacillus subtilis, Trichoderma harzianum and Rhizobium.

[0022] In a fourth aspect, the present application provides a method for preparing the soil conditioner according to the third aspect, the method comprising:

[0023] The microbial activated coal gangue powder, the mineral adsorbent, the nutrient regulator and the plant probiotics are mixed to obtain a soil conditioner.

[0024] In the fifth aspect, an embodiment of the present application provides an application of a soil conditioner based on microbial activation, the application comprising: using the soil conditioner described in the third aspect in the ecological restoration of sandy soil, saline-alkali land, landscaping, mines and / or slopes, and the amount of the soil conditioner used is 500kg / mu to 800kg / mu.

[0025] The above technical solution provided by the embodiment of the present application has the following advantages compared with the prior art:

[0026] The present application provides a microbial activated coal gangue powder based on microbial activation. A composite bacterial agent is added to the coal gangue powder, and the composite bacterial agent is used to ferment and activate the coal gangue powder. At the same time, poultry and livestock manure powder and plant fiber powder are added as auxiliary materials to provide a more friendly environment for the reproduction of the composite bacterial agent. The composite bacterial agent uses Acinetobacter luffii as the main bacterial agent to decompose and convert the minerals in the coal gangue powder into humic acid that can be absorbed and utilized by plants. At the same time, Bacillus colloidus can achieve efficient inorganic nutrient elements such as potassium, silicon, calcium, and sulfur in the coal gangue powder. Dissociation: Bacillus cereus can decompose cellulose, hemicellulose and plant protein in poultry and livestock manure powder and plant fiber powder in auxiliary materials, and convert them into beneficial substances such as monosaccharides and amino acids for the growth and utilization of other microorganisms, thereby increasing the organic matter and nutrients in the mixed materials, allowing the various strains in the composite bacterial agent to grow synergistically with each other, further improving the decomposition and conversion of organic matter in coal gangue powder, and making the coal gangue powder activated by microorganisms have good fertilizer efficiency, thereby achieving the improvement of the decomposition and conversion efficiency of organic matter on the basis of efficient dissociation and release of inorganic nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0028] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0029] Figure 1 A schematic diagram of a process for preparing microbial activated coal gangue powder provided in an embodiment of the present application;

[0030] Figure 2 A schematic diagram of a process for preparing a soil conditioner provided in an embodiment of the present application;

[0031] Figure 3 A graph showing the comparison of the growth curves of the composite bacterial agent and the single Acinetobacter lüfyi in coal gangue culture medium provided in the examples of the present application;

[0032] Figure 4 A physical picture of the soil conditioner provided in the embodiments of the present application;

[0033] Figure 5 This is a graph showing the results of applying the soil conditioner provided in an embodiment of the present application to planting in sandy soil. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0035] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in this application can be purchased from the market or prepared by existing methods.

[0036] The creative thinking of this application is:

[0037] At present, most of the microbial fermentation technologies are aimed at the dissociation of inorganic nutrients in coal gangue. For example, using Stenotrophomonas maltophilia GZU-Stm01, Bacillus fusiformis GZULys01 and Aspergillus fumigatus GZT-Asp01 to activate single coal gangue, although it can achieve efficient dissociation of inorganic elements such as phosphorus, potassium, silicon, sulfur, calcium in coal gangue, but the decomposition effect of organic components in coal gangue is poor. Therefore, how to provide a microbial activated coal gangue powder based on microbial activation to achieve not only the dissociation and release of inorganic nutrients in coal gangue but also the decomposition and transformation of organic matter is a technical problem that needs to be solved urgently.

[0038] like Figure 1 As shown, the embodiment of the present application provides a microbial activated coal gangue powder based on microbial activation. In terms of mass percentage, the raw materials of the microbial activated coal gangue powder include:

[0039] Gangue powder: 35%~40%, livestock manure powder: 22%~30%, plant fiber powder: 8%~12%, compound bacterial agent: 1%~2%, and the balance is water;

[0040] The composite bacterial agent is a mixture of Acinetobacter lwoffii XAQ2297, Bacillus mucilaginosus Krassilnikov and Bacillus cereus.

