A multi-channel microfluidic chip and a detection method

Abstract

The application relates to a multi-channel micro-fluidic chip and a detection method, relates to the technical field of immune detection, and comprises a body and a syringe. At least one reaction channel is arranged in the body. The reaction channel is sequentially connected with a freeze-dried reagent reaction area and an antigen-antibody reaction area. A sample inlet and an air inlet hole are arranged on the upper surface of the body. An installation channel is arranged on one side of the body. The installation channel is communicated with the lower end of the sample inlet, the lower end of the air inlet hole and the reaction channel. The syringe is movably arranged in the installation channel. The outer wall of the syringe is sealingly connected with the inner wall of the installation channel. A through hole is arranged on the side wall of the syringe. The application has the effect that the syringe can accurately inject the detection sample into the reaction channel.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of immunoassay, and in particular to a multi-channel microfluidic chip and a detection method. BACKGROUND

[0002] Microfluidic chip technology is to integrate the basic operation units of sample preparation, reaction, separation and detection of biological, chemical and medical analysis processes on a micron-scale chip to automatically complete the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has developed into a new research field of biology, chemistry, medicine, fluid, electronics, materials, mechanics, etc.

[0003] In the related art, the microfluidic chip mainly includes a body, a plurality of reaction channels are arranged on the body, and each reaction channel has two reaction zones in sequence, and the two reaction zones have lyophilized reagents and antigens and antibodies in the two reaction zones respectively. When in use, a syringe is used to extract a detection sample from a test tube and then inject it into each reaction channel in sequence. The detection sample flows in the reaction channel and combines with the lyophilized reagents and antigens and antibodies in the reaction channel in sequence, and finally a LAMP or other type of detector is used to scan the combined substance to complete the detection.

[0004] For the above related technology, the applicant finds that the detection sample is mainly pushed into the reaction channel by the pushing force of the syringe. Because the inner diameter of the reaction channel is small, it is necessary to ensure that the liquid outlet of the syringe is accurately communicated with the inlet of the reaction channel, and it is also necessary to ensure that the connection between the liquid outlet of the syringe and the reaction channel is good in sealing, so as to ensure that the detection sample is injected into the reaction channel. However, the overall volume of the microfluidic chip is relatively small, which leads to a relatively large difficulty in the above-mentioned operation steps, and thus needs to be improved. SUMMARY

[0005] The purpose of the present application is to provide a multi-channel microfluidic chip and a detection method, which can reduce the operation difficulty of the microfluidic chip.

[0006] In a first aspect, the multi-channel microfluidic chip provided by the present application adopts the following technical solution:

[0007] The application discloses a multi-channel micro-fluidic chip, which comprises a body, at least one reaction channel in the body, a first piston and a second piston, a sample inlet and an air inlet hole arranged on the outer surface of the body respectively, an installation channel arranged in the body and extending in a preset direction, the sample inlet, the air inlet hole and the at least one reaction channel being spaced apart along the extension direction of the installation channel and being communicated with the installation channel respectively, the first piston being movably arranged in the installation channel along the extension direction of the installation channel, a containing cavity extending along the extension direction of the installation channel being arranged in the first piston, the second piston being movably arranged in the containing cavity, the first piston and the installation channel and the second piston and the first piston being airtightly connected respectively, and a through hole being arranged on the side wall of the first piston and being communicated with the containing cavity.

[0008] By adopting the technical scheme, when it is needed to accurately inject the detection sample into the corresponding detection channel, the syringe is first moved to make the through hole on the side wall of the syringe communicated with the sample inlet, so that the syringe can draw the detection sample in the sample inlet, then the syringe is moved again to make the through hole on the side wall of the syringe communicated with each reaction channel in turn, when the through hole on the side wall of the syringe is communicated with the reaction channel, the syringe is controlled to inject the detection sample into the reaction channel, only when the through hole on the side wall of the syringe is communicated with the reaction channel, the detection sample in the syringe can be accurately injected into the reaction channel, when the detection sample is injected into the reaction channel, the syringe is moved again to make the through hole on the side wall of the syringe aligned with the air inlet hole, so that the syringe can draw air, and finally the syringe is moved again to make the through hole on the side wall of the syringe communicated with each reaction channel in turn, when the through hole on the side wall of the syringe is communicated with the reaction channel, the syringe is controlled to inject air into the reaction channel, so as to push the detection sample to continuously move in the reaction channel by using the air, thereby making the detection sample enter the freeze-dried reagent reaction area and the antigen-antibody reaction area in turn to carry out corresponding combination.

