An antioxidant composition, a preparation method thereof, a cosmetic, and applications

Abstract

The present invention discloses an antioxidant composition, a preparation method thereof, a cosmetic, and an application. The antioxidant composition comprises the following components by weight: 0.1 - 2 parts of glutathione disulfide, 1 - 8 parts of olive extract, 0.1 - 2 parts of taurine sulfonate, 1 - 5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 5 - 10 parts of vegetable oil, 20 - 50 parts of polyol, and 23 - 72.8 parts of water. The antioxidant composition of the present invention can be stored for a long time, is not easily discolored or inactivated, has significant antioxidant and whitening effects, and has a significant inhibitory effect on superoxide anion, hydroxyl radical, DHHP, and ROS. It also has excellent antioxidant stability and can be used as an additive in skin care products.

Description

Technical Field

[0001] The present invention relates to an antioxidant composition, a preparation method thereof, a cosmetic and an application thereof. Background Art

[0002] Oxidative stress caused by the external environment can lead to cellular oxidation, thereby generating free radicals. The peroxidative damage and melanin deposition of the skin caused by free radicals are major enemies of skin aging and beauty. Making the skin antioxidant system and natural antioxidants play a better role is an important part of skin beauty. Inhibiting and scavenging free radicals is also an important way to whiten the skin.

[0003] There are various types of reactive oxygen free radicals, which are divided into oxygen free radicals, nitrogen free radicals, singlet oxygen, hydrogen peroxide (H 2 O 2 )), peroxides (LOOH), etc. Currently, antioxidants commonly used for skin antioxidant in the market include vitamin C, vitamin E, SOD (superoxide dismutase), astaxanthin, tea polyphenols, and some plant extracts, such as pomegranate extract, tea extract, grape seed extract, etc. The antioxidant ability of different antioxidants depends on the type of free radicals, the generation process, the generation site, and the target it acts on. A single antioxidant often can only play an antioxidant role against one or several types, such as vitamin E and plant extracts. There are also some antioxidants with poor stability, which are extremely easy to be oxidized by the environment, lose their antioxidant activity, and even cause discoloration problems. For example, SOD cannot be stored at room temperature; astaxanthin is easy to change color under light. Among them, glutathione is a tripeptide composed of three amino acids. Due to the presence of a mercapto group, it is the main endogenous antioxidant in cells, preventing damage by neutralizing free radicals and reactive oxygen species such as peroxides. Glutathione has two different forms: the active form of reduced glutathione (GSH) and the inactive form of oxidized glutathione (GSSG). In the prior art, reduced glutathione is generally directly used, but since it is extremely easy to be oxidized into oxidized glutathione and thus lose its antioxidant activity, it is difficult to be actually applied in the cosmetic field. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to overcome the defects in the prior art that glutathione has poor stability, is easy to discolor, is extremely easy to be oxidized and lose its antioxidant activity, thus being difficult to be actually applied, and to provide an antioxidant composition, a preparation method thereof, a cosmetic and an application thereof. The antioxidant composition of the present invention can be stored for a long time, is not easy to discolor or inactivate, has significant antioxidant and whitening effects, and has a significant inhibitory effect on superoxide anion, hydroxyl radical, DHHP and ROS, and also has excellent antioxidant stability, and can be used as an additive in skin care products.

[0005] When glutathione patrols the cellular environment and extinguishes oxidative "free radical" fires, it is itself oxidized and loses its activity, thus becoming oxidized glutathione (GSSG). The present invention directly uses oxidized glutathione to prepare an antioxidant composition, which can reactivate oxidized glutathione, effectively scavenge a variety of different free radicals, and can achieve antioxidant and whitening effects in the human body. This antioxidant composition can be used as an additive in skin care products and can be stored for a long time, capable of solving common problems of antioxidants such as discoloration and inactivation.

[0006] Specifically, during the R & D process, the inventors unexpectedly found that specific dosages of taurine, olive fruit extract and glutathione disulfide (GSSG) have synergistic antioxidant and whitening effects. Among them, taurine can decompose in the body to generate hypotaurine, which then undergoes an oxidation-reduction reaction with glutathione disulfide (GSSG), converting itself into taurine, while glutathione disulfide is converted into reduced glutathione, enabling glutathione to exert persistent activity; olive fruit extract can make the antioxidant effect of the composition more comprehensive and enhance the whitening effect of the human body.

[0007] It was also found during the R & D process that specific dosages of Candida utilis / glucose / methyl oleate fermentation products, vegetable oils, polyols and water are crucial for the stability of the antioxidant composition. The absence of any one of them will lead to instability of the system, resulting in layering or demulsification. That is, specific dosages of Candida utilis / glucose / methyl oleate fermentation products, vegetable oils, polyols, water and a specific ratio of "taurine, olive fruit extract and glutathione disulfide (GSSG)" cooperate with each other to effectively stabilize the encapsulation system, enabling the antioxidant composition of the present invention to be stored for a long time without common problems of antioxidants such as discoloration and inactivation.

