Fermented water-in-oil double-layer composition, method for preparing the same, and use thereof

Abstract

The present application relates to a fermented water-oil double-layer composition and its preparation method and application, and belongs to the technical field of daily chemicals. The fermented water-oil double-layer composition is composed of an aqueous phase liquid and an oil phase liquid, wherein the weight ratio of the aqueous phase liquid to the oil phase liquid is 40-60:40-60; the aqueous phase liquid comprises yeast / rice fermentation product filtrate, yeast lysate extract, humectant and functional ingredients; the oil phase liquid comprises squalane, vegetable oil and fermented oil, and the fermented oil contains Candida oleaginosus / glucose / methyl oleate fermentation product, corn germ oil and glutathione. No additional water is added in the formula of the present application. Meanwhile, special oil fermentation strains are used to co-ferment with natural oil and active ingredients, so that the active ingredients are better dissolved and lysed in the oil phase, and natural fermented oil is used as the main component of the oil phase liquid. The present application has good moisturizing and penetrating properties, and can solve the greasy problem in the use feeling of pure oil phase essence oil, and the use feeling is refreshing and not greasy.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of daily chemicals, and particularly relates to a fermented water-oil double-layer composition and a preparation method and application thereof. BACKGROUND

[0002] Essence oil is a kind of cosmetic with oil phase composition as the main component, which can effectively supplement skin oil, relieve dry skin, and has no luster. It can also soften the skin, improve rough skin, make the skin more and more soft, delay skin aging, repair damaged cells, and accelerate the circulation of skin blood.

[0003] The conventional essence oil is high in oil content and is greasy to use, which is not suitable for oily skin. The water-oil double-layer essence oil is a kind of essence oil popular on the market at present, which is obtained by adding water to the conventional essence oil with oil phase composition as the main component. The water-oil double-layer essence oil can not only effectively supplement skin oil, but also can supplement water, and is suitable for people with damaged skin protection barrier to use for adjusting water-oil balance. However, the water phase liquid in the ordinary water-oil double-layer essence oil only uses pure water, which makes it difficult for water to enter the inside of the skin and achieve the effect of water supplement.

[0004] Chinese patent CN113693977A discloses a rose essence oil and a preparation method thereof. The rose essence oil is composed of a water phase liquid and an oil phase liquid. The components of the water phase liquid are deionized water, sodium chloride, xanthan gum, attar of rose, dipropylene glycol, glycerol, p-hydroxyacetophenone, and 1,2-hexanediol. The components of the oil phase liquid are ethylhexyl palmitate, tocopherol, squalane, isododecane, and rose oil. The patent changes the surface tension by affecting the intermolecular and intramolecular forces between water molecules and oil molecules under the joint action of sodium chloride, glycerol, and dipropylene glycol, and finally obtains the rose essence oil. This method can stably mix the water phase liquid and the oil phase liquid. However, the patent still has the problem that water is not easily absorbed by the skin. SUMMARY

[0005] The present application provides a fermented water-oil double-layer composition and a preparation method and application thereof. By selecting the main components of the water phase liquid and the oil phase liquid, good skin permeability is achieved, and the skin deep layer is quickly penetrated to achieve the effect of moisturizing and water supplement.

[0006] The object of the present application can be achieved by the following technical solutions:

[0007] The present application provides a fermented water-oil double-layer composition, which is composed of a water phase liquid and an oil phase liquid, and the weight ratio of the water phase liquid to the oil phase liquid is 40-60:40-60.

[0008] The water phase liquid comprises a yeast / rice fermentation product filtrate, a yeast lysate extract, a humectant, and an efficacy component.

[0009] The oil phase liquid comprises squalane, vegetable oil and fermentation oil,

[0010] The fermentation oil is obtained by fermenting corn germ oil, glucose, rapeseed oil and glutathione together by Candida bombicola, and contains Candida bombicola / glucose / rapeseed oil acid methyl ester fermentation product, corn germ oil and glutathione. The three components have a synergistic effect.