[0041] In the embodiment of the present application, the positive effect of limiting the mass percentage of coal gangue powder to 35% to 40% is that within this mass percentage range, sufficient coal gangue powder can be ensured, so that the composite bacterial agent can improve the decomposition and conversion efficiency of organic matter on the basis of efficient dissociation and release of inorganic nutrient elements.

[0042] The positive effect of the mass percentage of livestock and poultry manure powder being 22% to 30% is that within this mass percentage range, the Bacillus cereus in the composite bacterial agent can decompose the cellulose, hemicellulose and plant protein in the livestock and poultry manure powder, thereby converting them into monosaccharides, amino acids and other beneficial substances for the growth and utilization of other microorganisms, while increasing the organic matter and nutrients in the mixed material.

[0043] The positive effect of the mass percentage of plant fiber powder being 8% to 12% is that within this mass percentage range, the Bacillus cereus in the composite bacterial agent can decompose the cellulose, hemicellulose and plant protein in the plant fiber powder, thereby converting them into monosaccharides, amino acids and other beneficial substances for the growth and utilization of other microorganisms.

[0044] The positive effect of the composite bacterial agent with a mass percentage of 1% to 2% is that within the range of this mass percentage, the Acinetobacter luffi in the composite bacterial agent can decompose and convert the minerals in the coal gangue powder into humic acid that can be absorbed and utilized by plants, while the Bacillus subtilis can achieve efficient dissociation of inorganic nutrients such as potassium, silicon, calcium, and sulfur in the coal gangue powder. Bacillus cereus can decompose cellulose, hemicellulose, and plant protein in the poultry and livestock manure powder and plant fiber powder in the auxiliary materials, and convert them into monosaccharides, amino acids and other beneficial substances for the growth and utilization of other microorganisms, thereby increasing the organic matter and nutrients in the mixed materials, allowing the various bacterial species in the composite bacterial agent to grow synergistically with each other, thereby improving the decomposition and conversion efficiency of organic matter on the basis of efficient dissociation and release of inorganic nutrients.

[0045] In some optional embodiments, the mass ratio of the Acinetobacter lwoffii XAQ2297, the Bacillus mucilaginosus Krassilnikov and the Bacillus cereus is 1-3:1:1.

[0046] In the embodiments of the present application, the positive effect of limiting the volume ratio of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus is that the synergistic growth of the various bacterial species in the composite bacterial agent can be ensured within the range of the volume ratio, thereby achieving the improvement of the decomposition and conversion efficiency of organic matter on the basis of the efficient dissociation and release of inorganic nutrient elements.

[0047] In some optional embodiments, the viable counts of Acinetobacter lwoffii XAQ2297, Bacillus mucilaginosus Krassilnikov and Bacillus cereus are respectively greater than 1*10 9 cfu / mL.

[0048] In the examples of the present application, the number of live bacteria of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus is limited to further ensure the synergistic growth of the various strains in the composite bacterial agent, and to ensure that the various strains have a complete effect on the coal gangue powder, livestock manure powder and plant fiber powder, thereby improving the decomposition and conversion efficiency of organic matter on the basis of efficient dissociation and release of inorganic nutrients.

[0049] In some optional embodiments, the Acinetobacter lwoffii is isolated from coal gangue, and the Acinetobacter lwoffii has been deposited in the China Center for Type Culture Collection on September 15, 2022, with the deposit number CCTCC NO. M20221438.

[0050] In the examples of the present application, the specific source of Acinetobacter luffii is defined and the corresponding accession number is provided, so that Acinetobacter luffii can efficiently decompose organic minerals in coal gangue powder and convert them into humic acid that can be absorbed and utilized by plants.

[0051] In some optional embodiments, the livestock manure powder includes at least one of sheep manure, chicken manure and pig manure; and / or,

[0052] The plant fiber powder includes at least one of straw powder, forage powder, leaf powder and wheat bran.

[0053] In the embodiments of the present application, the specific types of livestock manure powder and plant fiber are limited to include most common livestock manure powder and plant fiber powder, thereby increasing the source of microbial activated coal gangue of the present application and making the raw material source of microbial activated coal gangue of the present application universal.

