A composite fermentation product, a composition with anti-aging efficacy and its application

Abstract

The present invention relates to a composition with anti-aging efficacy and its application. The hydrolyzed Candida strain after activation culture is inoculated into a mixed oil of Alnus rhombifolia seed oil and the fermentation product of Candida apicola / glucose / methyl rapeseedate, and fermentation is carried out to obtain a preliminary fermentation product; the obtained preliminary fermentation product is centrifuged and filtered to obtain a composite fermentation product containing various metabolites. The composition with anti-aging efficacy includes the composite fermentation product, bakuchiol, and rice fermentation broth, and the weight ratio of the composite fermentation product, bakuchiol, and rice fermentation broth is 1-3:1-2:1-3. Compared with the prior art, the present invention can form a self-assembled reverse micelle system, reducing the harm of emulsifiers to the skin. The synergistic effect of the composite fermentation product, bakuchiol, and rice fermentation broth in the composition with anti-aging efficacy jointly exerts the anti-aging efficacy.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemical products, and particularly relates to a composite fermentation product, a composition with anti-aging efficacy and its application. Background Art

[0002] Currently, many commercially available anti-aging cosmetic creams are of the water-in-oil system, with poor moisturizing effect, low stability, poor brightness of the paste and poor moisturizing degree after use. And many currently commercially available oil-in-water system creams have a very greasy and heavy feel when applied.

[0003] In traditional anti-aging cosmetics, emulsifiers are usually added to ensure the stability of the system. However, for aging skin, its metabolism decreases and the active enzymes in the body decrease. The emulsifiers added in cosmetics will cause damage and burden to aging skin. Summary of the Invention

[0004] Based on the problem that emulsifiers in anti-aging cosmetics in the prior art will cause damage and burden to aging skin, the present invention provides a composite fermentation product, a composition with anti-aging efficacy and its application.

[0005] The object of the present invention can be achieved by the following technical solutions:

[0006] The first aspect of the present invention provides a composite fermentation product, and the preparation method is as follows:

[0007] (1) Strain activation: Activate and culture the Candida bombicola strain.

[0008] (2) Fermentation: Inoculate the activated Candida bombicola strain into the mixed oil of Alnus rhombifolia seed oil and the fermentation product of Candida apicola / glucose / methyl oleate, and carry out fermentation to obtain a preliminary fermentation product.

[0009] (3) Purification: Centrifuge and filter the preliminary fermentation product obtained in step (2) to obtain a composite fermentation product containing various metabolites.

[0010] In step (2), the active ingredients in the fermentation product of Candida apicola / glucose / methyl oleate will be dissolved and extracted into the Alnus rhombifolia seed oil.

[0011] In one embodiment of the present invention, in step (1), the method for activating and culturing the Candida bombicola strain is as follows: Take the Candida bombicola strain on a clean bench, use a sterile inoculation spatula to cut the bacterial slope into small pieces, take a small piece and inoculate it on a petri dish for activation and culturing. The petri dish is filled with PDA solid medium (with a dosage of 25 ml), the culturing temperature is 30 °C, and the culturing time is 7 d. The Candida bombicola strain described in step (1) is a commercially available Candida bombicola strain.

[0012] In one embodiment of the present invention, in step (2), the weight ratio of the Alnus rhombifolia seed oil and the fermentation product of Candida apicola / glucose / methyl oleate is 1-2:1-2. In one embodiment of the present invention, in step (2), the conditions for fermentation are as follows: the inoculum size is 5 vol%-20 vol%, the culturing temperature is 20 °C-50 °C, the stirring speed is 50 rpm-200 rpm, and the fermentation time is 12-24 h. Preferably, in step (2), the conditions for fermentation are as follows: the inoculum size is 10 vol%, the culturing temperature is 30 °C, the stirring speed is 150 rpm, and the fermentation time is 24-48 h. The inoculum size is the volume ratio of the activated and cultured Candida bombicola strain to the mixed oil.

[0013] In one embodiment of the present invention, in step (3), the conditions for centrifugation are as follows: Centrifugation is carried out at a rotational speed of 6000-10000 rpm (for example, 8000 rpm).

