A plant-based moisture-absorbing disease-preventing isolation agent for silkworms and a preparation method thereof

Abstract

The present invention proposes a plant-based hygroscopic disease prevention isolator for silkworms and a preparation method thereof, and belongs to the technical field of silkworm breeding and disease prevention. In view of the fact that the existing technology mainly uses chemical disinfectants such as lime, chlorine-containing preparations, and aldehyde preparations to disinfect silkworms, which has the problem of harm to the human body and environmental pollution, the present invention uses medicinal plants Eupatorium adenophorum and Bidens pilosa as substrates, adds mulberry branches, plant ash and a small amount of lime in proportion to prepare a green and environmentally friendly hygroscopic disease prevention isolator for silkworms that can reasonably simplify the silkworm breeding operation process, reduce the harm caused by disinfection and disease prevention preparations to the human body, and reduce the damage to the ecological environment. The isolator can be used at all ages of silkworm breeding, and the sand removal operation can be removed when used in the young silkworm stage, and the sand removal operation can be reduced when used in the adult silkworm stage, which can effectively reduce the occurrence of silkworm diseases, and at the same time can ensure a high cocooning rate and cocoon layer rate, and has considerable social benefits and promotion value.

Description

Technical Field

[0001] The invention belongs to the technical field of silkworm breeding and disease prevention, and particularly relates to a plant-based moisture-absorbing disease-preventing isolation agent for silkworms and a preparation method thereof. Background Art

[0002] In the silkworm feeding process, the temperature of the silkworm room and the humidity of the silkworm bed are generally required to be 26℃-28℃, 75%RH. In remote mountainous areas where silkworm industry is concentrated, the natural temperature and humidity conditions are difficult to meet the feeding requirements. Such an environment is prone to viral and bacterial diseases of silkworms, resulting in a low cocooning rate, causing significant losses to the economic income of silkworm farmers, greatly reducing the willingness of silkworm farmers to raise silkworms, and causing serious obstacles to the development of my country's silkworm industry. Therefore, the comprehensive prevention and control of silkworm diseases has always been an important part of rural silkworm breeding. The silkworm room, silkworm tools, silkworm beds and mulberry leaves are disinfected, and strict disinfection is carried out at multiple times before, during and after silkworm breeding throughout the entire breeding process.

[0003] Traditional commonly used disinfectants are mainly chemical, including lime, chlorine-containing preparations, aldehyde preparations, etc. These disinfectants are usually prepared into solutions and sprayed in the silkworm room and its surroundings, or soaked in silkworm tools, etc. They can also be sprinkled as powders on silkworm seats and silkworm bodies. They have effective killing capabilities against major pathogens of silkworms, including microsporidia, nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus, Beauveria bassiana, bacteria, etc.

[0004] Lime is the earliest disinfectant used in silkworm breeding, and it is known as "a handful of lime for all diseases" in the prevention and treatment of silkworm diseases. Fresh lime not only has an effective killing effect on all silkworm pathogens, but can also be used as a powder, water-soluble solvent and compound auxiliary agent. It can be used for disinfection of all disinfection objects (silkworm bodies, silkworm seats, silkworm rooms and silkworm tools, etc.) and all silkworm rearing time periods. In particular, it has a moisture absorption function for the disinfection of silkworm seats and silkworm bodies, controls the rearing microenvironment, and can effectively inhibit the spread of pathogens and prevent the spread of diseases. The most common chlorine-containing preparation is bleaching powder, which is currently the most widely used disinfectant. Fresh bleaching powder solution has strong alkalinity and has strong killing effect on all silkworm pathogens. Different disinfection objects have different concentration requirements, that is, different effective chlorine content requirements. When used, the effective chlorine content needs to be measured by iodine titration. Bleaching powder and fresh lime are mixed in a ratio of 1:12 to form anti-rigidity powder, which is used for disinfection of silkworm seats and silkworm bodies in the young silkworm period, and has a good preventive effect on rigidity. The effective disinfection effect of bleaching powder and its aqueous solution is greatly reduced after being placed for a period of time; aldehyde preparations mainly refer to formaldehyde disinfectants, which are often prepared into formalin aqueous solutions containing 2% formaldehyde, and are used to disinfect silkworm room silkworm tools before and after silkworm rearing. They can also be used in combination with fresh lime powder or block lime to make water solvents or fumigants to spray, soak or fumigate silkworm room silkworm tools. The use of formalin and its compound agents requires the silkworm room to be sealed for at least 24 hours, and the silkworm room silkworm tools can be used after the smell of the medicine has dissipated for a week. Moreover, formaldehyde is a well-known carcinogen that is most harmful to the human body.