[0011] In the present application, the corn germ oil, glucose, rapeseed oil and glutathione are fermented together by Candida bombicola, and the biological stability and activity of glutathione can be improved through the oil phase co-fermentation.

[0012] In one embodiment of the present application, the preparation method of the fermentation oil comprises the following steps:

[0013] The corn germ oil, rapeseed oil, glucose and glutathione are added into a fermentation tank to obtain a mixed oil substrate;

[0014] The Candida bombicola is inoculated into the mixed oil substrate, and the fermentation liquid is obtained after fermentation;

[0015] The upper oil of the fermentation liquid is collected by centrifugation;

[0016] The fermentation oil is obtained by filtration and purification.

[0017] In one embodiment of the present application, the weight ratio of the corn germ oil, Candida bombicola / glucose / rapeseed oil acid methyl ester fermentation product and glutathione in the fermentation oil is 10:3-4:0.1-1.

[0018] In one embodiment of the present application, the weight ratio of the yeast / rice fermentation product filtrate, yeast lysate extract, humectant and functional ingredient in the water phase liquid is 8-20:5-15:10-20:1-5.

[0019] In one embodiment of the present application, the humectant is selected from one or a combination of several of glycerol, butylene glycol, pentylene glycol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, trehalose and acetylated sodium hyaluronate.

[0020] In one embodiment of the present application, the humectant is a combination of glycerol, butylene glycol, pentylene glycol, sodium hyaluronate cross-linked polymer, sodium hyaluronate and acetylated sodium hyaluronate.

[0021] In one embodiment of the present application, the efficacy ingredient is selected from the group consisting of dipotassium glycyrrhizate, ethylhexylglycerin, xanthan gum, hydroxymethylcellulose, arginine, trehalose, hexylene glycol, sodium lactate, or a combination thereof.

[0022] In one embodiment of the present application, the efficacy ingredient further comprises a cosmetic acceptable preservative, which can be selected from phenoxyethanol.

[0023] In one embodiment of the present application, the efficacy ingredient is selected from the group consisting of dipotassium glycyrrhizate, ethylhexylglycerin, xanthan gum, hydroxymethylcellulose, arginine, trehalose, hexylene glycol, sodium lactate, or a combination thereof.

[0024] In one embodiment of the present application, the weight ratio of squalane, plant oil and fermented oil in the oil phase is 10-30:30-40:10-20.

[0025] In one embodiment of the present application, the plant oil is selected from the group consisting of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil, Olea europaea fruit oil, or a combination thereof.

[0026] In one embodiment of the present application, the plant oil is selected from the group consisting of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil, Olea europaea fruit oil, or a combination thereof.

[0027] Different plant oils have different fatty acid ratios, resulting in different skin feel experiences, and contain different types of active ingredients. By studying and optimizing the reasonable ratio of different plant oils, better moisturizing efficacy can be achieved. In the present application, the combination of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil and Olea europaea fruit oil will have better moisturizing function.

[0028] In one embodiment of the present application, the weight ratio of the aqueous phase and the oil phase in the fermented water-oil double-layer composition is 44-52:48-56; for example, it can be 44:56 or 51.8:48.2. Since the fermented oil has amphiphilic properties after fermentation, compared with pure plant oil, it can form specific micelles with the aqueous phase. The mixture of the aqueous phase and the oil phase can help reduce the greasy feeling during use, and the oil phase can easily penetrate into the inner layer of the skin.

[0029] The present application further provides a preparation method of the fermentation water-oil double-layer composition, comprising the following steps:

[0030] Preparation of the water phase liquid: the yeast / rice fermentation product filtrate, the yeast lysate extract, the humectant and the functional ingredient are mixed and stirred uniformly to obtain the water phase liquid;

[0031] Preparation of the oil phase liquid: the squalane, the vegetable oil and the fermentation oil component are mixed to obtain the oil phase liquid;

[0032] Mixing the water phase liquid and the oil phase liquid according to the designed proportion to obtain the fermentation water-oil double-layer composition.

[0033] In the preparation method of the fermentation water-oil double-layer composition, the method for mixing the components constituting the water phase liquid can adopt a conventional stirring and dissolving method.