[0014] In one embodiment of the present invention, in step (3), the metabolite includes organic acids, esters, vitamins, etc.

[0015] The second aspect of the present invention provides a composition with anti-aging efficacy, including the composite fermentation product provided by the first aspect of the present invention, as well as bakuchiol and rice fermentation broth, wherein the weight ratio of the composite fermentation product, bakuchiol and rice fermentation broth is 1-3:1-2:1-3, preferably 2:1:2.

[0016] The third aspect of the present invention provides a preparation method of the composition with anti-aging efficacy. Add bakuchiol and rice fermentation broth to the composite fermentation product provided by the first aspect of the present invention, and stir evenly to obtain the composition with anti-aging efficacy.

[0017] The fourth aspect of the present invention provides the application of a composition with anti-aging efficacy, wherein the composition with anti-aging efficacy is added to cosmetics or skin care products for the preparation of cosmetics or skin care products. In the fourth aspect, a cosmetic or skin care product with anti-aging efficacy is also provided, including the composition with anti-aging efficacy.

[0018] The fifth aspect of the present invention provides a cream with anti-aging efficacy, containing the composition with anti-aging efficacy provided by the second aspect of the present invention.

[0019] In one embodiment of the present invention, the cream with anti-aging efficacy contains the following components in weight percentages based on a total of 100%:

[0020] Composition with anti-aging efficacy 0.5 - 5%

[0021] Humectant 15 - 30%

[0022] Thickener 5 - 10%

[0023] Antioxidant 1 - 2%

[0024] Chelating agent: 0.1 - 0.5%

[0025] Preservative: 0.1 - 0.5%

[0026] Water balance.

[0027] In one embodiment of the present invention, the humectant is selected from one or a combination of several of squalane, isopropyl myristate, glycerol, 1,3 - butanediol.

[0028] In one embodiment of the present invention, the thickener is selected from one or a combination of several of cyclopentasiloxane, carbomer.

[0029] In one embodiment of the present invention, the antioxidant is selected from tocopherol.

[0030] In one embodiment of the present invention, the chelating agent is selected from disodium ethylenediaminetetraacetate.

[0031] In one embodiment of the present invention, the preservative is selected from phenoxyethanol.

[0032] In one embodiment of the present invention, the cream with anti-aging efficacy contains the following components in weight percentages based on a total of 100%:

[0033] Humectant: Squalane 3.5%

[0034] Humectant: Isopropyl myristate 0.3%

[0035] Thickener: Cyclopentasiloxane 7.0%

[0036] Humectant: Glycerol 13.5%

[0037] Antioxidant: Tocopherol 1.0%

[0038] Humectant: 1,3 - Butanediol 3.0%

[0039] Thickener: Carbomer 0.4%

[0040] Chelating agent: Disodium EDTA 0.2%

[0041] Preservative: Phenoxyethanol 0.1%

[0042] Water q.s.

[0043] In the sixth aspect of the present invention, there is provided a method for preparing the cream having anti - aging efficacy, comprising the following steps:

[0044] (1) Dissolve carbomer and place it in a constant - temperature water bath;

[0045] (2) Heat and stir squalane, isopropyl myristate, and cyclopentasiloxane until completely melted, and continue stirring after complete melting;

[0046] (3) Heat and stir water, glycerol, tocopherol, 1,3 - butanediol, and disodium EDTA until completely melted, and continue stirring after complete melting;

[0047] (4) Add the product of (3) to the product of (1), stir, then add the product of (2), and then continue stirring;

[0048] (5) After the product of (4) is naturally cooled, add phenoxyethanol and continue stirring and cooling to room temperature;

[0049] (6) Add the composition having anti - aging efficacy to the product of (4), stir, and let stand.

[0050] Preferably, step (3) and step (2) are carried out simultaneously.