[0005] For example, a Chinese patent with publication number CN102939976A and titled "A Disinfection and Isolation Agent for Silkworm Body and Silkworm Seats for Feeding Silkworms" discloses that 25% carbendazim powder and talcum powder are prepared in a weight ratio of 1:5-10 for silkworms in the 1-3 instar and 4-5 instar stages to disinfect silkworm bodies and silkworm seats; another Chinese patent with publication number CN106234322A and titled "A Method for Preventing Diseases in Silkworm Breeding" discloses that 25% carbendazim powder and talcum powder are prepared in a weight ratio of 1:5-10 for silkworms in the 1-3 instar and 4-5 instar stages to disinfect silkworm bodies and silkworm seats; The Chinese patent proposes to use a mixture of 2%-3% formalin and 1%-2% lime to disinfect the silkworm room, silkworm tools and silkworm breeding environment before silkworms are laid, a mixture of formalin and lime to disinfect the environment inside the silkworms, bleaching powder to prevent silkworm stiffness and silkworm disease spirit to disinfect silkworm bodies and silkworm seats, and 2% formalin solution to spray the mulberry leaves for disinfection before mulberry planting. This prior art uses a disinfectant made of formalin and lime to disinfect the entire silkworm breeding process.

[0006] However, the chemical disinfectants in the prior art are subject to many factors when used, which affect their bactericidal effect, such as requiring pathogens to be fully exposed, using different disinfection methods and agents for different pathogens and disinfection objects, and the preparation, concentration and action time of the disinfectants and the temperature during disinfection, etc., all need to be considered and reasonably arranged one by one. Although chemical disinfectants have a preventive effect on silkworm diseases, a large amount of powder is produced during the preparation process, which is easily inhaled into the human body (such as lime), or emits a pungent odor (such as formalin) after being dissolved in water, causing serious harm to the human body, or is insoluble in water and only soluble in other organic chemical solvents (such as carbendazim). Moreover, after the breeding garbage containing such disinfectants is discarded, it causes serious pollution to the soil and air, which will greatly harm the natural environment on which we depend for survival. Summary of the invention

[0007] In view of the problems existing in the above-mentioned prior art, the present invention proposes a plant-based hygroscopic disease prevention isolator for silkworms and a preparation method thereof. Starting from the actual situation of silkworm breeding, the common medicinal plants Eupatorium adenophorum and Bidens pilosa that are suitable for growth in the local area are used as the main components of the isolator. Combined with the comprehensive utilization of raw materials in the sericulture industry, mulberry branches are added as secondary components, and then compounded with common plant ash and a very small amount of lime in rural areas in proportion to form the main ingredient of the isolator. A composite formula of a hygroscopic disease prevention isolator based on medicinal plant sources is creatively prepared to achieve the purpose of reasonably simplifying the silkworm breeding operation process, reducing the harm caused by disinfection and disease prevention preparations to the human body, and reducing ecological environmental damage. The present invention obtains an effective hygroscopic, isolating and disease prevention agent formula through experimental matching. The isolator can replace the currently used chemical disinfectants such as bleaching powder and quicklime to reduce ecological damage. At the same time, the use of the isolator can reduce the sand removal operation at all ages of silkworm breeding and ensure the healthy growth of silkworms. At present, there is no application of the same category of isolating agents as the present invention in the field of silkworm breeding and disease prevention, which has great application market and promotion value.