[0054] The poultry and livestock manure powder can be sheep manure, chicken manure, pig manure, a mixture of sheep manure and chicken manure, or a mixture of sheep manure, chicken manure and pig manure.

[0055] The plant fiber powder can be straw powder, grass powder, leaf powder, wheat bran, a mixture of straw powder, grass powder and leaf powder, or a mixture of grass powder, leaf powder and wheat bran.

[0056] Based on a general inventive concept, the present application provides a method for preparing the microbial activated coal gangue powder, the method comprising:

[0057] S1. The gangue, livestock manure and plant fiber are dried, crushed and ground to obtain gangue powder, livestock manure powder and plant fiber powder, respectively;

[0058] S2. Mix the gangue powder, the livestock manure powder, the plant fiber powder and water, add the composite bacterial agent, and then perform aerobic fermentation to obtain microbially activated gangue powder.

[0059] In the embodiment of the present application, by first drying the raw materials and then crushing and grinding them, it can be ensured that the particle size of the raw materials meets the requirements, and it can also ensure that the subsequent composite bacterial agent decomposes and transforms the organic matter in the coal gangue powder, livestock manure powder and plant fiber powder, and dissociates the inorganic nutrients in the coal gangue powder.

[0060] The total organic matter content of the coal gangue powder formed by crushing and grinding is ≥30%, and its particle size is <35 mesh.

[0061] The particle size of the livestock and poultry manure powder formed by crushing and grinding is less than 10 mesh.

[0062] The particle size of the plant fiber powder formed by crushing and grinding is less than 10 meshes.

[0063] This method is for the preparation of the above-mentioned microbial activated coal gangue powder. The specific composition and ratio of the microbial activated coal gangue powder can refer to the above-mentioned embodiment. Since this method adopts part or all of the technical solutions of the above-mentioned embodiment, it has at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiment, which will not be repeated here one by one.

[0064] In some optional embodiments, the temperature of the aerobic fermentation is 30° C. to 40° C., the duration of the aerobic fermentation is 7 to 10 days, the method of the aerobic fermentation is indoor composting, and the material is turned over every 3 days during the aerobic fermentation process.

[0065] In the embodiments of the present application, specific parameters and methods of aerobic fermentation are defined so that each bacterial species in the composite bacterial agent can multiply in large quantities, and can efficiently dissociate and release inorganic nutrients in coal gangue powder and effectively decompose and transform organic matter.

[0066] Based on a general inventive concept, an embodiment of the present application provides a soil conditioner based on microbial activation, wherein the raw materials of the soil conditioner include the microbially activated coal gangue powder, and the mass percentage of the microbially activated coal gangue powder is 88% to 93%.

[0067] In the embodiment of the present application, the positive effect of limiting the mass percentage of microbially activated coal gangue powder to 88% to 93% is that within this mass percentage range, it can not only ensure that the nutrients in the improver are rich and sufficient, but also reduce costs.

[0068] The soil conditioner is realized based on the above-mentioned microbial activated coal gangue powder. The specific composition and proportion of the microbial activated coal gangue powder can refer to the above-mentioned embodiment. Since the method adopts part or all of the technical solutions of the above-mentioned embodiment, it has at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiment, which will not be repeated here one by one.

[0069] In some optional embodiments, the raw materials of the soil conditioner further include, by mass percentage:

[0070] Mineral adsorbent: 5% to 8%, nutritional regulator: 1% to 2% and plant probiotics: 1% to 2%;

[0071] Wherein, the mineral adsorbent includes bentonite and / or zeolite powder;

[0072] The nutrient regulator includes at least one of compound fertilizer, urea, phosphate fertilizer and potash fertilizer;

[0073] The plant probiotics include at least one of Bacillus subtilis, Trichoderma harzianum and Rhizobium.

[0074] In the embodiment of the present application, the positive effect of limiting the mass percentage of the mineral adsorbent to 5% to 8% is that within this mass percentage range, the mineral adsorbent can fully retain the nutrients in the soil conditioner and improve the water and fertilizer retention properties of the conditioner.

