A blood analyzer and hemoglobin detection method

Abstract

A blood analyzer and a hemoglobin detection method, the method comprising: a control device controls a sampling device (11) to obtain a sample to be tested (201); a control device controls a sample preparation device (12) to mix a portion of the sample to be tested with a first reagent to prepare a first sample liquid to be tested, and a control device controls a portion of the sample to be tested with a second reagent to prepare a second sample liquid to be tested (202); a control device controls a colorimetric detection device (13) to perform a hemoglobin detection on the first sample liquid to be tested using a colorimetric method to obtain a first measurement value (203); a control device controls an optical detection device (14) to perform a hemoglobin detection on the second sample liquid to be tested using a light scattering method to obtain a second measurement value (204); and a control device determines whether the sample to be tested is a sample with a falsely high hemoglobin level (205); if the sample to be tested is a sample with a falsely high hemoglobin level, the control device outputs a second measurement value (206); if the sample to be tested is a sample with a falsely high hemoglobin level, the control device outputs a first measurement value (207).

Description

Technical Field

[0001] The present application relates to the field of medical testing, in particular to the field of blood protein testing, and is related to but not limited to a blood analyzer and a hemoglobin testing method. Background Art

[0002] Human blood contains various cells, including red blood cells, white blood cells, and platelets. Red blood cells contain hemoglobin. Hemoglobin (HGB, or hemoglobin, Hb) is a specialized protein that transports oxygen within red blood cells and gives blood its red color. It is composed of globin and heme, with the globin portion being a tetramer consisting of two pairs of different globin chains. Hemoglobin has similar utility to red blood cells, and its elevation or decline is of great significance in clinical medicine. Hemoglobin levels are particularly useful for measuring the severity of anemia.

[0003] As is known to all, the measurement method of hemoglobin is generally colorimetric. The measurement principle of colorimetric method is that the different solution concentrations of the sample to be tested correspond to different solution colors. Generally, the higher the solution concentration, that is, the higher the hemoglobin content, the darker the color of the solution. Conversely, the lower the solution concentration, that is, the lower the hemoglobin content, the lighter the solution color. Among them, the depth of the solution color of the sample to be tested directly affects the degree of absorption of light by the sample to be tested. That is, the darker the solution color of the sample to be tested, the more light it absorbs and the less light it transmits. Conversely, the lighter the solution color, the less light it absorbs and the more light it transmits. Therefore, by measuring the intensity of the light passing through the colorimetric cell, the solution concentration of the sample to be tested can be measured.

[0004] However, when the sample to be tested is abnormal, for example, if the sample to be tested is a chylomicronized blood sample, a high value of white blood cells (WBC) or bilirubin, etc., it will cause a falsely high HGB. For example, due to the presence of chylomicrons, part of the light is scattered, and the transmitted light is reduced, resulting in a falsely high hemoglobin concentration test value obtained by the colorimetric method. At present, the clinic usually uses manual methods to determine whether the sample to be tested is chylomicronized blood. When the sample to be tested is manually determined to be chylomicronized blood, a plasma exchange experiment is used to remove the chyle, and then the sample to be tested after the chyle is removed is tested to obtain an accurate hemoglobin concentration test value. This method is time-consuming and labor-intensive, and requires high skills of the operator.

[0005] Due to the existence of the above-mentioned abnormalities, the current hemoglobin testing instruments cannot distinguish whether the tested sample has abnormalities, resulting in low accuracy of the hemoglobin test results and low intelligence of the hemoglobin testing instruments. Summary of the Invention

[0006] In view of this, the embodiments of the present application hope to provide a blood analyzer and a hemoglobin detection method, which solves the current problem of low accuracy in hemoglobin testing, realizes automatic judgment of whether there is an abnormality in the sample to be tested, and determines the subsequent automatic processing method based on the judgment result, thereby ensuring the accuracy of the hemoglobin concentration test results.

[0007] A first aspect of the present application provides a blood analyzer, comprising:

[0008] A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested;

[0009] The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested;

[0010] a colorimetric detection device for performing a hemoglobin test on a first sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first sample solution is prepared by combining a portion of the sample solution and a first reagent;

[0011] an optical detection device for performing a hemoglobin detection on a second sample solution prepared by the sample preparation device using a light scattering method to obtain a second measurement value, wherein the second sample solution is prepared by a portion of the sample solution and a second reagent;

[0012] a processor electrically connected to the optical detection device and the colorimetric detection device, and configured to:

[0013] Obtaining the first measurement value and the second measurement value from the colorimetric detection device and the optical detection device;

[0014] Determining whether the sample to be tested is a sample with falsely high hemoglobin;

[0015] If the sample to be tested is the sample with falsely high hemoglobin, outputting the second measurement value;

[0016] If the sample to be tested is not the sample with falsely high hemoglobin, the first measurement value is output.

[0017] A second aspect of the present application provides a blood analyzer, comprising:

[0018] A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested;

[0019] The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested;

[0020] A colorimetric detection device, used for detecting hemoglobin in the sample to be tested using a colorimetric method;

[0021] An optical detection device for detecting hemoglobin in the sample to be tested using a light scattering method;

[0022] a processor electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and configured to:

[0023] Determining whether the sample to be tested is the sample with falsely high hemoglobin;

[0024] If the sample to be tested is not the sample with falsely high hemoglobin, controlling the sample preparation device to prepare a portion of the sample to be tested with a first reagent to obtain a first sample solution to be tested, and controlling the colorimetric detection device to perform a hemoglobin test on the first sample solution to obtain a first measurement value;

[0025] If the sample to be tested is the sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample solution to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample solution to obtain a second measurement value.

[0026] A third aspect of the present application provides a blood analyzer, comprising:

[0027] A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested;

[0028] The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested;

[0029] a colorimetric detection device for performing a hemoglobin test on a first sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first sample solution is prepared by combining a portion of the sample solution and a first reagent;

[0030] An optical detection device for detecting hemoglobin in the sample to be tested using a light scattering method;

[0031] a processor electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and configured to:

[0032] Determining whether the sample to be tested is the sample with falsely high hemoglobin;

[0033] If the sample to be tested is not the sample with falsely high hemoglobin, outputting the first measurement value;

[0034] If the sample to be tested is the sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample liquid to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample liquid to be tested to obtain a second measurement value, and the second measurement value is output.

[0035] A fourth aspect of the present application provides a hemoglobin detection method, the method comprising:

[0036] Obtaining samples to be tested;

[0037] Obtaining a portion of the sample to be tested to obtain at least a first sample and a second sample, and mixing the first sample with a first reagent to prepare a first sample solution to be tested, and mixing the second sample with a second reagent to prepare a second sample solution to be tested;

[0038] Performing a hemoglobin test on the first test sample liquid using a colorimetric method to obtain a first measurement value;

[0039] performing a hemoglobin test on the second test sample liquid using a light scattering method to obtain a second measurement value;

[0040] Determining whether the sample to be tested is the sample with falsely high hemoglobin;

[0041] If the sample to be tested is the sample with falsely high hemoglobin, outputting the second measurement value;

[0042] If the sample to be tested is not the sample with falsely high hemoglobin, the first measurement value is output.

[0043] A fifth aspect of the present application provides a hemoglobin detection method, the method comprising:

[0044] Obtaining samples to be tested;

[0045] Determining whether the sample to be tested is the sample with falsely high hemoglobin;

[0046] If the sample to be tested is not the sample with falsely high hemoglobin, a portion of the sample to be tested is obtained from the sample to be tested to obtain a first sample, and the first sample is mixed with a first reagent to prepare a first sample solution to be tested;

[0047] Performing a hemoglobin test on the first test sample liquid using a colorimetric method to obtain a first measurement value;

[0048] If the sample to be tested is the sample with falsely high hemoglobin, a portion of the sample to be tested is obtained from the sample to be tested to obtain a second sample, and the second sample is mixed with a second reagent to prepare a second sample solution to be tested;

[0049] The hemoglobin of the second sample liquid to be tested is detected by light scattering method to obtain a second measurement value.

[0050] A sixth aspect of the present application provides a hemoglobin detection method, the method comprising:

[0051] Obtaining samples to be tested;

[0052] Taking a portion of the sample to be tested to obtain a first sample, and mixing the first sample with a first reagent to prepare a first sample solution to be tested;

[0053] Performing a hemoglobin test on the first test sample liquid using a colorimetric method to obtain a first measurement value;

[0054] Determining whether the sample to be tested is the sample with falsely high hemoglobin;

[0055] If the sample to be tested is not the sample with falsely high hemoglobin, outputting the first measurement value;

[0056] If the sample to be tested is the sample with falsely high hemoglobin, a portion of the sample to be tested is obtained to obtain a second sample, and the second sample is mixed with a second reagent to prepare a second sample solution to be tested;

[0057] The hemoglobin of the second sample liquid to be tested is detected by light scattering method to obtain a second measurement value, and the second measurement value is output.

[0058] The blood analyzer and hemoglobin detection method provided in the embodiments of the present application automatically determine whether the sample to be tested is a sample with falsely high hemoglobin levels, and when the sample to be tested is a sample with falsely high hemoglobin levels, output a second measurement value obtained by performing hemoglobin detection on a second sample solution prepared from a portion of the sample to be tested using a light scattering method, or when the sample to be tested is not a sample with falsely high hemoglobin levels, output a first measurement value obtained by performing hemoglobin detection on a first sample solution prepared from a portion of the sample to be tested using a colorimetric method. This solves the current problem of low efficiency in hemoglobin testing due to the need for manual determination of whether the sample to be tested is a sample with falsely high hemoglobin levels, and achieves automated determination of whether the sample to be tested is a sample with falsely high hemoglobin levels, determines a subsequent automatic processing method based on the determination result, and outputs a hemoglobin concentration test result obtained by the corresponding automatic processing method, thereby ensuring the accuracy of the hemoglobin concentration test result. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] Figure 1 A schematic diagram of the structure of a blood analyzer provided in an embodiment of the present application;

[0060] Figure 2 A schematic structural diagram of another blood analyzer provided in an embodiment of the present application;

[0061] Figure 3 A schematic diagram of a blood analysis method provided in an embodiment of the present application;

[0062] Figure 4 A schematic diagram of an application scenario for determining whether a sample to be tested is a chylous blood sample based on a scatter plot provided in an embodiment of the present application;

[0063] Figure 5 A schematic diagram of another application scenario of determining that a sample to be tested is not a chylous blood sample based on a scatter plot provided in an embodiment of the present application;

[0064] Figure 6 A schematic flow chart of another blood analysis method according to an embodiment of the present application;

[0065] Figure 7 This is a flow chart of another blood analysis method according to an embodiment of the present application. DETAILED DESCRIPTION

[0066] To make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the specific technical solutions of the invention will be further described in detail below with reference to the accompanying drawings. The following embodiments are used to illustrate the present application but are not intended to limit the scope of the present application.