[0008] The above technical objectives of the present invention are achieved through the following technical solutions:

[0009] A plant-based moisture-absorbing disease-preventing isolation agent for silkworms comprises a main ingredient and an auxiliary ingredient, wherein the main ingredient comprises eupatorium adenophorum, bidens pilosa, mulberry branch, plant ash and lime powder, and the components by volume are: 10-80 parts of eupatorium adenophorum, 10-80 parts of bidens pilosa, 5-10 parts of mulberry branch, 1-5 parts of plant ash and 1-5 parts of lime powder, and the mixing ratio of the main ingredient to the auxiliary ingredient is 1-5:1-10.

[0010] To achieve the above purpose, further, the main ingredients are composed of 30-60 parts of Eupatorium adenophorum, 30-60 parts of Bidens pilosa, 3-8 parts of mulberry branches, 2-5 parts of wood ash, and 1-3 parts of lime powder in parts by volume.

[0011] Furthermore, the main ingredients are composed of 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of wood ash, and 2 parts of lime powder in parts by volume.

[0012] By adopting the above technical scheme, further, the dosage form of the main ingredient composition is one of granules, powders, tablets, hard capsules, soft capsules, mixtures, pills, pellets, and microcapsules.

[0013] Furthermore, the auxiliary material component is sand or soil commonly found in the area where silkworms are raised.

[0014] The present invention also provides a method for preparing a plant-based moisture-absorbing disease-preventing isolation agent for silkworms, which comprises at least the following steps:

[0015] Step 1: selecting Eupatorium adenophorum, Bidens pilosa, mulberry branch, plant ash, and lime powder as main ingredients, and airing, cutting, and drying Eupatorium adenophorum, Bidens pilosa, and mulberry branch;

[0016] Step 2: Grind the dried, cut Eupatorium adenophorum, Bidens pilosa, and Morus alba into granules or powder respectively;

[0017] Step 3: sieve the ground Eupatorium adenophorum, Bidens pilosa, and Morus alba respectively, using a sieve of 50 to 300 meshes to select the desired granules or powders;

[0018] Step 4: Spread out the selected granules or powders of Eupatorium adenophorum, Bidens pilosa, and Morus alba respectively and air them;

[0019] Step 5: mixing the granules or powders of Eupatorium adenophorum, Bidens pilosa, and Morus alba in step 4 with plant ash and lime powder to prepare a dosage form of the main ingredient composition;

[0020] Step 6: Mix the dosage form of the main ingredient composition in step 5 with the auxiliary ingredients in a ratio of 1-5:1-10 to prepare a moisture-absorbing disease-preventing isolation agent for silkworms.

[0021] Through the above technical scheme, further, the drying in step 1 is performed by exposing to the midday sun for 3 hours, and the dried stems of Eupatorium, Bidens pilosa, and mulberry branches are turned over every hour, the cut length is 5 cm, and the oven is set at 65°C for 24 hours of drying.

[0022] Through the above technical solution, further, the drying in step 4 is performed by exposing to the midday sun for 2 hours, or using an oven set at 65° C. to dry for 30 minutes.

[0023] Through the above technical scheme, further, the mixing proportions of the main ingredients in step 5 are as follows by volume: 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of wood ash, and 2 parts of lime powder.

[0024] The present invention has the following beneficial effects by adopting the above technical solution:

[0025] 1. The isolating agent prepared by the present invention is a substrate with common medicinal plants Eupatorium adenophorum and Bidens pilosa as main ingredients. These two substrates are commonly found in open spaces and hillsides around silkworm rearing, making it convenient to obtain materials locally. The medicinal value of Eupatorium adenophorum and Bidens pilosa is utilized in silkworm disease prevention, which can not only reduce the occurrence of silkworm diseases, but also the amount of lime added to the isolating agent is only 1 / 25 of the traditional amount of lime used, greatly reducing the amount of lime used, being green and environmentally friendly, and effectively reducing the harmful effects on the ecological environment.