[0009] Optionally, a waste liquid pool and a liquid return channel are arranged in the body respectively, the waste liquid pool is communicated with one end of each reaction channel away from the installation channel, the waste liquid pool is communicated with the installation channel through the liquid return channel, and a first air outlet hole is arranged on the outer surface of the body and communicated with the waste liquid pool.

[0010] By adopting the technical scheme, when the detection sample is used up, air or other medium can be used to continue to push the detection sample in the reaction channel to move, so that the detection sample enters the waste liquid pool, and the detection sample can be collected and destroyed after the detection is completed, and the air in the waste liquid pool can be directly discharged through the first air outlet hole after the detection sample enters the waste liquid pool, so that the situation that the detection sample is difficult to discharge into the waste liquid pool due to excessive air pressure in the waste liquid pool is avoided.

[0011] Optionally, the body is further provided with a cleaning liquid channel and an overflow pool respectively, two ends of the cleaning liquid channel are communicated with the mounting channel and the overflow pool respectively, the body is further provided with a flexible liquid storage cover communicated with the cleaning liquid channel, and a second exhaust hole communicated with the overflow pool is arranged on the outer surface of the body.

[0012] By adopting the above technical scheme, when the detection is completed, the flexible liquid storage cover can be punctured, so that the cleaning liquid in the flexible liquid storage cover flows into the cleaning liquid channel, and then the cleaning liquid is extracted by using the syringe to clean each reaction channel, thereby further avoiding the residual detection sample in the reaction channel, the overflow pool is used to temporarily store the cleaning liquid that is not actually needed for cleaning in the body, and the cleaning effect is ensured.

[0013] Optionally, the outer surface of the body is provided with a containing groove, the bottom of the containing groove is communicated with the cleaning liquid channel, and at least one protrusion is arranged in the bottom of the containing groove, and the flexible liquid storage cover is placed in the containing groove.

[0014] By adopting the above technical scheme, the containing groove can be used to position and store the flexible liquid storage cover, and when the cleaning liquid in the flexible liquid storage cover flows into the containing groove, the cleaning liquid can directly flow into the cleaning liquid channel, and the protrusion arranged in the containing groove can be pressed to conveniently and quickly puncture the flexible liquid storage cover.

[0015] Optionally, the syringe further comprises a hollow connecting sleeve, the mounting channel is arranged in the connecting sleeve, a plurality of spaced-apart communication channels are arranged in the side wall of the connecting sleeve, and the cleaning liquid channel, the waste liquid pool, the sample inlet and each reaction channel are sequentially communicated with the mounting channel through the single communication channel.

[0016] By adopting the above technical scheme, the thickness of the body is relatively thin, the connecting sleeve can ensure that the syringe has sufficient mounting space on the body, and the communication channels can realize the communication relationship between the mounting channel and the cleaning liquid channel, the lower end of the sample inlet and each reaction channel.

[0017] Optionally, the syringe comprises a first piston member and a second piston member, one end of the first piston member is open and the other end is closed, one end of the second piston member is inserted into the first piston member through the open end of the first piston member, and the opposite ends of the first piston member and the second piston member both extend out of the mounting channel, and the through hole is arranged on the side wall of the first piston member.

[0018] By adopting the technical scheme, the first piston member and the second piston member can constitute a syringe, and the ends of the first piston member and the second piston member oppositely arranged and extending out of the mounting channel can facilitate the detection personnel to move the syringe as a whole or drive the second piston member to move relative to the first piston member.

[0019] Optionally, the sample test tube is further provided, one end of the sample test tube is provided with a liquid outlet, one end of the sample test tube is inserted into the sample inlet, a one-way valve is arranged in the liquid outlet, a protrusion which can pass through the one-way valve is arranged at the bottom of the sample inlet, a through hole which is communicated with the mounting channel is arranged in the interior of the protrusion, and the sample inlet is communicated with the mounting channel through the through hole.