[0075] The positive effect of limiting the mass percentage of plant probiotics to 1% to 2% is that within this mass percentage range, it can ensure that the soil conditioner contains sufficient plant probiotics, which is beneficial to the germination and growth of plants and has the effect of promoting rooting and growth.

[0076] The mineral adsorbent may be bentonite or zeolite powder;

[0077] The nutrient regulator can be compound fertilizer, urea, phosphate fertilizer, potash fertilizer, or a mixture of compound fertilizer, phosphate fertilizer and potash fertilizer;

[0078] The plant probiotics may be Bacillus subtilis, Trichoderma harzianum, Rhizobium, a mixed strain of Bacillus subtilis and Trichoderma harzianum, a mixed strain of Trichoderma harzianum and Rhizobium, a mixed strain of Bacillus subtilis and Rhizobium, or a mixed strain of Bacillus subtilis, Trichoderma harzianum and Rhizobium.

[0079] like Figure 2 As shown, based on a general inventive concept, the present application embodiment provides a method for preparing the soil conditioner, the method comprising:

[0080] S1. Mixing the microbial activated coal gangue powder, the mineral adsorbent, the nutrient regulator and the plant probiotics to obtain a soil conditioner.

[0081] This method is for the preparation of the above-mentioned soil conditioner. The specific composition and ratio of the soil conditioner can refer to the above-mentioned embodiment. Since this method adopts part or all of the technical solutions of the above-mentioned embodiment, it at least has all the beneficial effects brought by the technical solutions of the above-mentioned embodiment, which will not be described one by one here.

[0082] Based on a general inventive concept, an embodiment of the present application provides an application of a soil conditioner based on microbial activation, the application comprising: using the soil conditioner in the ecological restoration of sandy soil, saline-alkali land, landscaping, mines and / or slopes, and the amount of the soil conditioner used is 500kg / mu to 800kg / mu.

[0083] In the embodiments of the present application, the positive effect of limiting the dosage of the soil conditioner to 500kg / mu to 800kg / mu is that within the range of the dosage, sufficient microbial activated coal gangue powder in the soil conditioner can be ensured, thereby enabling the ecological restoration of sandy land, saline-alkali land, landscaping and mine slopes to be achieved through the soil conditioner.

[0084] This application is realized based on the above-mentioned soil conditioner. The specific composition and proportion of the soil conditioner can refer to the above-mentioned embodiment. Since this application adopts part or all of the technical solutions of the above-mentioned embodiment, it has at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiment, which will not be described one by one here.

[0085] The present application will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are intended only to illustrate the present application and are not intended to limit the scope of the present application. The experimental methods for which specific conditions are not specified in the following examples are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0086] Example 1

[0087] Coal gangue from the Huangbaiczi mining area in Wuhai City, Inner Mongolia was selected as the main raw material. Its total organic matter content was 32.4%, and all kinds of heavy metal elements contained were within the standard. At the same time, corn straw was selected as plant fiber and sheep manure as livestock manure as auxiliary materials, and soil conditioner preparation and field application tests were carried out in Wuhai City.

[0088] A microbial activated coal gangue powder based on microbial activation, wherein the raw materials of the microbial activated coal gangue powder include, by mass percentage:

[0089] Gangue powder: 35%, livestock manure powder: 25%, plant fiber powder: 10%, compound bacterial agent: 2%, tap water: 28%;

[0090] Among them, the composite bacterial agent is a mixture of Acinetobacter luffi, Bacillus subtilis and Bacillus cereus.

[0091] The volume ratio of Acinetobacter luffi, Bacillus mucilaginosus and Bacillus cereus is 2:1:1.

[0092] The viable counts of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus were >1*10 9 cfu / mL.

[0093] Acinetobacter lwoffii was isolated from coal gangue. Acinetobacter lwoffii was deposited in the China Center for Type Culture Collection on September 15, 2022, with the deposit number CCTCC NO. M20221438.

[0094] The livestock manure powder is sheep manure; the plant fiber powder is straw powder.

