A needle breaking device for a needle for microinjection of caenorhabditis elegans

Abstract

The application discloses a needle breaking device for microinjection of Caenorhabditis elegans, and relates to the technical field of microinjection, which comprises a fixed needle breaking mechanism and an automatic needle breaking mechanism matched with the fixed needle breaking mechanism; the fixed needle breaking mechanism is provided with a fixed needle control lower disc and a rotating needle control cover body matched with the fixed needle control lower disc; the fixed needle control lower disc is provided with a plurality of radial needle control grooves with equal radian and used for mounting microinjection needles to be broken; and a corresponding end of the radial needle control grooves penetrates through one side of the outer periphery of the fixed needle control lower disc to allow the microinjection needles to be broken to pass out; the rotating needle control cover body is provided with a driving element used for driving the microinjection needles to be broken mounted in the radial needle control grooves to pass out from one side of the outer periphery of the fixed needle control lower disc at a length. The application has the effect of significantly improving the consistency of the breaking position and angle.

Description

Technical Field

[0001] This application relates to the field of microinjection technology, and in particular to a needle-breaking device for microinjection of Caenorhabditis elegans. Background Technology

[0002] Gene editing, also known as genome editing or genome engineering, is a relatively new and precise genetic engineering technology or process that can modify specific target genes in an organism's genome. Early genetic engineering techniques could only randomly insert exogenous or endogenous genetic material into the host genome, while gene editing can precisely edit the desired gene. Gene editing relies on genetically engineered nucleases, also known as "molecular scissors," to create site-specific double-strand breaks (DSBs) at specific locations in the genome. This induces the organism to repair the DSBs through non-homologous end joining (NHEJ) or homologous recombination (HR). Because this repair process is prone to errors, it can lead to targeted mutations. These targeted mutations constitute gene editing. Gene microinjection is a method of directly injecting recombinant DNA into the cytoplasm or nucleus of mammalian cells using a microinjector. It is one of the main methods for introducing genes into mammalian cells in transgenic animal technology.

[0003] Chinese Patent No. CN208151348U discloses a simple needle breaking device for chicken embryo microinjection. The simple needle breaking device for chicken embryo microinjection includes a battery pack, a controller, a motor, a coupling, and a grinding wheel. The battery pack is connected to the motor through the controller, the motor is connected to the battery pack through a wire, and the motor drives the grinding wheel through the coupling.

[0004] However, this simple needle-breaking device for chicken embryo microinjection directly grinds the unfixed glass needle with a grinding wheel during use. As a result, the glass needle will move relative to the ground needle due to the external force of the grinding wheel during the needle breaking process, making it difficult to ensure the consistency of the broken needle location and angle. This needs to be improved. Summary of the Invention

[0005] In view of this, the purpose of this application is to provide a needle-breaking device for a microinjection needle for Caenorhabditis elegans, so as to significantly improve the consistency of the needle-breaking site and angle. The specific solution is as follows: A needle-breaking device for microinjection of *Caenorhabditis elegans* includes a fixed needle-breaking mechanism and an automatic needle-breaking mechanism matched with the fixed needle-breaking mechanism. The fixed needle-breaking mechanism is provided with a fixed needle-control plate and a rotating needle-control cover matched with the fixed needle-control plate. The fixed needle-control plate is provided with a plurality of radially distributed, equally arcuate grooves for mounting microinjection needles to be broken, and one end of each radially distributed groove penetrates one side of the outer circumference of the fixed needle-control plate to allow the microinjection needles to be broken to pass through. The rotating needle-control cover is provided with a driving member for driving the microinjection needles to be broken, mounted in the plurality of radially distributed grooves, to pass through the outer circumference of the fixed needle-control plate by a certain length.

[0006] Preferably, the driving component includes a drive needle control motor and a pusher detachably connected to the rotating needle control cover; the drive needle control motor is used to drive the rotating needle control cover to perform circumferential reciprocating motion; the lower side of the rotating needle control cover is provided with a plurality of spiral transition grooves respectively for matching the corresponding radial needle control grooves; the pusher includes a spiral post with both ends inserted into the spiral transition grooves and a push plate inserted into the radial needle control grooves, and a connecting post connecting the spiral post and the push plate; a corresponding side of the push plate is used to abut against a corresponding end of the microinjection needle to be punctured.