[0020] By adopting the technical scheme, the lower end of the sample test tube containing the detection sample is inserted into the sample inlet, and the protrusion at the bottom of the sample inlet passes through the one-way valve, so that the rapid supply of the detection sample can be realized, and the detection sample in the sample test tube does not need to be manually transferred into the sample inlet by using the syringe.

[0021] Optionally, the parts where the cleaning liquid channel, the sample inlet, the waste liquid pool and each reaction channel are communicated with the mounting channel are respectively provided with a valve, each valve comprises a rubber ring and a plurality of valve flaps, each valve flap is arranged on the inner wall of the rubber ring and is arranged circumferentially around the axis of the rubber ring, and each valve flap is used to isolate the two sides of the rubber ring.

[0022] By adopting the technical scheme, the valve can temporarily cut off the cleaning liquid channel, the sample inlet, the waste liquid pool and each reaction channel from the mounting channel, so that when the first piston member moves along a relatively long path and does not block the corresponding cleaning liquid channel, sample inlet, waste liquid pool or each reaction channel, the liquid in the cleaning liquid channel, sample inlet, waste liquid pool or reaction channel will not flow back.

[0023] In the second aspect, the application provides a detection method for the multi-channel microfluidic chip, which adopts the following technical scheme:

[0024] A detection method for a multi-channel microfluidic chip, comprising the following steps:

[0025] S1, moving the syringe to make the through hole communicated with the air inlet hole, controlling the syringe to suck a certain amount of air, then moving the syringe to make the through hole communicated with the sample inlet, and controlling the syringe to suck a sufficient amount of detection sample;

[0026] S2, moving the syringe to make the through hole communicated with each reaction channel in turn, and pushing a certain amount of detection sample into the corresponding reaction channel, after completion, moving the syringe to make the through hole directly communicated with the waste liquid pool, and pushing the remaining detection sample in the syringe into the waste liquid pool.

[0027] S3, moving the injector to make the through hole communicate with the air inlet hole, controlling the injector to suck enough air, then moving the injector to make the through hole communicate with each reaction channel in turn, injecting the air in the injector into each reaction channel, using the air to push the detection sample into the freeze-dried reagent reaction area to dissolve the freeze-dried reagent, and finally into the antigen-antibody reaction area to fully combine with the antigen-antibody;

[0028] S4, after the incubation time of the detection sample in each reaction channel ends, moving the injector to make the through hole communicate with the air inlet hole, controlling the injector to suck enough air, then moving the injector to make the through hole communicate with each reaction channel in turn, injecting the air in the injector into each reaction channel, using the air to send the detection sample in the reaction channel into the waste liquid pool;

[0029] S5, extruding the flexible liquid storage cover to make the cleaning liquid of the flexible liquid storage cover gradually fill the cleaning liquid channel, at the same time, moving the injector to make the through hole communicate with the air inlet hole, controlling the injector to suck enough air, then moving the injector to make the through hole communicate with the cleaning liquid channel, and controlling the injector to suck enough cleaning liquid;

[0030] S6, moving the injector to make the through hole communicate with each reaction channel in turn, controlling the injector to push a certain amount of cleaning liquid into the corresponding reaction channel to completely push the detection sample in the reaction channel into the waste liquid pool, and emptying the injector;

[0031] S7, moving the injector to make the through hole communicate with the air inlet hole, controlling the injector to suck enough air, then moving the injector to make the through hole communicate with each reaction channel in turn, injecting the air in the injector into each reaction channel, and using the air to push the residual cleaning liquid in the reaction channel into the waste liquid pool.