[0034] In the preparation method of the fermentation water-oil double-layer composition, the method for mixing the components constituting the oil phase liquid can adopt a conventional stirring and dissolving method.

[0035] The present application further provides the application of the fermentation water-oil double-layer composition, which is used as cosmetics, such as essence oil, emulsion and the like.

[0036] In the present application, the roles of some main components are introduced as follows:

[0037] In the present application, the yeast / rice fermentation product filtrate is naturally fermented from rice by natural yeast, and is rich in various active ingredients required by the skin, such as oligosaccharides, organic acids, polypeptides, amino acids and the like. It can be simply and quickly absorbed and utilized by the metabolic activity of common probiotics in the skin (such as: lactobacillus pentosus, micrococcus kielmayeri and the like), but not absorbed and utilized by common pathogenic bacteria in the skin (such as: propionibacterium acnes, staphylococcus aureus), thereby helping the healthy growth of the probiotic group in the skin, and competitively inhibiting the growth and reproduction of pathogenic bacteria, establishing a good probiotic ecological environment beneficial to the skin; at the same time, it has the effects of rapidly activating skin cells, inhibiting skin inflammation and strengthening the skin keratin protective barrier.

[0038] In the present application, the yeast lysate extract can play multiple effects, such as being capable of improving the five major problems of the skin: dullness, pigmentation, roughness and dryness, uneven water and oil, and relaxation and fine lines.

[0039] The specific effects of the yeast lysate extract include:

[0040] 1. Fully supply cell nutrients: Yeast lysate extract is rich in various nutrients, such as a high proportion of amino acids, nucleosides, nucleotides, peptides, active enzymes, amino acid precursors, sterols, trace elements, vitamin B, etc. in the lysate, which are basically active substances that can be directly utilized, providing basic nutrition for skin cells and helping skin cells to be healthy and vigorous.

[0041] 2. Lighten melanin and whiten skin: Scientists have found that adding live yeast to melanocytes can inhibit melanin formation by more than 60% compared to melanocytes without live yeast. It is confirmed that live yeast has excellent whitening effect.

[0042] 3. Enhance cell respiration: The life and division times of skin cells are limited, and when skin cells approach the limit of division times, they will gradually become a state of "having gas but no power". Yeast lysate extract can enhance cell respiration, and the higher the efficiency of cell oxygen consumption, the less the formation of destructive reactive oxygen species (ROS).

[0043] 4. Soothe the skin and promote repair ability: Yeast lysate extract can significantly relieve the discomfort such as redness and pain caused by skin inflammatory reaction, especially the red hot feeling of skin after mild sunburn. At the same time, it has the ability to accelerate the healing and repair of epidermal minor wounds, helping the skin to be more comfortable and stable.

[0044] 5. Antioxidant / collagen synthesis promotion: Yeast lysate contains various coenzyme components, which have the effect of reducing free radical oxidative damage, and yeast lysate can also increase the formation and expression of skin fibroblasts, promote the synthesis of type I collagen, and prevent skin relaxation and fine lines.

[0045] In the present application, squalane is similar to squalene, an important component of human sebum. Squalane is the hydrogenation product of squalene, has the effects of skin-friendly, moisturizing, and antioxidant, and is similar to sebum components, more easily absorbed into the skin, and forms a protective film on the skin surface, playing a barrier role.

[0046] In the present application, the fermentation product of Candida bombicola / glucose / methyl oleate plays the role of a plant oil fermentation product with a hydrophilic-lipophilic balance. Its special structure causes it to have certain emulsifying ability, and it has lower surface tension compared to unfermented plant oil. It can break the interface existing in the interstitial space between keratinocytes (proteins) and quickly penetrate into the deep layer of the skin. It is more hydrophilic and tightly combined with the water in the stratum corneum to prevent water loss. Its main functions are emollient, antibacterial, and oil control.