[0008] The present invention solves the above technical problems through the following technical solutions:

[0009] The present invention provides an antioxidant composition, which comprises the following weight components: 0.1 - 2 parts of glutathione disulfide (GSSG), 1 - 8 parts of olive extract, 0.1 - 2 parts of taurine, 1 - 5 parts of Candida utilis / glucose / methyl oleate fermentation products, 5 - 10 parts of vegetable oil, 20 - 50 parts of polyol and 23 - 72.8 parts of water.

[0010] In the present invention, preferably, the dosage of glutathione disulfide is 0.1 - 1.5 parts, such as 0.1 part, 0.5 part, 1 part or 1.5 parts.

[0011] In the present invention, preferably, the dosage of olive extract is 1 - 6 parts, such as 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts.

[0012] In the present invention, preferably, the amount of thiotaurine is 0.1 - 1.5 parts, such as 0.1 part, 0.5 part, 1 part or 1.5 parts.

[0013] In the present invention, preferably, the mass ratio of glutathione disulfide (GSSG) to thiotaurine is 1:1 - 10:1, such as 1:1, 5:1 or 10:1.

[0014] In the present invention, preferably, the mass ratio of glutathione disulfide to olive extract is 4:1 - 10:1.

[0015] In the present invention, the total amount of glutathione disulfide, olive extract and thiotaurine is 1% - 10%, preferably 1.2% - 9%, such as 1.2%, 3%, 6.1%, 7% or 9%.

[0016] In the present invention, preferably, the amount of Candida utilis / glucose / methyl oleate fermentation product is 1 - 2.5 parts, such as 1 part, 1.5 parts, 2 parts or 2.5 parts.

[0017] In the present invention, preferably, the amount of vegetable oil is 5 parts, 6 parts, 7 parts or 8 parts.

[0018] In the present invention, preferably, the amount of polyol is 25 parts, 30 parts, 35 parts, 40 parts or 45 parts.

[0019] In the present invention, preferably, the amount of water is 50 - 60 parts, such as 50.5 parts, 52.5 parts, 56.5 parts, 58.3 parts or 58.8 parts.

[0020] In the present invention, the glutathione disulfide (GSSG) can be a conventional commercially available product in the art, for example, it can be purchased from Chengdu Jinglin Biotechnology Co., Ltd.

[0021] In the present invention, the olive extract can be a conventional olive extract in the art. Preferably, the oleuropein content in the olive extract is greater than 10%, and the hydroxytyrosol content is greater than 5%.

[0022] In the present invention, preferably, the preparation method of the olive extract includes the following steps:

[0023] Crush, dry, extract, extract, concentrate and dry the olive to obtain the olive extract.

[0024] More preferably, the preparation method of the olive extract includes the following steps:

[0025] (1) Crush the fruits and leaves of the olive, and dry and age at 120°C for 2 h;

[0026] (2) Add distilled water according to the mass ratio of the feed liquid of 1:(9 - 11) (for example, 1:10), extract at a temperature of 80 - 100 °C for 30 - 50 minutes, and obtain concentrated liquid A after concentration;

[0027] (3) Collect the remaining residue, use an alcohol solution (for example, 70% ethanol) as the solvent, according to the mass ratio of the residue to the solvent of 1:(7 - 9) (for example, 1:8), heat and reflux at a temperature of 70 - 90 °C (for example, 80 °C) for 0.5 - 1.5 h (for example, 1 h); extract with an alcohol solution (for example, n-butanol) to obtain extract B;

[0028] (4) Concentrate extract B to obtain concentrated liquid B;

[0029] (5) Mix concentrated liquid A and concentrated liquid B and perform freeze-drying at -30 to -40 °C for 2 h to obtain the olive extract.

[0030] In the present invention, the thiotaurine can be a product conventionally commercially available in the art, for example, it can be purchased from Shanghai Huiwen Biotechnology Co., Ltd.

[0031] In the present invention, the Candida sp. / glucose / methyl oleate fermentation product can be a product conventionally commercially available in the art, for example, it can be purchased from UNITED ACTIVE in the United States. The Candida sp. / glucose / methyl oleate fermentation product is a fermentation product obtained by oil fermentation, and in the present invention, it is used as a stabilizer and penetration enhancer. On the one hand, it can make the composition more stable in the formulation, and on the other hand, it is beneficial for the composition to enter the skin and exert its efficacy.

[0032] In the present invention, the vegetable oil can be a vegetable oil conventionally used in the art. Preferably, the vegetable oil is sunflower seed oil, and the sunflower seed oil can be a product conventionally commercially available in the art, for example, it can be purchased from Guangzhou Naxin Biotechnology Co., Ltd.