[0026] 2. The isolating agent of the present invention has the effects of moisture absorption and disease prevention. When used, it is evenly sprinkled in the silkworm seat, which has a moisture absorption effect on silkworm excrement (residual mulberry leaves and silkworm excrement) to ensure the dry growth environment of silkworms; it is mainly sprinkled on the position of diseased silkworms to prevent the spread of pathogens in the silkworm seat and greatly reduce the incidence of diseases. The isolating agent can be used at all ages of silkworm rearing. When used in the young silkworm stage, the sand removal operation can be eliminated, and when used in the adult silkworm stage, the sand removal operation can be reduced. It has considerable social benefits and promotion prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments.

[0028] Figure 1 This is a comparison chart of the weight of a single silkworm of each experimental group of the present invention on the third day of the fifth instar;

[0029] Figure 2 It is a graph showing the dryness of the silkworm seats of the silkworms of each experimental group of the present invention on the third day of the fifth instar;

[0030] Figure 3 It is a comparison chart of the cocoon-making rates of silkworms in various experimental groups of the present invention;

[0031] Figure 4 is a comparison chart of the cocoon layer rates of silkworms in various experimental groups of the present invention;

[0032] Figure 5 This is a comparison chart of the amount of lime used for all ages and the amount of lime used for the compound agent of the present invention;

[0033] Figure 6 This is a growth chart of silkworms at the 2nd instar without adding any materials during the experimental process of the present invention;

[0034] Figure 7 A growth chart of Eupatorium adenophorum was added to the silkworms at the 2nd instar during the experimental process of the present invention;

[0035] Figure 8 The growth diagram of the silkworms at the 2nd instar with the addition of mugwort leaves during the experimental process of the present invention;

[0036] Fig. 9 A growth chart of Eupatorium adenophorum was added to the 4th instar silkworms during the experimental process of the present invention;

[0037] Fig.10 The growth diagram of the silkworms at the 4th instar with the addition of mugwort leaves during the experimental process of the present invention;

[0038] Fig.11 This is a growth chart of lime added to silkworms at the 4th instar during the experimental process of the present invention. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0040] refer to Figure 1-11 The present invention provides a plant-based hygroscopic disease-preventing isolation agent for silkworms and a preparation method thereof, comprising a main ingredient and an auxiliary ingredient, wherein the mixing ratio of the main ingredient to the auxiliary ingredient is 1-5:1-10, wherein the main ingredient comprises Eupatorium adenophorum, Bidens pilosa, mulberry branch, plant ash, and lime powder, and the components by volume are 10-80 parts of Eupatorium adenophorum, 10-80 parts of Bidens pilosa, 5-10 parts of mulberry branch, 1-5 parts of plant ash, and 1-5 parts of lime powder, and the auxiliary ingredient is sand or soil commonly found in places where silkworms are raised.

[0041] Preferably: the main ingredients are composed of 30-60 parts of Eupatorium adenophorum, 30-60 parts of Bidens pilosa, 3-8 parts of mulberry branches, 2-5 parts of wood ash, and 1-3 parts of lime powder; more preferably: 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of wood ash, and 2 parts of lime powder.

[0042] By selecting the above main ingredients and auxiliary ingredients, the silkworm moisture absorption and disease prevention isolation agent of the present invention can be further prepared according to the following method, including the following steps:

[0043] Step 1: selecting Eupatorium adenophorum, Bidens pilosa, mulberry branch, plant ash, and lime powder as main ingredients, exposing Eupatorium adenophorum, Bidens pilosa, and mulberry branch to the sun at noon for 3 hours, turning them over every 1 hour during the drying process, cutting them into small sections with a length of 5 cm after the drying, and then drying them in an oven set at 65° C. for 24 hours;

[0044] Step 2: Grind the dried, cut Eupatorium adenophorum, Bidens pilosa, and Morus alba into granules or powder respectively;

[0045] Step 3: sieve the ground Eupatorium adenophorum, Bidens pilosa, and Morus alba respectively, using a sieve of 50 to 300 meshes to select the desired granules or powders;