[0032] In summary, the present application includes at least one of the following beneficial technical effects:

[0033] 1. The injection of the detection sample only retains reciprocating control, which is easier to implement for the instrument, and the integrated design simplifies production and indirectly provides injection precision of the detection sample;

[0034] 2. The core of the first piston piece is the cavity of the second piston piece, which facilitates integrated injection molding production, simplifies the structure of the chip, and the injector is integrated on the chip, which are all disposable products, avoiding contamination of the instrument by the sample in the chip;

[0035] 3. The supply of the detection sample does not need the injector to be transferred to the chip, further reducing the possibility of contamination of the detection sample, effectively ensuring the detection precision of the detection sample, and being more convenient to use;

[0036] 4. The detection sample after detection can be timely concentrated on the chip, which is convenient for later discharge and destruction, and the detection activity is safer. BRIEF DESCRIPTION OF DRAWINGS

[0037] Figure 1 is a schematic diagram of the upper surface structure of the multi-channel microfluidic chip of embodiment 1 of the present application;

[0038] Figure 2 is a schematic diagram of the bottom surface structure of the multi-channel microfluidic chip of embodiment 1 of the present application;

[0039] Figure 3 is a schematic diagram of the perspective structure of the body of embodiment 1 of the present application;

[0040] Figure 4 is a schematic diagram of the structure of the syringe of embodiment 1 of the present application;

[0041] Figure 5 is a schematic diagram of the structure of the syringe of embodiment 1 of the present application; Figure 1

[0042] Figure 6 Figure 2

[0043] Figure 7 is a schematic diagram of the structure of the reaction channel of embodiment 1 of the present application;

[0044] Figure 8 is a schematic diagram of the setting of the first ring sleeve, the second ring sleeve and the third ring sleeve of embodiment 1 of the present application;

[0045] Figure 9 is a schematic diagram of the use of the sample test tube of embodiment 2 of the present application;

[0046] Figure 10 is a schematic diagram of the setting of the valve of embodiment 3 of the present application.

[0047] ​​​In the figure, 1, body; 11, sample inlet; 12, air inlet hole; 121, first ring; 13, cleaning liquid channel; 131, first channel; 132, second channel; 133, overflow pool; 134, second air outlet hole; 14, reaction channel; 141, freeze-dried reagent reaction area; 142, antigen-antibody reaction area; 15, connecting sleeve; 151, mounting channel; 152, mounting hole; 153, limiting hole; 154, communication channel; 16, containing groove; 161, placement area; 162, inner recess area; 163, protrusion; 164, through hole; 165, third ring; 17, waste liquid pool; 171, first air outlet hole; 172, second ring; 18, protrusion; 181, perforation; 19, valve; 191, rubber ring; 192, valve; 2, syringe; 21, first piston; 211, through hole; 22, second piston; 3, sample test tube; 31, liquid outlet; 32, one-way valve. DETAILED DESCRIPTION

[0048] The following will be described in detail with reference to the accompanying drawings. Figure 1 - the accompanying drawings Figure 10 The present application will be further described in detail.

[0049] Example 1:

[0050] A multi-channel microfluidic chip, referring to Figure 1 and Figure 2 , comprising a body 1 and a syringe 2, the syringe 2 is movably installed on one side of the body 1, and the body 1 is provided with a cleaning liquid channel 13, an air inlet hole 12, a sample inlet 11 and a plurality of reaction channels 14 (three are shown in the figure), a through hole 211 is provided on the side wall of the syringe 2 (not shown in the figure), by moving the syringe 2, the through hole 211 on the side wall of the syringe 2 can be communicated with the cleaning liquid channel 13, the air inlet hole 12, the sample inlet 11 or the reaction channel 14; wherein the body 1 comprises a main body and a cover plate (not shown in the figure), the cleaning liquid channel 13 and each reaction channel 14 are arranged on the bottom surface of the main body, and the cover plate is arranged on the bottom surface of the main body.

[0051] Referring to Figure 2 and Figure 3A connecting sleeve 15 is arranged on one side of the body 1, the cross section of the connecting sleeve 15 is circular, and the radius of the connecting sleeve 15 is greater than the thickness of the body 1. An installation channel 151 coaxial with the connecting sleeve 15 is arranged in the connecting sleeve 15, and the installation channel 151 is obtained by expanding one side of the body 1. In the illustrated embodiment, the installation channel 151 is a circular channel, and the radius of the installation channel 151 is greater than the thickness of the body 1. In other embodiments, the installation channel 151 can also be circular, elliptical or other shapes. The installation channel 151 includes an installation hole 152 and a limiting hole 153 arranged coaxially and in communication with each other. The inner diameter of the installation hole 152 is greater than the inner diameter of the limiting hole 153. A plurality of communication channels 154 arranged along the axis of the connecting sleeve 15 are arranged in the side wall of the connecting sleeve 15. The upper end of each communication channel 154 communicates with the installation hole 152, and the lower end of each communication channel 154 communicates with the bottom surface of the body. The cleaning liquid channel, the lower end of the sample inlet 11 and each reaction channel 14 communicate with each other through the communication channels 154 to communicate with the installation hole 152.