[0047] In the present application, corn germ oil is extracted from corn germ. Corn germ oil is rich in vitamin E and unsaturated fatty acids such as linoleic acid and oleic acid, which are essential for the human body, can protect sebum and cell membrane proteins and moisture in the skin. It can also promote cell regeneration and vitality, delay the aging process of cells, and have a nourishing effect on collagen fibers and elastic fibers in the skin. The main role in cosmetics and skin care products is skin conditioner, emollient, and is relatively safe.

[0048] In the present application, glutathione is an important antioxidant in the body, which can remove free radicals in the body. Because it is easily oxidized by certain substances, it can protect the sulfhydryl groups in many proteins and enzyme molecules in the body from being oxidized by harmful substances, thereby ensuring the normal physiological function of protein and enzyme molecules. Glutathione has good whitening effect in the field of cosmetics, and can also lighten melanin deposition. Combined with its inhibition of elastase and excellent water retention capacity, it is used for skin conditioning.

[0049] In the present application, fermentation is used to help the active ingredient glutathione dissolve into the fermented oil. The fermentation process can help glutathione better dissolve in the oil phase, protect it from oxidation by free radicals, and exert its activity.

[0050] The fermented oil described in the present application contains Candida bombicola / glucose / rapeseed fatty acid methyl ester fermentation product, corn germ oil, and glutathione. After separation and purification, the free fatty acids produced during the fermentation process are removed, the acid value is reduced, and the antioxidant capacity is further improved.

[0051] Compared with the prior art, the present application has the following advantages:

[0052] In the present application, yeast / rice fermentation product filtrate is used instead of water as the main component of the aqueous phase liquid, and is compounded with other functional ingredients. No additional water is added to the formula. Fermented oil is also used as the main component of the oil phase liquid. The aqueous phase liquid and the oil phase liquid are set at a special ratio, which has good moisturizing and penetrating properties, can solve the problem of greasiness in the use of pure oil phase essence oil, and has a refreshing and non-greasy use experience.

[0053] In the present application, in addition to conventional plant oils, fermented oil is particularly selected as the oil phase. Fermented oil includes corn germ oil, Candida bombicola / glucose / rapeseed fatty acid methyl ester fermentation product, and glutathione. Fermented oil, special lipophilic microorganisms, and the process of fermentation can help plant essence, glutathione has anti-inflammatory effect, and enter the oil phase to carry functional ingredients to enable sensitive skin anti-inflammatory effect.

[0054] In the present application, both fermented oil and yeast / rice fermentation product filtrate have good penetrating properties, can quickly penetrate into the deep layer of the skin, and have the effect of moisturizing and hydrating.

[0055] In the present application, the formula design meets the definition of natural ingredient technology, and the natural ingredient index can reach more than 99% according to ISO16128. DETAILED DESCRIPTION

[0056] In the following examples, the preparation method of the fermented oil includes the following steps:

[0057] The corn germ oil, rapeseed oil, glucose and glutathione are added to the fermenter, mixed to obtain an oil mixture substrate, and high-temperature sterilization is performed at 100-120°C for 10-20 minutes;

[0058] The Candida bombicola is inoculated into the oil mixture substrate after high-temperature sterilization, the temperature of the fermentation substrate is controlled at 20-40°C, and stirring is performed, and the fermentation liquid is obtained after fermentation for 24-48 hours;

[0059] The upper oil of the fermentation liquid is collected by centrifugation;

[0060] The fermented oil is obtained by filtration and purification.

[0061] The active ingredient glutathione is dissolved into the fermented oil by fermentation, and after separation and purification, the free fatty acids produced in the fermentation process are removed, the acid value is reduced, and the antioxidant capacity is improved.

[0062] The present application will be described in detail below with reference to specific examples.

[0063] Example 1

[0064] A specific fermented water-oil double-layer composition is provided, which can be used as a specific essence, and the formula is shown in Table 1:

[0065] Table 1 Formula of fermented water-oil double-layer composition of Example 1

[0066]

[0067]

[0068]

[0069] No water is added during the preparation of the formula.