[0033] In the present invention, the polyol can be a polyol conventionally used in the art. Preferably, it is a polyol with a carbon chain of C 3 -C 8 and more preferably one or more of dipropylene glycol, diglycerol, propylene glycol, and butylene glycol, for example, dipropylene glycol.

[0034] In the present invention, the water can be water conventionally used in the art, generally deionized water.

[0035] In a preferred embodiment of the present invention, the antioxidant composition comprises the following weight components: glutathione disulfide (GSSG) 1 part, olive extract 5 parts, thiotaurine 1 part, Candida sp. / glucose / methyl oleate fermentation product 2.5 parts, vegetable oil 8 parts, dipropylene glycol 30 parts, and water 52.5 parts.

[0036] In another preferred embodiment of the present invention, the antioxidant composition comprises the following components by weight: 0.5 part of glutathione disulfide (GSSG), 2 parts of olive extract, 0.5 part of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseed oil acid fermentation product, 8 parts of vegetable oil, 30 parts of dipropylene glycol, and 56.5 parts of water.

[0037] In another preferred embodiment of the present invention, the antioxidant composition comprises the following components by weight: 0.1 part of glutathione disulfide (GSSG), 1 part of olive extract, 0.1 part of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseed oil acid fermentation product, 8 parts of vegetable oil, 30 parts of dipropylene glycol, and 58.3 parts of water.

[0038] In another preferred embodiment of the present invention, the antioxidant composition comprises the following components by weight: 1 part of glutathione disulfide (GSSG), 5 parts of olive extract, 0.1 part of taurine, 1 part of Candida utilis / glucose / methyl rapeseed oil acid fermentation product, 5 parts of vegetable oil, 30 parts of dipropylene glycol, and 58.8 parts of water.

[0039] In another preferred embodiment of the present invention, the antioxidant composition comprises the following components by weight: 1.5 parts of glutathione disulfide (GSSG), 6 parts of olive extract, 1.5 parts of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseed oil acid fermentation product, 10 parts of vegetable oil, 30 parts of dipropylene glycol, and 50.5 parts of water.

[0040] The present invention provides a method for preparing the antioxidant composition as described above, which comprises the following steps:

[0041] Mix the components of the antioxidant composition as described above evenly, and that's it.

[0042] In the present invention, preferably, the method for preparing the antioxidant composition comprises the following steps:

[0043] (1) Dissolve the taurine and the glutathione disulfide in water respectively to obtain an aqueous solution containing taurine and an aqueous solution containing glutathione disulfide;

[0044] Dissolve the olive extract in a polyol to obtain a solution containing the olive extract;

[0045] More preferably, in the aqueous solution containing taurine or the aqueous solution containing glutathione disulfide, the amount of water used is 9 - 11 times the weight of the solute, for example, 10 times.

[0046] (2) Mix the solution containing olive extract, the Candida utilis / glucose / methyl oleate fermentation product, and the vegetable oil evenly to obtain a Phase A mixture.

[0047] (3) While stirring, dropwise add the aqueous solution containing taurine to the Phase A mixture, and homogenize at 4000 rpm for 4 - 5 minutes.

[0048] (4) Add the aqueous solution containing glutathione disulfide and the remaining water to the Phase A mixture, and stir evenly to obtain the product.

[0049] The present invention provides a cosmetic containing the aforementioned antioxidant composition.

[0050] In the present invention, the cosmetic can be a serum, a facial mask liquid, a lotion, or a cream.

[0051] In the present invention, in the cosmetic, the dosage of the antioxidant composition can be 1 - 5%, for example, 1%. The above percentages are the weight percentages of the antioxidant composition and the cosmetic.

[0052] In the present invention, preferably, the cosmetic includes the following components: 1 - 5% of the antioxidant composition, 1 - 5% of glycerol, 0.1 - 0.5% of a thickener, 0.2 - 1.0% of a preservative, 0 - 5% of an emulsifier, and 0 - 20% of an oil, with the balance being water. The above percentages are the weight percentages of each component relative to the cosmetic respectively.

[0053] In the present invention, preferably, the serum includes the following components: 1% of the antioxidant composition, 5% of glycerol, 0.25% of a thickener, 0.25% of a preservative, and 93.5% of water. The above percentages are the weight percentages of each component relative to the serum respectively.

[0054] The present invention provides an application of the aforementioned antioxidant composition in cosmetics.

[0055] On the basis of conforming to common knowledge in the art, the above preferred conditions can be combined arbitrarily to obtain various preferred examples of the present invention.

[0056] The reagents and raw materials used in the present invention are all commercially available.