[0067] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. The terms used herein are for the purpose of describing the embodiments of this application only and are not intended to limit this application.

[0068] In the following description, reference is made to “some embodiments”, which describes a subset of all possible embodiments, but it will be understood that “some embodiments” may be the same subset or different subsets of all possible embodiments and may be combined with each other without conflict.

[0069] It should be pointed out that the terms "first\second\third" involved in the embodiments of the present application are only used to distinguish similar objects and do not represent a specific ordering of the objects.

[0070] The present application embodiment provides a blood analyzer, referring to Figure 1 As shown, the blood analyzer includes: a sampling device 11, a sample preparation device 12, a colorimetric detection device 13, an optical detection device 14 and a processor 15; wherein:

[0071] The sampling device 11 includes a pipette and a driving unit, which is used to drive the pipette to absorb the sample to be tested; wherein the pipette can have a pipette nozzle, for example, the pipette can be a sampling needle, so that the sampling needle is driven by the driving unit to move to absorb the sample to be tested from the sample container containing the blood sample.

[0072] The sample preparation device 12 includes a reaction cell and a reagent supply unit. The reaction cell is used to receive a portion of the sample to be tested drawn by the sampling device. The reagent supply unit provides a processing reagent to the reaction cell. The portion of the sample to be tested drawn by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction cell to prepare a sample solution to be tested.

[0073] The reagent supply unit includes at least a first reagent supply unit for supplying a hemolytic agent and a second reagent supply unit for supplying a diluent. In some application scenarios, the first reagent supply unit and the second reagent supply unit may also optionally supply a hemoglobin reagent, such as a hemolytic agent capable of lysing red blood cells in a blood sample, releasing hemoglobin within the red blood cells, and converting the hemoglobin into methemoglobin. When the first reagent and the second reagent are the same reagent, the first reagent supply unit and the second reagent supply unit constitute the same reagent supply unit.

[0074] A colorimetric detection device 13, configured to perform a hemoglobin test on a first sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first sample solution is prepared from a portion of the sample solution and a first reagent;

[0075] There are many methods for implementing the colorimetric method for hemoglobin detection, such as direct colorimetric method, Salisbury colorimetric method (indirect colorimetric method) and photoelectric colorimetric method.

[0076] an optical detection device 14 for performing a hemoglobin test on a second sample solution prepared by the sample preparation device using a light scattering method to obtain a second measurement value, wherein the second sample solution is prepared from a portion of the sample solution and a second reagent;

[0077] Among them, the light scattering method can refer to irradiating the second sample liquid to be tested with a laser light source with a certain wavelength, and then collecting optical information generated by particles in the second sample liquid to be tested after irradiation by the laser light source. The optical information can be, for example, scattered light information and fluorescence intensity information. Finally, based on the optical information, the number of red blood cell particles and the hemoglobin content of each red blood cell particle are counted to obtain a second measurement value.

[0078] The processor 15 is electrically connected to the optical detection device 14 and the colorimetric detection device 13, and is configured to:

[0079] Obtaining a first measurement value and a second measurement value from a colorimetric detection device and an optical detection device;

[0080] Determine whether the sample to be tested is a sample with falsely high hemoglobin;

[0081] If the sample to be tested is a sample with falsely high hemoglobin, output a second measurement value;

[0082] If the sample to be tested is not a sample with falsely high hemoglobin, the first measurement value is output.

[0083] In other embodiments of the present application, the optical detection device is further used to perform white blood cell detection on a third test sample solution prepared by the sample preparation device using a light scattering method, where the third test sample solution is prepared from a portion of the test sample and a third reagent;

[0084] The reagent supply unit also includes a third reagent supply unit for supplying leukocyte reagents. The leukocyte reagents include, for example, hemolytic agents that can dissolve red blood cells in a blood sample and distinguish different types of leukocytes, and further include fluorescent reagents that can stain leukocytes.

[0085] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0086] Acquiring first scattered light information detected when performing white blood cell detection on the third sample liquid from an optical detection device;

[0087] It is determined whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0088] The first scattered light information includes forward scattered light information and side scattered light information obtained during white blood cell detection. It should be noted that when using a light scattering method to detect hemoglobin in a second test sample to obtain a second measurement value, it is also possible to determine whether the test sample is a sample with falsely elevated hemoglobin levels based on optical information generated by corresponding particles when the second test sample is irradiated with a laser, such as the corresponding forward scattered light information and side scattered light information. In some application scenarios, the forward scattered light information and side scattered light information can also be used to determine the number of white blood cell particles and / or basophils in the test sample.

[0089] In other embodiments of the present application, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information, and the execution includes the following steps:

[0090] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0091] If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, it is determined that the sample to be tested is a chyle blood sample; wherein the sample with falsely high hemoglobin includes a chyle blood sample;

[0092] If the number of scattered particles included in the preset area of ​​the scattergram is less than the first preset number, it is determined that the sample to be tested is not a chylous blood sample.

[0093] In other embodiments of the present application, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0094] Calculating the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value;

[0095] If the mean red blood cell hemoglobin concentration is less than a preset threshold, it is determined that the sample to be tested is not a chylous blood sample; wherein, samples with falsely high hemoglobin levels include chylous blood samples.

[0096] In an embodiment of the present application, the blood analyzer further includes an impedance detection device for performing an impedance detection on a fourth test sample solution prepared by the sample preparation device to obtain a red blood cell count and a mean red blood cell volume using an impedance method, wherein the fourth test sample solution is prepared from a portion of the test sample and a fourth reagent;

[0097] The reagent supply unit further includes a fourth reagent supply unit for supplying a red blood cell reagent, such as a diluent.

[0098] Correspondingly, the processor is configured to calculate the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, and the execution includes the following steps:

[0099] The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0100] In other embodiments of the present application, the processor is configured to calculate the mean corpuscular hemoglobin concentration in the sample to be tested based on the first measurement value, and the calculation includes the following steps:

[0101] Acquiring, from the optical detection device, second scattered light information detected when performing hemoglobin detection on the second sample liquid to be tested;

[0102] determining the number of red blood cells and the average volume of the red blood cells in the sample to be tested based on the second scattered light information;

[0103] The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0104] In other embodiments of the present application, the processor is further configured to perform the following steps:

[0105] If the mean corpuscular hemoglobin concentration is greater than or equal to a predetermined threshold, calculating a difference between the first measurement value and the second measurement value;

[0106] If the difference is within a first preset range, it is determined that the sample to be tested is not a chylous blood sample;

[0107] If the difference is not within the first preset range, it is determined that the sample to be tested is a chylous blood sample.

[0108] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin:

[0109] Obtaining a blood smear image through a cell morphology analyzer, and analyzing the blood smear image to determine whether the sample to be tested is a chylomicron blood sample; wherein the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested through the cell morphology analyzer, the sample with falsely high hemoglobin level includes a chylomicron blood sample, and the blood analyzer includes a cell morphology analyzer;

[0110] Alternatively, an image of the test tube appearance is obtained by a camera system, and the image of the test tube appearance is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the image of the test tube appearance is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by the camera system, and the blood analyzer includes a camera system;

[0111] Alternatively, a bilirubin test result is obtained by a biochemical instrument, and the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the bilirubin test result is obtained by testing a portion of the sample to be tested by a biochemical instrument, a falsely high hemoglobin sample includes a high bilirubin sample, and a blood analyzer includes a biochemical instrument;

[0112] Alternatively, the blood lipid concentration test result is obtained from a biochemical instrument, and the blood lipid concentration test result is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the blood lipid concentration test result is obtained by testing a part of the sample to be tested by a biochemical instrument, and the blood analyzer includes a biochemical instrument.

[0113] In the embodiment of the present application, in addition to the aforementioned structural devices, the blood analyzer may also include one or more of a cell morphology analyzer, a camera system, and a biochemical instrument.

[0114] In other embodiments of the present application, Figure 2 As shown, the blood analyzer further includes a communication device 16 for communicating with an external device so as to obtain information from the external device as to whether the sample to be tested is a sample with falsely high hemoglobin;

[0115] In an embodiment of the present application, the external device may include a sample analyzer or a medical management system. Accordingly, the blood analyzer receives the test result of whether the sample to be tested is a sample with falsely high hemoglobin levels sent by the sample analyzer or the medical management system through a communication device, and sends the test result to the processor; wherein the sample analyzer is used to detect whether the sample to be tested is a sample with falsely high hemoglobin levels, and the detection method can refer to the aforementioned embodiment; or, the medical management system is used to store the test results.

[0116] The test results stored in the medical management system can be the ones sent by the sample analyzer to the medical management system. Correspondingly, in some application scenarios, the test results can be the original measurement information (such as blood smear image, test tube appearance image, bilirubin test result or blood lipid concentration test result) used to determine whether the sample to be tested is a sample with falsely high hemoglobin. In this way, after the blood sample analyzer obtains the test results from the medical management system, it is necessary to judge and analyze the test results to determine whether the sample to be tested is a sample with falsely high hemoglobin. In other application scenarios, the test results can also be the specific judgment result of whether the sample to be tested is a sample with falsely high hemoglobin after the sample analyzer or the medical management system analyzes the original measurement information of whether the sample to be tested is a sample with falsely high hemoglobin. In this way, after the blood sample analyzer obtains the test results from the medical management system, it is not necessary to judge and analyze the test results, and it can be directly known whether the sample to be tested is a sample with falsely high hemoglobin based on the test results. Among them, the sample analyzer and the cell morphology analyzer, the camera system and the biochemical instrument can be independent instruments that communicate directly or through the medical management system; or, the sample analyzer can include one or more of the cell morphology analyzer, the camera system and the biochemical instrument, that is, an all-in-one machine.

[0117] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0118] Based on the information from the external device, it is determined whether the sample to be tested is a sample with falsely high hemoglobin.

[0119] In other embodiments of the present application, the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from an external device:

[0120] Analyzing a blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes a blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin levels includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0121] Alternatively, analyzing the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, and the test tube appearance image is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes a camera system;

[0122] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0123] In an embodiment of the present application, the blood analyzer can also obtain the bilirubin test results corresponding to the sample to be tested from the medical management system, that is, after the biochemical instrument detects the bilirubin test results of the sample to be tested, the test results are uploaded to the medical management system so that they can be obtained from the medical management system when needed.

[0124] In other embodiments of the present application, the external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device:

[0125] Analyze the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, samples with falsely elevated hemoglobin levels include chylomicron samples, the blood smear image is a blood smear made from a portion of the sample to be tested photographed by a cell morphology analyzer and then sent to the medical management system, the test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested photographed by a camera system and then sent to the medical management system, and the blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and then sends it to the medical management system;

[0126] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; among which, samples with falsely high hemoglobin levels include high bilirubin samples, and the bilirubin test results are sent to the medical management system after being tested on a part of the sample to be tested by a biochemical instrument.