[0051] In an embodiment of the present invention, the method for preparing the cream having anti - aging efficacy comprises the following steps:

[0052] (1) Dissolve carbomer and place it in a constant - temperature water bath at 85°C;

[0053] (2) Heat squalane, isopropyl myristate, and cyclopentasiloxane to 70 - 75 °C, stir until completely melted, and continue stirring for 30 min after complete melting; (3) Heat water, glycerol, tocopherol, 1,3 - butanediol, and disodium ethylenediaminetetraacetate to 70 - 75 °C, stir until completely melted, and continue stirring for 30 min after complete melting;

[0054] (4) Add the product of (3) to the product of (1), stir for 5 min, then add the product of (2), and continue stirring for 30 min;

[0055] (5) Naturally cool the product of (4) to 50 °C, add phenoxyethanol, and continue stirring and cooling to room temperature;

[0056] (6) Add the anti - aging composition to the product of (4), stir for 30 min, and let it stand for 24 h to obtain the cream.

[0057] Preferably, steps (2) and (3) are carried out simultaneously.

[0058] In the present invention, Pentaclethra Macroloba seed oil is a liquid oil, close to human natural sebum, with a relatively high degree of unsaturation. It can quickly penetrate the skin barrier and penetrate into the subcutaneous tissue on the skin, moisturize and keep the skin moist, and has a good effect of softening and moisturizing the skin. At the same time, the composition and proportion of unsaturated fatty acids in Pentaclethra Macroloba seed oil can better promote the absorption of oils and nutrients, and form a natural protective film on the skin to prevent the loss of moisture and nutrients. Pentaclethra Macroloba seed oil can be sourced from commercially available products.

[0059] The fermentation product of Candida apicola / glucose / methyl oleate can enable the collagen composition to penetrate deep into the skin, improve the permeability of the collagen composition, regulate the water - oil balance of the skin, soften the cutin, improve the skin's absorption capacity for collagen, maintain cell elasticity, promote the regeneration of damaged tissues, improve the skin's repair ability, delay cell aging, thereby achieving the maintenance of skin elasticity, improving the skin's anti - aging ability, and moreover, it can better improve the skin's antibacterial property and reduce skin sensitivity. The fermentation product of Candida apicola / glucose / methyl oleate is sourced from commercially available products.

[0060] The composite fermentation product of the present invention is the product obtained by the mixed fermentation of Pentaclethra Macroloba seed oil and the fermentation product of Candida apicola / glucose / methyl oleate, containing various metabolites such as organic acids, esters, and vitamins, providing cell energy, delaying cell aging, and improving skin firmness and elasticity. After adding an alcohol solution (such as 1,3 - butanediol) to the composite fermentation product, a self - assembled reverse micelle system is formed, improving the stability and efficacy of the active ingredients.

[0061] Bakuchiol is a coumarin component isolated from plants and has estrogen-like effects. Bakuchiol can upregulate the expression of Co1Ⅰ, Co1Ⅲ, TIMP-1, and TIMP-2 in human skin fibroblasts, promote the expression of collagen and matrix metalloproteinase (MMPs) inhibitors, reduce the mRNA expression of MMPs, and decrease collagen degradation. In addition, bakuchiol has a certain protective effect on skin photoaging caused by ultraviolet rays. Compared with retinol, bakuchiol has higher mildness, lower irritation to the skin, and no photosensitivity, so it can be used during the day. Bakuchiol can be sourced from commercially available products.

[0062] Rice fermentation broth (INCI name: Saccharomyces cerevisiae / Rice Ferment Filtrate) is a natural rice fermentation product rich in various active ingredients such as γ-oryzanol, biological polysaccharides, and amino acids. It has the effects of activating glutathione reductase, whitening, inhibiting melanin growth, and inhibiting tyrosinase activity, promotes the production of cholesterol, ceramide, and sebum, improves the softness of the skin, and has antioxidant, conditioning, skin revitalizing, and anti-aging effects. The rice fermentation broth can be sourced from self-made or commercially available products. Preferably, the rice fermentation broth meets the requirements of the group standard T / SHRH 049-2023 "Raw Materials for Cosmetics - Saccharomyces cerevisiae Rice Ferment Filtrate".

[0063] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0064] 1. The present invention selects Alnus rhombifolia seed oil and Candida apicola / glucose / methyl rapeseedate fermentation product, and uses natural oil fermentation technology to obtain a composite fermentation product. Adding bakuchiol and rice fermentation broth to the composite fermentation product and stirring evenly gives a composition with anti-aging effects. Adding the composition with anti-aging effects to cosmetics or skin care products can obtain anti-aging cosmetics or skin care products.