[0057] The positive and progressive effects of the present invention are as follows:

[0058] 1. The antioxidant composition of the present invention can be stored for a long time, is not easily discolored or inactivated, has significant antioxidant and whitening effects, and has a significant inhibitory effect on superoxide anion, hydroxyl radical, DHHP, and ROS. It also has excellent antioxidant stability and can be used as an additive in skin care products.

[0059] 2. The antioxidant composition prepared from the preferred combinations of the present invention can synergistically enhance each other, with the superoxide anion elimination rate reaching over 38.6%, the hydroxyl radical elimination rate reaching over 56.5%, the DHHP elimination rate reaching over 34.6%, the ROS elimination rate reaching over 40.6%, and the reduction rates of hydroxyl radicals and DHHP within 28 days being below 20%.

[0060] 3. The composition of the present invention can penetrate the skin well and has significant antioxidant and whitening effects in cosmetics with an addition amount of 1% - 5%, and the use cost is low. Detailed implementation manners

[0061] The present invention will be further described below by way of examples, but the present invention is not limited to the scope of the described examples accordingly.

[0062] The raw materials used in the following examples are as follows:

[0063] Glutathione disulfide (GSSG) was purchased from Chengdu Jinglin Biotechnology Co., Ltd., with a purity of 99%.

[0064] Thiotaurine was purchased from Shanghai Huiwen Biotechnology Co., Ltd.

[0065] Candida utilis / glucose / methyl oleate fermentation product was purchased from UNITED ACTIVE, USA.

[0066] Sunflower seed oil was purchased from Guangzhou Naxin Biotechnology Co., Ltd.

[0067] Dipropylene glycol was purchased from Dow Chemical.

[0068] Ethyl ether of vitamin C was purchased from CosMol Co., Ltd., Korea.

[0069] The olive extracts were all prepared by the following method:

[0070] 1) Crush the fruits and leaves of olives and dry and age them at 120°C for 2 h; 2) Add distilled water according to the mass ratio of material to liquid of 1:10, extract at a temperature of 80 - 100°C for 30 - 50 minutes, and then obtain concentrated solution A through rotary evaporation; 3) Collect the remaining residue, use 70% ethanol as the solvent, heat and reflux at 80°C for 1 h according to the mass ratio of residue to solvent of 1:8; then extract with n-butanol to obtain extract B; 4) Perform rotary evaporation on extract B to obtain concentrated solution B; 5) Mix concentrated solution A and concentrated solution B and perform freeze-drying at -30 to -40°C for 2 h to obtain olive extracts.

[0071] Examples 1 - 5

[0072] The formulations of the antioxidant compositions of Examples 1 - 5 are shown in Table 1.

[0073] Table 1 Formulations of Antioxidant Compositions in Examples 1-5

[0074]

[0075]

[0076] Preparation Method of Antioxidant Composition:

[0077] 1) Dissolve taurine thiosulfate and glutathione disulfide separately in deionized water equivalent to 10 times their own weights, and dissolve olive extract in dipropylene glycol for later use;

[0078] 2) Mix the dipropylene glycol solution of olive extract, Candida utilis / glucose / methyl oleate fermentation product, and sunflower seed oil evenly by stirring to obtain a phase A mixture;

[0079] 3) While stirring rapidly, add the dissolved aqueous solution of taurine thiosulfate dropwise to the phase A mixture, and homogenize at 4000 rpm for 4-5 minutes;

[0080] 4) Add the dissolved aqueous solution of glutathione disulfide to the phase A mixture and stir evenly slowly.

[0081] Comparative Examples 1-8

[0082] The formulations of the antioxidant compositions in Comparative Examples 1-4 are shown in Table 2.

[0083] Table 2 Formulations of the Antioxidant Compositions in Comparative Examples 1-4

[0084]

[0085]

[0086] The formulations of the antioxidant compositions in Comparative Examples 5-8 are shown in Table 3.

[0087] Table 3 Formulations of the Antioxidant Compositions in Comparative Examples 5-8

[0088]

[0089] The preparation methods of the antioxidant compositions in Comparative Examples 1-8 are the same as those in Example 1. Note: " / " in Tables 1-3 indicates that the substance is not added.

[0090] Effect Example 1

[0091] 1. Elimination Effect on Superoxide Anion Radical (·O 2 - )

[0092] Experimental method: Under aerobic conditions, xanthine oxidase can convert xanthine into uric acid and simultaneously generate superoxide anion radicals (·O 2 - ), so the elimination rate of superoxide anions by the sample can be detected by the chromogenic agent nitroblue tetrazolium.

[0093] Experimental procedure: Step (1): Sequentially add 100 μL of a mixed solution of xanthine (0.4 mmol / ) and NBT (0.24 mmol / l) (50 μL of each, dissolved in 0.01 mol / l PBS, pH = 8.0), 100 μL of xanthine oxidase (0.049 units / ml), and 50 μL of the sample solution (i.e., the antioxidant composition of the example or comparative example) into the wells of a 96-well plate, and incubate for 30 minutes. Step (2): Measure the OD560 value using an enzyme-linked immunosorbent assay reader. The sample solution without the antioxidant composition is used as a blank control, and only vitamin C is added as a positive control. Take the average value of three measurements and calculate the clearance rate.