[0127] In the embodiment of the present application, the blood analyzer can also directly obtain the test results indicating the blood lipid concentration from the biochemical instrument through the communication device to determine whether the sample to be tested is a chylous blood sample.

[0128] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information:

[0129] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0130] Count the number of white blood cell particles in the scatter plot;

[0131] If the white blood cell count is greater than or equal to the second preset number, the sample to be tested is determined to be a high white blood cell count sample; wherein, a falsely high hemoglobin sample includes a high white blood cell count sample;

[0132] If the white blood cell count is less than the second predetermined number, it is determined that the sample to be tested is not a high white blood cell count sample.

[0133] The blood analyzer provided in the embodiment of the present application detects the hemoglobin in the test sample by a colorimetric method to obtain a first measurement value, and detects the hemoglobin in the test sample by a scattered light method to obtain a second measurement value, and then automatically determines whether the test sample is a sample with falsely high hemoglobin levels, and outputs the second measurement value if the test sample is a sample with falsely high hemoglobin levels, or outputs the first measurement value if the test sample is not a sample with falsely high hemoglobin levels. In this way, the hemoglobin content of the test sample is detected using an optical detection method and a colorimetric method respectively, and whether the test sample is a sample with falsely elevated hemoglobin is automatically determined. If the test sample is a sample with falsely elevated hemoglobin, a second measurement value obtained by performing hemoglobin detection on a second test sample solution prepared from a portion of the test sample using a light scattering method is output. Alternatively, if the test sample is not a sample with falsely elevated hemoglobin, a first measurement value obtained by performing hemoglobin detection on a first test sample solution prepared from a portion of the test sample using a colorimetric method is output. This solves the current problem of low hemoglobin testing efficiency caused by the need for manual determination of whether the test sample is a sample with falsely elevated hemoglobin. It enables the simultaneous detection of hemoglobin content in the test sample using different detection methods and the acquisition of corresponding test results. After automatically determining whether the test sample is a sample with falsely elevated hemoglobin and obtaining a determination result, the detection result obtained by the corresponding detection method is output based on the determination result. This ensures the accuracy of the hemoglobin concentration test result, shortens the detection process, and effectively increases the reliability of the hemoglobin concentration test result output by the blood analyzer.

[0134] The embodiment of the present application provides a blood analyzer, which includes: a sampling device, a sample preparation device, a colorimetric detection device, an optical detection device, and a processor; wherein:

[0135] The sampling device comprises a pipette and a driving unit, wherein the driving unit is used to drive the pipette to suck the sample to be tested;

[0136] The sample preparation device comprises a reaction pool and a reagent supply unit, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply unit provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction pool to prepare a sample liquid to be tested;

[0137] A colorimetric detection device for detecting hemoglobin in a sample using a colorimetric method;

[0138] An optical detection device for detecting hemoglobin in a sample using a light scattering method;

[0139] A processor is electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and is configured to:

[0140] Determine whether the sample to be tested is a sample with falsely high hemoglobin;

[0141] If the sample to be tested is not a sample with falsely high hemoglobin, controlling the sample preparation device to prepare a portion of the sample to be tested with a first reagent to obtain a first sample solution to be tested, and controlling the colorimetric detection device to perform a hemoglobin test on the first sample solution to obtain a first measurement value;

[0142] If the sample to be tested is a sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample solution to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample solution to obtain a second measurement value.

[0143] In other embodiments of the present application, the optical detection device is further used to perform white blood cell detection on a third test sample solution prepared by the sample preparation device using a light scattering method, where the third test sample solution is prepared from a portion of the test sample and a third reagent;

[0144] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0145] Acquiring first scattered light information detected when performing white blood cell detection on the third sample liquid from an optical detection device;

[0146] It is determined whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0147] In other embodiments of the present application, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information, and the execution includes the following steps:

[0148] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0149] If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, it is determined that the sample to be tested is a chyle blood sample; wherein the sample with falsely high hemoglobin includes a chyle blood sample;

[0150] If the number of scattered particles included in the preset area of ​​the scattergram is less than the first preset number, it is determined that the sample to be tested is not a chylous blood sample.

[0151] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin:

[0152] Obtaining a blood smear image through a cell morphology analyzer, and analyzing the blood smear image to determine whether the sample to be tested is a chylomicron blood sample; wherein the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested through the cell morphology analyzer, the sample with falsely high hemoglobin level includes a chylomicron blood sample, and the blood analyzer includes a cell morphology analyzer;

[0153] Alternatively, an image of the test tube appearance is obtained by a camera system, and the image of the test tube appearance is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the image of the test tube appearance is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by the camera system, and the blood analyzer includes a camera system;

[0154] Alternatively, a bilirubin test result is obtained by a biochemical instrument, and the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the bilirubin test result is obtained by testing a portion of the sample to be tested by a biochemical instrument, a falsely high hemoglobin sample includes a high bilirubin sample, and a blood analyzer includes a biochemical instrument;

[0155] Alternatively, the blood lipid concentration test result is obtained from a biochemical instrument, and the blood lipid concentration test result is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the blood lipid concentration test result is obtained by testing a part of the sample to be tested by a biochemical instrument, and the blood analyzer includes a biochemical instrument.

[0156] In other embodiments of the present application, the blood analyzer further includes a communication device for communicating with an external device so as to obtain information from the external device as to whether the sample to be tested is a sample with falsely high hemoglobin;

[0157] In an embodiment of the present application, the external device may include a sample analyzer or a medical management system. Correspondingly, the blood analyzer receives a test result of whether the sample to be tested is a sample with falsely high hemoglobin levels, sent by the sample analyzer or the medical management system, through a communication device, and sends the test result to the processor; wherein the sample analyzer is used to detect whether the sample to be tested is a sample with falsely high hemoglobin levels, or the medical management system is used to store the test result;

[0158] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and perform the following steps during execution:

[0159] Based on the information from the external device, it is determined whether the sample to be tested is a sample with falsely high hemoglobin.

[0160] In other embodiments of the present application, the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from an external device:

[0161] Analyzing a blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes a blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin levels includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0162] or,

[0163] Analyzing the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, which is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes a camera system;

[0164] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0165] In other embodiments of the present application, the external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device:

[0166] Analyze the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, samples with falsely elevated hemoglobin levels include chylomicron samples, the blood smear image is a blood smear made from a portion of the sample to be tested photographed by a cell morphology analyzer and then sent to the medical management system, the test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested photographed by a camera system and then sent to the medical management system, and the blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and then sends it to the medical management system;

[0167] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; among which, samples with falsely high hemoglobin levels include high bilirubin samples, and the bilirubin test results are sent to the medical management system after being tested on a part of the sample to be tested by a biochemical instrument.

[0168] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information:

[0169] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0170] Count the number of white blood cell particles in the scatter plot;

[0171] If the white blood cell count is greater than or equal to the second preset number, the sample to be tested is determined to be a high white blood cell count sample; wherein, a falsely high hemoglobin sample includes a high white blood cell count sample;

[0172] If the white blood cell count is less than the second predetermined number, it is determined that the sample to be tested is not a high white blood cell count sample.

[0173] The blood analyzer provided in the embodiment of the present application first determines whether the sample to be tested is a sample with falsely elevated hemoglobin levels. Then, if the sample to be tested is not a sample with falsely elevated hemoglobin levels, a first sample liquid to be tested is obtained and a hemoglobin test is performed on the first sample liquid to be tested using a colorimetric method to obtain a first measurement value. Alternatively, if the sample to be tested is a sample with falsely elevated hemoglobin levels, a second sample liquid to be tested is obtained and a hemoglobin test is performed on the second sample liquid to be tested using a light scattering method to obtain a second measurement value. In this way, it is automatically judged whether the sample to be tested is a sample with falsely high hemoglobin, and a judgment result is obtained. The corresponding detection method is selected according to the judgment result to detect the hemoglobin of the sample to be tested and the corresponding detection result is output. This solves the current problem of low efficiency in hemoglobin testing due to the need for manual judgment of whether the sample to be tested is a sample with falsely high hemoglobin. It realizes the selective selection of the corresponding detection method to detect the hemoglobin of the sample to be tested according to the judgment result of whether the sample to be tested is a sample with falsely high hemoglobin, effectively improving the accuracy of the hemoglobin detection result and increasing the reliability of the hemoglobin detection result output by the blood analyzer.

[0174] The embodiment of the present application provides a blood analyzer, which includes: a sampling device, a sample preparation device, a colorimetric detection device, an optical detection device, and a processor; wherein:

[0175] The sampling device comprises a pipette and a driving unit, wherein the driving unit is used to drive the pipette to suck the sample to be tested;

[0176] The sample preparation device comprises a reaction pool and a reagent supply unit, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply unit provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction pool to prepare a sample liquid to be tested;

[0177] a colorimetric detection device for performing a hemoglobin test on a first test sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first test sample solution is prepared from a portion of the test sample and a first reagent;

[0178] An optical detection device for detecting hemoglobin in a sample using a light scattering method;

[0179] A processor is electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and is configured to:

[0180] Determine whether the sample to be tested is a sample with falsely high hemoglobin;

[0181] If the sample to be tested is not a sample with falsely high hemoglobin, output the first measurement value;

[0182] If the sample to be tested is a sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample liquid to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample liquid to obtain a second measurement value, and the second measurement value is output.

[0183] In other embodiments of the present application, the optical detection device is further used to perform white blood cell detection on a third test sample solution prepared by the sample preparation device using a light scattering method, where the third test sample solution is prepared from a portion of the test sample and a third reagent;

[0184] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0185] Acquiring first scattered light information detected when performing white blood cell detection on the third sample liquid from an optical detection device;

[0186] It is determined whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0187] In other embodiments of the present application, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information, and the execution includes the following steps:

[0188] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0189] If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, it is determined that the sample to be tested is a chyle blood sample; wherein the sample with falsely high hemoglobin includes a chyle blood sample;

[0190] If the number of scattered particles included in the preset area of ​​the scattergram is less than the first preset number, it is determined that the sample to be tested is not a chylous blood sample.

[0191] In an embodiment of the present application, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0192] Calculating the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value;

[0193] If the mean red blood cell hemoglobin concentration is less than a preset threshold, it is determined that the sample to be tested is not a chylous blood sample; wherein, samples with falsely high hemoglobin levels include chylous blood samples.