[0070] The preparation process of the fermented water-oil double-layer composition is as follows:

[0071] 1) Ensure that the production equipment is dry, and sequentially add the A-phase raw materials to the main tank, stir and mix at room temperature at 100 rpm for 30 minutes, and dissolve uniformly;

[0072] 2) Then add the B-phase raw materials to the oil pot, stir uniformly, 30 revolutions per minute, and maintain for 30 minutes;

[0073] 3) Mix the aqueous phase liquid A and the oil phase liquid B in the designed ratio to obtain the fermentation water-oil double-layer composition.

[0074] The following compositions in the form of combinations are provided as comparative examples, Comparative Examples 1 and 2, and the formulations are shown in Table 2 (values are mass percentages):

[0075] Table 2 Formulations of compositions of Comparative Examples 1 and 2

[0076]

[0077]

[0078]

[0079] Comparative Examples 3-8: Compared with Example 1, the components, amounts, and preparation methods of the fermentation oil are the same, and the difference between Comparative Examples 3-8 and Example 1 is that the fermentation oil in Example 1 is replaced by one or two of the following specific components: corn germ oil, Candida oleaginosus / glucose / canola oil acid methyl ester fermentation product, and glutathione. The specific differences between Example 1 and Comparative Examples 3-8 are shown in Table 3 below.

[0080] Table 3 Differences in formulations between Example 1 and Comparative Examples 3-8

[0081]

[0082] Comparative Examples 9-10: Compared with Example 1, the components, amounts, and preparation methods of the plant oil are the same, and the difference between Comparative Examples 9-10 and Example 1 is that the plant oil of Example 1, which is a combination of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil, and Olea europaea fruit oil, is replaced by using only 2-3 of the above-mentioned oils. The specific differences between Example 1 and Comparative Examples 9-10 are shown in Table 4 below.

[0083] Table 4 Differences in formulations between Example 1 and Comparative Examples 9-10

[0084]

[0085] The preparation process of the fermentation water-oil double-layer composition is as follows:

[0086] 1) Ensure that the production equipment is dry, and then sequentially add the A-phase raw materials to the main tank, stir and mix at room temperature at 100 rpm for 30 minutes, and dissolve uniformly;

[0087] 2) Then add the B-phase raw materials to the oil pot, stir and mix uniformly, at 30 rpm for 30 minutes;

[0088] 3) Mix the A-phase and B-phase liquids in the designed ratio to obtain the fermented water-oil double-layer composition.

[0089] Example 2

[0090] A specific fermented water-oil double-layer composition is provided, with a water-oil ratio of 51.8:48.2, which can be used as a serum. The formula is shown in Table 5:

[0091] Table 5 Formula of Example 2

[0092]

[0093]

[0094]

[0095] No water is added during the preparation of the formula.

[0096] The preparation process of the fermented water-oil double-layer composition is as follows:

[0097] 1) Ensure that the production equipment is dry, and then sequentially add the A-phase raw materials to the main tank, stir and mix at room temperature at 100 rpm for 30 minutes, and dissolve uniformly;

[0098] 2) Then add the B-phase raw materials to the oil pot, stir and mix uniformly, at 30 rpm for 30 minutes;

[0099] 3) Mix the A-phase and B-phase liquids in the designed ratio to obtain the fermented water-oil double-layer composition.

[0100] To verify that the fermented water-oil double-layer composition of the present application has soothing effect and is suitable for sensitive skin, the following effect verification test is conducted.

[0101] Effect verification test examples

[0102] 1. Safety test

[0103] Safety test is conducted on Examples 1-2;

[0104] Test method: A third-party testing institution is used to test safety. An area of not more than 50 mm 2Suitable patch device with a depth of about 1mm, about 0.02-0.025ml (g) of the test substance (product of Example 1-2) was added to the patch device by closed patch test method. The patch device was attached to the flexor side of the forearm of the subject (130 people), and the test substance was removed after 24h. The skin reaction was observed at 0.5h, 24h, 48h after removal, respectively, and the results were recorded according to the skin reaction grading standard in the Cosmetic Safety Technology Regulation 2015.