[0095] A method for preparing microbial activated coal gangue powder, comprising:

[0096] S1. The gangue, livestock manure and plant fiber are dried, crushed and ground to obtain gangue powder, livestock manure powder and plant fiber powder, respectively;

[0097] S2. Mix coal gangue powder, livestock manure powder, plant fiber powder and water, add composite bacterial agent, and then perform aerobic fermentation to obtain microbially activated coal gangue powder.

[0098] Among them, the average ambient temperature of aerobic fermentation is 32°C, the duration of aerobic fermentation is 10 days, the method of aerobic fermentation is indoor composting, and the materials are turned over every 3 days during the aerobic fermentation process.

[0099] A soil conditioner based on microbial activation, wherein the raw materials of the soil conditioner include, by mass percentage: 90% of microbial activated coal gangue powder, 6% of mineral adsorbent, 2% of nutrient regulator and 2% of plant probiotics;

[0100] Among them, the mineral adsorbent is bentonite; the nutrient regulator is compound fertilizer; and the plant probiotics are Bacillus subtilis and Trichoderma harzianum.

[0101] A method for preparing the soil conditioner of the third aspect comprises:

[0102] S1. Mix microbially activated coal gangue powder, mineral adsorbents, nutrient regulators and plant probiotics to obtain a soil conditioner.

[0103] Calculated on a drying basis, the total organic matter content of the prepared soil conditioner is 42.31%, the total effective nutrient content of the soil conditioner is 1.63%, the pH value of the soil conditioner is 6.8, and the moisture content (mass ratio) of the soil conditioner is 12.35%.

[0104] Example 2

[0105] Comparing Example 2 with Example 1, the difference between Example 2 and Example 1 is:

[0106] In terms of mass percentage, the raw materials for microbial activation of coal gangue powder include:

[0107] Gangue powder: 40%, livestock manure powder: 22%, plant fiber powder: 10%, compound bacterial agent: 2%, tap water: 28%;

[0108] Among them, the composite bacterial agent is a mixture of Acinetobacter luffi, Bacillus subtilis and Bacillus cereus.

[0109] In terms of mass percentage, the raw materials of the soil conditioner include: 90% microbial activated coal gangue powder, 6% mineral adsorbent, 2% nutrient regulator and 2% plant probiotics;

[0110] Among them, the mineral adsorbent is bentonite; the nutrient regulator is compound fertilizer; and the plant probiotics are Bacillus subtilis and Trichoderma harzianum.

[0111] On a drying basis, the total organic matter content of the prepared soil conditioner is 40.5%, the total effective nutrient content of the soil conditioner is 1.52%, the pH value of the soil conditioner is 6.91, and the moisture content (mass ratio) of the soil conditioner is 13.11%.

[0112] Example 3

[0113] Comparing Example 3 with Example 1, the difference between Example 3 and Example 1 is:

[0114] In terms of mass percentage, the raw materials for microbial activation of coal gangue powder include:

[0115] Coal gangue powder: 38%, livestock manure powder: 30%, plant fiber powder: 8%, compound bacterial agent: 1%, tap water: 23%;

[0116] Among them, the composite bacterial agent is a mixture of Acinetobacter luffi, Bacillus subtilis and Bacillus cereus.

[0117] The volume ratio of Acinetobacter luffi, Bacillus mucilaginosus and Bacillus cereus is 1:1:1

[0118] In terms of mass percentage, the raw materials of the soil conditioner include: 93% microbial activated coal gangue powder, 8% mineral adsorbent, 2% nutrient regulator and 1% plant probiotics;

[0119] Example 4

[0120] Comparing Example 4 with Example 1, the difference between Example 4 and Example 1 is:

[0121] In terms of mass percentage, the raw materials for microbial activation of coal gangue powder include:

[0122] Gangue powder: 40%, livestock manure powder: 22%, plant fiber powder: 12%, compound bacterial agent: 2%, tap water: 24%;

[0123] Among them, the composite bacterial agent is a mixture of Acinetobacter luffi, Bacillus subtilis and Bacillus cereus.