[0194] In other embodiments of the present application, the blood analyzer further includes an impedance detection device for performing an impedance detection on a fourth test sample solution prepared by the sample preparation device to obtain a red blood cell count and a mean red blood cell volume using an impedance method, wherein the fourth test sample solution is prepared from a portion of the test sample and a fourth reagent;

[0195] Correspondingly, the processor is configured to calculate the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, and the execution includes the following steps:

[0196] The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0197] In other embodiments of the present application, the processor is configured to calculate the mean corpuscular hemoglobin concentration in the sample to be tested based on the first measurement value, and the calculation includes the following steps:

[0198] Acquiring, from the optical detection device, second scattered light information detected when performing hemoglobin detection on the second sample liquid to be tested;

[0199] determining the number of red blood cells and the average volume of the red blood cells in the sample to be tested based on the second scattered light information;

[0200] The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0201] In other embodiments of the present application, the processor is further configured to perform the following steps:

[0202] If the mean corpuscular hemoglobin concentration is greater than or equal to a predetermined threshold, calculating a difference between the first measurement value and the second measurement value;

[0203] If the difference is within a first preset range, it is determined that the sample to be tested is not a chylous blood sample;

[0204] If the difference is not within the first preset range, it is determined that the sample to be tested is a chylous blood sample.

[0205] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin:

[0206] Obtaining a blood smear image through a cell morphology analyzer, and analyzing the blood smear image to determine whether the sample to be tested is a chylomicron blood sample; wherein the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested through the cell morphology analyzer, the sample with falsely high hemoglobin level includes a chylomicron blood sample, and the blood analyzer includes a cell morphology analyzer;

[0207] Alternatively, an image of the test tube appearance is obtained by a camera system, and the image of the test tube appearance is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the image of the test tube appearance is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by the camera system, and the blood analyzer includes a camera system;

[0208] Alternatively, a bilirubin test result is obtained by a biochemical instrument, and the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the bilirubin test result is obtained by testing a portion of the sample to be tested by a biochemical instrument, a falsely high hemoglobin sample includes a high bilirubin sample, and a blood analyzer includes a biochemical instrument;

[0209] Alternatively, the blood lipid concentration test result is obtained from a biochemical instrument, and the blood lipid concentration test result is analyzed to determine whether the sample to be tested is a chylous blood sample; wherein the blood lipid concentration test result is obtained by testing a part of the sample to be tested by a biochemical instrument, and the blood analyzer includes a biochemical instrument.

[0210] In other embodiments of the present application, the blood analyzer further includes a communication device for communicating with an external device so as to obtain information from the external device as to whether the sample to be tested is a sample with falsely high hemoglobin;

[0211] In an embodiment of the present application, the external device may include a sample analyzer or a medical management system. Correspondingly, the blood analyzer receives a test result of whether the sample to be tested is a sample with falsely high hemoglobin levels, sent by the sample analyzer or the medical management system, through a communication device, and sends the test result to the processor; wherein the sample analyzer is used to detect whether the sample to be tested is a sample with falsely high hemoglobin levels, or the medical management system is used to store the test result;

[0212] Correspondingly, the processor is configured to determine whether the sample to be tested is a sample with falsely high hemoglobin, and the execution includes the following steps:

[0213] Based on the information from the external device, it is determined whether the sample to be tested is a sample with falsely high hemoglobin.

[0214] In other embodiments of the present application, the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from an external device:

[0215] Analyzing a blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes a blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin levels includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0216] Alternatively, analyzing the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, and the test tube appearance image is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes a camera system;

[0217] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0218] In other embodiments of the present application, the external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device:

[0219] Analyze the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, samples with falsely elevated hemoglobin levels include chylomicron samples, the blood smear image is a blood smear made from a portion of the sample to be tested photographed by a cell morphology analyzer and then sent to the medical management system, the test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested photographed by a camera system and then sent to the medical management system, and the blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and then sends it to the medical management system;

[0220] Alternatively, the bilirubin test results are analyzed to determine whether the sample to be tested is a high bilirubin sample; among which, samples with falsely high hemoglobin levels include high bilirubin samples, and the bilirubin test results are sent to the medical management system after being tested on a part of the sample to be tested by a biochemical instrument.

[0221] In other embodiments of the present application, the processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin levels based on the first scattered light information:

[0222] generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information;

[0223] Count the number of white blood cell particles in the scatter plot;

[0224] If the white blood cell count is greater than or equal to the second preset number, the sample to be tested is determined to be a high white blood cell count sample; wherein, a falsely high hemoglobin sample includes a high white blood cell count sample;

[0225] If the white blood cell count is less than the second predetermined number, it is determined that the sample to be tested is not a high white blood cell count sample.

[0226] The blood analyzer provided in the embodiment of the present application first obtains a first sample liquid to be tested, and performs a hemoglobin test on the first sample liquid to be tested using a colorimetric method to obtain a first measurement value. Then, it is determined whether the sample to be tested is a sample with falsely high hemoglobin levels. Finally, if the sample to be tested is not a sample with falsely high hemoglobin levels, the first measurement value is output. Alternatively, if the sample to be tested is a sample with falsely high hemoglobin levels, a second sample liquid to be tested is obtained and a hemoglobin test is performed on the second sample liquid to be tested using a light scattering method to obtain a second measurement value. In this way, the hemoglobin of the sample to be tested is first detected by the colorimetric method, and then it is automatically determined whether the sample to be tested is a sample with falsely high hemoglobin. When the sample to be tested is not a sample with falsely high hemoglobin, the first measurement value obtained by the hemoglobin detection of the sample to be tested by the colorimetric method is directly output. When the sample to be tested is a sample with falsely high hemoglobin, the hemoglobin of the sample to be tested is detected by the light scattering method, and the corresponding detection result is output. This solves the problem of low efficiency of hemoglobin testing caused by manual judgment of whether the sample to be tested is a sample with falsely high hemoglobin, and realizes that after the hemoglobin of the sample to be tested is detected by the colorimetric method, If the automated test sample is not a sample with falsely high hemoglobin levels, the test result of the colorimetric method is directly output, thereby improving the output efficiency of the test result; if the automated test sample is a sample with falsely high hemoglobin levels, the light scattering method is continued to be used to detect the hemoglobin in the sample. Since the hemoglobin concentration result in the sample with falsely high hemoglobin levels detected by the light scattering method is more accurate than the hemoglobin concentration detected by the colorimetric method, the accuracy of the hemoglobin concentration test result is effectively guaranteed when the sample with falsely high hemoglobin levels is a sample with falsely high hemoglobin levels. In this way, the reliability of the hemoglobin concentration test result of the blood analyzer is effectively improved.

[0227] The present invention provides a blood analysis method. Figure 3As shown, the method is applied to a blood analyzer, which includes a control device, a sampling device, a sample preparation device, a colorimetric detection device, and an optical detection device, wherein the control device is the aforementioned processor. The method includes the following steps:

[0228] Step 201: The control device controls the sampling device to obtain a sample to be tested.

[0229] In the embodiment of the present application, the sample to be tested may refer to a blood sample of a patient who needs to undergo a blood test.

[0230] Step 202: The control device controls the sample preparation device to mix a portion of the sample to be tested with the first reagent to prepare a first sample solution to be tested, and to mix a portion of the sample to be tested with the second reagent to prepare a second sample solution to be tested.

[0231] In an embodiment of the present application, the sample preparation device obtains part of the sample from the sample to be tested, obtaining at least a first sample and a second sample, and mixes the first sample with a first reagent to prepare a first sample liquid to be tested, and mixes the second sample with a second reagent to prepare a second sample liquid to be tested.

[0232] Depending on the sample preparation requirements, different amounts of partial samples can be obtained from the sample to be tested to obtain a first sample and a second sample. The first reagent can be a hemoglobin reagent, which is, for example, a hemolytic agent capable of lysing red blood cells in a blood sample, releasing hemoglobin in the red blood cells, and converting the hemoglobin into methemoglobin. The second reagent can also be a hemoglobin reagent, that is, the first and second reagents can be the same.

[0233] Step 203: The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample solution using the colorimetric method to obtain a first measurement value.

[0234] Step 204: The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid using a light scattering method to obtain a second measurement value.

[0235] In the embodiment of the present application, the scattered light information of the red blood cell channel in the blood sample analyzer can be used to implement the hemoglobin detection of the second sample liquid to be tested.

[0236] Step 205: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin.

[0237] In an embodiment of the present application, determining whether a sample to be tested is a sample with falsely high hemoglobin levels can be achieved based on scattered light intensity information in any blood routine measurement channel in the blood analyzer, including forward scattered light intensity information and side scattered light intensity information, wherein any blood routine measurement channel can be a red blood cell detection channel or a white blood cell detection channel, etc.; it can also be achieved by the blood analyzer communicating with a detection device used to detect whether a blood sample is a sample with falsely high hemoglobin levels, such as a film reader, and obtaining the corresponding test results from the detection device; it can also be achieved by the blood analyzer obtaining the user identity information corresponding to the sample to be tested from the hospital's medical management system, such as the Laboratory Information System (LIS). The communication method between the blood analyzer and the detection device, such as the film reader, includes but is not limited to the Internet, Bluetooth, broadcast, etc. The communication method between the blood analyzer and the medical management system can be achieved through the Internet between the blood analyzer and the server corresponding to the medical management system. Samples with falsely high hemoglobin levels include at least one of the following: chylous blood samples, high white blood cell particle samples, high bilirubin samples, etc.

[0238] It should be noted that after executing step 205, the blood analyzer can choose to execute step 206 or step 207. If the sample to be tested is a sample with falsely high hemoglobin, step 206 is executed; if the sample to be tested is not a sample with falsely high hemoglobin, step 207 is executed.

[0239] Step 206: If the sample to be tested is a sample with falsely high hemoglobin, the control device outputs a second measurement value.

[0240] Step 207: If the sample to be tested is not a sample with falsely high hemoglobin, the control device outputs a first measurement value.

[0241] Based on the above embodiment, in other embodiments of the present application, before the blood analyzer executes step 205, it may further execute step 208:

[0242] Step 208: The control device controls the sample preparation device to mix a portion of the test sample with the third reagent to prepare a third test sample solution. The control device also controls the sample preparation device to transport the third test sample solution to the optical detection device so that the optical detection device performs white blood cell detection on the third test sample solution using a light scattering method.

[0243] In the embodiment of the present application, the control device controls the sample preparation device to obtain a portion of the sample from the sample to be tested to obtain a third sample, and mixes the third sample with a third reagent to prepare a third sample liquid to be tested.

[0244] Correspondingly, step 205 can be specifically implemented by steps 205a to 205b:

[0245] Step 205a: The control device obtains first scattered light information detected when performing white blood cell detection on the third sample liquid.

[0246] Step 205b: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0247] In other embodiments of the present application, step 205b may be implemented by steps a11 to a13:

[0248] Step a11: The control device generates a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0249] Step a12: If the number of scattered particles included in the preset area of ​​the scattergram is greater than or equal to a first preset number, the control device determines that the sample to be tested is a chylous blood sample.

[0250] Among them, samples with falsely high hemoglobin levels include chylous blood samples.