[0052] Referring to Figure 3 and Figure 4 , the syringe 2 includes a first piston member 21 and a second piston member 22. One end of the first piston member 21 is open and the other end is closed. One end of the second piston member 22 is inserted into the first piston member 21 through the open end of the first piston member 21. A through hole 211 is arranged on the side wall of the end of the first piston member 21 away from the second piston member 22. When the syringe 2 is installed on the body 1, the open end of the first piston member 21 is inserted into the installation hole 152, and the other end of the first piston member 21 passes through the limiting hole 153 and extends out of the connecting sleeve 15. The end of the second piston member 22 extending out of the first piston member 21 passes through the installation hole 152.

[0053] Referring to Figure 5 and Figure 6 , the upper surface of the body 1 is provided with a containing groove 16. The containing groove 16 includes a placement area 161 and an inner recess area 162. The upper side of the placement area 161 communicates with the upper surface of the body 1. The inner recess area 162 is arranged in a recessed manner on the bottom of the placement area 161. A through hole 164 communicating with the cleaning liquid channel 13 is arranged at the lowest part of the inner recess area 162. At least one convex spike 163 is arranged in the inner recess area 162 in a circumferential equidistant manner around the axis of the through hole 164. A flexible liquid storage cover made of plastic film (not shown in the figure) is placed in the containing groove 16. The flexible liquid storage cover is filled with cleaning liquid. When the flexible liquid storage cover is placed in the containing groove 16, the bottom edge of the flexible liquid storage cover is in contact with the bottom surface of the placement area 161, and there is a gap between the bottom of the thin capsule and the convex spike 163.

[0054] Referring to Figure 5 and Figure 6The body 1 is further provided with an overflow pool 133 and a second exhaust hole 134, the overflow pool 133 is arranged in the lower side of the body 1 and located on the lower surface of the body, and the second exhaust hole 134 communicates with the two side surfaces of the body; wherein the cleaning liquid channel 13 comprises a first channel 131 and a second channel 132, two ends of the first channel 131 are communicated with the through hole 164 and the corresponding communication channel 154 respectively, two ends of the second channel 132 are communicated with the overflow pool 133 and the middle part of the first channel 131 respectively, and the end of the overflow pool 133 away from the second channel 132 is communicated with the lower end of the second exhaust hole 134.

[0055] With reference to Figure 7 The bottom surface of the body is further provided with at least one reaction area arranged along the length direction of the body, each reaction area comprises a freeze-dried reagent reaction area 141 and an antigen-antibody reaction area 142 arranged along the width direction of the body, each reaction channel 14 is matched with each reaction area respectively, and each reaction channel 14 is sequentially arranged through the freeze-dried reagent reaction area 141 and the antigen-antibody reaction area 142 at the corresponding position; wherein the part of each reaction channel 14 between the freeze-dried reagent reaction area 141 and the antigen-antibody reaction area 142 is arranged in a continuous “S” shape.

[0056] With reference to Figure 7 The bottom surface of the body is further provided with a waste liquid pool 17, the waste liquid pool 17 is located on the side of the body away from the connecting sleeve 15, the end of each reaction channel 14 away from the mounting channel 151 is communicated with the waste liquid pool 17, the waste liquid pool 17 is further directly communicated with one of the communication channels 154, and the body 1 is further provided with a first exhaust hole 171 communicated with the upper surface of the body 1 and the waste liquid pool 17 respectively.

[0057] With reference to Figure 8 The upper surface of the body 1 is further provided with a first ring sleeve 121, a second ring sleeve 172 and a third ring sleeve 165, the first ring sleeve 121 is coaxially arranged with the sample inlet, the second ring sleeve 172 is arranged in communication with the first exhaust hole 171, and the third ring sleeve 165 is arranged in communication with the through hole 164.