[0065] First, the active ingredients in the Candida apicola / glucose / methyl rapeseedate fermentation product are dissolved and extracted into the Alnus rhombifolia seed oil by means of microbial fermentation to obtain a composite fermentation product;

[0066] Second, when preparing cosmetics or skin care products, adding bakuchiol, rice fermentation broth, and an alcohol solution (such as 1,3-butanediol) to the composite fermentation product will trigger the formation of a self-assembled reverse micelle system, which will then encapsulate the active ingredients bakuchiol, rice fermentation broth, and composite fermentation product into the reverse micelles, improving the stability and efficacy of the active ingredients composite fermentation product, bakuchiol, and rice fermentation broth, and promoting the penetration of the active ingredients deep into the skin.

[0067] Again, since the present invention adopts a microbial fermentation technology, the polarity of the composite fermentation product is increased, the surface tension is reduced, the interface existing at the gaps of the horny cells is broken, and it can quickly penetrate into the deep layer of the skin, so the permeability of the active ingredients can be enhanced.

[0068] 2. The self-assembled reverse micelle system formed by the present invention maintains the stability of the system without adding an emulsifier. The active ingredients in the fermentation product of Candida apicola / glucose / methyl oleate are dissolved and extracted into the oil of Alnus grandis by means of microbial fermentation. Subsequently, free fatty acids generated during the fermentation process are separated and purified to reduce its acidity, enhance its antioxidant capacity and stability, and reduce the harm of the emulsifier to the skin.

[0069] 3. In the present invention, the composite fermentation product, bakuchiol, and rice fermentation broth in the composition with anti-aging effects act synergistically. Bakuchiol has excellent anti-aging effects. In addition to its own effects, the rice fermentation broth also has the effect of softening the skin and softening the cutin layer to promote absorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0070] Figure 1 Count of skin wrinkle (under eyes) characteristics before and after using Example 1;

[0071] Figure 2 Number of skin wrinkles (outer canthus) before and after using Example 1;

[0072] Figure 3 Skin cutin layer moisture content before and after using Example 1;

[0073] Figure 4 Skin dermis layer thickness before and after using Example 1;

[0074] Figure 5 Skin dermis layer density before and after using Example 1; DETAILED DESCRIPTION OF THE INVENTION

[0075] The raw materials in the following specific examples are all commercially available unless otherwise specified.

[0076] The Candida bombicola strain was purchased from Ningbo Mingzhou Biotechnology Co., Ltd. (B80544).

[0077] The present invention will be described in detail below with reference to the accompanying drawings and specific examples.

[0078] The composite fermentation product of the present invention is prepared by the following method:

[0079] (1) Strain activation: Take the Candida bombicola strain on the ultra-clean workbench. Use a sterile inoculation spatula to cut the bacterial slant into small pieces, and take a small piece to inoculate on a petri dish for activation culture. The petri dish contains 25 ml of PDA solid medium, the culture temperature is 30 °C, and the culture time is 12 h.

[0080] (2) Fermentation: Inoculate the activated strain into a mixed oil (mass ratio 1:1) of Alnus rhombifolia seed oil and the fermentation product of Candida apicola / glucose / methyl oleate. The culture temperature is 30 °C, the stirring speed is 150 rpm, the inoculation amount is 10 vol%, and the fermentation time is 48 h to obtain a preliminary fermentation product.

[0081] (3) Purification: Centrifuge the obtained preliminary fermentation product at a speed of 8000 rpm, and filter to obtain a composite fermentation product containing various metabolites.

[0082] Examples 1-5 and Comparative Examples 1-7

[0083] The following Examples 1-5 and Comparative Examples 1-7 provide facial creams containing different ingredients, and their compositions are shown in Table 1:

[0084] Table 1 Raw material composition formula table of Examples 1-5 and Comparative Examples 1-7

[0085]

[0086] Among them, the compositions of Component D of Examples 1-5 and Comparative Examples 1-7, which are compositions with anti-aging effects, are shown in Table 2 (each data in Table 2 represents the mass ratio relationship among the composite fermentation product, bakuchiol, and rice fermentation broth).