[0251] In the embodiment of the present application, the first preset number is an empirical value determined based on a large number of experiments or actual applications, which can be corrected during actual application, or can be a value set by the user according to actual needs. For example, Figure 4 According to the scatter plot obtained from the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information, the number of scattered particles in the preset area A is counted, and it is determined that the number of scattered particles in the preset area A is greater than the first preset number. Therefore, it can be determined that the sample to be tested is a chylous blood sample. Figure 4 In the figure, the ordinate is the signal intensity of the forward scattered light signal (Front Scattering, FS) in the first scattered light information, and the ordinate is the signal intensity of the side scattered light signal (Side Scattering, SS) in the first scattered light information.

[0252] It should be noted that, in some application scenarios, the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information can also be used to count the number of white blood cell particles and / or basophils in the sample to be tested.

[0253] Step a13: If the number of scattered particles included in the preset area of ​​the scattergram is less than a first preset number, the control device determines that the sample to be tested is not a chylous blood sample.

[0254] For example, if the scatter plot obtained based on the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information is as follows Figure 5 As shown, the number of scattered particles in the preset area B is counted, and it is determined that the number of scattered particles in the preset area B is less than the first preset number. Therefore, it can be determined that the sample to be tested is not a chylous blood sample.

[0255] It should be noted that in the embodiment consisting of steps 201-204, step 208, steps 205a-205b, and steps 206-207 executed in sequence, or in the embodiment consisting of steps 201-204, step 208, steps a11-a13, step 205b, and steps 206-207 executed in sequence, the corresponding blood sample analyzer has a white blood cell detection channel. Thus, while the blood analyzer is performing white blood cell detection on the sample to be tested, it obtains first scattered light information detected during the white blood cell detection to determine whether the sample to be tested is chylous blood, obtains a determination result, and then outputs a corresponding hemoglobin measurement value based on the corresponding determination result. This ensures the accuracy of the output hemoglobin measurement value and improves the intelligence level of the blood analyzer.

[0256] In other embodiments of the present application, step 205b may also be implemented by steps a14 to a17:

[0257] Step a14: generating a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0258] Step a15: Count the number of white blood cell particles in the scatter plot.

[0259] Step a16: If the number of white blood cell particles is greater than or equal to the second preset number, determine that the sample to be tested is a high white blood cell particle sample.

[0260] Among them, samples with falsely high hemoglobin levels include samples with high white blood cell particles.

[0261] Step a17: If the number of white blood cell particles is less than the second preset number, it is determined that the sample to be tested is not a high white blood cell particle sample.

[0262] Based on the above embodiment, in other embodiments of the present application, step 205 may be implemented by steps 205c to 205g only after the hemoglobin of the sample to be tested is measured by a colorimetric method to obtain a first measurement value:

[0263] Step 205c: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value.

[0264] In the embodiment of the present application, only after the hemoglobin of the sample to be tested is measured by colorimetry,

[0265] It should be noted that after the blood analyzer executes step 205c, it can choose to execute step 205d or steps 205e to 205g. If the mean corpuscular hemoglobin concentration is less than the preset threshold, the blood analyzer chooses to execute step 205d; if the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, the blood analyzer chooses to execute steps 205e to 205g:

[0266] Step 205d: If the mean corpuscular hemoglobin concentration is less than the preset threshold, the control device determines that the sample to be tested is not a chylous blood sample.

[0267] In an embodiment of the present application, the preset threshold is an empirical value. Since different hospitals have different judgment rules, the preset threshold for different hospitals may be different, or the preset threshold is a pre-set empirical value, but in actual application, it can be continuously corrected and modified.

[0268] Step 205e: If the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, the control device calculates the difference between the first measurement value and the second measurement value.

[0269] After executing step 205e, the blood analyzer may choose to execute step 205f or step 205g. If the difference is within the first preset range, step 205f is executed; if the difference is within the second preset range, step 205g is executed.

[0270] Step 205f: If the difference is within the first preset range, the control device determines that the sample to be tested is not a chylous blood sample.

[0271] Step 205g: If the difference is within the second preset range, the control device determines that the sample to be tested is a chylous blood sample.

[0272] In an embodiment of the present application, in some application scenarios, the blood analyzer can determine that the sample to be tested is a chylosing blood sample when the mean corpuscular hemoglobin concentration is greater than or equal to a preset threshold value. However, the increase in the mean corpuscular hemoglobin concentration may be caused by aggregation. Therefore, in some application scenarios, it is necessary to further exclude the possibility that the increase in the mean corpuscular hemoglobin concentration is caused by red blood cell aggregation. The method for further exclusion is: comparing a first measurement value of hemoglobin obtained by colorimetry of the sample to be tested with a second measurement value of hemoglobin obtained by light scattering of the sample to be tested. For example, the difference between the first measurement value and the second measurement value can be calculated, and then the preset range corresponding to the difference can be determined, thereby eliminating the problem that the mean corpuscular hemoglobin concentration is increased due to red blood cell aggregation, leading to the misjudgment of the sample to be tested as a chylosing blood sample, thereby improving the accuracy of accurately determining that the sample to be tested is a chylosing blood sample.

[0273] In other embodiments of the present application, the number of red blood cells and the mean corpuscular volume of the sample to be tested may be obtained by an impedance method. That is, before the blood analyzer executes step 205c, steps 205h to 205i may also be executed:

[0274] Step 205h: The control device controls the sample preparation device to mix a portion of the sample to be tested with the fourth reagent to prepare a fourth sample solution to be tested.

[0275] In the embodiment of the present application, the control device controls the sample preparation device to obtain a portion of the sample from the sample to be tested to obtain a fourth sample, and mixes the fourth sample with a fourth reagent to prepare a fourth sample liquid to be tested.

[0276] Step 205i: The control device controls the sample preparation device to transport the fourth sample solution to be tested to the impedance detection device, so that the impedance detection device performs red blood cell detection on the fourth sample solution using the impedance method to obtain the red blood cell count and the mean red blood cell volume.

[0277] Correspondingly, step 205c can be specifically implemented by step b11:

[0278] Step b11: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0279] In the embodiment of the present application, the calculation formula for calculating the mean corpuscular hemoglobin concentration MCHC of the sample to be tested can be expressed as: Here, HGB is used to represent the first measurement value, RBC is used to represent the number of red blood cells, and MCV is used to represent the mean corpuscular volume.

[0280] Alternatively, in other embodiments of the present application, a light scattering method may be used to obtain the number of red blood cells and the average volume of red blood cells in the sample to be tested. Correspondingly, step 205c may also be implemented by steps b12 to b14:

[0281] Step b12: The control device obtains second scattered light information detected when performing hemoglobin detection on the second sample liquid to be tested.

[0282] Step b13: The control device determines the number of red blood cells and the average volume of the red blood cells in the sample to be tested based on the second scattered light information.

[0283] Step b14: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, the number of red blood cells and the mean corpuscular volume.

[0284] In the embodiment of the present application, the calculation formula for calculating the mean hemoglobin concentration of the red blood cells of the sample to be tested can refer to the calculation formula in step b11, and will not be repeated here.

[0285] In other embodiments of the present application, step 205 may also be implemented by the following steps:

[0286] The control device obtains a blood smear image through a cell morphology analyzer, analyzes the blood smear image, and determines whether the sample to be tested is a chylomicron blood sample; wherein the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested by the cell morphology analyzer, the sample with falsely high hemoglobin level includes a chylomicron blood sample, and the blood analyzer includes a cell morphology analyzer;

[0287] Alternatively, the control device acquires an image of the test tube appearance through a camera system, and analyzes the image of the test tube appearance to determine whether the sample to be tested is a chylous blood sample; wherein the image of the test tube appearance is obtained by photographing the appearance of a blood anticoagulant test tube containing a portion of the sample to be tested by the camera system, and the blood analyzer includes a camera system;

[0288] Alternatively, the control device obtains a bilirubin test result through a biochemical instrument, and analyzes the bilirubin test result to determine whether the sample to be tested is a high bilirubin sample; wherein the bilirubin test result is obtained by testing a portion of the sample to be tested by the biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the blood analyzer includes a biochemical instrument;

[0289] Alternatively, the control device obtains the blood lipid concentration test result from the biochemical instrument, and analyzes the blood lipid concentration test result to determine whether the sample to be tested is a chylous blood sample; wherein the blood lipid concentration test result is obtained by testing a part of the sample to be tested by the biochemical instrument, and the blood analyzer includes a biochemical instrument.

[0290] Based on the above embodiment, in other embodiments of the present application, the blood analyzer further includes a communication device, and step 205 can also be implemented through step 205j:

[0291] Step 205j: The control device controls the communication device to receive information sent by the external device indicating whether the sample to be processed is a sample with falsely high hemoglobin.

[0292] In an embodiment of the present application, the external device may be a sample analyzer or a medical management system. Accordingly, the blood analyzer receives a test result from the sample analyzer or the medical management system indicating whether the sample under test is a falsely elevated hemoglobin sample. The sample analyzer may further include one or more of a cell morphology analyzer, an imaging system, and a biochemical instrument.

[0293] The test result is obtained by a sample analyzer to determine whether the sample to be tested is a sample with falsely high hemoglobin, or the test result is stored in a medical management system.

[0294] The sample analyzer can be, for example, a film reader, i.e., a cell morphology analyzer. When the sample analyzer is a film reader, the sample to be tested must be processed to obtain a blood smear, which is then analyzed by the film reader to obtain the test results. The medical management system can be an intermediate management system used by a hospital to store various medical test results, such as a laboratory information system (LIS). The various test results in the LIS can be stored in the LIS after being tested using various testing methods. Accordingly, when the test result of whether the sample to be tested is chylous blood is needed, the corresponding test result in the LIS can be retrieved based on the patient's identification information corresponding to the sample to be tested.

[0295] Correspondingly, step 205j can be specifically implemented by the following steps: the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device.

[0296] In the embodiment of the present application, the test result can directly be: the sample to be tested is a sample with falsely high hemoglobin, or the sample to be tested is not a sample with falsely high hemoglobin, or the test result can be some parameters used to determine whether it is a sample with falsely high hemoglobin.

[0297] In other embodiments of the present application, step 205j may also be implemented by the following steps:

[0298] The control device analyzes the blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin level includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0299] Alternatively, the control device analyzes the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, and the test tube appearance image is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes a camera system;

[0300] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0301] In other embodiments of the present application, the external device includes a medical management system, and the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device by the following steps:

[0302] The control device analyzes the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, a sample with falsely elevated hemoglobin includes a chylomicron sample; the blood smear image is a blood smear prepared by a cell morphology analyzer and photographed and sent to the medical management system; the test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested is photographed and sent to the medical management system by a camera system; and the blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and sends the result to the medical management system;

[0303] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the falsely high hemoglobin sample includes a high bilirubin sample, and the bilirubin test results are sent to the medical management system after a part of the sample to be tested is tested by a biochemical instrument.