[0058] The implementation principle of the embodiment of the application is:

[0059] In use, first, the sample is placed into the sample inlet 11, at this time, the syringe 2 is moved so that the syringe 2 can extract the detection sample, and then the syringe 2 extracting the detection sample is moved to the position so that the syringe 2 can inject the detection sample into the specified reaction channel 14, so as to realize the rapid and accurate injection of the detection sample.

[0060] After the injection of the sample into the reaction channel 14, the cooperation between the injector 2 and the air inlet 12 is used again to enable the extraction of air from the inside of the injector 2, and then the injector 2 is moved again to enable the injection of air into each reaction channel 14 in sequence, so as to achieve the effect of using air to push the detection sample to pass through the freeze-dried reagent reaction area 141 and the antigen-antibody reaction area 142 in sequence, thereby realizing the combination of the detection sample with the freeze-dried reagent and the antigen-antibody.

[0061] After the combination of the detection sample is completed, the injector 2 is used to extract the cleaning liquid, and then the injector 2 is moved again to enable the injection of the cleaning liquid into each reaction channel 14 in sequence, so as to realize the cleaning of the reaction channel 14 and discharge the residual detection sample in the reaction channel 14 into the waste liquid pool 17, thereby facilitating the centralized collection and destruction of the detection sample.

[0062] Embodiment 2:

[0063] With reference to Figure 9 The difference between the embodiment of the present application and embodiment 1 is that the embodiment of the present application further comprises a sample test tube 3, the upper end of the sample test tube 3 is open, a liquid outlet 31 is arranged at one end of the sample test tube 3, a one-way valve 32 for preventing the detection sample in the sample test tube 3 from flowing out is arranged in the liquid outlet 31, and the one end of the sample test tube 3 is installed in the sample inlet 11 in a plug-in manner; wherein a protrusion 18 that can pass through the one-way valve 32 is arranged at the bottom of the sample inlet 11, the protrusion 18 is arranged on the surface of the cover, and a through hole 181 is arranged in the inside of the protrusion 18, the lower end of the through hole 181 communicates with the side wall of the protrusion 18, and when the cover is installed on the main body, the lower end of the through hole 181 communicates with the corresponding communication channel 154.

[0064] When it is necessary to supply the detection sample to the multi-channel microfluidic chip, it is only necessary to insert the lower end of the sample test tube 3 containing the detection sample into the sample inlet 11 and make the protrusion 18 at the bottom of the sample inlet 11 pass through the one-way valve 32, at this time, the detection sample in the sample test tube 3 can be discharged through the through hole 181 in the inside of the protrusion 18.

[0065] Embodiment 3:

[0066] With reference to Figure 10 The difference between the embodiment of the present application and embodiment 1 is that in the embodiment of the present application, a valve 19 is arranged in the part where each communication channel 154 communicates with the installation channel 151, each valve 19 comprises a rubber ring 191 and a valve 192, the valve 192 is arranged in a circumferential direction around the axis of the rubber ring 191, and when each valve 192 is not subjected to external force, each valve 192 is in close contact to achieve the effect of isolating the two sides of the rubber ring 191.

[0067] When the syringe 2 draws or discharges the liquid, each valve 192 will be bent due to the negative pressure, thereby enabling the liquid in the chip body 1 to flow through the rubber ring 191.

[0068] The application further discloses a detection method for the multi-channel microfluidic chip, which comprises the following steps: S1, moving the syringe 2 to make the through hole 211 communicate with the air inlet hole 12, controlling the syringe 2 to suck a certain amount of air, then moving the syringe 2 to make the through hole 211 communicate with the sample inlet, and controlling the syringe 2 to suck a sufficient amount of detection sample;

[0069] S2, moving the syringe 2 to make the through hole 211 communicate with each reaction channel 14 in sequence, and pushing a certain amount of detection sample into the corresponding reaction channel, after completion, moving the syringe 2 to make the through hole 211 directly communicate with the waste liquid pool 17, and pushing the remaining detection sample in the syringe 2 into the waste liquid pool 17;