[0007] Preferably: the upper end of the spiral insert is provided with an upper through hole, the connecting post is vertically inserted with a lifting locking post, and is provided with a side through groove located on the outside; the upper end of the lifting locking post extends out from the upper through hole, the lower end abuts against a return spring inserted in the connecting post, and the lower front end is provided with an arc-shaped abutment elastic part extending out from the side through groove.

[0008] Preferably, a rubber sleeve is inserted into the radial needle control groove, and an injection needle slot and an upper opening located on the upper side for the push plate to move into the injection needle slot are formed in the rubber sleeve; the upper side of the rubber sleeve is provided with a contact upper surface that contacts the lower side of the rotating needle control cover.

[0009] Preferably, the lower side of the fixed needle breaking mechanism is provided with a vertical needle feeding mechanism for driving the fixed needle breaking mechanism to move to match the automatic needle breaking mechanism; the vertical needle feeding mechanism includes a support plate that is fixedly connected to the fixed control needle lower plate; a motor frame is provided on the lower side of the support plate, the drive control needle motor is fixed in the motor frame, and the output end is sleeved with a fixed needle breaking bearing located in the fixed control needle lower plate; the axes of the drive control needle motor, the fixed needle breaking bearing, the fixed control needle lower plate and the rotating control needle cover are coincident, and the motor frame is provided with heat dissipation holes.

[0010] Preferably, the vertical needle feeding mechanism further includes a rotating insertion part, an external gear, and a threaded rod connected sequentially from top to bottom; the rotating insertion part is sleeved with a vertical needle feeding bearing located at the bottom of the motor frame; the external gear is meshed with spur teeth for driving rotation; and the threaded rod is threadedly connected to a threaded connecting block.

[0011] Preferably, the threaded connecting block is connected to a needle feed telescopic device for horizontal driving movement and a needle breaking base; the straight teeth are arranged horizontally and are fixedly connected to a fixing rod that is fixedly connected to the needle breaking base.

[0012] Preferably: the needle-breaking base is provided with a convex groove along its length, and the bottom of the threaded connecting block matches the convex groove; the threaded connecting block is fixedly connected to a matching vertical plate, and the support plate is provided with an abutting surface for abutting against the matching vertical plate.

[0013] Preferably, the needle-breaking base is provided with a support column for connecting and fixing the automatic needle-breaking mechanism; the automatic needle-breaking mechanism includes a dust cover on the lower side and a vacuum cleaner and a rotary motor on the upper side. The dust cover has a matching window at the end facing the fixed needle-breaking mechanism. The vacuum cleaner is connected to multiple suction pipes with equal arc distribution. The output end of the rotary motor is connected to a rotating rod. The rotating rod is connected to a grinding cutter. The rotary motor is used to drive the grinding cutter to rotate circumferentially along one side of the outer circumference of the lower plate of the fixed control needle.

[0014] Preferably, the matching vertical plate is provided with a window fitting portion that matches the matching window and a matching slot through which the straight teeth pass.

[0015] As can be seen from the above solutions, this application provides a needle-breaking device for a microinjection needle for Caenorhabditis elegans, which has the following beneficial effects: 1. Multiple microinjection needles to be broken are fixed by a fixed needle breaking mechanism, and then the needle breaking process is carried out on the part of the multiple microinjection needles to be broken through the fixed needle control plate, thereby achieving the purpose of improving the consistency of the needle breaking location and angle. 2. By driving the control needle motor to rotate the control needle cover circumferentially, the spiral insert located in the spiral transition groove moves along the spiral transition groove, while the push plate moves in the radial control needle groove, thereby achieving the effect of driving multiple micro-injection needles to be broken to simultaneously pass out of the fixed control needle lower plate and maintaining the same passing length. 3. By rotating the control needle cover and fixing the lower control needle plate to close, the rotating control needle cover presses the lifting locking column, and the arc-shaped abutment elastic part on the lifting locking column fixes the corresponding micro-injection needle to be broken, thereby maintaining the push plate and the corresponding micro-injection needle to be broken at a relatively stationary position, so as to improve the consistency of the needle breaking position and angle. 4. By cooperating with the vertical needle feeding mechanism and the needle feeding telescopic device, the dust cover and the window fitting part are matched. At the same time, the grinding and cutting material is collected to avoid environmental pollution and affect human health. 5. By replacing the specifications and models of the rubber sleeve, different injection needle slots can be matched with the corresponding micro-injection needles to be ruptured, thereby completing the rupture process of the corresponding micro-injection needles to be ruptured, significantly improving the practicality and effectiveness of the rupture device for the Caenorhabditis elegans micro-injection needle. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this application. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the open state structure of the needle breaking device of the microinjection needle for Caenorhabditis elegans disclosed in this application; Figure 2 This is a schematic diagram of the closed-state structure of the needle breaking device of the microinjection needle for Caenorhabditis elegans disclosed in this application; Figure 3 This is an exploded structural diagram of the combination of the fixed needle breaking mechanism and the vertical needle feeding mechanism disclosed in this application; Figure 4 for Figure 3 A magnified structural diagram of part A in the middle; Figure 5 This is a schematic diagram of the rotating control needle cover disclosed in this application.