[0124] The volume ratio of Acinetobacter luffi, Bacillus mucilaginosus and Bacillus cereus is 3:1:1

[0125] In terms of mass percentage, the raw materials of the soil conditioner include: microbial activated coal gangue powder 90%, mineral adsorbent: 7%, nutrient regulator: 1% and plant probiotics: 2%;

[0126] Comparative Example 1

[0127] Comparative Example 1 and Example 1 are compared. The difference between Comparative Example 1 and Example 1 is:

[0128] No compound bacterial agent was added.

[0129] On a drying basis, the total organic matter content of the prepared soil conditioner is 42.5%, the total effective nutrient content of the soil conditioner is 0.62%, the pH value of the soil conditioner is 7.21, and the moisture content (mass ratio) of the soil conditioner is 15.11%.

[0130] Comparative Example 2

[0131] Comparative Example 2 is compared with Example 1. The difference between Comparative Example 2 and Example 1 is:

[0132] The raw materials of microbial activated coal gangue powder include: coal gangue powder: 50%, livestock manure powder: 20%, plant fiber powder: 5%, compound bacterial agent: 5%, and the balance is water.

[0133] The raw materials of the soil conditioner include: microbial activated coal gangue powder: 95%, mineral adsorbent: 3%, nutrient regulator: 1% and plant probiotics: 1%.

[0134] Comparative Example 3

[0135] Comparative Example 3 and Example 1 are compared. The difference between Comparative Example 3 and Example 1 is:

[0136] The raw materials of microbial activated coal gangue powder include: coal gangue powder: 30%, livestock manure powder: 35%, plant fiber powder: 15%, compound bacterial agent: 5%, and the balance is water.

[0137] The raw materials of the soil conditioner include: microbial activated coal gangue powder: 85%, mineral adsorbent: 10%, nutrient regulator: 3% and plant probiotics: 2%.

[0138] Related experiments and effect data:

[0139] The nutrient indexes in the soil conditioners obtained in Example 1, Example 2 and Comparative Example 1 were detected, and each soil conditioner was used to improve the Yellow River sediment at an application rate of 500 kg / mu. At the same time, oat crops were planted on the improved Yellow River sediment to evaluate the effect. The results are shown in Table 1. Figure 4 and Figure 5 shown.

[0140] Table 1 Nutrient content index and effect evaluation of the soil conditioner obtained in Example 1, Example 2 and Comparative Example 1

[0141]

[0142] Detailed analysis of Table 1:

[0143] As can be seen from Table 1, the microbial activated coal gangue powder of the present application can effectively increase the content of effective nitrogen, phosphorus, potassium, silicon, sulfur and other plant nutrient elements in the coal gangue powder, among which the ammonium nitrogen content can be increased by more than 10 times (more than 2 times compared with comparative example 1), the effective phosphorus content can be increased by 600 to 700 times (more than 3 times compared with comparative example 1), and the available potassium content can be increased by more than 50 times (about 5 times compared with comparative example 1); the effective silicon content can be increased by more than 10 times.

[0144] And through field planting experiments, it was found that Figure 4 and Figure 5 As shown in the figure, when soil conditioners are applied to oat planting in the Yellow River sandy land, the growth rate of crops can be significantly increased and the nutritional balance in the crops can be adjusted. Therefore, they can be widely used in sandy soil improvement, agricultural and forestry planting and mine ecological restoration projects.

[0145] One or more technical solutions in the embodiments of the present application also have at least the following technical effects or advantages:

[0146] (1) The microbial activated coal gangue powder based on microbial activation provided in the embodiment of the present application achieves efficient dissociation of organic and inorganic components in coal gangue by adding a composite bacterial agent mainly composed of Acinetobacter lwoffii XAQ2297 for fermentation activation, thereby preparing nutrient-rich microbial activated coal gangue powder, and then preparing a low-cost, high-fertilizer-efficiency, and significant plant growth-promoting coal gangue-based soil conditioner by compounding with functional materials. The coal gangue-based soil conditioner can be widely used in sandy land, saline-alkali land improvement, landscaping and mine slope ecological restoration projects, so as to realize large-scale resource utilization of coal gangue.

[0147] (2) A microbial activated coal gangue powder based on microbial activation provided in the embodiments of the present application, such as Figure 3 As shown, the selected composite bacterial agent can grow and multiply rapidly in the coal gangue-based culture medium, showing a good synergistic effect.