[0094] Test results:

[0095] Table 4 Superoxide anion elimination rates of Examples 1-5 and Comparative Examples 1-8

[0096]

[0097] As can be seen from the above table, the antioxidant composition of the present invention can better scavenge superoxide anions. From Examples 1-5, it can be seen that the addition amounts of the three active ingredients have a greater impact on the elimination rate of superoxide anions. The superoxide anion elimination rate is above 38.6%, all greater than those of Comparative Examples 1-8. Among them, the superoxide anion elimination rate of the optimal Example 5 of the present application can reach 75.3%.

[0098] 2. Scavenging effect on hydroxyl radicals (·OH)

[0099] Experimental method: The ability to inhibit hydroxyl radicals (U / ml) was measured according to the operation instructions of the kit. The hydroxyl radical assay kit was purchased from Nanjing Jiancheng Company. Vitamin C was used as a positive control. Take the average value of three measurements and calculate the clearance rate.

[0100] Test results:

[0101] Table 5 Hydroxyl radical elimination rates of Examples 1-5 and Comparative Examples 1-8

[0102]

[0103] As can be seen from the above table, the antioxidant composition of the present invention can better scavenge hydroxyl radicals. The hydroxyl radical elimination rates of Examples 1-5 are above 56.5%, greater than those of Comparative Examples 2-8. Among them, the hydroxyl radical clearance rate of Example 1 of the present application can reach 81.5%.

[0104] 3. DPPH Radical Scavenging Rate

[0105] Experimental method: (1) Prepare a 0.1 mmol / L DPPH solution (2,2-diphenyl-1-picrylhydrazyl, commercially available from Macklin) with absolute ethanol and store it in the dark. (2) Add 2 mL of the test sample solution and 2 mL of the DPPH solution to the same test tube, shake well, and let it stand in the dark at room temperature for 30 min, then measure the absorbance at 520 nm, denoted as A sample. At the same time, measure the absorbance of the mixture of 2 mL of the DPPH solution and 2 mL of the solvent, denoted as A 0 . (3) Representation of radical scavenging ability: DPPH scavenging rate (%) = (A 0 - Asample) / A 0 * 100.

[0106] Test results:

[0107] Table 6 DPPH elimination rates of Examples 1-5 and Comparative Examples 1-8

[0108]

[0109] As can be seen from the above table, the DHHP elimination rates of Examples 1-5 are all greater than 34.6%, and are greater than those of Comparative Examples 3-5, Comparative Example 7, and Comparative Example 8.

[0110] 4. Intracellular Reactive Oxygen Species (ROS) Scavenging Rate

[0111] Experimental method: (1) Cell culture: Collect and count fibroblasts that have grown to 80-90%. According to the counting results, inoculate them into a 12-well cell culture plate at a density of 2-8×10 4 cells / well and culture overnight. (2) Use 10 mg / ml quinodimethacrylate as the positive control, and the experimental group is the 100 mg / ml sample solution. The administration time is 4 h. (3) Take the ROS detection kit (purchased from Nanjing Jiancheng), operate according to the kit requirements, detect the fluorescence intensity with an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the ROS scavenging rate.

[0112] Test results:

[0113] Table 7 ROS elimination rates of Examples 1-5 and Comparative Examples 1-8

[0114]

[0115]

[0116] As can be seen from the above table, the ROS elimination rates of Examples 1-5 are 40.6-56.2, and are all greater than those of Comparative Examples 1-8.

[0117] 5. Whitening Human Body Efficacy Test

[0118] Experimental method: The essence solutions of the above Examples 1-5 and Comparative Examples 1-8 were respectively formulated into essence solutions according to the following formula, and 156 volunteers aged between 25 and 55 were selected for product trial experiments. The volunteers were evenly divided into 13 groups, with 12 people in each group.

[0119] The essence solution was prepared according to the following formula:[[]]END]]

[0120] Table 8 Essence Solution Formula

[0121] Raw material name Commercially available source Addition amount % Deionized water / 93.5 Glycerol Spring Gold 5.0 Xanthan gum C.P. Kelco 0.25 Preservative GPL Lonza 0.25 Antioxidant composition / 1.0

[0122] For the 1st-5th groups, the essence solutions prepared by adding Examples 1-5 were respectively used for trial; for the 6th-13th groups, the essence solutions prepared by adding Comparative Examples 1-8 were respectively used for trial.