[0105] Note: Skin reaction grading standard;

[0106] 0: Negative reaction;

[0107] 1: Suspected reaction; only weak erythema;

[0108] 2: Weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0109] 3: Strong positive reaction (vesicle reaction); erythema, infiltration, edema, papules, and vesicles; the reaction may extend beyond the test area;

[0110] 4: Very strong reaction (fusion vesicle reaction); obvious erythema, severe infiltration, edema, fusion vesicles, and the reaction extends beyond the test area.

[0111] The results show that the products of Examples and Comparative Examples have a score of 0 at 30min, 24h, and 48h, and no skin adverse reactions occur, indicating that the fermented water-oil double-layer composition product involved in the present application is safe and non-irritating to the skin.

[0112] 2. Moisturizing and soothing effect evaluation

[0113] Test sample: samples of Example 1 and Comparative Examples 1-10;

[0114] Test method: The test sample was applied at a dosage of (2±0.1) mg / cm 2 The sample was evenly applied to the inner side of the left or right forearm using a latex glove, and a single use lasted for 6 hours. The control group was a blank control without using any cosmetics.

[0115] The results were tested at the following four time periods: before using the sample (D0), 1h after using the sample, 3h after using the sample, and 6h after using the sample.

[0116] Detection items:

[0117] Skin moisture meter Used for detecting the water content of the stratum corneum.

[0118] Test results:

[0119] As shown in Table 6-7, the efficacy test results of Example 1, the moisture content of the stratum corneum of the skin in the sample area was significantly increased after 1h, 3h, 6h of using the sample, and increased by 21.82%, 26.48%, and 27.02% respectively.

[0120] Table 6 Moisture content of stratum corneum

[0121]

[0122]

[0123] Table 7 Change rate of moisture content of stratum corneum

[0124] Time 1 h after use 3 h after use 6 h after use Example 1 21.82％ 26.48％ 27.02％ Comparative Example 1 10.86％ 16.69％ 15.90％ Comparative Example 2 5.69％ 7.28％ 7.72％ Comparative Example 3 10.29％ 10.86％ 11.43％ Comparative Example 4 10.32％ 11.10％ 11.54％ Comparative Example 5 10.30％ 11.53％ 11.60％ Comparative Example 6 8.42％ 9.57％ 11.04％ Comparative Example 7 8.59％ 9.91％ 11.09％ Comparative Example 8 8.43％ 9.76％ 11.03％ Comparative Example 9 18.96％ 19.80％ 21.21％ Comparative Example 10 17.55％ 19.37％ 20.66％

[0125] As can be seen from Table 6 and Table 7, the fermentation oil containing Candida oleaginosus / glucose / methyl oleate fermentation product, corn germ oil, and glutathione in the examples has better moisturizing and soothing effect than the comparative examples 3-8 containing only two or one of the three ingredients, proving that Candida oleaginosus / glucose / methyl oleate fermentation product, corn germ oil, and glutathione have a synergistic effect. At the same time, the simultaneous use of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil, and Olea europaea fruit oil in the examples has better moisturizing and soothing effect than the comparative examples 9-10 using only 2-3 of the four ingredients, proving that the simultaneous use of Camellia oleifera seed oil, Helianthus annuus seed oil, Camellia japonica seed oil, and Olea europaea fruit oil has a synergistic effect.

[0126] 3. Lactic acid stinging test

[0127] Test sample: Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 7

[0128] Test method: 10% lactic acid or distilled water 50 μL was dropped on filter paper with a diameter of 8 mm, and placed on the bilateral nasolabial folds of the subjects (50 people, 10 people in each group, and the products of the examples and the comparative examples were used in each group, respectively). The subjects evaluated the discomfort of itching, stinging, and burning sensation at the test site at 30 s, 2.5 min, and 5 min, respectively. The scoring was performed according to a 4-point scale (0 point for no feeling, 1 point for mild, 2 points for moderate, and 3 points for severe). The sum of the stinging scores at 2.5 min and 5 min was ≥3 points, and the lactic acid stinging test was positive.

[0129] After 4 weeks of continuous use, the lactic acid stinging test was also performed according to the above method.