[0304] The blood analysis method provided in the embodiment of the present application adopts optical detection method and colorimetry method to detect the hemoglobin of the sample to be tested, and automatically determines whether the sample to be tested is a sample with falsely high hemoglobin. When the sample to be tested is a sample with falsely high hemoglobin, the method outputs a second measurement value obtained by performing hemoglobin detection on a second sample solution to be tested prepared by using light scattering method on a part of the sample to be tested, or when the sample to be tested is not a sample with falsely high hemoglobin, the method outputs a first measurement value obtained by performing hemoglobin detection on a first sample solution to be tested prepared by using colorimetry method on a part of the sample to be tested, The invention solves the problem that manual judgment is currently needed to determine whether the sample to be tested is a sample with falsely high hemoglobin, resulting in low efficiency in hemoglobin testing. It realizes the simultaneous use of different detection methods to detect the hemoglobin of the sample to be tested and obtains corresponding test results. After automatically judging whether the sample to be tested is a sample with falsely high hemoglobin and obtaining the judgment result, the test result obtained by the corresponding detection method is output according to the judgment result, thereby ensuring the accuracy of the hemoglobin concentration test result, shortening the detection process, and effectively increasing the reliability of the hemoglobin concentration test result output by the blood analyzer.

[0305] The present invention provides a blood analysis method. Figure 6 As shown, the method is applied to a blood analyzer, which includes a control device, a sampling device, a sample preparation device, a colorimetric detection device, and an optical detection device, wherein the control device is the aforementioned processor. The method includes the following steps:

[0306] Step 301: The control device controls the sampling device to obtain a sample to be tested.

[0307] Step 302: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin.

[0308] In this embodiment of the present application, after executing step 302, the blood analyzer can choose to execute steps 303-304 or steps 305-306. If the sample to be tested is not a sample with falsely elevated hemoglobin, steps 303-304 are executed; if the sample to be tested is a sample with falsely elevated hemoglobin, steps 305-306 are executed.

[0309] Step 303: If the sample to be tested is not a sample with falsely high hemoglobin, the control device controls the sample preparation device to mix part of the sample to be tested with the first reagent to prepare a first sample solution to be tested, and controls the sample preparation device to transport the first sample solution to be tested to the colorimetric detection device.

[0310] In the embodiment of the present application, if the sample to be tested is not a sample with falsely high hemoglobin, the control device controls the sample preparation device to obtain a portion of the sample to be tested to obtain a first sample, and mixes the first sample with a first reagent to prepare a first sample liquid to be tested.

[0311] Step 304: The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample solution using the colorimetric method to obtain a first measurement value.

[0312] Step 305: If the sample to be tested is a sample with falsely high hemoglobin, the control device controls the sample preparation device to mix part of the sample to be tested with the second reagent to prepare a second sample solution to be tested.

[0313] In an embodiment of the present application, if the sample to be tested is a sample with falsely high hemoglobin, a portion of the sample to be tested is obtained to obtain a second sample, and the second sample is mixed with a second reagent to prepare a second sample solution to be tested.

[0314] Step 306: The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid using a light scattering method to obtain a second measurement value.

[0315] Based on the above embodiment, in other embodiments of the present application, when the blood sample analyzer has a white blood cell detection channel, when the white blood cell detection channel detects the white blood cells of the sample to be tested, it can also determine whether the sample to be tested is a sample with falsely high hemoglobin. That is, before the blood analyzer executes step 302, it can also execute step 307:

[0316] Step 307 : The control device controls the sample preparation device to mix part of the sample to be tested with the third reagent to prepare a third sample solution to be tested.

[0317] In the embodiment of the present application, a portion of the sample is obtained from the sample to be tested to obtain a third sample, and the third sample is mixed with a third reagent to prepare a third sample solution to be tested.

[0318] Correspondingly, step 302 can be specifically implemented by steps 302a to 302b:

[0319] Step 302a: The control device obtains first scattered light information detected when performing white blood cell detection on the third sample liquid.

[0320] Step 302b: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0321] In other embodiments of the present application, step 302b may be implemented by steps c11 to c13:

[0322] Step c11: The control device generates a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0323] Step c12: If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, the control device determines that the sample to be tested is a chylomicron sample; wherein, the sample with falsely high hemoglobin level includes a chylomicron sample.

[0324] Step c13: If the number of scattered particles included in the preset area of ​​the scattergram is less than a first preset number, the control device determines that the sample to be tested is not a chylous blood sample.

[0325] In other embodiments of the present application, step 302b may also be implemented by steps c14 to c17:

[0326] Step c14: generating a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0327] Step c15: Count the number of white blood cell particles in the scatter plot.

[0328] Step c16: If the number of white blood cell particles is greater than or equal to the second preset number, determine that the sample to be tested is a high white blood cell particle sample.

[0329] Among them, samples with falsely high hemoglobin levels include samples with high white blood cell particles.

[0330] Step c17: If the number of white blood cell particles is less than the second preset number, it is determined that the sample to be tested is not a high white blood cell particle sample.

[0331] Based on the above embodiment, in other embodiments of the present application, the blood analyzer may further implement step 302 by performing step 302c. Correspondingly, the blood analyzer further includes a communication device:

[0332] Step 302c: The control device controls the communication device to receive information sent by an external device indicating whether the sample to be processed is a sample with falsely high hemoglobin.

[0333] In the embodiment of the present application, the external device may be a sample analyzer or a medical management system. Correspondingly, the blood analyzer receives the test result sent by the sample analyzer or the medical management system to determine whether the sample to be tested is a sample with falsely high hemoglobin.

[0334] The test result is obtained by a sample analyzer to determine whether the sample to be tested is a sample with falsely high hemoglobin, or the test result is stored in a medical management system.

[0335] Correspondingly, step 302c can be specifically implemented by the following steps: the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device.

[0336] In other embodiments of the present application, when the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from an external device, it can be specifically achieved by the following steps:

[0337] The control device analyzes the blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin level includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0338] Alternatively, the control device analyzes the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes: a test tube appearance image obtained by a camera system photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested, the external device including the camera system;

[0339] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0340] In other embodiments of the present application, the external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device:

[0341] Analyze the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, samples with falsely elevated hemoglobin levels include chylomicron samples. The blood smear image is a blood smear made from a portion of the sample to be tested by a cell morphology analyzer and then sent to the medical management system. The test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested by a camera system and then sent to the medical management system. The blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and then sends it to the medical management system.

[0342] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the falsely high hemoglobin sample includes a high bilirubin sample, and the bilirubin test results are sent to the medical management system after a part of the sample to be tested is tested by a biochemical instrument.

[0343] It should be noted that, for the description of the same steps and contents in this embodiment as those in other embodiments, reference can be made to the description in other embodiments and will not be repeated here.

[0344] The blood analysis method provided in the embodiment of the present application automatically determines whether the sample to be tested is a sample with falsely high hemoglobin levels, obtains a determination result, and selects a corresponding detection method based on the determination result to detect the hemoglobin of the sample to be tested and outputs the corresponding detection result. This solves the current problem of low efficiency in hemoglobin testing due to the need for manual determination of whether the sample to be tested is a sample with falsely high hemoglobin levels. This method selectively selects a corresponding detection method to detect the hemoglobin of the sample to be tested based on the determination result of whether the sample to be tested is a sample with falsely high hemoglobin levels, effectively improving the accuracy of the hemoglobin detection results and increasing the reliability of the hemoglobin detection results output by the blood analyzer.

[0345] The present invention provides a blood analysis method. Figure 7 As shown, the method is applied to a blood analyzer, which includes a control device, a sampling device, a sample preparation device, a colorimetric detection device, and an optical detection device, wherein the control device is the aforementioned processor. The method includes the following steps:

[0346] Step 401: The control device controls the sampling device to obtain a sample to be tested.

[0347] Step 402: The control device controls the sample preparation device to mix a portion of the sample to be tested with the first reagent to prepare a first sample solution to be tested.

[0348] In the embodiment of the present application, the control device controls the sample preparation device to obtain a portion of the sample from the sample to be tested to obtain a first sample, and mixes the first sample with a first reagent to prepare a first sample liquid to be tested.

[0349] Step 403: The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample solution using the colorimetric method to obtain a first measurement value.

[0350] Step 404: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin.

[0351] In the embodiment of the present application, after the blood sample analyzer executes step 404, it may choose to execute step 405, or choose to execute steps 406 to 407.

[0352] Step 405: If the sample to be tested is not a sample with falsely high hemoglobin, the control device outputs a first measurement value.

[0353] Step 406: If the sample to be tested is a sample with falsely high hemoglobin, the control device controls the sample preparation device to mix part of the sample to be tested with a second reagent to prepare a second sample solution to be tested, and controls the sample preparation device to transport the second sample solution to be tested to the optical detection device.

[0354] In the embodiment of the present application, if the sample to be tested is a sample with falsely high hemoglobin, the control device controls the sample preparation device to obtain a portion of the sample to be tested to obtain a second sample, and mixes the second sample with a second reagent to prepare a second sample solution to be tested.

[0355] Step 407: The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid using a light scattering method to obtain a second measurement value, and outputs the second measurement value.

[0356] Based on the above embodiment, in other embodiments of the present application, before the blood analyzer executes step 404, it may further execute step 408:

[0357] Step 408 : The control device controls the sample preparation device to mix part of the sample to be tested with the third reagent to prepare a third sample solution to be tested, and controls the sample preparation device to transport the third sample solution to be tested to the optical detection device.

[0358] In the embodiment of the present application, the control device controls the sample preparation device to obtain a portion of the sample from the sample to be tested to obtain a third sample, and mixes the third sample with a third reagent to prepare a third sample liquid to be tested.

[0359] Correspondingly, step 404 can be specifically implemented by steps 404a to 404b:

[0360] Step 404a: The control device obtains first scattered light information detected when performing white blood cell detection on the third sample liquid.

[0361] Step 404b: The control device determines whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

[0362] In other embodiments of the present application, step 404b may be implemented by steps e11 to e13:

[0363] In step e11 , the control device generates a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0364] Step e12: If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, the control device determines that the sample to be tested is a chylomicron sample; wherein, the sample with falsely high hemoglobin level includes a chylomicron sample.

[0365] Step e13: If the number of scattered particles included in the preset area of ​​the scattergram is less than a first preset number, the control device determines that the sample to be tested is not a chylous blood sample.

[0366] In other embodiments of the present application, step 404b may also be implemented by steps e14 to e17:

[0367] Step e14: Generate a scattergram based on the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information.

[0368] Step e15: Count the number of white blood cell particles in the scatter plot.

[0369] Step e16: If the number of white blood cell particles is greater than or equal to the second preset number, it is determined that the sample to be tested is a high white blood cell particle sample.

[0370] Among them, samples with falsely high hemoglobin levels include samples with high white blood cell particles.

[0371] Step e17: If the number of white blood cell particles is less than the second preset number, it is determined that the sample to be tested is not a high white blood cell particle sample.

[0372] Based on the above embodiment, in other embodiments of the present application, step 404 can also be implemented by steps 404c to 404g:

[0373] Step 404c: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value.