[0018] Explanation of reference numerals in the attached drawings: 1. Fixed needle breaking mechanism; 11. Fixed needle control plate; 111. Radial needle control groove; 12. Rotating needle control cover; 121. Drive needle control motor; 122. Spiral adapter groove; 13. Rubber sleeve; 131. Injection needle slot; 1311. Upper opening; 132. Contact upper plane; 133. Push plate; 134. Spiral insertion post; 1341. Upper through hole; 135. Connecting post; 1351. Side through groove; 136. Lifting locking post; 1361. Arc-shaped abutment elastic part; 137. Return spring; 14. Fixed needle breaking bearing; 2. Vertical needle feeding mechanism; 21. External gear. 22. Threaded rod; 23. Rotating insertion part; 24. Support plate; 241. Motor frame; 242. Heat dissipation hole; 243. Abutting surface; 25. Vertical needle feed bearing; 3. Needle feed telescopic device; 31. Threaded connecting block; 32. Matching vertical plate; 321. Window fitting part; 322. Matching slot; 4. Needle breaking base; 41. Convex groove; 42. Support column; 43. Straight tooth; 431. Fixing rod; 5. Automatic needle breaking mechanism; 51. Dust cover; 511. Matching window; 52. Vacuum cleaner; 521. Vacuum suction pipe; 53. Rotating motor; 531. Rotating rod; 532. Grinding and cutting tool. Implementation

[0019] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0020] like Figure 1 As shown, a needle breaking device for a microinjection needle for Caenorhabditis elegans includes a fixed needle breaking mechanism 1 and an automatic needle breaking mechanism 5 matched with the fixed needle breaking mechanism 1.

[0021] The fixed needle breaking mechanism 1 includes a fixed needle control plate 11 and a rotating needle control cover 12 that matches the fixed needle control plate 11. Figure 3Multiple radial needle control grooves 111, evenly distributed, are provided within the fixed needle control plate 11 for mounting microinjection needles to be punctured. The upper side of each radial needle control groove 111 has an opening formed on the fixed needle control plate 11, which matches the rotating needle control cover 12 to control the microinjection needle to be punctured. When combined with the automatic needle puncture mechanism 5, the puncture operation of the microinjection needle to be punctured is completed, improving the consistency of the puncture site and angle. Simultaneously, one end of each radial needle control groove 111 penetrates one side of the outer circumference of the fixed needle control plate 11, allowing the microinjection needle to be punctured to exit. The automatic needle puncture mechanism 5 then completes the puncture operation of the end of the microinjection needle to be punctured that exits from the outer circumference of the fixed needle control plate 11.

[0022] It should be mentioned that the rotating needle control cover 12 is provided with a drive component. The drive component is used to drive the microinjection needles of the needles to be punctured, which are installed in multiple radial needle control slots 111, to extend out of the outer side of the fixed needle control plate 11 by a certain length.

[0023] Among them, such as Figure 3 , Figure 4 , Figure 5 As shown, the driving component includes a drive needle control motor 121 detachably connected to the rotating needle control cover 12 and a pusher. The output shaft of the drive needle control motor 121 is aligned with the axis of the rotating needle control cover 12 to drive the rotating needle control cover 12 to reciprocate in the circumferential direction. Multiple helical adapter grooves 122 are provided on the lower side of the rotating needle control cover 12, each designed to match a corresponding radial needle control groove 111.

[0024] Correspondingly, the pusher includes a spiral insert 134 inserted into the spiral transition groove 122 at both ends, a push plate 133 inserted into the radial control needle groove 111, and a connecting post 135 connecting the spiral insert 134 and the push plate 133. A corresponding side of the push plate 133 is used to abut against a corresponding end of the microinjection needle to be punctured. Therefore, when the drive needle control motor 121 drives the rotating needle control cover 12 to rotate circumferentially, the spiral insert 134 will move along the spiral transition groove 122. The spiral transition groove 122 adopts an Archimedean spiral, which will cause the push plate 133 to move radially along the radial needle control groove 111. During the movement, the push plate 133 will push the corresponding micro-injection needle to be broken out from the outer peripheral side wall of the fixed needle control plate 11, thereby effectively controlling the needle length and angle of the micro-injection needle to be broken out. This, combined with the automatic needle breaking mechanism 5, completes the needle breaking treatment of the corresponding micro-injection needle to be broken out with a high degree of consistency in the breaking position and angle.