[0148] (3) A soil conditioner based on microbial activation provided in the embodiments of the present application, such as Figure 4 and Figure 5 As shown, after the Yellow River silt was improved with soil conditioners, the growth rate of oat crops was significantly accelerated, the crop roots and stems became thicker, and the yield increased.

[0149] Various embodiments of the present application may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present application; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0150] In the present application, in the absence of any contrary description, the directional words used, such as "upper" and "lower", are specifically the directions of the drawings in the accompanying drawings. In addition, in the description of the present specification, the terms "including", "comprising", etc. refer to "including but not limited to". In this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. In this article, "and / or" describes the association relationship of the associated objects, indicating that there may be three relationships, for example, A and / or B, which can represent: A exists alone, A and B exist at the same time, and B exists alone. Wherein A, B can be singular or plural. In this article, "at least one" refers to one or more, and "plural" refers to two or more. "At least one", "at least one of the following" or similar expressions refer to any combination of these items, including any combination of single items (individuals) or plural items (individuals). For example, "at least one of a, b, or c", or "at least one of a, b and c", can both mean: a, b, c, ab (i.e. a and b), ac, bc, or abc, where a, b, c can be single or plural, respectively.

[0151] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novel features applied for herein.

Claims

1. A soil conditioner based on microbial activation, characterized in that: The raw materials of the soil conditioner include microbial activated coal gangue powder, and the mass percentage of the microbial activated coal gangue powder is 88% to 93%; in terms of mass percentage, the raw materials of the microbial activated coal gangue powder include: Gangue powder: 35%~40%, livestock manure powder: 22%~30%, plant fiber powder: 8%~12%, compound bacterial agent: 1%~2%, and the balance is water; Wherein, the composite bacterial agent is a mixture of Acinetobacter luffii, Bacillus mucilaginosus and Bacillus cereus; The mass ratio of the Acinetobacter luffii, the Bacillus colloids and the Bacillus cereus is 1 to 3:1:1; the viable counts of the Acinetobacter luffii, the Bacillus colloids and the Bacillus cereus are respectively greater than 1*10 9 cfu / mL; In terms of mass percentage, the raw materials of the soil conditioner also include: Mineral adsorbent: 5% to 8%, nutritional regulator: 1% to 2% and plant probiotics: 1% to 2%; Wherein, the mineral adsorbent includes bentonite and / or zeolite powder; The nutrient regulator includes at least one of compound fertilizer, urea, phosphate fertilizer and potash fertilizer; The plant probiotics include at least one of Bacillus subtilis, Trichoderma harzianum and Rhizobium.

2. The soil conditioner according to claim 1, characterized in that The Acinetobacter lwoffii was isolated from coal gangue, and the Acinetobacter lwoffii was deposited in the China Center for Type Culture Collection on September 15, 2022, with the deposit number CCTCC NO. M20221438.

3. The soil conditioner according to claim 1, characterized in that The livestock manure powder includes at least one of sheep manure, chicken manure and pig manure; and / or, The plant fiber powder includes at least one of straw powder, forage powder, leaf powder and wheat bran.

4. The soil conditioner according to claim 1, characterized in that The method for preparing microbial activated coal gangue powder comprises: The gangue, livestock excrement and plant fiber are dried respectively, and then crushed and ground to obtain gangue powder, livestock excrement powder and plant fiber powder respectively; The gangue powder, the livestock excrement powder, the plant fiber powder and water are mixed, and the composite bacterial agent is added, followed by aerobic fermentation to obtain microbially activated gangue powder.

5. A method for preparing the soil conditioner according to any one of claims 1 to 4, characterized in that: The method comprises: The microbial activated coal gangue powder, the mineral adsorbent, the nutrient regulator and the plant probiotics are mixed to obtain a soil conditioner.

6. An application of a soil conditioner based on microbial activation, characterized in that: The application includes: using the soil conditioner as described in any one of claims 1 to 4 in the ecological restoration of sandy soil, saline-alkali land, landscaping, mines and / or slopes, and the amount of the soil conditioner used is 500kg / mu to 800kg / mu.

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