[0130] As shown in the results of Table 8 (average value of each group of subjects), the serum obtained in Example 1 of the present application can effectively repair the skin barrier and reduce the lactic acid stinging value, and the repair effect is ranked as Example 1 > Comparative Example 1 > Comparative Example 3 > Comparative Example 7 > Comparative Example 2, proving that the composition with skin barrier repair function has better skin barrier repair effect through the synergistic effect between components than the combination of only one or two components.

[0131] The serum containing the fermented water-oil double-layer composition is suitable for sensitive skin.

[0132] Table 8 Sensory score at each time point of Example 1

[0133] Lactic acid stinging value W0 (2.5 min + 5 min) W4 (2.5 min + 5 min) Example 1 3.60 1.71 Comparative Example 1 3.64 1.91 Comparative Example 2 3.61 3.20 Comparative Example 3 3.63 2.03 Comparative Example 7 3.60 2.25

[0134] The above effect test proves that the fermented water-oil double-layer composition in the present example can change the surface tension of water and oil through the process of microbial fermentation, making it more skin-friendly, and achieving good skin permeability, so as to quickly penetrate into the deep layer of the skin and achieve the effect of moisturizing and hydrating.

[0135] The above description of the examples is for the convenience of the ordinary skilled person in the art to understand and use the application. Those skilled in the art can obviously make various modifications to these examples, and apply the general principles described herein to other examples without the need for creative labor. Therefore, the present application is not limited to the above examples, and the improvements and modifications made by those skilled in the art without departing from the scope of the present application should be within the scope of protection of the present application.

Claims

1. A fermented water-oil double-layer composition, characterized in that: It is composed of an aqueous phase liquid and an oily phase liquid, wherein the weight ratio of the aqueous phase liquid to the oily phase liquid is 40-60:40-60; The aqueous phase liquid includes yeast / rice fermentation product filtrate, yeast lysate extract, moisturizer and functional ingredients; The oil phase liquid includes squalane, vegetable oil and fermented oil, wherein the fermented oil is obtained by fermenting corn germ oil, glucose, rapeseed oil and glutathione using Candida mellifera. The fermented oil contains a fermentation product of Candida mellifera / glucose / rapeseed oleic acid methyl ester, corn germ oil and glutathione, and the weight ratio of the corn germ oil, the fermentation product of Candida mellifera / glucose / rapeseed oleic acid methyl ester and the glutathione in the fermented oil is 10:3-4:0.1-1. The vegetable oil is selected from the group consisting of camellia seed oil, sunflower seed oil, camellia seed oil and olive oil; The moisturizing agent is selected from one or a combination of glycerin, butylene glycol, pentylene glycol, disodium EDTA, sodium hyaluronate cross-linked polymer, sodium hyaluronate, and acetylated sodium hyaluronate; The effective ingredient is selected from one or a combination of dipotassium glycyrrhizate, ethylhexylglycerin, xanthan gum, hydroxymethyl cellulose, phenoxyethanol, arginine, trehalose, hexylene glycol, and sodium lactate.

2. A fermented water-oil double-layer composition according to claim 1, characterized in that: In the aqueous phase, the weight ratio of the yeast / rice fermentation product filtrate, the yeast lysate extract, the moisturizer and the functional component is 8-20:5-15:10-20:1-5.

3. A fermented water-oil double-layer composition according to claim 1, characterized in that: In the oil phase liquid, the weight ratio of squalane, vegetable oil and fermented oil is 10-30:30-40:10-20.

4. A fermented water-oil double-layer composition according to claim 1, characterized in that: In the fermented water-oil double-layer composition, the weight ratio of the water phase liquid to the oil phase liquid is 44-52:48-56.

5. The method for preparing the fermented water-oil double-layer composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: Preparing an aqueous phase liquid: mixing components constituting the aqueous phase liquid to obtain an aqueous phase liquid; Preparing the oil phase liquid: mixing the components constituting the oil phase liquid to obtain the oil phase liquid; The aqueous phase liquid and the oil phase liquid are mixed to obtain a fermented water-oil double-layer composition.

6. Use of the fermented water-oil bilayer composition according to any one of claims 1 to 4 in the preparation of cosmetics.

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