[0123] It is required that the above-mentioned trial users apply the product in the size of a soybean (about 0.5 ml) to the face twice a day, once in the morning and once in the evening, after cleaning the face, and gently massage until absorbed. Taking 7 days as a cycle, the trial data of the volunteers were respectively counted after 7 days, 14 days, and 28 days.

[0124] Testing Instruments and Parameters:

[0125] Table 9 Testing Instruments and Parameters for Whitening Human Body Efficacy Test

[0126]

[0127]

[0128] Statistical Results:

[0129] Table 10 ITA° Values of Skin Whiteness and MI Values of Melanin in the 1st-13th Groups

[0130]

[0131] As can be seen from the above table, the improvement rates of the ITA° values of skin whiteness and MI values of melanin of the trial users of the essence solutions prepared by Examples 1-5 on the 7th day, 14th day, and 28th day are all greater than those of the trial users of the essence solutions of Comparative Examples 1-8.

[0132] 6. Statistical Results

[0133] The results of the above-mentioned efficacy tests in items 1-5 are as follows:

[0134] Table 11 Efficacy Test Results of the 1st-13th Groups

[0135]

[0136]

[0137] As can be seen from the above table, the antioxidant composition of the present invention can effectively scavenge free radicals and has the effect of whitening the human body. The superoxide anion radical, hydroxyl radical, DPPH radical, intracellular reactive oxygen species (ROS), and the effect of whitening the human body in Groups 1-5 are all significantly effective or effective, while only a few in Groups 6-13 are significantly effective, and most are not significant or effective.

[0138] It can be seen from Groups 1-5 that the addition amounts of the three active ingredients have a great influence on the performance of the antioxidant composition; when the addition amounts of the three active ingredients are 1.2%-9%, the antioxidant composition can not only effectively scavenge superoxide anion radicals, hydroxyl radicals, DPPH radicals, and intracellular reactive oxygen species (ROS), but also has excellent whitening effect on the human body; although under the same composition, the contents of the three active ingredients in Comparative Example 7 and Comparative Example 8 are also relatively high, but the proportion of glutathione disulfide and taurine in Comparative Example 7 (Group 12) is only 0.05%, and the proportion of olive extract in Comparative Example 8 (Group 13) is only 0.5%. Therefore, except that the effect on the elimination rate of hydroxyl radicals in Group 12 is effective, the elimination rates of free radicals and the whitening effect in Groups 12 and 13 are not significant. In Comparative Example 1 (Group 6), the three active ingredients are 7%, but Candida utilis / glucose / methyl oleate fermentation product and vegetable oil are not added, and its whitening effect is not significant; in Comparative Example 2 (Group 7), taurine is not added, and the elimination effects of superoxide anion radicals and intracellular reactive oxygen species (ROS) and the whitening effect on the human body are not significant; in Comparative Example 3 (Group 8), glutathione disulfide is not added, and its free radical elimination rate and whitening effect on the human body are not significant; in Comparative Example 4 (Group 9), olive extract is not added, and its free radical elimination rate and whitening effect on the human body are not significant; in Comparative Example 5 (Group 10), glutathione disulfide and taurine are not added, and except for the significant effect on DPPH, the elimination effects on other free radicals and the whitening effect on the human body are not significant; in Comparative Example 6 (Group 11), the three active components are replaced with vitamin C ethyl ether, although it has an elimination effect on some free radicals, its whitening effect is not significant. Therefore, within the range of each component of the present application, the higher the content of the three active ingredients, the better the free radical scavenging and whitening effects, and the lack of a certain component cannot ensure that while effectively scavenging free radicals, it also has a whitening effect.

[0139] Effect Example 2

[0140] 1. Investigate the antioxidant stability of the samples during the shelf life (hydroxyl radical (·OH) and DPPH tests)

[0141] Examples 1-4, Comparative Example 1, and Comparative Example 5 were respectively placed in an incubator at 45 °C for accelerated stability testing. The scavenging rates of hydroxyl radicals (·OH) and DPPH were measured on the 0th day, 7th day, 14th day, and 28th day respectively.

[0142] (1) Test data for the scavenging rate of hydroxyl radicals:

[0143] Table 12 Test data for the scavenging rate of hydroxyl radicals of Examples 1-4, Comparative Example 1, and Comparative Example 5

[0144] Sample solution Day 0 Day 7 Day 14 Day 28 Reduction rate at 28 days Example 1 81.5％ 80.0％ 78.8％ 77.2％ 5.28％ Example 2 73.8％ 71.2％ 69.3％ 66.7％ 9.62％ Example 3 56.5％ 54.8％ 53.8％ 52.4％ 7.26％ Example 4 58.2％ 55.2％ 52.6％ 49.7％ 14.60％ Comparative Example 1 64.7％ 47.0％ 36.4％ 18.0％ 72.18％ Comparative Example 5 23.2％ 16.1％ 10.3％ 3.06％ 86.81％