[0087] Table 2 Compositions of Component D of Examples 1-5 and Comparative Examples 1-6, which are compositions with anti-aging effects

[0088]

[0089]

[0090] The preparation process flow of the facial creams in the above examples is as follows:

[0091] (1) Dissolve carbomer and place it in a constant temperature water bath at 85 °C;

[0092] (2) Heat squalane, isopropyl myristate, and cyclopentasiloxane to 70-75 °C, stir until completely melted, and continue stirring for 30 min after complete melting;

[0093] (3) Heat water, glycerol, tocopherol, 1,3 - butanediol, and disodium ethylenediaminetetraacetate to 70 - 75 °C, stir until completely melted, and continue stirring for 30 min after complete melting;

[0094] (4) Add the product of (3) to the product of (1), stir for 5 min, then add the product of (2), and continue stirring for 30 min;

[0095] (5) Naturally cool the product of (4) to 50 °C, then add phenoxyethanol, and continue stirring and cooling to room temperature;

[0096] (6) Add the components of item D to the product of (4), stir for 30 min, and let stand for 24 h to obtain the cream.

[0097] The preparation process flow of the cream in the above comparative examples is as follows:

[0098] (1) Dissolve carbomer and place it in an 85 °C constant temperature water bath;

[0099] (2) Heat squalane, isopropyl myristate, and cyclopentadimethylsiloxane to 70 - 75 °C, stir until completely melted, and continue stirring for 30 min after complete melting;

[0100] (3) Heat water, glycerol, tocopherol, 1,3 - butanediol, and disodium ethylenediaminetetraacetate to 70 - 75 °C, stir until completely melted, and continue stirring for 30 min after complete melting;

[0101] (4) Add the product of (3) to the product of (1), stir for 5 min, then add the product of (2), and continue stirring for 30 min;

[0102] (5) Naturally cool the product of (4) to 50 °C, then add phenoxyethanol, and continue stirring and cooling to room temperature;

[0103] (6) Add the components of item D (if the dosage of a certain component or several components is 0, do not add) to the product of (4), stir for 30 min, and let stand for 24 h to obtain the cream.

[0104] Performance test examples

[0105] 1. Safety test

[0106] Test samples: Test substance group: creams of Examples 1 - 5; Control group: distilled water;

[0107] Test method: Use a third - party testing agency to test safety. Select an area not exceeding 50 mm 2, A suitable patch tester with a depth of about 1 mm. Using the closed patch test method, add about 0.02 - 0.025 ml (g) of the test substance or distilled water to the patch tester. Apply the patch tester to the flexor side of the subject's forearm. After 24 hours, remove the patch tester. Observe the skin reaction at 0.5 hours, 24 hours, and 48 hours after removal respectively, and record the results according to the skin reaction grading standard in "Cosmetics Safety and Technical Regulations 2015".

[0108] Table 3 Patch Test Results

[0109]

[0110] Note: Skin reaction grading standard;

[0111] Grade 0: Negative reaction;

[0112] Grade 1: Suspected reaction; Only faint erythema;

[0113] Grade 2: Weak positive reaction (erythema reaction); Erythema, infiltration, edema, and may have papules;

[0114] Grade 3: Strong positive reaction (herpes reaction); Erythema, infiltration, edema, papules, herpes; The reaction may extend beyond the test area;

[0115] Grade 4: Extremely strong positive reaction (confluent herpes reaction); Obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0116] As can be seen from Table 3 above, the prepared facial cream shows no irritation and has passed the safety test.

[0117] 2. Stability Test

[0118] Test samples: Facial creams of Examples 1 - 5;

[0119] Test method: Internal control standard, observe the appearance color, viscosity, and pH for three months.

[0120] Table 4 Stability Investigation Results

[0121] Time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE Light 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0122] As can be seen from Table 4 above, for the prepared facial cream, within three months under high and low temperatures or light, there are no significant changes in its color, viscosity, and pH, indicating that the facial creams of Examples 1 - 5 have good stability.