[0374] It should be noted that after the blood analyzer executes step 404c, it can choose to execute step 404d or steps 404e to 404g. If the mean corpuscular hemoglobin concentration is less than the preset threshold, the blood analyzer chooses to execute step 404d; if the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, the blood analyzer chooses to execute steps 404e to 404g:

[0375] Step 404d: If the mean corpuscular hemoglobin concentration is less than the preset threshold, the control device determines that the sample to be tested is not a chylous blood sample.

[0376] Step 404e: If the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, the control device calculates the difference between the first measurement value and the second measurement value.

[0377] Step 404f: If the difference is within the first preset range, the control device determines that the sample to be tested is not a chylous blood sample.

[0378] Step 404g: If the difference is within the second preset range, the control device determines that the sample to be tested is a chylous blood sample.

[0379] In other embodiments of the present application, before the blood analyzer executes step 404c, it may further execute steps 404h to 404i:

[0380] Step 404h: The control device controls the sample preparation device to mix part of the sample to be tested with the fourth reagent to prepare a fourth sample solution to be tested, and controls the sample preparation device to transport the fourth sample solution to be tested to the impedance detection device.

[0381] In the embodiment of the present application, the control device controls the sample preparation device to obtain a portion of the sample from the sample to be tested to obtain a fourth sample, and mixes the fourth sample with a fourth reagent to prepare a fourth sample liquid to be tested.

[0382] Step 404i: The control device controls the impedance detection device to perform red blood cell detection on the fourth sample solution using the impedance method to obtain the number of red blood cells and the mean red blood cell volume.

[0383] Correspondingly, step 404c can be specifically implemented by step f11:

[0384] Step f11: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, the number of red blood cells, and the mean corpuscular volume.

[0385] Based on the above embodiment, in other embodiments of the present application, step 404 can also be implemented by step 404j, and the blood analyzer further includes a communication device:

[0386] Step 404j: The control device controls the communication device to receive information sent by the external device indicating whether the sample to be processed is a sample with falsely high hemoglobin.

[0387] In the embodiment of the present application, the external device may be a sample analyzer or a medical management system. Correspondingly, the blood analyzer receives the test result sent by the sample analyzer or the medical management system to determine whether the sample to be tested is a sample with falsely high hemoglobin.

[0388] The test result is obtained by a sample analyzer to determine whether the sample to be tested is a sample with falsely high hemoglobin, or the test result is stored in a medical management system.

[0389] Correspondingly, step 404j can be specifically implemented by the following steps: the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device.

[0390] In other embodiments of the present application, in step 404j, the control device determines whether the sample to be tested is a sample with falsely high hemoglobin based on information from an external device. This can be achieved by the following steps:

[0391] The control device analyzes the blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the blood smear image, which is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer; the sample with falsely high hemoglobin level includes a chylous blood sample, and the external device includes a cell morphology analyzer;

[0392] Alternatively, the control device analyzes the test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, and the test tube appearance image is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes a camera system;

[0393] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the information of the external device includes the bilirubin test results, the bilirubin test results are obtained by testing a part of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes a high bilirubin sample, and the external device includes a biochemical instrument.

[0394] In other embodiments of the present application, the external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin based on information from the external device:

[0395] The control device analyzes the blood smear image, the test tube appearance image, or the blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein, a sample with falsely elevated hemoglobin includes a chylomicron sample; the blood smear image is a blood smear prepared by a cell morphology analyzer and photographed and sent to the medical management system; the test tube appearance image is a blood anticoagulant test tube containing a portion of the sample to be tested is photographed and sent to the medical management system by a camera system; and the blood lipid concentration test result is a biochemical instrument that detects a portion of the sample to be tested and sends the result to the medical management system;

[0396] Alternatively, the control device analyzes the bilirubin test results to determine whether the sample to be tested is a high bilirubin sample; wherein, the falsely high hemoglobin sample includes a high bilirubin sample, and the bilirubin test results are sent to the medical management system after a part of the sample to be tested is tested by a biochemical instrument.

[0397] It should be noted that, for the description of the same steps and contents in this embodiment as those in other embodiments, reference can be made to the description in other embodiments and will not be repeated here.

[0398] The blood analysis method provided in the embodiment of the present application first uses a colorimetric method to detect the hemoglobin of the sample to be tested, then automatically determines whether the sample to be tested is a sample with falsely high hemoglobin, and when the sample to be tested is not a sample with falsely high hemoglobin, directly outputs a first measurement value obtained by using the colorimetric method to detect the hemoglobin of the sample to be tested; when the sample to be tested is a sample with falsely high hemoglobin, the light scattering method is used to detect the hemoglobin of the sample to be tested, and the corresponding test result is output, which solves the current problem of low efficiency of hemoglobin testing due to the need for manual judgment of whether the sample to be tested is a sample with falsely high hemoglobin, and realizes the use of a colorimetric method to detect the sample to be tested. After the hemoglobin is detected, if the automated detection sample is not a sample with falsely high hemoglobin, the test result of the colorimetric method is directly output, thereby improving the output efficiency of the test result; if the automated detection sample is a sample with falsely high hemoglobin, the light scattering method is continued to be used to detect the hemoglobin of the sample. Since the hemoglobin concentration result in the sample with falsely high hemoglobin detected by the light scattering method is more accurate than the hemoglobin concentration detected by the colorimetric method, the accuracy of the hemoglobin concentration test result is effectively guaranteed when the sample to be tested is a sample with falsely high hemoglobin, thereby effectively improving the reliability of the hemoglobin concentration test result of the blood analyzer.

[0399] It should be noted that, for the description of the same steps and the same contents in any of the aforementioned blood analyzers and any of the blood analysis methods, reference can be made to the description in other embodiments, and no repetition will be given.

[0400] It should be understood that the features, structures and advantages mentioned in the specification, claims and drawings can be arbitrarily combined with each other as long as they are meaningful within the scope of this application. The features, structures and advantages described for the method of the present application are applicable to the blood analyzer of the present invention in a corresponding manner, and vice versa. It should be understood that in the various embodiments of the present application, the size of the sequence number of each process does not mean the order of execution, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiment of the present application. The above-mentioned sequence numbers of the embodiments of the present application are only for description and do not represent the pros and cons of the embodiments.

[0401] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus comprising the element.

[0402] In the several embodiments provided in this application, it should be understood that the disclosed devices and methods can be implemented in other ways. The device embodiments described above are merely schematic. For example, the division of units is merely a logical function division. In actual implementation, there may be other division methods, such as: multiple units or components can be combined, or can be integrated into another system, or some features can be ignored or not executed. In addition, the coupling, direct coupling, or communication connection between the components shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical, mechanical or other forms.

[0403] The units described above as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units; they may be located in one place or distributed across multiple network units; some or all of the units may be selected according to actual needs to achieve the purpose of the scheme of this embodiment.

[0404] In addition, all functional units in the embodiments of the present application can be integrated into one processing unit, or each unit can be a separate unit, or two or more units can be integrated into one unit; the above-mentioned integrated units can be implemented in the form of hardware or in the form of hardware plus software functional units.

[0405] The above is merely an embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A blood analyzer, characterized in that: The blood analyzer comprises: A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested; The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested; a colorimetric detection device for performing a hemoglobin test on a first sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first sample solution is prepared by combining a portion of the sample solution and a first reagent; an optical detection device for performing a hemoglobin detection on a second sample solution prepared by the sample preparation device using a light scattering method to obtain a second measurement value, wherein the second sample solution is prepared by a portion of the sample solution and a second reagent; a processor electrically connected to the optical detection device and the colorimetric detection device, and configured to: Obtaining the first measurement value and the second measurement value from the colorimetric detection device and the optical detection device; Determining whether the sample to be tested is a sample with falsely high hemoglobin; If the sample to be tested is the sample with falsely high hemoglobin, outputting the second measurement value; If the sample to be tested is not the sample with falsely high hemoglobin, the first measurement value is output.

2. The blood analyzer according to claim 1, characterized in that The optical detection device is further used to perform white blood cell detection on the third sample solution prepared by the sample preparation device using a light scattering method, wherein the third sample solution is prepared by a portion of the sample solution and a third reagent; The processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin: Acquiring first scattered light information detected when performing white blood cell detection on the third sample liquid from the optical detection device; It is determined whether the sample to be tested is the sample with falsely high hemoglobin according to the first scattered light information.

3. The blood analyzer according to claim 2, characterized in that The processor is configured to perform the following steps when determining whether the sample to be tested is the sample with falsely high hemoglobin according to the first scattered light information: generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information; If the number of scattered particles included in the preset area of ​​the scatter plot is greater than or equal to a first preset number, it is determined that the sample to be tested is a chylomicron sample; wherein the sample with falsely high hemoglobin level includes the chylomicron sample; If the number of scattered particles included in the preset area of ​​the scattergram is less than the first preset number, it is determined that the sample to be tested is not a chylous blood sample.

4. The blood analyzer according to claim 1, characterized in that The processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin: Calculating the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value; If the mean corpuscular hemoglobin concentration is less than a preset threshold, it is determined that the sample to be tested is not a chylomicron sample; wherein the sample with falsely high hemoglobin includes the chylomicron sample.

5. The blood analyzer according to claim 4, characterized in that The blood analyzer further includes an impedance detection device for performing a red blood cell test on a fourth sample solution prepared by the sample preparation device using an impedance method to obtain the number of red blood cells and the mean red blood cell volume, wherein the fourth sample solution is prepared by a portion of the sample solution and a fourth reagent; The processor is configured to perform the following steps when calculating the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value: The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

6. The blood analyzer according to claim 4, characterized in that The processor is configured to perform the following steps when calculating the mean blood cell hemoglobin concentration in the sample to be tested based on the first measurement value: Acquiring, from the optical detection device, second scattered light information detected when performing hemoglobin detection on the second sample liquid to be tested; determining the number of red blood cells and the average volume of red blood cells in the sample to be tested based on the second scattered light information; The mean corpuscular hemoglobin concentration of the sample to be tested is calculated according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

7. The blood analyzer according to any one of claims 4 to 6, characterized in that: The processor is further configured to perform the following steps: If the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, calculating the difference between the first measurement value and the second measurement value; If the difference is within a first preset range, it is determined that the sample to be tested is not a chylous blood sample; If the difference is not within the first preset range, it is determined that the sample to be tested is a chylous blood sample.