[0046] Step 4: Spread out the selected granules or powders of Eupatorium adenophorum, Bidens pilosa, and Morus alba under the midday sun for 2 hours or use an oven set at 65° C. to dry for 30 minutes;

[0047] Step 5: Mix the granules or powders of Eupatorium adenophorum, Bidens pilosa, and mulberry branches in step 4 with ash and lime powder in the form of 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of ash, and 2 parts of lime powder by volume to prepare a dosage form of the main ingredient composition;

[0048] Step 6: Mix the dosage form of the main ingredient composition in step 5 with the auxiliary ingredients in a ratio of 1-5:1-10 to prepare a moisture-absorbing disease-preventing isolation agent for silkworms.

[0049] The isolating agent of the invention can replace the commonly used lime and be used on the silkworm breeding bed. It is green and environmentally friendly and has the functions of moisture absorption and disease prevention. It has the functions of moisture absorption and disease prevention in all ages of silkworm breeding.

[0050] Embodiment 1:

[0051] Use for silkworms of all ages: The young silkworm stage (1st to 3rd instar) is an important period for the healthy growth of silkworms. At this time, the silkworm beds are mainly dried to prevent diseases. During the 1st and 2nd instars, the isolating agent only uses the main ingredient, which is applied once before sleeping and once when waking up; during the 3rd instar, the main ingredient and the auxiliary ingredient in the isolating agent are mixed in a ratio of 2:1, and applied once during the instar, before sleeping, and once when waking up; during the adult silkworm stage (4th to 5th instar), silkworms grow rapidly, and isolation is the main way to prevent diseases. During the 4th instar, the main ingredient and the auxiliary ingredient in the isolating agent are mixed in a ratio of 1:2 or 1:3, and applied twice during the instar, before sleeping, and once when waking up; during the 5th instar, the main ingredient and the auxiliary ingredient in the isolating agent are mixed in a ratio of 1:5, and applied once a day.

[0052] Embodiment 2:

[0053] Use of co-rearing of young silkworms: Co-rearing of young silkworms is the main silkworm breeding technology currently implemented. It is concentrated in one place to raise silkworms in large quantities, and has high requirements for silkworm breeding technology and environment. In the first instar, the isolating agent only uses the main ingredient, and is applied once in the middle of the instar and before sleep; in the second instar, the main ingredient and the auxiliary ingredient in the isolating agent are mixed in a ratio of 1:1 or 1:2, and applied once when waking up, in the middle of the instar, and before sleep; in the third instar, the main ingredient and the auxiliary ingredient in the isolating agent are mixed in a ratio of 2:1, and applied once when waking up, twice in the middle of the instar, and once before sleep.

[0054] Embodiment 3:

[0055] Use when silkworm disease occurs: When the disease occurs in the young silkworm stage, sprinkle the isolation agent on the entire silkworm seat. The isolation agent only uses the main ingredient, and it is used before each mulberry feeding; when the disease occurs in the adult silkworm stage, use the isolation agent promptly and focus on the location where the silkworm disease occurs. The main ingredient and auxiliary ingredient of the isolation agent are mixed in a ratio of 1:1. At the same time, use the isolation agent on the entire silkworm seat. The main ingredient and auxiliary ingredient of the isolation agent are mixed in a ratio of 1:3, and it is used before each mulberry leaf feeding.

[0056] In order to further demonstrate the hygroscopic and disease-preventing effects of the main ingredients of the isolating agent of the present invention during the silkworm breeding process, the present invention sets up a plurality of experimental groups for comparative experiments, and the experiments are divided into three stages: the first stage is to set up a single-dose group to screen out suitable main ingredients, with Eupatorium adenophorum, Bidens pilosa, and Artemisia argyi as candidate main ingredients, experimental numbers 1-3, and lime as a control group, experimental number 4; the second stage is the proportion matching of the double-dose groups of the main ingredients, with Eupatorium adenophorum and Bidens pilosa being matched in different proportions, experimental numbers 5-7; the third stage is to add a compound group of secondary ingredients of mulberry branches, wood ash, and lime on the basis of the second stage, and refine the compounding ratio, experimental numbers 8-12.