[0070] S3, moving the syringe 2 to make the through hole 211 communicate with the air inlet hole 12, controlling the syringe 2 to suck a sufficient amount of air, then moving the syringe 2 to make the through hole 211 communicate with each reaction channel 14 in sequence, and injecting the air in the syringe 2 into each reaction channel 14, so as to push the detection sample into the freeze-dried reagent reaction area 141 to dissolve the freeze-dried reagent, and finally into the antigen-antibody reaction area 142 to fully combine with the antigen-antibody;

[0071] S4, after the incubation time of the detection sample in each reaction channel 14 ends, moving the syringe 2 to make the through hole 211 communicate with the air inlet hole 12, controlling the syringe 2 to suck a sufficient amount of air, then moving the syringe 2 to make the through hole 211 communicate with each reaction channel 14 in sequence, and injecting the air in the syringe 2 into each reaction channel 14, so as to push the detection sample in the reaction channel 14 into the waste liquid pool 17;

[0072] S5, extruding the flexible liquid storage cover to make the cleaning liquid in the flexible liquid storage cover gradually fill the cleaning liquid channel, and moving the syringe 2 to make the through hole 211 communicate with the air inlet hole 12, controlling the syringe 2 to suck a sufficient amount of air, then moving the syringe 2 to make the through hole 211 communicate with the cleaning liquid channel, and controlling the syringe 2 to suck a sufficient amount of cleaning liquid;

[0073] S6, moving the syringe 2 to make the through hole 211 communicate with each reaction channel 14 in sequence, and controlling the syringe 2 to push a certain amount of cleaning liquid into the corresponding reaction channel 14, so as to completely push the detection sample in the reaction channel 14 into the waste liquid pool 17, and empty the syringe 2;

[0074] S7, the mobile injector 2 is moved to make the through hole 211 communicate with the air inlet hole 12, the injector 2 is controlled to suck a sufficient amount of air, then the mobile injector 2 is moved to make the through hole 211 communicate with each reaction channel 14 in turn, and the air in the injector 2 is injected into each reaction channel 14, so that the residual cleaning liquid in the reaction channel 14 is pushed into the waste liquid pool 17 by the air.

[0075] The embodiments of the present specific embodiment are the preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, wherein the same parts are denoted by the same reference numerals. Therefore, any equivalent changes made according to the structure, shape and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A multi-channel microfluidic chip comprising a body (1) having at least one reaction channel (14) therein, characterized in that, The application also comprises a first piston (21) and a second piston (22), the body (1) is provided with a sample inlet (11) and an air inlet (12) on the outer surface respectively, the body (1) is provided with an installation channel (151) extending along a preset direction, the sample inlet (11), the air inlet (12) and at least one reaction channel (14) are spaced apart along the extension direction of the installation channel (151) and communicated with the installation channel (151) respectively, the first piston (21) is movably arranged in the installation channel (151) along the extension direction of the installation channel (151), the first piston (21) is provided with a containing cavity extending along the extension direction of the installation channel (151), the second piston (22) is movably arranged in the containing cavity, the first piston (21) and the installation channel (151) and the second piston (22) and the first piston (21) are airtightly connected respectively, the side wall of the first piston (21) is provided with a through hole (211) communicating with the containing cavity, the body (1) is provided with a waste liquid pool (17) and a liquid return channel respectively, the waste liquid pool (17) is communicated with one end of each reaction channel (14) away from the installation channel (151), the waste liquid pool (17) is communicated with the installation channel (151) through the liquid return channel, the outer surface of the body (1) is provided with a first exhaust hole (171) communicated with the waste liquid pool (17), the body (1) is further provided with a cleaning liquid channel (13) and an overflow pool (133) respectively, two ends of the cleaning liquid channel (13) are communicated with the installation channel (151) and the overflow pool (133) respectively, the body (1) is further provided with a flexible liquid storage cover communicated with the cleaning liquid channel (13), the outer surface of the body (1) is provided with a second exhaust hole (134) communicated with the overflow pool (133), the application further comprises a sample test tube (3), one end of the sample test tube (3) is provided with a liquid outlet (31), one end of the sample test tube (3) is inserted into the sample inlet (11), the liquid outlet (31) is provided with a one-way valve (32), the bottom of the sample inlet (11) is provided with a protrusion (18) capable of penetrating the one-way valve (32), the inside of the protrusion (18) is provided with a perforation (181) communicated with the installation channel (151), the sample inlet (11) is communicated with the installation channel (151) through the perforation (181).