[0145] The reduction rate of the hydroxyl radical scavenging rate of Examples 1-4 within 28 days was less than 14.6%, much lower than that of Comparative Example 1 and Comparative Example 5. This indicates that the antioxidant composition of the present application has good stability in the scavenging rate of hydroxyl radicals within 28 days. Although the elimination rate of hydroxyl radicals of Comparative Example 1 without adding Candida utilis / glucose / methyl rapeseed oil fatty acid fermentation product and vegetable oil was very high on the 0th day, after 28 days, the reduction rate was 72.18%. The hydroxyl radical scavenging rate of Comparative Example 5 without adding the three active ingredients was only 23.2% on the 0th day, and the reduction rate reached 86.81% on the 28th day.

[0146] (2) Test data for the scavenging rate of DPPH radicals:

[0147] Table 13 Test data for the scavenging rate of DPPH radicals of Examples 1-4, Comparative Example 1, and Comparative Example 5

[0148] Sample solution Day 0 Day 7 Day 14 Day 28 Reduction rate at 28 days Example 1 70.6％ 68.7％ 69.2％ 67.5％ 4.39％ Example 2 65.3％ 62.1％ 59.8％ 56.5％ 13.5％ Example 3 34.6％ 35.4％ 33.7％ 31.0％ 10.4％ Example 4 48.5％ 47.8％ 44.6％ 39.3％ 19.0％ Comparative Example 1 53.5％ 36.0％ 23.7％ 21.4％ 60.0％ Comparative Example 5 32.5％ 13.4％ 6.8％ 2.6％ 92.0％

[0149] The reduction rate of the DPPH radical scavenging rate of Examples 1-4 within 28 days was less than 19.0%, much lower than that of Comparative Example 1 and Comparative Example 5. This indicates that the antioxidant composition of the present application has good stability in the scavenging rate of DPPH radicals within 28 days. Although the elimination rate of DPPH radicals of Comparative Example 1 without adding Candida utilis / glucose / methyl rapeseed oil fatty acid fermentation product and vegetable oil was relatively high compared with Examples 3 and 4 on the 0th day, after 28 days, the reduction rate was 60.0%. The hydroxyl radical scavenging rate of Comparative Example 5 without adding the three active ingredients was only 32.5% on the 0th day, and the reduction rate was as high as 92.0% on the 28th day.

[0150] Based on the test data of the scavenging rates of the above two radicals, it can be obtained that Examples 1-4 have good stability in the scavenging rate of radicals during the one-month investigation period, while the reduction values of the scavenging rates of radicals of Comparative Example 1 and Comparative Example 5 exceed 50% within one month, showing poor stability.

[0151] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example, and the protection scope of the present invention is defined by the claims. Without departing from the principle and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. An antioxidant composition, characterized in that, it comprises the following components by weight: 0.5 - 2 parts of glutathione disulfide, 1 - 8 parts of olive extract, 0.1 - 2 parts of taurine, 1 - 5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 5 - 10 parts of vegetable oil, 20 - 50 parts of polyol, and 23 - 72.8 parts of water; The preparation method of the olive extract comprises the following steps: (1) Crush the fruits and leaves of olives and dry and age them at 120°C for 2 h; (2) Add water according to the mass ratio of material to liquid of 1:(9 - 11), extract at a temperature of 80 - 100°C for 30 - 50 minutes, and concentrate to obtain concentrated liquid A; (3) Collect the remaining residue, use an alcohol solvent as the solvent, and heat and reflux at a mass ratio of residue to solvent of 1:(7 - 9) at a temperature of 70 - 90°C for 0.5 - 1.5 h; extract with an alcohol solvent to obtain extract B; (4) Concentrate extract B to obtain concentrated liquid B; (5) Mix concentrated liquid A and concentrated liquid B and perform freeze-drying at -30~-40°C for 2 h to obtain olive extract.

2. The antioxidant composition according to claim 1, characterized in that, the dosage of glutathione disulfide is 0.5 - 1.5 parts; and / or, the dosage of olive extract is 1 - 6 parts; and / or, the dosage of taurine is 0.1 - 1.5 parts; and / or, the dosage of Candida utilis / glucose / methyl rapeseedate fermentation product is 1 - 2.5 parts; and / or, the dosage of vegetable oil is 5 parts, 6 parts, 7 parts or 8 parts; and / or, the dosage of polyol is 25 parts, 30 parts, 35 parts, 40 parts or 45 parts; and / or, the dosage of water is 50 - 60 parts.

3. The antioxidant composition according to claim 2, characterized in that, the dosage of glutathione disulfide is 0.5 parts, 1 part or 1.5 parts; and / or, the dosage of olive extract is 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts; and / or, the dosage of taurine is 0.1 part, 0.5 part, 1 part or 1.5 parts; and / or, the dosage of Candida utilis / glucose / methyl rapeseedate fermentation product is 1 part, 1.5 parts, 2 parts or 2.5 parts; and / or, the dosage of water is 50.5 parts, 52.5 parts, 56.5 parts, 58.3 parts or 58.8 parts.