[0057] The experimental silkworm varieties were Jingsong × Haoyue. 200 silkworms were raised in each experimental group, which was divided into 2 replicates, with 100 silkworms in each replicate. The experimental time was from collecting ants to the 5th day of cocooning. The experimental settings are shown in Table 1.

[0058] Table 1. Experimental group settings

[0059]

[0060] Through the above experiments of single-dose group, double-dose group and compound group, the different degrees of influence of the isolating agent of the present invention on silkworms were comprehensively evaluated from the growth weight of silkworms, the dryness of the silkworm bed microenvironment, the cocoon formation rate, the cocoon layer rate and the amount of lime used.

[0061] 1. Effect of isolating agent on the growth and weight of silkworms:

[0062] like Figure 1 As shown, from the perspective of the weight of a single head of silkworms in each experimental group at the fifth instar stage, the weight difference between each experimental group and the lime control group was calculated using the statistical model of analysis of variance (ANOVA). The average values ​​were not statistically significant. The silkworms in the single-dose group 3, the mugwort group, had the lowest weight, and the silkworms in the compound group 12 had the highest weight. Therefore, the experimental results show that the use of medicinal plant-derived isolating agents does not affect the growth weight of silkworms, and the silkworms' feeding of mulberry leaves is not affected by the medicinal plant isolating agents. The lowest weight in the mugwort group may be related to the strong smell of mugwort, and the weight of the compound group 12 is the highest, indicating that the silkworms have the best feeding situation when using this ratio of isolating agents.

[0063] 2. The influence of isolating agent on the silkworm bed environment:

[0064] The silkworm cocoon is the most direct microenvironment for the growth of silkworms. Moisture in the silkworm cocoon is easy to cause diseases in silkworms. A dry silkworm cocoon microenvironment is the basic guarantee for the healthy growth of silkworms. In order to find out whether the isolating agent plays an isolating and hygroscopic role, the dryness of the silkworm cocoon was qualitatively checked on the third day of the fifth instar when the silkworms were fed with a large amount of mulberry leaves. The results are as follows Figure 2As shown, the drying property of lime in the control conventional group is the best, indicating that the hygroscopic effect of lime is better than that of other experimental groups. The drying property of silkworm seats in the compound group 12 is second, indicating that the isolation agent of the present invention can achieve better drying effect under the premise of meeting the requirements of green environmental protection, and the drying property of silkworm seats in the overall compound group is also better than that in the single-agent group.

[0065] 3. The effect of isolating agent on the cocooning rate of silkworms:

[0066] The cocooning rate of silkworms can determine the success rate of silkworm breeding. The higher the cocooning rate, the higher the survival rate of silkworm larvae. It also indicates that there are fewer silkworm diseases and the environmental conditions for silkworm breeding are good. Figure 3 As shown in the figure, it can be seen that the cocoon formation rate of the compound groups 8, 9, 11, and 12 is higher than that of the control group 4 (lime), indicating that the use of plant-derived isolating agents can promote the cocoon formation rate of silkworms.

[0067] 4. The influence of isolating agent on silkworm cocoon layer rate:

[0068] The cocoon layer rate is an economic indicator for determining the price of silkworm cocoons. Generally, a cocoon layer rate of 20% is the standard for evaluating the quantity of cocoons. Figure 4 As shown, the experimental results showed that the cocoon layer rate of the lime group was the highest, followed by the compound group.

[0069] 5. Amount of lime used:

[0070] The experiment used a 1L volume cup and prepared the experimental compound according to the volume ratio, as shown in Table 1. Each experimental group raised a total of 200 silkworms. The lime group used 1L of lime for all ages, and the lime usage in the compound group was 40mL, which is 25 times that of the latter. According to the calculation of 2000 ants per gram, the lime usage for all ages is 10L, while the lime usage for all ages of the compound is only 400mL, which greatly reduces the lime usage. The lime usage of the compound greatly reduces the impact on the environment.