2. The multi-channel microfluidic chip according to claim 1, wherein, The outer surface of the body (1) is provided with a containing groove (16), the bottom of the containing groove (16) is communicated with the cleaning liquid channel (13), the bottom of the containing groove (16) is further provided with at least one protrusion (163), the flexible liquid storage cover is placed in the containing groove (16).

3. The multi-channel microfluidic chip of claim 1, wherein, Further comprising a hollow connecting sleeve (15), the installation channel (151) is arranged in the connecting sleeve (15), and a plurality of spaced communication channels (154) are arranged in the side wall of the connecting sleeve (15), the cleaning liquid channel (13), the waste liquid pool (17), the sample inlet (11) and each reaction channel (14) are communicated with the installation channel (151) through the single communication channel (154) in sequence.

4. The multi-channel microfluidic chip of claim 1, wherein, The part where the cleaning liquid channel (13), the sample inlet (11), the waste liquid pool (17) and each reaction channel (14) are communicated with the installation channel (151) is provided with a valve (19), and each valve (19) comprises a rubber ring (191) and a plurality of valve plates (192), each valve plate (192) is arranged on the inner wall of the rubber ring (191) and is arranged circumferentially around the axis of the rubber ring (191), and each valve plate (192) is used to isolate the two sides of the rubber ring (191).

5. A detection method for the multichannel microfluidic chip according to any one of claims 1-4, characterized in that, The method comprises the following steps: S1, moving the injector (2) to make the through hole (211) communicate with the air inlet hole (12), controlling the injector (2) to suck a certain amount of air, then moving the injector (2) to make the through hole (211) communicate with the sample inlet (11), and controlling the injector (2) to suck a sufficient amount of detection sample; S2, moving the injector (2) to make the through hole (211) communicate with each reaction channel (14) in sequence, and pushing a certain amount of detection sample into the corresponding reaction channel (14), after completion, moving the injector (2) to make the through hole (211) directly communicate with the waste liquid pool (17), and pushing the remaining detection sample in the injector (2) into the waste liquid pool (17); S3, moving the injector (2) to make the through hole (211) communicate with the air inlet hole (12), controlling the injector (2) to suck a sufficient amount of air, then moving the injector (2) to make the through hole (211) communicate with each reaction channel (14) in sequence, and injecting the air in the injector (2) into each reaction channel (14), using the air to push the detection sample into the freeze-dried reagent reaction area (141) to dissolve the freeze-dried reagent, and finally into the antigen-antibody reaction area (142) to fully combine with the antigen-antibody; S4, after the incubation time of the detection sample in each reaction channel (14) ends, moving the injector (2) to make the through hole (211) communicate with the air inlet hole (12), controlling the injector (2) to suck a sufficient amount of air, then moving the injector (2) to make the through hole (211) communicate with each reaction channel (14) in sequence, and injecting the air in the injector (2) into each reaction channel (14), using the air to send the detection sample in the reaction channel (14) into the waste liquid pool (17); S5, extruding the flexible liquid storage cover to gradually fill the cleaning liquid channel with the cleaning liquid of the flexible liquid storage cover, at the same time, moving the injector (2) to make the through hole (211) communicate with the air inlet hole (12), controlling the injector (2) to suck a sufficient amount of air, then moving the injector (2) to make the through hole (211) communicate with the cleaning liquid channel (13), and controlling the injector (2) to suck a sufficient amount of cleaning liquid; S6, moving the injector (2) so that the through hole (211) is communicated with each reaction channel (14) in turn, and controlling the injector (2) to push a certain amount of washing liquid into the corresponding reaction channel (14) so as to push the detection sample in the reaction channel (14) into the waste liquid pool (17) completely and empty the injector (2); S7, moving the injector (2) so that the through hole (211) is communicated with the air inlet hole (12), and controlling the injector (2) to suck a sufficient amount of air, then moving the injector (2) so that the through hole (211) is communicated with each reaction channel (14) in turn, and injecting the air in the injector (2) into each reaction channel (14) so as to push the residual washing liquid in the reaction channel (14) into the waste liquid pool (17) by the air.

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