[0025] It should be mentioned that, in order to further improve the fixation stability of multiple microinjection needles to be punctured, thereby achieving the effect of improving the consistency of the puncture site and angle, an upper through hole 1341 is provided at the upper end of the spiral insert 134, and a lifting locking pin 136 is inserted into the connecting pin 135 in the vertical direction, and a side through groove 1351 is provided on the outside of the connecting pin 135. The upper end of the lifting locking pin 136 extends out of the upper through hole 1341, and the lower end abuts against a return spring 137 inserted in the connecting pin 135. An arc-shaped abutment elastic part 1361 extending out of the side through groove 1351 is provided at the lower front side of the lifting locking pin 136.

[0026] The side through-groove 1351 faces the corresponding radial control needle groove 111, so as to cooperate with the arc-shaped abutment elastic part 1361 extending from the side through-groove 1351 to abut and fix the corresponding micro-injection needle to be punctured. Furthermore, since the upper end of the lifting locking pin 136 extends from the upper through-hole 1341 when the return spring 137 is at its maximum length, when the rotating control needle cover 12 covers the upper side of the fixed control needle lower plate 11, the lower side of the rotating control needle cover 12 will first abut against the top of the lifting locking pin 136, causing the lifting locking pin 136 to apply pressure to the return spring 137. After the return spring 137 is compressed and deformed, the arc-shaped abutment elastic part 1361 abuts and fixes the micro-injection needle to be punctured, thereby significantly improving the fixation stability of multiple micro-injection needles to be punctured.

[0027] To further enhance the practicality and effectiveness of the needle-breaking device for the *C. elegans* microinjection needle, a rubber sleeve 13 is inserted into the radial needle control groove 111. The rubber sleeve 13 contains an injection needle slot 131 and an upper opening 1311 located on its upper side for the push plate 133 to move within the injection needle slot 131. Simultaneously, a contact surface 132 is provided on the upper side of the rubber sleeve 13, contacting the lower side of the rotating needle control cover 12. Therefore, by replacing the specifications of the rubber sleeve 13, different injection needle slots 131 can be matched with corresponding microinjection needles to be broken, thereby completing the needle-breaking process of the corresponding microinjection needles to be broken, significantly improving the practicality and effectiveness of the *C. elegans* microinjection needle needle-breaking device.

[0028] like Figure 1 , Figure 2 As shown, a vertical needle feeding mechanism 2 is provided on the lower side of the fixed needle breaking mechanism 1 to drive the fixed needle breaking mechanism 1 to move to match the automatic needle breaking mechanism 5.

[0029] The vertical needle feeding mechanism 2 includes a support plate 24 fixedly connected to the fixed needle control plate 11. A motor frame 241 is provided on the lower side of the support plate 24. The drive needle control motor 121 is fixed inside the motor frame 241, and its output end is sleeved with a fixed needle breaking bearing 14 located inside the fixed needle control plate 11. It should be noted that the axes of the drive needle control motor 121, the fixed needle breaking bearing 14, the fixed needle control plate 11, and the rotating needle control cover 12 are coincident, thereby achieving stable driving. Furthermore, heat dissipation holes 242 are provided on the motor frame 241 to achieve effective heat dissipation.

[0030] Meanwhile, the vertical needle feeding mechanism 2 also includes a rotating insertion part 23, an external gear 21, and a threaded rod 22 connected sequentially from top to bottom. The rotating insertion part 23 is fitted with a vertical needle feeding bearing 25 located at the bottom of the motor frame 241; the external gear 21 meshes with a spur gear 43 for driving rotation; and the threaded rod 22 is threadedly connected to a threaded connecting block 31. The threaded connecting block 31 is connected to a needle feeding telescoping device 3 for horizontal driving movement and a needle breaking base 4; the spur gear 43 is arranged horizontally and is fixedly connected to a fixing rod 431 that is fixedly connected to and fixed to the needle breaking base 4.