[0071] The above results show that from the comprehensive perspective of the growth weight of silkworms, the dryness of silkworm seats, the cocooning rate and the cocoon layer rate, the experimental data of compound group 12 are relatively better. Therefore, Eupatorium adenophorum and Bidens pilosa are suitable as the main components of the compound agent, and the most appropriate mixing ratio of the two is 1:1, which is beneficial to the healthy growth and development of silkworms.

[0072] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for illustrating the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which shall fall within the scope of the present invention to be protected. The scope of the present invention to be protected is defined by the attached claims and their equivalents.

Claims

1. A plant-based moisture-absorbing disease-preventing isolation agent for silkworms, characterized in that: The invention comprises main ingredients and auxiliary ingredients, wherein the main ingredients comprise Eupatorium adenophorum, Bidens pilosa, mulberry branch, plant ash and lime powder; the components by volume are: 10-80 parts of Eupatorium adenophorum, 10-80 parts of Bidens pilosa, 5-10 parts of mulberry branch, 1-5 parts of plant ash and 1-5 parts of lime powder; and the mixing ratio of the main ingredients to the auxiliary ingredients is 1-5:1-10.

2. A plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to claim 1, characterized in that: The main ingredients are composed of 30-60 parts of Eupatorium adenophorum, 30-60 parts of Bidens pilosa, 3-8 parts of mulberry branches, 2-5 parts of plant ash and 1-3 parts of lime powder in parts by volume.

3. A plant-based moisture-absorbing disease-preventing isolation agent for silkworms according to claim 2, characterized in that: The main ingredients are composed of 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of wood ash and 2 parts of lime powder in parts by volume.

4. A plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to any one of claims 1 to 3, characterized in that: The dosage form of the main ingredient is one of granules and powder.

5. The plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to claim 1, characterized in that: The auxiliary material ingredients are sand or soil from the place where the silkworms are raised.

6. A method for preparing a plant-based moisture-absorbing disease-preventing isolation agent for silkworms as claimed in any one of claims 1 to 5, characterized in that: At least the following steps are included: Step 1: selecting Eupatorium adenophorum, Bidens pilosa, mulberry branch, plant ash, and lime powder as main ingredients, and airing, cutting, and drying Eupatorium adenophorum, Bidens pilosa, and mulberry branch; Step 2: Grind the dried, cut and dried Eupatorium adenophorum, Bidens pilosa and Morus alba into granules or powder respectively; Step 3: sieve the ground Eupatorium adenophorum, Bidens pilosa, and Morus alba respectively, using a sieve of 50 to 300 meshes to select the desired granules or powders; Step 4: Spread out the selected granules or powders of Eupatorium adenophorum, Bidens pilosa, and Morus alba respectively and air them; Step 5: mixing the granules or powders of Eupatorium adenophorum, Bidens pilosa, and Morus alba in step 4 with plant ash and lime powder to prepare a dosage form of the main ingredient composition; Step 6: Mix the dosage form of the main ingredient composition in step 5 with the auxiliary ingredients in a ratio of 1-5:1-10 to prepare a moisture-absorbing disease-preventing isolation agent for silkworms.

7. The method for preparing a plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to claim 6, characterized in that: The drying in step 1 is performed in the midday sun for 3 hours, and the dried stems of Zedoaria, Bidens pilosa, and mulberry branches are turned over every hour. The cut length is 5 cm, and the oven is set at 65° C. for 24 hours of drying.

8. The method for preparing a plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to claim 6, characterized in that: The drying step in step 4 is to expose the product to the midday sun for 2 hours, or to use an oven set at 65° C. to dry the product for 30 minutes.

9. The method for preparing a plant-based moisture-absorbing and disease-preventing isolation agent for silkworms according to claim 6, characterized in that: The mixing proportions of the main ingredients in step 5 are as follows by volume: 45 parts of Eupatorium adenophorum, 45 parts of Bidens pilosa, 5 parts of mulberry branches, 3 parts of wood ash, and 2 parts of lime powder.

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