[0123] Test the anti - wrinkle efficacy of the facial cream

[0124] The research subjects were 110 Chinese healthy women with sensitive skin (10 people in each group, 11 groups), aged between 25 and 50 years old, with an average age of 38.50. The subjects gave written consent after being informed. The subjects were willing to cooperate and understand the necessity and duration of the control in order to comply with the protocol formulated by the clinical trial center. It was used once in the morning and once in the evening every day. About the amount of a soybean grain-sized test sample was taken with fingertips and evenly applied to the face (the whole face) and gently massaged until absorbed. It was used continuously for 28 days. At D0 (before applying the sample), D14, and D28, the experimenter used VISIA and Primos CR to detect facial images and the collagen probe Dermlab Combo to detect the skin dermis layer. The data were statistically analyzed using SPSS 28.0. The normal distribution test was performed on the test data. If the test data were normally distributed, the T-test method was used for statistical analysis; if the test data were not normally distributed, the rank sum test method was used for analysis. The rank sum test was used for statistical analysis of the rank data. The significance level of the statistical method was p < 0.05.

[0125] Test Example 1

[0126] Skin wrinkle test results (under the eyes)

[0127] VISIA was used to collect facial image information and analyze the photos. Feature counting means counting the feature parameters without calculating the size and intensity of the feature parameters. A smaller value indicates better skin; the score means evaluating the size, area, and intensity of the feature parameters. A smaller score indicates better skin.

[0128] Reference Figure 1 , compared with before using the sample, after using the sample, that is, the cream in Example 1 for 14 days, the feature count of skin wrinkles under the eyes was significantly reduced by 9.30%. After using the sample for 28 days, the feature count of skin wrinkles under the eyes was significantly reduced by 16.35%.

[0129] Test Example 2

[0130] Skin wrinkle test results (outer canthus)

[0131] Primos CR was used for facial image acquisition of skin wrinkles and photo analysis. A smaller analysis value indicates improvement in skin wrinkles.

[0132] Reference Figure 2 , compared with before using the sample, after using the sample, that is, the cream in Example 1 for 14 days, the number of skin wrinkles at the outer canthus was significantly reduced by 19.11%. After using the sample for 28 days, the number of skin wrinkles at the outer canthus was significantly reduced by 22.33%.

[0133] Test Example 3

[0134] Skin stratum corneum water content

[0135] Skin Moisture Content Tester CM825 is used to detect the moisture content of the skin stratum corneum. An increase in the measured value indicates an increase in the moisture content of the skin stratum corneum.

[0136] Reference Figure 3 , compared with before using the sample, after using the sample, that is, the cream of Example 1 for 14 days, the moisture content of the skin stratum corneum increased significantly by 14.86%. After using the sample for 28 days, the moisture content of the skin stratum corneum increased significantly by 27.24%.

[0137] Test Example 4

[0138] Skin Dermis Thickness

[0139] The collagen probe Dermlab Combo is used for facial image acquisition and photo analysis. An increase in the analysis value indicates an improvement in the thickness and density of the skin dermis.

[0140] Reference Figure 4 、 5 , compared with before using the sample, after using the sample, that is, the cream of Example 1 for 14 days, the skin dermis thickness increased significantly by 6.64%, and the skin dermis density increased significantly by 11.18%. After using the sample for 28 days, the skin dermis thickness increased significantly by 9.92%, and the skin dermis density increased significantly by 12.80%.

[0141] Compared with before using the product, after using the product for 14 days and 28 days, the count of skin wrinkles (under the eyes), the number of skin wrinkles (outer canthus), the water content of the skin stratum corneum, the thickness and density of the skin dermis all improved significantly. This shows that the product can fade fine lines, improve skin moisture, increase skin elasticity, improve facial roughness, and has good anti-aging effects.

[0142] After using the creams of different examples and comparative examples for 28 days, the change rates of D28 compared to D0 for skin wrinkles (under the eyes) count, skin wrinkles (outer canthus) number, skin stratum corneum moisture content, skin dermis thickness, and skin dermis density are shown in Table 5.