8. The blood analyzer according to claim 1, characterized in that The processor is configured to further perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin: Acquiring a blood smear image through a cell morphology analyzer, and analyzing the blood smear image to determine whether the sample to be tested is a chylomicronized blood sample; wherein the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested through the cell morphology analyzer, and the sample with falsely high hemoglobin level includes the chylomicronized blood sample; Alternatively, a test tube appearance image is acquired by a camera system, and the test tube appearance image is analyzed to determine whether the sample to be tested is the chylous blood sample; wherein the test tube appearance image is obtained by the camera system photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested, and the blood analyzer includes the camera system; Alternatively, a bilirubin test result is obtained by a biochemical instrument, and the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the bilirubin test result is obtained by testing a portion of the sample to be tested by the biochemical instrument, and the falsely high hemoglobin sample includes the high bilirubin sample; Alternatively, the blood lipid concentration test result is obtained by the biochemical instrument, and the blood lipid concentration test result is analyzed to determine whether the sample to be tested is the chylosing blood sample; wherein the blood lipid concentration test result is obtained by testing a part of the sample to be tested by the biochemical instrument.

9. The blood analyzer according to claim 1, characterized in that The blood analyzer further includes a communication device for communicating with an external device so as to obtain information related to whether the sample to be tested is a sample with falsely high hemoglobin from the external device; The processor is configured to perform the following steps when determining whether the sample to be tested is a sample with falsely high hemoglobin: Based on the information from the external device, it is determined whether the sample to be tested is the sample with falsely high hemoglobin.

10. The blood analyzer according to claim 9, characterized in that: The processor is configured to perform the following steps when determining whether the sample to be tested is the sample with falsely high hemoglobin based on the information from the external device: Analyzing a blood smear image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the blood smear image, the blood smear image is obtained by photographing a blood smear prepared from a portion of the sample to be tested using a cell morphology analyzer, the sample with falsely high hemoglobin levels includes the chylous blood sample, and the external device includes the cell morphology analyzer; Alternatively, analyzing a test tube appearance image to determine whether the sample to be tested is a chylous blood sample; wherein the information of the external device includes the test tube appearance image, and the test tube appearance image is obtained by photographing the appearance of a blood anticoagulation test tube containing a portion of the sample to be tested by a camera system, and the external device includes the camera system; Alternatively, the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the information of the external device includes the bilirubin test result, the bilirubin test result is obtained by testing a portion of the sample to be tested by a biochemical instrument, the falsely high hemoglobin sample includes the high bilirubin sample, and the external device includes the biochemical instrument.

11. The blood analyzer according to claim 9, characterized in that The external device includes a medical management system, and the processor is configured to perform the following steps when determining whether the sample to be tested is the sample with falsely high hemoglobin based on information from the external device: Analyzing a blood smear image, a test tube appearance image, or a blood lipid concentration test result to determine whether the sample to be tested is a chylomicron sample; wherein the sample with falsely elevated hemoglobin includes the chylomicron sample; the blood smear image is a blood smear prepared by a cell morphology analyzer photographing a portion of the sample to be tested and then sent to the medical management system; the test tube appearance image is a blood anticoagulation test tube containing a portion of the sample to be tested photographed by a camera system and then sent to the medical management system; and the blood lipid concentration test result is a biochemical instrument testing a portion of the sample to be tested and then sent to the medical management system; Alternatively, the bilirubin test result is analyzed to determine whether the sample to be tested is a high bilirubin sample; wherein the falsely high hemoglobin sample includes the high bilirubin sample, and the bilirubin test result is sent to the medical management system after the biochemical instrument tests a part of the sample to be tested.

12. The blood analyzer according to claim 2, characterized in that: The processor is configured to further perform the following steps when determining whether the sample to be tested is the sample with falsely high hemoglobin according to the first scattered light information: generating a scatter plot according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information; Counting the number of leukocyte particles in the scattergram; If the number of white blood cell particles is greater than or equal to a second preset number, the sample to be tested is determined to be a high white blood cell particle sample; wherein the falsely high hemoglobin sample includes the high white blood cell particle sample; If the number of white blood cell particles is less than the second preset number, it is determined that the sample to be tested is not the high white blood cell particle sample.

13. A blood analyzer, characterized in that: The blood analyzer comprises: A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested; The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested; A colorimetric detection device, used for detecting hemoglobin in the sample to be tested using a colorimetric method; An optical detection device for detecting hemoglobin in the sample to be tested using a light scattering method; a processor electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and configured to: Determining whether the sample to be tested is a sample with falsely high hemoglobin; If the sample to be tested is not a sample with falsely high hemoglobin, controlling the sample preparation device to prepare a portion of the sample to be tested with a first reagent to obtain a first sample solution to be tested, and controlling the colorimetric detection device to perform a hemoglobin test on the first sample solution to obtain a first measurement value; If the sample to be tested is a sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample solution to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample solution to obtain a second measurement value.

14. A blood analyzer, characterized in that: The blood analyzer comprises: A sampling device comprising a pipette and a driving unit for driving the pipette to suck a sample to be tested; The sample preparation device comprises a reaction pool and a reagent supply portion, wherein the reaction pool is used to receive a portion of the sample to be tested sucked by the sampling device, and the reagent supply portion provides a processing reagent to the reaction pool, so that the portion of the sample to be tested sucked by the sampling device and the processing reagent provided by the reagent supply portion are mixed in the reaction pool to prepare a sample liquid to be tested; a colorimetric detection device for performing a hemoglobin test on a first sample solution prepared by the sample preparation device using a colorimetric method to obtain a first measurement value, wherein the first sample solution is prepared by combining a portion of the sample solution and a first reagent; An optical detection device for detecting hemoglobin in the sample to be tested using a light scattering method; a processor electrically connected to the sample preparation device, the optical detection device, and the colorimetric detection device, and configured to: Determining whether the sample to be tested is a sample with falsely high hemoglobin; If the sample to be tested is not a sample with falsely high hemoglobin, outputting the first measurement value; If the sample to be tested is a sample with falsely high hemoglobin, the sample preparation device is controlled to prepare a portion of the sample to be tested with a second reagent to obtain a second sample liquid to be tested, and the optical detection device is controlled to perform hemoglobin detection on the second sample liquid to be tested to obtain a second measurement value, and the second measurement value is output.

15. A hemoglobin detection method, characterized in that: The method is applied to a blood analyzer, and the method comprises: The control device controls the sampling device to obtain a sample to be tested; the control device controls the sample preparation device to mix a portion of the sample to be tested with a first reagent to prepare a first sample liquid to be tested, and to mix a portion of the sample to be tested with a second reagent to prepare a second sample liquid to be tested; The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample liquid to be tested using the colorimetric method to obtain a first measurement value; The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid to be tested by using a light scattering method to obtain a second measurement value; The control device determines whether the sample to be tested is a sample with falsely high hemoglobin levels. If the sample to be tested is a sample with falsely high hemoglobin levels, the control device outputs the second measurement value. If the sample to be tested is not a sample with falsely high hemoglobin levels, the control device outputs the first measurement value.

16. The method according to claim 15, characterized in that The method further comprises: The control device controls the sample preparation device to mix a portion of the sample to be tested with a third reagent to prepare a third sample liquid to be tested; The control device controls the sample preparation device to transport the third sample liquid to be tested to the optical detection device, and performs white blood cell detection on the third sample liquid to be tested using a light scattering method; Correspondingly, the control device determines whether the sample to be tested is a sample with falsely high hemoglobin, including: The control device obtains first scattered light information detected when performing white blood cell detection on the third sample liquid to be tested; The control device determines whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information.

17. The method according to claim 16, characterized in that The control device determines whether the sample to be tested is a sample with falsely high hemoglobin according to the first scattered light information, including: The control device generates a scattergram according to the forward scattered light intensity information and the side scattered light intensity information in the first scattered light information; If the number of scattered particles included in the preset area of ​​the scattergram is greater than or equal to a first preset number, the control device determines that the sample to be tested is a chylomicron sample; wherein the sample with falsely high hemoglobin level includes the chylomicron sample; If the number of scattered particles included in the preset area of ​​the scattergram is less than the first preset number, the control device determines that the sample to be tested is not a chylous blood sample.

18. The method according to claim 15, characterized in that The control device determines whether the sample to be tested is the sample with falsely high hemoglobin, including: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value; If the mean corpuscular hemoglobin concentration is less than a preset threshold, the control device determines that the sample to be tested is not a chylomicron sample; wherein the sample with falsely high hemoglobin includes the chylomicron sample.

19. The method according to claim 18, characterized in that The method further comprises: The control device controls the sample preparation device to mix a portion of the sample to be tested with the fourth reagent to prepare a fourth sample liquid to be tested; The control device controls the sample preparation device to transport the fourth sample liquid to be tested to the impedance detection device, and performs red blood cell detection on the fourth sample liquid to be tested using the impedance method to obtain the red blood cell count and the mean red blood cell volume; Correspondingly, the control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, including: The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

20. The method according to claim 18, wherein The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested based on the first measurement value, further comprising: The control device obtains second scattered light information detected when the hemoglobin test is performed on the second sample liquid to be tested; The control device determines the number of red blood cells and the average volume of red blood cells in the sample to be tested based on the second scattered light information; The control device calculates the mean corpuscular hemoglobin concentration of the sample to be tested according to the first measurement value, the number of red blood cells and the mean corpuscular volume.

21. The method according to any one of claims 18 to 20, characterized in that The method further comprises: If the mean corpuscular hemoglobin concentration is greater than or equal to the preset threshold, the control device calculates the difference between the first measurement value and the second measurement value; If the difference is within a first preset range, the control device determines that the sample to be tested is not a chylous blood sample; If the difference is within a second preset range, the control device determines that the sample to be tested is a chylous blood sample.

22. A hemoglobin detection method, characterized in that: The method is applied to a blood analyzer, and the method comprises: The control device controls the sampling device to obtain the sample to be tested; The control device determines whether the sample to be tested is a sample with falsely high hemoglobin levels. If the sample to be tested is not a sample with falsely high hemoglobin levels, the control device controls the sample preparation device to mix a portion of the sample to be tested with a first reagent to prepare a first sample solution to be tested. The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample liquid to be tested using the colorimetric method to obtain a first measurement value; If the sample to be tested is the sample with falsely high hemoglobin, the control device controls the sample preparation device to mix part of the sample to be tested with the second reagent to prepare a second sample solution to be tested; The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid to be tested by using a light scattering method to obtain a second measurement value.

23. A hemoglobin detection method, characterized in that: The method is applied to a blood analyzer, and the method comprises: The control device controls the sampling device to obtain the sample to be tested; the control device controls the sample preparation device to mix part of the sample to be tested with the first reagent to prepare a first sample liquid to be tested; The control device controls the colorimetric detection device to perform hemoglobin detection on the first sample liquid to be tested using the colorimetric method to obtain a first measurement value; The control device determines whether the sample to be tested is a sample with falsely high hemoglobin; If the sample to be tested is not the sample with falsely high hemoglobin, the control device outputs the first measurement value; If the sample to be tested is the sample with falsely high hemoglobin, the control device controls the sample preparation device to mix part of the sample to be tested with the second reagent to prepare a second sample solution to be tested; The control device controls the optical detection device to perform hemoglobin detection on the second sample liquid to be tested by using a light scattering method to obtain a second measurement value, and outputs the second measurement value.

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