4. The antioxidant composition according to claim 1, characterized in that, the mass ratio of glutathione disulfide to taurine is 1:1 - 10:1; or, the total dosage of glutathione disulfide, olive extract and taurine is 1% - 10%.

5. The antioxidant composition according to claim 4, characterized in that, the mass ratio of glutathione disulfide to taurine is 1:1, 5:1 or 10:1; or, the total dosage of glutathione disulfide, olive extract and taurine is 1.2% - 9%.

6. The antioxidant composition according to claim 5, characterized in that, The total dosage of glutathione persulfide, the olive extract and taurine is 1.2%, 3%, 6.1%, 7% or 9%.

7. The antioxidant composition according to claim 1, characterized in that the oleuropein content in the olive extract is greater than 10%, and the hydroxytyrosol content is greater than 5%.

8. The antioxidant composition according to claim 1, characterized in that The polyol has a carbon chain of C 3 -C 8 and is a polyol; and / or, the water is deionized water.

9. The antioxidant composition according to claim 8, characterized in that the polyol is one or more of dipropylene glycol, diglycerol, propylene glycol and butylene glycol.

10. The antioxidant composition according to claim 9, characterized in that the polyol is dipropylene glycol.

11. The antioxidant composition according to claim 10, characterized in that the antioxidant composition comprises the following weight components: 1 part of glutathione persulfide, 5 parts of olive extract, 1 part of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 8 parts of vegetable oil, 30 parts of dipropylene glycol and 52.5 parts of water; or, the antioxidant composition comprises the following weight components: 0.5 part of glutathione persulfide, 2 parts of olive extract, 0.5 part of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 8 parts of vegetable oil, 30 parts of dipropylene glycol and 56.5 parts of water; or, the antioxidant composition comprises the following weight components: 1 part of glutathione persulfide, 5 parts of olive extract, 0.1 part of taurine, 1 part of Candida utilis / glucose / methyl rapeseedate fermentation product, 5 parts of vegetable oil, 30 parts of dipropylene glycol and 58.8 parts of water; or, the antioxidant composition comprises the following weight components: 1.5 parts of glutathione persulfide, 6 parts of olive extract, 1.5 parts of taurine, 2.5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 10 parts of vegetable oil, 30 parts of dipropylene glycol and 50.5 parts of water.

12. A method for preparing the antioxidant composition according to any one of claims 1 to 11, characterized in that it comprises the following steps: (1) Dissolve the taurine and the glutathione persulfide in water respectively to obtain an aqueous solution containing taurine and an aqueous solution containing glutathione persulfide; Dissolve the olive extract in the polyol to obtain a solution containing the olive extract; (2) Mix the solution containing the olive extract, the Candida utilis / glucose / methyl rapeseedate fermentation product and the vegetable oil evenly to obtain a phase A mixture; (3) While stirring, dropwise add the aqueous solution containing taurine into the phase A mixture, and homogenize at 4000 rpm for 4 - 5 minutes; (4) Add the aqueous solution containing glutathione persulfide and the remaining water into the phase A mixture, and stir evenly to obtain the product.

13. The method for preparing the antioxidant composition according to claim 12, characterized in that in the aqueous solution containing taurine or the aqueous solution containing glutathione persulfide, the dosage of water is 9 - 11 times the weight of the solute.

14. A cosmetic product comprising the antioxidant composition according to any one of claims 1 to 11.

15. The cosmetic product according to claim 14, wherein, the cosmetic product is a serum, a facial mask liquid, a lotion or a cream; and / or, in the cosmetic product, the dosage of the antioxidant composition is 1-5%, and the above percentage is the weight percentage of the antioxidant composition and the cosmetic product; and / or, the cosmetic product comprises the following components: 1-5% of the antioxidant composition, 1-5% of glycerol, 0.1-0.5% of a thickener, 0.2-1.0% of a preservative, 0-5% of an emulsifier and 0-20% of an oil, and the balance is water, and the above percentages are the weight percentages of the respective components relative to the cosmetic product.

16. The cosmetic product according to claim 15, wherein, in the cosmetic product, the dosage of the antioxidant composition is 1%, and the above percentage is the weight percentage of the antioxidant composition and the cosmetic product; and / or, the serum comprises the following components: 1% of the antioxidant composition, 5% of glycerol, 0.25% of a thickener, 0.25% of a preservative and 93.5% of water, and the above percentages are the weight percentages of the respective components relative to the serum.

17. Use of an antioxidant composition according to any one of claims 1 to 11 in the preparation of a cosmetic product.

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