[0143] Table 5 Change rates of skin index D28 compared to D0 after 28 days using different examples and comparative examples

[0144]

[0145]

[0146] As can be seen from Table 5, the creams of Examples 1-5 are added with a composite fermentation product, bakuchiol, and rice fermentation liquid at the same time. The improvement effects in terms of the skin wrinkle (under the eyes) feature count, the number of skin wrinkles (outer canthus), the moisture content of the skin cutin layer, the thickness of the skin dermis layer, and the density of the skin dermis layer are better than those of the creams of Comparative Examples 1-6 that are added with only one or two of the composite fermentation product, bakuchiol, and rice fermentation liquid, and are even better than those of Comparative Example 7 that does not contain the composite fermentation product, bakuchiol, and rice fermentation liquid.

[0147] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art without departing from the scope of the present invention according to the disclosure of the present invention should be within the protection scope of the present invention.

Claims

1. A composition with anti-aging efficacy, characterized in that, It includes a composite fermentation product, bakuchiol, and rice fermentation broth, wherein the weight ratio of the composite fermentation product, bakuchiol, and rice fermentation broth is 1-3:1-2:1-3; The said composite fermentation product is prepared by the following method: (1)Activation of strains: Activate and culture the Candida bombicola strains. (2) Fermentation: After activation culture, Candida bombicola the strain is inoculated into the mixed oil of Alnus maximowiczii seed oil and the fermentation product of Candida apicola / glucose / methyl oleate for fermentation to obtain a preliminary fermentation product; (3) Purification: Centrifuge and filter the preliminary fermentation product obtained in step (2) to obtain a composite fermentation product containing various metabolites.

2. The composition with anti-aging effect according to claim 1, characterized in that In step (2), the weight ratio of the oil of Alnus rhombifolia Nutt. and the fermentation product of Candida apicola / glucose / methyl oleate is 1-2:1-2.

3. A composition with anti-aging effect according to claim 1, characterized in that, The weight ratio of the composite fermentation product, bakuchiol, and rice fermentation broth is 2:1:

2.

4. Use of the composition with anti-aging effect according to claim 1 or 2 or 3, characterized in that, The said composition with anti-aging efficacy is used for the preparation of cosmetics.

5. A cosmetic with anti-aging efficacy, characterized in that, It includes the composition with anti-aging efficacy described in claim 1 or 2 or 3.

6. A cream with anti-aging effect, characterized in that, It includes the composition with anti-aging efficacy described in claim 1 or 2 or 3.

7. The anti-aging cream according to claim 6, characterized in that, The said cream with anti-aging efficacy, based on the total amount of 100%, contains the following components in weight percentage: Composition with anti-aging efficacy 0.5-5% Humectant 15-30% Thickener 5-10% Antioxidant 1-2% Chelating agent: 0.1-0.5% Preservative: 0.1-0.5% Water the balance.

8. The anti-aging cream according to claim 6, characterized in that, The said cream with anti-aging efficacy, based on the total amount of 100%, contains the following components in weight percentage: Humectant: Squalane 3.5% Humectant: Isopropyl myristate 0.3% Thickener: Cyclopentasiloxane 7.0% Humectant: Glycerol 13.5% Antioxidant: Tocopherol 1.0% Humectant: 1,3-Butanediol 3.0% Thickener: Carbomer 0.4% Chelating agent: Disodium ethylenediaminetetraacetate 0.2% Preservative: Phenoxyethanol 0.1% Water the balance.

9. The preparation method of the cream with anti-aging effect according to claim 8, characterized in that, It includes the following steps: (1) Dissolve carbomer and place it in a constant temperature water bath. (2) Heat and stir squalane, isopropyl myristate, and cyclopentasiloxane until completely melted, and continue stirring after complete melting. (3) Heat and stir water, glycerol, tocopherol, 1,3-butanediol, and disodium ethylenediaminetetraacetate until completely melted, and continue stirring after complete melting. (4) Add the product of (3) to the product of (1), stir, then add the product of (2), and continue stirring. (5) Naturally cool the product of (4), add phenoxyethanol, and continue stirring and cooling to room temperature. (6) Add the composition with anti-aging efficacy to the product of (4), stir, and let it stand.

Images