[0031] like Figure 1 , Figure 2 As shown, the needle-breaking base 4 has a convex groove 41 along its length. The bottom of the threaded connecting block 31 matches the convex groove 41, so that the threaded connecting block 31 maintains a stable moving path and posture under the constraint of the convex groove 41 when making horizontal linear movement. A matching vertical plate 32 is fixedly connected to one end of the threaded connecting block 31, and the support plate 24 is provided with an abutting surface 243 for abutting against the matching vertical plate 32. Thus, when the rotating insertion part 23, the external gear 21 and the threaded rod 22 make circumferential rotation, they only play the role of lifting the support plate 24, while the support plate 24 maintains a vertical lifting movement relative to the matching vertical plate 32 by abutting against the matching vertical plate 32 through the abutting surface 243.

[0032] It should be mentioned that the needle-breaking base 4 is provided with a support column 42 for connecting and fixing the automatic needle-breaking mechanism 5. The automatic needle-breaking mechanism 5 includes a dust cover 51 located on the lower side and a vacuum cleaner 52 and a rotating motor 53 located on the upper side. A matching window 511 is provided at the end of the dust cover 51 facing the fixed needle-breaking mechanism 1.

[0033] The vacuum cleaner 52 is connected to multiple suction pipes 521 with equal arc distribution. The output end of the rotating motor 53 is connected to a rotating rod 531, which is connected to a grinding cutter 532. The rotating motor 53 drives the grinding cutter 532 to rotate circumferentially along one side of the outer circumference of the fixed needle control plate 11. The matching vertical plate 32 is provided with a window fitting part 321 that matches the matching window 511 and a matching slot 322 through which the straight teeth 43 pass. Therefore, the cooperation between the vertical needle feeding mechanism 2 and the needle feeding telescopic device 3 achieves the cooperation between the dust cover 51 and the window fitting part 321. This allows for the consistent needle breaking position and angle of multiple micro-injection needles to be broken by the grinding cutter 532, while simultaneously collecting the ground material to avoid environmental pollution and harm to health.

[0034] In summary, this application provides a needle-breaking device for microinjection needles of *C. elegans*. This device fixes multiple microinjection needles to be broken by a needle-breaking mechanism 1, and then breaks the needles at the points where they emerge from the fixed needle control plate 11, thereby improving the consistency of the breaking point and angle. Simultaneously, the needle control motor 121 drives the circumferential rotation of the rotating needle control cover 12, which in turn drives the spiral insertion post 134 located in the spiral transition groove 122 to move along the spiral transition groove 122, while simultaneously driving the push plate. 133 moves within the radial needle control groove 111, thereby driving multiple micro-injection needles to be punctured to simultaneously extend outwards towards the fixed needle control plate 11 while maintaining a consistent extension length. Additionally, by rotating the needle control cover 12 and matching it with the fixed needle control plate 11, the rotating needle control cover 12 presses down the lifting locking pin 136, causing the arc-shaped abutment elastic part 1361 on the lifting locking pin 136 to fix the corresponding micro-injection needles to be punctured. This maintains the push plate 133 and the corresponding micro-injection needles to be punctured at a relatively stationary position, thereby improving the consistency of the puncture site and angle.

[0035] The terms “first,” “second,” “third,” “fourth,” etc., used in this application (if applicable) are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms “comprising” and “having,” and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, or apparatus that includes a series of steps or units is not necessarily limited to those explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, or apparatus.

[0036] It should be noted that the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of those features. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed in this application.

[0037] This document uses specific examples to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the methods and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A needle-breaking device for microinjection of Caenorhabditis elegans, characterized in that: The device includes a fixed needle breaking mechanism (1) and an automatic needle breaking mechanism (5) matched with the fixed needle breaking mechanism (1); the fixed needle breaking mechanism (1) is provided with a fixed needle control plate (11) and a rotating needle control cover (12) matched with the fixed needle control plate (11); the fixed needle control plate (11) is provided with a plurality of radial needle control grooves (111) with equal arc distribution for installing micro-injection needles to be broken, and one end of each radial needle control groove (111) penetrates one side of the outer circumference of the fixed needle control plate (11) for the micro-injection needles to be broken to pass through; the rotating needle control cover (12) is provided with a driving member, which is used to drive the micro-injection needles to be broken installed in the plurality of radial needle control grooves (111) to pass through a certain length from one side of the outer circumference of the fixed needle control plate (11); wherein: The driving component includes a drive needle control motor (121) detachably connected to the rotating needle control cover (12) and a pusher; the drive needle control motor (121) is used to drive the rotating needle control cover (12) to perform circumferential reciprocating motion; the lower side of the rotating needle control cover (12) is provided with a plurality of spiral transition grooves (122) respectively for matching with the corresponding radial needle control grooves (111); the pusher includes a spiral insert (134) inserted into the spiral transition groove (122), a push plate (133) inserted into the radial needle control groove (111), and a connecting post (135) connecting the spiral insert (134) and the push plate (133); a corresponding side of the push plate (133) is used to abut against a corresponding end of the microinjection needle to be punctured; the upper end of the spiral insert (134) is provided with an upper through hole (1341). The connecting post (135) is vertically connected to a lifting locking post (136) and has a side through groove (1351) on the outside. The upper end of the lifting locking post (136) protrudes from the upper through hole (1341), and the lower end abuts against a return spring (137) inserted in the connecting post (135). The lower front end is provided with an arc-shaped abutment elastic part (1361) protruding from the side through groove (1351). A rubber sleeve (13) is inserted into the radial needle control groove (111). The rubber sleeve (13) has an injection needle slot (131) and an upper opening (1311) on the upper side for the push plate (133) to move into the injection needle slot (131). The upper side of the rubber sleeve (13) is provided with a contact upper plane (132) that contacts the lower side of the rotating needle control cover (12). The fixed needle breaking mechanism (1) is provided with a vertical needle feeding mechanism (2) for driving the fixed needle breaking mechanism (1) to move to match the automatic needle breaking mechanism (5) on its lower side; the vertical needle feeding mechanism (2) includes a support plate (24) that is fixed to the fixed control needle lower plate (11); a motor frame (241) is provided on the lower side of the support plate (24), the drive control needle motor (121) is fixed in the motor frame (241), and the output end is sleeved with a fixed needle breaking bearing (14) located in the fixed control needle lower plate (11); the axes of the drive control needle motor (121), the fixed needle breaking bearing (14), the fixed control needle lower plate (11) and the rotating control needle cover (12) are coincident, and the motor frame (241) is provided with heat dissipation holes (242).

2. The needle-breaking device for a microinjection needle for Caenorhabditis elegans according to claim 1, characterized in that: The vertical needle feeding mechanism (2) further includes a rotating plug part (23), an external gear (21) and a threaded rod (22) connected sequentially from top to bottom; the rotating plug part (23) is sleeved with a vertical needle feeding bearing (25) located at the bottom of the motor frame (241); the external gear (21) is meshed with a spur tooth (43), and the threaded rod (22) is threadedly connected to a threaded connecting block (31).

3. The needle-breaking device for a microinjection needle for Caenorhabditis elegans according to claim 2, characterized in that: The threaded connecting block (31) is connected to the needle feeding telescopic device (3) and the needle breaking base (4); the straight tooth (43) is arranged in the horizontal direction and is connected and fixed to the fixing rod (431) which is connected and fixed to the needle breaking base (4).

4. The needle-breaking device for a microinjection needle for Caenorhabditis elegans according to claim 3, characterized in that: The needle-breaking base (4) is provided with a convex groove (41) along its length direction, and the bottom of the threaded connecting block (31) matches the convex groove (41); the threaded connecting block (31) is fixedly connected to a matching vertical plate (32), and the support plate (24) is provided with an abutting plane (243) for abutting against the matching vertical plate (32).

5. The needle-breaking device for a microinjection needle for Caenorhabditis elegans according to claim 4, characterized in that: The needle-breaking base (4) is provided with a support column (42) for connecting and fixing the automatic needle-breaking mechanism (5); the automatic needle-breaking mechanism (5) includes a dust cover (51) located on the lower side and a vacuum cleaner (52) and a rotating motor (53) located on the upper side. The dust cover (51) is provided with a matching window (511) at one end facing the fixed needle-breaking mechanism (1). The vacuum cleaner (52) is connected to multiple suction pipes (521) with equal arc distribution. The output end of the rotating motor (53) is connected to a rotating rod (531). The rotating rod (531) is connected to a grinding cutter (532). The rotating motor (53) is used to drive the grinding cutter (532) to rotate circumferentially along one side of the outer circumference of the fixed control needle lower plate (11).

6. The needle-breaking device for a microinjection needle for Caenorhabditis elegans according to claim 5, characterized in that: The matching vertical plate (32) is provided with a window fitting part (321) that matches the matching window (511) and a matching slot (322) through which the straight tooth (43) passes.

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